#METABOLOMICS WORKBENCH araskind_20170619_180759 DATATRACK_ID:1043 STUDY_ID:ST000767 ANALYSIS_ID:AN001213 PROJECT_ID:PR000553 VERSION 1 CREATED_ON July 5, 2017, 12:57 pm #PROJECT PR:PROJECT_TITLE Metabolic uniqueness of neural stem/progenitor cells Studies PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Cell-intrinsic metabolic uniqueness of regional neural stem/progenitor cells PR:INSTITUTE Cincinnati Children's Hospital Medical Center PR:DEPARTMENT Oncology PR:LABORATORY Dasgupta Lab PR:LAST_NAME Dasgupta PR:FIRST_NAME Biplab PR:ADDRESS Cincinnati, OH PR:EMAIL biplab.dasgupta@cchmc.org PR:PHONE 513-803-1370 #STUDY ST:STUDY_TITLE Metabolite analysis of regional neural stem/progenitor cells ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY The developing mammalian brain generates a variety of Neural Progenitor Cells ST:STUDY_SUMMARY (NPCs). Primary NPCs throughout the neuraxis are derived from the ventricular ST:STUDY_SUMMARY zone. Intermediate progenitor cells (IPCs) are produced uniquely in the ST:STUDY_SUMMARY telencephalon and contribute extensively to the neurons that comprise the ST:STUDY_SUMMARY cerebral cortex and basal ganglia. It is known that the fate of the diverse NPC ST:STUDY_SUMMARY populations is determined by the interplay of transcription factors and ST:STUDY_SUMMARY regulation by regional humoral cues. However, despite our recent appreciation ST:STUDY_SUMMARY that nutrient-regulated intracellular metabolic milieu (pO2, energy, and redox ST:STUDY_SUMMARY state) significantly influence cell fate, an unexplored area is whether NPCs ST:STUDY_SUMMARY have intrinsic metabolic identity, and if so, the mechanism by which molecular ST:STUDY_SUMMARY metabolism contributes to brain development.Little is known however, if ST:STUDY_SUMMARY intrinsic differences in cellular metabolism of regional NPCs make certain NPCs ST:STUDY_SUMMARY susceptible while others resistant to genetic and environmental insults. We ST:STUDY_SUMMARY conjectured that regional (fore-and hindbrain) NPCs are metabolically distinct. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0016334 S00026132 NPC source:forebrain SUBJECT_SAMPLE_FACTORS SU0016335 S00026133 NPC source:forebrain SUBJECT_SAMPLE_FACTORS SU0016336 S00026134 NPC source:hindbrain SUBJECT_SAMPLE_FACTORS SU0016337 S00026135 NPC source:hindbrain #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A011 - Glycolysis-TCA-nucleotides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg protein MS_METABOLITE_DATA_START Samples S00026132 S00026133 S00026134 S00026135 Factors NPC source:forebrain NPC source:forebrain NPC source:hindbrain NPC source:hindbrain PHOSPHOENOLPYRUVATE 3.886997781 5.398145468 4.059950455 3.092003803 D-ERYTHROSE 4-PHOSPHATE 20.34995022 14.79423862 25.53561269 18.74621727 BETA-NAD+ .299008893 .263429119 .804146524 .678213187 COENZYME II .637049112 .215437899 .178526499 .267162046 SEDOHEPTULOSE 7-PHOSPHATE 3.904039503 2.224604138 4.123699279 3.869276462 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 6.085621569 1.854668387 1.871040565 3.606827328 NADP-REDUCED 0 0 0 0 CITRIC ACID 48.5491184 11.58314121 23.44760015 20.52089241 NADH 19.23263829 .193968941 .553692434 .644093146 FAD .175350183 .169438275 .404843723 .398166614 MALIC ACID 10.70852479 16.72993796 43.66932609 48.47435831 ADENOSINE TRIPHOSPHATE 3.402329405 1.44722611 12.0012695 11.03019019 ROBISON ESTER 23.75964361 22.19374424 62.04572981 41.69657121 SUCROSE 4.800300233 4.142997676 5.892170226 9.778024279 ADENOSINE 5'-DIPHOSPHATE 3.766189115 1.433392119 19.00124464 24.66291966 ADENOSINE 5'-MONOPHOSPHATE 19.26190717 20.31379598 51.76080582 48.27363017 GLYCERATE 3-PHOSPHATE 47.59116421 41.81434839 44.03546965 41.88394658 RIBOSE PHOSPHATE 5.96044819 4.993082081 10.70262238 10.4523611 6-PHOSPHOGLUCONIC ACID 7.98731687 5.637812928 4.349344647 5.072449283 ACETOACETYL-COA 0 0 0 0 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PHOSPHOENOLPYRUVATE 1005 D-ERYTHROSE 4-PHOSPHATE 122357 BETA-NAD+ 15938971 COENZYME II 15938972 SEDOHEPTULOSE 7-PHOSPHATE 165007 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 NADP-REDUCED 22833512 CITRIC ACID 311 NADH 439153 FAD 46906035 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 ROBISON ESTER 5958 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 ACETOACETYL-COA 92153 METABOLITES_END #END