#METABOLOMICS WORKBENCH jeffreymolendijk_20200326_144715 DATATRACK_ID:1956 STUDY_ID:ST001336 ANALYSIS_ID:AN002226 PROJECT_ID:000000 VERSION 1 CREATED_ON March 31, 2020, 3:18 pm #PROJECT PR:PROJECT_TITLE Effect of high-fat diet and bile acid treatment on serum and tissue lipidomes in PR:PROJECT_TITLE mice PR:PROJECT_SUMMARY We analyzed mouse serum and esophageal tissue samples from a mouse dietary PR:PROJECT_SUMMARY intervention experiment. Briefly, C57BL/6 mice (n=44) were divided into 4 groups PR:PROJECT_SUMMARY (n=11 per group) and fed High-fat diet (HFD), 0.2% deoxycholic acid (DCA) in PR:PROJECT_SUMMARY drinking water, both, or left as control for 9 months. The two treatments were PR:PROJECT_SUMMARY selected to demonstrate the ability of lipidomics to detect gross changes PR:PROJECT_SUMMARY induced by HFD in the serum and tissue lipidomes, as well as specific/minor PR:PROJECT_SUMMARY changes induced by the secondary bile acid (DCA) through regulation of liver PR:PROJECT_SUMMARY lipid metabolism. The serum and tissue samples were analyzed using targeted and PR:PROJECT_SUMMARY untargeted lipidomics methods. The targeted serum lipidomics data has previously PR:PROJECT_SUMMARY been uploaded as part of study ST001323. PR:INSTITUTE QIMR Berghofer Medical Research Institute PR:DEPARTMENT Precision & Systems Biomedicine PR:LAST_NAME Molendijk PR:FIRST_NAME Jeffrey PR:ADDRESS 300 Herston Road, Herston, QLD, 4006, Australia PR:EMAIL jeffrey.molendijk@qimrberghofer.edu.au PR:PHONE +61738453992 #STUDY ST:STUDY_TITLE Effect of high-fat diet and bile acid treatment on serum and tissue lipidomes in ST:STUDY_TITLE mice ST:STUDY_SUMMARY We analyzed mouse serum and esophageal tissue samples from a mouse dietary ST:STUDY_SUMMARY intervention experiment. Briefly, C57BL/6 mice (n=44) were divided into 4 groups ST:STUDY_SUMMARY (n=11 per group) and fed High-fat diet (HFD), 0.2% deoxycholic acid (DCA) in ST:STUDY_SUMMARY drinking water, both, or left as control for 9 months. For quality control,TQC ST:STUDY_SUMMARY samples and blanks were also included in the analysis. The two treatments were ST:STUDY_SUMMARY selected to demonstrate the ability of lipidomics to detect gross changes ST:STUDY_SUMMARY induced by HFD in the serum and tissue lipidomes, as well as specific/minor ST:STUDY_SUMMARY changes induced by the secondary bile acid (DCA) through regulation of liver ST:STUDY_SUMMARY lipid metabolism. The serum and tissue samples were analyzed using targeted and ST:STUDY_SUMMARY untargeted lipidomics methods. The targeted serum lipidomics data has previously ST:STUDY_SUMMARY been uploaded as part of study ST001323. ST:INSTITUTE QIMR Berghofer Medical Research Institute ST:LAST_NAME Molendijk ST:FIRST_NAME Jeffrey ST:ADDRESS 300 Herston Road, Herston, QLD, 4006, Australia ST:EMAIL jeffrey.molendijk@qimrberghofer.edu.au ST:PHONE +61738453992 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - F1_1A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_1A1.d SUBJECT_SAMPLE_FACTORS - F1_2A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_2A1.d SUBJECT_SAMPLE_FACTORS - F1_3A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_3A1.d SUBJECT_SAMPLE_FACTORS - F1_4A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_4A1.d SUBJECT_SAMPLE_FACTORS - F1_5A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_5A1.d SUBJECT_SAMPLE_FACTORS - F1_6A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_6A1.d SUBJECT_SAMPLE_FACTORS - F1_7A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_7A1.d SUBJECT_SAMPLE_FACTORS - F1_8A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_8A1.d SUBJECT_SAMPLE_FACTORS - F1_9A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_9A1.d SUBJECT_SAMPLE_FACTORS - F1_10A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_10A1.d SUBJECT_SAMPLE_FACTORS - F1_11A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_11A1.d SUBJECT_SAMPLE_FACTORS - F1_1B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_1B1.d SUBJECT_SAMPLE_FACTORS - F1_2B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_2B1.d SUBJECT_SAMPLE_FACTORS - F1_3B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_3B1.d SUBJECT_SAMPLE_FACTORS - F1_4B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_4B1.d SUBJECT_SAMPLE_FACTORS - F1_5B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_5B1.d SUBJECT_SAMPLE_FACTORS - F1_6B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_6B1.d SUBJECT_SAMPLE_FACTORS - F1_7B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_7B1.d SUBJECT_SAMPLE_FACTORS - F1_8B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_8B1.d SUBJECT_SAMPLE_FACTORS - F1_9B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_9B1.d SUBJECT_SAMPLE_FACTORS - F1_10B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_10B1.d SUBJECT_SAMPLE_FACTORS - F1_11B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_11B1.d SUBJECT_SAMPLE_FACTORS - F1_1C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_1C1.d SUBJECT_SAMPLE_FACTORS - F1_2C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_2C1.d SUBJECT_SAMPLE_FACTORS - F1_3C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_3C1.d SUBJECT_SAMPLE_FACTORS - F1_4C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_4C1.d SUBJECT_SAMPLE_FACTORS - F1_5C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_5C1.d SUBJECT_SAMPLE_FACTORS - F1_6C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_6C1.d SUBJECT_SAMPLE_FACTORS - F1_7C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_7C1.d SUBJECT_SAMPLE_FACTORS - F1_8C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_8C1.d SUBJECT_SAMPLE_FACTORS - F1_9C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_9C1.d SUBJECT_SAMPLE_FACTORS - F1_10C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_10C1.d SUBJECT_SAMPLE_FACTORS - F1_11C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_11C1.d SUBJECT_SAMPLE_FACTORS - F1_1D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_1D1.d SUBJECT_SAMPLE_FACTORS - F1_2D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_2D1.d SUBJECT_SAMPLE_FACTORS - F1_3D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_3D1.d SUBJECT_SAMPLE_FACTORS - F1_4D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_4D1.d SUBJECT_SAMPLE_FACTORS - F1_5D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_5D1.d SUBJECT_SAMPLE_FACTORS - F1_6D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_6D1.d SUBJECT_SAMPLE_FACTORS - F1_7D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_7D1.d SUBJECT_SAMPLE_FACTORS - F1_8D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_8D1.d SUBJECT_SAMPLE_FACTORS - F1_9D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_9D1.d SUBJECT_SAMPLE_FACTORS - F1_10D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_10D1.d SUBJECT_SAMPLE_FACTORS - F1_11D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F1_11D1.d SUBJECT_SAMPLE_FACTORS - F2_1A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_1A1.d SUBJECT_SAMPLE_FACTORS - F2_2A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_2A1.d SUBJECT_SAMPLE_FACTORS - F2_3A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_3A1.d SUBJECT_SAMPLE_FACTORS - F2_4A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_4A1.d SUBJECT_SAMPLE_FACTORS - F2_5A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_5A1.d SUBJECT_SAMPLE_FACTORS - F2_6A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_6A1.d SUBJECT_SAMPLE_FACTORS - F2_7A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_7A1.d SUBJECT_SAMPLE_FACTORS - F2_8A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_8A1.d SUBJECT_SAMPLE_FACTORS - F2_9A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_9A1.d SUBJECT_SAMPLE_FACTORS - F2_10A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_10A1.d SUBJECT_SAMPLE_FACTORS - F2_11A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_11A1.d SUBJECT_SAMPLE_FACTORS - F2_1B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_1B1.d SUBJECT_SAMPLE_FACTORS - F2_2B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_2B1.d SUBJECT_SAMPLE_FACTORS - F2_3B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_3B1.d SUBJECT_SAMPLE_FACTORS - F2_4B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_4B1.d SUBJECT_SAMPLE_FACTORS - F2_5B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_5B1.d SUBJECT_SAMPLE_FACTORS - F2_6B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_6B1.d SUBJECT_SAMPLE_FACTORS - F2_7B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_7B1.d SUBJECT_SAMPLE_FACTORS - F2_8B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_8B1.d SUBJECT_SAMPLE_FACTORS - F2_9B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_9B1.d SUBJECT_SAMPLE_FACTORS - F2_10B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_10B1.d SUBJECT_SAMPLE_FACTORS - F2_11B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_11B1.d SUBJECT_SAMPLE_FACTORS - F2_1C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_1C1.d SUBJECT_SAMPLE_FACTORS - F2_2C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_2C1.d SUBJECT_SAMPLE_FACTORS - F2_3C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_3C1.d SUBJECT_SAMPLE_FACTORS - F2_4C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_4C1.d SUBJECT_SAMPLE_FACTORS - F2_5C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_5C1.d SUBJECT_SAMPLE_FACTORS - F2_6C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_6C1.d SUBJECT_SAMPLE_FACTORS - F2_7C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_7C1.d SUBJECT_SAMPLE_FACTORS - F2_8C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_8C1.d SUBJECT_SAMPLE_FACTORS - F2_9C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_9C1.d SUBJECT_SAMPLE_FACTORS - F2_10C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_10C1.d SUBJECT_SAMPLE_FACTORS - F2_11C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_11C1.d SUBJECT_SAMPLE_FACTORS - F2_1D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_1D1.d SUBJECT_SAMPLE_FACTORS - F2_2D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_2D1.d SUBJECT_SAMPLE_FACTORS - F2_3D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_3D1.d SUBJECT_SAMPLE_FACTORS - F2_4D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_4D1.d SUBJECT_SAMPLE_FACTORS - F2_5D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_5D1.d SUBJECT_SAMPLE_FACTORS - F2_6D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_6D1.d SUBJECT_SAMPLE_FACTORS - F2_7D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_7D1.d SUBJECT_SAMPLE_FACTORS - F2_8D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_8D1.d SUBJECT_SAMPLE_FACTORS - F2_9D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_9D1.d SUBJECT_SAMPLE_FACTORS - F2_10D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_10D1.d SUBJECT_SAMPLE_FACTORS - F2_11D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=F2_11D1.d SUBJECT_SAMPLE_FACTORS - A1_1A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_1A1.d SUBJECT_SAMPLE_FACTORS - A1_2A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_2A1.d SUBJECT_SAMPLE_FACTORS - A1_3A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_3A1.d SUBJECT_SAMPLE_FACTORS - A1_4A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_4A1.d SUBJECT_SAMPLE_FACTORS - A1_5A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_5A1.d SUBJECT_SAMPLE_FACTORS - A1_6A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_6A1.d SUBJECT_SAMPLE_FACTORS - A1_7A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_7A1.d SUBJECT_SAMPLE_FACTORS - A1_8A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_8A1.d SUBJECT_SAMPLE_FACTORS - A1_9A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_9A1.d SUBJECT_SAMPLE_FACTORS - A1_10A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_10A1.d SUBJECT_SAMPLE_FACTORS - A1_11A1 Diet:Normal | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_11A1.d SUBJECT_SAMPLE_FACTORS - A1_1B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_1B1.d SUBJECT_SAMPLE_FACTORS - A1_2B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_2B1.d SUBJECT_SAMPLE_FACTORS - A1_3B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_3B1.d SUBJECT_SAMPLE_FACTORS - A1_4B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_4B1.d SUBJECT_SAMPLE_FACTORS - A1_5B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_5B1.d SUBJECT_SAMPLE_FACTORS - A1_6B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_6B1.d SUBJECT_SAMPLE_FACTORS - A1_7B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_7B1.d SUBJECT_SAMPLE_FACTORS - A1_8B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_8B1.d SUBJECT_SAMPLE_FACTORS - A1_9B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_9B1.d SUBJECT_SAMPLE_FACTORS - A1_10B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_10B1.d SUBJECT_SAMPLE_FACTORS - A1_11B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_11B1.d SUBJECT_SAMPLE_FACTORS - A1_1C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_1C1.d SUBJECT_SAMPLE_FACTORS - A1_2C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_2C1.d SUBJECT_SAMPLE_FACTORS - A1_3C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_3C1.d SUBJECT_SAMPLE_FACTORS - A1_4C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_4C1.d SUBJECT_SAMPLE_FACTORS - A1_5C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_5C1.d SUBJECT_SAMPLE_FACTORS - A1_6C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_6C1.d SUBJECT_SAMPLE_FACTORS - A1_7C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_7C1.d SUBJECT_SAMPLE_FACTORS - A1_8C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_8C1.d SUBJECT_SAMPLE_FACTORS - A1_9C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_9C1.d SUBJECT_SAMPLE_FACTORS - A1_10C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_10C1.d SUBJECT_SAMPLE_FACTORS - A1_11C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_11C1.d SUBJECT_SAMPLE_FACTORS - A1_1D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_1D1.d SUBJECT_SAMPLE_FACTORS - A1_2D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_2D1.d SUBJECT_SAMPLE_FACTORS - A1_3D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_3D1.d SUBJECT_SAMPLE_FACTORS - A1_4D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_4D1.d SUBJECT_SAMPLE_FACTORS - A1_5D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_5D1.d SUBJECT_SAMPLE_FACTORS - A1_6D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_6D1.d SUBJECT_SAMPLE_FACTORS - A1_7D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_7D1.d SUBJECT_SAMPLE_FACTORS - A1_8D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_8D1.d SUBJECT_SAMPLE_FACTORS - A1_9D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_9D1.d SUBJECT_SAMPLE_FACTORS - A1_10D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_10D1.d SUBJECT_SAMPLE_FACTORS - A1_11D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Targeted; RAW_FILE_NAME=A1_11D1.d SUBJECT_SAMPLE_FACTORS - TU_1A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_1A1.d SUBJECT_SAMPLE_FACTORS - TU_2A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_2A1.d SUBJECT_SAMPLE_FACTORS - TU_3A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_3A1.d SUBJECT_SAMPLE_FACTORS - TU_4A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_4A1.d SUBJECT_SAMPLE_FACTORS - TU_5A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_5A1.d SUBJECT_SAMPLE_FACTORS - TU_6A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_6A1.d SUBJECT_SAMPLE_FACTORS - TU_7A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_7A1.d SUBJECT_SAMPLE_FACTORS - TU_8A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_8A1.d SUBJECT_SAMPLE_FACTORS - TU_9A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_9A1.d SUBJECT_SAMPLE_FACTORS - TU_10A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_10A1.d SUBJECT_SAMPLE_FACTORS - TU_11A1 Diet:Normal | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_11A1.d SUBJECT_SAMPLE_FACTORS - TU_1B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_1B1.d SUBJECT_SAMPLE_FACTORS - TU_2B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_2B1.d SUBJECT_SAMPLE_FACTORS - TU_3B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_3B1.d SUBJECT_SAMPLE_FACTORS - TU_4B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_4B1.d SUBJECT_SAMPLE_FACTORS - TU_5B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_5B1.d SUBJECT_SAMPLE_FACTORS - TU_6B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_6B1.d SUBJECT_SAMPLE_FACTORS - TU_7B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_7B1.d SUBJECT_SAMPLE_FACTORS - TU_8B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_8B1.d SUBJECT_SAMPLE_FACTORS - TU_9B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_9B1.d SUBJECT_SAMPLE_FACTORS - TU_10B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_10B1.d SUBJECT_SAMPLE_FACTORS - TU_11B1 Diet:Normal | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_11B1.d SUBJECT_SAMPLE_FACTORS - TU_1C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_1C1.d SUBJECT_SAMPLE_FACTORS - TU_2C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_2C1.d SUBJECT_SAMPLE_FACTORS - TU_3C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_3C1.d SUBJECT_SAMPLE_FACTORS - TU_4C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_4C1.d SUBJECT_SAMPLE_FACTORS - TU_5C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_5C1.d SUBJECT_SAMPLE_FACTORS - TU_6C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_6C1.d SUBJECT_SAMPLE_FACTORS - TU_7C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_7C1.d SUBJECT_SAMPLE_FACTORS - TU_8C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_8C1.d SUBJECT_SAMPLE_FACTORS - TU_9C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_9C1.d SUBJECT_SAMPLE_FACTORS - TU_10C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_10C1.d SUBJECT_SAMPLE_FACTORS - TU_11C1 Diet:HighFat | BileAcid:water Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_11C1.d SUBJECT_SAMPLE_FACTORS - TU_1D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_1D1.d SUBJECT_SAMPLE_FACTORS - TU_2D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_2D1.d SUBJECT_SAMPLE_FACTORS - TU_3D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_3D1.d SUBJECT_SAMPLE_FACTORS - TU_4D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_4D1.d SUBJECT_SAMPLE_FACTORS - TU_5D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_5D1.d SUBJECT_SAMPLE_FACTORS - TU_6D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_6D1.d SUBJECT_SAMPLE_FACTORS - TU_7D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_7D1.d SUBJECT_SAMPLE_FACTORS - TU_8D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_8D1.d SUBJECT_SAMPLE_FACTORS - TU_9D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_9D1.d SUBJECT_SAMPLE_FACTORS - TU_10D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_10D1.d SUBJECT_SAMPLE_FACTORS - TU_11D1 Diet:HighFat | BileAcid:DCA Analysis=Tissue_Untargeted; RAW_FILE_NAME=QTOFPOS_MS_11D1.d SUBJECT_SAMPLE_FACTORS - SU_1A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S1A.d SUBJECT_SAMPLE_FACTORS - SU_2A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S2A.d SUBJECT_SAMPLE_FACTORS - SU_3A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S3A.d SUBJECT_SAMPLE_FACTORS - SU_4A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S4A.d SUBJECT_SAMPLE_FACTORS - SU_5A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S5A.d SUBJECT_SAMPLE_FACTORS - SU_6A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S6A.d SUBJECT_SAMPLE_FACTORS - SU_7A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S7A.d SUBJECT_SAMPLE_FACTORS - SU_8A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S8A.d SUBJECT_SAMPLE_FACTORS - SU_9A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S9A.d SUBJECT_SAMPLE_FACTORS - SU_10A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S10A.d SUBJECT_SAMPLE_FACTORS - SU_11A Diet:Normal | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S11A.d SUBJECT_SAMPLE_FACTORS - SU_1B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S1B.d SUBJECT_SAMPLE_FACTORS - SU_2B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S2B.d SUBJECT_SAMPLE_FACTORS - SU_3B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S3B.d SUBJECT_SAMPLE_FACTORS - SU_4B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S4B.d SUBJECT_SAMPLE_FACTORS - SU_5B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S5B.d