#METABOLOMICS WORKBENCH efahy_20180114_111150 DATATRACK_ID:1297 STUDY_ID:ST000915 ANALYSIS_ID:AN001486 PROJECT_ID:PR000633 VERSION 1 CREATED_ON January 14, 2018, 11:41 am #PROJECT PR:PROJECT_TITLE Biomarkers of NAFLD progression: a lipidomics approach to an epidemic PR:PROJECT_TYPE Lipidomics Study PR:PROJECT_SUMMARY The spectrum of nonalcoholic fatty liver disease (NAFLD) includes steatosis, PR:PROJECT_SUMMARY nonalcoholic steatohepatitis (NASH), and cirrhosis. Recognition and timely PR:PROJECT_SUMMARY diagnosis of these different stages, particularly NASH, is important for both PR:PROJECT_SUMMARY potential reversibility and limitation of complications. Liver biopsy remains PR:PROJECT_SUMMARY the clinical standard for definitive diagnosis. Diagnostic tools minimizing the PR:PROJECT_SUMMARY need for invasive procedures or that add information to histologic data are PR:PROJECT_SUMMARY important in novel management strategies for the growing epidemic of NAFLD. We PR:PROJECT_SUMMARY describe an 'omics' approach to detecting a reproducible signature of lipid PR:PROJECT_SUMMARY metabolites, aqueous intracellular metabolites, SNPs, and mRNA transcripts in a PR:PROJECT_SUMMARY double-blinded study of patients with different stages of NAFLD that involves PR:PROJECT_SUMMARY profiling liver biopsies, plasma, and urine samples. Using linear discriminant PR:PROJECT_SUMMARY analysis, a panel of 20 plasma metabolites that includes glycerophospholipids, PR:PROJECT_SUMMARY sphingolipids, sterols, and various aqueous small molecular weight components PR:PROJECT_SUMMARY involved in cellular metabolic pathways, can be used to differentiate between PR:PROJECT_SUMMARY NASH and steatosis. This identification of differential biomolecular signatures PR:PROJECT_SUMMARY has the potential to improve clinical diagnosis and facilitate therapeutic PR:PROJECT_SUMMARY intervention of NAFLD. PR:INSTITUTE University of California, San Diego PR:DEPARTMENT Bioengineering PR:LAST_NAME Fahy PR:FIRST_NAME Eoin PR:ADDRESS 9500 Gilman, La Jolla, CA, 92093, USA PR:EMAIL efahy@ucsd.edu PR:PHONE 858-534-4076 PR:FUNDING_SOURCE NIGMS Grant GM U54069338 PR:PUBLICATIONS https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340319/ PR:CONTRIBUTORS LIPID MAPS Consortium #STUDY ST:STUDY_TITLE Biomarkers of NAFLD progression: a lipidomics approach to an epidemic. Part ST:STUDY_TITLE 1:Liver ST:STUDY_TYPE Lipidomics Study ST:STUDY_SUMMARY The spectrum of nonalcoholic fatty liver disease (NAFLD) includes steatosis, ST:STUDY_SUMMARY nonalcoholic steatohepatitis (NASH), and cirrhosis. Recognition and timely ST:STUDY_SUMMARY diagnosis of these different stages, particularly NASH, is important for both ST:STUDY_SUMMARY potential reversibility and limitation of complications. Liver biopsy remains ST:STUDY_SUMMARY the clinical standard for definitive diagnosis. Diagnostic tools minimizing the ST:STUDY_SUMMARY need for invasive procedures or that add information to histologic data are ST:STUDY_SUMMARY important in novel management strategies for the growing epidemic of NAFLD. We ST:STUDY_SUMMARY describe an 'omics' approach to detecting a reproducible signature of lipid ST:STUDY_SUMMARY metabolites, aqueous intracellular metabolites, SNPs, and mRNA transcripts in a ST:STUDY_SUMMARY double-blinded study of patients with different stages of NAFLD that involves ST:STUDY_SUMMARY profiling liver biopsies, plasma, and urine samples. Using linear discriminant ST:STUDY_SUMMARY analysis, a panel of 20 plasma metabolites that includes glycerophospholipids, ST:STUDY_SUMMARY sphingolipids, sterols, and various aqueous small molecular weight components ST:STUDY_SUMMARY involved in cellular metabolic pathways, can be used to differentiate between ST:STUDY_SUMMARY NASH and steatosis. This identification of differential biomolecular signatures ST:STUDY_SUMMARY has the potential to improve clinical diagnosis and facilitate therapeutic ST:STUDY_SUMMARY intervention of NAFLD. ST:INSTITUTE University of California, San Diego ST:DEPARTMENT Bioengineering ST:LAST_NAME Fahy ST:FIRST_NAME Eoin ST:ADDRESS 9500 Gilman, La Jolla, CA, 92093, USA ST:EMAIL efahy@ucsd.edu ST:PHONE 858-534-4076 ST:PUBLICATIONS https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340319/ #SUBJECT SU:SUBJECT_TYPE Human clinical study SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 23-83 SU:GENDER Male and Female SU:HUMAN_ETHNICITY Black/White #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - NASH005 Diagnosis:Cirrhosis GENDER=M; BMI=31; ETHNICITY=White; AGE=45; AST=55; ALT=20; ALKP=171; TBIL=8.2; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH007 Diagnosis:Cirrhosis GENDER=F; BMI=40.3; ETHNICITY=White; AGE=64; AST=39; ALT=20; ALKP=102; TBIL=3; GLUCOSE=92 SUBJECT_SAMPLE_FACTORS - NASH009 Diagnosis:Cirrhosis GENDER=M; BMI=40; ETHNICITY=White; AGE=49; AST=162; ALT=66; ALKP=123; TBIL=10.5; GLUCOSE=119 SUBJECT_SAMPLE_FACTORS - NASH013 Diagnosis:Cirrhosis GENDER=M; BMI=35; ETHNICITY=White; AGE=59; AST=44; ALT=32; ALKP=95; TBIL=2.9; GLUCOSE=100 SUBJECT_SAMPLE_FACTORS - NASH016 Diagnosis:Cirrhosis GENDER=M; BMI=26; ETHNICITY=Asian; AGE=66; AST=223; ALT=100; ALKP=149; TBIL=5.2; GLUCOSE=117 SUBJECT_SAMPLE_FACTORS - NASH022 Diagnosis:Cirrhosis GENDER=M; BMI=27; ETHNICITY=White; AGE=63; AST=55; ALT=34; ALKP=91; TBIL=6.5; GLUCOSE=220 SUBJECT_SAMPLE_FACTORS - NASH026 Diagnosis:Cirrhosis GENDER=M; BMI=26.5; ETHNICITY=White; AGE=57; AST=18; ALT=38; ALKP=37; TBIL=4.2; GLUCOSE=122 SUBJECT_SAMPLE_FACTORS - NASH027 Diagnosis:Cirrhosis GENDER=M; BMI=31; ETHNICITY=-; AGE=58; AST=30; ALT=36; ALKP=105; TBIL=3.4; GLUCOSE=131 SUBJECT_SAMPLE_FACTORS - NASH028 Diagnosis:Cirrhosis GENDER=F; BMI=31; ETHNICITY=White; AGE=48; AST=46; ALT=47; ALKP=192; TBIL=2.4; GLUCOSE=173 SUBJECT_SAMPLE_FACTORS - NASH029 Diagnosis:Cirrhosis GENDER=F; BMI=21.5; ETHNICITY=White; AGE=49; AST=92; ALT=54; ALKP=277; TBIL=41.7; GLUCOSE=113 SUBJECT_SAMPLE_FACTORS - NASH040 Diagnosis:Cirrhosis GENDER=F; BMI=44; ETHNICITY=White; AGE=63; AST=36; ALT=26; ALKP=62; TBIL=1.7; GLUCOSE=65 SUBJECT_SAMPLE_FACTORS - NASH047 Diagnosis:Cirrhosis GENDER=M; BMI=-; ETHNICITY=White; AGE=60; AST=51; ALT=24; ALKP=57; TBIL=11.3; GLUCOSE=241 SUBJECT_SAMPLE_FACTORS - NASH048 Diagnosis:Cirrhosis GENDER=M; BMI=26; ETHNICITY=White; AGE=66; AST=44; ALT=23; ALKP=100; TBIL=4.5; GLUCOSE=79 SUBJECT_SAMPLE_FACTORS - NASH049 Diagnosis:Cirrhosis GENDER=M; BMI=35; ETHNICITY=White; AGE=71; AST=42; ALT=21; ALKP=82; TBIL=3.8; GLUCOSE=145 SUBJECT_SAMPLE_FACTORS - NASH052 Diagnosis:Cirrhosis GENDER=M; BMI=-; ETHNICITY=-; AGE=63; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH055 Diagnosis:Cirrhosis