#METABOLOMICS WORKBENCH amitch_20161011_121205 DATATRACK_ID:752 STUDY_ID:ST000498 ANALYSIS_ID:AN000766 PROJECT_ID:PR000375 VERSION 1 CREATED_ON October 31, 2016, 10:41 am #PROJECT PR:PROJECT_TITLE Drug's effect on cerebral metabolic pool sizes PR:PROJECT_SUMMARY The goal of this project is to analyze the metabolic pool distribution of PR:PROJECT_SUMMARY cerebral metabolites in response to drug administration and withdrawal PR:INSTITUTE University of Florida PR:DEPARTMENT Biochemistry and Molecular Biology PR:LAST_NAME Joanna PR:FIRST_NAME Long PR:ADDRESS 1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611 PR:EMAIL jrlong@mbi.ufl.edu PR:PHONE none PR:FUNDING_SOURCE UF #STUDY ST:STUDY_TITLE Cocaine administration and withdrawal ST:STUDY_TYPE treated and control ST:STUDY_SUMMARY The goal of this project is to analyze the metabolic pool distribution of ST:STUDY_SUMMARY cerebral metabolites in response to cocaine administration and withdrawal as ST:STUDY_SUMMARY compared to control ST:INSTITUTE University of Florida ST:DEPARTMENT SECIM ST:LAST_NAME Daniel ST:FIRST_NAME Downes ST:ADDRESS 1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611 ST:EMAIL daniel.downes@neurosurgery.ufl.edu ST:PHONE (321)303-6523 ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 12 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 SU:SUBJECT_COMMENTS Long Evans Rat #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS LongEvans1 LongDNP_001 drug admin:acute body weight=339g SUBJECT_SAMPLE_FACTORS LongEvans2 LongDNP_002 drug admin:acute body weight=402g SUBJECT_SAMPLE_FACTORS LongEvans3 LongDNP_003 drug admin:acute body weight=363g SUBJECT_SAMPLE_FACTORS LongEvans4 LongDNP_004 drug admin:acute control body weight=425g SUBJECT_SAMPLE_FACTORS LongEvans5 LongDNP_005 drug admin:acute control body weight=380g SUBJECT_SAMPLE_FACTORS LongEvans6 LongDNP_006 drug admin:acute control body weight=397g SUBJECT_SAMPLE_FACTORS LongEvans7 LongDNP_007 drug admin:withdrawal body weight=447g SUBJECT_SAMPLE_FACTORS LongEvans8 LongDNP_008 drug admin:withdrawal body weight=375g SUBJECT_SAMPLE_FACTORS LongEvans9 LongDNP_009 drug admin:withdrawal body weight=373g SUBJECT_SAMPLE_FACTORS LongEvans10 LongDNP_010 drug admin:withdrawal control body weight=385g SUBJECT_SAMPLE_FACTORS LongEvans11 LongDNP_011 drug admin:withdrawal control body weight=407g SUBJECT_SAMPLE_FACTORS LongEvans12 LongDNP_012 drug admin:withdrawal control body weight=434g #COLLECTION CO:COLLECTION_SUMMARY harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2 CO:SAMPLE_TYPE Tissue #TREATMENT TR:TREATMENT_SUMMARY Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, TR:TREATMENT_SUMMARY Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, TR:TREATMENT_SUMMARY Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME SB_OA_Assay.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase MS:INSTRUMENT_NAME Thermo Quantiva QQQ CH:COLUMN_NAME Waters Acquity BEH C18 (100 x 2mm, 1.7um) CH:METHODS_FILENAME SB_OA_Assay.pdf #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME Sanford Burnham Prebys #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantiva QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg dry powder MS_METABOLITE_DATA_START Samples LongDNP_004 LongDNP_005 LongDNP_006 LongDNP_001 LongDNP_002 LongDNP_003 LongDNP_010 LongDNP_011 LongDNP_012 LongDNP_007 LongDNP_008 LongDNP_009 Factors drug admin:acute control drug admin:acute control drug admin:acute control drug admin:acute drug admin:acute drug admin:acute drug admin:withdrawal control drug admin:withdrawal control drug admin:withdrawal control drug admin:withdrawal drug admin:withdrawal drug admin:withdrawal Lactate 58.99 61.21 57.85 67.32 73.85 62.09 54.93 65.42 60.16 46.36 50.16 65.27 Pyruvate 0.07 0.07 0.07 0.08 0.08 0.09 0.06 0.05 0.07 0.06 0.06 0.07 Succinate 7.17 1.80 2.37 2.17 2.12 1.96 2.04 2.64 1.83 2.13 1.90 2.21 Fumarate 0.14 0.15 0.16 0.18 0.16 0.16 0.16 0.10 0.13 0.19 0.14 0.17 Malate 0.80 1.03 1.13 1.18 1.02 1.10 1.11 0.66 0.89 1.26 1.05 1.17 a-KG 0.02 0.01 0.01 0.02 0.01 0.02 0.01 0.01 0.01 0.01 0.01 0.02 Citrate 0.37 0.53 0.49 0.55 0.60 0.51 0.51 0.30 0.48 0.59 0.40 0.51 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID Lactate 3.98 196.1, 91.062 107689 Pyruvate 6.68 299.1, 91.186 1060 Succinate 5.44 329.1, 206.233 1110 Fumarate 5.59 327.1, 91.192 444972 Malate 5.28 345.1, 91.18 525 a-KG 6.59 462.2, 91.2 164533 Citrate 5.95 508.25, 385 311 METABOLITES_END #END