#METABOLOMICS WORKBENCH sven_haange_20210615_230533_mwtab.txt DATATRACK_ID:2693 STUDY_ID:ST001832 ANALYSIS_ID:AN002974 PROJECT_ID:PR001157 VERSION 1 CREATED_ON June 17, 2021, 5:53 pm #PROJECT PR:PROJECT_TITLE Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced PR:PROJECT_TITLE obesity rat model PR:PROJECT_SUMMARY Investigating alterations in the intestinal microbiome metabolome and host blood PR:PROJECT_SUMMARY metabolome in a diet induced obesity (DIO) rat model after fecal transplant from PR:PROJECT_SUMMARY rats, which underwent Roux-Y-Gastric-Bypass surgery (RYGB). Experimental groups PR:PROJECT_SUMMARY for rat experiments. 1)Rats in the ‘RYGB_Donor group’ underwent RYGB PR:PROJECT_SUMMARY surgery. Postoperatively, animals received a two-choice diet, consisting of a SC PR:PROJECT_SUMMARY and HFD. Once the animals recovered and achieved a stabilized weight reduction PR:PROJECT_SUMMARY at 4 weeks postoperatively, body weight and food intake were recorded daily. PR:PROJECT_SUMMARY 2)Rats allocated into the ‘RYGB_AB group’ were handled the same as animals PR:PROJECT_SUMMARY in the ‘RYGB_donor group’. From postoperative week 4 onwards, animals PR:PROJECT_SUMMARY received an antibiotic treatment (AB) consisting of ampicillin (1 g/l; PR:PROJECT_SUMMARY Ratiopharm), vancomycin (0.5 g/l; Ratiopharm), neomycin (1 g/l; Bela-pharm), and PR:PROJECT_SUMMARY metronidazole (1 g/l; CP-Pharma), provided freshly every day via drinking PR:PROJECT_SUMMARY water.[19, 20] All antibiotics were given for the time period indicated in each PR:PROJECT_SUMMARY figure.[21] 3)Rats in the ‘Lean group’ served as healthy, age-matched PR:PROJECT_SUMMARY controls, constantly kept under SC. 4)Rats in the ‘Lean_AB group’ received PR:PROJECT_SUMMARY the same antibiotic treatment as animals in the RYGB_AB group, serving as a PR:PROJECT_SUMMARY control for antibiotic-specific side effects. All antibiotics were given for the PR:PROJECT_SUMMARY time periods indicated in each figure. 5)Rats in the ‘DIO group’ received no PR:PROJECT_SUMMARY surgery, but were otherwise handled like RYGB litter mates. 6)Rats in the PR:PROJECT_SUMMARY ‘DIO_FMT_RYGB group’ received fecal RYGB_Donor microbiota transplantation PR:PROJECT_SUMMARY once per week, but were otherwise handled like DIO litter mates. For fecal PR:PROJECT_SUMMARY transplantation experiments, 100 mg of fresh stool from body weight-stabilized PR:PROJECT_SUMMARY RYGB donors were re-suspended in 1 ml of PBS, homogenized carefully and PR:PROJECT_SUMMARY administered via oral gavage with 200 μl of the suspension. 7)Rats in the PR:PROJECT_SUMMARY ‘FMT_Lean group’ were handled like DIO litter mates, but were orally gavaged PR:PROJECT_SUMMARY with 200 μl of filtrate received from lean donors, and served as a control for PR:PROJECT_SUMMARY fecal transplantation and specificity of effect size(s) as a function of the PR:PROJECT_SUMMARY donor microbiota. 8)Rats in the ‘DIO_PF_FMT group’ were handled like DIO PR:PROJECT_SUMMARY litter mates, but pair-fed to the SC/HFD food intake of ‘FMT_Lean littermates, PR:PROJECT_SUMMARY serving as a control for effects secondary to reduced caloric intake. PR:INSTITUTE Helmholtz Centre for Environmental Research - UFZ PR:LAST_NAME Haange PR:FIRST_NAME Sven PR:ADDRESS Permoserstrasse 1, Leipzig, Saxony, 04318, Germany PR:EMAIL sven.haange@ufz.de PR:PHONE 0049 341 2351099 #STUDY ST:STUDY_TITLE Quantitative bile acids study on blood serum and ceacal content of rat models ST:STUDY_TITLE (part I) ST:STUDY_SUMMARY Bile acids were analyzed in blood serum and cecal content in rat models ST:INSTITUTE Helmholtz Centre for Environmental Research - UFZ ST:DEPARTMENT Department of Molecular Systems biology ST:LABORATORY Functional Metabolomics ST:LAST_NAME Rolle-Kampczyk ST:FIRST_NAME Ulrike ST:ADDRESS Permoserstrasse 15, 04318 Leipzig, Germany ST:EMAIL ulrike.rolle-kampczyk@ufz.de ST:STUDY_TYPE blood serum and caecum content ST:PHONE 0049 341 235 1537 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 1024040931 Tissue:Caecum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_56_0_1_1_00_1024040931.wiff SUBJECT_SAMPLE_FACTORS - 1024039050 Tissue:Caecum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_18_0_1_1_00_1024039050.wiff SUBJECT_SAMPLE_FACTORS - 1024041499 Tissue:Caecum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_51_0_1_1_00_1024041499.wiff SUBJECT_SAMPLE_FACTORS - 1024040999 Tissue:Caecum | Rat_number:7 | Rat_sample_name:DIO_2 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_33_0_1_1_00_1024040999.wiff SUBJECT_SAMPLE_FACTORS - 1024041015 Tissue:Caecum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_57_0_1_1_00_1024041015.wiff SUBJECT_SAMPLE_FACTORS - 1024041020 Tissue:Caecum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_69_0_1_1_00_1024041020.wiff SUBJECT_SAMPLE_FACTORS - 1024039045 Tissue:Caecum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_06_0_1_1_00_1024039045.wiff