GENDER=M; BMI=36.8; ETHNICITY=White; AGE=67; AST=35; ALT=24; ALKP=91; TBIL=0.9; GLUCOSE=135 SUBJECT_SAMPLE_FACTORS - NASH064 Diagnosis:Cirrhosis GENDER=F; BMI=27.9; ETHNICITY=White; AGE=67; AST=67; ALT=31; ALKP=113; TBIL=5.5; GLUCOSE=82 SUBJECT_SAMPLE_FACTORS - NASH065 Diagnosis:Cirrhosis GENDER=M; BMI=41.9; ETHNICITY=White; AGE=36; AST=32; ALT=17; ALKP=44; TBIL=0.2; GLUCOSE=91 SUBJECT_SAMPLE_FACTORS - NASH068 Diagnosis:Cirrhosis GENDER=M; BMI=23; ETHNICITY=White; AGE=55; AST=46; ALT=21; ALKP=67; TBIL=1.4; GLUCOSE=96 SUBJECT_SAMPLE_FACTORS - NASH069 Diagnosis:Cirrhosis GENDER=M; BMI=29.9; ETHNICITY=White; AGE=56; AST=74; ALT=57; ALKP=258; TBIL=3.1; GLUCOSE=84 SUBJECT_SAMPLE_FACTORS - NASH010 Diagnosis:NASH GENDER=F; BMI=43.3; ETHNICITY=White; AGE=46; AST=33; ALT=35; ALKP=71; TBIL=0.4; GLUCOSE=175 SUBJECT_SAMPLE_FACTORS - NASH012 Diagnosis:NASH GENDER=F; BMI=49; ETHNICITY=White; AGE=38; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=89 SUBJECT_SAMPLE_FACTORS - NASH015 Diagnosis:NASH GENDER=M; BMI=44; ETHNICITY=White; AGE=58; AST=27; ALT=39; ALKP=41; TBIL=0.2; GLUCOSE=125 SUBJECT_SAMPLE_FACTORS - NASH018 Diagnosis:NASH GENDER=F; BMI=51; ETHNICITY=Black; AGE=44; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=96 SUBJECT_SAMPLE_FACTORS - NASH019 Diagnosis:NASH GENDER=M; BMI=34; ETHNICITY=White; AGE=73; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=107 SUBJECT_SAMPLE_FACTORS - NASH021 Diagnosis:NASH GENDER=M; BMI=28; ETHNICITY=White; AGE=59; AST=30; ALT=39; ALKP=63; TBIL=0.9; GLUCOSE=185 SUBJECT_SAMPLE_FACTORS - NASH030 Diagnosis:NASH GENDER=F; BMI=41.6; ETHNICITY=White; AGE=28; AST=24; ALT=24; ALKP=77; TBIL=0.1; GLUCOSE=89 SUBJECT_SAMPLE_FACTORS - NASH031 Diagnosis:NASH GENDER=F; BMI=41.5; ETHNICITY=White; AGE=46; AST=17; ALT=23; ALKP=94; TBIL=0.2; GLUCOSE=135 SUBJECT_SAMPLE_FACTORS - NASH035 Diagnosis:NASH GENDER=M; BMI=74.7; ETHNICITY=White; AGE=45; AST=23; ALT=30; ALKP=72; TBIL=0.3; GLUCOSE=84 SUBJECT_SAMPLE_FACTORS - NASH037 Diagnosis:NASH GENDER=M; BMI=44; ETHNICITY=White; AGE=53; AST=52; ALT=83; ALKP=98; TBIL=0.3; GLUCOSE=165 SUBJECT_SAMPLE_FACTORS - NASH038 Diagnosis:NASH GENDER=M; BMI=45; ETHNICITY=White; AGE=65; AST=36; ALT=37; ALKP=67; TBIL=0.3; GLUCOSE=97 SUBJECT_SAMPLE_FACTORS - NASH039 Diagnosis:NASH GENDER=M; BMI=48; ETHNICITY=White; AGE=35; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH044 Diagnosis:NASH GENDER=M; BMI=61; ETHNICITY=Black; AGE=41; AST=22; ALT=24; ALKP=57; TBIL=0.6; GLUCOSE=89 SUBJECT_SAMPLE_FACTORS - NASH057 Diagnosis:NASH GENDER=F; BMI=47.3; ETHNICITY=White; AGE=53; AST=25; ALT=18; ALKP=71; TBIL=0.6; GLUCOSE=99 SUBJECT_SAMPLE_FACTORS - NASH072 Diagnosis:NASH GENDER=M; BMI=77.3; ETHNICITY=White; AGE=50; AST=29; ALT=12; ALKP=85; TBIL=0.8; GLUCOSE=207 SUBJECT_SAMPLE_FACTORS - NASH074 Diagnosis:NASH GENDER=F; BMI=31; ETHNICITY=White; AGE=52; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=169 SUBJECT_SAMPLE_FACTORS - NASH084 Diagnosis:NASH GENDER=F; BMI=63; ETHNICITY=White; AGE=48; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=120 SUBJECT_SAMPLE_FACTORS - NASH087 Diagnosis:NASH GENDER=F; BMI=52; ETHNICITY=Black; AGE=58; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=175 SUBJECT_SAMPLE_FACTORS - NASH088 Diagnosis:NASH GENDER=F; BMI=40; ETHNICITY=Black; AGE=27; AST=15; ALT=19; ALKP=134; TBIL=0.4; GLUCOSE=133 SUBJECT_SAMPLE_FACTORS - NASH090 Diagnosis:NASH GENDER=F; BMI=35; ETHNICITY=White; AGE=32; AST=15; ALT=20; ALKP=69; TBIL=-; GLUCOSE=110 SUBJECT_SAMPLE_FACTORS - NASH003 Diagnosis:Normal GENDER=F; BMI=47.3; ETHNICITY=White; AGE=46; AST=36; ALT=54; ALKP=76; TBIL=-; GLUCOSE=90 SUBJECT_SAMPLE_FACTORS - NASH004 Diagnosis:Normal GENDER=M; BMI=-; ETHNICITY=White; AGE=23; AST=106; ALT=95; ALKP=-; TBIL=0.7; GLUCOSE=97 SUBJECT_SAMPLE_FACTORS - NASH006 Diagnosis:Normal GENDER=F; BMI=-; ETHNICITY=White; AGE=40; AST=68; ALT=72; ALKP=74; TBIL=0.5; GLUCOSE=93 SUBJECT_SAMPLE_FACTORS - NASH011 Diagnosis:Normal GENDER=M; BMI=29.1; ETHNICITY=White; AGE=54; AST=40; ALT=22; ALKP=42; TBIL=0.9; GLUCOSE=101 SUBJECT_SAMPLE_FACTORS - NASH014 Diagnosis:Normal GENDER=F; BMI=36; ETHNICITY=Black; AGE=63; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=139 SUBJECT_SAMPLE_FACTORS - NASH017 Diagnosis:Normal GENDER=M; BMI=45; ETHNICITY=White; AGE=59; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=101 SUBJECT_SAMPLE_FACTORS - NASH020 Diagnosis:Normal GENDER=M; BMI=29; ETHNICITY=White; AGE=33; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=91 SUBJECT_SAMPLE_FACTORS - NASH024 Diagnosis:Normal GENDER=F; BMI=35; ETHNICITY=Black; AGE=43; AST=20; ALT=13; ALKP=71; TBIL=0.9; GLUCOSE=95 SUBJECT_SAMPLE_FACTORS - NASH034 Diagnosis:Normal GENDER=F; BMI=51.5; ETHNICITY=White; AGE=49; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=172 SUBJECT_SAMPLE_FACTORS - NASH036 Diagnosis:Normal GENDER=F; BMI=39; ETHNICITY=Black; AGE=45; AST=21; ALT=23; ALKP=111; TBIL=0.2; GLUCOSE=99 SUBJECT_SAMPLE_FACTORS - NASH041 Diagnosis:Normal GENDER=F; BMI=63; ETHNICITY=Black; AGE=27; AST=21; ALT=19; ALKP=86; TBIL=0.3; GLUCOSE=106 SUBJECT_SAMPLE_FACTORS - NASH042 Diagnosis:Normal GENDER=F; BMI=42; ETHNICITY=White; AGE=58; AST=18; ALT=16; ALKP=98; TBIL=0.4; GLUCOSE=105 SUBJECT_SAMPLE_FACTORS - NASH043 Diagnosis:Normal GENDER=F; BMI=52; ETHNICITY=White; AGE=39; AST=13; ALT=12; ALKP=76; TBIL=0.3; GLUCOSE=104 SUBJECT_SAMPLE_FACTORS - NASH045 Diagnosis:Normal GENDER=F; BMI=48; ETHNICITY=Black; AGE=43; AST=13; ALT=15; ALKP=90; TBIL=0.3; GLUCOSE=104 SUBJECT_SAMPLE_FACTORS - NASH046 Diagnosis:Normal GENDER=F; BMI=40; ETHNICITY=White; AGE=36; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=104 SUBJECT_SAMPLE_FACTORS - NASH050 Diagnosis:Normal GENDER=F; BMI=52.5; ETHNICITY=Black; AGE=38; AST=15; ALT=12; ALKP=75; TBIL=0.1; GLUCOSE=96 SUBJECT_SAMPLE_FACTORS - NASH051 Diagnosis:Normal GENDER=F; BMI=50.6; ETHNICITY=White; AGE=29; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=99 SUBJECT_SAMPLE_FACTORS - NASH053 Diagnosis:Normal GENDER=F; BMI=25.1; ETHNICITY=White; AGE=56; AST=26; ALT=14; ALKP=180; TBIL=0.8; GLUCOSE=140 SUBJECT_SAMPLE_FACTORS - NASH054 Diagnosis:Normal GENDER=F; BMI=30.8; ETHNICITY=White; AGE=83; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=90 SUBJECT_SAMPLE_FACTORS - NASH060 Diagnosis:Normal GENDER=F; BMI=39.7; ETHNICITY=Black; AGE=36; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=115 SUBJECT_SAMPLE_FACTORS - NASH066 Diagnosis:Normal GENDER=F; BMI=40.3; ETHNICITY=Black; AGE=52; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=95 SUBJECT_SAMPLE_FACTORS - NASH067 Diagnosis:Normal GENDER=F; BMI=39.2; ETHNICITY=Black; AGE=33; AST=17; ALT=14; ALKP=73; TBIL=0.2; GLUCOSE=87 