#METABOLOMICS WORKBENCH sbhattacharya_20170403_135141_mwtab.txt DATATRACK_ID:875 STUDY_ID:ST000579 ANALYSIS_ID:AN000890 PROJECT_ID:PR000425 VERSION 1 CREATED_ON April 5, 2017, 5:51 pm #PROJECT PR:PROJECT_TITLE Phosphatidylethanolamine Analysis in Aqueous Humor and Trabecular Meshwork of PR:PROJECT_TITLE DBA/2J mice PR:PROJECT_SUMMARY Compared Phosphatidylethanolamine (PE) profiles within aqueous humor (AH) and PR:PROJECT_SUMMARY trabecular meshwork (TM) of normotensive and hypertensive DBA/2J mice using PR:PROJECT_SUMMARY direct infusion triple quadrupole mass spectrometry. PR:INSTITUTE University of Miami PR:LAST_NAME Bhattacharya PR:FIRST_NAME Sanjoy PR:ADDRESS McKnight Vision Research Building, Bascom Palmer Eye Institute, University of PR:ADDRESS Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida PR:ADDRESS 33136 PR:EMAIL Sbhattacharya@med.miami.edu PR:PHONE 305-482-4103 #STUDY ST:STUDY_TITLE DBA2J Phosphatidylethanolamine Analysis ST:STUDY_SUMMARY AH and TM samples were obtained from young-normotensive DBA/2J (3 and 7.5 ST:STUDY_SUMMARY months) and old-hypertensive DBA/2J mice (8-8.5, 10 and 12 month). Lipids were ST:STUDY_SUMMARY extracted using modified Bligh and Dyer method and subjected to mass ST:STUDY_SUMMARY spectrometric identification using appropriate class-specific lipid standards ST:STUDY_SUMMARY and ratiometric quantification. Corresponding aqueous phase (of extraction) ST:STUDY_SUMMARY protein concentrations were measured using Bradford method. ST:INSTITUTE University of Miami ST:LAST_NAME Bhattacharya ST:FIRST_NAME Sanjoy ST:ADDRESS McKnight Vision Research Building, Bascom Palmer Eye Institute, University of ST:ADDRESS Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida ST:ADDRESS 33136 ST:EMAIL SBhattacharya@med.miami.edu ST:PHONE 305-482-4103 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN DBA/2J #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - DBA2J 3M MTML4 Tissue Location:TM | Sex:Male | Phenotype:Normotensive Age (months)=3; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 7.5M MTM2L3 Tissue Location:TM | Sex:Male | Phenotype:Normotensive Age (months)=7.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 8-8.5M MTM4L5 Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Age (months)=8-8.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 10M MTML4 Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Age (months)=10; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 12M MTML2 Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Age (months)=12; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 3M MAHL2 Tissue Location:AH | Sex:Male | Phenotype:Normotensive Age (months)=3; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 7.5M MAH2L2 Tissue Location:AH | Sex:Male | Phenotype:Normotensive Age (months)=7.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 8-8.5M MAH4L5 Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Age (months)=8-8.