SUBJECT_SAMPLE_FACTORS - SU_6B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S6B.d SUBJECT_SAMPLE_FACTORS - SU_7B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S7B.d SUBJECT_SAMPLE_FACTORS - SU_8B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S8B.d SUBJECT_SAMPLE_FACTORS - SU_9B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S9B.d SUBJECT_SAMPLE_FACTORS - SU_10B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S10B.d SUBJECT_SAMPLE_FACTORS - SU_11B Diet:Normal | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S11B.d SUBJECT_SAMPLE_FACTORS - SU_1C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S1C.d SUBJECT_SAMPLE_FACTORS - SU_2C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S2C.d SUBJECT_SAMPLE_FACTORS - SU_3C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S3C.d SUBJECT_SAMPLE_FACTORS - SU_4C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S4C.d SUBJECT_SAMPLE_FACTORS - SU_5C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S5C.d SUBJECT_SAMPLE_FACTORS - SU_6C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S6C.d SUBJECT_SAMPLE_FACTORS - SU_7C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S7C.d SUBJECT_SAMPLE_FACTORS - SU_8C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S8C.d SUBJECT_SAMPLE_FACTORS - SU_9C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S9C.d SUBJECT_SAMPLE_FACTORS - SU_10C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S10C.d SUBJECT_SAMPLE_FACTORS - SU_11C Diet:HighFat | BileAcid:water Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S11C.d SUBJECT_SAMPLE_FACTORS - SU_1D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S1D.d SUBJECT_SAMPLE_FACTORS - SU_2D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S2D.d SUBJECT_SAMPLE_FACTORS - SU_3D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S3D.d SUBJECT_SAMPLE_FACTORS - SU_4D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S4D.d SUBJECT_SAMPLE_FACTORS - SU_5D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S5D.d SUBJECT_SAMPLE_FACTORS - SU_6D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S6D.d SUBJECT_SAMPLE_FACTORS - SU_7D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S7D.d SUBJECT_SAMPLE_FACTORS - SU_8D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S8D.d SUBJECT_SAMPLE_FACTORS - SU_9D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S9D.d SUBJECT_SAMPLE_FACTORS - SU_10D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S10D.d SUBJECT_SAMPLE_FACTORS - SU_11D Diet:HighFat | BileAcid:DCA Analysis=Serum_Untargeted; RAW_FILE_NAME=POS_S11D.d #COLLECTION CO:COLLECTION_SUMMARY Mouse serum (30 µL) was added to 215 µL of ice-cold methanol containing 50 CO:COLLECTION_SUMMARY µg/mL butylated hydroxytoluene (BHT). Samples were homogenized by three rounds CO:COLLECTION_SUMMARY of vortex mixing for 30 seconds, freezing in liquid nitrogen for one minute, CO:COLLECTION_SUMMARY thawing for two minutes and sonicating for 10 minutes at 15°C, power 100% in a CO:COLLECTION_SUMMARY Grant XUB18 bath sonicator. Tissue wet weight was determined using a CO:COLLECTION_SUMMARY Mettler-Toledo XS105 balance (Mettler-Toledo, Melbourne, Australia). Biopsies CO:COLLECTION_SUMMARY were transferred to Eppendorf tubes containing 500 µL ice-cold methanol, 50 CO:COLLECTION_SUMMARY µg/ml BHT and one steel bead and homogenized in a TissueLyzer LT (Qiagen, CO:COLLECTION_SUMMARY Melbourne, Australia) for six minutes at 50 Hz. Homogenate was transferred to CO:COLLECTION_SUMMARY new tubes and the original tube was washed with 400 µL methanol and CO:COLLECTION_SUMMARY transferred. Samples were dried down under nitrogen flow and resuspended in 20 CO:COLLECTION_SUMMARY µL water and 200 µL methanol (50 µg/ml BHT). Samples were homogenized by CO:COLLECTION_SUMMARY three rounds of vortex mixing for 30 seconds, freezing in liquid nitrogen for CO:COLLECTION_SUMMARY one minute, thawing for two minutes and sonicating for 10 minutes at 15°C, CO:COLLECTION_SUMMARY power 100% in a Grant XUB18 bath sonicator. SPLASH LipidoMix Mass Spec Standard CO:COLLECTION_SUMMARY (10 µL) and Cer/Sph mixture II (10 µL) internal standards mixes from Avanti CO:COLLECTION_SUMMARY Polar Lipids were then added to each sample. After overnight incubation at CO:COLLECTION_SUMMARY -30°C, 750 µL MTBE was added and each tube was vortex mixed for 10 seconds and CO:COLLECTION_SUMMARY shaken for 10 minutes on a tube rotator (4°C). MilliQ water (188 µL) was then CO:COLLECTION_SUMMARY added, and the tube was vortex mixed for 30 seconds to form a biphasic CO:COLLECTION_SUMMARY separation. After centrifuging for 15 minutes at 15,000 × g, 700 µL of the CO:COLLECTION_SUMMARY clear upper phase containing lipids in MTBE was transferred to another tube and CO:COLLECTION_SUMMARY dried down using a gentle stream of nitrogen. After drying down of lipid CO:COLLECTION_SUMMARY extracts were resuspended in 50 µL methanol (containing 50 µg/mL BHT)/toluene CO:COLLECTION_SUMMARY (90/10%, v/v). CO:SAMPLE_TYPE Blood (serum) #TREATMENT TR:TREATMENT_SUMMARY C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat TR:TREATMENT_SUMMARY diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left as TR:TREATMENT_SUMMARY control for 9 months. The two treatments were selected to demonstrate the TR:TREATMENT_SUMMARY ability of lipidomics to detect gross changes induced by HFD in the serum TR:TREATMENT_SUMMARY lipidome, as well as specific/minor changes induced by the secondary bile acid TR:TREATMENT_SUMMARY (DCA) through regulation of liver lipid metabolism. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Mouse serum (30 µL) was added to 215 µL of ice-cold methanol containing 50 SP:SAMPLEPREP_SUMMARY µg/mL butylated hydroxytoluene (BHT). Samples were homogenized by three rounds SP:SAMPLEPREP_SUMMARY of vortex mixing for 30 seconds, freezing in liquid nitrogen for one minute, SP:SAMPLEPREP_SUMMARY thawing for two minutes and sonicating for 10 minutes at 15°C, power 100% in a SP:SAMPLEPREP_SUMMARY Grant XUB18 bath sonicator. Tissue wet weight was determined using a SP:SAMPLEPREP_SUMMARY Mettler-Toledo XS105 balance (Mettler-Toledo, Melbourne, Australia). Biopsies SP:SAMPLEPREP_SUMMARY were transferred to Eppendorf tubes containing 500 µL ice-cold methanol, 50 SP:SAMPLEPREP_SUMMARY µg/ml BHT and one steel bead and homogenized in a TissueLyzer LT (Qiagen, SP:SAMPLEPREP_SUMMARY Melbourne, Australia) for six minutes at 50 Hz. Homogenate was transferred to SP:SAMPLEPREP_SUMMARY new tubes and the original tube was washed with 400 µL methanol and SP:SAMPLEPREP_SUMMARY transferred. Samples were dried down under nitrogen flow and resuspended in 20 SP:SAMPLEPREP_SUMMARY µL water and 200 µL methanol (50 µg/ml BHT). Samples were homogenized by SP:SAMPLEPREP_SUMMARY three rounds of vortex mixing for 30 seconds, freezing in liquid nitrogen for SP:SAMPLEPREP_SUMMARY one minute, thawing for two minutes and sonicating for 10 minutes at 15°C, SP:SAMPLEPREP_SUMMARY power 100% in a Grant XUB18 bath sonicator. SPLASH LipidoMix Mass Spec Standard SP:SAMPLEPREP_SUMMARY (10 µL) and Cer/Sph mixture II (10 µL) internal standards mixes from Avanti SP:SAMPLEPREP_SUMMARY Polar Lipids were then added to each sample. After overnight incubation at SP:SAMPLEPREP_SUMMARY -30°C, 750 µL MTBE was added and each tube was vortex mixed for 10 seconds and SP:SAMPLEPREP_SUMMARY shaken for 10 minutes on a tube rotator (4°C). MilliQ water (188 µL) was then SP:SAMPLEPREP_SUMMARY added, and the tube was vortex mixed for 30 seconds to form a biphasic SP:SAMPLEPREP_SUMMARY separation. After centrifuging for 15 minutes at 15,000 × g, 700 µL of the SP:SAMPLEPREP_SUMMARY clear upper phase containing lipids in MTBE was transferred to another tube and SP:SAMPLEPREP_SUMMARY dried down using a gentle stream of nitrogen. After drying down of lipid SP:SAMPLEPREP_SUMMARY extracts were resuspended in 50 µL methanol (containing 50 µg/mL BHT)/toluene SP:SAMPLEPREP_SUMMARY (90/10%, v/v). SP:PROCESSING_STORAGE_CONDITIONS On ice #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1290 Infinity II CH:COLUMN_NAME Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS - #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Count MS_METABOLITE_DATA_START Samples F1_1A1 F1_2A1 F1_3A1 F1_4A1 F1_5A1 F1_6A1 F1_7A1 F1_8A1 F1_9A1 F1_10A1 F1_11A1 F1_1B1 F1_2B1 F1_3B1 F1_4B1 F1_5B1 F1_6B1 F1_7B1 F1_8B1 F1_9B1 F1_10B1 F1_11B1 F1_1C1 F1_2C1 F1_3C1 F1_4C1 F1_5C1 F1_6C1 F1_7C1 F1_8C1 F1_9C1 F1_10C1 F1_11C1 F1_1D1 F1_2D1 F1_3D1 F1_4D1 F1_5D1 F1_6D1 F1_7D1 F1_8D1 F1_9D1 F1_10D1 F1_11D1 Factors Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:water Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:Normal | BileAcid:DCA Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:water Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA Diet:HighFat | BileAcid:DCA 18:1-d9 SM 1083063 842280 787822 1139301 1310981 1167893 797784 2005942 1035506 1440092 1258255 1620617 843703 736157 1025256 2961665 868232 2085686 1275112 1299407 857885 1024557 1473524 1057407 637855 2501070 2128561 1685375 1966819 1251642 715728 733484 996363 815969 753798 1218233 1020879 399925 1080449 493074 382012 339447 208401 595604 SM 18:1/16:0 46011172 47419908 32804440 39352660 35309176 55410596 49165920 45651736 39868940 44209300 43299916 51841096 54871776 37775492 44942704 54548072 52055136 49633276 43289060 51552272 36125824 55096164 47317732 47611384 29344170 55599928 55405624 41371700 57572780 51543616 41564808 43460580 41026148 41998828 48852976 48917228 54208488 31010034 56511700 41468732 36537728 41487336 13532167 48479444 SM 18:1/16:1 2237662 1934401 986414 1874057 1802363 1941064 1821602 2253460 1828790 2309153 1623520 1920052 2073942 1264958 2403176 1803878 1736178 2362686 2422108 2297548 1641944 1880815 1753456 2001722 1195155 1616839 2094868 1798722 1558444 2143943 1927900 1624774 1938439 1611846 2076363 2242520 2230122 2066819 2370314 2060374 1719363 2080566 532579 1918914 SM 18:1/16:2 2552092 2523128 1514868 3511734 2697887 2565238 2692407 3395875 3700730 2931463 1895037 4123972 2229878 1242675 3771325 3319485 3119371 3650640 3664153 3777409 2444127 4379076 3563076 3572372 1020753 3251159 4381889 3275689 