SUBJECT_SAMPLE_FACTORS - NASH070 Diagnosis:Normal GENDER=F; BMI=25.7; ETHNICITY=White; AGE=53; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH077 Diagnosis:Normal GENDER=M; BMI=33; ETHNICITY=White; AGE=67; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=193 SUBJECT_SAMPLE_FACTORS - NASH078 Diagnosis:Normal GENDER=F; BMI=45; ETHNICITY=Black; AGE=40; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=101 SUBJECT_SAMPLE_FACTORS - NASH080 Diagnosis:Normal GENDER=F; BMI=50; ETHNICITY=White; AGE=57; AST=26; ALT=28; ALKP=54; TBIL=0.3; GLUCOSE=155 SUBJECT_SAMPLE_FACTORS - NASH082 Diagnosis:Normal GENDER=M; BMI=34; ETHNICITY=White; AGE=73; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH085 Diagnosis:Normal GENDER=F; BMI=32; ETHNICITY=White; AGE=63; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH086 Diagnosis:Normal GENDER=F; BMI=38; ETHNICITY=White; AGE=35; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=99 SUBJECT_SAMPLE_FACTORS - NASH089 Diagnosis:Normal GENDER=F; BMI=52; ETHNICITY=White; AGE=49; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=- SUBJECT_SAMPLE_FACTORS - NASH091 Diagnosis:Normal GENDER=F; BMI=29; ETHNICITY=White; AGE=68; AST=14; ALT=18; ALKP=66; TBIL=0.4; GLUCOSE=107 SUBJECT_SAMPLE_FACTORS - NASH001 Diagnosis:Steatosis GENDER=M; BMI=52.3; ETHNICITY=Black; AGE=37; AST=16; ALT=19; ALKP=58; TBIL=0.4; GLUCOSE=236 SUBJECT_SAMPLE_FACTORS - NASH002 Diagnosis:Steatosis GENDER=F; BMI=34.9; ETHNICITY=White; AGE=57; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=91 SUBJECT_SAMPLE_FACTORS - NASH023 Diagnosis:Steatosis GENDER=M; BMI=34; ETHNICITY=White; AGE=56; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=97 SUBJECT_SAMPLE_FACTORS - NASH032 Diagnosis:Steatosis GENDER=F; BMI=45; ETHNICITY=White; AGE=58; AST=27; ALT=20; ALKP=83; TBIL=0.9; GLUCOSE=89 SUBJECT_SAMPLE_FACTORS - NASH033 Diagnosis:Steatosis GENDER=F; BMI=40; ETHNICITY=White; AGE=58; AST=38; ALT=29; ALKP=102; TBIL=0.5; GLUCOSE=109 SUBJECT_SAMPLE_FACTORS - NASH056 Diagnosis:Steatosis GENDER=F; BMI=45.1; ETHNICITY=White; AGE=51; AST=38; ALT=28; ALKP=45; TBIL=0.5; GLUCOSE=99 SUBJECT_SAMPLE_FACTORS - NASH058 Diagnosis:Steatosis GENDER=M; BMI=42.7; ETHNICITY=Black; AGE=53; AST=28; ALT=34; ALKP=72; TBIL=0.6; GLUCOSE=174 SUBJECT_SAMPLE_FACTORS - NASH059 Diagnosis:Steatosis GENDER=F; BMI=49.8; ETHNICITY=White; AGE=45; AST=16; ALT=16; ALKP=84; TBIL=0.3; GLUCOSE=92 SUBJECT_SAMPLE_FACTORS - NASH061 Diagnosis:Steatosis GENDER=F; BMI=56.7; ETHNICITY=White; AGE=29; AST=64; ALT=50; ALKP=68; TBIL=0.8; GLUCOSE=91 SUBJECT_SAMPLE_FACTORS - NASH062 Diagnosis:Steatosis GENDER=F; BMI=56.6; ETHNICITY=White; AGE=51; AST=15; ALT=16; ALKP=58; TBIL=0.5; GLUCOSE=90 SUBJECT_SAMPLE_FACTORS - NASH071 Diagnosis:Steatosis GENDER=F; BMI=40; ETHNICITY=White; AGE=33; AST=15; ALT=12; ALKP=59; TBIL=0.4; GLUCOSE=85 SUBJECT_SAMPLE_FACTORS - NASH073 Diagnosis:Steatosis GENDER=F; BMI=80.2; ETHNICITY=Black; AGE=34; AST=11; ALT=11; ALKP=90; TBIL=0.4; GLUCOSE=163 SUBJECT_SAMPLE_FACTORS - NASH075 Diagnosis:Steatosis GENDER=M; BMI=33; ETHNICITY=White; AGE=67; AST=22; ALT=34; ALKP=96; TBIL=0.5; GLUCOSE=191 SUBJECT_SAMPLE_FACTORS - NASH076 Diagnosis:Steatosis GENDER=F; BMI=50; ETHNICITY=White; AGE=51; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=92 SUBJECT_SAMPLE_FACTORS - NASH079 Diagnosis:Steatosis GENDER=F; BMI=43; ETHNICITY=White; AGE=37; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=85 SUBJECT_SAMPLE_FACTORS - NASH081 Diagnosis:Steatosis GENDER=F; BMI=46; ETHNICITY=White; AGE=65; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=119 SUBJECT_SAMPLE_FACTORS - NASH083 Diagnosis:Steatosis GENDER=M; BMI=39; ETHNICITY=White; AGE=41; AST=-; ALT=-; ALKP=-; TBIL=-; GLUCOSE=106 #COLLECTION CO:COLLECTION_SUMMARY Human samples were collected according to a protocol approved by Vanderbilt CO:COLLECTION_SUMMARY University Medical Center's Internal Review Board (#120829) and under informed CO:COLLECTION_SUMMARY written patients' consent prior to inclusion in this study. Sample sizes were CO:COLLECTION_SUMMARY selected to minimize the invasive procedures. Plasma samples were obtained from CO:COLLECTION_SUMMARY patients' blood collected during standard of care surgical procedures. Urine CO:COLLECTION_SUMMARY samples were collected from patients' Foley catheters placed for standard of CO:COLLECTION_SUMMARY care procedure. Liver samples were obtained from the excess tissue collected as CO:COLLECTION_SUMMARY part of the standard of care liver biopsies performed at the time of surgery CO:COLLECTION_SUMMARY that would otherwise be discarded. Subsequently, studies at University of CO:COLLECTION_SUMMARY California, San Diego were conducted under further auspices of University of CO:COLLECTION_SUMMARY California, San Diego Internal Review Board #121220. CO:SAMPLE_TYPE Liver #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Liver sample extraction. Approximately 10 mg of frozen liver was homogenized in SP:SAMPLEPREP_SUMMARY 500 µl of cold (-20°C) 70% CH3OH using a tight-fit glass homogenizer SP:SAMPLEPREP_SUMMARY (Kimble/Kontes Glass Co., Vineland, NJ) for about 1 min on ice in the presence SP:SAMPLEPREP_SUMMARY of 4 µl of internal standard mix. The suspension was vortexed for 10 s and left SP:SAMPLEPREP_SUMMARY in an ice bath for 30 min. After mixing by vortex at 4°C for 1 min and SP:SAMPLEPREP_SUMMARY centrifugation (4°C, 18,000 g, 10 min), the supernatant was collected and the SP:SAMPLEPREP_SUMMARY solvent was evaporated. The residue was dissolved in 200 µl of resuspension SP:SAMPLEPREP_SUMMARY solvent, vortexed to mix (1 min at 4°C), and centrifuged (4°C, 18,000 g, 10 SP:SAMPLEPREP_SUMMARY min) to remove any insoluble material. GPLs: GPLs from liver samples were SP:SAMPLEPREP_SUMMARY extracted and analyzed by MS essentially as described in (20, 21). Extraction SP:SAMPLEPREP_SUMMARY and analysis of plasma samples was according to previously published procedures SP:SAMPLEPREP_SUMMARY (22). Cardiolipin, coenzyme Q, and dolichol: Lipid extractions were performed SP:SAMPLEPREP_SUMMARY based on the Bligh and Dyer method with minor modifications (23-25). FAs and SP:SAMPLEPREP_SUMMARY eicosanoids: FFAs were extracted essentially as previously described after SP:SAMPLEPREP_SUMMARY supplementation with deuterated internal standards (Cayman Chemicals) (26, 27). SP:SAMPLEPREP_SUMMARY Eicosanoids were isolated via solid phase extraction, utilizing 25 deuterated SP:SAMPLEPREP_SUMMARY internal standards (28, 29). Sterols and oxysterols: Sterols and oxysterols were SP:SAMPLEPREP_SUMMARY extracted using previously described methods (30). Neutral lipids: Cholesteryl