#METABOLOMICS WORKBENCH hormel101_20170623_124631 DATATRACK_ID:1091 STUDY_ID:ST000642 ANALYSIS_ID:AN000974 PROJECT_ID:PR000459 VERSION 1 CREATED_ON June 25, 2017, 5:03 pm #PROJECT PR:PROJECT_TITLE Role of the Serine Biosynthesis Pathway in Supporting the Warburg Effect of PR:PROJECT_TITLE Pancreatic Cancer Cells PR:PROJECT_SUMMARY Pancreatic cancer cells metabolize glucose differently than normal adult cells, PR:PROJECT_SUMMARY relying on aerobic glycolysis even in oxygen-rich environments. This phenomenon, PR:PROJECT_SUMMARY known as the Warburg effect, is the basis of PET scans for tumor imaging and PR:PROJECT_SUMMARY diagnosis, but a definitive explanation for how this benefits cancer cells has PR:PROJECT_SUMMARY remained elusive, and altered cell metabolism has not been fully exploited for PR:PROJECT_SUMMARY therapeutic benefit. The Warburg effect is accompanied by expression of the M2 PR:PROJECT_SUMMARY isoform of pyruvate kinase (PK); while many differentiated normal cells express PR:PROJECT_SUMMARY PKM1, proliferating cells, including all cancer cells, express PKM1. We have PR:PROJECT_SUMMARY generated both normal cell lines and pancreatic cancer cell lines that can be PR:PROJECT_SUMMARY genetically controlled to express either PKM1 or PKM2. While normal PR:PROJECT_SUMMARY proliferating cells stop proliferating when forced to express PKM1 rather than PR:PROJECT_SUMMARY PKM2, pancreatic cancer cells proliferate just as rapidly with forced PKM1 PR:PROJECT_SUMMARY expression. Preliminary data shows that pancreatic cancer cells upregulate the PR:PROJECT_SUMMARY serine biosynthesis pathway during forced PKM1 expression. To probe the role of PR:PROJECT_SUMMARY the serine biosynthesis pathway in supporting cancer proliferation in the PR:PROJECT_SUMMARY context of isoform-specific PK expression, we have targeted genes in the serine PR:PROJECT_SUMMARY biosynthesis pathway using the CRISPR/Cas9 system and generated pancreatic PR:PROJECT_SUMMARY cancer knockout cell lines. The proposed research will use isotope-labeled PR:PROJECT_SUMMARY precursors and genetic engineering to identify the metabolic dependencies of PR:PROJECT_SUMMARY pancreatic cancer cells. Genetically engineered pancreatic cancer cell lines PR:PROJECT_SUMMARY cultured with 13C-glucose, 13C-glutamine, or 13C-serine will be extracted and PR:PROJECT_SUMMARY sent to the Mayo Clinic Metabolomics Resource Core for isotopic enrichment PR:PROJECT_SUMMARY analysis of various amino acids, TCA cycle metabolites, fatty acids, and PR:PROJECT_SUMMARY sphingolipids. This work will provide crucial first insight for altered PR:PROJECT_SUMMARY metabolism of pancreatic cancer cells that can lead to novel metabolic targets PR:PROJECT_SUMMARY for effectively treating pancreatic cancer. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Lunt PR:FIRST_NAME Sophia PR:ADDRESS Michigan State University 410B Biochemistry Building 603 Wilson Road East PR:ADDRESS Lansing, MI 48824 PR:EMAIL sophia@msu.edu PR:PHONE 517-432-4886 #STUDY ST:STUDY_TITLE Trace 13C-glucose, 13C-glutamine, and 13C-serine in genetically engineered ST:STUDY_TITLE pancreatic cell lines through free fatty acids (part I) ST:STUDY_SUMMARY Trace 13C-glucose, 13C-glutamine, and 13C-serine in genetically engineered ST:STUDY_SUMMARY pancreatic cell lines through free fatty acids ST:INSTITUTE Mayo Clinic ST:LAST_NAME Lunt ST:FIRST_NAME Sophia ST:ADDRESS Michigan State University 410B Biochemistry Building 603 Wilson Road East ST:ADDRESS Lansing, MI 48824 ST:EMAIL sophia@msu.edu ST:PHONE 517-432-4886 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - ms5770-1 experiment:20_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-2 experiment:21_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-3 