#METABOLOMICS WORKBENCH araskind_20170802_131251 DATATRACK_ID:1175 STUDY_ID:ST000828 ANALYSIS_ID:AN001835 PROJECT_ID:PR000219 VERSION 1 CREATED_ON January 15, 2019, 12:36 pm #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:DEPARTMENT Deaprtment of Pathology PR:LABORATORY Sem H. Phan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-7635731 #STUDY ST:STUDY_TITLE Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human ST:STUDY_TITLE IPF & Normal Lung Fiboblasts 3 ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis (especially interested in ST:STUDY_SUMMARY alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 6 ST:TOTAL_SUBJECTS 16 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0004492 S00015469 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004493 S00015470 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004494 S00015471 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004495 S00015472 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004496 S00015473 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004497 S00015474 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004498 S00015475 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004499 S00015476 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004500 S00015477 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004501 S00015478 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004502 S00015479 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004503 S00015480 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004504 S00015481 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004505 S00015482 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004506 S00015483 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004507 S00015484 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A010-Ceramides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Phenyl CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Pinnacle DB Biphenyl, 140A, 1.9mkm, 2.1mm X 50mm (Restek) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A010-Ceramides.pdf AN:ACQUISITION_PARAMETERS_FILE QM033_2mmC18_Ceramide_MRM #MS MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS none #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00015469 S00015470 S00015471 S00015472 S00015473 S00015474 S00015475 S00015476 S00015477 S00015478 S00015479 S00015480 S00015481 S00015482 S00015483 S00015484 Factors Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N C14 CER 13.18467456 11.3554499 16.71693126 17.5211094 14.99545025 16.52230241 4.341071808 4.523389516 3.910946385 3.72642603 3.686349582 2.567389296 2.579638735 3.295066866 3.169451064 2.82618964 N-(OLEOYL)-CERAMIDE 60.09308441 56.13348308 79.88984208 80.01543077 47.18888104 52.99986529 8.563245435 8.477443107 8.287113223 8.085358 8.100988521 7.322380042 7.567238928 7.561450824 7.569146526 7.309183262 N-PALMITOYLSPHINGOSINE 3589.962036 3102.803433 5239.218675 4368.8023 2968.105857 3291.572604 111.2430941 134.7340545 102.0941312 106.2026024 96.60617206 129.5525361 121.4395177 158.712242 153.9979781 138.7477852 N-STEAROYLSPHINGOSINE 541.0739629 408.8605058 688.209609 530.1515236 523.4719251 650.0436023 6.172734398 8.660236358 6.583264455 6.456199665 5.610454373 3.182783523 2.541264579 3.43938129 3.66297448 3.129887079 N-ICOSANOYLSPHINGOSINE 842.957053 842.3758328 982.6844946 833.68724 916.7110151 1578.309097 7.319720471 8.581557899 7.358170248 6.659164131 6.719343166 6.324317278 5.929982975 6.889094001 7.170088474 6.964446368 C24 CER 1279.74824 1379.284799 542.9248375 1478.376646 1147.90664 1505.891429 130.060554 159.1229516 308.3833215 112.5124306 292.9832778 32.53552938 30.83932337 37.78045285 41.90340042 35.88320168 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type C14 CER 5282310 N-(OLEOYL)-CERAMIDE 5283563 N-PALMITOYLSPHINGOSINE 5283564 N-STEAROYLSPHINGOSINE 5283565 N-ICOSANOYLSPHINGOSINE 5283566 C24 CER 5283571 METABOLITES_END #END