2611842 2858058 2332500 2187569 3013011 2239102 3151153 1998402 4380251 1406297 3010053 2017068 1162481 2591661 460417 2326724 SM 18:1/18:0 19207232 9871836 8523347 18310744 19633176 9033085 9450197 20887284 20446470 17087466 10678055 18180916 13064014 8643824 18471660 12535070 12125102 18265410 10642818 16975576 15185150 13846158 22506018 14030047 7498802 16694124 25858208 17485304 18202504 16656257 13712707 14366892 15605369 11829562 18234690 14682691 19163842 8217863 18326084 13054085 12734676 14069764 4837099 18086606 SM 18:1/18:1 2724413 1388842 1065603 2742186 2683390 1308819 1586496 3158601 2899381 2556740 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144481 147224 122331 74832 212498 107823 125742 162930 143550 55510 114291 85820 322972 66755 144070 103471 142708 108589 93347 182095 114903 80415 238163 152547 207479 200533 142085 82116 77990 95294 88880 76711 134868 78529 41512 104654 48646 39783 29751 49802 64810 PC 36:0 473534 397613 470552 647576 585340 412633 421504 437878 648327 466525 458140 406918 260151 396647 389201 440561 417841 431709 420880 571634 614504 405772 700956 520471 412278 624442 616006 663980 509289 470360 516093 563977 510427 506298 502662 505287 398477 490583 443691 531101 489242 579635 417186 603286 PC 36:1 5128187 3986061 4780081 5494167 6032100 3206320 3701458 4596603 5535180 4297124 3967146 4261467 3108958 3521136 4353937 4314781 3826600 3868062 3796536 5583111 5591808 4195207 6297664 4989824 3961939 5155495 5231854 5449161 4418894 3835724 4328532 5124260 4576992 4794892 4590239 4878469 4116972 3977603 4412800 4578245 4374024 5182553 3464316 5647686 PC 36:2 8292417 8291130 8120818 9702042 9717800 6739269 7735350 9650921 11835458 9212188 6814926 8974853 6991103 5949843 10074565 8654653 8764533 9215417 10063364 7762238 9102346 7803534 9047661 6739527 6568122 9729006 8763677 7766581 7608601 7275228 7804988 9229106 7671760 9769581 9757428 7344815 6974210 7578776 6313969 8063632 8595811 9170134 4962457 10786642 PC 36:3 7538312 6823294 5973050 7305474 6533697 6390622 6236884 8236522 7977655 7347501 5514339 6681224 5760157 4803685 7634987 6308581 7025776 7758935 6588212 6078169 6780962 6769908 5243750 4730993 4384586 6932443 6107094 5610612 5430596 4630621 4331763 5357391 6188483 8416980 6703675 7211947 5747619 8463203 3620385 8834430 5626183 7227059 3307296 7151347 PC 36:4 9944078 8429794 11209480 10199506 12149489 7429421 7980580 11816486 15650141 10831948 7450482 9680780 6409175 6655381 10303775 6791198 9011647 11047558 7709160 8322368 10431594 8987566 11790337 8680013 8157946 18124926 14322908 10823715 10352111 9399678 10139642 12427665 11881769 15144739 11136559 12914013 9662632 16568464 6461117 15121964 13481118 13568145 6548886 12658602 PC 36:5 629092 491812 419194 610645 678323 539230 571529 799643 763105 593297 366367 615345 440491 262811 802823 453599 500585 962024 755218 564514 550385 570175 419180 370535 291653 596176 579472 447340 491605 345178 381796 430201 436224 711686 580791 823519 549028 846480 383579 810858 602240 729514 172402 582914 PC 38:1 83776 68836 95898 139472 128449 72947 82513 92366 149643 106818 71564 115852 79068 81906 91448 141743 96482 97529 89419 128335 119940 96148 106874 84826 51977 97087 127566 92057 64341 60823 83037 79298 85843 90673 87120 68171 77559 103635 78622 104639 83672 97898 50234 109808 PC 38:2 306422 242227 270860 470623 341183 245674 301839 303065 454277 322300 235750 366605 250140 259803 331495 376739 358743 342242 304245 411452 373510 355732 315684 234026 168655 314485 327892 243267 193462 249653 246654 269927 230202 325820 273202 199337 201367 241975 181556 270471 285553 322218 139383 330975 PC 38:3 1231285 802308 1034424 1548151 1696311 666018 859719 1382954 1725913 1183817 822322 1371912 824391 826371 1401436 1070084 1251657 1359278 1119335 1364116 1798278 1224096 1398552 1042776 862078 1274675 1164781 1143207 927754 894860 969447 1169968 1082609 1558609 1293075 1256604 832961 1235524 745855 1165457 1160707 1259156 651167 1339788 PC 38:4 4835856 4469685 4502418 6680759 6465375 3288506 3895577 5782579 6341473 4634605 4041579 5149255 3956538 2389201 5503754 4104838 4240365 5023029 4735303 5349756 5837129 4240418 7026118 5242938 4110653 6361248 6932360 6787348 5810726 5312130 5560018 6898384 5020455 7121753 6278147 6363392 5109231 6129954 5049227 6664126 5918067 7114837 3181986 7248245 PC 38:5 2147424 1393936 1551931 2275708 2959170 1440737 1530199 2618634 3121359 1994377 1185668 1989759 1522465 944686 2569147 1266276 1740523 2745642 1821122 1914885 1965745 1994086 1804361 1549099 1166484 1479482 1918055 2015624 1672129 1355000 1738961 2053450 1963965 2384897 2022945 2368519 1870173 2575645 1180860 2775282 2034750 2397131 936332 2186295 PC 38:6 2996110 1610718 1497525 3323680 3528292 1467844 1476286 3421564 3779797 2278974 1305226 3636253 2205354 1213917 3533715 1635158 2388856 3826780 2805904 2881933 2618193 2373595 2131746 1879040 1459575 1636841 2235285 2207207 1530258 1754089 2116257 2581268 2417570 3975546 2290536 2181937 1663539 2150785 1552622 2758708 2340693 2666045 1195871 2799301 PC 40:1 74853 37928 78952 