#METABOLOMICS WORKBENCH hormel101_20161214_150201_mwtab.txt DATATRACK_ID:802 STUDY_ID:ST000527 ANALYSIS_ID:AN000805 PROJECT_ID:PR000387 VERSION 1 CREATED_ON January 5, 2017, 1:24 pm #PROJECT PR:PROJECT_TITLE Mayo Metabolomics Pilot and Feasibility Award: Effects of Curcumin PR:PROJECT_TITLE Supplementation on the Plasma Metabolome of Older Adults: Relation to Vascular PR:PROJECT_TITLE Function PR:PROJECT_SUMMARY Age is the major risk factor for cardiovascular diseases (CVD). Two key PR:PROJECT_SUMMARY contributors to the increased risk of CVD in middle-aged and older (MA/O) adults PR:PROJECT_SUMMARY are stiffening of the large elastic arteries and the development of vascular PR:PROJECT_SUMMARY endothelial dysfunction, indicated by impaired nitric oxide (NO)-induced PR:PROJECT_SUMMARY endothelium-dependent dilation (EDD). The mechanisms by which aging causes PR:PROJECT_SUMMARY arterial dysfunction are incompletely understood, but involve reductions in NO PR:PROJECT_SUMMARY bioavailability associated with the development of oxidative stress and PR:PROJECT_SUMMARY inflammation. Thus, establishing novel strategies to reduce arterial stiffness PR:PROJECT_SUMMARY and increase vascular endothelial function in MA/O adults by increasing NO PR:PROJECT_SUMMARY bioavailability and reducing oxidative stress and inflammation are a high PR:PROJECT_SUMMARY biomedical research priority. Curcumin is a naturally occurring phenol found in PR:PROJECT_SUMMARY the Indian spice turmeric that improves physiological function in animal models PR:PROJECT_SUMMARY of age-related diseases and is a promising nutraceutical for intervention for PR:PROJECT_SUMMARY promoting healthy aging. Our preclinical results indicate that chow supplemented PR:PROJECT_SUMMARY with curcumin reduces aortic pulse wave velocity (PWV), the most common and PR:PROJECT_SUMMARY clinically important measure of large elastic artery stiffness, restores PR:PROJECT_SUMMARY NO-mediated EDD and reduces arterial oxidative stress and inflammation in old PR:PROJECT_SUMMARY C57/BL6 mice. Preliminary data from our recently funded NIH R21 pilot grant PR:PROJECT_SUMMARY indicate that curcumin supplementation improves vascular function in humans. It PR:PROJECT_SUMMARY is possible that changes in the circulating (plasma) metabolome with oral PR:PROJECT_SUMMARY curcumin supplementation will provide insight into novel metabolic mechanisms by PR:PROJECT_SUMMARY which curcumin may improve vascular function. The goal of this project is to PR:PROJECT_SUMMARY identify key metabolic pathways that change with oral curcumin supplementation PR:PROJECT_SUMMARY and to relate those changes with improvements in vascular function in MA/O PR:PROJECT_SUMMARY adults with initial endothelial dysfunction. Metabolomic analysis of plasma PR:PROJECT_SUMMARY samples at baseline also may produce unique molecular signatures that predict PR:PROJECT_SUMMARY responsiveness (changes in vascular function) to curcumin supplementation among PR:PROJECT_SUMMARY individuals. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Seals PR:FIRST_NAME Douglas PR:ADDRESS Department of Integrative Physiology University of Colorado Boulder, CO 80309 PR:EMAIL seals@colorado.edu PR:PHONE 303-492-5305 #STUDY ST:STUDY_TITLE Effects of Curcumin Supplementation on the Acylcarnitine Concentration of Older ST:STUDY_TITLE Adults: Relation to Vascular Function ST:STUDY_SUMMARY Perform acylcarnitine concentrations metabolite analysis related to nitric oxide ST:STUDY_SUMMARY biology, oxidative stress and inflammation in plasma before and after 12 weeks ST:STUDY_SUMMARY of oral curcumin (2000 mg/d) or placebo (double-blind, randomized) in men and ST:STUDY_SUMMARY women aged 45-79 years who are free from clinical cardiovascular disease. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Seals ST:FIRST_NAME Douglas ST:ADDRESS Department of Integrative Physiology University of Colorado Boulder, CO 80309 ST:EMAIL seals@colorado.edu ST:PHONE 303-492-5305 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS IF131 ms5643-1 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU02 ms5643-2 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU02 ms5643-3 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU06 ms5643-4 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU06 ms5643-5 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS IF145 ms5643-6 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU10 ms5643-7 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU10 ms5643-8 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU11 ms5643-9 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU11 ms5643-10 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS IF119 ms5643-11 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU14 ms5643-12 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU14 ms5643-13 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU20 ms5643-14 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU20 ms5643-15 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9758 ms5643-16 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU22 ms5643-17 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU22 ms5643-18 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU23 ms5643-19 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU23 ms5643-20 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU24 ms5643-21 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU24 ms5643-22 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9737 ms5643-23 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU28 ms5643-24 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU28 ms5643-25 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU31 ms5643-26 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU31 ms5643-27 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU33 ms5643-28 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU33 ms5643-29 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 6218 ms5643-30 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU34 ms5643-31 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU34 ms5643-32 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU36 ms5643-33 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU36 ms5643-34 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU40 ms5643-35 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU40 ms5643-36 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS EC96 ms5643-37 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS 9732 ms5644-1 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU46 ms5644-2 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU46 ms5644-3 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU48 ms5644-4 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU48 ms5644-5 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9735 ms5644-6 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU49 ms5644-7 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU49 ms5644-8 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU51 ms5644-9 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU51 ms5644-10 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS 9760 ms5644-11 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU52 ms5644-12 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU52 ms5644-13 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU54 ms5644-14 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU54 