SUBJECT_SAMPLE_FACTORS - 1024041673 Tissue:Caecum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_77_0_1_1_00_1024041673.wiff SUBJECT_SAMPLE_FACTORS - 1024039031 Tissue:Caecum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_89_0_1_1_00_1024039031.wiff SUBJECT_SAMPLE_FACTORS - 1024040946 Tissue:Caecum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_68_0_1_1_00_1024040946.wiff SUBJECT_SAMPLE_FACTORS - 1024040951 Tissue:Caecum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_80_0_1_1_00_1024040951.wiff SUBJECT_SAMPLE_FACTORS - 1024040965 Tissue:Caecum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_92_0_1_1_00_1024040965.wiff SUBJECT_SAMPLE_FACTORS - 1024040984 Tissue:Caecum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_21_0_1_1_00_1024040984.wiff SUBJECT_SAMPLE_FACTORS - 1024035907 Tissue:Caecum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5512_1024036014_62_0_1_1_00_1024035907.wiff SUBJECT_SAMPLE_FACTORS - 1024038632 Tissue:Caecum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_96_0_1_1_00_1024038632.wiff SUBJECT_SAMPLE_FACTORS - 1024040970 Tissue:Caecum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_09_0_1_1_00_1024040970.wiff SUBJECT_SAMPLE_FACTORS - 1024041635 Tissue:Caecum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_29_0_1_1_00_1024041635.wiff SUBJECT_SAMPLE_FACTORS - 1024041669 Tissue:Caecum | Rat_number:131 | Rat_sample_name:DIO_FMT_RYGB_12 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_65_0_1_1_00_1024041669.wiff SUBJECT_SAMPLE_FACTORS - 1024041654 Tissue:Caecum | Rat_number:130 | Rat_sample_name:DIO_FMT_RYGB_11 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_53_0_1_1_00_1024041654.wiff SUBJECT_SAMPLE_FACTORS - 1024041001 Tissue:Caecum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_45_0_1_1_00_1024041001.wiff SUBJECT_SAMPLE_FACTORS - 1024041034 Tissue:Caecum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_81_0_1_1_00_1024041034.wiff SUBJECT_SAMPLE_FACTORS - 1024041049 Tissue:Caecum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_93_0_1_1_00_1024041049.wiff SUBJECT_SAMPLE_FACTORS - 1024041514 Tissue:Caecum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_75_0_1_1_00_1024041514.wiff SUBJECT_SAMPLE_FACTORS - 1024035931 Tissue:Caecum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_03_0_1_1_00_1024035931.wiff SUBJECT_SAMPLE_FACTORS - 1024041640 Tissue:Caecum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_41_0_1_1_00_1024041640.wiff SUBJECT_SAMPLE_FACTORS - 1024039083 Tissue:Caecum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_54_0_1_1_00_1024039083.wiff SUBJECT_SAMPLE_FACTORS - 1024039109 Tissue:Caecum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_78_0_1_1_00_1024039109.wiff SUBJECT_SAMPLE_FACTORS - 1024039113 Tissue:Caecum | Rat_number:135 | Rat_sample_name:DIO_PF_FMT_3 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_90_0_1_1_00_1024039113.wiff SUBJECT_SAMPLE_FACTORS - 1024039098 Tissue:Caecum | Rat_number:133 | Rat_sample_name:DIO_PF_FMT_4 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_66_0_1_1_00_1024039098.wiff SUBJECT_SAMPLE_FACTORS - 1024039079 Tissue:Caecum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_42_0_1_1_00_1024039079.wiff SUBJECT_SAMPLE_FACTORS - 1024035896 Tissue:Caecum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5512_1024036014_50_0_1_1_00_1024035896.wiff SUBJECT_SAMPLE_FACTORS - 1024035945 Tissue:Caecum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5512_1024036014_15_0_1_1_00_1024035945.wiff SUBJECT_SAMPLE_FACTORS - 1024035964 Tissue:Caecum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5512_1024036014_39_0_1_1_00_1024035964.wiff SUBJECT_SAMPLE_FACTORS - 1024041529 Tissue:Caecum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5512_1024036014_87_0_1_1_00_1024041529.wiff SUBJECT_SAMPLE_FACTORS - 1024041533 Tissue:Caecum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5512_1024036014_04_0_1_1_00_1024041533.wiff SUBJECT_SAMPLE_FACTORS - 1024038481 Tissue:Caecum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_11_0_1_1_00_1024038481.wiff SUBJECT_SAMPLE_FACTORS - 1024039151 Tissue:Caecum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_43_0_1_1_00_1024039151.wiff SUBJECT_SAMPLE_FACTORS - 1024038496 Tissue:Caecum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_23_0_1_1_00_1024038496.wiff SUBJECT_SAMPLE_FACTORS - 1024038526 Tissue:Caecum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_59_0_1_1_00_1024038526.wiff SUBJECT_SAMPLE_FACTORS - 1024038531 Tissue:Caecum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_71_0_1_1_00_1024038531.wiff SUBJECT_SAMPLE_FACTORS - 1024035926 Tissue:Caecum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_86_0_1_1_00_1024035926.wiff SUBJECT_SAMPLE_FACTORS - 1024039128 Tissue:Caecum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_07_0_1_1_00_1024039128.wiff