#METABOLOMICS WORKBENCH htomatsu_20201202_234640 DATATRACK_ID:2341 STUDY_ID:ST001684 ANALYSIS_ID:AN002750 PROJECT_ID:000000 VERSION 1 CREATED_ON December 21, 2020, 7:38 pm #PROJECT PR:PROJECT_TITLE An overexpression of lipoprotein lipase leads to an alteration in the skeletal PR:PROJECT_TITLE muscle metabolome in transgenic rabbits PR:PROJECT_SUMMARY The purpose of the current study was to characterize the skeletal muscle PR:PROJECT_SUMMARY metabolome in the lipoprotein lipase (LPL) transgenic rabbits. The skeletal PR:PROJECT_SUMMARY muscle metabolite profile was analyzed using capillary electrophoresis time-of PR:PROJECT_SUMMARY flight mass spectrometry in the control rabbits and LPL transgenic rabbits (n = PR:PROJECT_SUMMARY 9, each group). PR:INSTITUTE Saga University PR:LAST_NAME Nishida PR:FIRST_NAME Yuichiro PR:ADDRESS Department of Preventive Medicine, Faculty of Medicine, Saga University, 5-1-1 PR:ADDRESS Nabeshima, Saga 849-8501 PR:EMAIL ynishida@cc.saga-u.ac.jp PR:PHONE +81 952 34 2287 #STUDY ST:STUDY_TITLE An overexpression of lipoprotein lipase leads to an alteration in the skeletal ST:STUDY_TITLE muscle metabolome in transgenic rabbits ST:STUDY_SUMMARY The purpose of the current study was to characterize the skeletal muscle ST:STUDY_SUMMARY metabolome in the lipoprotein lipase (LPL) transgenic rabbits. The skeletal ST:STUDY_SUMMARY muscle metabolite profile was analyzed using capillary electrophoresis time-of ST:STUDY_SUMMARY flight mass spectrometry in the control rabbits and LPL transgenic rabbits (n = ST:STUDY_SUMMARY 9, each group). ST:INSTITUTE Saga University ST:LAST_NAME Nishida ST:FIRST_NAME Yuichiro ST:ADDRESS Department of Preventive Medicine, Faculty of Medicine, Saga University, 5-1-1 ST:ADDRESS Nabeshima, Saga 849-8501 ST:EMAIL ynishida@cc.saga-u.ac.jp ST:PHONE +81 952 34 2287 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Oryctolagus cuniculus #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 2311 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2312 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2313 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2314 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2315 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2316 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2258 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2284 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2285 Genotype:Transgenic SUBJECT_SAMPLE_FACTORS - 2317 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2318 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2319 