#METABOLOMICS WORKBENCH hormel101_20161214_145452_mwtab.txt DATATRACK_ID:801 STUDY_ID:ST000526 ANALYSIS_ID:AN000804 PROJECT_ID:PR000387 VERSION 1 CREATED_ON January 5, 2017, 1:20 pm #PROJECT PR:PROJECT_TITLE Mayo Metabolomics Pilot and Feasibility Award: Effects of Curcumin PR:PROJECT_TITLE Supplementation on the Plasma Metabolome of Older Adults: Relation to Vascular PR:PROJECT_TITLE Function PR:PROJECT_SUMMARY Age is the major risk factor for cardiovascular diseases (CVD). Two key PR:PROJECT_SUMMARY contributors to the increased risk of CVD in middle-aged and older (MA/O) adults PR:PROJECT_SUMMARY are stiffening of the large elastic arteries and the development of vascular PR:PROJECT_SUMMARY endothelial dysfunction, indicated by impaired nitric oxide (NO)-induced PR:PROJECT_SUMMARY endothelium-dependent dilation (EDD). The mechanisms by which aging causes PR:PROJECT_SUMMARY arterial dysfunction are incompletely understood, but involve reductions in NO PR:PROJECT_SUMMARY bioavailability associated with the development of oxidative stress and PR:PROJECT_SUMMARY inflammation. Thus, establishing novel strategies to reduce arterial stiffness PR:PROJECT_SUMMARY and increase vascular endothelial function in MA/O adults by increasing NO PR:PROJECT_SUMMARY bioavailability and reducing oxidative stress and inflammation are a high PR:PROJECT_SUMMARY biomedical research priority. Curcumin is a naturally occurring phenol found in PR:PROJECT_SUMMARY the Indian spice turmeric that improves physiological function in animal models PR:PROJECT_SUMMARY of age-related diseases and is a promising nutraceutical for intervention for PR:PROJECT_SUMMARY promoting healthy aging. Our preclinical results indicate that chow supplemented PR:PROJECT_SUMMARY with curcumin reduces aortic pulse wave velocity (PWV), the most common and PR:PROJECT_SUMMARY clinically important measure of large elastic artery stiffness, restores PR:PROJECT_SUMMARY NO-mediated EDD and reduces arterial oxidative stress and inflammation in old PR:PROJECT_SUMMARY C57/BL6 mice. Preliminary data from our recently funded NIH R21 pilot grant PR:PROJECT_SUMMARY indicate that curcumin supplementation improves vascular function in humans. It PR:PROJECT_SUMMARY is possible that changes in the circulating (plasma) metabolome with oral PR:PROJECT_SUMMARY curcumin supplementation will provide insight into novel metabolic mechanisms by PR:PROJECT_SUMMARY which curcumin may improve vascular function. The goal of this project is to PR:PROJECT_SUMMARY identify key metabolic pathways that change with oral curcumin supplementation PR:PROJECT_SUMMARY and to relate those changes with improvements in vascular function in MA/O PR:PROJECT_SUMMARY adults with initial endothelial dysfunction. Metabolomic analysis of plasma PR:PROJECT_SUMMARY samples at baseline also may produce unique molecular signatures that predict PR:PROJECT_SUMMARY responsiveness (changes in vascular function) to curcumin supplementation among PR:PROJECT_SUMMARY individuals. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Seals PR:FIRST_NAME Douglas PR:ADDRESS Department of Integrative Physiology University of Colorado Boulder, CO 80309 PR:EMAIL seals@colorado.edu PR:PHONE 303-492-5305 #STUDY ST:STUDY_TITLE Effects of Curcumin Supplementation on the Ceramides Concentration of Older ST:STUDY_TITLE Adults: Relation to Vascular Function ST:STUDY_SUMMARY Perform ceramides concentrations metabolite analysis related to nitric oxide ST:STUDY_SUMMARY biology, oxidative stress and inflammation in plasma before and after 12 weeks ST:STUDY_SUMMARY of oral curcumin (2000 mg/d) or placebo (double-blind, randomized) in men and ST:STUDY_SUMMARY women aged 45-79 years who are free from clinical cardiovascular disease. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Seals ST:FIRST_NAME Douglas ST:ADDRESS Department of Integrative Physiology University of Colorado Boulder, CO 80309 ST:EMAIL seals@colorado.edu ST:PHONE 303-492-5305 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS IF131 ms5533-1 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU02 ms5533-2 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU02 ms5533-3 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU06 ms5533-4 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU06 ms5533-5 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS IF145 ms5533-6 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU10 ms5533-7 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU10 ms5533-8 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU11 ms5533-9 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU11 ms5533-10 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS IF119 ms5533-11 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU14 ms5533-12 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU14 ms5533-13 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU20 ms5533-14 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU20 ms5533-15 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9758 ms5533-16 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU22 ms5533-17 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU22 ms5533-18 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU23 ms5533-19 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU23 ms5533-20 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU24 ms5533-21 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU24 ms5533-22 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9737 ms5533-23 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU28 ms5533-24 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU28 ms5533-25 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU31 ms5533-26 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU31 ms5533-27 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU33 ms5533-28 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU33 ms5533-29 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 6218 ms5533-30 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU34 ms5533-31 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU34 ms5533-32 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU36 ms5533-33 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU36 ms5533-34 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU40 ms5533-35 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU40 ms5533-36 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS EC96 ms5533-37 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS 9732 ms5588-1 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU46 ms5588-2 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU46 ms5588-3 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU48 ms5588-4 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU48 ms5588-5 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9735 ms5588-6 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU49 ms5588-7 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU49 ms5588-8 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU51 ms5588-9 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU51 ms5588-10 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS 9760 ms5588-11 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU52 ms5588-12 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU52 ms5588-13 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU54 ms5588-14 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU54 ms5588-15 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9727 ms5588-16 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU56 ms5588-17 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU56 ms5588-18 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU57 ms5588-19 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU57 ms5588-20 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU58 ms5588-21 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU58 ms5588-22 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS IF152 ms5588-23 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU60 ms5588-24 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU60 ms5588-25 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU63 ms5588-26 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU63 ms5588-27 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU65 ms5588-28 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU65 ms5588-29 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9759 ms5588-30 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU66 ms5588-31 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU66 ms5588-32 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU71 ms5588-33 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU71 