#METABOLOMICS WORKBENCH hatalbott2_20191118_161906 DATATRACK_ID:1852 STUDY_ID:ST001286 ANALYSIS_ID:AN002132 PROJECT_ID:PR000868
VERSION             	1
CREATED_ON             	December 12, 2019, 5:08 pm
#PROJECT
PR:PROJECT_TITLE                 	Lipid composition of isolated lipid droplets from the functional bovine corpus
PR:PROJECT_TITLE                 	luteum
PR:PROJECT_TYPE                  	lipidomics
PR:PROJECT_SUMMARY               	Establishment and maintenance of pregnancy is dependent on progesterone
PR:PROJECT_SUMMARY               	synthesized by the corpus luteum (CL). The CL is known for the prominent
PR:PROJECT_SUMMARY               	presence of intracellular lipid droplets (LDs). However relatively little is
PR:PROJECT_SUMMARY               	known about the composition and function of these luteal LDs. Our objective was
PR:PROJECT_SUMMARY               	to identify the lipid composition of LDs from fully functional bovine CLs.
PR:PROJECT_SUMMARY               	Luteal LDs were isolated by flotation through a discontinuous sucrose gradient,
PR:PROJECT_SUMMARY               	lipids were then extracted using a standard Bligh and Dyer protocol, dried, and
PR:PROJECT_SUMMARY               	sent to Avanti Polar Lipids for lipidomics analysis. The samples were provided
PR:PROJECT_SUMMARY               	for lipidomic profiling of free sterols, cholesteryl esters, triglycerides,
PR:PROJECT_SUMMARY               	diacylglycerols, phospholipids, and sphingolipids. Molecular species were
PR:PROJECT_SUMMARY               	resolved by reversed-phase liquid chromatography in the presence of class and
PR:PROJECT_SUMMARY               	sub-class specific internal standard compounds added to each sample. The
PR:PROJECT_SUMMARY               	compounds were detected by tandem mass spectrometry (MS/MS) with scheduled
PR:PROJECT_SUMMARY               	multiple reaction monitoring (MRM) for mass-specific fragment ions according to
PR:PROJECT_SUMMARY               	the lipid class and molecular weight of the compound. Quantification of
PR:PROJECT_SUMMARY               	cholesterol, cholesteryl esters, triglycerides, and diglycerides were directly
PR:PROJECT_SUMMARY               	calculated with standards and internal standards from calibration response
PR:PROJECT_SUMMARY               	curves. The remaining lipid species were semi-quantization using the integrated
PR:PROJECT_SUMMARY               	area of each analyte’s MRM peak, divided by the appropriate internal standard
PR:PROJECT_SUMMARY               	peak area, and multiplied by the standard’s known concentration. Lipid
PR:PROJECT_SUMMARY               	concentrations were normalized to the corresponding protein concentration of
PR:PROJECT_SUMMARY               	each sample and as a mol % relative to total lipids or within each lipid class.
PR:PROJECT_SUMMARY               	Isolated luteal LDs were composed primarily of triglyceride (88%, mol% of lipid
PR:PROJECT_SUMMARY               	class to total lipids). Other neutral lipids included diacylglycerol, 2.9%; and
PR:PROJECT_SUMMARY               	cholesteryl esters, 1.5%. Polar lipids were primarily composed of
PR:PROJECT_SUMMARY               	phosphatidylcholine (3.1%), sphingomyelin (1.5%), phosphatidylinositol (0.9%),
PR:PROJECT_SUMMARY               	phosphatidylethanolamine (0.8%) and phosphatidylserine (0.4%). A number of other
PR:PROJECT_SUMMARY               	minor lipids representing less than 0.32% of the total lipid pool were also
PR:PROJECT_SUMMARY               	detected including phosphatidylglycerol, lysophospholipids, ceramides, and
PR:PROJECT_SUMMARY               	glycosylated ceramides. Lipid composition of bovine luteal LDs are distinct from
PR:PROJECT_SUMMARY               	LDs isolated from other tissues and in other species.
