#METABOLOMICS WORKBENCH efahy_20191217_062906 DATATRACK_ID:1873 STUDY_ID:ST001288 ANALYSIS_ID:AN002141 PROJECT_ID:PR000870 VERSION 1 CREATED_ON December 17, 2019, 7:20 am #PROJECT PR:PROJECT_TITLE Subcellular organelle lipidomics in TLR-4-activated macrophages PR:PROJECT_SUMMARY Lipids orchestrate biological processes by acting remotely as signaling PR:PROJECT_SUMMARY molecules or locally as membrane components that modulate protein function. PR:PROJECT_SUMMARY Detailed insight into lipid function requires knowledge of the subcellular PR:PROJECT_SUMMARY localization of individual lipids. We report an analysis of the subcellular PR:PROJECT_SUMMARY lipidome of the mammalian macrophage, a cell type that plays key roles in PR:PROJECT_SUMMARY inflammation, immune responses, and phagocytosis. Nuclei, mitochondria, PR:PROJECT_SUMMARY endoplasmic reticulum (ER), plasmalemma, and cytoplasm were isolated from RAW PR:PROJECT_SUMMARY 264.7 macrophages in basal and activated states. Subsequent lipidomic analyses PR:PROJECT_SUMMARY of major membrane lipid categories identified 229 individual/isobaric species, PR:PROJECT_SUMMARY including 163 glycerophospholipids, 48 sphingolipids, 13 sterols, and 5 prenols. PR:PROJECT_SUMMARY Major subcellular compartments exhibited substantially divergent PR:PROJECT_SUMMARY glycerophospholipid profiles. Activation of macrophages by the Toll-like PR:PROJECT_SUMMARY receptor 4-specific lipopolysaccharide Kdo2-lipid A caused significant PR:PROJECT_SUMMARY remodeling of the subcellular lipidome. Some changes in lipid composition PR:PROJECT_SUMMARY occurred in all compartments (e.g. increases in the levels of ceramides and the PR:PROJECT_SUMMARY cholesterol precursors desmosterol and lanosterol). Other changes were manifest PR:PROJECT_SUMMARY in specific organelles. For example, oxidized sterols increased and unsaturated PR:PROJECT_SUMMARY cardiolipins decreased in mitochondria, whereas unsaturated ether-linked PR:PROJECT_SUMMARY phosphatidylethanolamines decreased in the ER. We speculate that these changes PR:PROJECT_SUMMARY may reflect mitochondrial oxidative stress and the release of arachidonic acid PR:PROJECT_SUMMARY from the ER in response to cell activation. PR:INSTITUTE University of California, San Diego PR:DEPARTMENT Bioengineering PR:LAST_NAME Fahy PR:FIRST_NAME Eoin PR:ADDRESS 9500 Gilman, La Jolla, CA, 92093, USA PR:EMAIL efahy@ucsd.edu PR:PHONE 858-534-4076 #STUDY ST:STUDY_TITLE Subcellular organelle lipidomics in TLR-4-activated macrophages ST:STUDY_SUMMARY Lipids orchestrate biological processes by acting remotely as signaling ST:STUDY_SUMMARY molecules or locally as membrane components that modulate protein function. ST:STUDY_SUMMARY Detailed insight into lipid function requires knowledge of the subcellular ST:STUDY_SUMMARY localization of individual lipids. We report an analysis of the subcellular ST:STUDY_SUMMARY lipidome of the mammalian macrophage, a cell type that plays key roles in ST:STUDY_SUMMARY inflammation, immune responses, and phagocytosis. Nuclei, mitochondria, ST:STUDY_SUMMARY endoplasmic reticulum (ER), plasmalemma, and cytoplasm were isolated from RAW ST:STUDY_SUMMARY 264.7 macrophages in basal and activated states. Subsequent lipidomic analyses ST:STUDY_SUMMARY of major membrane lipid categories identified 229 individual/isobaric species, ST:STUDY_SUMMARY including 163 glycerophospholipids, 48 sphingolipids, 13 sterols, and 5 prenols. ST:STUDY_SUMMARY Major subcellular compartments exhibited substantially divergent ST:STUDY_SUMMARY glycerophospholipid profiles. Activation of macrophages by the Toll-like ST:STUDY_SUMMARY receptor 4-specific lipopolysaccharide Kdo2-lipid A caused significant ST:STUDY_SUMMARY remodeling of the subcellular lipidome. Some changes in lipid composition ST:STUDY_SUMMARY occurred in all compartments (e.g. increases in the levels of ceramides and the ST:STUDY_SUMMARY cholesterol precursors desmosterol and lanosterol). Other changes were manifest ST:STUDY_SUMMARY in specific organelles. For example, oxidized sterols increased and unsaturated ST:STUDY_SUMMARY cardiolipins decreased in mitochondria, whereas unsaturated ether-linked ST:STUDY_SUMMARY phosphatidylethanolamines decreased in the ER. We speculate that these changes ST:STUDY_SUMMARY may reflect mitochondrial oxidative stress and the release of arachidonic acid ST:STUDY_SUMMARY from the ER in response to cell activation. ST:INSTITUTE LIPID MAPS ST:DEPARTMENT Multiple ST:LABORATORY Multiple ST:LAST_NAME Fahy ST:FIRST_NAME Eoin ST:ADDRESS 9500 Gilman, La Jolla, CA, 92093, USA ST:EMAIL efahy@ucsd.edu ST:PHONE 858-534-4076 ST:PUBLICATIONS Andreyev AY, Fahy E, Guan Z, Kelly S, Li X, McDonald JG, Milne S, Myers D, Park ST:PUBLICATIONS H, Ryan A, Thompson BM, Wang E, Zhao Y, Brown HA, Merrill AH, Raetz CR, Russell ST:PUBLICATIONS DW, Subramaniam S, Dennis EA. Subcellular organelle lipidomics in ST:PUBLICATIONS TLR-4-activated macrophages. J Lipid Res. 2010 Sep;51(9):2785-97. doi: 10.1194/jlr.M008748. Epub 2010 ST:PUBLICATIONS Jun 23. PMID: 20574076; PMCID: PMC2918461. #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - S1 Fraction:Nucleus | Treatment:Control SUBJECT_SAMPLE_FACTORS - S2 Fraction:Nucleus | Treatment:Control SUBJECT_SAMPLE_FACTORS - S3 Fraction:Nucleus | Treatment:Control SUBJECT_SAMPLE_FACTORS - S4 Fraction:Nucleus | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S5 Fraction:Nucleus | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S6 Fraction:Nucleus | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S7 Fraction:Mitochondria | Treatment:Control SUBJECT_SAMPLE_FACTORS - S8 Fraction:Mitochondria | Treatment:Control SUBJECT_SAMPLE_FACTORS - S9 Fraction:Mitochondria | Treatment:Control SUBJECT_SAMPLE_FACTORS - S10 Fraction:Mitochondria | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S11 Fraction:Mitochondria | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S12 Fraction:Mitochondria | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S13 Fraction:ER | Treatment:Control SUBJECT_SAMPLE_FACTORS - S14 Fraction:ER | Treatment:Control SUBJECT_SAMPLE_FACTORS - S15 Fraction:ER | Treatment:Control SUBJECT_SAMPLE_FACTORS - S16 Fraction:ER | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S17 Fraction:ER | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S18 Fraction:ER | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S19 Fraction:Plasma membrane | Treatment:Control SUBJECT_SAMPLE_FACTORS - S20 Fraction:Plasma membrane | Treatment:Control SUBJECT_SAMPLE_FACTORS - S21 Fraction:Plasma membrane | Treatment:Control SUBJECT_SAMPLE_FACTORS - S22 Fraction:Plasma membrane | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S23 Fraction:Plasma membrane | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S24 Fraction:Plasma membrane | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S25 Fraction:Dense microsomes | Treatment:Control SUBJECT_SAMPLE_FACTORS - S26 Fraction:Dense microsomes | Treatment:Control SUBJECT_SAMPLE_FACTORS - S27 Fraction:Dense microsomes | Treatment:Control SUBJECT_SAMPLE_FACTORS - S28 Fraction:Dense microsomes | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S29 Fraction:Dense microsomes | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S30 Fraction:Dense microsomes | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S31 Fraction:Cytosol | Treatment:Control SUBJECT_SAMPLE_FACTORS - S32 Fraction:Cytosol | Treatment:Control SUBJECT_SAMPLE_FACTORS - S33 Fraction:Cytosol | Treatment:Control SUBJECT_SAMPLE_FACTORS - S34 Fraction:Cytosol | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S35 Fraction:Cytosol | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S36 Fraction:Cytosol | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S37 Fraction:Whole cells | Treatment:Control SUBJECT_SAMPLE_FACTORS - S38 Fraction:Whole cells | Treatment:Control SUBJECT_SAMPLE_FACTORS - S39 Fraction:Whole cells | Treatment:Control SUBJECT_SAMPLE_FACTORS - S40 Fraction:Whole cells | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S41 Fraction:Whole cells | Treatment:KLA SUBJECT_SAMPLE_FACTORS - S42 Fraction:Whole cells | Treatment:KLA #COLLECTION CO:COLLECTION_SUMMARY Immortalized mouse macrophage-like RAW264.7 cells were obtained from the ATCC CO:COLLECTION_SUMMARY (catalog no. TIB-71). DMEM (catalog no. 10-013) and PBS (catalog no. 21-031-CV) CO:COLLECTION_SUMMARY were from Mediatech. Fetal calf serum with low endotoxin content was from CO:COLLECTION_SUMMARY Hyclone (SH30071.03 ANG19242). KLA and lipid standards were from Avanti Polar CO:COLLECTION_SUMMARY Lipids. Iodixanol (OptiPrep from Axis-Shield) was obtained from Sigma-Aldrich. CO:COLLECTION_SUMMARY Solvents were chromatography-grade and purchased from OmniSolv. All other CO:COLLECTION_SUMMARY reagents/kits were from Sigma-Aldrich. Aqueous solutions were prepared using CO:COLLECTION_SUMMARY distilled-deionized water (catalog no. 25-055-CV) from Mediatech. Isolation CO:COLLECTION_SUMMARY media were prepared K+- and Na+-free; pH was adjusted by addition of Tris base CO:COLLECTION_SUMMARY (TRIZMA). Cells were maintained, treated, and fractionated as previously CO:COLLECTION_SUMMARY described (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830848/). Briefly, CO:COLLECTION_SUMMARY RAW264.7 cells were maintained between passages 4 and 24 at 37°C and 10% CO2. CO:COLLECTION_SUMMARY The medium was composed of high glucose- and l-glutamine-containing DMEM CO:COLLECTION_SUMMARY supplemented with 10% heat-inactivated fetal calf serum, 100 units/ml CO:COLLECTION_SUMMARY penicillin, and 100 µg/ml streptomycin. For an experiment, five T-150 flasks of CO:COLLECTION_SUMMARY cells were plated at a density of 3.6 × 107 cells/flask in 24 ml of medium. At CO:COLLECTION_SUMMARY 24 h after plating, cells were treated with vehicle or 100 ng/ml KLA for another CO:COLLECTION_SUMMARY 24 h followed by subcellular fractionation. CO:SAMPLE_TYPE Macrophages #TREATMENT TR:TREATMENT_SUMMARY Subcellular fractionation procedures are described in detail in the publication TR:TREATMENT_SUMMARY doi: 10.1194/jlr.M008748 #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The various extraction procedures and lipidomic analyses for each lipid class SP:SAMPLEPREP_SUMMARY are described in the publication doi: 10.1194/jlr.M008748 #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY See publication for details doi: CH:CHROMATOGRAPHY_SUMMARY 10.1194/jlr.M008748 CH:CHROMATOGRAPHY_TYPE Reversed phase/Normal phase CH:INSTRUMENT_NAME Several CH:COLUMN_NAME Several #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Several MS:INSTRUMENT_TYPE Several MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS See publication for details. doi: MS:MS_COMMENTS 10.1194/jlr.M008748 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmoles per mg protein MS_METABOLITE_DATA_START Samples S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 S17 S18 S19 S20 S21 S22 S23 S24 S25 S26 S27 S28 S29 S30 S31 S32 S33 S34 S35 S36 S37 S38 S39 S40 S41 S42 Factors Fraction:Nucleus | Treatment:Control Fraction:Nucleus | Treatment:Control Fraction:Nucleus | Treatment:Control Fraction:Nucleus | Treatment:KLA Fraction:Nucleus | Treatment:KLA Fraction:Nucleus | Treatment:KLA Fraction:Mitochondria | Treatment:Control Fraction:Mitochondria | Treatment:Control Fraction:Mitochondria | Treatment:Control Fraction:Mitochondria | Treatment:KLA Fraction:Mitochondria | Treatment:KLA Fraction:Mitochondria | Treatment:KLA Fraction:ER | Treatment:Control Fraction:ER | Treatment:Control Fraction:ER | Treatment:Control Fraction:ER | Treatment:KLA Fraction:ER | Treatment:KLA Fraction:ER | Treatment:KLA Fraction:Plasma membrane | Treatment:Control Fraction:Plasma membrane | Treatment:Control Fraction:Plasma membrane | Treatment:Control Fraction:Plasma membrane | Treatment:KLA Fraction:Plasma membrane | Treatment:KLA Fraction:Plasma membrane | Treatment:KLA Fraction:Dense microsomes | Treatment:Control Fraction:Dense microsomes | Treatment:Control Fraction:Dense microsomes | Treatment:Control Fraction:Dense microsomes | Treatment:KLA Fraction:Dense microsomes | Treatment:KLA Fraction:Dense microsomes | Treatment:KLA Fraction:Cytosol | Treatment:Control Fraction:Cytosol | Treatment:Control Fraction:Cytosol | Treatment:Control Fraction:Cytosol | Treatment:KLA Fraction:Cytosol | Treatment:KLA Fraction:Cytosol | Treatment:KLA Fraction:Whole cells | Treatment:Control Fraction:Whole cells | Treatment:Control Fraction:Whole cells | Treatment:Control Fraction:Whole cells | Treatment:KLA Fraction:Whole cells | Treatment:KLA Fraction:Whole cells | Treatment:KLA PA(30:1) 54 74 110 100 110 150 66 130 100 110 130 84 130 100 79 180 110 140 87 180 110 140 120 230 69 130 160 150 190 180 62 110 100 95 110 150 43 42 41 41 68 PA(30:0) 190 160 120 130 280 120 190 330 180 300 320 130 180 43 44 34 PA(32:4) 130 89 54 72 110 33 51 63 69 PA(32:1) 25 30 47 100 62 120 140 49 160 110 270 230 180 110 230 220 250 230 53 58 67 54 30 35 37 46 39 26 PA(32:0) 140 240 260 300 250 110 120 920 300 1300 1100 65 510 1300 570 1500 790 130 1100 3100 2100 1200 1800 250 110 300 170 220 230 74 29 56 61 170 350 250 280 290 60 PA(34:2) 13 19 24 23 20 30 27 48 40 34 46 18 87 60 7.8 120 20 58 38 96 84 94 53 63 41 20 17 26 27 40 9 10 7 7.5 7 19 17 14 15 12 17 PA(34:1) 78 110 120 170 140 140 140 400 240 570 670 210 390 570 490 1100 890 660 460 940 850 1000 1100 670 170 220 210 270 300 170 27 39 33 57 64 63 110 140 120 180 160 110 PA(34:0) 90 180 170 190 130 83 100 600 230 670 570 44 340 750 310 860 400 68 680 2000 1300 600 900 72 190 120 94 120 45 21 25 110 250 180 180 150 34 PA(36:3) 72 55 100 56 97 98 95 16 PA(36:2) 56 120 160 170 170 230 200 160 260 250 170 110 330 420 210 600 270 510 320 600 530 640 660 360 170 240 210 190 220 230 61 86 71 92 82 160 85 46 58 54 55 110 PA(36:1) 110 150 160 220 200 240 190 470 290 660 700 300 890 900 840 2000 1300 1500 820 1600 1400 2200 2100 2000 270 310 370 410 440 360 37 68 51 84 95 91 150 110 180 260 260 230 PA(36:0) 31 50 61 69 54 63 40 170 89 250 200 44 170 220 180 340 250 200 230 510 380 380 390 64 70 51 63 29 45 10 39 74 59 75 60 45 PA(38:5) 57 65 130 120 140 150 65 130 96 100 120 84 90 110 110 150 91 220 170 170 140 120 130 100 93 110 110 45 67 70 53 71 110 60 50 37 44 43 PA(38:4) 23 51 49 64 53 50 76 240 210 330 200 110 200 370 300 320 88 96 84 33 50 23 25 48 PA(38:3) 32 48 73 68 54 29 250 77 330 240 270 390 290 600 310 330 310 940 670 490 670 490 38 140 81 130 59 25 36 25 59 90 93 91 68 74 PA(38:2) 32 47 88 65 110 38 51 48 95 90 160 65 24 36 280 260 29 13 27 26 51 PA(38:1) 54 73 74 110 92 73 190 130 150 150 21 23 30 24 PA(40:6) 110 120 290 450 200 400 520 950 750 840 410 720 710 810 710 190 230 230 220 110 100 140 160 PA(40:4) 83 86 190 120 250 350 150 300 230 720 380 470 300 570 430 660 650 150 150 62 82 71 82 120 99 92 PA(40:3) 49 56 58 250 81 320 260 330 220 580 290 240 300 890 560 520 560 89 71 85 79 91 80 22 PA(40:1) 190 120 130 PA(40:0) 130 180 170 160 170 280 320 310 240 29 39 PC(28:0) 70 100 120 250 170 260 130 280 280 540 780 280 730 610 360 730 470 430 270 1100 640 820 1200 1800 110 150 120 230 320 220 36 93 26 70 64 67 63 63 110 120 130 PC(32:2) 160 220 330 250 270 370 380 610 820 490 940 460 840 1000 1500 940 960 860 600 1000 1100 690 1200 340 470 680 460 590 430 62 130 91 100 130 120 240 130 180 120 140 160 PC(32:1) 930 1300 1900 2300 2300 3100 2100 3700 4000 3900 6800 3500 2900 7400 10000 9900 9000 7300 4600 8500 8500 8700 10000 7000 2400 3400 4300 4200 4700 3400 460 800 590 880 1100 960 1600 920 1100 1100 1200 1200 PC(32:0) 430 600 590 890 850 980 740 1600 1300 1600 2300 990 1700 2800 2800 3400 2900 2500 3600 6000 5000 7200 6300 3700 840 1100 940 1000 840 660 160 280 160 230 250 270 690 490 470 580 600 570 PC(34:3) 40 46 67 51 96 83 130 170 87 180 110 950 320 180 190 160 380 89 130 150 85 100 95 25 32 48 33 40 20 33 PC(34:2) 380 470 820 620 670 1000 930 1500 1700 1100 2000 1200 1100 3000 4000 2700 2700 2500 1700 3000 3200 2100 2600 2000 1100 1600 2200 1400 1600 1400 220 440 310 290 360 400 780 390 470 300 340 390 PC(34:1) 800 1000 1500 1800 2400 1700 3100 3000 3000 5100 2900 2700 6400 7400 7100 7000 6100 5000 9100 8600 8700 9300 5800 2300 3200 4000 3300 3800 3000 460 890 590 760 870 950 1700 850 930 880 1000 1000 PC(34:0) 290 550 690 1100 850 2700 890 1600 2300 1800 1400 690 630 680 990 1300 1800 1900 2700 3400 2900 4800 3100 1500 170 670 1100 910 470 3800 86 160 110 160 160 170 350 450 450 550 590 570 PC(36:4) 140 170 280 300 320 440 240 470 420 530 680 380 680 950 890 890 780 940 1400 1400 1800 1400 270 380 440 430 350 310 89 110 100 230 160 160 170 180 200 PC(36:3) 290 320 490 390 360 640 690 1200 1200 720 1400 780 2400 1900 2200 1300 1400 1500 1200 2100 2000 1400 1300 730 1000 1400 750 840 740 140 230 200 180 160 210 250 290 320 190 230 250 PC(36:2) 2100 2800 3700 2600 2700 4800 4800 7900 8300 4800 9100 6200 5000 15000 18000 9900 12000 13000 11000 17000 16000 9600 9800 8300 5800 8400 9800 5600 6400 6100 1000 2000 1400 1000 1300 1700 3900 2100 2200 1400 1600 2100 PC(36:1) 1600 2400 3500 6800 6400 6700 11000 5200 3100 7900 11000 11000 13000 11000 9600 13000 12000 16000 12000 8100 3100 5000 4600 5300 5200 4000 520 950 710 1000 1200 1300 1900 1800 1900 2000 2200 2200 PC(36:0) 250 400 830 1300 1100 1500 440 1000 920 1700 1800 610 400 220 840 910 1100 1000 400 1800 350 150 270 210 69 19 35 27 35 230 280 460 480 380 PC(38:6) 950 1400 1900 1900 2100 3400 1800 3600 3500 3300 5700 3300 3900 5000 6800 5200 6800 7300 7400 11000 9600 8500 7600 6600 1800 2700 2800 2400 2600 2700 310 650 510 560 670 910 1100 1000 1200 1000 1300 1600 PC(38:5) 430 650 720 900 760 1200 780 1600 1500 1600 1900 950 3400 1700 2100 1900 2100 3600 4000 3500 5200 3800 2700 630 920 730 710 620 620 150 210 140 180 160 230 410 470 570 530 590 680 PC(38:4) 310 420 560 360 250 670 730 1300 1300 880 1400 670 3100 1100 1700 1400 1100 1200 1500 1300 650 930 580 830 830 440 400 530 91 200 120 110 140 180 230 330 330 240 240 280 PC(38:2) 550 740 860 680 610 1100 1300 2100 1900 1400 2500 1300 2700 2300 3000 1900 2300 2600 2600 2400 2000 1900 920 1500 1400 950 980 1100 190 310 220 210 230 320 510 520 590 390 450 560 PC(38:1) 420 780 620 670 630 760 360 1200 980 1600 1300 500 2300 2200 3400 2500 2300 3100 6000 5600 7100 6100 5700 4400 740 640 960 810 800 890 110 260 120 170 140 160 380 25 130 36 670 77 PC(38:0) 1100 1400 2300 1700 1800 2600 2900 4800 5300 3300 6000 2900 1300 3900 5700 3300 4400 4200 3900 5900 5200 3500 3500 1800 2700 3000 2000 2200 1700 320 560 450 340 490 570 980 86 86 93 22 150 PC(40:7) 1100 340 540 330 460 440 540 830 3800 630 4400 2500 5300 3800 4700 2800 3800 5200 3400 6500 4600 1300 2200 2000 1500 1600 1800 250 510 340 370 380 590 910 1100 720 930 1400 PC(40:6) 1100 320 430 460 550 730 420 430 810 990 350 5200 3600 3900 4900 5000 2900 850 700 1700 1100 1800 1600 1800 1800 1800 200 380 290 420 460 570 