#METABOLOMICS WORKBENCH araskind_20170619_114634 DATATRACK_ID:1004 STUDY_ID:ST000707 ANALYSIS_ID:AN001098 PROJECT_ID:PR000504 VERSION 1 CREATED_ON July 2, 2017, 11:13 am #PROJECT PR:PROJECT_TITLE Amino Acid Analysis of Hamsters treated with antibiotics PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Amino Acid Analysis of Hamsters treated with antibiotics PR:INSTITUTE Kansas State University PR:DEPARTMENT Biology PR:LABORATORY Govind Lab PR:LAST_NAME Govind PR:FIRST_NAME Revathi PR:ADDRESS Manhattan, KS PR:EMAIL rgovind@ksu.edu PR:PHONE 785-532-2816 #STUDY ST:STUDY_TITLE Amino Acid Analysis of hamster's gut contents ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Hamsters were treated with Clindamycin, or a Saline, or nothing at all. After ST:STUDY_SUMMARY four days animals were sacrificed and their cecal contents were collected to ST:STUDY_SUMMARY record change in their Microbiome. Along with the Microbiome analysis we are ST:STUDY_SUMMARY also performing metabolomics analysis to understand the influence of change in ST:STUDY_SUMMARY the microbiome with the metabolic changes (if any). The cecal contents collected ST:STUDY_SUMMARY were frozen immediately (using liquid nitrogen) and was frozen in -80C until ST:STUDY_SUMMARY analysis. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 20 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mesocricetus auratus SU:TAXONOMY_ID 10026 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0013396 S00020057 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013397 S00020058 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013398 S00020059 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013399 S00020060 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013400 S00020061 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013401 S00020062 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013402 S00020063 Treatment:Antibiotic treated SUBJECT_SAMPLE_FACTORS SU0013403 S00020064 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013404 S00020065 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013405 S00020066 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013406 S00020067 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013407 S00020068 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013408 S00020069 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013409 S00020070 Treatment:Saline treated SUBJECT_SAMPLE_FACTORS SU0013410 S00020071 Treatment:No treatment SUBJECT_SAMPLE_FACTORS SU0013411 S00020072 Treatment:No treatment SUBJECT_SAMPLE_FACTORS SU0013412 S00020073 Treatment:No treatment SUBJECT_SAMPLE_FACTORS SU0013413 S00020074 Treatment:No treatment SUBJECT_SAMPLE_FACTORS SU0013414 S00020075 Treatment:No treatment SUBJECT_SAMPLE_FACTORS SU0013415 S00020076 Treatment:No treatment #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A005 - Amino Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A005 - Amino Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00020057 S00020058 S00020059 S00020060 S00020061 S00020062 S00020063 S00020064 S00020065 S00020066 S00020067 S00020068 S00020069 S00020070 S00020071 S00020072 S00020073 S00020074 S00020075 S00020076 Factors Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Antibiotic treated Treatment:Saline treated Treatment:Saline treated Treatment:Saline treated Treatment:Saline treated Treatment:Saline treated Treatment:Saline treated Treatment:Saline treated Treatment:No treatment Treatment:No treatment Treatment:No treatment Treatment:No treatment Treatment:No treatment Treatment:No treatment L-PROLINE .966636756 2.063429242 2.275722529 1.96907276 .157184841 .208417527 .132963009 .263800329 3.747901604 2.185385077 2.442013388 3.322273256 2.282444014 2.452119338 1.192830403 1.672407113 1.816078901 1.219847449 1.2926223 2.715166213 L-GLUTAMIC ACID .539794678 .551441689 .375598611 .385880359 1.166185278 2.354133641 1.256132024 1.307730647 .971857216 .472418236 .428727877 .526668048 .472475427 .729811349 .251634512 .254963986 .249955932 .633566378 .492635306 .886077219 L-ALANINE 3.951341628 6.820190765 9.185667564 7.405257353 .552560619 .650405434 .454654071 .798045136 13.71423111 5.178941128 9.455346442 11.13872456 