SP:SAMPLEPREP_SUMMARY esters (CEs), TAGs, and DAGs were extracted from weighed liver tissue (0.5-1 mg) SP:SAMPLEPREP_SUMMARY suspended in 0.5 ml PBS that had been homogenized by sonication. Extractions of SP:SAMPLEPREP_SUMMARY plasma (0.05 ml diluted to 0.1 ml with PBS), urine (1 ml), and tissue sonicates SP:SAMPLEPREP_SUMMARY were carried out using 1 ml hexane:methyl t-butyl ether (1:1, v/v), essentially SP:SAMPLEPREP_SUMMARY as previously described (31). Sphingolipids: Sphingolipids from liver, plasma, SP:SAMPLEPREP_SUMMARY and urine were extracted following previously published procedures (32, 33), SP:SAMPLEPREP_SUMMARY with the exception that methylene chloride was substituted for chloroform for SP:SAMPLEPREP_SUMMARY the single-phase extraction of sphingoid bases 20. Ivanova P. T., Milne S. B., SP:SAMPLEPREP_SUMMARY Byrne M. O., Xiang Y., Brown H. A. 2007. Glycerophospholipid identification and SP:SAMPLEPREP_SUMMARY quantitation by electrospray ionization mass spectrometry. Methods Enzymol. 432: SP:SAMPLEPREP_SUMMARY 21-57. 21. Myers D. S., Ivanova P. T., Milne S. B., Brown H. A. 2011. SP:SAMPLEPREP_SUMMARY Quantitative analysis of glycerophospholipids by LC-MS: acquisition, data SP:SAMPLEPREP_SUMMARY handling, and interpretation. Biochim. Biophys. Acta. 1811: 748-757. 22. SP:SAMPLEPREP_SUMMARY Quehenberger O., Armando A. M., Brown H. A., Milne S. B., Myers D. S., Merrill SP:SAMPLEPREP_SUMMARY A. H., Jr, Bandyopadhyay S., Jones K. N., Kelly S., Shaner R. L., et al. 2010. SP:SAMPLEPREP_SUMMARY Lipidomics reveals a remarkable diversity of lipids in human plasma. J. Lipid SP:SAMPLEPREP_SUMMARY Res. 51: 3299-3305. 23. Guan Z., Li S., Smith D., Shaw W., Raetz C. 2007. SP:SAMPLEPREP_SUMMARY Identification of N-acylphosphatidylserine molecules in eukaryotic cells. SP:SAMPLEPREP_SUMMARY Biochemistry. 46: 14500-14513. 24. Tan B. K., Bogdanov M., Zhao J., Dowhan W., SP:SAMPLEPREP_SUMMARY Raetz C. R., Guan Z. 2012. Discovery of cardiolipin synthase utilizing SP:SAMPLEPREP_SUMMARY phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. SP:SAMPLEPREP_SUMMARY Acad. Sci. USA. 109: 16504-16509. 25. Wen R., Lam B., Guan Z. 2013. Aberrant SP:SAMPLEPREP_SUMMARY dolichol chain lengths as biomarkers for retinitis pigmentosa caused by impaired SP:SAMPLEPREP_SUMMARY dolichol biosynthesis. J. Lipid Res. 54: 3516-3522. 26. Quehenberger O., SP:SAMPLEPREP_SUMMARY Armando A., Dumlao D., Stephens D. L., Dennis E. A. 2008. Lipidomics analysis of SP:SAMPLEPREP_SUMMARY essential fatty acids in macrophages. Prostaglandins Leukot. Essent. Fatty SP:SAMPLEPREP_SUMMARY Acids. 79: 123-129. 27. Quehenberger O., Armando A. M., Dennis E. A. 2011. High SP:SAMPLEPREP_SUMMARY sensitivity quantitative lipidomics analysis of fatty acids in biological SP:SAMPLEPREP_SUMMARY samples by gas chromatography-mass spectrometry. Biochim. Biophys. Acta. 1811: SP:SAMPLEPREP_SUMMARY 648-656. 28. Deems R., Buczynski M. W., Bowers-Gentry R., Harkewicz R., Dennis SP:SAMPLEPREP_SUMMARY E. A. 2007. Detection and quantitation of eicosanoids via high performance SP:SAMPLEPREP_SUMMARY liquid chromatography-electrospray ionization-mass spectrometry. Methods SP:SAMPLEPREP_SUMMARY Enzymol. 432: 59-82. 29. Dumlao D. S., Buczynski M. W., Norris P. C., Harkewicz SP:SAMPLEPREP_SUMMARY R., Dennis E. A. 2011. High-throughput lipidomic analysis of fatty acid derived SP:SAMPLEPREP_SUMMARY eicosanoids and N-acylethanolamines. Biochim. Biophys. Acta. 1811: 724-736. 30. SP:SAMPLEPREP_SUMMARY McDonald J. G., Smith D. D., Stiles A. R., Russell D. W. 2012. A comprehensive SP:SAMPLEPREP_SUMMARY method for extraction and quantitative analysis of sterols and secosteroids from SP:SAMPLEPREP_SUMMARY human plasma. J. Lipid Res. 53: 1399-1409. 31. Hutchins P. M., Barkley R. M., SP:SAMPLEPREP_SUMMARY Murphy R. C. 2008. Separation of cellular nonpolar neutral lipids by SP:SAMPLEPREP_SUMMARY normal-phase chromatography and analysis by electrospray ionization mass SP:SAMPLEPREP_SUMMARY spectrometry. J. Lipid Res. 49: 804-813. 32. Shaner R. L., Allegood J. C., Park SP:SAMPLEPREP_SUMMARY H., Wang E., Kelly S., Haynes C. A., Sullards M. C., Merrill A. H., Jr 2009. SP:SAMPLEPREP_SUMMARY Quantitative analysis of sphingolipids for lipidomics using triple quadrupole SP:SAMPLEPREP_SUMMARY and quadrupole linear ion trap mass spectrometers. J. Lipid Res. 50: 1692-1707. SP:SAMPLEPREP_SUMMARY 33. Sullards M. C., Liu Y., Chen Y., Merrill A. H., Jr 2011. Analysis of SP:SAMPLEPREP_SUMMARY mammalian sphingolipids by liquid chromatography tandem mass spectrometry SP:SAMPLEPREP_SUMMARY (LC-MS/MS) and tissue imaging mass spectrometry (TIMS). Biochim. Biophys. Acta. SP:SAMPLEPREP_SUMMARY 1811: 838-853. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Sterols and oxysterols were measured using methods previously described (30). CH:CHROMATOGRAPHY_SUMMARY Plasma total cholesterol was measured using a Vitros 250 chemistry system CH:CHROMATOGRAPHY_SUMMARY (Ortho-Clinical Diagnostics, Rochester, NY). Plasma free cholesterol and liver CH:CHROMATOGRAPHY_SUMMARY free and total cholesterol were measured using methods adapted from (30). 30. CH:CHROMATOGRAPHY_SUMMARY McDonald J. G., Smith D. D., Stiles A. R., Russell D. W. 2012. A comprehensive CH:CHROMATOGRAPHY_SUMMARY method for extraction and quantitative analysis of sterols and secosteroids from CH:CHROMATOGRAPHY_SUMMARY human plasma. J. Lipid Res. 53: 1399-1409. CH:INSTRUMENT_NAME Multiple CH:COLUMN_NAME Multiple CH:CHROMATOGRAPHY_TYPE Core J #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME - MS:INSTRUMENT_TYPE - MS:MS_TYPE Other MS:ION_MODE UNSPECIFIED #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples NASH001 NASH002 NASH003 NASH004 NASH005 NASH006 NASH007 NASH009 NASH010 NASH011 NASH012 NASH013 NASH014 NASH015 NASH016 NASH017 NASH018 NASH019 NASH020 NASH021 NASH022 NASH023 NASH024 NASH026 NASH027 NASH028 NASH029 NASH030 NASH031 NASH032 NASH033 NASH034 NASH035 NASH036 NASH037 NASH038 NASH039 NASH040 NASH041 NASH042 NASH043 NASH044 NASH045 NASH046 NASH047 NASH048 NASH049 NASH050 NASH051 NASH052 NASH053 NASH054 NASH055 NASH056 NASH057 NASH058 NASH059 NASH060 NASH061 NASH062 NASH064 NASH065 NASH066 NASH067 NASH068 NASH069 NASH070 NASH071 NASH072 NASH073 NASH074 NASH075 NASH076 NASH077 NASH078 NASH079 NASH080 NASH081 NASH082 NASH083 NASH084 NASH085 NASH086 NASH087 NASH088 NASH089 NASH090 NASH091 Factors Diagnosis:Steatosis Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:NASH Diagnosis:Normal Diagnosis:NASH Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:NASH Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:NASH Diagnosis:NASH Diagnosis:Normal Diagnosis:NASH