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2320 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2322 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2260 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2262 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2287 Genotype:Wild-type SUBJECT_SAMPLE_FACTORS - 2289 Genotype:Wild-type #COLLECTION CO:COLLECTION_SUMMARY Immediately after the scarification, gastrocnemius muscle was dissected. These CO:COLLECTION_SUMMARY muscle samples were immediately frozen in liquid nitrogen, and stored at -80 CO:COLLECTION_SUMMARY degree until a metabolome analysis. CO:SAMPLE_TYPE Muscle #TREATMENT TR:TREATMENT_SUMMARY At the end of high fat diet feeding for 16 weeks (40 weeks old), after overnight TR:TREATMENT_SUMMARY fasting, rabbits were sacrificed between 08:30 and 12:30 by overdose of TR:TREATMENT_SUMMARY thiamylal sodium. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) SP:SAMPLEPREP_SUMMARY containing internal standards (20 μM) and homogenized by a homogenizer (4500 SP:SAMPLEPREP_SUMMARY rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through SP:SAMPLEPREP_SUMMARY 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, SP:SAMPLEPREP_SUMMARY Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally SP:SAMPLEPREP_SUMMARY concentrated and resuspended in 50 µL of ultrapure water immediately before the SP:SAMPLEPREP_SUMMARY measurement. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Other CH:INSTRUMENT_NAME Agilent CE CH:COLUMN_NAME Agilent Fused silica capillary (800 mm x 50 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent CE-TOFMS MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS - #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Relative Area MS_METABOLITE_DATA_START Samples 2311 2312 2313 2314 2315 2316 2258 2284 2285 2317 2318 2319 2320 2322 2260 2262 2287 2289 Factors Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Transgenic Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Pyruvic acid 6.3E-03 8.3E-03 6.4E-03 8.2E-03 7.7E-03 6.4E-03 7.4E-03 7.9E-03 6.6E-03 6.6E-03 7.9E-03 6.0E-03 6.6E-03 7.1E-03 7.6E-03 6.7E-03 7.2E-03 5.8E-03 Lactic acid 8.3E-01 7.1E-01 7.3E-01 1.0E+00 1.0E+00 1.5E+00 1.0E+00 1.9E+00 7.7E-01 1.2E+00 1.4E+00 8.6E-01 9.0E-01 1.7E+00 1.0E+00 1.2E+00 1.1E+00 1.3E+00 3-Hydroxybutyric acid 1.3E-03 1.9E-03 1.7E-03 1.2E-03 1.3E-03 2.1E-03 9.9E-04 1.1E-03 1.4E-03 9.8E-04 1.0E-03 1.4E-03 1.8E-03 1.0E-03 1.7E-03 1.4E-03 1.7E-03 1.1E-03 2-Hydroxyisobutyric acid 6.1E-04 4.8E-04 4.7E-04 4.5E-04 4.2E-04 7.3E-04 6.4E-04 3.3E-04 3.8E-04 5.7E-04 4.1E-04 4.7E-04 4.8E-04 5.1E-04 