experiment:22_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-4 experiment:23_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-5 experiment:24_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-6 experiment:25_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-7 experiment:26_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-8 experiment:27_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-9 experiment:28_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-10 experiment:29_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-11 experiment:30_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-12 experiment:31_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-13 experiment:32_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-14 experiment:33_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-15 experiment:34_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-16 experiment:35_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-17 experiment:36_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH SUBJECT_SAMPLE_FACTORS - ms5770-18 experiment:37_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 groupID=PSPH #COLLECTION CO:COLLECTION_SUMMARY Pancreatic ductal adenocarcinoma cell line with PSPH KO. These cells have been CO:COLLECTION_SUMMARY incubated with no label, or 13C-glucose label for 24hours or 48 hours. #TREATMENT TR:TREATMENT_SUMMARY Pancreatic cancer cells exhibit altered metabolism, which is mediated in part by TR:TREATMENT_SUMMARY expressing the proliferation-supportive M2 isoform of pyruvate kinase (PK). TR:TREATMENT_SUMMARY Unlike normal embryonic cells that stop proliferating when forced to express the TR:TREATMENT_SUMMARY proliferation-incompatible M1 isoform of PK, pancreatic cancer cells can TR:TREATMENT_SUMMARY proliferate just as rapidly with either the M1 or M2 isoform. During forced PKM1 TR:TREATMENT_SUMMARY expression, pancreatic cancer cells upregulate their serine biosynthesis TR:TREATMENT_SUMMARY pathway. The three enzymes in the serine biosynthesis pathway include TR:TREATMENT_SUMMARY phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase (PSAT), TR:TREATMENT_SUMMARY and phosphoserine phosphatase (PSPH). Each of the genes encoding the three TR:TREATMENT_SUMMARY enzymes in the serine biosynthesis pathway have successfully been knocked out TR:TREATMENT_SUMMARY from pancreatic cancer cells using the CRISPR/Cas9 system in our laboratory, TR:TREATMENT_SUMMARY with multiple confirmed clones for each knockout ready for metabolic TR:TREATMENT_SUMMARY characterization. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY In this proposal, we will interrogate the metabolic pathways that support SP:SAMPLEPREP_SUMMARY pancreatic cancer proliferation by performing isotope enrichment analysis on the SP:SAMPLEPREP_SUMMARY CRISPR/Cas9 knockout pancreatic cancer cell lines. Each cell line expressing SP:SAMPLEPREP_SUMMARY PKM1 or PKM2 will be seeded on 6-well plates so that they are ~70% confluent at SP:SAMPLEPREP_SUMMARY the time of labeled media addition. Unlabeled media will be aspirated, and cell SP:SAMPLEPREP_SUMMARY will be washed with PBS. Media labeled with 13C-glucose, 13C-glutamine, or SP:SAMPLEPREP_SUMMARY 13C-serine labeled media will be added, and cells will be incubated for 1 hour SP:SAMPLEPREP_SUMMARY or 24 hours. Polar metabolites and fatty acids will be extracted at the end of SP:SAMPLEPREP_SUMMARY the incubation period using methanol, water, and chloroform. The methanol/water SP:SAMPLEPREP_SUMMARY fraction containing polar metabolites will be separated from the chloroform SP:SAMPLEPREP_SUMMARY fraction containing fatty acids, and each fraction will be dried down under SP:SAMPLEPREP_SUMMARY nitrogen. Dried down samples will be sent to the Mayo Clinic Metabolomics SP:SAMPLEPREP_SUMMARY