#METABOLOMICS WORKBENCH hormel101_20160715_082310_mwtab.txt DATATRACK_ID:679 STUDY_ID:ST000421 ANALYSIS_ID:AN000665 PROJECT_ID:PR000330 VERSION 1 CREATED_ON July 18, 2016, 9:14 am #PROJECT PR:PROJECT_TITLE Impact of Long-Term Poor and Good Glycemic Control on Metabolomics Alterations PR:PROJECT_TITLE in Type 1 Diabetic People. PR:PROJECT_TYPE Untargeted LC-MS Metabolomics PR:PROJECT_SUMMARY The objective of the study was to determine whether T1D with good glycemic PR:PROJECT_SUMMARY control have persistent abnormalities of metabolites and pathways that exist in PR:PROJECT_SUMMARY T1D with poor glycemic control. PR:INSTITUTE Mayo Clinic PR:DEPARTMENT Endocrinology PR:LABORATORY Mayo Clinic Metabolomics Resource Core PR:LAST_NAME Nair PR:FIRST_NAME Sreekumaran PR:ADDRESS 200 First Street SW, Rochester, MN 55905 PR:EMAIL Nair.K@mayo.edu PR:PHONE 507-285-2415 #STUDY ST:STUDY_TITLE ms3076 T1D poor glycemic control and control samples ST:STUDY_TYPE Plasma metabolites in T1 diabetes ST:STUDY_SUMMARY The objective of the study was to determine whether T1D with good glycemic ST:STUDY_SUMMARY control have persistent abnormalities of metabolites and pathways that exist in ST:STUDY_SUMMARY T1D with poor glycemic control. ST:INSTITUTE Mayo Clinic ST:DEPARTMENT Endocrinology ST:LABORATORY Mayo Clinic Metabolomics Resource Core ST:LAST_NAME Nair ST:FIRST_NAME Sreekumaran ST:ADDRESS 200 First Street SW, Rochester, MN 55905 ST:EMAIL Nair.K@mayo.edu ST:PHONE 507-285-2415 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS T1D_S16 21mar12_22-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S16 21mar12_22-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S16 13jan12_50-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S16 13jan12_50-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S17 21mar12_23-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S17 21mar12_23-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S17 21mar12_24-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S17 21mar12_24-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S18 21mar12_25-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S18 21mar12_25-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S18 21mar12_26-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S18 21mar12_26-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S19 21mar12_27-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S19 21mar12_27-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S19 13jan12_51-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S19 13jan12_51-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S20 21mar12_28-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S20 21mar12_28-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S20 21mar12_38-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S20 21mar12_38-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S21 21mar12_39-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S21 21mar12_39-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S21 21mar12_40-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S21 21mar12_40-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S22 21mar12_41-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S22 21mar12_41-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S22 21mar12_42-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S22 21mar12_42-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S23 21mar12_43-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S23 21mar12_43-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S23 21mar12_44-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S23 21mar12_44-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S24 21mar12_45-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S24 21mar12_45-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S24 13jan12_53-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S24 13jan12_53-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S25 21mar12_46-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S25 21mar12_46-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S25 13jan12_54-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S25 13jan12_54-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S26 21mar12_56-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S26 21mar12_56-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S26 21mar12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S26 21mar12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S27 21mar12_13-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S27 21mar12_13-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S27 13jan12_55-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S27 13jan12_55-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S28 21mar12_14-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S28 21mar12_14-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S28 13jan12_56-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S28 13jan12_56-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S29 21mar12_15-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S29 21mar12_15-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S29 13jan12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S29 13jan12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S16 23mar12_22-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S16 23mar12_22-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S16 09feb12_50-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S16 09feb12_50-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S17 23mar12_23-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S17 23mar12_23-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S17 23mar12_24-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S17 23mar12_24-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S18 23mar12_25-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S18 23mar12_25-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S18 23mar12_26-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S18 23mar12_26-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S19 23mar12_27-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S19 23mar12_27-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S19 09feb12_51-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S19 09feb12_51-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S20 23mar12_28-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S20 23mar12_28-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S20 23mar12_38-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S20 23mar12_38-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S21 23mar12_39-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S21 23mar12_39-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S21 23mar12_40-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S21 23mar12_40-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S22 23mar12_41-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S22 