ms5588-34 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU75 ms5588-35 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU75 ms5588-36 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS IF182 ms5588-37 group:young | Time point:Baseline #COLLECTION CO:COLLECTION_SUMMARY Healthy men & women aged 45-79 without clinical CVD, but with below normal CO:COLLECTION_SUMMARY baseline endothelial dysfunction (FMDBA < 7%). #TREATMENT TR:TREATMENT_SUMMARY "A 12-week randomized, double-blind, placebo controlled study will be conducted. TR:TREATMENT_SUMMARY After CTRC screening for inclusion/exclusion criteria, qualified subjects will TR:TREATMENT_SUMMARY be randomly assigned to 1 of 2 groups. The investigators involved in the TR:TREATMENT_SUMMARY acquisition and analysis of key outcomes will be blinded to the curcumin intake TR:TREATMENT_SUMMARY status of the subjects. With the assistance of dietary monitoring from the TR:TREATMENT_SUMMARY UC-Boulder CTRC bionutritionists, subjects will maintain their baseline diet TR:TREATMENT_SUMMARY with either unchanged (control) or enhanced curcumin intake delivered as TR:TREATMENT_SUMMARY capsules (Longvida®, Verdure Sciences): Group 1 = placebo (inert substances); TR:TREATMENT_SUMMARY Group 2 = curcumin (2000mg curcumin/day). Extensive published work has TR:TREATMENT_SUMMARY established that the curcumin dose of 2000 mg/day is well tolerated and safe. TR:TREATMENT_SUMMARY Sessions 1 & 2: Screening measurements. Session 3: Baseline measurements and TR:TREATMENT_SUMMARY blood draw. Sessions 4-8 (every other week to assess adherence and overall TR:TREATMENT_SUMMARY subject well-being): Body weight, BP, adherence, discuss any problems. Session TR:TREATMENT_SUMMARY 9: Identical to session 3 (stop intake of capsules after completion of TR:TREATMENT_SUMMARY post-testing)." #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Venous blood sampling. All blood samples are handled in a similar time frame and SP:SAMPLEPREP_SUMMARY immediately spun to extract plasma and stored in our -80 freezer until ready for SP:SAMPLEPREP_SUMMARY analysis. Ceramides concentrations in plasma. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME AMT HALO HILIC 2.7, 4.6x150mm #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantiva QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples ms5533-1 ms5533-2 ms5533-3 ms5533-4 ms5533-5 ms5533-6 ms5533-7 ms5533-8 ms5533-9 ms5533-10 ms5533-11 ms5533-12 ms5533-13 ms5533-14 ms5533-15 ms5533-16 ms5533-17 ms5533-18 ms5533-19 ms5533-20 ms5533-21 ms5533-22 ms5533-23 ms5533-24 ms5533-25 ms5533-26 ms5533-27 ms5533-28 ms5533-29 ms5533-30 ms5533-31 ms5533-32 ms5533-33 ms5533-34 ms5533-35 ms5533-36 ms5533-37 ms5588-1 ms5588-2 ms5588-3 ms5588-4 ms5588-5 ms5588-6 ms5588-7 ms5588-8 ms5588-9 ms5588-10 ms5588-11 ms5588-12 ms5588-13 ms5588-14 ms5588-15 ms5588-16 ms5588-17 ms5588-18 ms5588-19 ms5588-20 ms5588-21 ms5588-22 ms5588-23 ms5588-24 ms5588-25 ms5588-26 ms5588-27 ms5588-28 ms5588-29 ms5588-30 ms5588-31 ms5588-32 ms5588-33 ms5588-34 ms5588-35 ms5588-36 ms5588-37 Factors group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline S1P 1.085302796 0.706659741 1.534611825 0.725644629 0.859593565 0.640212631 0.488333522 0.620173026 0.745684234 0.835335096 0.671854112 0.959791588 1.701256958 1.055770747 0.724589913 0.663416383 0.639157915 0.538959892 0.800529467 0.628610754 0.517865571 1.109561264 0.66974468 0.401846808 0.438761869 0.522084435 0.570601373 0.55794478 0.532631596 0.524193867 0.709823889 0.492552386 0.46512977 0.474622214 0.554780632 1.036785858 0.573765521 0.26578844 0.776270999 0.54739762 0.588531545 0.588531545 0.594859841 0.60857115 0.498880683 0.477786362 0.473567498 0.528412731 1.125382005 0.513646707 0.529467448 0.42505056 0.811076628 0.421886412 0.530522164 1.228744176 0.607516434 0.690839 0.526303299 0.541069324 0.565327793 0.417667548 0.434543005 0.373369475 0.524193867 0.453527893 0.930259539 0.528412731 0.519975003 0.495716535 0.406065672 0.623337174 0.614899446 0.573765521 SPA 0.009293125 0.007965536 0.027879375 0.005310357 0.007965536 0.007965536 0.005310357 0.007965536 0.007965536 0.011948304 0.009293125 0.010620714 0.022569018 0.010620714 0.009293125 0.006637946 0.006637946 0.005310357 0.007965536 0.007965536 0.005310357 0.011948304 0.011948304 0.003982768 0.003982768 0.003982768 0.003982768 0.005310357 