121766 99234 21077 61451 79576 100214 97398 48965 75733 45683 36004 67355 100530 56290 75636 66451 96414 101752 84568 89942 60630 31565 93556 91449 72727 40786 43388 55448 32698 65842 35199 52321 32694 57535 65023 62665 108681 54670 64993 37336 56890 PC 40:2 67296 49641 79767 129684 84622 36395 66244 78162 97915 94189 55881 84746 42129 46720 83168 114991 70785 88482 67439 80429 74618 82307 72395 46494 32418 94543 85607 56332 35611 38858 42208 41196 60874 47748 53074 29157 41311 45918 47545 82929 53549 52020 30333 73271 PC 40:3 91803 38033 56298 123385 93125 39786 48242 85864 117178 99324 43031 117335 58876 51323 96572 59411 86747 73764 67437 110258 122311 109223 95891 62986 40189 55417 87973 72466 53927 76658 75451 68194 79383 59600 74251 32522 57896 58944 53837 74508 68490 68994 38626 65928 PC 40:4 536049 139765 186291 771064 590947 111970 167958 483208 662015 534443 160756 516203 318699 265274 504114 174412 430031 364533 273093 547339 747745 508083 644296 403670 262362 261471 499604 556707 373152 476932 462227 487245 490936 344791 569628 155836 350314 326227 383150 453865 397495 410808 224044 458342 PC 40:5 697990 259725 317041 780326 852445 212820 288602 952741 833969 533431 262996 622297 512762 238734 717319 231624 530898 540628 384182 666965 770595 592853 592104 362814 216935 358663 587989 487163 425953 450946 471136 500114 569835 387120 555579 270297 449853 403297 366469 541744 460529 490313 216343 588655 PC 40:6 1786794 484608 522827 1807363 1535502 322316 505747 1475000 1771597 1333561 410222 1505590 968994 514561 1618556 576158 929590 1452483 1160155 1517560 1610800 1263931 1445343 803264 429871 786981 1537790 1114097 656380 1111445 1244911 1202578 1434955 1073768 1368078 669912 1047355 869422 809046 1223792 1040943 1237764 345210 1337655 PC 40:7 588369 117303 162373 361963 461737 111199 152716 528897 562811 338401 121462 453479 327790 82724 626633 125718 227335 513352 326911 340059 466604 325003 286945 156959 86614 132994 286070 211037 137590 233055 298802 227768 319251 375581 396225 221818 273123 244791 188548 276819 259388 311553 66121 313528 PC 40:8 238736 71874 88627 176117 267768 69107 84387 281600 282384 150538 62939 239197 140372 43649 277782 85503 98368 206937 127707 126624 135940 124133 118475 91881 47279 84170 210987 142148 95590 102052 124153 118113 157593 124488 176657 122499 137476 114423 78446 148824 124121 157015 25951 145774 15:0-18:1(d7) PC 3713254 2555092 3377573 3630337 4127299 3902704 2421454 5732358 3674491 4012625 5271826 4431991 1835813 3040494 2636371 9812525 2632651 5227362 3329234 4448143 3274935 3037122 5140791 3141245 2477369 6538547 5014215 5752662 5534230 3902417 2572139 2802161 2888762 2779107 2342537 4512742 2401516 1400234 3333406 1606010 1373174 1150269 1219339 1928441 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name RefMet name Formula Mass MW structure 18:1-d9 SM SM 18:1/16:0 SM 18:1/16:1 SM 18:1/16:2 SM 18:1/18:0 SM 18:1/18:1 SM 18:1/18:2 SM 18:1/20:0 SM 18:1/20:1 SM 18:1/20:2 SM 18:1/22:0 SM 18:1/22:1 SM 18:1/22:2 SM 18:1/22:3 SM 18:1/22:5 SM 18:1/22:6 SM 18:1/24:0 SM 18:1/24:1 SM 18:1/24:2 SM 18:1/26:1 SM d18:0/16:0 SM(d18:0/16:0) C39H81N2O6P 704.5832 30729 SM d18:0/18:0 SM(d18:0/18:0) C41H85N2O6P 732.6145 30736 SM d18:0/20:0 SM(d18:0/20:0) C43H89N2O6P 760.6458 30737 SM d18:0/22:0 SM(d18:0/22:0) C45H93N2O6P 788.6771 30738 SM d18:0/24:0 SM(d18:0/24:0) C47H97N2O6P 816.7084 30740 Cer d18:0/C16:0 Cer d18:0/C24:1 Cer d18:0/C24:2 Cer d18:0/C26:2 Cer d18:1/C12:0 Cer d18:1/C16:0 Cer d18:1/C16:1 Cer d18:1/C18:0 Cer d18:1/C18:1 Cer d18:1/C20:0 Cer d18:1/C20:1 Cer d18:1/C20:2 Cer d18:1/C22:0 Cer d18:1/C22:1 Cer d18:1/C22:2 Cer d18:1/C22:3 Cer d18:1/C22:4 Cer d18:1/C22:5 Cer d18:1/C22:6 Cer d18:1/C24:0 Cer d18:1/C24:1 Cer d18:1/C24:2 Cer d18:1/C26:0 Cer d18:1/C26:1 Cer d18:2/C16:0 Cer d18:2/C16:1 Cer d18:2/C18:0 Cer d18:2/C18:1 Cer d18:2/C20:0 Cer d18:2/C22:0 Cer d18:2/C24:1 Cer d18:2/C24:2 PC 30:0 PC(30:0) C38H76NO8P 705.5309 PC 32:0 PC(32:0) C40H80NO8P 733.5622 PC 32:1 PC(32:1) C40H78NO8P 731.5465 PC 32:2 PC(32:2) C40H76NO8P 729.5309 PC 32:3 PC(32:3) C40H74NO8P 727.5152 PC 34:0 PC(34:0) C42H84NO8P 761.5935 PC 34:1 PC(34:1) C42H82NO8P 759.5778 PC 34:2 PC(34:2) C42H80NO8P 757.5622 PC 34:3 PC(34:3) C42H78NO8P 755.5465 PC 34:4 PC(34:4) C42H76NO8P 753.5309 PC 34:5 PC(34:5) C42H74NO8P 751.5152 PC 36:0 PC(36:0) C44H88NO8P 789.6248 PC 36:1 PC(36:1) C44H86NO8P 787.6091 PC 36:2 PC(36:2) C44H84NO8P 785.5935 PC 36:3 PC(36:3) C44H82NO8P 783.5778 PC 36:4 PC(36:4) C44H80NO8P 781.5622 PC 36:5 PC(36:5) C44H78NO8P 779.5465 PC 38:1 PC(38:1) C46H90NO8P 815.6404 PC 38:2 PC(38:2) C46H88NO8P 813.6248 PC 38:3 PC(38:3) C46H86NO8P 811.6091 PC 38:4 PC(38:4) C46H84NO8P 809.5935 PC 38:5 PC(38:5) C46H82NO8P 807.5778 PC 38:6 PC(38:6) C46H80NO8P 805.5622 PC 40:1 PC(40:1) C48H94NO8P 843.6717 PC 40:2 PC(40:2) C48H92NO8P 841.6561 PC 40:3 PC(40:3) C48H90NO8P 839.6404 PC 40:4 PC(40:4) C48H88NO8P 837.6248 PC 40:5 PC(40:5) C48H86NO8P 835.6091 PC 40:6 PC(40:6) C48H84NO8P 833.5935 PC 40:7 PC(40:7) C48H82NO8P 831.5778 PC 40:8 PC(40:8) C48H80NO8P 829.5622 15:0-18:1(d7) PC METABOLITES_END #END