PR:INSTITUTE                     	University of Nebraska Medical Center
PR:DEPARTMENT                    	Obstetrics and Gynecology
PR:LABORATORY                    	John S. Davis
PR:LAST_NAME                     	Davis
PR:FIRST_NAME                    	John
PR:ADDRESS                       	983255 Nebraska Medical Center Omaha, NE 68198-3255
PR:EMAIL                         	jsdavis@unmc.edu
PR:PHONE                         	402-599-9079
PR:FUNDING_SOURCE                	INBRE - P20GM103427-14, COBRE - 1P30GM110768-01
PR:CONTRIBUTORS                  	Heather Talbott, Xiaoying Hou, Crystal Cordes
#STUDY
ST:STUDY_TITLE                   	Lipid composition of isolated lipid droplets from the functional bovine corpus
ST:STUDY_TITLE                   	luteum
ST:STUDY_TYPE                    	Lipidomics
ST:STUDY_SUMMARY                 	Establishment and maintenance of pregnancy is dependent on progesterone
ST:STUDY_SUMMARY                 	synthesized by the corpus luteum (CL). The CL is known for the prominent
ST:STUDY_SUMMARY                 	presence of intracellular lipid droplets (LDs). However relatively little is
ST:STUDY_SUMMARY                 	known about the composition and function of these luteal LDs. Our objective was
ST:STUDY_SUMMARY                 	to identify the lipid composition of LDs from fully functional bovine CLs.
ST:STUDY_SUMMARY                 	Luteal LDs were isolated by flotation through a discontinuous sucrose gradient,
ST:STUDY_SUMMARY                 	lipids were then extracted using a standard Bligh and Dyer protocol, dried, and
ST:STUDY_SUMMARY                 	sent to Avanti Polar Lipids for lipidomics analysis. The samples were provided
ST:STUDY_SUMMARY                 	for lipidomic profiling of free sterols, cholesteryl esters, triglycerides,
ST:STUDY_SUMMARY                 	diacylglycerols, phospholipids, and sphingolipids. Molecular species were
ST:STUDY_SUMMARY                 	resolved by reversed-phase liquid chromatography in the presence of class and
ST:STUDY_SUMMARY                 	sub-class specific internal standard compounds added to each sample. The
ST:STUDY_SUMMARY                 	compounds were detected by tandem mass spectrometry (MS/MS) with scheduled
ST:STUDY_SUMMARY                 	multiple reaction monitoring (MRM) for mass-specific fragment ions according to
ST:STUDY_SUMMARY                 	the lipid class and molecular weight of the compound. Quantification of
ST:STUDY_SUMMARY                 	cholesterol, cholesteryl esters, triglycerides, and diglycerides were directly
ST:STUDY_SUMMARY                 	calculated with standards and internal standards from calibration response
ST:STUDY_SUMMARY                 	curves. The remaining lipid species were semi-quantization using the integrated
ST:STUDY_SUMMARY                 	area of each analyte’s MRM peak, divided by the appropriate internal standard
ST:STUDY_SUMMARY                 	peak area, and multiplied by the standard’s known concentration. Lipid
ST:STUDY_SUMMARY                 	concentrations were normalized to the corresponding protein concentration of
ST:STUDY_SUMMARY                 	each sample and as a mol % relative to total lipids or within each lipid class.
ST:STUDY_SUMMARY                 	Isolated luteal LDs were composed primarily of triglyceride (88%, mol% of lipid
ST:STUDY_SUMMARY                 	class to total lipids). Other neutral lipids included diacylglycerol, 2.9%; and
ST:STUDY_SUMMARY                 	cholesteryl esters, 1.5%. Polar lipids were primarily composed of
ST:STUDY_SUMMARY                 	phosphatidylcholine (3.1%), sphingomyelin (1.5%), phosphatidylinositol (0.9%),
ST:STUDY_SUMMARY                 	phosphatidylethanolamine (0.8%) and phosphatidylserine (0.4%). A number of other
ST:STUDY_SUMMARY                 	minor lipids representing less than 0.32% of the total lipid pool were also
ST:STUDY_SUMMARY                 	detected including phosphatidylglycerol, lysophospholipids, ceramides, and
ST:STUDY_SUMMARY                 	glycosylated ceramides. Lipid composition of bovine luteal LDs are distinct from
ST:STUDY_SUMMARY                 	LDs isolated from other tissues and in other species.
