#METABOLOMICS WORKBENCH hatalbott2_20191118_161906 DATATRACK_ID:1852 STUDY_ID:ST001286 ANALYSIS_ID:AN002130 PROJECT_ID:PR000868 VERSION 1 CREATED_ON December 12, 2019, 5:08 pm #PROJECT PR:PROJECT_TITLE Lipid composition of isolated lipid droplets from the functional bovine corpus PR:PROJECT_TITLE luteum PR:PROJECT_TYPE lipidomics PR:PROJECT_SUMMARY Establishment and maintenance of pregnancy is dependent on progesterone PR:PROJECT_SUMMARY synthesized by the corpus luteum (CL). The CL is known for the prominent PR:PROJECT_SUMMARY presence of intracellular lipid droplets (LDs). However relatively little is PR:PROJECT_SUMMARY known about the composition and function of these luteal LDs. Our objective was PR:PROJECT_SUMMARY to identify the lipid composition of LDs from fully functional bovine CLs. PR:PROJECT_SUMMARY Luteal LDs were isolated by flotation through a discontinuous sucrose gradient, PR:PROJECT_SUMMARY lipids were then extracted using a standard Bligh and Dyer protocol, dried, and PR:PROJECT_SUMMARY sent to Avanti Polar Lipids for lipidomics analysis. The samples were provided PR:PROJECT_SUMMARY for lipidomic profiling of free sterols, cholesteryl esters, triglycerides, PR:PROJECT_SUMMARY diacylglycerols, phospholipids, and sphingolipids. Molecular species were PR:PROJECT_SUMMARY resolved by reversed-phase liquid chromatography in the presence of class and PR:PROJECT_SUMMARY sub-class specific internal standard compounds added to each sample. The PR:PROJECT_SUMMARY compounds were detected by tandem mass spectrometry (MS/MS) with scheduled PR:PROJECT_SUMMARY multiple reaction monitoring (MRM) for mass-specific fragment ions according to PR:PROJECT_SUMMARY the lipid class and molecular weight of the compound. Quantification of PR:PROJECT_SUMMARY cholesterol, cholesteryl esters, triglycerides, and diglycerides were directly PR:PROJECT_SUMMARY calculated with standards and internal standards from calibration response PR:PROJECT_SUMMARY curves. The remaining lipid species were semi-quantization using the integrated PR:PROJECT_SUMMARY area of each analyte’s MRM peak, divided by the appropriate internal standard PR:PROJECT_SUMMARY peak area, and multiplied by the standard’s known concentration. Lipid PR:PROJECT_SUMMARY concentrations were normalized to the corresponding protein concentration of PR:PROJECT_SUMMARY each sample and as a mol % relative to total lipids or within each lipid class. PR:PROJECT_SUMMARY Isolated luteal LDs were composed primarily of triglyceride (88%, mol% of lipid PR:PROJECT_SUMMARY class to total lipids). Other neutral lipids included diacylglycerol, 2.9%; and PR:PROJECT_SUMMARY cholesteryl esters, 1.5%. Polar lipids were primarily composed of PR:PROJECT_SUMMARY phosphatidylcholine (3.1%), sphingomyelin (1.5%), phosphatidylinositol (0.9%), PR:PROJECT_SUMMARY phosphatidylethanolamine (0.8%) and phosphatidylserine (0.4%). A number of other PR:PROJECT_SUMMARY minor lipids representing less than 0.32% of the total lipid pool were also PR:PROJECT_SUMMARY detected including phosphatidylglycerol, lysophospholipids, ceramides, and PR:PROJECT_SUMMARY glycosylated ceramides. Lipid composition of bovine luteal LDs are