23mar12_41-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S22 23mar12_42-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S22 23mar12_42-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S23 23mar12_43-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S23 23mar12_43-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S23 23mar12_44-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S23 23mar12_44-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S24 23mar12_45-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S24 23mar12_45-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S24 09feb12_53-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S24 09feb12_53-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S25 23mar12_46-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S25 23mar12_46-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S25 09feb12_54-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S25 09feb12_54-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S26 23mar12_56-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S26 23mar12_56-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S26 23mar12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S26 23mar12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S27 23mar12_13-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S27 23mar12_13-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S27 09feb12_55-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S27 09feb12_55-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S28 23mar12_14-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S28 23mar12_14-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S28 09feb12_56-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S28 09feb12_56-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S29 23mar12_15-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S29 23mar12_15-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S29 09feb12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS ND_S29 09feb12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=hilic SUBJECT_SAMPLE_FACTORS T1D_S16 26mar12_22-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S16 26mar12_22-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S16 16feb12_50-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S17 26mar12_23-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S17 26mar12_23-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S17 26mar12_24-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S17 26mar12_24-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S18 26mar12_25-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S18 26mar12_25-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S18 26mar12_26-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S18 26mar12_26-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S19 26mar12_27-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S19 26mar12_27-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S19 16feb12_51-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S19 16feb12_51-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S20 26mar12_28-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S20 26mar12_28-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S20 26mar12_38-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S20 26mar12_38-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S21 26mar12_39-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S21 26mar12_39-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S21 26mar12_40-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S21 26mar12_40-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S22 26mar12_41-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S22 26mar12_41-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S22 26mar12_42-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S22 26mar12_42-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S23 26mar12_43-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S23 26mar12_43-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S23 26mar12_44-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S23 26mar12_44-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S24 26mar12_45-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S24 26mar12_45-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S24 16feb12_53-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S24 16feb12_53-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S25 26mar12_46-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S25 26mar12_46-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S25 16feb12_54-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S25 16feb12_54-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S26 26mar12_56-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S26 26mar12_56-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S26 26mar12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S26 26mar12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S27 26mar12_13-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S27 26mar12_13-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S27 16feb12_55-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S27 16feb12_55-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S28 26mar12_14-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S28 26mar12_14-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S28 16feb12_56-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S28 16feb12_56-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S29 26mar12_15-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S29 26mar12_15-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S29 16feb12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S29 16feb12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=positive; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S16 27mar12_22-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S16 27mar12_22-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S16 14feb12_50-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S16 14feb12_50-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S17 27mar12_23-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S17 27mar12_23-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S17 27mar12_24-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S17 27mar12_24-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S18 27mar12_25-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S18 27mar12_25-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S18 27mar12_26-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S18 27mar12_26-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S19 27mar12_27-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S19 27mar12_27-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S19 14feb12_51-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S19 14feb12_51-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S20 27mar12_28-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S20 