SUBJECT_SAMPLE_FACTORS - 1024039132 Tissue:Caecum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_19_0_1_1_00_1024039132.wiff SUBJECT_SAMPLE_FACTORS - 1024039147 Tissue:Caecum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_31_0_1_1_00_1024039147.wiff SUBJECT_SAMPLE_FACTORS - 1024038462 Tissue:Caecum | Rat_number:27 | Rat_sample_name:Lean_AB_1 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_82_0_1_1_00_1024038462.wiff SUBJECT_SAMPLE_FACTORS - 1024038477 Tissue:Caecum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_94_0_1_1_00_1024038477.wiff SUBJECT_SAMPLE_FACTORS - 1024038507 Tissue:Caecum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_35_0_1_1_00_1024038507.wiff SUBJECT_SAMPLE_FACTORS - 1024038511 Tissue:Caecum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_47_0_1_1_00_1024038511.wiff SUBJECT_SAMPLE_FACTORS - 1024038613 Tissue:Caecum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_72_0_1_1_00_1024038613.wiff SUBJECT_SAMPLE_FACTORS - 1024038628 Tissue:Caecum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_84_0_1_1_00_1024038628.wiff SUBJECT_SAMPLE_FACTORS - 1024041072 Tissue:Caecum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_34_0_1_1_00_1024041072.wiff SUBJECT_SAMPLE_FACTORS - 1024041053 Tissue:Caecum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_10_0_1_1_00_1024041053.wiff SUBJECT_SAMPLE_FACTORS - 1024041102 Tissue:Caecum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_70_0_1_1_00_1024041102.wiff SUBJECT_SAMPLE_FACTORS - 1024038579 Tissue:Caecum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_24_0_1_1_00_1024038579.wiff SUBJECT_SAMPLE_FACTORS - 1024038583 Tissue:Caecum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_36_0_1_1_00_1024038583.wiff SUBJECT_SAMPLE_FACTORS - 1024038609 Tissue:Caecum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_60_0_1_1_00_1024038609.wiff SUBJECT_SAMPLE_FACTORS - 1024041591 Tissue:Caecum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5512_1024036014_76_0_1_1_00_1024041591.wiff SUBJECT_SAMPLE_FACTORS - 1024041621 Tissue:Caecum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5512_1024036014_17_0_1_1_00_1024041621.wiff SUBJECT_SAMPLE_FACTORS - 1024041586 Tissue:Caecum | Rat_number:111 | Rat_sample_name:RYGB_AB_9 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5512_1024036014_64_0_1_1_00_1024041586.wiff SUBJECT_SAMPLE_FACTORS - 1024041571 Tissue:Caecum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_52_0_1_1_00_1024041571.wiff SUBJECT_SAMPLE_FACTORS - 1024041087 Tissue:Caecum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_46_0_1_1_00_1024041087.wiff SUBJECT_SAMPLE_FACTORS - 1024041091 Tissue:Caecum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_58_0_1_1_00_1024041091.wiff SUBJECT_SAMPLE_FACTORS - 1024038545 Tissue:Caecum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_83_0_1_1_00_1024038545.wiff SUBJECT_SAMPLE_FACTORS - 1024038564 Tissue:Caecum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_12_0_1_1_00_1024038564.wiff SUBJECT_SAMPLE_FACTORS - 1024038550 Tissue:Caecum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_95_0_1_1_00_1024038550.wiff SUBJECT_SAMPLE_FACTORS - 1024041552 Tissue:Caecum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_28_0_1_1_00_1024041552.wiff SUBJECT_SAMPLE_FACTORS - 1024041567 Tissue:Caecum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_40_0_1_1_00_1024041567.wiff SUBJECT_SAMPLE_FACTORS - 1024041548 Tissue:Caecum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_16_0_1_1_00_1024041548.wiff SUBJECT_SAMPLE_FACTORS - 1024038375 Tissue:Serum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_50_0_1_1_00_1024038375.wiff SUBJECT_SAMPLE_FACTORS - 1024033225 Tissue:Serum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_57_0_1_1_00_1024033225.wiff SUBJECT_SAMPLE_FACTORS - 1024035363 Tissue:Serum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_91_0_1_1_00_1024035363.wiff SUBJECT_SAMPLE_FACTORS - 1024035378 Tissue:Serum | Rat_number:70 | Rat_sample_name:DIO_12 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_08_0_1_1_00_1024035378.wiff SUBJECT_SAMPLE_FACTORS - 1024038443 Tissue:Serum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_39_0_1_1_00_1024038443.wiff SUBJECT_SAMPLE_FACTORS - 1024038458 Tissue:Serum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5502_1024038647_51_0_1_1_00_1024038458.wiff SUBJECT_SAMPLE_FACTORS - 1024033211 Tissue:Serum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_45_0_1_1_00_1024033211.wiff SUBJECT_SAMPLE_FACTORS - 1024033195 Tissue:Serum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_21_0_1_1_00_1024033195.wiff SUBJECT_SAMPLE_FACTORS - 1024033206 Tissue:Serum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO RAW_FILE_NAME=BA02-0-5512_1024036014_33_0_1_1_00_1024033206.wiff SUBJECT_SAMPLE_FACTORS - 1024038380 Tissue:Serum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_62_0_1_1_00_1024038380.wiff