4.8E-04 5.0E-04 8.8E-04 4.3E-04 Fumaric acid 5.6E-03 3.5E-03 4.5E-03 4.3E-03 3.2E-03 5.4E-03 5.7E-03 3.3E-03 3.3E-03 2.6E-03 3.9E-03 3.1E-03 3.7E-03 3.6E-03 4.5E-03 1.7E-03 2.8E-03 3.2E-03 Succinic acid 1.6E-02 1.1E-02 1.6E-02 1.2E-02 9.0E-03 2.5E-02 1.2E-02 1.3E-02 1.3E-02 9.0E-03 1.1E-02 1.2E-02 1.4E-02 1.4E-02 1.4E-02 8.4E-03 1.4E-02 2.7E-02 2-Hydroxyethanesulfonate 5.0E-04 2.5E-04 2.8E-04 1.9E-04 3.5E-04 2.5E-04 4.1E-04 3.2E-04 3.1E-04 3.2E-04 4.0E-04 5.6E-04 5.4E-04 1.8E-04 3.9E-04 2.0E-04 3.3E-04 3.8E-04 Pyroglutamic acid 7.1E-04 6.1E-04 6.5E-04 8.0E-04 4.5E-04 7.2E-04 7.1E-04 5.4E-04 5.5E-04 4.7E-04 7.1E-04 7.8E-04 6.3E-04 6.6E-04 6.4E-04 3.6E-04 8.8E-04 6.4E-04 129.055340909091_9.182088 7.7E-04 7.4E-04 N.D. 7.3E-04 7.2E-04 N.D. 7.5E-04 8.3E-04 8.1E-04 6.6E-04 N.D. N.D. 6.6E-04 7.5E-04 N.D. N.D. 7.5E-04 N.D. Glutaric acid N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 2.9E-03 N.D. N.D. N.D. Malic acid 4.5E-02 3.2E-02 3.8E-02 3.4E-02 2.8E-02 5.0E-02 5.0E-02 2.9E-02 3.2E-02 2.3E-02 3.3E-02 2.7E-02 3.0E-02 3.3E-02 4.0E-02 1.6E-02 2.4E-02 2.8E-02 Phosphoethanolamine 1.9E-03 2.3E-03 1.9E-03 2.0E-03 2.1E-03 2.2E-03 2.4E-03 1.9E-03 2.1E-03 1.7E-03 2.0E-03 2.5E-03 2.3E-03 1.7E-03 2.2E-03 2.7E-03 2.4E-03 2.0E-03 Caprylic acid 4.4E-04 2.8E-04 N.D. N.D. N.D. 2.6E-04 N.D. 2.5E-04 N.D. 2.6E-04 4.4E-04 N.D. 3.6E-04 2.4E-04 N.D. N.D. N.D. N.D. Oxoglutaric acid 2.2E-03 2.3E-03 1.9E-03 2.0E-03 1.9E-03 N.D. 3.4E-03 N.D. N.D. N.D. 1.5E-03 N.D. N.D. N.D. N.D. N.D. N.D. N.D. Adipic acid N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 1.6E-03 N.D. N.D. N.D. 2-Hydroxyglutaric acid 5.9E-04 5.9E-04 5.2E-04 6.2E-04 7.0E-04 2.9E-04 6.7E-04 4.0E-04 4.6E-04 3.6E-04 5.0E-04 4.6E-04 4.2E-04 3.9E-04 4.5E-04 N.D. 4.0E-04 2.9E-04 Pelargonic acid 1.1E-03 7.7E-04 1.1E-03 7.6E-04 9.3E-04 7.1E-04 7.8E-04 7.6E-04 1.1E-03 9.0E-04 1.3E-03 1.1E-03 1.1E-03 9.2E-04 1.1E-03 7.9E-04 9.9E-04 8.3E-04 Terephthalic acid 6.4E-04 5.6E-04 6.3E-04 4.7E-04 5.3E-04 4.1E-04 5.4E-04 6.8E-04 1.4E-03 5.2E-04 5.3E-04 5.8E-04 6.2E-04 4.4E-04 5.3E-04 6.3E-04 4.5E-04 4.5E-04 Phosphoenolpyruvic acid N.D. 2.1E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Uric acid 9.2E-05 2.0E-04 6.0E-05 N.D. N.D. 1.3E-04 1.4E-04 6.5E-04 3.6E-04 1.5E-04 1.9E-04 1.6E-04 N.D. 6.5E-05 1.0E-04 N.D. 2.2E-04 N.D. Dihydroxyacetone phosphate 7.8E-03 1.9E-02 5.9E-03 1.3E-02 1.4E-02 1.2E-02 2.1E-02 2.8E-02 4.7E-03 7.2E-03 3.8E-02 7.5E-03 3.7E-03 1.0E-02 1.4E-02 1.5E-02 4.4E-03 1.3E-02 Glyceraldehyde 3-phosphate 2.1E-04 3.3E-04 N.D. N.D. 2.1E-04 N.D. 2.8E-04 7.6E-04 2.1E-04 2.5E-04 5.1E-04 N.D. N.D. N.D. 3.8E-04 4.1E-04 N.D. 4.1E-04 