0.003982768 0.005310357 0.007965536 0.006637946 0.003982768 0.005310357 0.005310357 0.013275893 0.006637946 0.002655179 0.006637946 0.003982768 0.003982768 0.002655179 0.007965536 0.003982768 0.002655179 0.003982768 0.003982768 0.002655179 0.010620714 0.003982768 0.003982768 0.003982768 0.022569018 0.001327589 0.002655179 0.014603482 0.003982768 0.005310357 0.003982768 0.002655179 0.002655179 0.003982768 0.002655179 0.002655179 0.003982768 0.003982768 0.010620714 0.003982768 0.003982768 0.003982768 0.002655179 0.006637946 0.003982768 0.005310357 Sph 0.033413303 0.018711449 0.093557247 0.017374917 0.033413303 0.017374917 0.010692257 0.018711449 0.022721046 0.030740238 0.02539411 0.033413303 0.112268696 0.040095963 0.02539411 0.016038385 0.016038385 0.012028789 0.017374917 0.018711449 0.009355725 0.058807412 0.026730642 0.008019193 0.009355725 0.009355725 0.010692257 0.013365321 0.010692257 0.009355725 0.020047982 0.013365321 0.009355725 0.012028789 0.014701853 0.049451688 0.008019193 0.005346128 0.014701853 0.009355725 0.009355725 0.008019193 0.016038385 0.009355725 0.008019193 0.010692257 0.008019193 0.006682661 0.029403706 0.008019193 0.012028789 0.008019193 0.038759431 0.005346128 0.005346128 0.036086367 0.008019193 0.009355725 0.005346128 0.008019193 0.005346128 0.012028789 0.005346128 0.005346128 0.008019193 0.006682661 0.022721046 0.008019193 0.010692257 0.008019193 0.006682661 0.009355725 0.008019193 0.010692257 C8-cer C14-Cer 0.010991578 0.006280902 0.00863624 0.007066014 0.00863624 0.013346916 0.009421352 0.010206465 0.013346916 0.013346916 0.009421352 0.010991578 0.015702254 0.012561803 0.013346916 0.014917141 0.009421352 0.005495789 0.007066014 0.007851127 0.015702254 0.021198043 0.010991578 0.012561803 0.01177669 0.009421352 0.010991578 0.014132028 0.013346916 0.010206465 0.012561803 0.010991578 0.013346916 0.010991578 0.014132028 0.013346916 0.01177669 0.003140451 0.010991578 0.010206465 0.007851127 0.007851127 0.010991578 0.007066014 0.007066014 0.009421352 0.009421352 0.003140451 0.010206465 0.01177669 0.01177669 0.010206465 0.003925563 0.007851127 0.00863624 0.006280902 0.007066014 0.006280902 0.005495789 0.006280902 0.007851127 0.00863624 0.007066014 0.00863624 0.007066014 0.005495789 0.010991578 0.007066014 0.006280902 0.010991578 0.007851127 0.009421352 0.007066014 0.010991578 C16-Cer 0.174135647 0.216553305 0.250040929 0.27534269 0.363154683 0.311806992 0.273854351 0.261203471 0.250040929 0.246320082 0.206879102 0.299156112 0.349759633 0.284272723 0.305109467 0.256738454 0.306597806 0.24855259 0.218041644 0.197949069 0.376549733 0.397386477 0.28873774 0.230692524 0.221018321 0.322969534 0.305853637 0.334132075 0.335620414 0.234413371 0.318504517 0.311062823 0.320737025 0.307341976 0.365387191 0.340085431 0.276831029 0.075161113 0.322225364 0.292458587 0.231436694 0.232180863 0.224739169 0.337852922 0.273110182 0.285016893 0.303621128 0.156275581 0.259715132 0.278319368 0.283528554 0.255250115 0.142136362 0.212088288 0.235157541 0.162228936 0.213576627 0.216553305 0.24855259 0.15776392 0.273110182 0.317016178 0.19646073 0.20985578 0.250040929 0.218785813 0.203158255 0.269389335 0.257482624 0.23664588 0.215064966 0.292458587 0.272366012 0.256738454 C18:1-Cer 0.004968697 0.009227581 0.010647208 0.008517767 0.009227581 0.008517767 0.011357022 0.011357022 0.009227581 0.008517767 0.007098139 0.006388325 0.006388325 0.007098139 0.007098139 0.006388325 0.007807953 0.006388325 0.006388325 0.005678511 0.008517767 0.008517767 0.009227581 0.007098139 0.007807953 0.009227581 0.007098139 0.009227581 0.009227581 0.009227581 0.008517767 0.009227581 0.006388325 0.004968697 0.009227581 0.008517767 0.008517767 0.000709814 0.006388325 0.005678511 0.006388325 0.005678511 0.001419628 0.004258883 0.004258883 0.004258883 0.003549069 0.001419628 0.004258883 0.003549069 0.005678511 0.004258883 0.003549069 0.002129442 0.002129442 0.002839256 0.002129442 0.002129442 0.005678511 0.002839256 0.002839256 0.006388325 0.002129442 0.002129442 0.004968697 0.002839256 0.003549069 0.003549069 0.003549069 0.003549069 0.000709814 0.003549069 0.003549069 0.002839256 C18-Cer 0.036778813 0.136505977 0.140042402 0.057997358 0.117409286 0.092654316 0.115287432 0.090532462 0.171162935 0.152066244 0.042437091 0.065777492 0.093361601 0.076386765 0.079923189 0.070728486 0.07426491 0.057290073 0.103263589 