Resource Core for analysis of TCA cycle intermediates, amino metabolites, free SP:SAMPLEPREP_SUMMARY fatty acids, and sphingolipids. TCA cycle intermediates and amino metabolites SP:SAMPLEPREP_SUMMARY (polar metabolites) should become labeled faster than fatty acids and SP:SAMPLEPREP_SUMMARY sphingolipids; therefore, polar metabolites are proposed to be analyzed at 1 SP:SAMPLEPREP_SUMMARY hour and 24 hours after 13C-label addition. Fatty acids are proposed to be SP:SAMPLEPREP_SUMMARY analyzed for the 24 hr time point only, due to slow labeling. Statistical SP:SAMPLEPREP_SUMMARY analysis will be performed by the Mayo Clinic Metabolomics Resource Core. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (150 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantum Ultra MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS % enrichment: MPE MS_METABOLITE_DATA_START Samples ms5770-1 ms5770-2 ms5770-3 ms5770-4 ms5770-5 ms5770-6 ms5770-7 ms5770-8 ms5770-9 ms5770-10 ms5770-11 ms5770-12 ms5770-13 ms5770-14 ms5770-15 ms5770-16 ms5770-17 ms5770-18 Factors experiment:20_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- experiment:21_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- experiment:22_PSPH B7 + Dox (PKM1)_0 hr_No label | tracer:No Label | time:- experiment:23_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- experiment:24_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- experiment:25_PSPH B7 - Dox (PKM2)_0 hr_No label | tracer:No Label | time:- experiment:26_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:27_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:28_PSPH B7 + Dox (PKM1)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:29_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:30_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:31_PSPH B7 - Dox (PKM2)_24 hr_13C-glucose | tracer:13C-glucose | time:24 experiment:32_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 experiment:33_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 experiment:34_PSPH B7 + Dox (PKM1)_48 hr_13C-glucose | tracer:13C-glucose | time:48 experiment:35_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 experiment:36_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 experiment:37_PSPH B7 - Dox (PKM2)_48 hr_13C-glucose | tracer:13C-glucose | time:48 13C-myristate m0 100 33.45803813 26.07187671 25.40791022 28.20520421 27.31459284 25.94173835 18.85337095 17.37100157 15.66385075 21.20342678 19.06960269 18.07480146 13C-myristate m1 9.35589E-13 1.099513392 2.064435568 2.267814153 1.928998859 1.992992033 1.85934806 1.88598981 2.411655796 2.253451711 2.01762117 2.25011817 2.143591397 13C-myristate m2 9.69814E-13 1.874942198 2.110387362 2.239107715 2.295597298 2.322927319 2.308364821 2.542887338 2.481047829 2.554479355 2.406984351 2.625339473 2.549984194 13C-myristate m3 9.64248E-13 1.748392215 1.957893666 1.952863335 1.859183434 1.852707675 1.850916925 2.140693689 2.26985418 2.265188883 2.121034365 2.104381261 2.134364602 13C-myristate m4 9.65035E-13 1.769045175 2.127775001 2.290507687 2.306991176 2.345821725 2.309740782 2.501319665 2.504189852 2.552563848 2.390107317 2.649123737 2.551490008 13C-myristate m5 9.65648E-13 1.765674611 1.955056003 1.944474861 1.857323953 1.848971308 1.85069237 2.271339885 2.373359956 2.383635608 2.305077552 2.267521007 2.300228644 13C-myristate m6 9.61005E-13 8.010527517 8.847894933 8.641523426 8.322266088 8.687359151 9.307807243 9.64282771 9.32839761 9.63036746 8.821056703 9.624166065 9.386152509 13C-myristate m7 9.61737E-13 1.284381234 1.307577263 1.406724983 1.475569948 1.257997666 1.291974692 1.498619815 1.607950147 1.645252903 1.520938543 1.549227956 1.571366281 13C-myristate m8 9.61779E-13 4.096099819 4.173503374 4.427687159 4.307061792 4.652671619 4.786598241 