610 930 1100 1100 1400 1700 PC(40:4) 200 360 470 520 970 460 3300 780 720 750 200 330 360 320 230 410 170 160 190 170 200 240 PC(40:2) 89 93 180 170 94 210 200 340 400 330 430 200 300 280 220 180 170 180 170 42 93 100 100 110 140 PC(40:1) 310 410 560 440 410 660 760 1300 1300 910 1400 630 980 1300 830 950 1200 1400 1300 450 640 510 330 430 340 92 110 110 68 96 86 180 220 290 180 210 260 PC(42:2) 590 730 860 610 510 880 1300 2400 2000 1600 1900 910 1200 1600 1300 1300 1700 2000 1500 1800 600 950 900 520 550 520 240 170 150 110 150 310 420 510 310 340 440 PC(42:1) 640 740 750 700 630 990 1300 2300 2000 1700 2000 1000 1200 2000 1300 1700 1700 2100 1700 1300 3300 590 910 940 610 700 650 170 300 170 120 130 210 230 370 510 310 430 500 PE(32:0) 130 160 120 150 150 200 430 870 680 1000 1400 520 570 730 670 790 670 730 750 770 810 800 1000 820 110 170 190 150 140 81 22 30 21 150 130 180 170 180 190 PE(34:2) 560 610 620 600 560 910 1100 2200 1600 2100 3300 1800 3000 4300 5600 4400 4000 4600 2900 3400 4000 3100 4500 6100 800 1200 1900 1100 1700 1400 90 170 160 150 240 270 700 470 790 580 670 850 PE(34:1) 1000 1100 940 1400 1200 1700 2500 4600 3800 5500 8300 3900 5700 6700 7600 8300 7200 7800 5500 6500 7200 8000 10000 12000 1300 1600 2400 1800 2400 2000 150 250 220 310 410 400 1300 930 1400 1200 1500 1700 PE(34:0) 240 260 170 230 210 290 660 1300 930 1500 1800 770 510 450 800 470 710 790 890 1000 860 970 1000 900 110 180 95 180 81 200 180 260 260 290 390 PE(36:4) 430 440 500 470 460 530 330 1700 640 920 950 2600 3100 3800 3100 2600 2500 2500 2900 3400 3200 3500 3600 650 760 1200 750 1100 1000 120 200 150 130 190 190 570 390 590 240 240 560 PE(36:3) 410 480 470 410 440 710 1100 1700 1500 1700 2600 1300 2500 3100 4100 3300 3200 3500 2200 2900 3100 2500 3200 4300 710 930 1400 850 1200 1100 85 160 140 180 210 590 410 600 500 580 610 PE(36:2) 1900 2000 2000 1800 1500 3000 3300 6400 4800 5200 7400 5300 10000 14000 16000 11000 10000 14000 9100 12000 13000 9600 12000 18000 3100 4200 6200 3300 4700 4900 470 900 770 460 920 1200 2400 1600 2300 1400 1800 2600 PE(36:1) 1400 1600 1400 1700 1600 3000 3600 7100 5000 6700 11000 5900 6900 7400 8500 9500 8900 12000 6700 8500 9400 11000 13000 17000 1700 2100 2700 2400 3400 3200 210 340 310 420 580 780 1900 1300 1700 2000 2900 PE(36:0) 420 520 520 670 710 890 1300 2500 1800 2800 4000 1700 2000 2700 2700 2800 2500 3400 2900 3400 4100 3800 4500 4400 580 810 920 770 940 780 92 160 120 120 190 190 570 480 820 590 670 980 PE(38:6) 200 200 180 210 190 210 200 970 690 960 1300 670 1100 950 1300 1400 820 230 740 1400 1300 1200 1000 1500 260 320 420 300 310 270 58 55 79 260 210 91 75 260 220 PE(38:5) 660 810 750 580 490 930 2100 3700 2800 2900 4500 2600 2700 3800 5000 2800 2800 4000 3600 4400 4400 3200 3900 4000 940 1300 1700 800 1100 1200 100 180 180 130 200 260 1100 760 1100 620 720 1000 PE(38:4) 1300 1400 1400 1200 1200 1800 3900 6600 5100 5600 8200 4500 4500 6500 7900 5700 5500 7800 6000 7300 8100 6200 7500 8000 1600 2200 2600 1700 2200 2100 190 370 350 220 380 490 1800 1300 1800 1200 1400 1700 PE(38:3) 980 1100 1000 910 870 1400 2900 4800 3900 4600 6600 2800 3500 3900 4700 3900 3700 4800 4600 5500 5600 4500 5200 5600 1100 1300 1600 1000 1500 1300 120 220 210 180 220 330 1200 880 1300 910 1000 1300 PE(38:2) 390 440 440 420 360 670 890 1600 1400 1700 2000 1000 1400 1300 2100 1500 1900 2900 1700 2200 2200 1800 1800 3000 460 590 690 480 590 730 54 80 77 67 100 150 430 310 490 340 420 680 PE(38:1) 280 270 320 280 250 530 650 1200 920 1100 1700 960 1300 1700 1900 1300 1100 2000 1700 2000 2200 1700 1800 370 400 570 370 390 430 43 90 72 100 110 370 300 400 230 280 480 PE(38:0) 440 660 590 650 500 980 1100 2200 1700 2100 2900 1600 2400 3700 4100 3200 2800 3600 3500 4800 4600 3900 4700 5200 750 990 1100 800 940 1000 91 200 150 130 170 210 660 580 770 580 680 810 PE(40:6) 410 480 440 410 420 700 1400 2700 2100 2400 3700 1800 1300 1800 1900 1700 1700 2700 1900 2200 2100 1900 2300 2500 450 610 750 540 710 760 140 540 430 610 440 570 640 PE(40:5) 400 460 450 530 540 910 1300 2500 1700 2700 4100 1900 1300 1500 1800 1700 1900 2800 1700 2200 1800 2100 2500 2800 390 530 560 510 780 770 61 110 93 100 130 220 480 360 530 480 630 770 PE(40:4) 260 260 240 380 310 730 740 1400 1100 1600 2100 1100 730 900 940 980 1200 2000 1100 1200 1400 1300 1400 2300 240 320 410 350 440 630 37 60 64 110 140 260 210 330 310 320 540 PS(34:2) 28 32 64 62 53 86 77 130 110 200 120 82 300 370 370 240 240 350 500 610 480 490 65 82 93 65 27 43 27 34 28 PS(34:1) 180 210 270 270 270 310 250 530 460 810 770 300 3400 2000 2200 2400 1600 1500 2600 3700 4800 4200 4200 3400 530 440 670 480 470 390 56 77 88 88 95 100 300 200 280 200 250 250 PS(34:0) 28 33 44 41 35 51 36 77 70 100 100 27 210 120 180 150 120 130 310 410 460 480 320 260 19 39 17 19 31 38 33 41 26 PS(36:4) 34 41 59 59 47 81 56 120 97 140 160 60 340 210 200 230 160 130 270 490 530 450 360 68 61 110 62 91 55 66 30 40 31 37 34 PS(36:2) 180 200 260 190 190 270 250 520 420 560 600 270 2700 1800 1900 1600 1000 1300 2300 3500 4200 2700 2700 2700 440 410 620 300 320 310 53 72 78 43 44 87 310 180 260 150 190 230 PS(36:1) 850 1000 1100 1200 1100 1600 1200 2800 2000 3400 3100 1600 15000 8200 8700 8600 5700 7700 12000 19000 20000 18000 15000 18000 2200 1900 2400 1700 1600 1700 310 400 350 370 390 590 1300 920 1300 890 1100 1300 PS(36:0) 630 810 1100 1400 1400 1900 890 1900 1800 2100 2100 990 620 210 160 570 210 3800 5400 4800 4700 810 600 1200 1200 1300 1200 1300 1500 510 710 650 740 660 1400 280 190 190 280 580 680 PS(38:5) 65 80 110 62 99 140 91 200 150 190 210 83 450 360 360 310 210 240 500 730 800 610 470 360 110 100 140 100 130 110 48 81 44 78 49 51 91 PS(38:4) 200 230 280 250 250 320 260 580 550 690 730 270 2400 1400 1500 1400 880 1200 2200 3400 3500 2600 2400 2300 330 340 500 260 280 240 46 74 63 60 51 68 310 180 290 190 230 300 PS(38:3) 120 140 160 150 130 170 160 370 310 470 450 140 1600 980 1200 880 660 670 1900 2300 2700 1900 1500 1500 210 210 300 160 170 140 35 40 39 41 37 65 160 130 190 110 130 150 PS(38:2) 64 81 71 76 71 100 78 210 140 220 200 92 700 540 550 520 320 410 920 1300 1200 880 980 930 120 110 120 79 80 92 26 89 63 97 70 81 73 PS(38:1) 44 51 60 66 70 120 76 170 120 230 260 81 730 380 330 450 280 400 640 990 960 920 670 940 97 72 110 73 91 77 61 50 