sn-Glycero-3-phosphate 3.6E-02 3.5E-02 3.3E-02 2.7E-02 4.0E-02 5.8E-02 4.5E-02 4.6E-02 3.2E-02 3.6E-02 3.6E-02 3.7E-02 2.9E-02 3.7E-02 4.5E-02 4.3E-02 4.5E-02 4.8E-02 N-Acetylaspartic acid 4.1E-04 3.6E-04 7.8E-04 5.1E-04 2.5E-04 4.9E-04 3.4E-04 1.8E-04 7.8E-04 2.5E-04 7.0E-04 1.9E-03 5.5E-04 2.1E-04 7.6E-04 3.4E-04 1.6E-03 8.1E-04 Vitamin C N.D. N.D. N.D. N.D. 4.1E-04 8.0E-04 6.2E-04 3.1E-04 9.5E-04 4.1E-04 N.D. 3.3E-04 N.D. N.D. 5.7E-04 3.0E-04 N.D. N.D. Pyrophosphate 5.4E-03 N.D. 4.4E-03 5.5E-03 4.7E-03 4.6E-03 4.2E-03 4.2E-03 5.4E-03 3.6E-03 4.2E-03 4.8E-03 4.9E-03 5.6E-03 5.0E-03 N.D. 4.9E-03 4.6E-03 3-Phosphoglyceric acid 7.8E-04 1.5E-03 6.0E-04 1.1E-03 7.7E-04 6.6E-04 1.3E-03 1.7E-03 7.2E-04 6.9E-04 1.3E-03 6.0E-04 6.0E-04 5.7E-04 5.0E-04 7.0E-04 7.5E-04 6.0E-04 186.113085555556_7.54518425555555 4.1E-04 4.0E-04 5.0E-04 4.4E-04 5.1E-04 4.0E-04 4.7E-04 4.8E-04 4.7E-04 3.5E-04 4.2E-04 4.4E-04 4.1E-04 4.6E-04 3.6E-04 3.6E-04 5.0E-04 4.0E-04 Azelaic acid N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 1.2E-03 1.5E-04 2.7E-04 N.D. N.D. N.D. 4.7E-04 N.D. 1.1E-03 N.D. 191.018935_7.97564147777778 6.9E-04 4.1E-04 7.9E-04 5.4E-04 4.5E-04 3.1E-04 3.7E-04 3.4E-04 4.1E-04 3.9E-04 3.9E-04 4.8E-04 6.1E-04 4.3E-04 5.4E-04 7.3E-04 7.8E-04 6.7E-04 Citric acid 2.3E-02 3.6E-02 2.0E-02 3.0E-02 1.9E-02 1.9E-02 3.5E-02 2.6E-02 1.6E-02 1.5E-02 1.9E-02 2.1E-02 1.5E-02 1.6E-02 2.7E-02 1.9E-02 1.3E-02 1.1E-02 Quinic acid N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 4.1E-04 N.D. 1.5E-04 N.D. Phenylacetylglycine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 5.5E-04 N.D. 1.4E-04 N.D. N.D. N.D. N.D. Galacturonic acid N.D. N.D. N.D. N.D. N.D. N.D. N.D. 2.1E-04 3.8E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. 7.5E-04 N.D. Gluconic acid 2.0E-04 1.6E-04 1.8E-04 1.5E-04 1.5E-04 1.7E-04 3.9E-04 2.3E-04 6.3E-04 4.9E-04 N.D. 2.4E-04 N.D. 1.6E-04 4.0E-04 1.8E-04 7.4E-04 1.9E-04 Lauric acid 1.1E-03 6.3E-04 8.1E-04 N.D. 1.0E-03 5.7E-04 5.6E-04 N.D. 1.1E-03 1.0E-03 8.4E-04 1.0E-03 1.2E-03 7.2E-04 9.8E-04 N.D. 1.1E-03 7.5E-04 Phosphocreatine 2.8E-01 3.1E-01 3.1E-01 2.7E-01 2.3E-01 1.2E-01 2.6E-01 1.4E-01 2.4E-01 2.3E-01 1.5E-01 2.3E-01 1.8E-01 1.2E-01 1.9E-01 1.9E-01 2.2E-01 1.1E-01 Indoxyl sulfate N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 4.1E-04 N.D. N.D. N.D. N.D. N.D. N.D. Pantothenic acid 2.1E-03 2.1E-03 2.0E-03 1.9E-03 1.6E-03 1.5E-03 2.3E-03 1.5E-03 1.7E-03 1.1E-03 2.0E-03 1.4E-03 1.9E-03 1.7E-03 1.5E-03 1.8E-03 1.2E-03 1.5E-03 Ribulose 5-phosphate 8.9E-04 1.2E-03 8.8E-04 7.7E-04 7.5E-04 7.9E-04 1.5E-03 8.5E-04 7.5E-04 5.8E-04 6.5E-04 7.2E-04 6.9E-04 