4.884521513 4.774029551 4.860638443 4.727053749 5.078023471 5.085340489 13C-myristate m9 9.60783E-13 3.027054323 3.125806777 3.209651923 3.187886427 2.939836236 3.125427248 3.533356132 3.487242396 3.720551585 3.41137787 3.374316601 3.609899263 13C-myristate m10 9.67005E-13 7.804670347 8.656828406 8.43504653 8.105118293 8.509329995 9.09977051 9.436866659 9.146611996 9.412182773 8.640508493 9.443537023 9.172414264 13C-myristate m11 9.28859E-13 4.261755778 4.628467506 4.453618008 4.606796881 4.572896654 4.373187659 4.843920838 5.235257206 5.276811166 4.85472044 4.862908347 5.009170416 13C-myristate m12 9.39685E-13 13.70207097 15.15739753 15.17277239 14.22874862 14.12723553 14.73691793 15.79583606 15.75828159 16.54612699 15.52381317 15.11745373 16.21717813 13C-myristate m13 9.0973E-13 3.509704488 3.707815078 3.945115536 3.625600376 3.89935747 3.507747623 4.508566956 4.861652267 4.800585323 4.652761042 4.464447976 4.565819669 13C-myristate m14 1.08734E-12 12.5881298 14.10728483 14.20518208 13.68765265 13.67530278 13.64976755 15.65988298 16.38946805 16.4343132 15.40351845 15.51983249 15.62819867 13C-myristate m15 0 0 0 0 0 0 0 0 0 0 0 0 0 13C-myristate m16 0 0 0 0 0 0 0 0 0 0 0 0 0 13C-palmitate m0 99.99846593 70.10193484 52.12648819 58.54773402 66.75432577 62.21055229 49.67521351 46.9987925 38.93527153 45.16886258 52.24942161 42.94737463 50.78690318 13C-palmitate m1 0.001232001 0 0.106446029 0 0 0 0 0 0.024359303 0.036056011 0.072909396 0.096018747 0 13C-palmitate m2 0 0.342056896 0.387892427 0.352363123 0.409104395 0.396697904 0.567595953 0.3292996 0.358631299 0.358181498 0.241471604 0.362323146 0.382477831 13C-palmitate m3 0 0.016649563 0.023324634 0.044874256 0 0.045790344 0.014152109 0.006387848 0.041997335 0.02035138 0.00150564 0.038708402 0.01763511 13C-palmitate m4 0.000301363 0.082320101 0.098880058 0.127415471 0.102906128 0.124158078 0.211890974 0.16034468 0.100275411 0.115474538 0.119603549 0.237533569 0.151789294 13C-palmitate m5 0 0.028023406 0.049993433 0.041632918 0.051061697 0.053678444 0.081655361 0.053630527 0.048515136 0.045531072 0.050430991 0.05303597 0.067955028 13C-palmitate m6 6.71075E-07 0.182054067 0.22459455 0.272447744 0.179122857 0.289240208 0.46445551 0.333411978 0.275043943 0.309589544 0.259420983 0.512030219 0.405872124 13C-palmitate m7 0 0.099424541 0.142047423 0.133118834 0.123652014 0.130113946 0.204500033 0.158269266 0.169241155 0.168694685 0.160471035 0.218436071 0.184335091 13C-palmitate m8 3.78552E-08 0.558853038 0.851874226 0.856263604 0.698562406 0.869593753 1.266707907 1.020793745 1.071229442 1.092078904 0.942120783 1.340047178 1.151888555 13C-palmitate m9 0 0.486284923 0.580267399 0.524282683 0.396339236 0.447580295 0.594464154 0.747178928 0.705268547 0.721432307 0.584469972 0.654870526 0.591529037 13C-palmitate m10 5.19112E-10 0.486800463 0.615912613 0.570563263 0.446580039 0.509694851 0.700511773 0.78050337 0.754290491 0.770947968 0.634209874 0.757222015 0.674284523 13C-palmitate m11 0 1.395091894 2.228786035 1.960365408 1.536846174 1.767880163 2.35036405 2.446050962 2.677053376 2.568979849 2.077409032 2.694192409 2.186219405 13C-palmitate m12 5.96629E-12 5.388210933 8.484321089 7.505695255 5.732636521 6.710681403 8.949049954 9.199097259 9.959108798 9.199549158 7.902928946 9.600105287 8.290703242 13C-palmitate m13 0 2.104297874 3.510992119 2.964564198 2.245654244 2.544134615 3.389664468 3.718264354 4.508078286 3.989197338 3.397764628 3.895290725 3.358449431 13C-palmitate m14 1.12532E-12 9.381761097 15.40250958 12.91469194 10.59905668 11.94772199 15.78172066 16.48555658 19.41656623 17.01808734 14.7221592 17.36145501 15.18743021 13C-palmitate m15 9.77535E-13 1.343172136 2.217877946 1.839944868 1.588191059 1.758410879 2.176339782 2.680174615 3.325979462 2.796322311 2.602906803 3.025963922 