0.079923189 0.146407965 0.164090087 0.086996037 0.078508619 0.086996037 0.091947031 0.105385444 0.131554983 0.121652995 0.053753649 0.183186778 0.185308633 0.081337759 0.07567948 0.146407965 0.120238426 0.096190741 0.014852982 0.106800013 0.092654316 0.142164256 0.142871541 0.074972195 0.094776171 0.088410607 0.066484777 0.076386765 0.045973516 0.089117892 0.080630474 0.093361601 0.084166898 0.080630474 0.084166898 0.114580147 0.039607952 0.053046364 0.050217225 0.067899346 0.044558946 0.04950994 0.079923189 0.045266231 0.038900667 0.046680801 0.045973516 0.089825177 0.077801334 0.090532462 0.079923189 0.070021201 0.135091408 0.140042402 0.078508619 C20-Cer 0.042454618 0.107821253 0.122646675 0.084235354 0.114560081 0.088278651 0.080192057 0.075474877 0.115907847 0.125342206 0.041106853 0.083561471 0.121298909 0.101756307 0.088278651 0.065366634 0.088278651 0.070757697 0.082213705 0.076822642 0.132081034 0.155666933 0.100408541 0.082213705 0.086257002 0.074127111 0.087604768 0.123320558 0.10243019 0.070757697 0.162405762 0.145558691 0.101082424 0.099734659 0.126016089 0.116581729 0.109842901 0.018868719 0.117255612 0.108495135 0.112538432 0.123320558 0.068736049 0.090974182 0.082887588 0.073453228 0.088952533 0.049193446 0.083561471 0.072779345 0.070757697 0.068736049 0.048519564 0.088952533 0.105125721 0.060649455 0.067388283 0.064692752 0.07143158 0.043128501 0.0667144 0.086930885 0.056606158 0.050541212 0.074800994 0.074800994 0.078844291 0.082887588 0.077496525 0.063344986 0.054584509 0.097039127 0.09771301 0.077496525 C22-cer 0.17567398 0.3005119 0.301798889 0.307590339 0.421488853 0.256754278 0.268337178 0.278633089 0.273485134 0.323677699 0.243240895 0.273485134 0.301155394 0.266406695 0.245814873 0.236805951 0.274128628 0.254823795 0.285711528 0.266406695 0.302442383 0.3120948 0.350704466 0.223936062 0.214283646 0.2657632 0.272841639 0.323034205 0.301155394 0.375800748 0.348130488 0.344913016 0.211066174 0.211709668 0.235518962 0.253536806 0.317886249 0.116472492 0.315312272 0.332686621 0.288929 0.288285506 0.23809294 0.272198145 0.235518962 0.234875468 0.240666918 0.202057252 0.254180301 0.254823795 0.22071859 0.245814873 0.189187363 0.238736434 0.249032345 0.250319334 0.289572494 0.275415617 0.310807811 0.180821935 0.286355022 0.304372866 0.225866546 0.200770263 0.323677699 0.346843499 0.224579557 0.261902234 0.272841639 0.197552791 0.194978813 0.264476212 0.272198145 0.240023423 C24:1-Cer 0.498438904 0.899290018 0.743643669 0.470644913 0.845554969 0.795525785 0.643585301 0.5793503 0.507085924 0.616408955 0.478674288 0.641732369 0.735614294 0.655938186 0.645438234 0.406409912 0.807261026 0.736231938 0.874584248 0.559585684 0.582438521 0.678791023 0.657173475 0.677555735 0.764025929 0.638026503 0.743026024 0.725731986 0.674467513 0.605291358 0.785643477 0.878290114 0.676320446 0.588614964 0.713996745 0.646055878 0.67693809 0.203204956 0.402704046 0.529938761 0.71029088 0.760937707 0.688673331 0.687438043 0.605291358 0.549703376 0.571938569 0.397762893 0.576879723 0.606526647 0.609614868 0.58367381 0.449645009 0.996260164 0.908554682 0.418762797 0.482380154 0.699173283 0.626908907 0.44840972 0.46138025 0.786261121 0.509556501 0.53426227 0.449027365 0.555262175 0.42802746 0.651614676 0.882613623 0.568232704 0.534879915 0.59973256 0.68990862 0.468174336 C24-Cer 0.999943045 1.549788574 1.695100495 1.478979799 1.926614402 1.578727813 1.613824336 1.737585759 1.559024501 1.483905627 1.449424833 1.713572349 1.937697514 1.669239899 1.675397183 1.51900215 1.663082614 1.496835925 2.060227481 2.073773507 1.915531289 1.985724335 1.64461076 1.344135263 1.160648177 1.739432945 1.655078144 1.696331952 1.548557117 2.273269534 1.978951322 1.953706455 2.020820858 1.935850329 1.414944038 1.45866076 1.672318541 0.779512251 2.148276653 2.302208772 1.474053972 1.578112084 1.339209435 1.70495215 1.576264899 1.558408773 1.74559023 1.227146853 1.573801985 1.724655461 1.568876157 1.584269369 0.751188741 1.71295662 1.551020031 1.584885097 1.950012084 1.77760811 2.241251653 1.297339899 1.917994203 1.87058311 1.491910097 1.519617879 2.003580461 2.123647514 1.214216555 2.228321355 1.801621521 1.377384601 1.186508773 2.023899501 2.028825329 1.635990561 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name S1P SPA Sph C8-cer C14-Cer C16-Cer C18:1-Cer C18-Cer C20-Cer C22-cer C24:1-Cer C24-Cer METABOLITES_END #END