ST:INSTITUTE                     	University of Nebraska Medical Center
ST:DEPARTMENT                    	Obstetrics and Gynecology
ST:LABORATORY                    	John S. Davis
ST:LAST_NAME                     	Davis
ST:FIRST_NAME                    	John
ST:ADDRESS                       	983255 Nebraska Medical Center Omaha, NE 68198-3255
ST:EMAIL                         	jsdavis@unmc.edu
ST:PHONE                         	402-559-9079
ST:NUM_GROUPS                    	1
ST:TOTAL_SUBJECTS                	3
ST:NUM_FEMALES                   	3
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Bos taurus
SU:TAXONOMY_ID                   	9913
SU:GENDER                        	Female
SU:ANIMAL_ANIMAL_SUPPLIER        	JBS Beef Plant 3435 Edward Babe Gomez Ave, Omaha, NE 68107
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	bovine_CL_LD_replicate1	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	bovine_CL_LD_replicate2	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	bovine_CL_LD_replicate3	Treatment:Control	
#COLLECTION
CO:COLLECTION_SUMMARY            	Tissue (~2.5 g) was washed thoroughly in TE buffer (10 mM Tris, 1 mM EDTA, pH
CO:COLLECTION_SUMMARY            	7.4). Minced tissue was resuspended in 10 mL tissue homogenate buffer (60%
CO:COLLECTION_SUMMARY            	sucrose w/v in TE buffer containing protease and phosphatase inhibitor
CO:COLLECTION_SUMMARY            	cocktails) and homogenized with a Teflon Dounce homogenizer in a glass vessel.
CO:COLLECTION_SUMMARY            	The post-nuclear supernatant (PNS) fraction was obtained after centrifugation at
CO:COLLECTION_SUMMARY            	2000 rcf for 10 min. The supernatant was loaded into a 30 mL ultracentrifuge
CO:COLLECTION_SUMMARY            	tube and overlaid sequentially with 40%, 25%, 10%, and 0% sucrose w/v in TE
CO:COLLECTION_SUMMARY            	buffer containing protease and phosphatase inhibitor cocktails. Samples were
CO:COLLECTION_SUMMARY            	centrifuged at 110,000 × g (ravg) for 30 min at 4 °C with no brake in a
CO:COLLECTION_SUMMARY            	Beckman Coulter Avanti J-20 XP ultracentrifuge using an SW 32 Ti rotor. The LDs
CO:COLLECTION_SUMMARY            	concentrated in a yellow-ish band at the top of the gradient were harvested and
CO:COLLECTION_SUMMARY            	concentrated by centrifugation at 2000 rcf for 10 min at 4 °C. This protocol
CO:COLLECTION_SUMMARY            	was derived from Ding et al. 2012, and Brasaemale et al. 2016. Ding, Y., Zhang,
CO:COLLECTION_SUMMARY            	S., Yang, L., Na, H., Zhang, P., Zhang, H., … Liu, P. (2013). Isolating lipid
CO:COLLECTION_SUMMARY            	droplets from multiple species. Nature Protocols, 8(1), 43–51.
CO:COLLECTION_SUMMARY            	https://doi.org/10.1038/nprot.2012.142 Brasaemle, D. L., & Wolins, N. E. (2016).
CO:COLLECTION_SUMMARY            	Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation.
CO:COLLECTION_SUMMARY            	Current Protocols in Cell Biology, 72, 3.15.1-3.15.13.