Diagnosis:Cirrhosis Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:NASH Diagnosis:NASH Diagnosis:Steatosis Diagnosis:Steatosis Diagnosis:Normal Diagnosis:NASH Diagnosis:Normal Diagnosis:NASH Diagnosis:NASH Diagnosis:NASH Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Normal Diagnosis:NASH Diagnosis:Normal Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Steatosis Diagnosis:NASH Diagnosis:Steatosis Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Steatosis Diagnosis:Steatosis Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Cirrhosis Diagnosis:Cirrhosis Diagnosis:Normal Diagnosis:Steatosis Diagnosis:NASH Diagnosis:Steatosis Diagnosis:NASH Diagnosis:Steatosis Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Normal Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Steatosis Diagnosis:Normal Diagnosis:Steatosis Diagnosis:NASH Diagnosis:Normal Diagnosis:Normal Diagnosis:NASH Diagnosis:NASH Diagnosis:Normal Diagnosis:NASH Diagnosis:Normal 7alpha,25-dihydroxycholesterol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 7alpha,27-dihydroxy cholesterol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0.000251 ND ND ND 0.000490 0.000251 ND 0.000179 0.000287 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 7alpha,24S-dihydroxycholesterol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 5alpha,6alpha-24S,25-diepoxy-cholesterol 0.001141 0.000383 0.000119 0.000136 0.000908 0.000088 0.000665 0.000401 0.000128 0.000640 0.000828 0.000527 0.000414 0.000201 0.000314 0.000502 0.000515 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 25-hydroxy Vitamin D3 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 25-hydroxy Vitamin D2 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 22R-hydroxy-cholesterol 0.000448 0.000952 0.000254 0.000699 0.000332 0.000581 0.000282 0.000142 0.000327 0.000510 0.000323 0.000224 0.000733 0.000249 0.000099 0.000236 0.000249 0.000596 0.000410 0.000373 0.000447 0.000124 0.000472 0.000186 0.000137 0.000062 0.000137 0.000199 0.000062 0.000025 0.000037 0.000012 0.000012 0.000062 0.000050 0.000050 0.000025 0.000075 0.000075 0.000137 0.000199 0.000174 0.000671 0.000249 0.000099 0.000186 0.000087 0.000298 0.000137 0.000162 0.000261 0.000634 0.000261 0.000112 0.000186 0.000162 0.000609 0.000472 0.000174 0.000298 0.000211 0.000037 0.000311 0.000721 0.000075 0.000149 0.000609 0.000336 0.000075 0.000186 0.000137 0.000211 0.000211 0.000249 0.000373 0.000186 0.000186 0.000186 0.000336 0.000199 0.000062 0.000522 0.000112 0.000311 0.000584 0.000497 0.000149 0.000124 25-hydroxy-cholesterol 0.000151 0.000128 0.000058 0.000091 0.000106 0.000039 0.000064 0.000128 0.000061 0.000072 0.000166 0.000058 0.000091 0.000106 0.000039 0.000064 0.000083 0.010526 0.000012 0.000075 0.000124 0.000273 0.000087 0.000062 0.000112 0.000087 0.000050 0.000087 0.000062 0.000025 0.000037 0.000012 0.000012 0.000062 0.000050 0.000050 0.000025 0.000087 0.000075 0.000037 0.000012 0.000025 0.000050 ND 0.000050 0.000037 0.000124 0.000037 ND 0.000037 0.000050 0.000037 ND 0.000037 0.000037 0.000062 0.000050 0.000037 0.000037 0.000050 0.000037 0.000075 ND 0.000037 0.000062 0.000025 0.000012 0.000050 0.000025 0.000050 0.000025 0.000062 0.000050 0.000062 0.000050 0.000062 0.000037 0.000050 0.000149 0.000037 0.000062 0.000050 0.000025 0.000075 0.000025 0.000025 0.000050 0.000050 24S-hydroxy-cholesterol 0.000562 0.001473 0.000243 0.000339 0.000900 0.000117 0.000635 0.000593 0.000443 0.000186 0.000360 0.000385 0.000137 0.000534 0.000174 0.000373 0.000510 0.001330 0.002050 0.001143 0.002088 0.001031 0.000833 0.001193 0.000932 0.000746 0.000895 0.001305 0.000211 0.000336 0.000360 0.000385 0.000336 0.000261 0.000360 0.000311 0.000236 0.000373 0.000224 0.000099 0.000112 0.000336 0.000311 0.000360 0.000497 0.000485 0.000273 0.000460 0.000186 0.000186 0.000186 0.000323 0.000249 0.000360 0.000124 0.000447 0.000746 0.000460 0.000311 0.000348 0.000572 0.000348 0.000174 0.000373 0.000298 0.000199 0.000137 0.000323 0.000162 0.000249 0.000485 0.000186 0.000497 0.000236 0.000497 0.000249 0.000186 0.000522 0.000584 0.000522 0.001118 0.001031 0.000385 0.000572 0.000373 0.000298 0.000770 0.000572 27-hydroxy-cholesterol 0.001615 0.002137 0.000928 0.001120 0.000708 0.000843 0.000608 0.000523 0.000920 0.000758 0.001181 0.000360 0.000472 0.001168 0.000286 0.000360 0.001181 0.000758 0.001255 0.001379 0.001653 0.001939 0.000808 0.000659 0.004449 0.000348 0.000460 0.001516 0.000820 0.000895 0.000833 0.000621 0.001665 0.000671 0.001131 0.001429 0.001255 0.000808 0.000410 0.001280 0.001181 0.001355 0.000895 0.001255 0.001094 0.000596 0.000683 0.001081 0.000895 0.000609 0.000944 0.000609 0.000510 0.001765 0.001205 0.001740 0.002137 0.001305 0.001504 0.000795 0.000646 0.000932 0.001118 0.001205 0.001143 0.000646 0.000708 0.001280 0.001168 0.000982 0.001466 0.000957 0.001603 0.000969 0.001031 0.001392 0.001243 0.001205 0.000447 0.001516 0.001864 0.000522 0.000659 0.000920 0.001131 0.000783 0.001901 0.000298 24-oxocholesterol 0.001204 0.000548 0.000484 0.000393 0.000663 0.000415 0.000291 0.000412 0.000297 0.001624 0.000924 0.000662 0.000262 0.000437 0.000250 0.000275 0.000337 0.001973 0.001873 0.002973 0.002123 0.001961 0.001786 0.003197 0.002036 0.001586 0.001561 0.001299 0.000525 0.000412 0.000462 0.000500 0.000425 0.000412 0.000325 0.000387 0.000337 0.001923 0.000350 0.005383 0.002760 0.003047 0.002798 0.003222 0.001973 0.000600 0.003260 0.002973 0.003210 0.003210 0.003709 0.000949 0.002061 0.005446 0.002498 0.003485 0.003147 0.002685 0.003522 0.001561 0.001961 0.004921 0.002973 0.002585 0.007681 0.003522 0.002348 0.003722 0.002910 0.003922 0.002985 0.003535 0.003759 0.002635 0.000774 0.002198 0.003610 0.002311 0.000525 0.000512 0.001049 0.000787 0.000537 0.000550 0.000637 0.000500 0.000437 0.001099 24,25-epoxy-cholesterol 0.000109 0.000028 0.000010 0.000024 ND 0.000029 0.000023 0.000071 0.000107 0.000025 0.000012 0.000025 0.000012 0.000025 0.000012 0.000012 0.000025 0.000087 0.000125 0.000125 0.000087 0.000112 0.000112 0.000112 0.000075 0.000100 0.000050 0.000075 0.000012 0.000012 0.000012 0.000012 0.000012 ND 0.000037 0.000012 ND 0.000025 0.000112 0.000012 0.000012 ND 0.000012 0.000012 0.000037 0.000025 0.000037 0.000037 0.000025 0.000012 0.000025 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000025 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000012 0.000025 0.000025 0.000025 0.000025 0.000025 0.000037 0.000012 0.000050 0.000025 0.000012 0.000025 0.000337 0.000287 0.000012 0.000075 0.000037 0.000012 0.000025 0.000137 3beta,5alpha,6beta-trihydroxycholestanol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 