#METABOLOMICS WORKBENCH hormel101_20170810_132003_mwtab.txt DATATRACK_ID:1205 STUDY_ID:ST000869 ANALYSIS_ID:AN001399 PROJECT_ID:PR000575 VERSION 1 CREATED_ON August 28, 2017, 10:41 am #PROJECT PR:PROJECT_TITLE Mayo Pilot and Feasiblity: Targeting Myelin Metabolism of Kallikrein 6 Signals PR:PROJECT_TITLE through PAR1 and PAR2 to Enchance Recovery of Function after SCI PR:PROJECT_SUMMARY CNS trauma generates a proteolytic imbalance contributing to secondary injury, PR:PROJECT_SUMMARY including axonopathy and neuron degeneration. Kallikrein 6 (Klk6) is a serine PR:PROJECT_SUMMARY protease implicated in neurodegeneration and here we investigate the role of PR:PROJECT_SUMMARY protease activated receptors 1 (PAR1) and PAR2 in mediating these effects in PR:PROJECT_SUMMARY mice with spinal cord injury (SCI). This project conducts untargeted Profiling PR:PROJECT_SUMMARY (unbiased metabolomics assay) and targeted lipid analysis. The lipid assays are PR:PROJECT_SUMMARY 1) free fatty acid composition of lipids; 2) free fatty acid panel; 3) PR:PROJECT_SUMMARY cholesterol concentration (free and bound); 4) Ceramides, including galactosyl PR:PROJECT_SUMMARY and glucosyl; 5) sphingomyelin. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Scarisbrick PR:FIRST_NAME Isobel PR:ADDRESS 200 First Street SW, Rochester, MN 55905 PR:EMAIL scarisbrick.isobel@mayo.edu PR:PHONE 507-284-0124 #STUDY ST:STUDY_TITLE Large Untargeted Profiling in Kallikrein 6 Mice after SCI ST:STUDY_SUMMARY Large untargeted profiling mouse myelin of Kallikrein 6 Signals through PAR1 and ST:STUDY_SUMMARY PAR2 to Enchance Recovery of Function after SCI. The samples submitted are ST:STUDY_SUMMARY purified myelin preparations from the postnatal day 21, 60, or 90 mouse spinal ST:STUDY_SUMMARY cord (SC). There are 12 samples total in Project 3, n=3 for K6+/+ or K6-/- at ST:STUDY_SUMMARY either P21 or P90. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Scarisbrick ST:FIRST_NAME Isobel ST:ADDRESS 200 First Street SW, Rochester, MN 55905 ST:EMAIL scarisbrick.isobel@mayo.edu ST:PHONE 507-284-0124 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS MS6020-1 nC18-8jun16-001-r001_1 Group:K6-/- | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-1 nC18-8jun16-001-r002_1 Group:K6-/- | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-2 nC18-8jun16-002-r001_1 Group:K6-/- | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-2 nC18-8jun16-002-r002_1 Group:K6-/- | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-3 nC18-8jun16-003-r001_1 Group:K6+/+ | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-3 nC18-8jun16-003-r002_1 Group:K6+/+ | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-4 nC18-8jun16-004-r001_1 Group:K6+/+ | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-4 nC18-8jun16-004-r002_1 Group:K6+/+ | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-5 nC18-8jun16-005-r001_1 Group:K6-/- | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-5 nC18-8jun16-005-r002_1 Group:K6-/- | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-6 nC18-8jun16-006-r001_1 Group:K6-/- | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-6 nC18-8jun16-006-r002_1 Group:K6-/- | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-7 nC18-8jun16-007-r001_1 Group:K6+/+ | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-7 nC18-8jun16-007-r002_1 Group:K6+/+ | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-8 nC18-8jun16-008-r001_1 Group:K6+/+ | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-8 nC18-8jun16-008-r002_1 Group:K6+/+ | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-9 nC18-8jun16-009-r001_1 Group:K6-/- | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-9 nC18-8jun16-009-r002_1 Group:K6-/- | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-10 nC18-8jun16-010-r001_1 Group:K6+/+ | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-10 nC18-8jun16-010-r002_1 Group:K6+/+ | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-11 nC18-8jun16-011-r001_1 Group:K6+/+ | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-11 nC18-8jun16-011-r002_1 Group:K6+/+ | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-12 nC18-8jun16-012-r001_1 Group:K6-/- | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-12 nC18-8jun16-012-r002_1 Group:K6-/- | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-13 nC18-8jun16-013-r001_1 Group:K6-/- | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-13 nC18-8jun16-013-r002_1 Group:K6-/- | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-14 nC18-8jun16-014-r001_1 Group:K6+/+ | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-14 nC18-8jun16-014-r002_1 Group:K6+/+ | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-15 nC18-8jun16-015-r001_1 Group:K6+/+ | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-15 nC18-8jun16-015-r002_1 Group:K6+/+ | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-16 nC18-8jun16-016-r001_1 Group:K6-/- | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-16 nC18-8jun16-016-r002_1 