8.529095096 9.524049678 2.642839978 5.700048571 6.047493949 2.311129649 4.690549022 9.510866516 L-SERINE .770823354 .747151643 1.788776029 1.483716723 .147043024 .126936661 .060542515 .430847314 2.156829838 1.0059376 1.829945002 2.204696022 1.410623915 1.522946125 .750502069 .841434306 1.78669677 .425267383 1.67660906 2.371009002 L-ASPARTIC ACID .026517065 .070411282 .094372867 .096876719 .180227158 .283318418 .372329397 .698078307 .222102474 .068289752 .101230387 .249606431 .078073017 .112752847 .101866246 .072314396 .107032126 .049426918 .116249349 .168912904 L-LYSINE .700510283 1.683098779 1.801551904 1.517717773 .289279963 .799522586 .130352934 .410473365 2.911900052 1.436132343 .096603503 3.170748615 2.933820745 2.047322742 .980874306 1.899403644 .603624108 .774778724 2.724477393 4.646342911 L-TYROSINE 0 0 .04230421 0 .052150534 .20638964 .066125683 .098125203 .296035036 .020703474 .012037913 .082901867 .028806834 .034256524 .077698157 .018941275 .082794091 .053327368 .01769049 .049829005 L-LEUCINE .343512171 .643267391 .397224871 .801377981 .233316611 .696890673 .209562448 .425464979 .535851758 .166201078 .102010556 .953130456 .400839573 .852283467 .644552024 .728902509 .832606423 .1477779 1.122034095 1.756963713 2-AMINOISOBUTYRIC ACID .038059946 .056000687 .028613899 .033147882 .028096403 .047947807 .009505174 .025912828 .094372742 .064715908 .023382228 .041350666 .07970246 .026521219 .011686286 .010832578 .010606106 .053637267 .027086015 .066036048 L-METHIONINE .122777267 .185734758 .213171247 .182075419 .045693969 .116619559 0 .061144877 .287582178 .118777526 .038662905 .288119421 .135824179 .26625977 .116007867 .178912184 .262947251 .067842377 .329782012 .405200725 L-PHENYLALANINE .052007235 .057057251 .022323435 .066247676 .080248631 .236059722 .093148319 .205166045 .072503777 .024309984 .014785689 .108757477 .044976243 .0574923 .17378634 .054844814 .137130239 .059235128 .101952364 .161479162 L-ORNITHINE .222221428 .614267063 .715441711 .797495321 .015736374 .011484498 .027105458 .016235408 1.023475607 .586403661 .036185827 .916026885 1.030771107 .928875344 .364976366 .793239273 .29125192 .165921684 .84781516 1.679244955 L-HISTIDINE 0 0 0 .032358531 .040933682 .040993168 .089475443 .116053139 .072504451 .048971581 .027877163 .136701231 .034163373 .030670023 .021750417 .017231552 .038271738 .028666831 .020070528 .057901621 L-VALINE 1.339740217 2.346329829 3.080748568 2.473961461 .319147089 .842514612 .123192522 .311617507 4.406319987 2.03301639 2.803551427 3.740011596 3.116998603 2.779677895 1.306842732 2.376330319 2.188563194 .976094094 1.831426399 3.084631774 L-THREONINE .535661476 1.396476222 1.61594872 1.566153447 .097459331 .221282991 .102754021 .337935749 2.456825925 1.366193338 2.139706287 2.711746148 2.177028222 2.030434518 .753653017 1.367231027 1.657113489 .505455825 1.593273387 2.511315793 L-TRYPTOPHAN .060832868 .027886859 .032371574 .077048156 .010852136 .011552115 .010458645 .017570204 .036695972 .005411729 .004729662 .029412178 .018686572 .04204505 .033581383 .031176926 .024065629 .014319176 .054787196 .058608779 L-ISOLEUCINE .896845548 1.382706742 1.607749471 1.709101943 .148434254 .324368297 .195047193 .327483914 2.306223298 .617391083 1.196750111 2.232118215 1.69659246 2.001678571 1.011522906 1.638105144 1.447458999 .183772443 1.463311711 2.622081818 GLYCINE 1.597158013 2.449684696 3.227501386 2.841241889 .189167234 1.496832204 .147083644 .327285033 5.557417595 2.944413528 3.383620489 5.378751534 3.932543976 3.694112883 1.871867526 2.326984782 2.817261259 1.461958125 1.960586229 3.534585362 4-HYDROXYPROLINE .034562544 .128114145 .038753419 .13220864 .006092071 .015251567 .024476339 .002120373 .238295777 .129526885 .062510736 .145941378 .059443204 .029820741 .044989267 .055880548 .026079768 .08107436 .05210551 .079987106 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type L-PROLINE 145742 L-GLUTAMIC ACID 33032 L-ALANINE 5950 L-SERINE 5951 L-ASPARTIC ACID 5960 L-LYSINE 5962 L-TYROSINE 6057 L-LEUCINE 6106 2-AMINOISOBUTYRIC ACID 6119 L-METHIONINE 6137 L-PHENYLALANINE 6140 L-ORNITHINE 6262 L-HISTIDINE 6274 L-VALINE 6287 L-THREONINE 6288 L-TRYPTOPHAN 6305 L-ISOLEUCINE 6306 GLYCINE 750 4-HYDROXYPROLINE 825 METABOLITES_END #END