#METABOLOMICS WORKBENCH hormel101_20161014_061947_mwtab.txt DATATRACK_ID:755 VERSION 1 CREATED_ON November 16, 2016, 3:16 pm #PROJECT PR:PROJECT_TITLE Mayo Metabolomics Pilot and Feasibility Award: Mechanisms of sleep apnea and PR:PROJECT_TITLE cardiovascular disease PR:PROJECT_TYPE MS targeted analysis PR:PROJECT_SUMMARY Targeted Metabolic and Biological Markers of Sleepiness Symptoms in Sleep apnea PR:PROJECT_SUMMARY and cardiovascular disease PR:INSTITUTE Mayo Clinic PR:LABORATORY Mayo Clinic Metabolomics Resource Core PR:LAST_NAME Pak PR:FIRST_NAME Victoria PR:ADDRESS unknown PR:EMAIL victoria.pak@yale.edu PR:PHONE 203-737-5065 #STUDY ST:STUDY_TITLE Sleep apnea and cardiovascular metabiltes carnitine, tma, tmao, betain, choline ST:STUDY_TITLE in plasma ST:STUDY_SUMMARY Sleep apnea and cardiovascular metabiltes carnitine, tma, tmao, betain, choline ST:STUDY_SUMMARY in plasma ST:INSTITUTE Mayo Clinic ST:LABORATORY Mayo Clinic Metabolomics Resource Core ST:LAST_NAME Pak ST:FIRST_NAME Victoria ST:ADDRESS unknown ST:EMAIL victoria.pak@yale.edu ST:PHONE 203-737-5065 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS s_01 ms5666-1 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_02 ms5666-2 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_03 ms5666-3 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_04 ms5666-4 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_05 ms5666-5 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_06 ms5666-6 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_07 ms5666-7 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_08 ms5666-8 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_09 ms5666-9 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_10 ms5666-10 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_11 ms5666-11 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_12 ms5666-12 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_13 ms5666-13 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_14 ms5666-14 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_15 ms5666-15 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_16 ms5666-16 Disease:cardiovascular disease sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_17 ms5666-17 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_18 ms5666-18 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_19 ms5666-19 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_20 ms5666-20 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_21 ms5666-21 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_22 ms5666-22 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_23 ms5666-23 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_24 ms5666-24 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_25 ms5666-25 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_26 ms5666-26 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_27 ms5666-27 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_28 ms5666-28 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_29 ms5666-29 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_30 ms5666-30 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_31 ms5666-31 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_32 ms5666-32 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_33 ms5666-33 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_34 ms5666-34 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_35 ms5666-35 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma SUBJECT_SAMPLE_FACTORS s_36 ms5666-36 Disease:sleep apnea sample type=plasma; Assay=carnitine, tma, tmao, betain, choline in plasma #COLLECTION CO:COLLECTION_SUMMARY For the metabolic markers, 9 ml blood will be drawn into 3 EDTA tubes (3 ml CO:COLLECTION_SUMMARY each) and gently centrifuged at 4°C for 10 min; plasma aliquots were CO:COLLECTION_SUMMARY immediately frozen and stored at -80 °C; will be stored at -80ºC in the CTRC CO:COLLECTION_SUMMARY until all samples are collected and then sent to Mayo Clinic Metabolomics Core CO:COLLECTION_SUMMARY via LC-MS/MS for simultaneous detection and quantification of metabolites for CO:COLLECTION_SUMMARY analyses. #TREATMENT