#METABOLOMICS WORKBENCH araskind_20170802_131251 DATATRACK_ID:1175 STUDY_ID:ST000828 ANALYSIS_ID:AN001834 PROJECT_ID:PR000219 VERSION 1 CREATED_ON January 15, 2019, 12:36 pm #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:DEPARTMENT Deaprtment of Pathology PR:LABORATORY Sem H. Phan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-7635731 #STUDY ST:STUDY_TITLE Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human ST:STUDY_TITLE IPF & Normal Lung Fiboblasts 3 ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis (especially interested in ST:STUDY_SUMMARY alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 6 ST:TOTAL_SUBJECTS 16 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0004492 S00015469 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004493 S00015470 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004494 S00015471 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004495 S00015472 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004496 S00015473 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004497 S00015474 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004498 S00015475 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004499 S00015476 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004500 S00015477 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004501 S00015478 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004502 S00015479 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004503 S00015480 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004504 S00015481 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004505 S00015482 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004506 S00015483 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004507 S00015484 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A010-Ceramides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Phenyl CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Pinnacle DB Biphenyl, 140A, 1.9mkm, 2.1mm X 50mm (Restek) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A010-Ceramides.pdf AN:ACQUISITION_PARAMETERS_FILE QM033_2mmC18_Ceramide_MRM #MS MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS none #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS counts MS_METABOLITE_DATA_START Samples S00015469 S00015470 S00015471 S00015472 S00015473 S00015474 S00015475 S00015476 S00015477 S00015478 S00015479 S00015480 S00015481 S00015482 S00015483 S00015484 Factors Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N C22 CER 5703936.582 5015879.938 5467201.523 6207717.35 6183899.084 6941223.742 1789750.106 2430036.041 2736578.926 1759028.005 2164439.651 1043406.914 835781.0316 1106883.454 1281598.917 1273825.67 C24:1 CER 22157790.11 20169886.19 28601422.45 38763327.08 33508656.7 27013966.14 4395266.064 5056058.144 5724159.406 3835527.436 4598070.547 3316148.601 2967606.983 4547323.576 4872113.069 4184632.31 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type C22 CER 5283567 C24:1 CER 5283568 METABOLITES_END #END