64 54 71 77 PS(40:6) 170 160 220 180 180 210 200 460 380 560 520 200 1900 1300 1500 1100 740 940 2200 2900 3700 2100 1800 1900 300 300 490 200 210 200 47 50 61 51 53 69 220 170 280 150 180 200 PS(40:5) 120 140 150 120 130 160 160 390 270 430 380 150 1600 970 1200 900 620 720 2000 2300 2600 1600 1600 1700 240 230 330 160 170 130 29 40 34 36 37 33 170 120 200 100 150 150 PS(40:4) 57 57 73 76 72 130 82 170 140 210 230 81 790 540 580 460 310 410 800 1100 1200 960 880 990 120 110 140 74 100 97 16 22 20 77 60 86 60 70 89 PS(40:3) 46 51 55 65 62 100 57 140 120 180 220 72 560 400 330 550 320 350 670 860 730 830 790 940 80 84 110 77 84 75 12 64 43 59 56 76 79 PS(40:2) 26 24 27 42 30 37 31 78 57 90 77 40 400 180 220 120 130 140 380 420 440 390 240 260 52 38 24 25 30 20 28 32 PS(40:1) 27 20 41 40 75 65 110 99 320 140 170 220 85 130 290 380 370 370 320 320 50 28 21 35 29 24 29 PS(40:0) 37 55 58 41 48 65 57 130 80 140 140 72 270 220 220 190 150 210 270 540 320 260 250 280 42 87 91 60 70 50 35 48 33 30 45 PS(42:4) 58 50 110 110 110 160 85 180 160 180 190 100 160 130 130 120 160 210 310 220 340 250 210 73 93 110 100 110 120 42 61 54 60 50 81 28 42 44 47 50 PS(42:3) 33 49 56 71 73 90 53 120 99 120 120 66 170 150 200 130 190 210 270 320 320 260 310 91 94 110 110 130 160 47 73 46 63 60 130 27 43 38 39 45 PS(42:2) 19 28 32 48 37 68 46 110 50 100 92 55 80 46 82 89 110 93 240 120 210 280 9.3 20 44 4.2 25 13 36 PS(42:1) 25 27 36 38 66 38 82 71 110 110 54 190 64 140 120 110 130 200 160 410 180 38 74 36 26 32 44 39 PS(42:0) 34 40 75 51 30 57 80 100 150 130 140 12 14 12 18 PI(34:2) 21 33 28 31 26 26 45 54 39 PI(34:1) 6.3 7 13 24 18 22 16 45 31 100 74 21 69 53 67 100 85 42 46 85 100 160 100 14 26 6.7 49 6.4 6.3 11 9.6 8 PI(36:5) 8.5 12 21 22 22 31 12 24 23 22 30 17 18 24 19 21 23 28 24 46 35 42 31 28 17 21 23 17 28 31 9.5 16 11 13 12 23 5.7 4.9 6.3 5.6 6.8 8.7 PI(36:4) 3.5 3.6 7.9 10 6.9 11 8.5 18 15 26 19 7.8 23 27 39 24 19 17 15 40 34 28 29 40 11 11 12 9.6 11 6.3 2.8 6 4 3.9 4.2 4.2 17 2.6 3.5 2.4 3.1 3.2 PI(36:3) 2.8 16 15 7.3 10 15 11 24 63 16 21 7.3 100 22 84 82 120 72 73 17 48 31 100 20 11 18 17 11 2.8 7 10 8.9 11 PI(36:2) 17 24 36 42 31 49 62 140 110 230 160 66 210 240 290 230 220 180 160 240 310 290 230 170 48 65 70 37 36 33 5.2 7.7 9.2 2.9 9.9 8.2 20 19 22 18 21 21 PI(36:1) 12 14 18 41 29 31 30 81 63 200 120 30 110 120 130 200 150 89 130 210 200 360 260 96 30 44 52 31 42 19 10 6.8 10 28 12 14 15 19 18 11 PI(36:0) 2 3 3.4 7.7 6.1 10 4.1 12 9.5 29 25 5.3 13 11 7.2 15 16 14 8.9 16 24 35 19 14 1.3 2 5.2 6.4 14 1.9 1.6 2.9 4.2 3.3 PI(38:6) 2.1 4.4 4.5 3.9 1.8 6.5 6.5 7.7 4.9 9.7 11 10 9.7 11 0.95 1.9 0.81 PI(38:5) 6.3 8.7 11 10 7.3 16 18 43 39 43 29 19 55 76 90 45 45 65 46 74 85 54 44 14 20 27 6.9 14 4.4 1.8 7.6 7.2 3.7 5.5 7.5 PI(38:4) 27 34 42 24 24 38 79 180 150 130 84 43 260 260 330 120 110 140 220 320 330 160 130 140 63 83 100 29 39 42 8.4 14 11 4.9 7.4 11 21 27 30 14 15 18 PI(38:3) 31 34 43 27 20 41 88 180 150 130 80 45 330 290 320 120 120 150 280 360 410 170 140 150 72 88 98 31 38 35 9 13 12 5.7 8.8 9.8 26 30 31 13 15 18 PI(38:2) 8.2 7.6 11 10 12 12 19 40 39 55 37 14 77 55 74 49 52 44 53 79 85 96 59 49 14 18 29 12 9 11 1.1 3 7.3 6 8.3 5.3 6.6 5 PI(38:1) 6.2 4.9 2.1 0.48 2.2 13 9.4 PI(40:7) 5.2 4.5 3.7 6.7 3.3 8.6 7.5 8.4 10 4.6 2.6 2.6 2 1.1 1.9 PI(40:6) 3.3 4.1 9.1 14 8 21 7.3 7.5 9.5 13 8.9 5.1 22 25 16 15 13 11 16 17 20 10 8 7.1 7.6 5.7 6.3 2.7 4.6 3.7 3.5 5.8 2 1.2 3.1 1.3 2.7 PI(40:5) 2 2.8 7 3.6 11 11 6.8 12 7.9 27 12 6.1 21 22 22 21 26 28 21 27 24 39 32 5.4 6.7 4.7 1.9 3.1 3 1.9 2.6 2.7 PI(40:4) 3.2 2.2 3.7 12 4.4 9.4 12 17 10 5.7 18 19 29 19 21 21 15 22 20 26 21 4.1 4.9 8.4 4.7 4.6 1.8 1.8 2.3 2.3 2.3 PI(40:3) 3.4 7.9 9.5 10 2.7 6.5 6.5 13 8.9 4.8 11 19 19 22 15 15 18 20 8.5 7.9 8.4 8.7 1.6 2.3 2.1 2.6 2.2 PG(32:1) 47 37 38 100 82 150 69 70 88 160 130 130 95 120 110 200 110 93 150 280 300 210 290 250 33 44 52 53 150 120 25 16 22 42 48 74 PG(32:0) 27 23 69 60 62 22 72 69 270 140 65 92 92 71 160 190 150 92 170 130 320 550 250 26 28 31 48 110 48 7.1 29 16 10 14 40 46 49 PG(34:3) 54 57 58 35 33 55 62 120 100 54 84 82 23 20 25 22 6.5 11 6.9 7.1 10 PG(34:2) 100 120 220 170 170 210 200 520 390 490 600 270 520 570 500 600 380 520 580 990 1100 730 940 830 190 220 370 240 320 340 34 51 50 42 53 75 140 74 100 72 96 120 PG(34:1) 110 180 330 540 440 420 670 670 640 1200 1400 420 750 880 800 2500 940 840 1300 1200 1600 1900 3100 1100 320 370 620 970 390 740 68 100 100 87 89 180 190 210 170 180 230 390 PG(34:0) 30 47 84 210 150 120 51 210 180 490 790 95 190 310 160 270 220 160 330 360 340 630 730 190 110 100 78 180 63 9.5 11 9.4 30 33 14 37 24 47 65 71 88 PG(36:4) 51 88 110 110 210 210 330 250 260 250 340 470 270 370 79 140 120 75 38 34 42 61 PG(36:3) 320 460 720 490 450 710 760 1400 1400 1500 1700 880 1300 1700 1500 1600 1300 1900 1700 2800 3600 2000 2200 2500 660 700 970 650 600 950 91 150 150 99 120 220 480 210 320 200 250 380 PG(36:2) 1100 1700 2500 2000 1900 3800 2600 5500 4600 5600 7400 4100 5000 6400 5500 6200 4900 6800 5900 10000 12000 8200 8500 10000 2000 2200 2600 2200 2300 3100 260 410 370 270 320 690 1400 780 1100 880 1200 1600 PG(36:1) 470 840 1000 1800 1100 1500 1300 3200 2500 5000 5300 1900 2300 2600 2700 4000 3000 4000 3100 4600 5700 4900 2900 3400 610 720 490 510 540 450 62 83 100 94 100 89 440 650 690 810 540 PG(38:5) 130 190 280 310 260 420 310 690 560 860 940 650 470 660 590 690 510 1200 820 1200 1600 920 940 1200 160 190 240 190 250 31 28 75 130 90 160 120 150 190 PG(38:4) 370 620 760 780 690 1300 930 1900 1600 2800 3100 1500 1400 1700 1800 2200 1700 3200 2400 3600 4500 2700 2600 3300 440 520 590 570 550 770 64 110 90 80 97 170 410 260 430 380 480 690 PG(38:3) 820 1200 1300 920 790 1500 2000 4000 3000 3500 4200 1900 2600 3100 3400 2600 2100 4300 4900 6800 8400 2900 3000 4200 850 910 990 590 660 900 120 160 160 84 120 230 660 510 860 450 640 790 PG(38:2) 780 1300 1200 1200 970 1700 1900 4500 2900 5500 5700 2000 2600 3000 3300 3400 2700 4300 4800 6200 7700 3800 3900 4100 840 900 950 880 910 1000 99 150 130 130 130 210 560 530 880 620 800 1000 PG(38:1) 110 150 150 230 240 240 230 730 420 1300 1100 270 480 420 400 700 550 670 350 490 1800 820 840 140 120 140 170 97 81 18 29 27 20 140 130 150 170 130 PG(40:8) 22 53 67 150 130 230 200 88 130 130 190 220 310 34 23 35 43 PG(40:7) 210 360 460 