ms5644-15 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9727 ms5644-16 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU56 ms5644-17 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU56 ms5644-18 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU57 ms5644-19 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU57 ms5644-20 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU58 ms5644-21 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU58 ms5644-22 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS IF152 ms5644-23 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU60 ms5644-24 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU60 ms5644-25 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU63 ms5644-26 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU63 ms5644-27 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS CU65 ms5644-28 group:Old-Curcumin | Time point:Pre SUBJECT_SAMPLE_FACTORS CU65 ms5644-29 group:Old-Curcumin | Time point:Post SUBJECT_SAMPLE_FACTORS 9759 ms5644-30 group:young | Time point:Baseline SUBJECT_SAMPLE_FACTORS CU66 ms5644-31 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU66 ms5644-32 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU71 ms5644-33 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU71 ms5644-34 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS CU75 ms5644-35 group:Old-Placebo | Time point:Pre SUBJECT_SAMPLE_FACTORS CU75 ms5644-36 group:Old-Placebo | Time point:Post SUBJECT_SAMPLE_FACTORS IF182 ms5644-37 group:young | Time point:Baseline #COLLECTION CO:COLLECTION_SUMMARY Healthy men & women aged 45-79 without clinical CVD, but with below normal CO:COLLECTION_SUMMARY baseline endothelial dysfunction (FMDBA < 7%). #TREATMENT TR:TREATMENT_SUMMARY "A 12-week randomized, double-blind, placebo controlled study will be conducted. TR:TREATMENT_SUMMARY After CTRC screening for inclusion/exclusion criteria, qualified subjects will TR:TREATMENT_SUMMARY be randomly assigned to 1 of 2 groups. The investigators involved in the TR:TREATMENT_SUMMARY acquisition and analysis of key outcomes will be blinded to the curcumin intake TR:TREATMENT_SUMMARY status of the subjects. With the assistance of dietary monitoring from the TR:TREATMENT_SUMMARY UC-Boulder CTRC bionutritionists, subjects will maintain their baseline diet TR:TREATMENT_SUMMARY with either unchanged (control) or enhanced curcumin intake delivered as TR:TREATMENT_SUMMARY capsules (Longvida®, Verdure Sciences): Group 1 = placebo (inert substances); TR:TREATMENT_SUMMARY Group 2 = curcumin (2000mg curcumin/day). Extensive published work has TR:TREATMENT_SUMMARY established that the curcumin dose of 2000 mg/day is well tolerated and safe. TR:TREATMENT_SUMMARY Sessions 1 & 2: Screening measurements. Session 3: Baseline measurements and TR:TREATMENT_SUMMARY blood draw. Sessions 4-8 (every other week to assess adherence and overall TR:TREATMENT_SUMMARY subject well-being): Body weight, BP, adherence, discuss any problems. Session TR:TREATMENT_SUMMARY 9: Identical to session 3 (stop intake of capsules after completion of TR:TREATMENT_SUMMARY post-testing)." #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Venous blood sampling. All blood samples are handled in a similar time frame and SP:SAMPLEPREP_SUMMARY immediately spun to extract plasma and stored in our -80 freezer until ready for SP:SAMPLEPREP_SUMMARY analysis. acylcarnitine concentrations in plasma. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C8 (150 x 2mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantiva QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples ms5643-1 ms5643-2 ms5643-3 ms5643-4 ms5643-5 ms5643-6 ms5643-7 ms5643-8 ms5643-9 ms5643-10 ms5643-11 ms5643-12 ms5643-13 ms5643-14 ms5643-15 ms5643-16 ms5643-17 ms5643-18 ms5643-19 ms5643-20 ms5643-21 ms5643-22 ms5643-23 ms5643-24 ms5643-25 ms5643-26 ms5643-27 ms5643-28 ms5643-29 ms5643-30 ms5643-31 ms5643-32 ms5643-33 ms5643-34 ms5643-35 ms5643-36 ms5643-37 ms5644-1 ms5644-2 ms5644-3 ms5644-4 ms5644-5 ms5644-6 ms5644-7 ms5644-8 ms5644-9 ms5644-10 ms5644-11 ms5644-12 ms5644-13 ms5644-14 ms5644-15 ms5644-16 ms5644-17 ms5644-18 ms5644-19 ms5644-20 ms5644-21 ms5644-22 ms5644-23 ms5644-24 ms5644-25 ms5644-26 ms5644-27 ms5644-28 ms5644-29 ms5644-30 ms5644-31 ms5644-32 ms5644-33 ms5644-34 ms5644-35 ms5644-36 ms5644-37 Factors group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:Old-Curcumin | Time point:Pre group:Old-Curcumin | Time point:Post group:young | Time point:Baseline group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:Old-Placebo | Time point:Pre group:Old-Placebo | Time point:Post group:young | Time point:Baseline Carnitine 44.72980602 49.50651059 55.3030726 52.30057258 60.68772139 25.69991129 64.11950446 50.79932258 48.94571306 56.04004988 36.29551052 28.67263445 42.65038865 35.59079151 43.31292378 33.89847332 36.27565928 37.29055391 46.57597131 47.91096719 45.05735147 50.13182464 39.97295269 28.21605593 28.21853734 37.19377912 38.33026259 36.96548986 37.50147333 26.74954559 35.28309729 42.32036179 41.62060559 43.45684527 51.45193208 39.86625227 29.80663652 39.39230392 46.33031222 57.16660773 43.18389072 42.38239691 51.95069448 39.49900434 41.93574402 68.01779167 60.32295486 48.87127091 61.55869453 59.4445375 39.97543409 42.40224815 51.27327093 66.83664291 79.90372149 45.38489693 58.02765526 49.44447546 54.33036185 31.67265306 30.82897537 31.9456076 31.6428762 40.89851674 45.88614073 44.59829155 45.34519445 45.39234114 54.28569656 51.93828746 33.46670885 46.79185355 41.66527088 91.01545295 Acetylcarnitine 10.47401038 15.37726661 16.97281001 13.58321081 14.94798355 9.322638478 24.858715 15.54352074 15.98024802 11.6799732 7.647690122 8.779210789 7.997568223 13.89834925 13.14152073 5.692343005 10.95044014 13.01000627 18.73957034 13.41447528 11.71223147 13.75939057 9.729588893 14.17130379 11.81148766 14.22837611 13.97030999 13.78916743 18.65023977 9.320157073 8.578216986 11.15391535 10.57822939 7.900793429 14.80158065 14.18371082 4.280423576 8.70973145 12.83878932 17.26561579 13.35244015 9.545964925 12.4765042 18.88845464 16.14650215 11.39957444 10.25812815 9.024869881 11.09684303 9.126607485 13.75939057 12.79412403 13.13407651 11.99511163 20.22593192 12.63283271 11.55094014 9.622888479 14.89339264 5.804006228 15.35989677 19.56835961 9.662590959 9.898324431 15.26808479 12.70479345 6.605500034 16.65767157 18.66264679 9.498818231 9.005018642 11.55590295 11.98270461 10.21346286 Propionylcarnitine 0.602981408 0.905712815 0.85360331 0.751865706 0.90323141 0.342433886 0.655090912 0.513650829 0.821345046 1.052115708 0.431764465 0.275435952 0.511169424 0.39454339 0.558316119 0.531020664 0.41439463 0.339952481 0.374692151 0.382136366 0.565760334 0.583130168 0.506206614 0.334989671 0.290324382 0.389580581 0.40943182 0.526057854 0.570723143 0.349878101 0.535983474 0.622832648 0.503725209 0.496280994 0.560797524 0.548390499 0.330026861 0.501243804 0.717126037 0.88338017 0.493799589 0.570723143 0.637721078 0.550871904 0.595537193 0.808938021 0.87841736 0.652609508 0.741940086 0.91315703 0.493799589 0.503725209 0.856084715 0.704719012 0.756828516 0.655090912 0.652609508 0.816382236 0.714644632 0.560797524 0.41439463 0.369729341 0.367247936 0.478911159 0.630276863 0.583130168 0.516132234 0.617869838 0.645165293 0.588092978 0.411913225 0.741940086 0.590574383 1.121595047 Butyrylcarnitine 0.126551654 0.168735538 