TR:TREATMENT_SUMMARY Recruitment of subjects will take place from the clinic population in TR:TREATMENT_SUMMARY collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will TR:TREATMENT_SUMMARY be solicited for via an overnight sleep study, which is general monitoring of TR:TREATMENT_SUMMARY sleep and variety of body functions during sleep. This includes brain (EEG), eye TR:TREATMENT_SUMMARY (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be TR:TREATMENT_SUMMARY established by full sleep study. First, the PI will screen for eligibility and TR:TREATMENT_SUMMARY obtain informed consent. We will assess height, weight, medical history, TR:TREATMENT_SUMMARY demographics. EDS will be measured subjectively on the night of enrollment in TR:TREATMENT_SUMMARY the study and determined using the Epworth Sleepiness Scale. The information TR:TREATMENT_SUMMARY regarding gender, BMI, smoking, age, medications (name/duration/frequency), and TR:TREATMENT_SUMMARY presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of TR:TREATMENT_SUMMARY myocardial infarction, stroke, or heart failure) will then be obtained via the TR:TREATMENT_SUMMARY electronic medical record. We will also ask subjects whether they exercise by TR:TREATMENT_SUMMARY asking participants the yes/no question: “During the past month, did you TR:TREATMENT_SUMMARY participate in any physical activities or exercises?” Second, the subject will TR:TREATMENT_SUMMARY undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, TR:TREATMENT_SUMMARY biological samples will be collected in the morning after the sleep study. TR:TREATMENT_SUMMARY Patients who have agreed to participate will undergo a blood draw (20 ml) and TR:TREATMENT_SUMMARY urine collection (30 ml) on the morning after PSG. Laboratory analysis of the TR:TREATMENT_SUMMARY biological samples will be conducted in collaboration with the Clinical TR:TREATMENT_SUMMARY Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice TR:TREATMENT_SUMMARY sessions with the PVT prior to administration of the task. PVT test will be TR:TREATMENT_SUMMARY conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically TR:TREATMENT_SUMMARY scored. This will be done on the day after the sleep study. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY TMAO panel #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Cohesive TX2 CH:COLUMN_NAME Altma HP HILIC, 2.1x150mm, 5um #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME ABI Sciex 6500 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS micromolar MS_METABOLITE_DATA_START Samples ms5666-1 ms5666-2 ms5666-3 ms5666-4 ms5666-5 ms5666-6 ms5666-7 ms5666-8 ms5666-9 ms5666-10 ms5666-11 ms5666-12 ms5666-13 ms5666-14 ms5666-15 ms5666-16 ms5666-17 ms5666-18 ms5666-19 ms5666-20 ms5666-21 ms5666-22 ms5666-23 ms5666-24 ms5666-25 ms5666-26 ms5666-27 ms5666-28 ms5666-29 ms5666-30 ms5666-31 ms5666-32 ms5666-33 ms5666-34 ms5666-35 ms5666-36 Factors Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea carnitine 39.46 41.24 36.91 44.49 34.95 28.76 54.15 45.17 36.25 56.23 46.38 51.98 27.24 22.48 27.25 36.68 32.64 38.86 49.87 45.99 43.39 52.78 30.56 25.44 41.9 41.52 37.44 34.6 47.09 37 37.98 35.04 43.54 40.89 35.86 26.55 tma 13.77 14.44 12.54 15.51 12.24 9.811 18.88 16.06 12.69 20.71 16.1 18.69 9.965 8.641 10.14 14.04 12.31 14.92 18.51 17.17 16.14 19.64 11.48 9.5 15.64 15.23 13.29 12.7 17.86 13.87 13.39 12.51 16.65 15.19 13.31 9.584 tmao 1.441 7.597 2.137 3.061 1.646 1.997 1.686 0.9114 4.703 10.6 3.525 1.184 2.652 1.54 54.59 5.672 4.035 3.793 2.897 3.956 1.898 2.212 1.399 2.81 2.86 4.06 1.667 1.26 2.475 3.091 2.64 1.34 3.834 4.051 0.9078 1.1 betaine 48.53 36.03 23.07 34.82 44.9 33.32 47.06 38.51 33.75 39.12 40.7 46.67 25.01 27.76 45.03 34.65 31.17 41.45 47.01 45.12 42.68 42.87 28.55 23.64 35.04 38.16 37.91 35.16 52.96 32.67 36.66 42.54 48.66 49.08 38.38 32.99 choline 8.832 12.44 5.383 6.353 7.254 6.56 8.065 7.229 9.171 13.41 6.179 10.74 7.379 7.648 8.775 8.089 12.01 7.432 13.34 13.98 12.68 6.85 9.432 9.025 10.73 10.15 5.806 9.451 8.109 10.59 5.902 4.651 6.897 7.986 9.224 6.941 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name carnitine tma tmao betaine choline METABOLITES_END #END