distinct from PR:PROJECT_SUMMARY LDs isolated from other tissues and in other species. PR:INSTITUTE University of Nebraska Medical Center PR:DEPARTMENT Obstetrics and Gynecology PR:LABORATORY John S. Davis PR:LAST_NAME Davis PR:FIRST_NAME John PR:ADDRESS 983255 Nebraska Medical Center Omaha, NE 68198-3255 PR:EMAIL jsdavis@unmc.edu PR:PHONE 402-599-9079 PR:FUNDING_SOURCE INBRE - P20GM103427-14, COBRE - 1P30GM110768-01 PR:CONTRIBUTORS Heather Talbott, Xiaoying Hou, Crystal Cordes #STUDY ST:STUDY_TITLE Lipid composition of isolated lipid droplets from the functional bovine corpus ST:STUDY_TITLE luteum ST:STUDY_TYPE Lipidomics ST:STUDY_SUMMARY Establishment and maintenance of pregnancy is dependent on progesterone ST:STUDY_SUMMARY synthesized by the corpus luteum (CL). The CL is known for the prominent ST:STUDY_SUMMARY presence of intracellular lipid droplets (LDs). However relatively little is ST:STUDY_SUMMARY known about the composition and function of these luteal LDs. Our objective was ST:STUDY_SUMMARY to identify the lipid composition of LDs from fully functional bovine CLs. ST:STUDY_SUMMARY Luteal LDs were isolated by flotation through a discontinuous sucrose gradient, ST:STUDY_SUMMARY lipids were then extracted using a standard Bligh and Dyer protocol, dried, and ST:STUDY_SUMMARY sent to Avanti Polar Lipids for lipidomics analysis. The samples were provided ST:STUDY_SUMMARY for lipidomic profiling of free sterols, cholesteryl esters, triglycerides, ST:STUDY_SUMMARY diacylglycerols, phospholipids, and sphingolipids. Molecular species were ST:STUDY_SUMMARY resolved by reversed-phase liquid chromatography in the presence of class and ST:STUDY_SUMMARY sub-class specific internal standard compounds added to each sample. The ST:STUDY_SUMMARY compounds were detected by tandem mass spectrometry (MS/MS) with scheduled ST:STUDY_SUMMARY multiple reaction monitoring (MRM) for mass-specific fragment ions according to ST:STUDY_SUMMARY the lipid class and molecular weight of the compound. Quantification of ST:STUDY_SUMMARY cholesterol, cholesteryl esters, triglycerides, and diglycerides were directly ST:STUDY_SUMMARY calculated with standards and internal standards from calibration response ST:STUDY_SUMMARY curves. The remaining lipid species were semi-quantization using the integrated ST:STUDY_SUMMARY area of each analyte’s MRM peak, divided by the appropriate internal standard ST:STUDY_SUMMARY peak area, and multiplied by the standard’s known concentration. Lipid ST:STUDY_SUMMARY concentrations were normalized to the corresponding protein concentration of ST:STUDY_SUMMARY each sample and as a mol % relative to total lipids or within each lipid class. ST:STUDY_SUMMARY Isolated luteal LDs were composed primarily of triglyceride (88%, mol% of lipid ST:STUDY_SUMMARY class to total lipids). Other neutral lipids included diacylglycerol, 2.9%; and ST:STUDY_SUMMARY cholesteryl esters, 1.5%. Polar lipids were primarily composed of ST:STUDY_SUMMARY phosphatidylcholine (3.1%), sphingomyelin (1.5%), phosphatidylinositol (0.9%), ST:STUDY_SUMMARY phosphatidylethanolamine (0.8%) and phosphatidylserine (0.4%). A number of other ST:STUDY_SUMMARY minor lipids