Group:K6-/- | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-17 nC18-8jun16-017-r001_1 Group:K6-/- | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-17 nC18-8jun16-017-r002_1 Group:K6-/- | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-18 nC18-8jun16-018-r001_1 Group:K6+/+ | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-18 nC18-8jun16-018-r002_1 Group:K6+/+ | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-19 nC18-8jun16-019-r001_1 Group:K6+/+ | Time point:P90 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-19 nC18-8jun16-019-r002_1 Group:K6+/+ | Time point:P90 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-20 nC18-8jun16-020-r001_1 Group:K6-/- | Time point:P21 | rep:1 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-20 nC18-8jun16-020-r002_1 Group:K6-/- | Time point:P21 | rep:2 type=NC18 SUBJECT_SAMPLE_FACTORS MS6020-1 pC18-7jun16-001-r001 Group:K6-/- | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-1 pC18-7jun16-001-r002 Group:K6-/- | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-2 pC18-7jun16-002-r001 Group:K6-/- | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-2 pC18-7jun16-002-r002 Group:K6-/- | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-3 pC18-7jun16-003-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-3 pC18-7jun16-003-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-4 pC18-7jun16-004-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-4 pC18-7jun16-004-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-5 pC18-7jun16-005-r001 Group:K6-/- | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-5 pC18-7jun16-005-r002 Group:K6-/- | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-6 pC18-7jun16-006-r001 Group:K6-/- | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-6 pC18-7jun16-006-r002 Group:K6-/- | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-7 pC18-7jun16-007-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-7 pC18-7jun16-007-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-8 pC18-7jun16-008-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-8 pC18-7jun16-008-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-9 pC18-7jun16-009-r001 Group:K6-/- | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-9 pC18-7jun16-009-r002 Group:K6-/- | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-10 pC18-7jun16-010-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-10 pC18-7jun16-010-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-11 pC18-7jun16-011-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-11 pC18-7jun16-011-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-12 pC18-7jun16-012-r001 Group:K6-/- | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-12 pC18-7jun16-012-r002 Group:K6-/- | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-13 pC18-7jun16-013-r001 Group:K6-/- | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-13 pC18-7jun16-013-r002 Group:K6-/- | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-14 pC18-7jun16-014-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-14 pC18-7jun16-014-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-15 pC18-7jun16-015-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-15 pC18-7jun16-015-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-16 pC18-7jun16-016-r001 Group:K6-/- | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-16 pC18-7jun16-016-r002 Group:K6-/- | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-17 pC18-7jun16-017-r001 Group:K6-/- | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-17 pC18-7jun16-017-r002 Group:K6-/- | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-18 pC18-7jun16-018-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-18 pC18-7jun16-018-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-19 pC18-7jun16-019-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-19 pC18-7jun16-019-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-20 pC18-7jun16-020-r001 Group:K6-/- | Time point:P21 | rep:1 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-20 pC18-7jun16-020-r002 Group:K6-/- | Time point:P21 | rep:2 type=PC18 SUBJECT_SAMPLE_FACTORS MS6020-1 nhilic-9jun16-001-r001 Group:K6-/- | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-1 nhilic-9jun16-001-r002 Group:K6-/- | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-2 nhilic-9jun16-002-r001 Group:K6-/- | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-2 nhilic-9jun16-002-r002 Group:K6-/- | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-3 nhilic-9jun16-003-r001 Group:K6+/+ | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-3 nhilic-9jun16-003-r002 Group:K6+/+ | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-4 nhilic-9jun16-004-r001 Group:K6+/+ | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-4 nhilic-9jun16-004-r002 Group:K6+/+ | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-5 nhilic-9jun16-005-r001 Group:K6-/- | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-5 