2.610165359 13C-palmitate m16 9.95117E-13 7.821893459 12.69631315 11.12447433 8.951026255 10.0068045 13.23577833 14.6140609 17.31139416 15.34297123 13.70299718 15.88747959 13.67497353 13C-palmitate m17 9.56876E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-palmitate m18 1.20059E-12 0.18117077 0.251479094 0.219568082 0.184934522 0.187266337 0.335935475 0.268182889 0.317696096 0.277692294 0.277798773 0.317912577 0.27738905 13C-palmitate m19 0 0 0 0 0 0 0 0 0 0 0 0 0 13C-oleate m0 100 36.32607801 35.99156849 36.426777 39.84143801 41.09746049 40.66736661 23.29587036 22.33476525 21.69415065 24.8630594 24.84247599 24.33543375 13C-oleate m1 6.27656E-13 0.311111857 0.230039232 0.231269576 0.262029479 0.23336375 0.303345228 0.125782467 0.252330018 0.210133374 0.213765214 0.495509008 0.079653681 13C-oleate m2 6.54834E-13 8.109222423 8.344731497 8.367419282 7.910244066 8.208227981 8.306646296 6.269049618 6.656978603 6.8911169 6.750999393 6.743072335 6.866979339 13C-oleate m3 6.55202E-13 0.004614634 0 0 0 0 0 0 0 0 0 0 0 13C-oleate m4 6.51968E-13 0.32105925 0.328360493 0.294181495 0.31820442 0.360814812 0.310299749 0.259735622 0.168604164 0.220990106 0.255971167 0.210683366 0.182687663 13C-oleate m5 6.7135E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-oleate m6 6.14969E-13 0.006649975 0 0 0 0 0.031093093 0 0 0 0.010306971 0.048141944 0.071924562 13C-oleate m7 6.61213E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-oleate m8 6.56937E-13 0.142537734 0.158420966 0.202202587 0.22448105 0.261516678 0.31743837 0.35720077 0.252403927 0.260392261 0.215234555 0.479876296 0.473054565 13C-oleate m9 6.61308E-13 0.166148248 0.134251555 0.097693454 0.08480989 0.101127595 0.150624705 0.164082501 0.052176125 0.15321977 0.109555643 0.116156493 0.137917001 13C-oleate m10 6.60573E-13 1.189439014 1.132804384 1.272814187 1.111339704 1.058483508 1.400763909 1.459321515 1.192064091 1.492612241 1.326863933 1.576241348 1.665336247 13C-oleate m11 6.60655E-13 0.785334519 0.66047281 0.526088877 0.558722354 0.583263482 0.60549638 0.964580933 0.798951828 0.841583472 0.874605755 0.723580411 0.82988181 13C-oleate m12 6.58649E-13 3.560834547 3.469293634 3.659837073 2.958374601 3.103252835 3.596876911 4.644345959 4.187792347 4.785667813 4.284755249 4.642674278 4.373071857 13C-oleate m13 6.75819E-13 1.640355499 1.639117255 1.781422967 1.462386155 1.405043364 1.596703207 2.379011171 2.44627962 2.26980228 2.169259093 2.035775175 2.110866049 13C-oleate m14 6.11496E-13 8.326085052 8.029134892 7.709196524 7.251692565 7.273121762 7.796794386 10.01226586 9.454455845 10.37307951 9.200736762 9.597519695 9.752817627 13C-oleate m15 6.21071E-13 2.690335882 2.767085666 2.762133935 2.307578937 1.987703334 2.199159984 3.443413166 3.598692474 3.572888977 3.537836289 3.378777402 3.155778775 13C-oleate m16 6.23472E-13 11.98245783 11.63207771 11.5460723 10.57482821 9.979144758 10.02596648 14.1749351 14.26429236 14.44113798 13.72570161 13.47799232 14.0264016 13C-oleate m17 6.20842E-13 1.212588004 1.152856249 1.127146441 0.949855631 0.95552564 0.960196765 1.7084913 1.818883334 1.486772296 1.685304332 1.582925672 1.617860592 13C-oleate m18 6.10707E-13 7.883482488 7.925029062 7.658851436 7.462525083 7.211611448 7.133218189 10.07976662 10.54221649 10.24890106 9.904679281 9.691607188 9.953182303 13C-oleate m19 7.45666E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-stearate m0 100 74.16060136 55.23782595 61.47036439 76.46024869 72.29184392 52.44616184 44.97279584 38.8411683 38.8411683 48.48203552 40.14888116 48.31090993 13C-stearate m1 6.27656E-13 0 0 0.32749105 0.023298418 0.021196208 0.129458096 0 0.047900374 0.047900374 0 0.098592224 0 13C-stearate m2 6.54834E-13 2.184160758 3.699245049 3.028352576 2.096412434 2.689151778 4.209108081 2.858885653 3.066214283 3.066214283 