representing less than 0.32% of the total lipid pool were also ST:STUDY_SUMMARY detected including phosphatidylglycerol, lysophospholipids, ceramides, and ST:STUDY_SUMMARY glycosylated ceramides. Lipid composition of bovine luteal LDs are distinct from ST:STUDY_SUMMARY LDs isolated from other tissues and in other species. ST:INSTITUTE University of Nebraska Medical Center ST:DEPARTMENT Obstetrics and Gynecology ST:LABORATORY John S. Davis ST:LAST_NAME Davis ST:FIRST_NAME John ST:ADDRESS 983255 Nebraska Medical Center Omaha, NE 68198-3255 ST:EMAIL jsdavis@unmc.edu ST:PHONE 402-559-9079 ST:NUM_GROUPS 1 ST:TOTAL_SUBJECTS 3 ST:NUM_FEMALES 3 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Bos taurus SU:TAXONOMY_ID 9913 SU:GENDER Female SU:ANIMAL_ANIMAL_SUPPLIER JBS Beef Plant 3435 Edward Babe Gomez Ave, Omaha, NE 68107 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - bovine_CL_LD_replicate1 Treatment:Control SUBJECT_SAMPLE_FACTORS - bovine_CL_LD_replicate2 Treatment:Control SUBJECT_SAMPLE_FACTORS - bovine_CL_LD_replicate3 Treatment:Control #COLLECTION CO:COLLECTION_SUMMARY Tissue (~2.5 g) was washed thoroughly in TE buffer (10 mM Tris, 1 mM EDTA, pH CO:COLLECTION_SUMMARY 7.4). Minced tissue was resuspended in 10 mL tissue homogenate buffer (60% CO:COLLECTION_SUMMARY sucrose w/v in TE buffer containing protease and phosphatase inhibitor CO:COLLECTION_SUMMARY cocktails) and homogenized with a Teflon Dounce homogenizer in a glass vessel. CO:COLLECTION_SUMMARY The post-nuclear supernatant (PNS) fraction was obtained after centrifugation at CO:COLLECTION_SUMMARY 2000 rcf for 10 min. The supernatant was loaded into a 30 mL ultracentrifuge CO:COLLECTION_SUMMARY tube and overlaid sequentially with 40%, 25%, 10%, and 0% sucrose w/v in TE CO:COLLECTION_SUMMARY buffer containing protease and phosphatase inhibitor cocktails. Samples were CO:COLLECTION_SUMMARY centrifuged at 110,000 × g (ravg) for 30 min at 4 °C with no brake in a CO:COLLECTION_SUMMARY Beckman Coulter Avanti J-20 XP ultracentrifuge using an SW 32 Ti rotor. The LDs CO:COLLECTION_SUMMARY concentrated in a yellow-ish band at the top of the gradient were harvested and CO:COLLECTION_SUMMARY concentrated by centrifugation at 2000 rcf for 10 min at 4 °C. This protocol CO:COLLECTION_SUMMARY was derived from Ding et al. 2012, and Brasaemale et al. 2016. Ding, Y., Zhang, CO:COLLECTION_SUMMARY S., Yang, L., Na, H., Zhang, P., Zhang, H., … Liu, P. (2013). Isolating lipid CO:COLLECTION_SUMMARY droplets from multiple species. Nature Protocols, 8(1), 43–51. CO:COLLECTION_SUMMARY https://doi.org/10.1038/nprot.2012.142 Brasaemle, D. L., & Wolins, N. E. (2016). CO:COLLECTION_SUMMARY Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation. CO:COLLECTION_SUMMARY Current Protocols in Cell Biology, 72, 3.15.1-3.15.13. CO:COLLECTION_SUMMARY https://doi.org/10.1002/cpcb.10 CO:SAMPLE_TYPE Ovary CO:VOLUMEORAMOUNT_COLLECTED 2.5 g of corpus luteum tissue #TREATMENT TR:TREATMENT_SUMMARY N/A #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Lipids from CL tissue LDs (~250uL) were extracted using a standard Bligh and SP:SAMPLEPREP_SUMMARY Dyer extraction protocol and then dried and sent to Avanti Polar