SUBJECT_SAMPLE_FACTORS - 1024038394 Tissue:Serum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_74_0_1_1_00_1024038394.wiff SUBJECT_SAMPLE_FACTORS - 1024038405 Tissue:Serum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_86_0_1_1_00_1024038405.wiff SUBJECT_SAMPLE_FACTORS - 1024038424 Tissue:Serum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_15_0_1_1_00_1024038424.wiff SUBJECT_SAMPLE_FACTORS - 1024035281 Tissue:Serum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_90_0_1_1_00_1024035281.wiff SUBJECT_SAMPLE_FACTORS - 1024035261 Tissue:Serum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB RAW_FILE_NAME=BA02-0-5502_1024038647_66_0_1_1_00_1024035261.wiff SUBJECT_SAMPLE_FACTORS - 1024038410 Tissue:Serum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_03_0_1_1_00_1024038410.wiff SUBJECT_SAMPLE_FACTORS - 1024033157 Tissue:Serum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_68_0_1_1_00_1024033157.wiff SUBJECT_SAMPLE_FACTORS - 1024038439 Tissue:Serum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_27_0_1_1_00_1024038439.wiff SUBJECT_SAMPLE_FACTORS - 1024032366 Tissue:Serum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_63_0_1_1_00_1024032366.wiff SUBJECT_SAMPLE_FACTORS - 1024032371 Tissue:Serum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_75_0_1_1_00_1024032371.wiff SUBJECT_SAMPLE_FACTORS - 1024035382 Tissue:Serum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_20_0_1_1_00_1024035382.wiff SUBJECT_SAMPLE_FACTORS - 1024035311 Tissue:Serum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5502_1024038647_31_0_1_1_00_1024035311.wiff SUBJECT_SAMPLE_FACTORS - 1024033161 Tissue:Serum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB RAW_FILE_NAME=BA02-0-5512_1024036014_80_0_1_1_00_1024033161.wiff SUBJECT_SAMPLE_FACTORS - 1024033259 Tissue:Serum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_93_0_1_1_00_1024033259.wiff SUBJECT_SAMPLE_FACTORS - 1024033282 Tissue:Serum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_34_0_1_1_00_1024033282.wiff SUBJECT_SAMPLE_FACTORS - 1024033230 Tissue:Serum | Rat_number:124 | Rat_sample_name:DIO_PF_FMT_5 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_69_0_1_1_00_1024033230.wiff SUBJECT_SAMPLE_FACTORS - 1024033244 Tissue:Serum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT RAW_FILE_NAME=BA02-0-5512_1024036014_81_0_1_1_00_1024033244.wiff SUBJECT_SAMPLE_FACTORS - 1024035276 Tissue:Serum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_78_0_1_1_00_1024035276.wiff SUBJECT_SAMPLE_FACTORS - 1024035325 Tissue:Serum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_43_0_1_1_00_1024035325.wiff SUBJECT_SAMPLE_FACTORS - 1024035330 Tissue:Serum | Rat_number:63 | Rat_sample_name:FMT_lean_3 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_55_0_1_1_00_1024035330.wiff SUBJECT_SAMPLE_FACTORS - 1024035359 Tissue:Serum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_79_0_1_1_00_1024035359.wiff SUBJECT_SAMPLE_FACTORS - 1024035397 Tissue:Serum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_32_0_1_1_00_1024035397.wiff SUBJECT_SAMPLE_FACTORS - 1024040927 Tissue:Serum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean RAW_FILE_NAME=BA02-0-5502_1024038647_44_0_1_1_00_1024040927.wiff SUBJECT_SAMPLE_FACTORS - 1024032453 Tissue:Serum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_76_0_1_1_00_1024032453.wiff SUBJECT_SAMPLE_FACTORS - 1024035998 Tissue:Serum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_94_0_1_1_00_1024035998.wiff SUBJECT_SAMPLE_FACTORS - 1024032468 Tissue:Serum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_88_0_1_1_00_1024032468.wiff SUBJECT_SAMPLE_FACTORS - 1024032502 Tissue:Serum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_41_0_1_1_00_1024032502.wiff SUBJECT_SAMPLE_FACTORS - 1024032517 Tissue:Serum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_53_0_1_1_00_1024032517.wiff SUBJECT_SAMPLE_FACTORS - 1024035295 Tissue:Serum | Rat_number:54 | Rat_sample_name:Lean_5 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_07_0_1_1_00_1024035295.wiff SUBJECT_SAMPLE_FACTORS - 1024035306 Tissue:Serum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5502_1024038647_19_0_1_1_00_1024035306.wiff SUBJECT_SAMPLE_FACTORS - 1024033308 Tissue:Serum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_58_0_1_1_00_1024033308.wiff SUBJECT_SAMPLE_FACTORS - 1024035979 Tissue:Serum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_70_0_1_1_00_1024035979.wiff SUBJECT_SAMPLE_FACTORS - 1024035983 Tissue:Serum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean RAW_FILE_NAME=BA02-0-5512_1024036014_82_0_1_1_00_1024035983.wiff SUBJECT_SAMPLE_FACTORS - 1024032449 Tissue:Serum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_64_0_1_1_00_1024032449.wiff SUBJECT_SAMPLE_FACTORS - 1024032487 Tissue:Serum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_17_0_1_1_00_1024032487.wiff