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 10M MAHL5 Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Age (months)=10; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 12M MAHL2 Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Age (months)=12; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 3M FTML3 Tissue Location:TM | Sex:Female | Phenotype:Normotensive Age (months)=3; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 7.5M FTML2 Tissue Location:TM | Sex:Female | Phenotype:Normotensive Age (months)=7.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 8-8.5M FTM4L5 Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Age (months)=8-8.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 10M FTML5 Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Age (months)=10; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 12M FTML2 Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Age (months)=12; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 3M FAH Tissue Location:AH | Sex:Female | Phenotype:Normotensive Age (months)=3; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 7.5M FAHL2 Tissue Location:AH | Sex:Female | Phenotype:Normotensive Age (months)=7.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 8-8.5M FAH4L5 Tissue Location:AH | Sex:Female | Phenotype:Hypertensive Age (months)=8-8.5; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 10M FAHL5 Tissue Location:AH | Sex:Female | Phenotype:Hypertensive Age (months)=10; Lipid Class=PE SUBJECT_SAMPLE_FACTORS - DBA2J 12M FAHL2 Tissue Location:AH | Sex:Female | Phenotype:Hypertensive Age (months)=12; Lipid Class=PE #COLLECTION CO:COLLECTION_SUMMARY DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of CO:COLLECTION_SUMMARY Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution CO:COLLECTION_SUMMARY (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to CO:COLLECTION_SUMMARY AH procurement. Normotensive and hypertensive AH was obtained from the anterior CO:COLLECTION_SUMMARY chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 CO:COLLECTION_SUMMARY Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog CO:COLLECTION_SUMMARY no. 7803-05 Hamilton). TM samples were obtained through surgical removal after CO:COLLECTION_SUMMARY euthanization with carbon dioxide and adhering to animal procedures approved by CO:COLLECTION_SUMMARY the IACUC. #TREATMENT TR:TREATMENT_SUMMARY No treatment was performed on the mice. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for SP:SAMPLEPREP_SUMMARY phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. SP:SAMPLEPREP_SUMMARY Phospholipids extracted in the lower organic phase were dried using a Speed-Vac SP:SAMPLEPREP_SUMMARY (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for SP:SAMPLEPREP_SUMMARY oxidation prevention. Proteins procured from the upper aqueous phase were SP:SAMPLEPREP_SUMMARY quantified using a suitable modification of Bradford’s method. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME TSQ Quantum Access Max CH:COLUMN_NAME None #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantiva Access Max MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/ ug of protein MS_METABOLITE_DATA_START Samples DBA2J 3M MTML4 DBA2J 7.5M MTM2L3 DBA2J 8-8.5M MTM4L5 DBA2J 10M MTML4 DBA2J 12M MTML2 DBA2J 3M MAHL2 DBA2J 7.5M MAH2L2 DBA2J 8-8.5M MAH4L5 DBA2J 10M MAHL5 DBA2J 12M MAHL2 DBA2J 3M FTML3 DBA2J 7.5M FTML2 DBA2J 8-8.5M FTM4L5 DBA2J 10M FTML5 DBA2J 12M FTML2 DBA2J 3M FAH DBA2J 7.5M FAHL2 DBA2J 8-8.5M FAH4L5 DBA2J 10M FAHL5 DBA2J 12M FAHL2 Factors Tissue Location:TM | Sex:Male | Phenotype:Normotensive Tissue Location:TM | Sex:Male | Phenotype:Normotensive Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Tissue Location:TM | Sex:Male | Phenotype:Hypertensive Tissue Location:AH | Sex:Male | Phenotype:Normotensive Tissue Location:AH | Sex:Male | Phenotype:Normotensive Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Tissue Location:AH | Sex:Male | Phenotype:Hypertensive Tissue Location:TM | Sex:Female | Phenotype:Normotensive Tissue Location:TM | Sex:Female | Phenotype:Normotensive Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Tissue Location:TM | Sex:Female | Phenotype:Hypertensive Tissue Location:AH | Sex:Female | Phenotype:Normotensive Tissue Location:AH | Sex:Female | Phenotype:Normotensive Tissue Location:AH | Sex:Female | Phenotype:Hypertensive Tissue Location:AH | Sex:Female | Phenotype:Hypertensive Tissue Location:AH | Sex:Female | Phenotype:Hypertensive NAPE(18:1(9Z)/16:1(9Z)/18:0) 3.831681224 11.42627298 3.723704086 71.92525965 0.010234058 2.962719983 2.336748931 111.3677584 1.585185926 PE(12:0/19:0) 0.034485107 1.220833135 PE(13:0/22:4(7Z,10Z,13Z,16Z)) 5.133245753 3.263749258 5.06627536 5.784177134 20.92784262 8.670055527 10.13545075 5.913976211 17.1049645 PE(16:0/20:4(5Z,8Z,11Z,14Z)) 3.78280266 1.596191077 0.471470357 8.84945492 3.679495284 0.868043942 PE(16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 1.282541093 9.534291465 26.68132652 5.968349589 55.24315111 29.04663011 2.480538547 5.118097631 6.084285401 132.4243569 2.655568857 5.145217983 PE(17:0/20:0) 38.94563114 15.60949603 71.01806404 15.63615236 62.33370646 0.29303557 2.979253414 47.79647349 18.01316303 12.4212457 8.366031113 12.24431514 415.2324256 1.33770552 8.591422586 PE(17:0/20:4(5Z,8Z,11Z,14Z)) 11.4845239 7.66774301 114.8618246 17.38283199 367.8095802 2.620354385 54.59322562 5.795204561 4.911986628 5.449752404 16.17268022 36.47969453 3.9056655 29.83044581 141.2167424 109.2196104 3.862102455 PE(17:0/22:1(11Z)) 3.725727626 4.130720437 24.58236503 248.7621088 PE(17:0/22:2(13Z,16Z)) 8.537266676 6.399149334 4.747018548 121.1463666 6.563957056 8.698536209 PE(17:0/22:4(7Z,10Z,13Z,16Z)) 14.10977607 16.36235321 53.86613824 7.979202193 12.57975401 9.170425728 35.05179393 48.10650821 10.21435077 19.38699614 430.3076298 2.305853219 PE(18:0/18:0) 8.087006049 6.132881277 16.10321584 65.7466774 0.049149683 0.703153056 2.345584327 11.68592837 6.146450662 123.7264529 6.917365676 PE(18:0/18:1(9Z)) 4.50418129 14.23841973 60.19223005 0.212812647 31.93338384 13.72739714 8.29849311 30.62872433 4.961593923 PE(18:0/20:0) 1.211384717 9.176846995 22.88121972 2.044846624 39.94485489 