20-hydroxycholesterol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 7alpha-hydroxy-cholesterol 0.006828 0.005447 0.001579 0.001249 0.002013 0.001443 0.001215 0.003113 0.003102 0.001814 0.001789 0.001094 0.000994 0.002684 0.001081 0.001740 0.002187 0.002026 0.001690 0.003840 0.002846 0.005033 0.001777 0.002274 0.001752 0.001491 0.001143 0.001988 0.003181 0.003741 0.002697 0.003281 0.004971 0.002523 0.004697 0.004126 0.003169 0.002299 0.002635 0.001628 0.001255 0.002100 0.001963 0.001504 0.001901 0.002224 0.002759 0.002659 0.001839 0.001665 0.002895 0.001504 0.002212 0.002709 0.002548 0.003156 0.006064 0.002150 0.001616 0.001777 0.001529 0.002001 0.001392 0.002622 0.002920 0.001268 0.001417 0.001814 0.002535 0.002249 0.002746 0.001603 0.002311 0.002137 0.004673 0.003219 0.001479 0.002200 0.007568 0.002846 0.003318 0.003206 0.003778 0.002895 0.002423 0.001901 0.003629 0.003368 7-oxo-cholesterol 0.009942 0.007447 0.002540 0.002540 0.007125 0.002968 0.002978 0.012889 0.003247 0.009367 0.011903 0.003172 0.001399 0.008331 0.003560 0.004484 0.010179 0.007194 0.005296 0.006420 0.006794 0.003422 0.004784 0.007781 0.006107 0.004371 0.003510 0.003247 0.007107 0.005520 0.004309 0.018023 0.006345 0.013127 0.008043 0.006620 0.008605 0.003734 0.009005 0.005658 0.001162 0.002123 0.002061 0.009442 0.007794 0.010616 0.005683 0.009979 0.008193 0.006132 0.015113 0.001624 0.004684 0.006794 0.005770 0.006445 0.005895 0.003834 0.001848 0.004759 0.002435 0.007007 0.007831 0.017935 0.012390 0.002036 0.006157 0.015113 0.002111 0.009205 0.005820 0.008156 0.003734 0.006482 0.018472 0.007431 0.001112 0.008955 0.039867 0.004796 0.004471 0.006045 0.006757 0.004908 0.003385 0.003684 0.007044 0.004596 19-hydroxycholesterol ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 6alpha-hydroxy-cholestanol 0.000132 ND ND 0.000228 0.000232 0.000163 ND 0.000253 0.000125 0.000050 0.000037 0.000037 0.000050 0.000025 0.000062 0.000062 0.000050 ND ND ND ND ND ND ND ND ND ND ND 0.000099 0.000149 0.000062 0.000050 0.000062 0.000062 0.000087 0.000062 0.000087 ND 0.000112 0.000050 ND ND 0.000075 ND 0.000099 0.000099 0.000099 0.000050 ND 0.000075 0.000124 ND 0.000062 ND ND 0.000062 0.000149 0.000087 ND ND 0.000137 ND 0.000062 0.000112 0.000099 0.000087 0.000062 ND 0.000062 0.000087 ND 0.000062 ND 0.000037 ND ND ND ND 0.000137 0.000062 0.000012 0.000087 0.000037 0.000050 0.000025 0.000050 0.000037 0.000075 5alpha-hydroxycholesterol 0.001282 0.001252 0.000484 0.000331 0.000892 0.000401 ND 0.001772 0.000466 0.000696 0.000708 0.000460 0.000336 0.000323 0.000249 0.000485 0.000634 0.000659 0.000360 0.001044 0.000671 0.000485 0.000286 0.000721 0.000447 0.000460 0.000398 0.000137 0.000795 0.000435 0.000770 0.000224 0.000746 0.000721 0.000572 0.000211 0.000087 0.000969 0.000795 0.000435 0.000360 0.000708 0.000795 0.000547 0.000671 0.000746 0.000311 0.000758 0.000273 0.000559 0.000870 0.000472 0.000708 0.001156 0.000472 0.001292 0.001876 0.000708 0.000460 0.000522 0.000646 0.000721 0.000485 0.000360 0.001131 0.000410 0.000534 0.000360 0.000559 0.000783 0.000857 0.000497 0.000447 0.000634 0.001243 0.000833 0.000348 0.000659 0.000410 0.000472 0.000547 0.000435 0.000423 0.000460 0.000348 0.000472 0.000447 0.000311 5,6beta-epoxy-cholesterol 0.008286 0.005726 0.002610 0.001717 0.005760 0.002595 0.002454 0.017087 0.001496 0.002933 0.002510 0.002100 0.001143 0.001429 0.001193 0.002635 0.001740 0.003604 0.002361 0.002510 0.004561 0.001765 0.002137 0.004760 0.004387 0.003430 0.002423 0.002597 0.005008 0.003691 0.005232 0.008401 0.004946 0.003629 0.005828 0.004735 0.005816 0.005468 0.004163 0.002137 0.004722 0.003069 0.003554 0.002398 0.004163 0.006549 0.003057 0.003989 0.002187 0.003492 0.005592 0.002585 0.004523 0.004325 0.002374 0.007953 0.008326 0.004188 0.001951 0.002311 0.004536 0.002858 0.002448 0.002026 0.010426 0.002113 0.002187 0.002597 0.002659 0.002485 0.002908 0.003156 0.002995 0.003094 0.007295 0.003952 0.002038 0.002958 0.007245 0.005021 0.005095 0.004635 0.003579 0.004101 0.002771 0.004635 0.004598 0.002647 5,6alpha-epoxy-cholesterol 0.004680 0.002039 0.001110 0.001077 0.002398 0.001414 0.001177 0.005058 0.001088 0.000870 0.000845 0.000596 0.000472 0.000596 0.000547 0.000870 0.000596 0.001292 0.000920 0.001355 0.001715 0.000882 0.001044 0.001616 0.001379 0.001280 0.001143 0.001094 0.002461 0.002001 0.002386 0.002436 0.002200 0.001827 0.003045 0.002485 0.003132 0.002038 0.001702 0.000808 0.000895 0.001205 0.001255 0.001156 0.001529 0.001616 0.001268 0.001591 0.000833 0.001031 0.001777 0.001143 0.001379 0.001740 0.000783 0.002386 0.003368 0.001603 0.001019 0.000932 0.001330 0.001268 0.001181 0.001205 0.002560 0.001019 0.001168 0.000845 0.001019 0.001268 0.001218 0.001156 0.001156 0.001292 0.004673 0.001392 0.000895 0.000969 0.002100 0.001491 0.001479 0.001777 0.001143 0.001429 0.000969 0.001292 0.001292 0.001031 4beta-hydroxy-cholesterol 0.000553 0.001102 0.000492 0.002575 0.000376 0.000978 0.000611 0.000349 0.000372 0.000920 0.000820 0.000795 0.000944 0.000621 0.000510 0.000398 0.000497 0.000273 0.000398 0.000783 0.001143 0.000311 0.001205 0.001230 0.000435 0.000249 0.000721 0.000534 0.000596 0.001789 0.000708 0.000584 0.000485 0.005518 0.000808 0.000336 0.001094 0.000646 0.000398 0.000360 0.000634 0.001081 0.000920 0.000373 0.000708 0.001578 0.000360 0.000683 0.000385 0.001094 0.001479 0.000435 0.000485 0.000621 0.000373 0.000485 0.004163 0.001118 0.000447 0.000323 0.000659 0.000423 0.000857 0.001094 0.001069 0.000398 0.001218 0.000559 0.000522 0.000795 0.000559 0.000410 0.000559 0.000510 0.001479 0.000547 0.000584 0.000472 0.000783 0.000547 0.000249 0.000733 0.001591 0.000659 0.000423 0.000621 0.000808 0.000683 Zymosterol ND ND ND ND ND ND ND 0.000455 0.001340 ND ND ND ND ND ND ND ND ND 0.000195 ND 0.000065 ND 0.000039 0.000078 ND 0.000117 0.000911 0.000299 ND ND 0.000052 ND 0.000117 ND 0.000104 0.000169 ND ND ND 0.000104 0.000260 ND ND 0.000221 0.000065 ND ND ND ND ND ND ND ND ND ND ND ND ND 0.000351 ND ND ND ND ND 0.000247 ND ND ND 0.000208 0.001249 ND 0.000286 0.000156 0.000104 ND 0.000494 0.000091 ND 0.000143 ND 0.000455 ND 0.000247 ND 0.000260 ND ND 0.000208 Desmosterol 0.008271 0.008936 0.003434 0.005022 0.013087 0.001951 0.007116 0.015650 0.002966 0.002888 0.004007 0.006635 0.003057 0.003981 0.001288 0.001626 0.005412 0.003226 0.006375 0.001600 0.009653 0.012138 0.003838 0.011318 0.005490 0.009770 0.002199 0.007584 0.005061 0.001782 0.004618 0.004957 0.006934 0.002134 0.005750 0.008326 0.004280 0.006166 0.002394 0.003421 0.004098 0.006583 0.005191 0.002875 0.006283 0.006388 0.009067 0.005945 0.004371 0.004423 0.001483 0.003330 0.002563 0.008443 0.004059 0.006270 0.013660 0.004657 