SUBJECT_SAMPLE_FACTORS - 1024032491 Tissue:Serum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_29_0_1_1_00_1024032491.wiff SUBJECT_SAMPLE_FACTORS - 1024035242 Tissue:Serum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_42_0_1_1_00_1024035242.wiff SUBJECT_SAMPLE_FACTORS - 1024035257 Tissue:Serum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB RAW_FILE_NAME=BA02-0-5502_1024038647_54_0_1_1_00_1024035257.wiff SUBJECT_SAMPLE_FACTORS - 1024032390 Tissue:Serum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_04_0_1_1_00_1024032390.wiff SUBJECT_SAMPLE_FACTORS - 1024032385 Tissue:Serum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_87_0_1_1_00_1024032385.wiff SUBJECT_SAMPLE_FACTORS - 1024032434 Tissue:Serum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_52_0_1_1_00_1024032434.wiff SUBJECT_SAMPLE_FACTORS - 1024035219 Tissue:Serum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_06_0_1_1_00_1024035219.wiff SUBJECT_SAMPLE_FACTORS - 1024035223 Tissue:Serum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_18_0_1_1_00_1024035223.wiff SUBJECT_SAMPLE_FACTORS - 1024035238 Tissue:Serum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5502_1024038647_30_0_1_1_00_1024035238.wiff SUBJECT_SAMPLE_FACTORS - 1024033123 Tissue:Serum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5512_1024036014_32_0_1_1_00_1024033123.wiff SUBJECT_SAMPLE_FACTORS - 1024033142 Tissue:Serum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB RAW_FILE_NAME=BA02-0-5512_1024036014_56_0_1_1_00_1024033142.wiff SUBJECT_SAMPLE_FACTORS - 1024032415 Tissue:Serum | Rat_number:18b | Rat_sample_name:RYGB_Donor_1 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_28_0_1_1_00_1024032415.wiff SUBJECT_SAMPLE_FACTORS - 1024039201 Tissue:Serum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_08_0_1_1_00_1024039201.wiff SUBJECT_SAMPLE_FACTORS - 1024032401 Tissue:Serum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_16_0_1_1_00_1024032401.wiff SUBJECT_SAMPLE_FACTORS - 1024032420 Tissue:Serum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_40_0_1_1_00_1024032420.wiff SUBJECT_SAMPLE_FACTORS - 1024032521 Tissue:Serum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_65_0_1_1_00_1024032521.wiff SUBJECT_SAMPLE_FACTORS - 1024032541 Tissue:Serum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_89_0_1_1_00_1024032541.wiff SUBJECT_SAMPLE_FACTORS - 1024032536 Tissue:Serum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5502_1024038647_77_0_1_1_00_1024032536.wiff SUBJECT_SAMPLE_FACTORS - 1024039185 Tissue:Serum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_79_0_1_1_00_1024039185.wiff SUBJECT_SAMPLE_FACTORS - 1024039190 Tissue:Serum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_91_0_1_1_00_1024039190.wiff SUBJECT_SAMPLE_FACTORS - 1024039166 Tissue:Serum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor RAW_FILE_NAME=BA02-0-5512_1024036014_55_0_1_1_00_1024039166.wiff #COLLECTION CO:COLLECTION_SUMMARY Animals were sacrificed, blood and cecum tissue were removed, snap frozen in CO:COLLECTION_SUMMARY liquid nitrogen and stored at -80°C until further analysis CO:SAMPLE_TYPE Cecum #TREATMENT TR:TREATMENT_SUMMARY RYGB surgery was performed on age-matched (8-10-weeks old) male Wistar IGS rats. TR:TREATMENT_SUMMARY After four weeks of recovery, animals were put on an antibiotic cocktail TR:TREATMENT_SUMMARY consisting of ampicillin (1 g/l; Ratiopharm), vancomycin (0.5 g/l; Ratiopharm), TR:TREATMENT_SUMMARY neomycin (1 g/l; Bela-pharm), and metronidazole (1 g/l; CP-Pharma), provided TR:TREATMENT_SUMMARY freshly every day via drinking water. The parallel FMT group received fecal RYGB TR:TREATMENT_SUMMARY microbiota transplantation once per week, but were otherwise handled like DIO TR:TREATMENT_SUMMARY littermates. For fecal transplantation experiments, 100 mg of fresh stool from TR:TREATMENT_SUMMARY body weight-stabilized RYGB donors was re-suspended in 1 ml of PBS, homogenized TR:TREATMENT_SUMMARY carefully and administered via oral gavage with 200 μl of the suspension. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) SP:SAMPLEPREP_SUMMARY Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M SP:SAMPLEPREP_SUMMARY Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work SP:SAMPLEPREP_SUMMARY steps To approximately 100 mg of sample four steel balls of size M rae added in SP:SAMPLEPREP_SUMMARY Eppendorf tubes. Then add for each mg of sample 5 µL of extracting agent. Shake SP:SAMPLEPREP_SUMMARY the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 SP:SAMPLEPREP_SUMMARY minutes at 14000 rpm. The supernatant is used for targeted analytics as SP:SAMPLEPREP_SUMMARY described in the kit manufactures manual. Kit measurement The analysis was SP:SAMPLEPREP_SUMMARY performed using the validated Bile Acid Kit (Biocrates Life Sciences, Inssbruck, SP:SAMPLEPREP_SUMMARY Austria) as described in Pham et al. 2017 [1]. Data processing is carried out SP:SAMPLEPREP_SUMMARY with the provided quantitation method Kit (Biocrates Life Sciences AG, SP:SAMPLEPREP_SUMMARY Innsbruck, Austria). 