nhilic-9jun16-005-r002 Group:K6-/- | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-6 nhilic-9jun16-006-r001 Group:K6-/- | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-6 nhilic-9jun16-006-r002 Group:K6-/- | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-7 nhilic-9jun16-007-r001 Group:K6+/+ | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-7 nhilic-9jun16-007-r002 Group:K6+/+ | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-8 nhilic-9jun16-008-r001 Group:K6+/+ | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-8 nhilic-9jun16-008-r002 Group:K6+/+ | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-9 nhilic-9jun16-009-r001 Group:K6-/- | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-9 nhilic-9jun16-009-r002 Group:K6-/- | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-10 nhilic-9jun16-010-r001 Group:K6+/+ | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-10 nhilic-9jun16-010-r002 Group:K6+/+ | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-11 nhilic-9jun16-011-r001 Group:K6+/+ | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-11 nhilic-9jun16-011-r002 Group:K6+/+ | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-12 nhilic-9jun16-012-r001 Group:K6-/- | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-12 nhilic-9jun16-012-r002 Group:K6-/- | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-13 nhilic-9jun16-013-r001 Group:K6-/- | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-13 nhilic-9jun16-013-r002 Group:K6-/- | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-14 nhilic-9jun16-014-r001 Group:K6+/+ | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-14 nhilic-9jun16-014-r002 Group:K6+/+ | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-15 nhilic-9jun16-015-r001 Group:K6+/+ | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-15 nhilic-9jun16-015-r002 Group:K6+/+ | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-16 nhilic-9jun16-016-r001 Group:K6-/- | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-16 nhilic-9jun16-016-r002 Group:K6-/- | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-17 nhilic-9jun16-017-r001 Group:K6-/- | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-17 nhilic-9jun16-017-r002 Group:K6-/- | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-18 nhilic-9jun16-018-r001 Group:K6+/+ | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-18 nhilic-9jun16-018-r002 Group:K6+/+ | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-19 nhilic-9jun16-019-r001 Group:K6+/+ | Time point:P90 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-19 nhilic-9jun16-019-r002 Group:K6+/+ | Time point:P90 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-20 nhilic-9jun16-020-r001 Group:K6-/- | Time point:P21 | rep:1 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-20 nhilic-9jun16-020-r002 Group:K6-/- | Time point:P21 | rep:2 type=NHILIC SUBJECT_SAMPLE_FACTORS MS6020-1 philic-7jun16-001-r001 Group:K6-/- | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-1 philic-7jun16-001-r002 Group:K6-/- | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-2 philic-7jun16-002-r001 Group:K6-/- | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-2 philic-7jun16-002-r002 Group:K6-/- | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-3 philic-7jun16-003-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-3 philic-7jun16-003-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-4 philic-7jun16-004-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-4 philic-7jun16-004-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-5 philic-7jun16-005-r001 Group:K6-/- | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-5 philic-7jun16-005-r002 Group:K6-/- | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-6 philic-7jun16-006-r001 Group:K6-/- | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-6 philic-7jun16-006-r002 Group:K6-/- | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-7 philic-7jun16-007-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-7 philic-7jun16-007-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-8 philic-7jun16-008-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-8 philic-7jun16-008-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-9 philic-7jun16-009-r001 Group:K6-/- | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-9 philic-7jun16-009-r002 Group:K6-/- | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-10 philic-7jun16-010-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-10 philic-7jun16-010-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-11 philic-7jun16-011-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-11 philic-7jun16-011-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-12 philic-7jun16-012-r001 Group:K6-/- | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-12 philic-7jun16-012-r002 Group:K6-/- | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-13 philic-7jun16-013-r001 Group:K6-/- | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-13 