88.0518003 1.027402351 80.01764724 1.487219459 15.07790119 20.8839875 24.13811369 2.677896107 15.60546346 44.65837678 144.2832358 1.652475133 PE(18:0/22:0) 19.79254023 10.03763136 31.59582579 12.63034548 0.285261273 31.720915 9.742099673 7.30466148 10.48917894 8.915670701 25.3279353 82.61201766 357.6072794 3.054099054 3.009173216 PE(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 26.48007906 21.00787832 14.07102156 16.46965051 11.87902118 0.582467055 34.3957693 21.12473429 23.94306163 9.563644221 22.02704755 101.7598766 0.515812835 PE(18:1(9E)/18:1(9E)) 0.138200414 4.108480332 6.494435242 13.44220761 PE(19:0/16:0) 1.12529498 2.003367395 12.79467395 3.496533817 PE(20:0/18:4(6Z,9Z,12Z,15Z)) 10.0246643 6.402612913 7.347356922 29.5914573 64.41843284 43.95169184 3.67889647 2.871398917 41.97943935 6.935279595 42.56893943 6.245428545 27.25902509 865.0038133 0.551264915 5.854179698 PE(20:0/20:2(11Z,14Z)) 8.514990109 4.80633712 44.23470215 26.0644205 19.98313897 144.5519486 1.160625729 140.5836461 7.234924014 9.399162999 27.4298843 19.3488404 11.14528421 32.35037486 14.20063656 323.116048 271.1085699 3.563584042 9.685377637 PE(20:0/22:0) 3.310598079 4.372591397 99.81277126 14.78530864 7.814977557 5.766386459 5.754229267 108.6153937 0.318051232 PE(20:0/22:4(7Z,10Z,13Z,16Z)) 19.06916351 25.09486834 6.616766736 5.591452263 4.192904924 22.50261748 1.141183045 53.77623003 3.874238604 4.542791526 14.07900706 8.16465989 5.39187556 18.65570563 89.11425408 1.99497164 2.541754753 PE(20:2(5Z,8Z)/18:0) 15.07209569 5.426650549 56.39353235 2.317772942 10.47089299 8.723392874 61.26081473 1.87400441 12.70786402 39.7711408 0.546752265 0.305981439 PE(20:5(5Z,8Z,11Z,14Z,17Z)/22:5(7Z,10Z,13Z,16Z,19Z)) 19.56221555 26.17418287 17.99377295 19.74960713 2.783943975 189.8037169 3.802281552 127.7577578 14.77008865 4.345923255 25.75448971 14.31807348 96.97784584 40.4230754 11.62098901 347.6095843 559.1645712 3.628589898 0.778549365 PE(21:0/22:0) 3.127071246 12.62701358 76.65975539 118.5516457 0.618672731 4.929138835 19.75247295 4.317904198 14.11541135 13.51163752 157.8521728 265.9864958 1.611937726 PE(21:0/22:1(11Z)) 10.97876136 6.86666731 10.11115389 37.05282957 0.822006743 0.869827163 5.759016996 5.302689892 4.978003501 5.419263627 PE(21:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 14.74861523 8.338335632 17.14159564 17.11862245 5.073559799 74.44309297 2.708364136 53.21829386 16.2653918 23.67588555 5.855960484 42.6731317 15.45210138 23.17416047 247.4790021 6.814871249 PE(22:0/22:2(13Z,16Z)) 3.485273753 5.060658221 12.97696514 106.4013921 80.75819839 0.278303215 0.132210412 3.455863892 2.804020452 PE(22:0/22:4(7Z,10Z,13Z,16Z)) 1.019681585 6.927972169 5.365259295 13.36765989 86.28761789 6.588288716 2.121718359 10.8061108 10.30124855 103.2986139 0.896931959 0.100929651 PE(22:0/24:1(15Z)) 13.55912903 5.159259631 14.9282222 151.1241589 10.24260491 5.22399793 1.464903686 7.307190353 3.795553886 89.53216 6.529671587 PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 6.285529519 4.651303727 189.7200697 12.99920328 PE(24:0/20:0) 