7.5E-04 8.9E-04 6.2E-04 9.1E-04 4.9E-04 Ribose 5-phosphate N.D. 2.6E-04 N.D. N.D. 1.9E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 2.1E-04 N.D. N.D. N.D. 242.080400555556_7.32524373333333 7.8E-04 9.1E-04 7.3E-04 7.9E-04 8.6E-04 7.8E-04 7.0E-04 6.8E-04 8.5E-04 6.4E-04 1.2E-03 1.0E-03 1.0E-03 8.4E-04 7.4E-04 6.8E-04 1.1E-03 9.4E-04 Fructose 6-phosphate 3.7E-02 3.1E-02 5.2E-02 3.6E-02 7.2E-02 8.0E-02 5.2E-02 9.0E-02 7.1E-02 8.3E-02 4.9E-02 3.7E-02 6.2E-02 9.3E-02 4.2E-02 8.3E-02 9.6E-02 8.9E-02 Glucose 6-phosphate 2.2E-01 1.1E-01 2.1E-01 1.3E-01 2.7E-01 2.8E-01 2.0E-01 3.8E-01 2.6E-01 3.0E-01 2.0E-01 1.4E-01 2.3E-01 3.8E-01 1.5E-01 3.1E-01 3.6E-01 3.6E-01 Glucose 1-phosphate 9.0E-03 5.8E-03 1.2E-02 7.4E-03 1.4E-02 1.5E-02 9.7E-03 1.9E-02 1.2E-02 1.5E-02 1.1E-02 7.0E-03 1.2E-02 2.1E-02 8.1E-03 1.6E-02 2.0E-02 1.6E-02 259.022887222222_9.88714338888889 2.8E-03 2.7E-03 1.6E-03 6.3E-04 1.1E-03 1.3E-03 1.5E-03 3.5E-03 1.6E-03 1.0E-03 1.1E-03 1.6E-03 1.3E-03 2.8E-03 2.2E-03 9.6E-04 1.4E-03 9.9E-04 2,3-Diphosphoglyceric acid N.D. 4.0E-04 N.D. N.D. N.D. N.D. 4.9E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 3.0E-04 N.D. 6-Phosphogluconic acid 2.2E-04 2.9E-04 2.4E-04 2.0E-04 2.4E-04 3.2E-04 4.2E-04 4.2E-04 3.2E-04 2.0E-04 2.4E-04 2.3E-04 3.1E-04 2.4E-04 3.2E-04 2.4E-04 2.8E-04 2.2E-04 N-Acetyl-alpha-neuraminic acid 4.5E-04 6.0E-04 4.6E-04 6.3E-04 3.9E-04 6.0E-04 5.8E-04 4.8E-04 4.2E-04 3.6E-04 3.6E-04 3.8E-04 3.6E-04 4.2E-04 3.5E-04 4.2E-04 4.5E-04 4.6E-04 Fructose 1,6-bisphosphate 9.8E-03 3.7E-02 3.1E-03 1.7E-02 1.5E-02 1.4E-02 4.2E-02 1.1E-01 4.3E-03 7.8E-03 1.2E-01 7.0E-03 2.6E-03 9.9E-03 1.3E-02 1.9E-02 3.2E-03 1.8E-02 AMP 2.4E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. 3.2E-04 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. IMP 3.4E-03 1.1E-02 1.2E-03 9.3E-03 4.7E-03 1.1E-02 5.1E-03 1.1E-02 2.0E-03 8.3E-03 2.4E-02 2.8E-03 1.4E-03 1.2E-02 2.3E-02 3.8E-03 3.8E-03 7.0E-03 Coenzyme A 3.6E-04 4.6E-04 4.7E-04 7.1E-04 2.5E-04 3.4E-04 5.6E-04 3.7E-04 2.6E-04 1.7E-04 3.2E-04 2.4E-04 2.9E-04 3.3E-04 3.5E-04 3.2E-04 3.5E-04 3.7E-04 Phosphoribosyl pyrophosphate 4.4E-04 4.6E-04 3.8E-04 4.3E-04 3.2E-04 1.8E-04 9.2E-04 2.8E-04 2.8E-04 2.9E-04 3.3E-04 3.4E-04 2.4E-04 3.3E-04 2.6E-04 2.1E-04 2.8E-04 1.5E-04 ADP 6.1E-03 2.9E-03 4.1E-03 3.4E-03 3.7E-03 2.6E-03 2.6E-03 3.6E-03 1.0E-02 6.4E-03 2.7E-03 4.1E-03 4.8E-03 3.5E-03 1.5E-03 4.6E-03 6.5E-03 9.3E-03 CTP 1.3E-03 1.1E-03 1.2E-03 1.2E-03 1.1E-03 1.2E-03 1.3E-03 1.7E-03 1.1E-03 8.4E-04 1.1E-03 1.0E-03 1.1E-03 1.4E-03 1.1E-03 1.3E-03 1.2E-03 1.2E-03 UTP 4.0E-03 4.3E-03 4.2E-03 4.4E-03 4.0E-03 3.7E-03 4.7E-03 