philic-7jun16-013-r002 Group:K6-/- | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-14 philic-7jun16-014-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-14 philic-7jun16-014-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-15 philic-7jun16-015-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-15 philic-7jun16-015-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-16 philic-7jun16-016-r001 Group:K6-/- | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-16 philic-7jun16-016-r002 Group:K6-/- | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-17 philic-7jun16-017-r001 Group:K6-/- | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-17 philic-7jun16-017-r002 Group:K6-/- | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-18 philic-7jun16-018-r001 Group:K6+/+ | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-18 philic-7jun16-018-r002 Group:K6+/+ | Time point:P21 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-19 philic-7jun16-019-r001 Group:K6+/+ | Time point:P90 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-19 philic-7jun16-019-r002 Group:K6+/+ | Time point:P90 | rep:2 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-20 philic-7jun16-020-r001 Group:K6-/- | Time point:P21 | rep:1 type=PHILIC SUBJECT_SAMPLE_FACTORS MS6020-20 philic-7jun16-020-r002 Group:K6-/- | Time point:P21 | rep:2 type=PHILIC #COLLECTION CO:COLLECTION_SUMMARY Tissue is from adult mouse spinal cord (SC). We are submitting these samples for CO:COLLECTION_SUMMARY Untargeted Profiling (unbiased metabolomics assay) and for lipid analysis. The CO:COLLECTION_SUMMARY lipid assays we request are 1) free fatty acid composition of lipids; 2) free CO:COLLECTION_SUMMARY fatty acid panel; 3) cholesterol concentration (free and bound); 4) Ceramides, CO:COLLECTION_SUMMARY including galactosyl and glucosyl; 5) sphingomyelin. The Untargeted profiling is CO:COLLECTION_SUMMARY our top priority, followed by the lipid assays as listed. All samples were snap CO:COLLECTION_SUMMARY frozen at the point of harvest and approximate weights are provided. The samples CO:COLLECTION_SUMMARY are submitted as intact pieces of tissue. There are two different genotypes CO:COLLECTION_SUMMARY (K6+/+ and K6-/-) and 20 samples total, n=5 for each group that includes CO:COLLECTION_SUMMARY P21(K6+/+); P90 (K6+/+); P21 (K6-/-); and P90 (K6-/-). We would like to make CO:COLLECTION_SUMMARY comparisons across the 4 groups. #TREATMENT TR:TREATMENT_SUMMARY A 3g Clip produces moderate SCI including demyelination and clinical impairment TR:TREATMENT_SUMMARY and we recently published a detailed methodology. At 1 week after injury, the 3g TR:TREATMENT_SUMMARY injured mice are expected to have an average Basso Mouse Scale score (BMS)=5 on TR:TREATMENT_SUMMARY a 9 point scale such that they have frequent plantar stepping with no or some TR:TREATMENT_SUMMARY coordination. This level of impairment was chosen to provide a sufficient window TR:TREATMENT_SUMMARY to observe recovery. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY "large scale profiling of mouse spinal cord Lipids will be quantified in myelin SP:SAMPLEPREP_SUMMARY isolated in high yield and purity by subcellular fractionation from the SP:SAMPLEPREP_SUMMARY lumbosacral spinal cord. While there are no absolutely ‘myelin-specific’ SP:SAMPLEPREP_SUMMARY lipids, galactocerebroside is the most typical of myelin in the adult nervous SP:SAMPLEPREP_SUMMARY system being directly proportional to the amount of myelin. Sulfatide is another SP:SAMPLEPREP_SUMMARY galactolipid enriched in myelin. Together with cholesterol, these form 78% of SP:SAMPLEPREP_SUMMARY the total amount of lipid in the myelin membrane and each will be quantified SP:SAMPLEPREP_SUMMARY using LC/MS/MS. A highly sensitive assay for galactocerebroside was recently SP:SAMPLEPREP_SUMMARY established by the Mayo Metabolomics Core and can be implemented immediately. SP:SAMPLEPREP_SUMMARY The LC/MS/MS panel for free fatty acids, including the very long chain fatty SP:SAMPLEPREP_SUMMARY acids found in myelin is also routinely performed by the Core. Cholesterol will SP:SAMPLEPREP_SUMMARY be quantified using an NMR-based approach by the Mayo Dept. of Laboratory SP:SAMPLEPREP_SUMMARY Medicine Clinical Core. Additionally, we have a plan in place with the SP:SAMPLEPREP_SUMMARY Metabolomics Core to develop LC/MS/MS assays for sulfatide and sphingomyelin SP:SAMPLEPREP_SUMMARY during the Pilot proposal. Having quantitative assays for each of these key SP:SAMPLEPREP_SUMMARY myelin lipids will facilitate our goal to comprehensively profile myelin lipid SP:SAMPLEPREP_SUMMARY metabolism and will form foundational assays for a future NIH grant focused on SP:SAMPLEPREP_SUMMARY myelin metabolism." #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Waters Acquity HSS C18 (150 x 2.1mm, 1.8um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6550 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_RESULTS_FILE ST000869_AN001399_Results.txt UNITS:intensity #END