1 Pham HT, Arnhard K, Asad YJ, Deng L, Felder TK, St SP:SAMPLEPREP_SUMMARY John-Williams L, et al. Inter-Laboratory Robustness of Next-Generation Bile Acid SP:SAMPLEPREP_SUMMARY Study in Mice and Humans: International Ring Trial Involving 12 Laboratories. SP:SAMPLEPREP_SUMMARY The Journal of Applied Laboratory Medicine 2019;1:129-42. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME UPLC (Waters Acquity, Waters Corporation, Milford, USA) CH:COLUMN_NAME none #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version MS:MS_COMMENTS Boron from Biocrates #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS µM MS_METABOLITE_DATA_START Samples 1024038410 1024032390 1024035219 1024035295 1024035378 1024040970 1024041053 1024038481 1024038564 1024038424 1024032487 1024032401 1024035306 1024035223 1024040984 1024035382 1024038496 1024038579 1024038439 1024032491 1024032415 1024035311 1024035238 1024041072 1024038507 1024035397 1024040999 1024038443 1024038583 1024035242 1024035325 1024032420 1024032502 1024041087 1024038511 1024040927 1024041001 1024038458 1024038375 1024035330 1024035257 1024032517 1024032434 1024038526 1024041091 1024041015 1024040931 1024032366 1024038380 1024038609 1024040946 1024041020 1024041102 1024038531 1024032449 1024032521 1024035261 1024032453 1024032536 1024035276 1024035359 1024038613 1024038394 1024032371 1024038628 1024032385 1024038405 1024041034 1024040951 1024038545 1024038462 1024041049 1024040965 1024038550 1024038477 1024032541 1024032468 1024035363 1024035281 1024038632 1024039147 1024033282 1024033161 1024039185 1024033230 1024035931 1024033157 1024035896 1024041499 1024041533 1024041654 1024035979 1024041571 1024039050 1024039098 1024041529 1024039132 1024033195 1024033142 1024035964 1024041567 1024039128 1024033225 1024041640 1024033308 1024035926 1024039083 1024039045 1024039166 1024039201 1024041635 1024033211 1024041514 1024035998 1024033259 1024041552 1024041673 1024041591 1024039190 1024039079 1024039113 1024039151 1024039031 1024039109 1024035983 1024033123 1024033244 1024041548 1024041669 1024041621 1024033206 1024041586 1024035945 1024035907 Factors Tissue:Serum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:54 | Rat_sample_name:Lean_5 | Sample treatment:Lean Tissue:Serum | Rat_number:70 | Rat_sample_name:DIO_12 | Sample treatment:DIO Tissue:Caecum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean Tissue:Caecum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB Tissue:Serum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean Tissue:Serum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB Tissue:Serum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean Tissue:Caecum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:18b | Rat_sample_name:RYGB_Donor_1 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:7 | Rat_sample_name:DIO_2 | Sample treatment:DIO Tissue:Serum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO Tissue:Caecum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean Tissue:Serum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean Tissue:Caecum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO Tissue:Serum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO Tissue:Serum | Rat_number:63 | Rat_sample_name:FMT_lean_3 | Sample treatment:FMT_lean Tissue:Serum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean Tissue:Serum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean Tissue:Caecum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO Tissue:Caecum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO Tissue:Serum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO Tissue:Caecum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean Tissue:Serum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB Tissue:Serum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean Tissue:Serum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean Tissue:Serum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB Tissue:Serum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:27 | Rat_sample_name:Lean_AB_1 | Sample treatment:Lean_AB Tissue:Caecum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB Tissue:Serum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean Tissue:Serum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO Tissue:Serum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB Tissue:Caecum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean Tissue:Serum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT Tissue:Serum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:124 | Rat_sample_name:DIO_PF_FMT_5 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO Tissue:Caecum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:130 | Rat_sample_name:DIO_FMT_RYGB_11 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean Tissue:Caecum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO Tissue:Caecum | Rat_number:133 | Rat_sample_name:DIO_PF_FMT_4 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean Tissue:Serum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO Tissue:Serum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean Tissue:Serum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO Tissue:Caecum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean Tissue:Caecum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean Tissue:Caecum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO Tissue:Serum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor Tissue:Serum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO Tissue:Caecum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean Tissue:Serum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO Tissue:Caecum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:135 | Rat_sample_name:DIO_PF_FMT_3 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean Tissue:Caecum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO Tissue:Caecum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT Tissue:Serum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean Tissue:Serum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT Tissue:Caecum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor Tissue:Caecum | Rat_number:131 | Rat_sample_name:DIO_FMT_RYGB_12 | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB Tissue:Serum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO Tissue:Caecum | Rat_number:111 | Rat_sample_name:RYGB_AB_9 | Sample treatment:RYGB_AB Tissue:Caecum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean Tissue:Caecum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB Cholic Acid 0 0.126 0.755 4.44 0.801 1.08 20.6 1.77 0.303 0.028 0.015 3.48 4.39 0.026 17.4 0.243 2.97 42.9 0.335 0 0.185 0.214 0.472 10.8 0.704 0.735 0.197 0.513 2.42 0.302 0.513 7.09 1.84 0.115 1.12 2.69 1.76 0.021 1.26 0.196 0.005 0.591 0.096 0.209 0.463 0.816 2.01 0.187 0.093 10.9 12.2 3.06 8.19 0.208 0.006 10.6 0 2.01 3.85 0.108 2.35 21.3 0.004 2.38 10.6 4.04 0.115 7.3 20.2 12.1 0.334 8.33 17.9 4.75 0.492 4.54 1.87 1.39 0.46 7.11 0.249 3.2 6.27 20 1.8 5.01 0.356 2.93 16.1 29.9 3.76 2.88 0.438 5.69 7.48 2.2 0.293 5.28 0.077 1.17 0.56 0.328 0.25 2.52 0.183 0.823 16.2 4.12 0.093 1.11 9.27 1.6 9.4 4.76 0.072 0.399 1.31 25.4 3.42 1.56 13.1 1.1 1.56 1.7 1.48 0.083 1.47 NA 28.9 12.9 9.09 12.3 1.28 21.3 Chenodeoxycholic Acid 0.009 0.013 0.258 0.75 0.035 0 0.537 0 0 0.027 0.014 0.282 0.802 0.017 0.323 0.02 0 0 0.019 0.008 0.038 0.017 0.24 0.096 0 0.088 0 0.027 0 0.063 0.068 0.379 0.178 0 0 0.169 0 0.011 0.113 0.019 0.009 0.157 0.07 0.022 0 0 0 0.037 0.074 0.84 0.247 0 0.372 0 0.009 3.78 0.005 0.455 0.151 0.016 0.088 0.523 0.009 0.116 0.464 0.591 0.048 0 0.374 0.468 0 0.137 0.525 0 0 2.03 0.205 0.031 0.045 0.295 41.8 0.924 0.916 7.19 0.461 126 0.198 47.5 102 90.3 126 0.19 45.4 183 126 158 39.2 0.496 0 148 30.9 50.6 0.586 73.1 0.133 109 181 181 0 0.365 114 0.64 134 0.253 0.313 38.5 121 1.92 0.986 129 302 58.3 105 67.9 0.075 0 0.484 0 110 0 0.588 0 72.5 30.1 Deoxycholic Acid 0.061 0.007 0.638 0.113 0.08 NA 0.007 NA 1.13 0.029 0.058 0.136 0.231 0.013 NA 0.108 NA NA 0.064 0.06 0.007 0.069 0.01 0.007 3.23 0.125 NA 0.105 NA 0.01 0.032 0.097 0.025 0.353 2.59 0.065 NA 0.08 0.119 0.096 0.021 0.185 0.007 0.134 2.43 NA NA 0.169 0.112 0.018 NA NA 0.007 NA 0.053 0.113 0.184 0.263 0.11 0.07 0.172 0.668 0.009 0.147 7.69 0.094 0.007 NA 1.6 NA NA NA 0.008 NA 3.53 0.257 0.265 0.157 0.055 NA 11.5 0.343 0 2.09 0.171 27 0.09 13.1 24.3 21.4 28.9 0.098 12.3 37.4 28.5 30.7 11.3 0.176 0 31.7 8.81 14.3 0.235 30.1 0.067 25.1 37.2 37 0 0.16 25.2 0.234 30 0.111 0.135 11.1 25.5 0.685 0.36 29.3 51.2 15.7 24.7 31.3 0.048 0 0.189 0.327 41.8 0 0.229 0.058 18.4 9.03 Glycocholic Acid 0.101 0.288 2.21 0.225 0.176 0 1.42 0 0.002 0.068 0.015 8.29 0.073 0.449 0.082 1.2 0 0.084 0.247 0.014 1.37 0.102 4.31 0.502 0.002 1.19 0 0.183 0.012 0.101 0.371 3.59 0.337 0.023 0 1.11 0 0.075 0.114 0.135 0.013 0.117 0.249 0 0.005 0.013 0.002 0.235 0 0.094 0.197 0 0.029 0 0.029 8.23 0.284 0.047 0.909 0.196 0.283 0.064 0.014 0.634 0.051 4.77 0.054 0 0.081 0 0 0.061 0.255 0.173 0.001 2.91 0.161 0.233 0.146 0.532 0.025 1.26 1.76 4.79 0.677 0.022 0.324 0.001 0.043 0.08 0.054 1.28 0.04 0.046 0.017 0.08 0.054 0.567 0.119 0.022 0.043 0.025 0.473 0.061 1.13 0.035 0.098 0.042 0.215 1.15 0.07 0.319 0 0.146 0.11 0.106 0.119 0.025 0.398 0.177 0.06 0.019 0.089 0.188 0.201 0.039 0.612 0.367 0.136 0.486 3.41 0.063 0.106 0.021 Glycochenodeoxycholic Acid 0.009 0.047 0.085 0.023 0.006 0 0.031 0 0.001 0.012 0.008 0.456 0.012 0.025 0.002 0.032 0 0.006 0.015 0.007 0.109 0.008 0.926 0.055 0.001 0.058 0 0.012 0 0.015 0.016 