27mar12_28-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S20 27mar12_38-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S20 27mar12_38-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S21 27mar12_39-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S21 27mar12_39-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S21 27mar12_40-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S21 27mar12_40-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S22 27mar12_41-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S22 27mar12_41-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S22 27mar12_42-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S22 27mar12_42-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S23 27mar12_43-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S23 27mar12_43-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S23 27mar12_44-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S24 27mar12_45-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S24 27mar12_45-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S24 14feb12_53-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S24 14feb12_53-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S25 27mar12_46-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S25 27mar12_46-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S25 14feb12_54-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S25 14feb12_54-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S26 27mar12_56-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S26 27mar12_56-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S26 27mar12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S26 27mar12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S27 27mar12_13-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S27 27mar12_13-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S27 14feb12_55-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S27 14feb12_55-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S28 27mar12_14-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S28 27mar12_14-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S28 14feb12_56-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S28 14feb12_56-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S29 27mar12_15-r001.d treatment:T1D poor glycemic control study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS T1D_S29 27mar12_15-r002.d treatment:T1D poor glycemic control study=ms3076; rep=2; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S29 14feb12_57-r001.d treatment:ND study=ms3076; rep=1; Ionization=negative; Separation=C18 SUBJECT_SAMPLE_FACTORS ND_S29 14feb12_57-r002.d treatment:ND study=ms3076; rep=2; Ionization=negative; Separation=C18 #COLLECTION CO:COLLECTION_SUMMARY At 5:00 AM after an overnight fast, baseline blood samples were collected from CO:COLLECTION_SUMMARY study participants. Plasma samples were stored at 80°C until analysis. CO:SAMPLE_TYPE Blood. Plasma was isolated for MS analysis. #TREATMENT TR:TREATMENT_SUMMARY Participants were admitted to the Clinical Research Unit at St Mary’s Hospital TR:TREATMENT_SUMMARY (Rochester, Minnesota) the evening before the study and spent overnight in the TR:TREATMENT_SUMMARY Clinical Research Unit. The participants were given a standard meal on the TR:TREATMENT_SUMMARY evening of the admission after which they fasted overnight. Participants with TR:TREATMENT_SUMMARY T1D were treated with insulin as per their usual individual programs. At 5:00 AM TR:TREATMENT_SUMMARY after an overnight fast, baseline blood samples were collected from study TR:TREATMENT_SUMMARY participants. Plasma samples were stored at 80°C until analysis. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Plasma quality-control samples used in the study were prepared from pooled SP:SAMPLEPREP_SUMMARY plasma spiked with a selection of metabolites to mimic elevated levels of SP:SAMPLEPREP_SUMMARY metabolites during I− (insulin withdrawn) condition. Plasma was spiked with a SP:SAMPLEPREP_SUMMARY standard mixture (3:1 ratio of plasma to spiking solution) containing 100 μg/mL SP:SAMPLEPREP_SUMMARY niacin, hypoxanthine, leucine, isoleucine, phenylalanine, tryptophan, citric SP:SAMPLEPREP_SUMMARY acid, glucose, hippuric acid, and taurocholic acid dissolved in 1:1 SP:SAMPLEPREP_SUMMARY acetonitrile/water. All plasma samples (200 μL) were thawed on ice at 4°C SP:SAMPLEPREP_SUMMARY followed by deproteinization with methanol (1:4 ratio of plasma to methanol) and SP:SAMPLEPREP_SUMMARY vortexed for 10 s, followed by incubation at −20°C for 2 h. The samples were SP:SAMPLEPREP_SUMMARY then centrifuged at 15,871g for 30 min at 4°C. The supernatants were SP:SAMPLEPREP_SUMMARY lyophilized (Savant, Holbrook, NY) and stored at −20°C prior to analysis. The SP:SAMPLEPREP_SUMMARY samples were reconstituted in 50% H2O/acetonitrile and passed through a Microcon SP:SAMPLEPREP_SUMMARY YM3 filter (Millipore Corporation). The supernatants were transferred to SP:SAMPLEPREP_SUMMARY analytical vials, stored in the autosampler at 4°C, and analyzed within 48 h of SP:SAMPLEPREP_SUMMARY reconstitution in buffer. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY The liquid chromatography platform consisted of an Acquity UPLC system (Waters, CH:CHROMATOGRAPHY_SUMMARY Milford, MA). Plasma metabolite separation was achieved using both hydrophilic CH:CHROMATOGRAPHY_SUMMARY interaction chromatography (ethylene-bridged hybrid 2.1 × 150 mm, 1.7 μm; CH:CHROMATOGRAPHY_SUMMARY Waters) and reversed-phase liquid chromatography C18 (high-strength silica 2.1 CH:CHROMATOGRAPHY_SUMMARY × 150 mm, 1.8 μm; Waters). For each column, the run time was 20 min at a flow CH:CHROMATOGRAPHY_SUMMARY rate of 400 μL/min. Reverse-phase chromatography was performed using 99% CH:CHROMATOGRAPHY_SUMMARY solvent A (5 mmol/L NH4 acetate, 0.1% formic acid, and 1% acetonitrile) to 100% CH:CHROMATOGRAPHY_SUMMARY solvent B (95% acetonitrile with 0.1% formic acid). The gradient was 0 min, 0% CH:CHROMATOGRAPHY_SUMMARY B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; CH:CHROMATOGRAPHY_SUMMARY and 20 min, 0% B. Other LC parameters were injection volume 5 μL and column CH:CHROMATOGRAPHY_SUMMARY temperature 50°C. Each sample was injected in triplicate with blank injections CH:CHROMATOGRAPHY_SUMMARY between each sample. Quality controls and standards were run at the beginning CH:CHROMATOGRAPHY_SUMMARY and end of the sequence. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 6220 CH:COLUMN_NAME None #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6220 TOF MS:INSTRUMENT_TYPE TOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:CAPILLARY_TEMPERATURE 300°C MS:CAPILLARY_VOLTAGE 3.5 kV MS:FRAGMENT_VOLTAGE 150 V MS:MASS_ACCURACY <5 parts per million and ~20000 respectively MS:NEBULIZER nebulizer gas 325°C MS:OCTPOLE_VOLTAGE 250 V MS:SCAN_RANGE_MOVERZ 50-1200 MS:SCANNING_CYCLE 0.5 s MS:SKIMMER_VOLTAGE 58 V MS:MS_RESULTS_FILE ST000421_AN000665_Results.txt UNITS:intensity Has m/z:Yes Has RT:Yes #END