0.016821 0.002576 0.008235 0.004800 0.002810 0.003812 0.004566 0.006283 0.003018 0.004319 0.011084 0.030975 0.007168 0.002134 0.007077 0.004254 0.007936 0.004826 0.002550 0.004293 0.001847 0.004410 0.011110 0.005451 0.003083 0.004254 0.012684 0.006908 0.008651 0.001860 14-demethyl-14-dehydrolanosterol 0.000666 0.000656 0.000889 0.000231 0.000097 0.000268 0.000158 0.000195 0.002009 0.000292 0.001120 0.000268 0.000280 0.000609 0.000097 0.000158 0.000743 0.000183 0.000536 0.000804 0.000061 0.001461 0.000195 0.000134 0.000049 0.000170 0.000073 0.001303 0.000377 0.000645 0.000414 0.000450 0.000852 0.000061 0.000718 0.001644 0.000207 0.000158 0.000195 0.000256 0.000524 0.000767 0.000268 0.000731 0.000158 0.000231 0.000110 0.000499 0.000207 0.000085 0.000244 0.000134 0.000219 0.000280 0.000231 0.000353 0.000877 0.000110 0.000621 0.000877 0.000061 0.000256 0.000134 0.000183 0.000085 0.000110 0.000158 0.000329 0.000487 0.000268 0.000377 0.000341 0.000426 0.000097 0.000560 0.001157 0.000195 0.000889 0.000146 0.000146 0.000852 0.000390 0.000511 0.000304 0.001583 0.000609 0.000511 0.000268 8-Dehydrochol 0.004629 0.009337 0.004592 0.004683 0.004423 0.001717 0.004696 0.004696 0.014544 0.003539 0.004631 0.002277 0.002524 0.020490 0.017133 0.004124 0.007506 0.003786 0.004592 0.094005 0.006531 0.009835 0.001717 0.003838 0.002225 0.001990 0.008846 0.011058 0.003343 0.035633 0.003890 0.005945 0.010407 0.001938 0.009874 0.009913 0.005581 0.003890 0.001639 0.007936 0.004670 0.002953 0.004423 0.006101 0.002615 0.002680 0.002147 0.002003 0.002758 0.002407 0.005048 0.005672 0.010720 0.005464 0.022519 0.008872 0.019033 0.004202 0.003669 0.012671 0.002940 0.003591 0.002003 0.001821 0.002563 0.001847 0.002602 0.004059 0.004150 0.000351 0.007246 0.006817 0.003825 0.003812 0.006791 0.009041 0.005958 0.042606 0.006427 0.005620 0.007090 0.003799 0.024314 0.003278 0.002719 0.005165 0.007259 0.038456 7-Dehydrochol 0.007011 0.007756 0.005880 0.005204 0.003395 0.002979 0.003864 0.005087 0.042397 0.004696 0.008001 0.004592 0.005607 0.012671 0.013465 0.009835 0.007897 0.004319 0.005867 0.074595 0.004735 0.005243 0.004852 0.004332 0.003395 0.004384 0.004566 0.008937 0.003460 0.047770 0.003994 0.005542 0.007689 0.003369 0.006518 0.007350 0.004944 0.007168 0.004332 0.006049 0.006622 0.010707 0.005971 0.007168 0.004683 0.004957 0.003942 0.008170 0.004475 0.007376 0.012697 0.006856 0.010147 0.007272 0.005802 0.016236 0.022298 0.010225 0.006101 0.027411 0.006153 0.006179 0.005503 0.005607 0.004931 0.005074 0.005334 0.006570 0.005568 0.005724 0.008118 0.012294 0.006947 0.005607 0.010538 0.014466 0.006726 0.051777 0.006713 0.006440 0.005633 0.003682 0.018460 0.005113 0.006596 0.004371 0.006531 0.044102 Cholestenone 0.000246 0.000600 0.000377 0.000208 0.000429 0.000273 0.000312 0.000260 0.000312 0.000768 0.000585 0.000312 0.000377 0.000559 0.000195 0.000364 0.000689 0.000169 0.000273 0.000286 0.000377 0.000703 0.000299 0.000273 0.000169 0.000221 0.000390 0.000624 0.000286 0.000507 0.000312 0.000247 0.000325 0.000143 0.000377 0.000364 0.000507 0.000481 0.000377 0.000169 0.000572 0.000364 0.000716 0.000169 0.000937 0.000885 0.000156 0.000742 0.000429 0.000676 0.001119 0.000742 0.000273 0.000794 0.000221 0.000950 0.003565 0.001093 0.000585 0.000598 0.000611 0.000507 0.000195 0.000273 0.000221 0.000364 0.000768 0.000429 0.000755 0.000377 0.000742 0.000520 0.000585 0.001093 0.000273 0.000572 0.000312 0.000260 0.000572 0.000455 0.000807 0.000364 0.000312 0.000416 0.000637 0.000546 0.000598 0.000494 14-Demethyllanosterol 0.004467 0.006370 0.004899 0.004838 0.005420 0.003904 0.004777 0.006172 0.003747 0.003468 0.003892 0.004450 0.004947 0.004802 0.004304 0.003213 0.004802 0.006390 0.004462 0.007142 0.012380 0.004547 0.007675 0.011373 0.008657 0.004692 0.009736 0.006923 0.002910 0.008063 0.003140 0.005517 0.003456 0.015750 0.003359 0.002546 0.021898 0.003407 0.003929 0.002655 0.003832 0.022116 0.003710 0.002219 0.003383 0.010149 0.005517 0.004341 0.002946 0.004826 0.041055 0.002364 0.002849 0.006851 0.011664 0.008451 0.014017 0.004511 0.004850 0.001746 0.009458 0.004802 0.005371 0.007906 0.006111 0.005978 0.003092 0.005262 0.003213 0.005565 0.003068 0.002777 0.003347 0.003153 0.005371 0.003844 0.003286 0.004426 0.003601 0.006499 0.004171 0.003031 0.001928 0.003262 0.003565 0.002765 0.002158 0.003347 Lanosterol 0.000010 0.000010 0.000016 0.000002 0.000004 0.000006 0.000004 0.000002 0.000027 0.001794 0.005136 0.005160 0.002463 0.001478 0.000786 0.000739 0.004843 0.001771 0.006004 0.005863 0.002744 0.015373 0.002779 0.001747 0.000879 0.000915 0.002592 0.008631 0.008291 0.006942 0.003131 0.007059 0.013755 0.003389 0.006836 0.009991 0.005887 0.002908 0.003236 0.003166 0.003577 0.009838 0.002146 0.004913 0.001958 0.001266 0.001055 0.005300 0.003131 0.000199 0.002885 0.001736 0.002146 0.003999 0.001653 0.003799 0.007552 0.001900 0.005980 0.006051 0.000457 0.002521 0.000797 0.002240 0.001302 0.001079 0.001829 0.003448 0.006438 0.005207 0.004468 0.001278 0.005910 0.002943 0.007493 0.006942 0.002334 0.006426 0.001231 0.000985 0.007212 0.003881 0.008244 0.005863 0.012582 0.008220 0.004304 0.001736 Cholestanol 0.036346 0.022560 0.018024 0.034902 0.046193 0.027937 0.035379 0.047081 0.015488 0.021616 0.014162 0.028735 0.019723 0.015771 0.037645 0.015784 0.020135 0.013904 0.021423 0.016724 0.072302 0.022878 0.023946 0.042974 0.025350 0.026959 0.075083 0.023290 0.019749 0.056068 0.016775 0.036434 0.039267 0.034220 0.036537 0.016067 0.029302 0.019105 0.030396 0.010299 0.015076 0.016569 0.015514 0.009154 0.032508 0.046708 0.017445 0.013132 0.012758 0.028761 0.026727 0.013441 0.013582 0.021899 0.014947 0.021487 0.047197 0.027525 0.014097 0.011059 0.050158 0.019453 0.019427 0.013982 0.026920 0.040593 0.017084 0.012707 0.023406 0.020959 0.019814 0.012334 0.015089 0.015372 0.016041 0.012977 0.010235 0.014805 0.017187 0.019492 0.016801 0.012733 0.010093 0.009514 0.012527 0.016659 0.019672 0.011317 Campesterol 0.072275 0.037081 0.033132 0.043834 0.057110 0.039426 0.042948 0.053613 0.040400 0.039289 0.053226 0.043322 0.054038 0.051815 0.042173 0.039189 0.054937 0.041287 0.055736 0.075018 0.119127 0.047781 0.050416 0.071971 0.062642 0.039239 0.062979 0.064328 0.040350 0.083648 0.043897 0.047544 0.039164 0.049105 0.057384 0.057397 0.041149 0.039301 0.040887 0.029598 0.034031 0.035142 0.035167 0.029610 0.029273 0.086470 0.038989 0.044359 0.030147 0.040712 0.035068 0.033369 0.033994 0.046345 0.044521 0.054175 0.094538 0.057971 0.032994 0.031383 0.068287 0.040038 0.047556 0.037565 0.038227 0.042661 0.031833 0.033844 0.031009 0.033594 0.055087 0.029310 0.034730 0.030347 0.056260 0.049816 0.028599 0.045845 