3.2E-03 3.7E-03 3.5E-03 4.1E-03 3.6E-03 4.3E-03 4.0E-03 3.8E-03 3.8E-03 5.3E-03 4.4E-03 ATP 4.4E-01 4.3E-01 4.3E-01 4.1E-01 4.2E-01 4.0E-01 4.4E-01 4.4E-01 4.1E-01 4.3E-01 3.9E-01 3.6E-01 4.1E-01 4.6E-01 3.6E-01 4.5E-01 4.8E-01 4.1E-01 GTP 4.5E-03 4.7E-03 3.6E-03 4.1E-03 3.7E-03 3.4E-03 4.1E-03 3.8E-03 3.2E-03 3.2E-03 2.9E-03 3.4E-03 3.6E-03 3.7E-03 2.9E-03 3.2E-03 4.5E-03 3.6E-03 ADP-ribose 1.9E-03 1.3E-03 1.2E-03 1.6E-03 2.3E-03 8.6E-04 3.0E-03 1.6E-03 2.1E-03 1.2E-03 2.4E-03 2.2E-03 1.9E-03 1.9E-03 3.2E-03 1.1E-03 3.1E-03 1.8E-03 565.048853333333_8.27918502777778 1.1E-03 1.0E-03 8.9E-04 1.1E-03 8.4E-04 8.8E-04 8.1E-04 1.0E-03 7.0E-04 7.4E-04 9.3E-04 1.0E-03 7.9E-04 8.0E-04 6.7E-04 5.3E-04 6.5E-04 8.0E-04 GDP-glucose 3.4E-04 2.4E-04 2.3E-04 3.1E-04 2.0E-04 3.8E-04 2.9E-04 2.6E-04 2.7E-04 2.1E-04 1.8E-04 2.4E-04 2.1E-04 2.1E-04 2.5E-04 1.8E-04 2.2E-04 2.1E-04 UDP-N-acetylglucosamine 8.4E-04 1.3E-03 8.8E-04 7.6E-04 1.1E-03 1.1E-03 9.2E-04 9.7E-04 9.0E-04 1.2E-03 8.9E-04 1.1E-03 1.1E-03 8.5E-04 1.2E-03 9.6E-04 1.1E-03 1.2E-03 613.139846666667_7.84942670555556 2.5E-04 3.4E-04 2.0E-04 1.6E-04 2.5E-04 2.3E-04 2.0E-04 2.1E-04 2.6E-04 2.1E-04 2.1E-04 2.8E-04 2.4E-04 1.8E-04 2.9E-04 2.8E-04 2.5E-04 2.8E-04 NAD+ 1.7E-02 1.6E-02 1.9E-02 1.6E-02 1.6E-02 1.7E-02 1.3E-02 2.2E-02 1.4E-02 2.4E-02 1.8E-02 1.6E-02 1.9E-02 2.5E-02 1.6E-02 2.2E-02 2.1E-02 2.1E-02 NADH 1.4E-04 1.7E-04 1.8E-04 1.9E-04 1.9E-04 1.8E-04 1.5E-04 3.4E-04 2.0E-04 2.4E-04 4.0E-04 2.9E-04 3.5E-04 3.2E-04 5.1E-04 3.2E-04 4.5E-04 4.6E-04 NADP+ 2.5E-04 3.5E-04 3.1E-04 2.1E-04 2.0E-04 3.0E-04 4.0E-04 2.8E-04 1.7E-04 2.4E-04 2.2E-04 2.8E-04 2.3E-04 2.1E-04 1.6E-04 1.8E-04 2.2E-04 2.7E-04 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z MT HMDB id Pyruvic acid 87.00962056 12.40085781 HMDB00243 Lactic acid 89.02517383 10.46841919 HMDB00190,HMDB01311 3-Hydroxybutyric acid 103.0405737 9.164689139 HMDB00011,HMDB00357,HMDB00442 2-Hydroxyisobutyric acid 103.0406318 9.552653056 HMDB00729 Fumaric acid 115.0036454 24.56764133 HMDB00134 Succinic acid 117.0193965 20.24845467 HMDB00254 2-Hydroxyethanesulfonate 124.9919281 11.16497683 HMDB03903 Pyroglutamic acid 128.0353733 9.111180611 HMDB00267 129.055340909091_9.182088 129.0553409 9.182088 HMDB00695,HMDB00491 Glutaric acid 131.03488 16.152134 HMDB00661 Malic acid 133.013815 20.64892056 HMDB00156,HMDB00744 Phosphoethanolamine 140.0119594 7.689932972 HMDB00224 Caprylic acid 143.1070825 8.02209725 HMDB00482 Oxoglutaric acid 145.0136086 20.66533686 HMDB00208 Adipic acid 145.05028 14.394217 HMDB00448 2-Hydroxyglutaric acid 147.0295912 16.24756694 HMDB00606,HMDB00694 Pelargonic