310 280 530 590 1500 1100 1200 1400 660 740 1100 1300 720 620 1300 1500 2200 2700 1000 1000 1300 280 360 400 240 250 350 70 57 52 94 210 190 330 150 210 320 PG(40:6) 180 270 320 290 260 450 490 1200 710 1200 1300 550 560 790 870 690 600 1300 1100 1900 1900 840 980 1300 210 220 230 200 200 330 39 37 38 46 74 180 150 230 130 190 320 PG(40:5) 140 220 200 190 170 320 360 710 470 730 840 410 390 580 560 530 390 800 960 1100 1400 500 520 890 140 170 170 130 130 190 27 25 27 17 23 43 72 99 170 87 130 190 PG(40:4) 140 190 170 170 150 240 330 720 420 790 790 380 430 510 560 570 460 810 920 930 1200 520 620 690 130 150 150 120 150 130 13 32 24 42 89 150 94 120 180 PG(40:3) 120 160 140 120 120 210 290 630 380 660 630 270 420 480 470 410 320 700 760 920 1100 370 490 550 100 130 120 110 17 20 32 80 140 69 88 190 PG(40:2) 54 81 88 99 71 100 110 250 170 400 320 130 130 220 240 350 240 270 320 330 470 300 200 260 51 59 60 39 61 49 48 59 CL(66:5) 19 6.4 8.3 5.9 7.9 3.6 CL(66:4) 11 11 2.8 3.6 2.6 4.1 150 56 62 48 64 35 9.3 34 14 7.2 6.5 8.5 22 14 22 13 8.8 9.9 CL(66:3) 5.1 3.8 0.69 2.2 1.8 1.5 110 35 33 53 69 29 1.8 15 6.5 5 3 11 11 5 8.6 7.1 8.2 6.2 CL(66:2) 1.5 0.88 0.72 1.1 1 0.31 4.9 4.8 3.6 0.18 6.4 4.4 2.9 CL(68:5) 11 12 3.4 4.4 2.9 4.4 210 74 79 63 83 45 17 45 20 11 5.9 11 48 19 6.3 7.7 7.5 7.7 4.7 8.1 4.3 4.6 3.2 4.5 29 18 25 14 12 17 CL(68:4) 53 57 11 20 12 18 710 180 150 240 280 120 78 190 66 44 26 42 71 23 15 22 7.5 20 4 11 7.9 6.3 4.8 6.2 140 72 120 69 55 75 CL(68:3) 0.41 3 2.9 8.3 10 0.36 83 120 30 16 11 3.2 19 4.7 1.2 8.5 7.2 2.9 5.1 7.1 2.9 4.8 3.2 4.1 20 22 CL(68:2) 55 9.5 7.2 52 60 4.4 CL(70:6) 9 9.8 2.4 3.5 2.2 3.5 160 58 61 48 68 36 16 46 14 9.2 5.5 11 26 12 24 11 8.6 12 CL(70:5) 43 46 8 15 9.2 17 720 190 170 220 280 120 68 160 61 38 19 45 59 21 15 18 8.4 22 6.1 16 7.7 8.7 4.6 7.1 120 64 95 50 47 68 CL(70:4) 69 70 12 24 16 21 840 180 150 250 300 110 110 250 75 50 38 57 73 27 15 22 9.9 21 3.7 14 8.5 10 5.2 5 210 99 130 87 75 90 CL(70:3) 50 78 8.9 2.7 0.28 3.3 1.8 3.4 2.6 4.2 5.4 0.9 2.5 1.9 2 CL(72:7) 8.1 8.9 1.8 2.7 1.8 3.1 110 40 38 33 48 23 14 33 8.4 5.1 4.2 6.8 20 11 17 8.2 9.1 10 CL(72:6) 16 18 2.9 5.7 3.5 6.3 280 95 85 85 120 62 22 66 20 15 6.4 16 45 24 34 18 19 24 CL(72:5) 29 29 4.8 9.5 6 11 520 130 110 150 200 79 54 120 37 19 14 26 89 43 60 35 36 45 CL(72:4) 23 27 5.6 2 400 86 67 100 130 43 38 91 19 16 9.8 17 71 32 40 24 26 27 PC(P-36:4)/PC(O-36:5) 460 690 600 1100 920 1300 1000 2000 1700 2500 3600 1300 2100 1900 2000 2700 2500 2500 2400 4100 2900 6100 5000 3800 570 830 700 830 910 760 130 190 120 200 240 240 370 420 410 600 650 720 PC(P-36:3)/PC(O-36:4) 3.5 76 19 48 72 80 1500 170 150 86 84 160 280 480 52 63 130 64 79 73 39 33 47 36 7.6 PC(P-36:2)/PC(O-36:3) 110 120 200 180 150 250 230 330 390 280 500 270 1400 550 750 840 610 550 380 1100 990 590 1600 4600 200 290 270 260 260 240 51 64 75 120 76 100 76 91 130 PC(P-36:1)/PC(O-36:2) 310 49 550 430 350 780 700 1300 1200 840 1600 1000 1700 2000 2700 1600 2000 2500 180 2400 2000 1400 2300 1900 660 1100 1100 730 860 970 160 240 160 190 180 330 360 320 370 250 290 430 PC(P-38:4)/PC(O-38:5) 180 240 320 280 490 380 680 650 430 720 550 2500 980 980 830 1200 2000 1300 2300 5900 250 480 500 250 340 450 150 210 140 170 240 PE(O-32:0) 720 890 1300 1400 1300 1800 850 1800 1400 1900 2100 1400 1200 2300 1800 2400 1700 2600 2500 4100 3700 4300 3500 4000 1100 1300 1300 1200 1300 1400 370 540 590 490 620 880 280 530 610 540 610 660 PE(P-36:4)/PE(O-36:5) 510 580 590 590 600 720 730 1400 1100 1300 1600 1000 4900 6100 6300 6000 3600 3500 3600 5700 6100 4900 5500 7700 1300 1500 2600 1500 2100 1700 260 490 290 370 370 380 830 560 700 510 540 610 PE(P-36:3)/PE(O-36:4) 460 590 680 690 650 750 640 1300 950 1300 1900 930 4700 5000 5500 6100 4000 3600 3600 4900 5400 5100 5500 7000 1200 1300 2100 1700 1900 1500 190 320 250 290 330 300 820 510 670 600 640 580 PE(P-36:2)/PE(O-36:3) 450 540 620 560 540 780 540 1000 830 1000 1300 770 4300 5200 6000 5200 3500 3800 3300 4500 5300 4500 4900 7300 1100 1400 2100 1300 1700 1500 160 360 260 250 290 320 720 470 690 500 550 570 PE(P-36:1)/PE(O-36:2) 620 710 720 1000 870 1500 510 1100 920 1400 2100 1300 6900 7300 7100 9000 6400 7100 4600 6600 7200 8000 9600 16000 1400 1800 2600 2100 2800 2700 220 470 290 420 500 590 940 530 910 810 830 1100 PE(P-36:0)/PE(O-36:1) 230 210 270 510 490 790 180 390 260 840 1000 740 1400 1500 1900 2600 1500 2900 1100 2200 2000 3200 3600 5100 410 390 400 630 750 690 27 30 57 87 150 180 300 210 350 330 340 540 PE(P-38:4)/PE(O-38:5) 930 1200 1300 1200 1000 1600 2000 3800 2700 3200 4900 2700 8000 11000 11000 8600 6700 7500 6700 11000 11000 9300 10000 13000 2200 2700 3300 2300 2600 2300 250 540 380 360 450 480 1700 1200 1500 1100 1200 1400 PE(P-38:2)/PE(O-38:3) 530 660 1200 1300 1300 1900 1100 2400 1700 2300 2800 1800 4300 4800 5600 4600 4500 5900 4900 6500 6800 6600 6500 8100 1500 1700 1800 1600 1700 1800 380 580 520 540 590 920 900 600 1000 630 980 920 PE(P-38:1)/PE(O-38:2) 410 480 390 470 410 670 450 890 800 1100 1600 1000 3900 3600 3600 3600 3000 3900 3000 3900 4200 3800 5000 6100 700 720 800 680 680 700 46 100 58 60 120 68 580 450 570 510 570 810 PE(P-38:0)/PE(O-38:1) 140 170 120 300 230 540 200 430 260 690 1000 630 530 510 620 1100 1100 2000 840 1200 990 1600 1600 2400 160 130 150 210 230 270 6.9 15 47 56 91 100 120 200 250 320 470 Coenzyme Q9 2000 1700 350 900 440 1200 17000 14000 12000 14000 19000 6900 3000 1600 3200 1400 1700 1600 570 300 360 220 120 350 130 140 140 120 110 140 32 24 18 20 27 49 1800 1500 1800 1700 1600 1800 Coenzyme Q10 190 170 39 110 53 130 1500 1200 930 1400 2000 640 340 170 330 180 220 180 86 40 48 33 20 43 18 14 14 14 13 13 4.4 2.4 1.6 2.4 2.9 4.9 170 140 160 190 190 180 Dolichol-18 5.8 3.4 3.5 8.2 4.4 9.4 7.5 6.4 4.3 6.3 7.2 3.4 6.8 14 9.9 8.1 11 6.7 5.8 3.1 3.4 2.7 2.5 3 1.6 2.9 2.8 5.7 2.6 1.5 0.25 0.12 0.11 0.14 0.16 0.21 2.6 2.6 2.3 3.7 3.9 3.6 Dolichol-19 3.5 2.1 1.7 4.2 3.1 5.8 5.2 4.2 3 4.2 5 2 10 4.7 6.4 6.1 8.5 4.9 3.3 2.1 2.4 2 1.4 1.9 2.1 1.9 1.4 1.8 1.5 0.94 0.18 0.095 0.067 0.079 0.093 0.13 2 1.5 1.6 2.1 2.2 2.3 Dolichol-20 0.62 0.43 0.39 0.88 0.6 1 1.1 1 0.64 1 1.2 0.51 1.1 2.1 1.4 1.4 2 0.98 0.4 0.42 0.75 0.31 0.37 0.42 0.2 0.35 0.33 0.72 0.36 0.17 0.033 0.02 0.015 0.016 0.017 0.028 0.38 0.35 0.35 0.46 0.51 0.46 Cer(d18:1/16:0) 110 100 86 180 120 220 