CO:COLLECTION_SUMMARY            	https://doi.org/10.1002/cpcb.10
CO:SAMPLE_TYPE                   	Ovary
CO:VOLUMEORAMOUNT_COLLECTED      	2.5 g of corpus luteum tissue
#TREATMENT
TR:TREATMENT_SUMMARY             	N/A
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipids from CL tissue LDs (~250uL) were extracted using a standard Bligh and
SP:SAMPLEPREP_SUMMARY            	Dyer extraction protocol and then dried and sent to Avanti Polar Lipids for
SP:SAMPLEPREP_SUMMARY            	lipidomics analysis. Extracts were received as dried residues in glass vials and
SP:SAMPLEPREP_SUMMARY            	were immediately stored at -80 °C until analysis. Bligh, E. G., & Dyer, W. J.
SP:SAMPLEPREP_SUMMARY            	(1959). A rapid method of total lipid extraction and purification. Canadian
SP:SAMPLEPREP_SUMMARY            	Journal of Biochemistry and Physiology, 37(8), 911–917.
SP:SAMPLEPREP_SUMMARY            	https://doi.org/10.1139/o59-099
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACTION_METHOD             	Bligh & Dyer, chloroform:methanol (1:2, v:v)
SP:EXTRACT_STORAGE               	-80℃
SP:SAMPLE_RESUSPENSION           	1mL of chloroform:methanol (8:2, v/v)
SP:SAMPLE_DERIVATIZATION         	N/A
SP:SUBCELLULAR_LOCATION          	Lipid Droplet
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Molecular species were resolved by reversed-phase liquid chromatography in the
CH:CHROMATOGRAPHY_SUMMARY        	presence of class and sub-class specific internal standard compounds added to
CH:CHROMATOGRAPHY_SUMMARY        	each sample. Selectivity was further enhanced by scheduling the detection of
CH:CHROMATOGRAPHY_SUMMARY        	each compound according to its elution from the high-performance liquid
CH:CHROMATOGRAPHY_SUMMARY        	chromatography (HPLC) column, known as scheduled MRM (sMRM).
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um)
CH:INTERNAL_STANDARD             	LPC(17:0), PC(37:4), LPE(17:1), PE(37:4)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Avanti Polar Lipids, Inc
AN:DETECTOR_TYPE                 	AcQuRate™ Pulse Counting CEM
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 5500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	sMRM NL 141 u
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	nM
MS_METABOLITE_DATA_START
Samples	bovine_CL_LD_replicate1	bovine_CL_LD_replicate2	bovine_CL_LD_replicate3
Factors	Treatment:Control	Treatment:Control	Treatment:Control
LPE(14:0)	0	0	0
LPE(16:1)	0	0	0
LPE(16:0)	0.160997423	0	0.307575442
