#METABOLOMICS WORKBENCH araskind_20170802_184646 DATATRACK_ID:1187 STUDY_ID:ST000836 ANALYSIS_ID:AN001339 PROJECT_ID:PR000596 VERSION 1 CREATED_ON August 9, 2017, 9:52 am #PROJECT PR:PROJECT_TITLE Lean Obese Human Glucose Metabolism PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Lean and obese skeletal muscle TCA plus response to OGTT PR:INSTITUTE Ohio University PR:DEPARTMENT Biomedical Sciences PR:LABORATORY Consitt Lab PR:LAST_NAME Consitt PR:FIRST_NAME Leslie PR:ADDRESS Athens, Ohio PR:EMAIL consitt@ohio.edu PR:PHONE 252-367-9228 #STUDY ST:STUDY_TITLE Pilot Human Muscle oral glucose tolerance test (OGTT) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Obesity is associated with metabolic inflexibility, the inability to switch from ST:STUDY_SUMMARY lipid to glucose oxidation during physiological conditions favoring excess ST:STUDY_SUMMARY glucose and insulin, which could contribute to the increased incidence of type 2 ST:STUDY_SUMMARY diabetes. Despite this knowledge, the role of skeletal muscle metabolites during ST:STUDY_SUMMARY these physiological conditions remain unclear and warrant further investigation. ST:STUDY_SUMMARY The purpose of this pilot study is to determine if: 1) there are differences in ST:STUDY_SUMMARY skeletal muscle glycolysis and TCA metabolites and amino acids between lean and ST:STUDY_SUMMARY obese individuals in the fasted state, and 2) if these metabolites change after ST:STUDY_SUMMARY 50 min of glucose ingestion, and 3) if there are differences in these ST:STUDY_SUMMARY metabolites between lean and obese individuals after 50 min of glucose ST:STUDY_SUMMARY ingestion. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 6 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0019613 S00028061 Body Comp:Lean | Gender:Female | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0019613 S00028062 Body Comp:Lean | Gender:Female | Timepoint:50min SUBJECT_SAMPLE_FACTORS SU0019614 S00028063 Body Comp:Lean | Gender:Female | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0019614 S00028064 Body Comp:Lean | Gender:Female | Timepoint:50min SUBJECT_SAMPLE_FACTORS SU0019615 S00028065 Body Comp:Obese | Gender:Female | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0019615 S00028066 Body Comp:Obese | Gender:Female | Timepoint:50min SUBJECT_SAMPLE_FACTORS SU0019616 S00028067 Body Comp:Obese | Gender:Female | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0019616 S00028068 Body Comp:Obese | Gender:Female | Timepoint:50min SUBJECT_SAMPLE_FACTORS SU0019617 S00028069 Body Comp:Lean | Gender:Female | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0019618 S00028070 Body Comp:Obese | Gender:Female | Timepoint:Baseline #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A043-TCA-Plus-20170713.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A043-TCA-Plus-20170713.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00028061 S00028062 S00028063 S00028064 S00028065 S00028066 S00028067 S00028068 S00028069 S00028070 Factors Body Comp:Lean | Gender:Female | Timepoint:Baseline Body Comp:Lean | Gender:Female | Timepoint:50min Body Comp:Lean | Gender:Female | Timepoint:Baseline Body Comp:Lean | Gender:Female | Timepoint:50min Body Comp:Obese | Gender:Female | Timepoint:Baseline Body Comp:Obese | Gender:Female | Timepoint:50min Body Comp:Obese | Gender:Female | Timepoint:Baseline Body Comp:Obese | Gender:Female | Timepoint:50min Body Comp:Lean | Gender:Female | Timepoint:Baseline Body Comp:Obese | Gender:Female | Timepoint:Baseline PHOSPHOENOLPYRUVATE .00213128 .001952572 .001018092 .000623999 .000801788 .001492632 .001002816 .000948342 .001708273 .000767851 D-ERYTHROSE 4-PHOSPHATE .12203673 .115307476 .126482161 .118342766 .051992602 .074629928 .083051331 .061730827 .122757198 .111914909 COENZYME II .000632701 .000547026 .000382718 .000199346 .000317612 .000317304 .00032002 .000370228 .000385569 .000278579 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) .003366351 .021479301 .003814524 .004759143 .004974825 .002320589 .002732335 .011600636 .052668641 .005712172 CITRIC ACID .002334123 .00271885 .002116017 .000389616 .001573469 .000903126 .000964798 .002162508 .0000465561 .000968461 NADH .000824558 .000898759 .000834729 .001808218 .000590548 FAD .0007391 .000615354 .000392966 .000234917 .000335183 .00034329 .000377112 .000540439 .000661625 .000328524 MALIC ACID .029181517 .023555064 .019282136 .032809597 .016214858 .027312525 .009975294 .012300151 .080148141 .026913984 ADENOSINE TRIPHOSPHATE .003013033 .008371785 .004155997 .004881207 .002007809 .005683393 .00299616 .014967599 .008340835 .00583805 ROBISON ESTER .145237678 .139315113 .156587551 .139901695 .062044801 .091301772 .11256106 .167120144 .156886935 .140506844 SUCROSE .003147867 .006290595 .005655238 .003730913 .005841245 .004119574 .005654122 .003045462 .012105958 .009566778 ADENOSINE 5'-DIPHOSPHATE .002445498 .004092243 .001924469 .001567872 .001191739 .003342891 .002994112 .005112023 .010152059 .002758972 ADENOSINE 5'-MONOPHOSPHATE .001058294 .000583199 .00038879 .000241391 .00030374 .000335125 .000989887 .000325938 .000533163 .000294118 GLYCERATE 3-PHOSPHATE .026719194 .054165394 .042736463 .014043179 .04169143 .03664765 .005766129 .025400285 .042661625 .021169164 RIBOSE PHOSPHATE .002874408 .00328537 .002460273 .002026962 .00642201 .004256272 .002409242 .002235516 .005198706 .002006937 6-PHOSPHOGLUCONIC ACID .001209242 .001047428 .000659666 .000363254 .000572441 .000533254 .000638889 .000449263 .000483601 .000477617 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PHOSPHOENOLPYRUVATE 1005 D-ERYTHROSE 4-PHOSPHATE 122357 COENZYME II 15938972 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 CITRIC ACID 311 NADH 439153 FAD 46906035 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 ROBISON ESTER 5958 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 METABOLITES_END #END