10.49396212 12.22362061 2.00039911 10.00195854 156.5206161 2.072469502 61.5488146 5.82513686 26.90244311 7.097018293 28.89559426 4.634334893 13.54281274 0.556485298 PE(26:2(5Z,9Z)/26:2(5Z,9Z)) 2.677596042 6.732184806 4.944417877 7.922522344 121.1103897 8.847154347 10.64906891 30.65260276 138.5036251 PE-NMe2(16:0/16:0) 1.203330707 2.357445661 12.47887783 1.460806159 0.106378557 21.65727334 3.634133844 4.897854885 4.184227293 PE(13:0/20:1(11Z)) 1.973996463 10.3596172 1.112413266 0.137372073 PE(15:0/15:0) 2.768105981 0.324148301 7.163356676 PE(16:1(9Z)/0:0) 0.067767415 PE(16:1(9Z)/16:1(9Z)) 0.410787359 0.025043682 2.779516167 3.255378171 PE(17:0/14:1(9Z)) 1.209803679 4.314608881 1.377599107 0.595560564 PE(17:0/22:0) 0.111551941 0.377258272 7.498351792 9.147322622 48.80476226 9.983450765 4.370840832 17.52547648 0.858553624 PE(18:0/10:0) 0.555385255 2.410734389 0.075924242 PE(18:1(9Z)/18:2(9Z,12Z)) 3.122698614 9.28338756 17.90618597 0.810310485 PE(18:1(9Z)/22:2(13Z,16Z)) 9.88029907 1.254482403 35.0173198 18.75425802 12.5916427 5.980599767 8.20274099 145.129412 0.898589411 PE(19:0/22:4(7Z,10Z,13Z,16Z)) 6.375199567 1.484421286 116.3981972 1.308372418 14.69732768 10.52021561 17.81524145 1.709226606 PE(20:0/22:1(13Z)) 6.888525712 6.051273772 8.760733485 7.093011799 16.7590702 0.472828851 64.55047713 731.4321303 PE(20:0/24:1(15Z)) 1.115111994 9.132189549 3.241513385 12.07483691 3.229099274 1.022891897 PE(21:0/22:2(13Z,16Z)) 7.647995838 17.0136583 13.2655503 1.854252898 6.493098977 113.7929198 0.620250921 PE-NMe(11:0/11:0) 0.363215143 0.340789285 1.037011735 PE-NMe(16:0/16:0) 1.753755827 3.950748344 2.493575924 1.366473561 PE-NMe(16:0/18:1(9Z)) 6.812684656 8.412210837 1.206190649 16.5422424 13.38047292 0.378441695 PE-NMe(20:0/20:0) 7.569261978 3.244101555 16.81583651 191.5850474 2.310265184 76.49998375 9.096159233 8.846503151 17.24968379 17.27921106 11.40224205 10.8936504 142.9293377 2.604364781 5.47197791 PE(12:0/17:1(9Z)) 7.339245226 PE(20:0/22:2(13Z,16Z)) 1.023550733 1.064781544 1.505515374 8.707274193 1.590221605 27.9998594 2.145251708 PE-NMe(18:1(9E)/18:1(9E)) 2.950198534 1.390923292 163.8022672 0.79361035 2.063927303 4.772007208 8.55090467 34.16657998 10.17221582 12.08323599 1.843866726 PE(12:0/13:0) 0.523415074 PE(12:0/15:0) 2.260207906 0.417606733 PE(12:0/18:2(9Z,12Z)) 1.505221494 2.894093748 PE(12:0/20:4(5Z,8Z,11Z,14Z)) 0.036025448 PE(12:0/22:4(7Z,10Z,13Z,16Z)) 1.566175639 4.269645032 PE(13:0/20:2(11Z,14Z)) 2.053253202 8.531881098 PE(13:0/20:4(5Z,8Z,11Z,14Z)) 0.569314373 5.736844211 PE(13:0/22:2(13Z,16Z)) 1.844587974 1.107133445 PE(16:0/16:0) 0.254170635 0.082638177 0.581634576 2.222752096 1.846098911 PE(16:0/18:1(9Z)) 3.018618225 12.59866329 5.420657839 1.52252352 0.284797326 PE(16:0/18:3(9Z,12Z,15Z)) 2.978515363 2.663130295 1.957893014 4.825410284 PE(12:0/18:4(6Z,9Z,12Z,15Z)) 111.6699031 PE(13:0/20:3(8Z,11Z,14Z)) 0.558200579 PE(20:4(5Z,8Z,11Z,14Z)/0:0) 15.3884888 1.727223958 PE(12:0/17:2(9Z,12Z)) 0.131618258 PE(20:0/0:0) 4.45927051 2.065857331 PE(12:0/12:0) 1.273155708 PE(12:0/15:1(9Z)) 0.879017175 