67 83 73 210 180 230 230 190 170 300 250 320 260 310 250 290 560 41 33 38 43 57 63 8.9 8.3 12 17 11 27 83 86 87 170 170 180 Cer(d18:1/18:0) 5.7 12 11 25 8.6 23 7.1 8.4 11 22 10 25 8.9 12 23 31 20 37 24 25 22 40 18 6.8 9.5 12 16 5.1 15 2.9 1.9 3.8 4.6 3.3 7.8 5.2 9.3 7.4 19 6.1 18 Cer(d18:1/20:0) 2.3 3.1 2 10 2.6 3.8 3.3 4.1 4.4 9.6 5.7 6.5 6.5 8.3 8.6 9.1 7.6 7.3 12 8.4 9.5 14 8.1 5.5 6.3 6.8 3.2 4.8 4.7 2.6 1.7 1.7 4.4 1.2 3.4 1.5 2.5 2.1 9.7 3.4 3.8 Cer(d18:1/22:0) 12 9.9 7 20 15 32 5.3 10 6.3 28 13 16 21 14 13 38 22 26 32 36 23 35 41 5 2 3.5 6.4 4.1 5.4 2.8 0.59 0.25 4.4 1.7 2.2 8.9 9.9 6.4 24 19 22 Cer(d18:1/24:1) 34 23 21 28 43 41 20 28 16 52 46 37 60 42 41 64 62 62 58 72 61 71 97 15 21 12 14 22 11 7.9 4.5 4 5.5 3.3 3.3 30 21 17 49 39 40 Cer(d18:1/24:0) 23 17 9.9 43 33 56 19 14 14 45 36 37 38 21 30 57 55 42 72 54 45 100 120 12 10 5.2 17 12 7.4 4.4 2.9 1.7 5 3.7 8.5 29 18 16 45 52 53 Cer(d18:1/26:1) 1.3 1.2 0.68 4.1 1.6 3.2 0.87 1.2 3.1 1.1 2 1.3 1.8 4.6 2 1.2 0.26 1.7 1.2 3.8 0.98 2.8 1.3 3.6 0.84 1.8 2.3 0.66 0.97 0.32 1.4 1.1 1.4 0.45 1.1 0.55 1.1 0.65 Cer(d18:1/26:0) 1.1 3.2 3.1 9.4 3.5 2 4 6.4 1.7 4.4 4.9 4.9 3.3 6.4 4.9 4.7 6.6 5.9 7.5 9.4 6.8 11 12 6.3 8.8 4.5 7.1 7.9 3.3 1.4 2 1.2 3.1 1.1 1.1 3.3 1.5 1.4 4.3 0.87 1.5 Cer(d18:0/16:0) 3.2 2.6 2.3 15 4.6 5 2.6 1.9 3.9 12 6.9 8.3 6.7 3.2 6.8 13 12 12 11 12 13 12 21 1.7 1 1.6 2.6 2.1 2.4 1.3 0.35 0.92 1.4 0.95 1.3 2.3 3.4 4.2 9.6 7 7.4 Cer(d18:0/18:0) 0.49 0.27 1.2 4.3 0.91 2.8 0.88 1.2 0.44 3.4 2.4 0.92 2 1.2 3 3.1 3.1 2 1.5 3.4 3.2 2.3 3.5 0.76 0.39 1 1.6 1.6 0.4 0.49 0.11 0.51 0.18 0.13 0.43 0.94 0.7 1.3 3 0.29 1.4 Cer(d18:0/20:0) 0.41 1.1 0.98 0.18 1.6 0.62 0.48 0.38 0.82 1.2 2.1 0.9 0.3 0.68 1.5 1.6 0.27 1.7 0.3 1 2.1 1.7 0.89 0.84 0.31 0.86 1 0.43 0.39 0.59 0.37 1.3 0.67 0.51 Cer(d18:0/22:0) 0.71 0.44 0.46 1.8 1.2 1 0.7 0.95 0.99 2 1.1 0.27 1.2 1.2 2.3 2.8 2.7 2.2 1.3 2.7 4.4 6 0.32 0.56 0.88 0.63 0.67 0.73 0.11 0.12 0.47 1.1 0.27 0.85 1.2 0.75 1.5 Cer(d18:0/24:1) 1 2 0.97 2 1.1 2.3 1.2 1.4 1.6 2.1 2.7 1.4 1.4 1.1 1.2 3.2 1.7 1.3 2.3 1 3.5 2.8 2.9 0.23 1.5 0.54 1.5 0.81 0.6 0.35 0.41 0.18 0.54 0.43 1.1 0.67 0.72 2.5 0.75 1.2 Cer(d18:0/24:0) 0.84 0.93 1.4 2.3 1.1 2.5 1.5 0.88 1.1 1.9 2.7 1.4 1.6 1.4 2.2 2.2 2.6 2.9 2.1 3.4 1.5 2 4.5 0.74 0.32 0.57 0.55 1.1 0.82 0.5 0.12 0.18 0.29 1 0.52 1.3 1.9 2 2.1 Cer(d18:0/26:1) 0.17 0.23 0.18 0.19 0.13 0.43 1.2 2.3 0.91 1.8 0.63 0.68 0.61 2.1 1.4 1.2 2.2 1.3 0.99 0.39 0.95 0.63 0.12 0.6 0.27 0.18 0.49 1.3 0.14 0.077 0.24 0.1 0.095 0.16 Cer(d18:0/26:0) 0.31 0.43 1.1 1.5 1.1 1.7 0.46 2.4 1.3 0.61 0.85 0.53 1.8 1.3 3.1 0.94 1.7 1.3 1 2.1 0.9 1.1 2.8 0.72 0.84 0.35 0.89 0.51 0.23 0.11 0.29 0.28 0.66 0.68 0.82 0.5 0.095 0.28 HexCer(d18:1/16:0) 44 18 11 27 32 21 62 53 22 94 100 60 340 140 100 230 200 120 320 340 160 240 380 33 32 15 31 26 19 9 5.4 5.2 7.1 6.2 6.8 74 51 28 89 82 40 HexCer(d18:1/18:0) 2.5 1.7 0.24 2.5 1.1 1.6 3.5 5.1 0.56 8.2 4.3 3.7 15 7.4 4.1 18 9.7 6.2 17 14 7.3 17 12 1.6 1.7 0.45 2.4 1.1 0.28 0.91 0.21 0.075 0.51 0.93 0.72 4.3 3.3 1.6 5.8 2.7 2.4 HexCer(d18:1/20:0) 2 0.97 0.5 2 0.88 0.75 3 3 0.36 5.3 4.1 2.2 15 7.5 3.5 14 5.8 4.4 14 15 5.4 16 10 2.5 1.8 0.41 2.3 2.3 0.62 0.56 0.63 0.24 0.46 3.4 1.9 0.75 6.1 2.5 1.7 HexCer(d18:1/22:0) 32 6.3 1.4 18 9.2 3.6 46 17 3.4 74 38 11 260 43 27 170 63 23 210 120 42 160 98 22 7.5 1.8 19 6.5 2.1 4.8 1.3 0.4 3 1.3 0.86 59 19 4.1 64 25 12 HexCer(d18:1/24:1) 34 4.8 0.76 14 8 1.7 48 19 1.8 55 25 4.8 230 35 19 120 40 9.5 180 81 27 100 71 22 4.2 1.9 14 4.4 0.94 4 0.44 0.4 1.8 1.3 0.36 51 12 2.2 40 17 7.8 HexCer(d18:1/24:0) 71 6.9 0.52 48 20 4 100 23 3.9 130 40 9.6 520 60 29 290 92 22 410 120 46 280 160 48 8.8 2.6 34 11 2.3 8.2 0.96 0.4 4.7 1.4 0.17 120 21 3.5 110 41 15 HexCer(d18:1/26:1) 0.69 0.23 0.07 0.24 0.4 0.091 1.1 0.27 0.063 0.67 1.5 0.036 4.1 1.8 0.098 2.2 0.56 2.4 0.59 0.56 1.6 0.9 0.32 0.17 0.76 0.12 0.029 0.14 0.98 0.28 0.045 1.1 0.12 0.12 HexCer(d18:1/26:0) 0.59 0.22 0.033 0.9 0.29 0.089 0.77 0.13 1.7 0.53 0.2 3.6 0.74 0.3 2.9 0.45 0.11 3.6 0.09 3.5 1.2 0.57 0.16 0.045 0.64 0.2 0.062 0.086 0.017 0.15 0.096 0.95 0.26 0.031 1.2 0.36 0.05 HexCer(d18:0/16:0) 0.76 0.38 0.18 0.35 0.43 0.68 1 1.1 0.94 1.1 1.3 1.3 4.3 2.4 2.5 3.6 3.4 2.7 4.5 6 3.2 2.5 5.2 0.48 0.4 0.3 0.48 0.68 0.59 0.21 0.12 0.099 0.04 0.087 0.2 1.2 0.88 1.1 1.1 1.6 0.88 HexCer(d18:0/18:0) 0.056 0.044 0.078 0.03 0.053 0.14 0.079 0.08 0.035 0.23 0.43 0.14 0.33 0.25 0.051 0.3 0.23 0.25 0.33 0.24 0.064 0.2 0.049 0.071 0.064 0.04 0.077 0.2 0.056 0.076 0.11 HexCer(d18:0/20:0) 0.082 0.023 0.078 0.11 0.046 0.037 0.08 0.08 0.088 0.24 0.054 0.24 0.039 0.051 0.15 0.18 0.25 0.036 0.31 0.074 0.087 0.071 0.061 0.091 0.02 0.039 0.039 0.037 0.037 0.018 0.083 0.047 0.062 HexCer(d18:0/22:0) 0.099 0.034 0.11 0.19 0.1 0.099 0.21 0.13 0.14 0.23 0.74 0.14 0.61 0.56 0.28 0.69 1 0.8 1.4 0.21 1 0.077 0.057 0.039 0.056 0.071 0.079 0.042 0.27 0.098 0.09 0.18 0.2 0.28 HexCer(d18:0/24:1) 0.15 0.059 0.12 0.11 0.38 0.066 0.15 0.13 0.26 0.23 1.4 0.4 0.96 0.56 0.081 0.42 1.1 0.58 1 0.36 0.53 0.16 0.051 0.18 0.036 0.24 0.27 0.11 0.053 0.11 0.23 0.13 HexCer(d18:0/24:0) 0.14 0.023 0.045 0.096 0.099 0.11 0.23 0.26 0.15 0.18 1.8 0.3 0.63 0.63 0.4 0.52 0.53 0.34 1 0.59 0.83 0.3 0.057 0.051 0.11 0.056 0.14 0.022 0.022 0.43 0.03 0.036 0.39 0.32 0.42 HexCer(d18:0/26:1) 0.023 0.02 0.056 0.038 0.051 0.087 0.055 0.11 0.061 0.064 0.022 0.027 0.014 0.013 0.011 HexCer(d18:0/26:0) 0.013 0.03 0.082 0.089 0.098 0.058 0.057 0.063 0.029 0.053 0.022 0.24 0.011 0.072 0.027 SM(d18:1/16:0) 2300 810 520 1100 980 1000 2200 1900 1300 3000 2200 1600 16000 8600 8200 13000 7300 4300 23000 18000 14000 20000 17000 1700 1100 790 1600 950 540 500 210 130 300 260 240 5700 3300 2400 5000 4100 2100 SM(d18:1/18:0) 100 36 29 58 26 21 94 73 46 120 67 38 650 300 250 450 180 120 930 580 590 700 400 77 46 19 71 30 25 24 10 7.5 16 7.9 11 240 120 54 180 120 75 SM(d18:1/20:0) 36 9.7 16 21 7.2 9.7 36 23 6.2 38 11 14 240 110 73 150 50 31 340 140 100 250 95 29 14 7.5 20 5.2 11 8 2.7 3.1 4.6 2.9 33 94 29 18 62 32 11 SM(d18:1/22:0) 310 52 19 130 56 38 310 120 20 360 130 32 2000 540 420 1300 370 130 2900 860 600 2100 710 250 81 56 170 58 11 70 14 10 33 16 820 200 85 530 210 100 SM(d18:1/24:1) 1200 180 26 410 160 78 1200 470 140 1200 410 140 8200 2300 1700 4600 1200 390 12000 3800 2300 7600 2600 1000 270 160 640 180 70 290 58 27 110 55 3200 830 220 