#METABOLOMICS WORKBENCH araskind_20170619_175040 DATATRACK_ID:1034 STUDY_ID:ST000729 ANALYSIS_ID:AN001142 PROJECT_ID:PR000526 VERSION 1 CREATED_ON July 3, 2017, 10:43 am #PROJECT PR:PROJECT_TITLE Ethicon Rat Study PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Amino acid analysis in plasma between Sham and VSG PR:INSTITUTE University of Michigan PR:DEPARTMENT Surgery PR:LABORATORY Seeley Lab PR:LAST_NAME Seeley PR:FIRST_NAME Randy PR:ADDRESS Ann Arbor, MI PR:EMAIL seeleyrj@umich.edu PR:PHONE 734-615-2880 #STUDY ST:STUDY_TITLE Metabolomics of rats after bariatric or sham surgery ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY We would like to examine the levels of amino acids in rats who have underwent ST:STUDY_SUMMARY bariatric (VSG, vertical sleeve gastrectomy) or sham (control) surgery. Plasma ST:STUDY_SUMMARY was collected after an overnight fast and once again after refeeding the rats ST:STUDY_SUMMARY for 2 hours. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 24 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0017524 S00027572 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017525 S00027573 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017526 S00027574 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017527 S00027575 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017528 S00027576 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017529 S00027577 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017530 S00027578 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017531 S00027579 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017532 S00027580 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017533 S00027581 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017534 S00027582 Diet:fasting overnight | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017535 S00027583 Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery SUBJECT_SAMPLE_FACTORS SU0017536 S00027584 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017537 S00027585 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017538 S00027586 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017539 S00027587 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017540 S00027588 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017541 S00027589 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017542 S00027590 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017543 S00027591 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017544 S00027592 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017545 S00027593 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017546 S00027594 Diet:fasting overnight | Surgery:SHAM surgery SUBJECT_SAMPLE_FACTORS SU0017547 S00027595 Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A005 - Amino Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A005 - Amino Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00027572 S00027573 S00027574 S00027575 S00027576 S00027577 S00027578 S00027579 S00027580 S00027581 S00027582 S00027583 S00027584 S00027585 S00027586 S00027587 S00027588 S00027589 S00027590 S00027591 S00027592 S00027593 S00027594 S00027595 Factors Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:VSG surgery Diet:refed for 2 hours (45% HFD food) | Surgery:VSG surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery Diet:fasting overnight | Surgery:SHAM surgery Diet:refed for 2 hours (45% HFD food) | Surgery:SHAM surgery L-PROLINE 173.4414498 381.7842574 181.6496847 156.4874617 368.7266337 329.131305 175.0582359 169.492558 197.8798334 181.0409331 143.9397925 255.6548925 144.3655518 163.3802295 178.5707159 314.1544643 158.8795433 308.0202584 166.9789067 280.7665901 182.3173192 297.3711097 173.260446 297.2598315 L-GLUTAMIC ACID 266.458605 294.9024369 180.2054074 115.2467742 185.3627613 94.61017429 102.3020073 86.39986969 127.2505518 141.0421862 84.28622483 142.7342389 97.5304568 65.89802077 74.46000631 67.32550833 123.4945404 148.1011392 85.85281925 56.37854567 69.07795332 48.3422524 50.8060915 39.80617287 L-ALANINE 626.3512464 869.6739733 467.2804337 297.3353704 471.5930206 475.9383887 463.6087678 260.3583559 291.6375927 187.8590254 329.3843455 456.6490228 435.610063 308.6703224 475.8773718 662.8577819 400.6195536 510.6097224 480.8365459 456.5969725 401.1431516 471.8312127 443.3412854 363.8185529 L-SERINE 875.3393492 883.493214 964.075683 913.736704 1080.133285 695.948119 769.4972654 894.2666816 565.818072 719.8591884 1151.047912 761.231583 427.4615468 188.6809164 319.0068937 276.8675725 534.6244162 437.666802 336.6145223 467.5957444 671.5865429 437.243828 393.1338642 446.5123886 L-ASPARTIC ACID 52.85417514 41.08745176 21.57404364 22.52878978 39.00488123 34.05307794 24.15370678 12.99610292 64.01366787 52.83210753 31.41565754 16.87347808 26.46513296 19.83570312 6.733165496 10.0297241 22.60105356 35.07851644 9.505636737 6.125731308 19.51068749 7.559676716 