Lipids for SP:SAMPLEPREP_SUMMARY lipidomics analysis. Extracts were received as dried residues in glass vials and SP:SAMPLEPREP_SUMMARY were immediately stored at -80 °C until analysis. Bligh, E. G., & Dyer, W. J. SP:SAMPLEPREP_SUMMARY (1959). A rapid method of total lipid extraction and purification. Canadian SP:SAMPLEPREP_SUMMARY Journal of Biochemistry and Physiology, 37(8), 911–917. SP:SAMPLEPREP_SUMMARY https://doi.org/10.1139/o59-099 SP:PROCESSING_STORAGE_CONDITIONS -80℃ SP:EXTRACTION_METHOD Bligh & Dyer, chloroform:methanol (1:2, v:v) SP:EXTRACT_STORAGE -80℃ SP:SAMPLE_RESUSPENSION 1mL of chloroform:methanol (8:2, v/v) SP:SAMPLE_DERIVATIZATION N/A SP:SUBCELLULAR_LOCATION Lipid Droplet #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Molecular species were resolved by reversed-phase liquid chromatography in the CH:CHROMATOGRAPHY_SUMMARY presence of class and sub-class specific internal standard compounds added to CH:CHROMATOGRAPHY_SUMMARY each sample. Selectivity was further enhanced by scheduling the detection of CH:CHROMATOGRAPHY_SUMMARY each compound according to its elution from the high-performance liquid CH:CHROMATOGRAPHY_SUMMARY chromatography (HPLC) column, known as scheduled MRM (sMRM). CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 1.2mm, 1.7um) CH:INTERNAL_STANDARD DG(28:0)-d5, DG(30:0)-d5, DG(32:0)-d5, DG(34:0)-d5, DG(38:0)-d5, DG(40:10)-d5, CH:INTERNAL_STANDARD DG(40:8)-d5, DG(40:4)-d5, DG(40:0)-d5, TG(44:1)-d5, TG(48:1)-d5, TG(50:0)-d5, CH:INTERNAL_STANDARD TG(51:1)-d5, TG(58:10)-d5, TG(58:7)-d5, TG(62:16)-d5, TG(60:1)-d5 #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME Avanti Polar Lipids, Inc AN:DETECTOR_TYPE AcQuRate™ Pulse Counting CEM #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS MI M+NH4 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nM MS_METABOLITE_DATA_START Samples bovine_CL_LD_replicate1 bovine_CL_LD_replicate2 bovine_CL_LD_replicate3 Factors Treatment:Control Treatment:Control Treatment:Control DG(28:3) 0.288690892 0.033618557 0.161130057 DG(28:2) 0.241678411 0.168364789 0.024239787 DG(30:4) 0.17773812 0.064020668 0.002444408 DG(30:3) 0.008542412 0.048992597 0.026251922 DG(30:2) 0.480513813 0 0 DG(32:3) 0.225935106 0.167771468 0.320563211 DG(32:2) 1.489596149 1.000470259 7.142610022 DG(32:1) 8.180266273 4.347258879 42.71847834 DG(32:0) 114.4429915 41.86611231 179.7952184 DG(34:4) 0.649277823 0.127999156 0.473780392 DG(34:3) 3.473544331 1.699301586 16.42902794 DG(34:2) 26.50518748 7.441614266 115.1173028 DG(34:1) 63.10424737 27.62987921 289.6308516 DG(34:0) 233.9014838 85.82322031 182.4792579 DG(36:5) 0.991819773 0.728871146 5.445679815 DG(36:4) 0.434060194 0.611961575 21.91767662 DG(36:3) 13.66733977 6.040622823 75.2978869 DG(36:2) 23.47172571 19.07968499 147.5007143 DG(36:1) 20.95987315 11.20552861 105.2339218 DG(36:0) 235.1825732 91.70416374 177.0248868 DG(38:6) 0.684516326 0.643408875 3.058314244 DG(38:5) 5.673904622 2.930748354 35.05927209 DG(38:4) 8.679150655 5.021118479 48.6697271 DG(38:3) 6.194183599 4.061044998 42.41149739 DG(38:2) 6.239569252 3.132026005 36.11987469 DG(38:1) 2.808178492 1.851235534 14.54726485 DG(38:0) 3.815381391 1.978376621 4.215417724 DG(40:7) 