0.205956613 0.203475208 0.218363637 0.076923554 0.156328513 0.129033059 0.151365703 0.191068183 0.079404959 0.173698348 0.233252067 0.129033059 0.173698348 0.106700414 0.270473142 0.275435952 0.119107439 0.119107439 0.131514463 0.148884298 0.151365703 0.091811984 0.096774794 0.129033059 0.131514463 0.109181819 0.126551654 0.081886364 0.116626034 0.141440083 0.143921488 0.161291323 0.158809918 0.143921488 0.071960744 0.141440083 0.171216943 0.186105373 0.138958678 0.143921488 0.171216943 0.188586778 0.176179753 0.173698348 0.168735538 0.481392564 0.223326447 0.196030993 0.163772728 0.151365703 0.193549588 0.263028927 0.171216943 0.158809918 0.143921488 0.186105373 0.200993803 0.138958678 0.193549588 0.213400828 0.126551654 0.151365703 0.198512398 0.178661158 0.200993803 0.181142563 0.205956613 0.153847108 0.133995868 0.163772728 0.143921488 0.305212811 Isovalerylcarnitine 0.136477273 0.158809918 0.178661158 0.243177687 0.210919423 0.116626034 0.215882233 0.143921488 0.166254133 0.156328513 0.186105373 0.126551654 0.178661158 0.101737604 0.126551654 0.148884298 0.133995868 0.124070249 0.186105373 0.166254133 0.151365703 0.146402893 0.200993803 0.094293389 0.096774794 0.163772728 0.158809918 0.168735538 0.235733472 0.148884298 0.161291323 0.193549588 0.158809918 0.153847108 0.173698348 0.176179753 0.138958678 0.124070249 0.181142563 0.263028927 0.143921488 0.166254133 0.230770662 0.161291323 0.240696282 0.238214877 0.186105373 0.213400828 0.258066117 0.171216943 0.191068183 0.173698348 0.191068183 0.141440083 0.163772728 0.138958678 0.143921488 0.235733472 0.240696282 0.138958678 0.121588844 0.094293389 0.111663224 0.121588844 0.198512398 0.168735538 0.181142563 0.166254133 0.168735538 0.171216943 0.141440083 0.196030993 0.173698348 0.248140497 Octanoylcarnitine 0.188586778 0.339952481 0.233252067 0.277917357 0.327545456 0.225807852 0.342433886 0.270473142 0.315138431 0.280398762 0.188586778 0.218363637 0.178661158 0.265510332 0.277917357 0.290324382 0.555834714 0.553353309 0.250621902 0.235733472 0.352359506 0.431764465 0.228289257 0.183623968 0.181142563 0.220845042 0.210919423 0.471466945 0.3995062 0.196030993 0.213400828 0.238214877 0.218363637 0.173698348 0.545909094 0.543427689 0.166254133 0.290324382 0.275435952 0.330026861 0.297768597 0.270473142 0.406950415 0.240696282 0.255584712 0.272954547 0.233252067 0.362285126 0.250621902 0.171216943 0.255584712 0.258066117 0.228289257 0.310175621 0.332508266 0.526057854 0.627795458 0.265510332 0.379654961 0.163772728 0.312657026 0.362285126 0.272954547 0.426801655 0.347396696 0.277917357 0.161291323 0.287842977 0.280398762 0.218363637 0.181142563 0.315138431 0.310175621 0.188586778 Lauroylcarnitine 0.052109504 0.109181819 0.062035124 0.074442149 0.116626034 0.052109504 0.101737604 0.084367769 0.069479339 0.054590909 0.059553719 0.059553719 0.042183885 0.069479339 0.059553719 0.059553719 0.151365703 0.146402893 0.106700414 0.079404959 0.069479339 0.121588844 0.054590909 0.057072314 0.049628099 0.052109504 0.054590909 0.091811984 0.091811984 0.044665289 0.03970248 0.044665289 0.054590909 0.03473967 0.079404959 0.074442149 0.03473967 0.089330579 0.084367769 0.099256199 0.089330579 0.062035124 0.176179753 0.074442149 0.062035124 0.079404959 0.057072314 0.089330579 0.062035124 0.042183885 0.066997934 0.074442149 0.054590909 0.086849174 0.086849174 0.133995868 0.153847108 0.084367769 0.138958678 0.032258265 0.091811984 0.086849174 0.052109504 0.106700414 0.057072314 0.054590909 0.03473967 0.094293389 0.091811984 0.042183885 0.032258265 0.109181819 0.101737604 0.044665289 Myristoylcarnitine 0.104219009 0.129033059 0.116626034 0.119107439 0.141440083 0.116626034 0.138958678 0.121588844 0.121588844 0.114144629 0.099256199 