0.169070745 PE(12:0/18:3(6Z,9Z,12Z)) 3.938305808 PE(16:0/0:0) 2.992756467 0.935283376 PE(18:0/0:0) 1.767964773 PE(18:2(9Z,12Z)/0:0) 3.328109667 14.19869127 PE(22:1(11Z)/0:0) 4.070762978 PE(22:4(7Z,10Z,13Z,16Z)/0:0) 0.674688343 PE(8:0/8:0) 5.617431797 PE(12:0/14:0) 4.358602691 PE(14:1(9Z)/14:1(9Z)) 1.564740163 PE(20:1(11Z)/0:0) 0.336941373 PE(12:0/14:1(9Z)) 2.044942774 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID NAPE(18:1(9Z)/16:1(9Z)/18:0) 980.7662 PE(12:0/12:0) 578.38 PE(12:0/13:0) 592.3957 PE(12:0/14:0) 606.4113 PE(12:0/14:1(9Z)) 604.3957 PE(12:0/15:0) 620.427 PE(12:0/15:1(9Z)) 618.4113 PE(12:0/17:1(9Z)) 646.4426 PE(12:0/17:2(9Z,12Z)) 644.427 PE(12:0/18:2(9Z,12Z)) 658.4426 PE(12:0/18:3(6Z,9Z,12Z)) 656.427 PE(12:0/18:4(6Z,9Z,12Z,15Z)) 654.4113 PE(12:0/19:0) 676.4896 PE(12:0/20:4(5Z,8Z,11Z,14Z)) 682.4426 PE(12:0/22:4(7Z,10Z,13Z,16Z)) 710.4739 PE(13:0/20:1(11Z)) 702.5052 PE(13:0/20:2(11Z,14Z)) 700.4896 PE(13:0/20:3(8Z,11Z,14Z)) 698.4739 PE(13:0/20:4(5Z,8Z,11Z,14Z)) 696.4583 PE(13:0/22:2(13Z,16Z)) 728.5209 PE(13:0/22:4(7Z,10Z,13Z,16Z)) 724.4896 PE(14:1(9Z)/14:1(9Z)) 630.4113 PE(15:0/15:0) 662.4739 PE(16:0/0:0) 452.2755 PE(16:0/16:0) 690.5052 PE(16:0/18:1(9Z)) 716.5209 PE(16:0/18:3(9Z,12Z,15Z)) 712.4896 PE(16:0/20:4(5Z,8Z,11Z,14Z)) 738.5052 PE(16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 762.5052 PE(16:1(9Z)/0:0) 450.2599 PE(16:1(9Z)/16:1(9Z)) 686.4739 PE(17:0/14:1(9Z)) 674.4739 PE(17:0/20:0) 760.5835 PE(17:0/20:4(5Z,8Z,11Z,14Z)) 752.5209 PE(17:0/22:0) 788.6148 PE(17:0/22:1(11Z)) 786.5991 PE(17:0/22:2(13Z,16Z)) 784.5835 PE(17:0/22:4(7Z,10Z,13Z,16Z)) 780.5522 PE(18:0/0:0) 480.3068 PE(18:0/10:0) 634.4426 PE(18:0/18:0) 746.5678 PE(18:0/18:1(9Z)) 744.5522 PE(18:0/20:0) 774.5991 PE(18:0/22:0) 802.6304 PE(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 790.5365 PE(18:1(9E)/18:1(9E)) 742.5365 PE(18:1(9Z)/18:2(9Z,12Z)) 740.5209 PE(18:1(9Z)/22:2(13Z,16Z)) 796.5835 PE(18:2(9Z,12Z)/0:0) 476.2755 PE(19:0/16:0) 732.5522 PE(19:0/22:4(7Z,10Z,13Z,16Z)) 808.5835 PE(20:0/0:0) 508.3381 PE(20:0/18:4(6Z,9Z,12Z,15Z)) 766.5365 PE(20:0/20:2(11Z,14Z)) 798.5991 PE(20:0/22:0) 830.6617 PE(20:0/22:1(13Z)) 828.6461 PE(20:0/22:2(13Z,16Z)) 826.6304 PE(20:0/22:4(7Z,10Z,13Z,16Z)) 822.5991 PE(20:0/24:1(15Z)) 856.6774 PE(20:1(11Z)/0:0) 506.3225 PE(20:2(5Z,8Z)/18:0) 770.5678 PE(20:4(5Z,8Z,11Z,14Z)/0:0) 500.2755 PE(20:5(5Z,8Z,11Z,14Z,17Z)/22:5(7Z,10Z,13Z,16Z,19Z)) 810.5052 PE(21:0/22:0) 844.6774 PE(21:0/22:1(11Z)) 842.6617 PE(21:0/22:2(13Z,16Z)) 840.6461 PE(21:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 832.5835 PE(22:0/22:2(13Z,16Z)) 854.6617 PE(22:0/22:4(7Z,10Z,13Z,16Z)) 850.6304 PE(22:0/24:1(15Z)) 884.7087 PE(22:1(11Z)/0:0) 534.3538 PE(22:4(7Z,10Z,13Z,16Z)/0:0) 528.3068 PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 834.5052 PE(24:0/20:0) 858.693 PE(26:2(5Z,9Z)/26:2(5Z,9Z)) 962.7556 PE(8:0/8:0) 466.2548 PE-NMe(11:0/11:0) 564.3644 PE-NMe(16:0/16:0) 704.5209 PE-NMe(16:0/18:1(9Z)) 730.5365 PE-NMe(18:1(9E)/18:1(9E)) 756.5522 PE-NMe(20:0/20:0) 816.6461 PE-NMe2(16:0/16:0) 718.5365 METABOLITES_END #END