LPE(18:2)	0.038388387	0.010750288	0
LPE(18:1)	0.214005727	0.07610821	0.298776355
LPE(18:0)	0.311852799	0.102294593	0.947672659
LPE(20:5)	0.459596967	0.03247209	0.046295082
LPE(20:4)	1.955531359	0.823760512	1.385467169
LPE(20:3)	0	0	0.258954067
LPE(22:6)	0.018279992	0.029224511	0.042904147
LPE(22:4)	0.526944299	0.463812474	0.919818196
PE(30:1)	0.042640369	0	0.064924577
PE(32:1)	0	0	0
PE(P-34:4)/PE(O-34:5)	0	0	0.035155249
PE(P-34:3)/PE(O-34:4)	0.190942937	0.084045574	0.266039729
PE(P-34:2)/PE(O-34:3)	0	0.198465454	0.540552954
PE(P-34:1)/PE(O-34:2)	0.297399625	0.197511795	1.268267198
PE(34:3)	0.226600874	0	0.427168387
PE(34:2)	9.176464108	5.605800079	26.35213394
PE(34:1)	14.40651671	12.52838571	55.30879956
PE(34:0)	0.069016136	0	1.326045387
PE(P-36:5)/PE(O-36:6)	0	0	0
PE(P-36:4/PE(O-36:5)	0	0	0
PE(P-36:3/PE(O-36:4)	0	0	0
PE(P-36:2/PE(O-36:3)	0.025922533	0	0
PE(P-36:1)/PE(O-36:2)	0.011784289	0.023180699	0.014837153
PE(36:6)	1.012730043	0.319697497	1.838670386
PE(36:5)	0	0	1.444443536
PE(36:4)	20.56628307	14.9234799	58.73926053
PE(36:3)	8.452932496	5.758651461	18.94415706
PE(36:2)	34.61780956	16.68320514	56.8529757
PE(36:1)	11.40677076	9.469075974	42.06912491
PE(36:0)	0	0.03046267	0.049294773
PE(P-38:6/PE(O-38:7)	0	0	0
PE(P-38:5/PE(O-38:6)	0.993461582	1.258091469	3.336658829
PE(38:8)	0	0	0.008766106
PE(38:6)	0.679749151	0.573566264	1.489138554
PE(38:5)	5.305796316	3.885080507	17.59946913
PE(38:4)	42.79020793	17.60597821	52.91714249
PE(38:3)	2.019862195	3.423631301	8.914966295
PE(38:2)	1.209884517	1.382170962	6.200331853
PE(38:1)	0.513104976	0.698763658	2.367781949
PE(38:0)	0	0	0
PE(P-40:5/PE(O-40:6)	0	0	0
PE(P-40:4/PE(O-40:5)	0.488600297	0.249662744	1.511396131
PE(40:8)	1.627287257	1.40349245	4.67898773
PE(40:7)	5.089184392	3.856728502	10.05358059
PE(40:6)	0	0	0
PE(40:5)	1.518225458	1.704876542	6.063464252
PE(40:4)	1.417195878	2.37795408	5.618233111
PE(40:1)	0.063380322	0.033437563	0.077500937
PE(P-42:5)/PE(O-42:6)	0	0	0
PE(P-42:4)/PE(O-42:5)	0	0	0
PE(42:8)	0.142280233	0.08191593	0.507189981
PE(42:6)	0.793618678	0.52600979	3.381039587
PE(42:5)	0.795043523	1.621781476	4.36010564
PE(42:4)	0.323244009	0.394586966	1.261451997
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Formula	Mass	MW structure	Mass Info (precursor ion, product ion)	Retention times	Human Metabolome Database	InChIKey	LipidMAPS	quantified m/z
LPE(14:0)	C5H14NO6P	215.0559		426.3 / 285.3	1.06	HMDB0011500	RPXHXZNGZBHSMJ-GOSISDBHSA-N	LMGP02050003	426.3