0.033606 0.059620 0.036941 0.041099 0.042760 0.049929 0.047069 0.038527 0.039651 0.041462 Stigmasterol 0.002629 0.001968 0.002073 0.002886 0.002207 0.001795 0.001770 0.002813 0.001516 0.003771 0.002183 0.002304 0.002219 0.003262 0.003771 0.002886 0.003577 ND ND ND ND ND ND ND ND ND ND ND 0.001297 0.005505 0.001297 0.001928 0.001601 0.006390 0.002849 0.001431 0.009264 0.002389 0.002013 0.003807 0.003444 0.012974 0.003322 0.002607 0.002037 0.004705 0.003589 0.004147 0.003274 0.003274 0.023995 0.002049 0.002413 0.005832 0.007493 0.005772 0.009603 0.003492 0.003904 0.001564 0.005384 0.005129 0.004753 0.005299 0.006050 0.004899 0.002801 0.004656 0.003638 0.004608 0.003795 0.003541 0.003165 0.002849 0.005020 0.003856 0.003262 0.003625 0.002377 0.004050 0.002195 0.003347 0.001091 0.003201 0.003092 0.002267 0.002522 0.002728 Sitosterol 0.022684 0.015324 0.010449 0.016977 0.028536 0.008784 0.013924 0.020222 0.015517 0.017411 0.015758 0.020138 0.017194 0.017206 0.021912 0.010256 0.015094 0.023348 0.023999 0.028114 0.054164 0.019185 0.022672 0.047672 0.037658 0.021140 0.044970 0.036367 0.008965 0.053609 0.010799 0.016627 0.009544 0.054200 0.018413 0.010558 0.060269 0.018256 0.015999 0.023661 0.022672 0.085125 0.025797 0.017001 0.015191 0.043256 0.031577 0.024614 0.019764 0.024409 0.140206 0.014177 0.017230 0.042581 0.046357 0.037537 0.069234 0.028355 0.024289 0.011282 0.043980 0.033024 0.032047 0.037839 0.039106 0.034895 0.018509 0.028958 0.018931 0.028041 0.016132 0.023106 0.021948 0.016892 0.031009 0.024578 0.020983 0.024144 0.018280 0.029718 0.016723 0.019957 0.011077 0.019197 0.021429 0.016567 0.016518 0.019197 24-dihydro Lanosterol ND ND ND ND ND ND ND ND ND 0.000584 0.000945 0.001085 0.000712 0.000630 0.000642 0.000432 0.000957 0.001155 0.002206 0.001599 0.002603 0.002148 0.001424 0.001249 0.000910 0.000992 0.001389 0.001482 0.001074 0.003653 0.000945 0.001284 0.001482 0.001611 0.001331 0.001272 0.001552 0.001377 0.000910 0.001167 0.000957 0.001692 0.000992 0.001062 0.000747 0.000934 0.000712 0.001284 0.000922 0.000817 0.001342 0.000840 0.000595 0.001716 0.001202 0.001027 0.003373 0.000817 0.000957 0.000817 0.001284 0.001144 0.000689 0.000969 0.001167 0.000794 0.000934 0.001377 0.001727 0.000934 0.002007 0.000782 0.001109 0.000992 0.001471 0.001389 0.000782 0.001366 0.000922 0.000887 0.001307 0.000817 0.001681 0.001039 0.001342 0.001039 0.000969 0.000782 Stigmastanol 0.005106 0.001515 0.000072 0.002726 0.001453 0.001069 0.001033 0.001237 0.001189 0.001285 0.000901 0.000672 0.000865 0.000805 0.000793 0.000564 0.000504 0.000829 0.000648 0.001297 0.000961 0.000276 0.001177 0.000877 0.000853 0.001129 0.001345 0.000336 0.000588 0.001429 0.001273 0.003518 0.000913 0.001897 0.002161 0.004227 0.001681 0.000973 0.000925 0.000672 0.000901 0.001789 0.001609 0.000805 0.000889 0.001261 0.001201 0.000985 0.001837 0.000588 0.001933 0.002474 0.000468 0.001261 0.001081 0.001417 0.002690 0.000612 0.001033 0.000384 0.000744 0.000853 0.000877 0.002029 0.001213 0.001573 0.001453 0.001669 0.000612 0.000600 0.006844 0.000660 0.000768 0.001033 0.000720 0.001069 0.000480 0.002450 0.002341 0.001165 0.000348 0.000480 0.000216 0.000180 0.001141 0.000961 0.003086 0.000780 Cholesterol-free not measured not measured 6.046812 6.673995 5.457132 4.562266 4.620458 4.449761 5.771369 5.432562 8.056382 4.435536 5.507565 8.005949 4.736842 4.558386 6.773568 5.136428 4.598474 4.401914 5.618777 4.633389 6.101125 4.363119 4.381223 3.946722 5.272210 8.313720 4.817018 9.582310 6.180008 4.699340 10.880641 6.288633 6.959783 4.776930 4.013966 5.925255 5.991207 3.721712 4.224751 8.715893 5.644640 4.624337 4.740722 5.299366 5.710591 7.861115 5.017458 4.695461 4.372171 4.687702 3.318246 7.817147 4.846761 8.039571 25.194620 6.794258 4.845467 4.775637 6.163197 6.610630 9.323678 6.583473 5.106686 4.797621 6.150265 5.263158 6.001552 4.751067 8.311134 5.834734 6.114057 7.086512 10.285788 5.495927 4.352774 6.705030 3.884650 5.320057 5.176516 5.635588 4.965731 3.726885 4.718738 3.826458 8.723652 5.132549 Cholesterol-total not measured not measured 13.267813 11.884133 10.332342 7.513255 11.741885 11.366869 8.534851 9.879736 16.203285 9.207293 7.371007 11.043579 7.784818 8.909867 10.694426 8.987456 12.116902 10.616837 9.802147 9.970257 17.211949 11.147032 9.271951 10.797879 9.530583 14.379930 12.453123 14.793741 8.366740 8.935730 15.155826 10.836674 14.004914 8.030519 9.310746 8.483124 11.522048 7.228760 10.707358 12.039312 9.284883 7.474460 11.185827 11.302211 62.627699 13.901461 9.944394 7.448597 10.164231 11.315143 11.470322 12.918660 10.539247 14.418725 42.105263 13.073839 9.271951 6.750291 8.250356 7.539118 13.655761 9.892668 15.078236 12.065175 9.582310 13.875598 8.819346 10.397000 15.504979 10.862537 12.996250 15.181689 20.354326 9.685762 6.090780 18.673219 10.435795 17.522307 20.897452 16.552438 6.608043 10.953058 12.310875 9.672831 12.039312 9.698694 3-oxo,7alpha-hydroxycholesterol not measured not measured 0.000736 0.000087 0.000100 0.000237 0.000237 0.000100 0.002034 0.000187 0.000362 0.000300 0.000250 0.003657 0.000549 0.000250 0.002496 0.000786 0.001884 0.001323 0.000262 0.002558 0.000499 0.000262 0.000612 0.000137 0.000200 0.002970 0.000499 0.001585 0.000774 0.001248 0.001847 0.001123 0.002221 0.002434 0.000886 0.000936 0.001023 0.000986 0.001023 0.001710 0.001622 0.000961 0.000125 0.000175 0.000524 0.000799 0.000462 0.000125 0.000125 0.000761 0.001922 0.002022 0.003157 0.000300 xx 0.000449 0.001423 0.000661 0.000112 0.001959 0.000487 0.000262 0.001073 0.000100 0.000312 0.000686 0.000699 0.000574 0.002384 0.000612 0.000899 0.000499 0.000711 0.003682 0.000874 0.001248 0.000998 0.001373 0.003682 0.000899 0.001073 0.001123 0.001410 0.000524 0.001136 0.001810 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name 7alpha,25-dihydroxycholesterol 7alpha,27-dihydroxy cholesterol 7alpha,24S-dihydroxycholesterol 5alpha,6alpha-24S,25-diepoxy-cholesterol 25-hydroxy Vitamin D3 25-hydroxy Vitamin D2 22R-hydroxy-cholesterol 25-hydroxy-cholesterol 24S-hydroxy-cholesterol 27-hydroxy-cholesterol 24-oxocholesterol 24,25-epoxy-cholesterol 3beta,5alpha,6beta-trihydroxycholestanol 20-hydroxycholesterol 7alpha-hydroxy-cholesterol 7-oxo-cholesterol 19-hydroxycholesterol 6alpha-hydroxy-cholestanol 5alpha-hydroxycholesterol 5,6beta-epoxy-cholesterol 5,6alpha-epoxy-cholesterol 4beta-hydroxy-cholesterol Zymosterol Desmosterol 14-demethyl-14-dehydrolanosterol 8-Dehydrochol 7-Dehydrochol Cholestenone 14-Demethyllanosterol Lanosterol Cholestanol Campesterol Stigmasterol Sitosterol 24-dihydro Lanosterol Stigmastanol Cholesterol-free Cholesterol-total 3-oxo,7alpha-hydroxycholesterol METABOLITES_END #END