0.990336908 1.325552536 13.96338729 DG(40:6) 1.796777463 3.018785857 38.16236886 DG(40:4) 1.025174882 1.41897008 13.32190957 TG(48:4) 1.673147493 1.06742136 9.835183473 TG(48:3) 14.12897513 0.423144533 73.89881599 TG(48:2) 88.54658186 0 249.8185752 TG(48:1) 300.5769492 113.5701616 776.0884694 TG(48:0) 182.0998291 56.23878118 696.2990508 TG(50:4) 18.59236942 5.108409054 277.9494484 TG(50:3) 271.3388285 112.2858943 900.8327532 TG(50:2) 837.2233664 340.3113149 3226.904082 TG(50:1) 1335.919403 574.0693675 4681.091573 TG(50:0) 261.1356253 110.0713003 806.6900992 TG(52:6) 0 0 91.05326778 TG(52:5) 11.09784585 5.631451167 954.8032982 TG(52:4) 836.8292098 311.8625365 3150.458155 TG(52:3) 1990.741469 886.8174046 6766.08752 TG(52:2) 2773.895052 1335.720433 8969.720462 TG(52:1) 1183.415432 542.2862623 4048.363517 TG(54:7) 113.2135397 39.0696713 454.2624291 TG(54:6) 549.803904 192.4336198 2439.228926 TG(54:5) 1096.716785 363.7357541 5720.902823 TG(54:4) 796.2721331 367.3417862 5423.551681 TG(54:3) 1464.787261 703.8774665 5685.73235 TG(54:2) 977.2716847 491.6361654 4065.426509 TG(56:10) 248.8882626 93.3348648 1171.270023 TG(56:9) 724.2535767 287.5396823 3713.061669 TG(56:8) 1578.823984 690.3620819 7210.040473 TG(56:7) 1252.568854 508.4789411 6509.644135 TG(56:6) 0 0 0 TG(56:5) 284.9019531 147.1336952 1739.794278 TG(56:4) 155.4582697 90.57742945 937.0795219 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Formula Mass MW structure Mass Info (precursor ion, product ion) Retention time Human Metabolome Database InChIKey LipidMAPS quantified m/z DG(28:3) C31H54O5 506.3971 524.5 / 489.5 0.82 524.5 DG(28:2) C31H56O5 508.4128 526.5 / 491.5 1.02 HMDB0007038 UOMACDOEROJUON-XRZSNXEXSA-N LMGL02010325 526.5 DG(30:4) C33H56O5 532.412775 550.5 / 515.5 0.69 550.5 DG(30:3) C33H58O5 534.428425 552.5 / 517.5 1.04 552.5 DG(30:2) C33H60O5 536.4441 554.5 / 519.5 1.35 554.5 DG(32:3) C35H62O5 562.4597 580.5 / 545.5 0.9 580.5 DG(32:2) C35H64O5 564.4754 582.5 / 547.5 1.1 HMDB0007128 HSQHRRHRYJNSOC-DWCRPSDDSA-N LMGL02010011 582.5 DG(32:1) C35H66O5 566.491 584.5 / 549.5 1.34 584.5 DG(32:0) C35H68O5 568.5067 586.5 / 551.5 1.68 586.5 DG(34:4) C37H64O5 588.4754 606.5 / 571.5 1.12 606.5 DG(34:3) C37H66O5 590.491 608.5 / 573.5 1.16 608.5 DG(34:2) C37H68O5 592.5067 610.5 / 575.5 1.48 610.5 DG(34:1) C37H70O5 594.5223 612.5 / 577.5 1.76 HMDB0007102 YEJYLHKQOBOSCP-OZKTZCCCSA-N LMGL02010006 612.5 DG(34:0) C37H72O5 596.538 614.5 / 579.5 2.12 HMDB0056010 BFTGWUUHOMAGPO-PGUFJCEWSA-N 614.5 DG(36:5) C39H66O5 614.491 632.5 / 597.5 1.08 632.5 DG(36:4) C39H68O5 616.5067 634.5 / 599.5 1.26 634.5 DG(36:3) C39H70O5 618.5223 636.5 / 601.5 1.52 HMDB0007219 BLZVZPYMHLXLHG-JOBMVARSSA-N LMGL02010056 636.5 DG(36:2) C39H72O5 620.538 638.5 / 603.5 1.82 HMDB0007218 AFSHUZFNMVJNKX-LLWMBOQKSA-N LMGL02010049 638.5 DG(36:1) C39H74O5 622.5536 640.5 / 605.5 2.22 640.5 DG(36:0) C39H76O5 624.5693 642.5 / 607.5 2.62 642.5 DG(38:6) C41H68O5 640.5067 658.5 / 623.5 1.2 HMDB0007314 YDVDXUYJFQLPEG-CTHJWPIASA-N LMGL02010130 658.5 DG(38:5) C41H70O5 642.5223 660.5 / 625.5 1.44 HMDB0007312 GRGDLDNREYVILP-CNWVQWJYSA-N LMGL02010119 660.5 DG(38:4) C41H72O5 644.538 662.5 / 627.5 1.64 662.5 DG(38:3) C41H74O5 646.5536 664.5 / 629.5 1.94 HMDB0007310 KYOOZXPNLNVRQD-CVALLSOGSA-N LMGL02010098 664.5 DG(38:2) C41H76O5 648.5693 666.5 / 631.5 2.31 666.5 DG(38:1) C41H78O5 650.5849 668.5 / 633.5 2.67 668.5 DG(38:0) C41H80O5 652.6006 670.5 / 635.5 3.18 670.5 DG(40:7) C43H70O5 666.5223 684.6 / 649.6 0.82 684.6 DG(40:6) C43H72O5 668.538 686.6 / 651.6 1.17 HMDB0007179 LBDXVTOFXXDOGH-KXYFHQNYSA-N LMGL02010216 686.6 DG(40:4) C43H76O5 672.5693 690.6 / 655.6 1.99 HMDB0007373 OUGXERRDRINUQU-AICYSBPGSA-N LMGL02010165 690.6 TG(48:4) C51H90O6 798.6737 816.7 / 816.7 3.84 816.7 TG(48:3) C51H92O6 800.6894 818.7 / 818.7 4.18 HMDB0005432 SKGWNZXOCSYJQL-BUTYCLJRSA-N LMGL03010020 818.7 TG(48:2) C51H94O6 802.705 820.7 / 820.7 4.59 HMDB0005376 RUOVJPPUXXFZPC-YZEIBMOJSA-N LMGL03010018 820.7 TG(48:1) C51H96O6 804.7207 822.7 / 822.7 4.97 HMDB0005359 FEKLSEFRUGWUOS-DLOIZKPKSA-N LMGL03010017 822.7 TG(48:0) C51H98O6 806.7363 824.7 / 824.7 5.35 HMDB0005356 PVNIQBQSYATKKL-UHFFFAOYSA-N LMGL03010001 824.7 TG(50:4) C53H94O6 826.705 844.7 / 844.7 4.32 HMDB0005435 PVMBAGXWHHZKFP-JMPJWMFJSA-N LMGL03010064 844.7 TG(50:3) C53H96O6 828.7207 846.7 / 846.7 4.67 HMDB0005433 UFHNZOACKFBCOM-YXKNDSBASA-N LMGL03010052 846.7 TG(50:2) C53H98O6 830.7363 848.7 / 848.7 5.03 HMDB0005430 QEZWFCZNHWUARW-XQCAQTCHSA-N LMGL03010042 848.7 TG(50:1) C53H100O6 832.752 850.7 / 850.7 5.39 HMDB0005360 YHMDGPZOSGBQRH-YYSBDVFPSA-N LMGL03010006 850.7 TG(50:0) C53H102O6 834.7676 852.7 / 852.7 5.77 HMDB0043913 QRJMBNGGFSPTQP-UHFFFAOYSA-N 852.7 TG(52:6) C55H94O6 850.705 868.7 / 868.7 4.05 868.7 TG(52:5) C55H96O6 852.7207 870.7 / 870.7 4.43 HMDB0005446 CQZAAIKPSLHIBC-KDJOUNIJSA-N LMGL03010163 870.7 TG(52:4) C55H98O6 854.7363 872.7 / 872.7 4.75 HMDB0005440 WHSWXEYWNPTUPW-HNJDVRDNSA-N LMGL03010142 872.7 TG(52:3) C55H100O6 856.752 874.7 / 874.7 5.09 KGLAHZTWGPHKFF-FBSASISJSA-N 874.7 TG(52:2) C55H102O6 858.7676 876.7 / 876.7 5.43 JFISYPWOVQNHLS-NBHCHVEOSA-N 876.7 TG(52:1) C55H104O6 860.7833 878.7 / 878.7 5.8 HMDB0005367 NPCZZYKITFKRQZ-RFBIWTDZSA-N 878.7 TG(54:7) C57H96O6 876.7207 894.7 / 894.7 4.26 894.7 TG(54:6) C57H98O6 878.7363 896.7 / 896.7 4.49 HMDB0005474 HBOQXIRUPVQLKX-BBWANDEASA-N LMGL03010371 896.7 TG(54:5) C57H100O6 880.752 898.7 / 898.7 4.83 HMDB0005461 VVEBTVMJPTZDHO-YQFZQLSNSA-N LMGL03010327 898.7 TG(54:4) C57H102O6 882.7676 900.7 / 900.7 5.15 BRLGHZXETDWABO-NOFIOOQLSA-N LMGL03010291 900.7 TG(54:3) C57H104O6 884.7833 902.7 / 902.7 5.49 HMDB0005453 PHYFQTYBJUILEZ-IUPFWZBJSA-N LMGL03010250 902.7 TG(54:2) C57H106O6 886.7989 904.7 / 904.7 5.85 HMDB0031144 RYNHWWNZNIGDAQ-WGSDILPMSA-N 904.7 TG(56:10) C59H94O6 898.705 920.7 / 920.7 4.57 920.7 TG(56:9) C59H96O6 900.7207 922.7 / 922.7 4.71 922.7 TG(56:8) C59H98O6 902.7363 924.7 / 924.7 5.07 HMDB0005392 UBGUHMDKBGQUND-VPFWBQFRSA-N LMGL03010801 924.7 TG(56:7) C59H100O6 904.752 926.7 / 926.7 5.42 HMDB0010492 DODZUDCYRVWEOJ-GKZBLMSTSA-N LMGL03010734 926.7 TG(56:6) C59H102O6 906.7676 928.7 / 928.7 6.28 HMDB0010491 ZTNDRFCABXFVMY-WJTCTALZSA-N LMGL03010663 928.7 TG(56:5) C59H104O6 908.7833 930.7 / 930.7 5.89 HMDB0005473 UHEJWASONFIROS-YPSHDQQVSA-N LMGL03010596 930.7 TG(56:4) C59H106O6 910.7989 932.7 / 932.7 5.44 HMDB0005460 YONCDTJKIZDSKQ-IYASBODOSA-N LMGL03010538 932.7 METABOLITES_END #END