LPE(16:1)	C21H42NO7P	451.2699		452.3 / 311.3	1.06	HMDB0011474	DSOWUEHXZJUNID-WHXUGTBJSA-N	LMGP02050010	452.3
LPE(16:0)	C21H44NO7P	453.2855		454.3 / 313.3	1.16	HMDB0011503	YVYMBNSKXOXSKW-HXUWFJFHSA-N	LMGP02050002	454.3
LPE(18:2)	C23H44NO7P	477.2855		478.3 / 337.3	1.06	HMDB0011477	SVRBKLJIDJHADS-USWSLJGRSA-N		478.3
LPE(18:1)	C23H46NO7P	479.3012		480.3 / 339.3	1.5	HMDB0011506	PYVRVRFVLRNJLY-MZMPXXGTSA-N	LMGP02050004	480.3
LPE(18:0)	C23H48NO7P	481.3168		482.3 / 341.3	1.67	HMDB0011129	KIHAGWUUUHJRMS-JOCHJYFZSA-N		482.3
LPE(20:5)	C25H42NO7P	499.2699		500.3 / 359.3	0.95	HMDB0011489	MMHCCHGAKPRCIO-KOYQJJOGSA-N	LMGP02050027	500.3
LPE(20:4)	C25H44NO7P	501.2855		502.3 / 361.3	1.06	HMDB0011517	ROPRRXYVXLDXQO-XSQXPFHXSA-N	LMGP02050009	502.3
LPE(20:3)	C25H46NO7P	503.3012		504.3 / 363.3	1.27	HMDB0011485	FJDVENKXPUIXRG-WMTBOZPISA-N		504.3
LPE(22:6)	C27H44NO7P	525.2855		526.3 / 385.3	1.06	HMDB0011496	TWBVHOYVCUOMJY-PAUXXPOVSA-N	LMGP02050013	526.3
LPE(22:4)	C27H48NO7P	529.3168		530.3 / 389.3	1.38	HMDB0011523	NJHKGJJIKOCCMZ-YMIVKDKMSA-N	LMGP02050014	530.3
PE(30:1)	C35H68NO8P	661.4683		662.5 / 521.5	3.39	HMDB0008883			662.5
PE(32:1)	C37H72NO8P	689.4996		690.5 / 529.5	3.81	HMDB0009052	MFWTZMQSTYTELL-XHYHITGYSA-N	LMGP02011199	690.5
PE(P-34:4)/PE(O-34:5)	C39H70NO7P	695.489		698.5 / 557.5	3.49				698.5
PE(P-34:3)/PE(O-34:4)	C39H72NO7P	697.5046		700.5 / 559.5	3.71				700.5
PE(P-34:2)/PE(O-34:3)	C39H74NO7P	699.5203		702.5 / 561.5	3.66				702.5
PE(P-34:1)/PE(O-34:2)	C39H76NO7P	701.5359		704.5 / 563.5	3.71				704.5
PE(34:3)	C39H72NO8P	713.4996		714.5 / 573.5	3.39	HMDB0008930			714.5
PE(34:2)	C39H74NO8P	715.5152		716.5 / 575.5	3.49	HMDB0008928	HBZNVZIRJWODIB-NHCUFCNUSA-N	LMGP02010042	716.5
PE(34:1)	C39H76NO8P	717.5309		718.5 / 577.5	3.71	HMDB0008927	FHQVHHIBKUMWTI-OTMQOFQLSA-N	LMGP02010009	718.5
PE(34:0)	C39H78NO8P	719.5465		720.5 / 579.5	3.78	HMDB0008925	RPJZYOHZALDGKI-DIPNUNPCSA-N	LMGP02011225	720.5
PE(P-36:5)/PE(O-36:6)	C41H72NO7P	721.5046		724.5 / 583.5	3.71				724.5
PE(P-36:4/PE(O-36:5)	C41H74NO7P	723.520292		726.5 / 585.5	3.71				726.5
PE(P-36:3/PE(O-36:4)	C41H76NO7P	725.535942		728.5 / 587.5	3.71				728.5
PE(P-36:2/PE(O-36:3)	C41H78NO7P	727.551592		730.5 / 589.5	3.92				730.5
PE(P-36:1)/PE(O-36:2)	C41H80NO7P	729.5672		732.6 / 591.5	4.11				732.6
PE(36:6)	C41H70NO8P	735.4839		736.5 / 595.5	3.28				736.5
PE(36:5)	C41H72NO8P	737.4996		738.5 / 597.5	3.35	HMDB0009095			738.5
PE(36:4)	C41H74NO8P	739.5152		740.5 / 599.5	3.49	HMDB0008937	DRIVXEVMDWCWLI-CAQMIEAISA-N	LMGP02010096	740.5