acid 157.1230778 7.814912878 HMDB00847 Terephthalic acid 165.0194406 16.03867328 HMDB02428 Phosphoenolpyruvic acid 166.97612 20.388746 HMDB00263 Uric acid 167.0205377 8.542642231 HMDB00289 Dihydroxyacetone phosphate 168.9911028 12.20518044 HMDB01473 Glyceraldehyde 3-phosphate 168.9911618 11.23064923 HMDB01112 sn-Glycero-3-phosphate 171.0063078 11.67935556 HMDB00126 N-Acetylaspartic acid 174.040505 14.05052136 HMDB00812 Vitamin C 175.02421 8.135187944 HMDB00044 Pyrophosphate 176.9354394 16.10719088 HMDB00250 3-Phosphoglyceric acid 184.9859989 18.99338406 HMDB00807 186.113085555556_7.54518425555555 186.1130856 7.545184256 No ID Azelaic acid 187.097398 11.707184 HMDB00784 191.018935_7.97564147777778 191.018935 7.975641478 No ID Citric acid 191.0194122 25.49059722 HMDB00094 Quinic acid 191.05731 7.9149155 HMDB03072 Phenylacetylglycine 192.067215 7.8808205 HMDB00821 Galacturonic acid 193.0355233 8.023212333 HMDB02545 Gluconic acid 195.0504713 7.829421875 HMDB00625 Lauric acid 199.170458 7.35806062 HMDB00638 Phosphocreatine 210.0283506 11.84327119 HMDB01511 Indoxyl sulfate 212.00328 9.189546 HMDB00682 Pantothenic acid 218.1034328 7.365927672 HMDB00210 Ribulose 5-phosphate 229.0124422 10.57174589 HMDB00618 Ribose 5-phosphate 229.0127333 10.1605225 HMDB01548 242.080400555556_7.32524373333333 242.0804006 7.325243733 No ID Fructose 6-phosphate 259.0228161 9.604942833 HMDB00124 Glucose 6-phosphate 259.0228233 9.52006375 HMDB01401 Glucose 1-phosphate 259.0228422 9.761735389 HMDB01586 259.022887222222_9.88714338888889 259.0228872 9.887143389 HMDB00213,HMDB06814 2,3-Diphosphoglyceric acid 264.95087 17.889198 HMDB01294 6-Phosphogluconic acid 275.0176461 14.16077414 HMDB01316 N-Acetyl-alpha-neuraminic acid 308.0995044 6.92161085 HMDB00230 Fructose 1,6-bisphosphate 338.9894006 14.05784978 HMDB01058 AMP 346.05702 8.8899555 HMDB00045 IMP 347.0407572 9.156668528 HMDB00175 Coenzyme A 382.5526872 10.24162994 HMDB01423 Phosphoribosyl pyrophosphate 388.9465278 16.08772617 HMDB00280 ADP 426.0236133 10.48380131 HMDB01341 CTP 481.9790594 11.89356044 HMDB00082 UTP 482.9629161 12.06716389 HMDB00285 ATP 505.9899322 11.37430011 HMDB00538 GTP 521.9848756 11.01270536 HMDB01273 ADP-ribose 558.0646244 8.148109056 HMDB01178 565.048853333333_8.27918502777778 565.0488533 8.279185028 HMDB00286,HMDB00302 GDP-glucose 604.0706589 7.952294417 HMDB03351 UDP-N-acetylglucosamine 606.0748467 8.095755222 HMDB00290 613.139846666667_7.84942670555556 613.1398467 7.849426706 HMDB01176 NAD+ 662.1029339 6.318070156 HMDB00902 NADH 664.1169556 7.851938289 HMDB01487 NADP+ 742.0688033 9.002893056 HMDB00217 METABOLITES_END #END