#METABOLOMICS WORKBENCH araskind_20151211_9091981_mwtab.txt DATATRACK_ID:455 EX00366_CERAMIDES_EXCEL_REPORT.xlsx DATATRACK_ID:455 STUDY_ID:ST000562 ANALYSIS_ID:AN000864 VERSION 1 CREATED_ON 03-03-2022 #PROJECT PR:PROJECT_TITLE Ceramide assay PR:PROJECT_SUMMARY Ceramide metabolism in mouse adipose tissue PR:INSTITUTE University of Michigan PR:DEPARTMENT Internal Medicine PR:LAST_NAME Chen PR:FIRST_NAME Yuqing Eugene PR:ADDRESS Ann Arbor, MI 48105-5714 PR:EMAIL echenum@umich.edu PR:PHONE 734-936-9548 PR:DOI http://dx.doi.org/10.21228/M8P31H #STUDY ST:STUDY_TITLE Brown vs White adipose tissue ST:STUDY_SUMMARY Measure ceramide levels in brown and white adipose tissues of mice ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE 734-232-8175 ST:SUBMIT_DATE 2015-12-11 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0005184 S00016643 Adipose tissue:Brown | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005185 S00016644 Adipose tissue:Brown | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005186 S00016645 Adipose tissue:Brown | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005187 S00016646 Adipose tissue:Brown | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005188 S00016647 Adipose tissue:Brown | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005189 S00016648 Adipose tissue:Brown | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005190 S00016649 Adipose tissue:Brown | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005191 S00016650 Adipose tissue:Brown | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005192 S00016651 Adipose tissue:Brown | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005193 S00016652 Adipose tissue:Brown | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005194 S00016653 Adipose tissue:White | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005195 S00016654 Adipose tissue:White | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005196 S00016655 Adipose tissue:White | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005197 S00016656 Adipose tissue:White | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005198 S00016657 Adipose tissue:White | Treatment:N SUBJECT_SAMPLE_FACTORS SU0005199 S00016658 Adipose tissue:White | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005200 S00016659 Adipose tissue:White | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005201 S00016660 Adipose tissue:White | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005202 S00016661 Adipose tissue:White | Treatment:Y SUBJECT_SAMPLE_FACTORS SU0005203 S00016662 Adipose tissue:White | Treatment:Y #COLLECTION CO:COLLECTION_SUMMARY None CO:SAMPLE_TYPE Adipose Tissue #TREATMENT TR:TREATMENT_SUMMARY None #SAMPLEPREP SP:SAMPLEPREP_SUMMARY None SP:SAMPLEPREP_PROTOCOL_FILENAME Ceramides-20150305.docx #CHROMATOGRAPHY CH:METHODS_ID DQM0152 CH:METHODS_FILENAME QM033_2mmC18_Ceramide_MRM.m.zip CH:INSTRUMENT_NAME None CH:COLUMN_NAME Waters XBridge C18 (50 x 2.1mm, 3um) CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pg/mg tissue MS_METABOLITE_DATA_START Samples S00016643 S00016644 S00016645 S00016646 S00016647 S00016648 S00016649 S00016650 S00016651 S00016652 S00016653 S00016654 S00016655 S00016656 S00016657 S00016658 S00016659 S00016660 S00016661 S00016662 Factors Adipose tissue:Brown | Treatment:N Adipose tissue:Brown | Treatment:N Adipose tissue:Brown | Treatment:N Adipose tissue:Brown | Treatment:N Adipose tissue:Brown | Treatment:N Adipose tissue:Brown | Treatment:Y Adipose tissue:Brown | Treatment:Y Adipose tissue:Brown | Treatment:Y Adipose tissue:Brown | Treatment:Y Adipose tissue:Brown | Treatment:Y Adipose tissue:White | Treatment:N Adipose tissue:White | Treatment:N Adipose tissue:White | Treatment:N Adipose tissue:White | Treatment:N Adipose tissue:White | Treatment:N Adipose tissue:White | Treatment:Y Adipose tissue:White | Treatment:Y Adipose tissue:White | Treatment:Y Adipose tissue:White | Treatment:Y Adipose tissue:White | Treatment:Y Cer C14 138.4540 115.8590 159.9650 305.9430 110.3710 191.6480 175.9660 150.3350 135.3080 109.0780 52.9660 54.6370 86.4950 88.1110 80.4430 97.6860 82.3790 74.1090 110.3810 84.9350 Cer C16 18811.8650 13524.4440 19147.4820 28850.8940 13583.9210 18273.5320 19607.2330 18574.2120 20493.1620 13930.7080 6517.1970 5389.8590 8381.8710 8199.8790 7987.7390 8549.5620 7384.3810 6642.2640 10791.8900 12396.9900 Cer C18 2232.7380 3408.1860 3083.7150 3361.4690 2083.2220 5161.9000 4508.7480 3557.2770 3186.0230 2228.8670 612.8930 569.3570 1007.3100 760.1410 1051.8320 1403.1040 883.8920 895.5050 1476.3160 1058.3090 Cer C20 4665.8320 5612.1020 5996.2210 7013.9040 6597.6590 8601.5700 6709.3890 5322.9520 4632.8600 4994.2410 1152.8650 931.2510 1452.3290 1437.5290 1510.5080 1430.1970 1094.9240 1184.3570 1796.0060 2065.7630 Cer C22 8368.4200 11387.1480 8388.7790 10643.8570 12445.2220 9585.9050 8344.8170 7814.5880 6200.9700 8674.3000 1380.1700 1198.0980 1922.9980 1749.0670 1733.6120 1778.1390 1746.4610 1724.2500 2068.6480 2286.9940 Cer C24 16945.8810 14887.3200 15668.7500 19036.9030 14231.5070 17716.2380 18090.7830 17851.1920 16906.8430 13231.7810 5128.4650 3882.1030 7254.1990 7289.0460 7042.9040 5724.2810 7088.1970 6958.1380 7325.1240 7509.6750 Cer C24:1 37195.1800 24523.0180 48497.1260 31851.9880 22672.3060 45574.0550 48106.5500 42916.2360 42109.8290 29122.5280 15400.7930 11022.8170 17991.9310 26183.8040 19881.2460 14307.8810 14831.2390 14960.3720 19620.1390 24761.3320 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant Cer C14 5282310 Cer C16 5283564 Cer C18 5283565 Cer C20 5283566 Cer C22 5283567 Cer C24 5283571 Cer C24:1 5283568 METABOLITES_END #END