1800 740 310 SM(d18:1/24:0) 880 120 22 390 160 28 870 250 48 1000 340 92 5700 1200 900 3800 820 260 8300 2000 1400 6300 1800 770 160 76 520 140 64 200 38 18 94 34 2400 430 100 1600 540 230 SM(d18:1/26:1) 14 1.1 1.5 5.2 2.6 1.6 14 3.5 13 5.3 95 19 13 56 12 3 140 46 38 94 30 11 3.7 7.5 1.7 3.1 0.47 1.3 2 38 7 0.58 21 6.5 2.3 SM(d18:1/26:0) 5.5 2.7 1.2 5.2 4 1.5 5.1 7.9 8.5 3.5 1.9 37 15 6.3 20 9.1 5 55 19 14 42 12 5.7 9.5 4.8 3.6 7.7 1.6 2.3 1.2 0.85 0.29 16 1.8 9 3.8 2.2 SM(d18:0/16:0) 200 160 140 250 170 200 270 250 350 280 330 420 1300 930 1800 840 690 1000 2100 1600 3300 960 1500 230 270 270 210 240 430 110 93 110 120 83 160 450 250 550 350 330 430 SM(d18:0/18:0) 6.9 5 4.1 15 9.5 16 8.3 17 2.6 7.1 37 80 27 83 40 15 64 98 35 200 34 55 4.7 12 8.3 6.1 4.1 3.5 4.8 3.7 24 16 21 12 11 16 SM(d18:0/20:0) 9 1.2 2.7 3 15 13 14 3.7 19 3.4 18 39 32 38 28 37 25 72 74 100 32 42 5.5 2.7 24 6.1 0.99 1.1 7.1 1.6 20 14 9.4 11 11 13 SM(d18:0/22:0) 5.6 4.1 17 1.7 33 5.6 9.2 21 13 17 37 53 82 16 24 20 71 80 170 16 32 1.6 5.3 2 3.6 3.4 0.65 2.1 14 12 11 22 7.8 13 17 SM(d18:0/24:1) 58 15 20 42 28 25 62 37 88 59 100 110 590 240 810 230 180 190 730 380 1400 190 340 52 35 82 36 24 17 2.6 23 13 11 190 79 74 110 120 220 SM(d18:0/24:0) 8.1 1.2 4.1 5.3 5.2 9.7 8.6 6.6 1.6 4.5 82 19 67 30 14 28 76 22 220 23 53 5.8 2.7 26 2.9 6.1 3.7 17 7.1 18 11 11 31 SM(d18:0/26:1) 1.2 2 8.2 4.2 1.1 2.3 9.2 2.7 1.9 3.6 9.6 7.3 15 8.2 0.92 2.1 2 5.1 4.1 0.56 6.8 0.35 2.3 SM(d18:0/26:0) 0.77 0.92 2 3.4 1.4 0.74 4.1 20 6.4 5.3 8.7 2.2 5.1 3.9 19 7.7 7 1.5 1.8 3 3.2 15 3 0.56 0.77 2.3 Cholesterol 16000 12000 11000 8400 15000 25000 18000 22000 22000 18000 26000 39000 110000 94000 120000 83000 99000 100000 160000 180000 200000 160000 230000 190000 14000 13000 14000 12000 13000 14000 1700 950 1300 1100 1300 740 31000 38000 44000 39000 51000 49000 Desmosterol 85 85 78 100 240 190 110 170 140 210 430 230 660 760 890 1000 1900 800 860 1300 1200 1700 3900 1200 74 98 86 170 240 6.2 6.8 8.5 12 21 150 230 270 380 820 320 Lanosterol 4.2 4.2 3.8 220 220 280 15 11 11 420 390 470 43 16 36 650 620 860 42 32 32 700 680 780 250 220 1200 17 16 14 11 12 430 420 550 7-Dehydrocholesterol 44 25 96 33 160 200 260 69 68 80 220 320 230 88 130 140 53 35 45 90 22R-Hydroxycholesterol 5.9 8.5 10 49 59 73 44 100 24,25-Epoxycholesterol 8.3 8.4 7.6 3.7 13 12 14 21 19 17 32 19 29 31 41 26 48 31 31 55 49 29 71 39 3.6 5.4 6.9 5.5 8 0.47 0.23 0.59 0.4 0.63 9.4 16 19 16 25 15 25-Hydroxycholesterol 2.7 2.3 2.2 3.6 6.4 4.1 6.2 5.4 9.5 6.8 5.1 7.7 13 8.8 6.3 9.8 6.8 13 9.5 2.4 6.2 2.8 8.7 5.3 27-Hydroxycholesterol 4.3 5.9 11 3.6 7 12 14 22 8.4 6.8 11 13 9.5 31 8.7 11 10 17 37 8.2 12 5.6 15 16 26 7.5 7.7 4beta-Hydroxy-cholesterol 1.6 1.7 2.2 6.5 1.7 2.5 2 2.2 2 2.9 4.1 3.5 8.2 9.2 6.1 7.1 7.3 7.4 8.9 11 9.4 7.5 12 12 1.9 1.2 2.8 1.5 2 0.78 0.64 3.1 4.2 7.9 3.4 4.2 3.7 6alpha-Hydroxy-cholestanol 55 76 140 140 74 78 120 190 120 220 170 220 250 260 240 170 190 270 190 260 270 250 180 240 190 170 220 190 170 4.6 28 33 31 55 27 1.7 210 220 250 300 220 250 7-Oxo-cholesterol 42 32 20 27 41 53 51 66 36 58 94 83 110 110 98 130 150 140 160 160 130 140 180 150 27 20 43 26 26 2.5 2.3 1.9 16 2.1 66 37 46 43 56 66 66 7alpha-Hydroxy-cholesterol 5.6 11 4 9.9 7.7 10 5.9 8.7 2.8 11 15 8.5 7.3 14 9.6 13 9.4 13 15 16 50 13 8.3 12 2.6 10 4.7 5 6.2 5 7.8 7.9 6.8 7.2 7.8 Cholestenone 3.2 3.1 3.5 5.3 6.3 4.4 6.2 5 8.2 8.5 8.3 14 13 12 10 16 15 14 11 13 13 20 13 3.2 3.4 4.1 3.2 4.7 5.9 4.7 4.2 5.8 6.5 8.5 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name PA(30:1) PA(30:0) PA(32:4) PA(32:1) PA(32:0) PA(34:2) PA(34:1) PA(34:0) PA(36:3) PA(36:2) PA(36:1) PA(36:0) PA(38:5) PA(38:4) PA(38:3) PA(38:2) PA(38:1) PA(40:6) PA(40:4) PA(40:3) PA(40:1) PA(40:0) PC(28:0) PC(32:2) PC(32:1) PC(32:0) PC(34:3) PC(34:2) PC(34:1) PC(34:0) PC(36:4) PC(36:3) PC(36:2) PC(36:1) PC(36:0) PC(38:6) PC(38:5) PC(38:4) PC(38:2) PC(38:1) PC(38:0) PC(40:7) PC(40:6) PC(40:4) PC(40:2) PC(40:1) PC(42:2) PC(42:1) PE(32:0) PE(34:2) PE(34:1) PE(34:0) PE(36:4) PE(36:3) PE(36:2) PE(36:1) PE(36:0) PE(38:6) PE(38:5) PE(38:4) PE(38:3) PE(38:2) PE(38:1) PE(38:0) PE(40:6) PE(40:5) PE(40:4) PS(34:2) PS(34:1) PS(34:0) PS(36:4) PS(36:2) PS(36:1) PS(36:0) PS(38:5) PS(38:4) PS(38:3) PS(38:2) PS(38:1) PS(40:6) PS(40:5) PS(40:4) PS(40:3) PS(40:2) PS(40:1) PS(40:0) PS(42:4) PS(42:3) PS(42:2) PS(42:1) PS(42:0) PI(34:2) PI(34:1) PI(36:5) PI(36:4) PI(36:3) PI(36:2) PI(36:1) PI(36:0) PI(38:6) PI(38:5) PI(38:4) PI(38:3) PI(38:2) PI(38:1) PI(40:7) PI(40:6) PI(40:5) PI(40:4) PI(40:3) PG(32:1) PG(32:0) PG(34:3) PG(34:2) PG(34:1) PG(34:0) PG(36:4) PG(36:3) PG(36:2) PG(36:1) PG(38:5) PG(38:4) PG(38:3) PG(38:2) PG(38:1) PG(40:8) PG(40:7) PG(40:6) PG(40:5) PG(40:4) PG(40:3) PG(40:2) CL(66:5) CL(66:4) CL(66:3) CL(66:2) CL(68:5) CL(68:4) CL(68:3) CL(68:2) CL(70:6) CL(70:5) CL(70:4) CL(70:3) CL(72:7) CL(72:6) CL(72:5) CL(72:4) PC(P-36:4)/PC(O-36:5) PC(P-36:3)/PC(O-36:4) PC(P-36:2)/PC(O-36:3) PC(P-36:1)/PC(O-36:2) PC(P-38:4)/PC(O-38:5) PE(O-32:0) PE(P-36:4)/PE(O-36:5) PE(P-36:3)/PE(O-36:4) PE(P-36:2)/PE(O-36:3) PE(P-36:1)/PE(O-36:2) PE(P-36:0)/PE(O-36:1) PE(P-38:4)/PE(O-38:5) PE(P-38:2)/PE(O-38:3) PE(P-38:1)/PE(O-38:2) PE(P-38:0)/PE(O-38:1) Coenzyme Q9 Coenzyme Q10 Dolichol-18 Dolichol-19 Dolichol-20 Cer(d18:1/16:0) Cer(d18:1/18:0) Cer(d18:1/20:0) Cer(d18:1/22:0) Cer(d18:1/24:1) Cer(d18:1/24:0) Cer(d18:1/26:1) Cer(d18:1/26:0) Cer(d18:0/16:0) Cer(d18:0/18:0) Cer(d18:0/20:0) Cer(d18:0/22:0) Cer(d18:0/24:1) Cer(d18:0/24:0) Cer(d18:0/26:1) Cer(d18:0/26:0) HexCer(d18:1/16:0) HexCer(d18:1/18:0) HexCer(d18:1/20:0) HexCer(d18:1/22:0) HexCer(d18:1/24:1) HexCer(d18:1/24:0) HexCer(d18:1/26:1) HexCer(d18:1/26:0) HexCer(d18:0/16:0) HexCer(d18:0/18:0) HexCer(d18:0/20:0) HexCer(d18:0/22:0) HexCer(d18:0/24:1) HexCer(d18:0/24:0) HexCer(d18:0/26:1) HexCer(d18:0/26:0) SM(d18:1/16:0) SM(d18:1/18:0) SM(d18:1/20:0) SM(d18:1/22:0) SM(d18:1/24:1) SM(d18:1/24:0) SM(d18:1/26:1) SM(d18:1/26:0) SM(d18:0/16:0) SM(d18:0/18:0) SM(d18:0/20:0) SM(d18:0/22:0) SM(d18:0/24:1) SM(d18:0/24:0) SM(d18:0/26:1) SM(d18:0/26:0) Cholesterol Desmosterol Lanosterol 7-Dehydrocholesterol 22R-Hydroxycholesterol 24,25-Epoxycholesterol 25-Hydroxycholesterol 27-Hydroxycholesterol 4beta-Hydroxy-cholesterol 6alpha-Hydroxy-cholestanol 7-Oxo-cholesterol 7alpha-Hydroxy-cholesterol Cholestenone METABOLITES_END #END