#METABOLOMICS WORKBENCH araskind_20160414_093817 DATATRACK_ID:591 STUDY_ID:ST000378 ANALYSIS_ID:AN000612 VERSION 1 CREATED_ON 03-03-2022 #PROJECT PR:PROJECT_TITLE IDH1(R132H) mutations activity in neurospheres PR:PROJECT_SUMMARY assay 2HG procution in neurospheres expressing IDH1 mutated PR:INSTITUTE University of Michigan PR:DEPARTMENT Neurosurgery PR:LABORATORY Castro Lab PR:LAST_NAME Castro PR:FIRST_NAME Maria PR:ADDRESS Ann Arbor, MI PR:EMAIL mariacas@umich.edu PR:PHONE 734-764-0850 PR:DOI http://dx.doi.org/10.21228/M8NP5K #STUDY ST:STUDY_TITLE 2 hydroxiglutarate with with IDH1-R132H inhibitor in neurospheres ST:STUDY_TYPE Regular ST:STUDY_SUMMARY Tumor neurospheres carrying genetic lession NPI (NRAS, shP53, IDH1-R132H) colony ST:STUDY_SUMMARY 1 and 2 (C1 and C2); NPAI (NRAS, shP53, shATRX, IDH1-R132H) colony 1 and 2 (C1 ST:STUDY_SUMMARY and C2); and NPA (NRAS, shP53, shATRX) (C1 and C2). Was treated with IDH1-R132H ST:STUDY_SUMMARY inhibitor. By 2HG assay we want to check the effect of the inhibitor in our ST:STUDY_SUMMARY cells. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:SUBMIT_DATE 2016-04-20 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012404 S00018624 shATRX plasmid:no | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012368 S00018487 shATRX plasmid:no | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018488 shATRX plasmid:no | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018489 shATRX plasmid:no | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018490 shATRX plasmid:no | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012404 S00018625 shATRX plasmid:no | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018495 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012368 S00018496 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012368 S00018497 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012368 S00018498 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012404 S00018620 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012404 S00018622 shATRX plasmid:yes | IDH1-R132H mutation:no SUBJECT_SAMPLE_FACTORS SU0012368 S00018491 shATRX plasmid:yes | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018492 shATRX plasmid:yes | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018493 shATRX plasmid:yes | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012368 S00018494 shATRX plasmid:yes | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012404 S00018621 shATRX plasmid:yes | IDH1-R132H mutation:yes SUBJECT_SAMPLE_FACTORS SU0012404 S00018623 shATRX plasmid:yes | IDH1-R132H mutation:yes #COLLECTION CO:COLLECTION_SUMMARY none CO:SAMPLE_TYPE Cell Line, Tumor #TREATMENT TR:TREATMENT_SUMMARY none #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME 15DEC2015_2HG_cells_Castro.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY none CH:METHODS_ID DQM0033 CH:METHODS_FILENAME 2015-04-07-2HG.m.zip CH:INSTRUMENT_NAME None CH:COLUMN_NAME Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE EX00452-2HG-QUANT-method.m.zip #MS MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS umol/g MS_METABOLITE_DATA_START Samples S00018487 S00018488 S00018489 S00018490 S00018495 S00018496 S00018497 S00018498 S00018491 S00018492 S00018493 S00018494 Factors shATRX plasmid:no | IDH1-R132H mutation:yes shATRX plasmid:no | IDH1-R132H mutation:yes shATRX plasmid:no | IDH1-R132H mutation:yes shATRX plasmid:no | IDH1-R132H mutation:yes shATRX plasmid:yes | IDH1-R132H mutation:no shATRX plasmid:yes | IDH1-R132H mutation:no shATRX plasmid:yes | IDH1-R132H mutation:no shATRX plasmid:yes | IDH1-R132H mutation:no shATRX plasmid:yes | IDH1-R132H mutation:yes shATRX plasmid:yes | IDH1-R132H mutation:yes shATRX plasmid:yes | IDH1-R132H mutation:yes shATRX plasmid:yes | IDH1-R132H mutation:yes D-2-hydroxyglutarate 29.9000 17.6600 47.8500 9.9000 1.7300 1.1900 3.5000 2.7100 41.7300 11.7100 103.6700 20.9200 L-2-hydroxyglutarate 2.3700 4.0600 2.5200 2.0900 1.3100 0.8200 1.4400 1.3500 1.9000 1.4400 1.8200 1.7100 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant D-2-hydroxyglutarate 439391 L-2-hydroxyglutarate 439939 METABOLITES_END #END