#METABOLOMICS WORKBENCH araskind_20170802_185111 DATATRACK_ID:1189 STUDY_ID:ST000838 ANALYSIS_ID:AN001342 PROJECT_ID:PR000598 VERSION 1 CREATED_ON August 9, 2017, 10:02 am #PROJECT PR:PROJECT_TITLE Obesity and marrow and MAT metabolomics Studies PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY MAT and bone marrow collected to evaluate lipid content/lipid type. In addition, PR:PROJECT_SUMMARY marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be PR:PROJECT_SUMMARY performed. PR:INSTITUTE University of Michigan PR:DEPARTMENT Pedatric Endocrinology PR:LABORATORY Singer Lab PR:LAST_NAME Singer PR:FIRST_NAME Kanakadurga PR:ADDRESS Ann Arbor, MI PR:EMAIL ksinger@umich.edu PR:PHONE 734-764-5175 #STUDY ST:STUDY_TITLE Obesity and marrow and marrow adipose tissue (MAT) metabolomics ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Male C57Bl6j mice were fed HFD for 16-20 weeks and marrow adipose tissue (MAT) ST:STUDY_SUMMARY and bone marrow collected to evaluate lipid content/lipid type. In addition, ST:STUDY_SUMMARY marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be ST:STUDY_SUMMARY performed. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 6 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0018085 S00028167 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018086 S00028168 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018087 S00028169 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018088 S00028170 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018089 S00028171 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018090 S00028172 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018085 S00028173 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018086 S00028174 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018087 S00028175 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018088 S00028176 Diet:HFD | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018089 S00028177 Diet:HFD | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018090 S00028178 Diet:HFD | Sample type:Bone Marrow #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A008_Acyl_Carnitines.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A008 - Acyl Carnitines.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00028173 S00028174 S00028175 S00028176 S00028177 S00028178 Factors Diet:ND | Sample type:Bone Marrow Diet:ND | Sample type:Bone Marrow Diet:ND | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow ACETYL-DL-CARNITINE 2.617587629 2.366129447 3.932355554 3.385854692 3.368573132 2.826828447 DECANOYLCARNITINE .003120276 .003134043 .004988492 .007888528 .008641933 .006227385 LAUROYLCARNITINE .010178748 .008716606 .011862037 .017448186 .017930409 .013095703 PROPIONYLCARNITINE .574179022 .393756096 .773290471 .603598169 .468154316 .327936557 L-PALMITOYLCARNITINE 1.040042758 .953084605 1.466614966 1.304384654 1.246244341 1.124786679 O-OCTANOYLCARNITINE .003345912 .002683524 .003953402 .006790042 .007924983 .005490354 BUTYRYLCARNITINE .170839748 .131013211 .219106914 .251036456 .27601018 .207004786 DL-CARNITINE 18.97298784 16.10546605 23.70815582 30.53023725 26.16806711 21.93564217 AC1MIY4G .048296266 .031647112 .036256988 .078118539 .065277801 .051786207 ARACHIDONYL CARNITINE .015967081 .013932509 .018808398 .040054642 .040279638 .031538205 LINOELAIDYL CARNITINE .069635233 .061573999 .087175219 .094809573 .090983847 .0803107 18:3 CARNITINE .005502427 .004602081 .006641747 .006608155 .007825748 .005580467 CIS-4-DECENOYL CARNITINE .001134437 .000786245 .001002725 .002391534 .002049489 .001607993 ISOVALERYLCARNITINE .054846724 .043896945 .071195469 .135932149 .176741207 .094831225 MYRISTOYLCARNITINE .146793066 .136523451 .182005125 .176117853 .165735046 .135898904 OLEOYLCARNITINE .401028911 .381109278 .512141994 .571891531 .536854588 .428065248 GLUTARYLCARNITINE .048288153 .041427163 .052358271 .071808094 .07851578 .060419238 3-HYDROXYLAUROYLCARNITINE .028330219 .023915653 .033418936 .042067107 .036095193 .028339082 3-HYDROXYMYRISTOYLCARNITINE .048480453 .043385551 .066417795 .072370925 .068469389 .057786238 3-HYDROXYPALMITOYLCARNITINE .134710147 .106075839 .183682182 .228292461 .214198846 .185891878 3-HYDROXYLINOLEYLCARNITINE .068212881 .055489851 .087530019 .124826798 .11274007 .100834056 16:1 CARNITINE .082143363 .084540849 .113158662 .110403289 .105039671 .088205324 8:1 CARNITINE .001012211 .000740456 .001074 .003859111 .004007355 .003010326 14:2 CARNITINE .001188135 .000912889 .00135654 .002923463 .002520837 .00210256 12:1 CARNITINE .000867518 .000852069 .001235516 .00227291 .002036183 .001662023 14:1 CARNITINE .010597757 .009976649 .012602283 .019903137 .01972405 .015658714 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type ACETYL-DL-CARNITINE 1 DECANOYLCARNITINE 10245190 LAUROYLCARNITINE 10427569 PROPIONYLCARNITINE 107738 L-PALMITOYLCARNITINE 11953816 O-OCTANOYLCARNITINE 123701 BUTYRYLCARNITINE 213144 DL-CARNITINE 288 AC1MIY4G 3082079 ARACHIDONYL CARNITINE 53477832 LINOELAIDYL CARNITINE 53477834 18:3 CARNITINE 54500892 CIS-4-DECENOYL CARNITINE 57357170 ISOVALERYLCARNITINE 6426851 MYRISTOYLCARNITINE 6426854 OLEOYLCARNITINE 6441392 GLUTARYLCARNITINE 71317118 3-HYDROXYLAUROYLCARNITINE 71464535 3-HYDROXYMYRISTOYLCARNITINE 71464541 3-HYDROXYPALMITOYLCARNITINE 71464553 3-HYDROXYLINOLEYLCARNITINE 71464556 16:1 CARNITINE 76040365 8:1 CARNITINE 77908888 14:2 CARNITINE 87087940 12:1 CARNITINE 87087953 14:1 CARNITINE 87088002 METABOLITES_END #END