14.03832701 13.94357053 L-LYSINE 445.4202771 473.197194 383.5136557 432.6168848 601.780028 405.4278795 415.4954357 441.6706943 544.2214187 508.52881 529.0847673 504.843136 388.2227459 265.9988716 372.1463487 439.6291032 335.2403185 303.6979491 425.7140694 463.077436 635.0351569 448.2000115 509.4860419 457.8270725 L-TYROSINE 53.00747691 83.18664921 67.78116174 57.48043042 106.2791665 70.69087593 62.20534278 66.19179673 81.35993705 69.27073585 58.8310794 75.87838056 61.35375903 46.68717766 53.06563829 60.15054299 32.71734468 60.50837237 53.05977223 47.67512466 65.00664218 77.99223277 48.4483799 110.41312 L-LEUCINE 79.03638971 102.0485823 68.19956601 77.1529248 88.64978951 46.63317971 60.72925852 61.07299082 91.08958475 82.65293688 81.49636956 83.93629627 94.23831746 53.95017281 92.94861027 107.6313739 96.92214713 106.2422281 79.23673073 86.17385934 110.0241101 89.50938264 101.1104734 106.2952082 2-AMINOISOBUTYRIC ACID 14.20032124 11.13783355 14.60134717 16.88814388 8.675068725 8.094612156 19.47054753 16.8773016 52.46987701 39.09341241 20.98241927 16.16437817 8.448116338 4.446237929 18.7667953 13.76590293 11.98338281 7.826813666 10.56261465 10.22041932 23.01577817 13.04289832 19.80053333 8.264153192 L-METHIONINE 29.32110184 33.4224896 35.4465108 20.94594763 38.92100833 25.51793651 35.22759339 33.20263835 23.24051556 27.70617693 30.6234626 38.31725905 45.37796381 33.35545738 43.32243826 48.80555746 39.65543172 57.51010021 36.99742265 46.03353959 50.11905178 58.66245543 36.03853666 80.14144757 L-PHENYLALANINE 54.19330107 60.65592564 55.580498 51.64224166 66.62939472 46.05556211 42.37683608 53.01367387 63.86626653 66.83707098 53.56743963 64.38354523 65.33535847 49.31555865 54.47051691 67.70516251 57.472026 80.55247475 52.27670436 66.85190511 66.34084613 64.67660883 55.67946065 93.63739477 L-ORNITHINE 42.44180826 40.48237223 41.71918084 42.16380504 42.9533725 29.27971311 25.20308401 34.37557774 50.91939292 56.83689055 37.77727961 31.50261759 21.33066267 16.57244773 19.06969683 20.90622284 33.23429213 28.0258378 22.81417319 23.8372436 24.2706139 25.79513987 17.10924216 24.88137962 L-ASPARAGINE 104.2190182 164.9125076 82.72073672 84.83940711 114.32213 78.79803912 68.86448248 101.9303522 24.73450662 24.19787118 121.3943621 133.6036454 95.61837339 72.92318392 81.74109017 106.8612471 103.2084182 136.3024837 109.1922111 130.4471157 183.3367281 137.9418938 107.6810834 202.1517431 L-HISTIDINE 24.76209878 37.60702717 28.88575905 32.56381117 49.50001163 31.23511826 40.85802328 47.06762962 55.03615236 50.16194943 56.58752498 55.5447138 37.58779336 26.93827936 41.64994598 42.61928096 29.27655852 20.04221989 17.68621787 26.04417039 33.4724243 23.28182482 35.0926789 56.33248679 L-VALINE 119.2392623 172.6285909 97.81029306 105.2005066 132.8863914 98.84267658 104.7067935 81.25917736 125.9681247 90.28733218 101.6795539 116.7084605 142.3899544 91.25749638 168.0729388 185.1228855 147.6304 137.8909833 147.0365239 134.1033051 132.4919313 122.5672251 171.8615218 127.8088558 L-THREONINE 285.8805327 283.3512508 253.9103464 252.5494831 530.8733448 407.2795562 249.8792874 308.6115681 307.2439102 338.4314911 289.2555597 279.5826774 342.3530156 200.92869 329.0830228 310.4306674 387.5041185 418.5759296 339.2740674 406.7486749 550.983805 442.7499494 387.4619461 542.0837561 L-TRYPTOPHAN 56.54822652 74.95302921 63.13491092 42.95315721 72.62684604 30.69065332 24.65350563 42.41731631 30.04744073 49.54142517 47.54346484 53.72283471 48.771141 44.29708457 61.5975684 53.56490102 68.00537484 63.36279936 47.79849856 75.50778379 66.43341171 76.59814949 57.66496109 107.264309 L-ISOLEUCINE 65.34032986 89.35967819 59.86332993 30.09662426 61.13634851 40.14206036 48.59341803 44.25432716 82.48803105 58.00284877 63.71154132 67.25979082 74.48020829 34.76754305 84.83497944 63.56678223 84.58285423 74.20566708 77.39972216 67.61812937 87.58147673 67.55610759 93.31914019 64.74138787 GLYCINE 711.2221272 482.3767177 714.8254075 639.6445605 464.8732346 583.2563777 604.9179328 448.4687032 666.9158074 545.3023816 691.2705442 382.2811857 599.4973447 219.5741142 391.9982998 287.936319 514.7462663 392.4710132 599.1472051 361.2017401 657.4019782 302.058558 638.4918934 341.5012834 4-HYDROXYPROLINE 32.48971893 33.91231151 43.63520577 41.16526614 52.23229231 43.75566262 28.14822034 33.16205986 61.47631031 80.71523677 43.50342547 30.40858426 23.54727592 11.46670774 22.7888179 16.98508403 18.03315778 12.41550135 24.43006526 29.86793178 37.13369478 24.10201244 37.5280997 40.59686154 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type L-PROLINE 145742 L-GLUTAMIC ACID 33032 L-ALANINE 5950 L-SERINE 5951 L-ASPARTIC ACID 5960 L-LYSINE 5962 L-TYROSINE 6057 L-LEUCINE 6106 2-AMINOISOBUTYRIC ACID 6119 L-METHIONINE 6137 L-PHENYLALANINE 6140 L-ORNITHINE 6262 L-ASPARAGINE 6267 L-HISTIDINE 6274 L-VALINE 6287 L-THREONINE 6288 L-TRYPTOPHAN 6305 L-ISOLEUCINE 6306 GLYCINE 750 4-HYDROXYPROLINE 825 METABOLITES_END #END