0.106700414 0.109181819 0.121588844 0.111663224 0.096774794 0.126551654 0.131514463 0.138958678 0.119107439 0.114144629 0.136477273 0.116626034 0.111663224 0.109181819 0.101737604 0.109181819 0.119107439 0.126551654 0.084367769 0.096774794 0.099256199 0.106700414 0.099256199 0.111663224 0.109181819 0.091811984 0.106700414 0.114144629 0.121588844 0.124070249 0.111663224 0.136477273 0.138958678 0.116626034 0.111663224 0.106700414 0.109181819 0.116626034 0.106700414 0.121588844 0.111663224 0.101737604 0.119107439 0.121588844 0.136477273 0.126551654 0.124070249 0.158809918 0.094293389 0.121588844 0.126551654 0.099256199 0.111663224 0.101737604 0.099256199 0.101737604 0.138958678 0.129033059 0.101737604 0.096774794 0.129033059 0.116626034 0.111663224 Palmitoylcarnitine 0.218363637 0.317619836 0.337471076 0.330026861 0.42432025 0.292805787 0.369729341 0.292805787 0.322582646 0.287842977 0.205956613 0.263028927 0.290324382 0.280398762 0.245659092 0.200993803 0.327545456 0.320101241 0.347396696 0.332508266 0.300250002 0.327545456 0.290324382 0.263028927 0.235733472 0.245659092 0.295287192 0.250621902 0.300250002 0.138958678 0.208438018 0.225807852 0.258066117 0.248140497 0.263028927 0.250621902 0.200993803 0.205956613 0.317619836 0.325064051 0.307694216 0.265510332 0.272954547 0.397024795 0.280398762 0.267991737 0.230770662 0.265510332 0.292805787 0.238214877 0.297768597 0.233252067 0.210919423 0.307694216 0.310175621 0.280398762 0.275435952 0.260547522 0.327545456 0.213400828 0.253103307 0.290324382 0.198512398 0.210919423 0.208438018 0.198512398 0.238214877 0.339952481 0.280398762 0.213400828 0.198512398 0.332508266 0.275435952 0.332508266 Oleoylcarnitine 0.339952481 0.493799589 0.466504135 0.595537193 0.697274797 0.397024795 0.590574383 0.46402273 0.339952481 0.325064051 0.275435952 0.379654961 0.382136366 0.40943182 0.352359506 0.235733472 0.560797524 0.622832648 0.45409711 0.392061985 0.431764465 0.349878101 0.411913225 0.42432025 0.498762399 0.347396696 0.392061985 0.377173556 0.431764465 0.263028927 0.315138431 0.357322316 0.401987605 0.315138431 0.42928306 0.357322316 0.315138431 0.263028927 0.3995062 0.42928306 0.466504135 0.379654961 0.255584712 0.642683888 0.431764465 0.384617771 0.275435952 0.327545456 0.367247936 0.287842977 0.377173556 0.290324382 0.270473142 0.416876035 0.543427689 0.382136366 0.43424587 0.426801655 0.625314053 0.275435952 0.535983474 0.652609508 0.347396696 0.39454339 0.4491343 0.367247936 0.225807852 0.506206614 0.451615705 0.263028927 0.267991737 0.295287192 0.312657026 0.374692151 Stearoylcarnitine 0.153847108 0.193549588 0.210919423 0.193549588 0.255584712 0.153847108 0.230770662 0.186105373 0.193549588 0.166254133 0.163772728 0.183623968 0.193549588 0.205956613 0.181142563 0.143921488 0.228289257 0.205956613 0.186105373 0.173698348 0.198512398 0.205956613 0.285361572 0.148884298 0.138958678 0.163772728 0.186105373 0.191068183 0.238214877 0.099256199 0.143921488 0.138958678 0.148884298 0.151365703 0.176179753 0.171216943 0.153847108 0.114144629 0.163772728 0.181142563 0.146402893 0.133995868 0.138958678 0.272954547 0.205956613 0.146402893 0.141440083 0.148884298 0.173698348 0.143921488 0.176179753 0.138958678 0.119107439 0.153847108 0.096774794 0.176179753 0.186105373 0.260547522 0.131514463 0.126551654 0.153847108 0.126551654 0.133995868 0.146402893 0.138958678 0.156328513 0.215882233 0.156328513 0.114144629 0.109181819 0.148884298 0.146402893 0.173698348 0.099256199 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Carnitine Acetylcarnitine Propionylcarnitine Butyrylcarnitine Isovalerylcarnitine Octanoylcarnitine Lauroylcarnitine Myristoylcarnitine Palmitoylcarnitine Oleoylcarnitine Stearoylcarnitine METABOLITES_END #END