PE(36:3)	C41H76NO8P	741.5309		742.5 / 601.5	3.6	HMDB0008936	LMWFNZUKABEGHS-CISNCOODSA-N	LMGP02011222	742.5
PE(36:2)	C41H78NO8P	743.5465		744.5 / 603.5	3.71	HMDB0008994	YDTWOEYVDRKKCR-KNERPIHHSA-N	LMGP02010044	744.5
PE(36:1)	C41H80NO8P	745.5622		746.6 / 605.6	3.92		JQKOHRZNEOQNJE-ZZEZOPTASA-N		746.6
PE(36:0)	C41H82NO8P	747.5778		748.5 / 607.6	3.98	HMDB0008991	LVNGJLRDBYCPGB-LDLOPFEMSA-N	LMGP02010097	748.5
PE(P-38:6/PE(O-38:7)	C43H74NO7P	747.520292		750.5 / 609.6	3.71				750.5
PE(P-38:5/PE(O-38:6)	C43H76NO7P	749.535942		752.5 / 611.6	3.8				752.5
PE(38:8)	C43H70NO8P	759.4839		760.5 / 619.6	3.37	HMDB0009170			760.5
PE(38:6)	C43H74NO8P	763.5152		764.5 / 623.6	3.49	HMDB0009102	LFGBKOUQHCWBQI-BZGLIJSBSA-N	LMGP02011192	764.5
PE(38:5)	C43H76NO8P	765.5309		766.5 / 625.6	3.6	HMDB0009069	PECSWFQRRFRZPW-BHPGJWMBSA-N	LMGP02011196	766.5
PE(38:4)	C43H78NO8P	767.5465		768.6 / 627.6	3.71	HMDB0009003	ANRKEHNWXKCXDB-BHFWLYLHSA-N	LMGP02010118	768.6
PE(38:3)	C43H80NO8P	769.5622		770.6 / 629.6	3.67	HMDB0009130	PCKHTZYYWAQHBJ-LNQXFAROSA-N		770.6
PE(38:2)	C43H82NO8P	771.5778		772.6 / 631.6	3.9	HMDB0009225	CLPMAPXZURYSNH-CNBLIUODSA-N	LMGP02010125	772.6
PE(38:1)	C43H84NO8P	773.5935		774.6 / 633.6	4.06	HMDB0008941			774.6
PE(38:0)	C43H86NO8P	775.6091		776.6 / 635.6	4.23	HMDB0008998	TZINTCMFTOUPSN-VQJSHJPSSA-N		776.6
PE(P-40:5/PE(O-40:6)	C45H80NO7P	777.567242		778.6 / 637.6	3.81				778.6
PE(P-40:4/PE(O-40:5)	C45H82NO7P	779.582892		780.6 / 639.6	3.92				780.6
PE(40:8)	C45H74NO8P	787.5152		788.6 / 647.6	3.33	HMDB0009207	HMUNESAIQZELST-AQPMPDAQSA-N	LMGP02010767	788.6
PE(40:7)	C45H76NO8P	789.5309		790.6 / 649.6	3.6	HMDB0009367			790.6
PE(40:6)	C45H78NO8P	791.5465		792.6 / 651.6	3.71	HMDB0009012	XYYHNDVKALDFHQ-OXHZBIAZSA-N	LMGP02010094	792.6
PE(40:5)	C45H80NO8P	793.5622		794.6 / 653.6	3.7	HMDB0009010	DPRXQVIZBLENFV-NPQRSGSQSA-N		794.6
PE(40:4)	C45H82NO8P	795.5778		796.6 / 655.6	3.81	HMDB0009009	HNFSZKRUPTYBOQ-ZDYOIFAYSA-N	LMGP02011200	796.6
PE(40:1)	C45H88NO8P	801.6248		802.6 / 661.6	4.02				802.6
PE(P-42:5)/PE(O-42:6)	C47H84NO7P	805.5985		806.6 / 665.6	4.02				806.6
PE(P-42:4)/PE(O-42:5)	C47H86NO7P	807.6142		808.6 / 667.6	3.49				808.6
PE(42:8)	C47H78NO8P	815.5465		814.6 / 673.6	3.07	HMDB0009405	IAMDYOOTWAQMME-DVRHFKRZSA-N	LMGP02010960	814.6
PE(42:6)	C47H82NO8P	819.5778		818.6 / 677.6	3.6	HMDB0009659	XLYAEBCMTCDSEA-NIVNKCBGSA-N	LMGP02010959	818.6
PE(42:5)	C47H84NO8P	821.5935		820.6 / 679.6	3.92	HMDB0009212	ZNNFJVNPRVPYIW-RBEXFCIKSA-N		820.6
PE(42:4)	C47H86NO8P	823.6091		822.6 / 681.6	3.88	HMDB0009179			822.6
METABOLITES_END
#END