2.936498418 3.314832999 2.835720512 13C-stearate m3 6.55202E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-stearate m4 6.51968E-13 0.095150815 0.139689124 0.121413473 0.107641725 0.130536354 0.227523898 0.021098762 0.03476116 0.03476116 0.048344561 0.118437348 0.103070714 13C-stearate m5 6.7135E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-stearate m6 6.14969E-13 0 0 0.019034562 0.019793493 0.044636531 0.07697974 0.004118236 0 0 0 0.107605235 0.062924214 13C-stearate m7 6.61213E-13 0 0 0 0 0 0 0 0 0 0 0 0 13C-stearate m8 6.56937E-13 0.1151138 0.097533175 0.143086336 0.094084981 0.15952386 0.302296764 0.233086031 0.196431935 0.196431935 0.178141116 0.3934624 0.315228609 13C-stearate m9 6.61308E-13 0.042702612 0.03120988 0.034188247 0.053993655 0.027356534 0.047152005 0.046350681 0.046207561 0.046207561 0.011751144 0.045029843 0.048384782 13C-stearate m10 6.60573E-13 0.432359865 0.621817119 0.752892366 0.434075827 0.519349415 0.977641195 0.920224841 0.970794268 0.970794268 0.753765369 1.196013782 1.037530583 13C-stearate m11 6.60655E-13 0.305527806 0.418914144 0.312635639 0.222657335 0.259210055 0.448313245 0.545226726 0.574233793 0.574233793 0.504841321 0.59049971 0.50996701 13C-stearate m12 6.58649E-13 1.376139236 2.35041001 1.984405864 1.204804327 1.500977354 2.724532851 2.978725439 3.219444133 3.219444133 2.551418619 3.39071422 2.884669319 13C-stearate m13 6.75819E-13 0.82000991 1.339526878 1.050009148 0.649351403 0.712975346 1.295653919 1.597798147 1.772667512 1.772667512 1.372334895 1.51137748 1.368654227 13C-stearate m14 6.11496E-13 3.422298775 5.56495626 4.892093264 2.80340647 3.438656343 6.032462455 6.839638155 7.517302386 7.517302386 6.048388765 7.215368171 6.31589498 13C-stearate m15 6.21071E-13 1.2311907 2.144145146 1.768943629 1.053269996 1.219952628 2.162697501 2.599479305 2.826492568 2.826492568 2.451746235 2.619093256 2.236578932 13C-stearate m16 6.23472E-13 4.937408275 8.774690106 7.396971506 4.273353993 5.352175235 8.976136313 10.67514912 11.93850529 11.93850529 10.00166942 11.19758345 9.700770747 13C-stearate m17 6.20842E-13 0.46940464 1.033739517 0.882603796 0.608760628 0.563527264 0.889314987 1.33329949 1.529387907 1.529387907 1.352390233 1.482853931 1.220095867 13C-stearate m18 6.10707E-13 3.501464038 6.24157859 5.242711753 3.279387681 3.81008641 6.394604933 8.107398294 9.121156433 9.121156433 7.843405815 8.750839124 7.736080512 13C-stearate m19 7.45666E-13 0 0 0 0 0 0 0 0 0 0 0 0 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name 13C-myristate m0 13C-myristate m1 13C-myristate m2 13C-myristate m3 13C-myristate m4 13C-myristate m5 13C-myristate m6 13C-myristate m7 13C-myristate m8 13C-myristate m9 13C-myristate m10 13C-myristate m11 13C-myristate m12 13C-myristate m13 13C-myristate m14 13C-myristate m15 13C-myristate m16 13C-palmitate m0 13C-palmitate m1 13C-palmitate m2 13C-palmitate m3 13C-palmitate m4 13C-palmitate m5 13C-palmitate m6 13C-palmitate m7 13C-palmitate m8 13C-palmitate m9 13C-palmitate m10 13C-palmitate m11 13C-palmitate m12 13C-palmitate m13 13C-palmitate m14 13C-palmitate m15 13C-palmitate m16 13C-palmitate m17 13C-palmitate m18 13C-palmitate m19 13C-oleate m0 13C-oleate m1 13C-oleate m2 13C-oleate m3 13C-oleate m4 13C-oleate m5 13C-oleate m6 13C-oleate m7 13C-oleate m8 13C-oleate m9 13C-oleate m10 13C-oleate m11 13C-oleate m12 13C-oleate m13 13C-oleate m14 13C-oleate m15 13C-oleate m16 13C-oleate m17 13C-oleate m18 13C-oleate m19 13C-stearate m0 13C-stearate m1 13C-stearate m2 13C-stearate m3 13C-stearate m4 13C-stearate m5 13C-stearate m6 13C-stearate m7 13C-stearate m8 13C-stearate m9 13C-stearate m10 13C-stearate m11 13C-stearate m12 13C-stearate m13 13C-stearate m14 13C-stearate m15 13C-stearate m16 13C-stearate m17 13C-stearate m18 13C-stearate m19 METABOLITES_END #END