0.132 0.013 0.001 0.001 0.046 0 0.006 0.011 0.017 0.009 0.015 0.038 0 0 0 0 0.014 0.009 0.027 0.014 0 0.005 0 0.007 2.5 0.023 0.008 0.025 0.011 0.008 0.006 0.004 0.017 0.004 0.23 0.008 0 0 0 0.001 0 0.008 0.011 0.001 0.389 0.014 0.007 0.012 0.02 0.008 0.035 0.156 0.484 0.075 0 0.014 0 0 0.008 0 0.054 0.011 0 0 0.016 0.008 0.053 0.015 0 0.012 0.008 0.018 0.007 0.041 0 0 0 0.019 0.109 0.007 0.015 0.006 0.037 0.008 0.021 0.016 0 0.077 0.021 0 0.011 0.007 0 0.023 0.008 0.042 0.007 0 0.01 0.162 0.006 0 0 Glycodeoxycholic Acid 0.006 0 0.053 0.009 0.005 0.004 0.008 0.003 0 0.004 0.003 0.38 0.014 0.004 0.007 0.019 0.002 0.022 0.009 0.002 0 0.007 0 0 0.002 0.051 0.005 0.012 0.006 0.001 0.003 0.073 0.002 0.001 0 0.014 0.009 0.009 0.015 0.006 0.002 0.01 0 0 0.003 0.014 0.004 0.009 0.005 0.003 0.013 0.004 0 0.002 0.003 0.154 0.011 0.008 0.042 0.004 0.026 0.002 0.002 0.014 0.003 0.075 0.001 0.013 0.004 0.002 0 0.011 0 0.015 0.002 0.271 0.028 0.005 0.004 0.028 0 0.074 0.114 1.44 0.052 0.005 0.005 0.006 0.007 0.007 0.021 0.006 0.006 0.026 0.017 0.027 0.001 0.047 0 0.018 0.011 0.001 0.055 0.01 0.005 0.011 0.032 0.019 0 0.127 0 0.01 0.01 0.003 0 0.016 0.029 0.006 0.145 0.028 0.035 0 0.008 0.017 0.003 0 0.043 0.007 0.044 0 0.082 0.003 0.002 0.004 Glycolithocholic Acid 0.002 0 0.001 0.002 0.001 0.002 0.001 0.002 0 0.001 0 0.008 0.001 0.001 0 0.001 0.003 0.002 0.003 0.001 0 0.001 0.001 0 0.001 0.004 0.003 0.001 0.001 0.002 0.002 0.002 0.001 0 0 0.002 0.004 0.001 0.003 0.002 0.002 0 0 0.001 0.001 0.001 0.005 0.001 0.002 0.004 0.003 0.001 0 0.004 0.001 0.006 0.003 0 0.001 0 0.002 0 0.003 0.001 0.001 0.001 0.003 0.004 0 0.002 0 0.004 0 0 0.001 0.006 0 0.001 0.003 0.001 0 0.003 0.002 0.018 0.002 0.002 0.001 0 0.01 0.001 0.003 0 0.001 0.003 0.003 0.003 0.001 0.001 0 0.004 0 0.003 0 0.002 0 0.004 0.005 0.003 0 0.002 0.002 0 0.012 0 0 0.002 0.001 0.002 0.006 0.005 0.004 0 0 0.003 0 0 0.002 0.007 0.004 0.001 0.003 0 0 0 Glycoursodeoxycholic Acid 0.002 0.005 0.02 0.007 0.011 0.005 0.015 0.001 0.003 0.002 0.002 3.62 0.035 0.004 0.002 0.092 0.001 0.008 0.002 0.002 0.024 0.012 0.052 0.022 0.001 0.095 0.004 0.037 0.009 0.002 0.03 0.718 0.003 0.008 0.002 0.056 0.002 0.01 0.026 0.018 0.002 0.003 0.006 0.002 0.003 0.009 0.005 0.022 0.002 0.008 0.004 0.003 0.011 0 0.002 0.391 0.003 0.013 0.004 0.017 0.034 0.002 0.002 0.052 0.004 2.11 0.001 0.008 0.001 0.002 0.003 0.01 0.004 0.008 0.001 0.409 0.029 0.017 0.002 0.016 0.013 0.007 0.272 0.718 0.125 0.006 0.046 0.005 0.018 0.019 0.025 0.19 0.021 0.019 0.014 0.024 0.019 0.109 0.007 0.013 0.036 0.011 0.03 0.021 0.159 0.022 0.015 0.02 0.01 0.188 0.011 0.023 0.025 0.03 0.017 0.024 0.034 0.011 0.09 0.044 0.01 0.014 0.035 0.009 0.029 0.006 0.085 0.01 0.04 0.007 0.382 0.007 0.026 0.007 Hyodeoxycholic Acid 0.013 0.013 0.024 0.028 0.02 0.102 0.148 0.299 0.067 0.016 0.013 0.056 0.051 0.014 0.15 0.022 0.609 0.37 0.013 0.013 0.016 0.016 0.022 0.017 0.036 0.023 0.399 0.027 0.086 0.015 0.016 0.067 0.015 0.059 0.027 0.022 0.087 0.017 0.02 0.018 0.013 0.025 0.015 0.017 0.202 1.4 0.567 0.021 0.017 0.095 0.095 0.27 0.024 0.265 0.013 0.164 0.013 0.041 0.02 0.017 0.026 0.101 0.013 0.024 0.076 0.039 0.016 0.484 0.114 0.633 0.06 0.707 0.134 0.113 0.028 0.074 0.034 0.028 0.015 0.07 4.25 0.018 0.123 1.48 0.079 4.24 0.056 1.95 7.32 4.72 22.9 0.093 3.69 9.97 15.5 14.3 4.52 0.061 0.007 13.4 1.56 5.69 0.018 17.6 0.07 8.85 20.5 13.2 0.005 0.129 8.79 0.055 14.3 0.124 0.053 1.83 11 0.266 0.151 15.7 4.35 8.7 18.1 2.89 0.093 0.004 0.094 0.583 21.8 0.325 0.114 0.05 6.73 0.461 Lithocholic Acid 0.003 0 0 0.009 0.005 2.86 0 1.77 0.674 0 0 0 0.019 0 0.337 0 2.35 5.36 0 0 0 0 0 0 0.186 0.007 2.15 0 0.21 0 0 0 0.005 0.04 0.115 0 1.37 0 0 0 0 0 0 0.069 0.439 3.83 2.83 0 0.011 0 0.513 0.853 0.92 5.74 0 0 0.008 0.01 0 0 0 0 0 0 0.307 0 0 2.66 0.052 0.771 0.323 5.3 0 1.12 0.092 0 0.049 0 0.024 0.847 4.2 0 0 0.046 0 3.54 0 1.41 2.58 3.05 7.4 0.097 3.03 5.24 4.61 7.89 3.02 0 0 6.07 2.78 3.01 0 7.1 0 6.79 6.52 6.48 0 0.001 4.12 0 4.66 0 0.117 3.52 5.15 0.052 0.211 5.56 3.43 5 3.86 4.09 0.024 0 0 0.135 7.97 0 0 0 4.57 0.355 α-Muricholic Acid 0.073 0.027 0.233 0.614 0.046 2.03 8.05 16.2 2.94 0.302 0.014 0.25 0.672 0.018 11.8 0.008 28.1 82.8 0.17 0.007 0.029 0.006 0.154 2.37 11.1 0.042 0 0.238 14.5 0.045 0.038 0.731 0.152 0 11.4 0.488 2 0.041 0.06 0.008 0.007 0.056 0.029 1.61 0 0.286 0.812 0.008 0.028 6.05 6.77 0.215 2.86 10 0.009 6.81 0.024 0.26 0.116 0.008 0.049 34.1 0.009 0.103 16.4 0.41 0.502 0.499 19.1 41.1 21.2 1.53 9.71 43.8 7.19 0.087 0.151 0.027 0.018 21.3 0.96 0.049 0.423 0.904 0.113 0.505 0.017 0.707 1.74 NA 6.43 0.299 0.153 5.55 4.99 3.65 0.173 0.337 0.011 1.58 0.331 0.146 0.011 1.8 0.015 0.264 5.36 5.56 0.022 0.061 0.895 0.053 0.6 0.892 0.005 1.94 0.809 4.79 0.173 0.98 NA 0.438 0.018 2.1 0.25 0.011 0.038 NA NA 3.03 0.356 3.68 0.593 3.23 β-Muricholic Acid 0.042 0.105 1.47 0.694 0.229 25.8 31.4 10.8 1.75 0.223 0.122 1.12 1.39 0.137 35 0.093 18.2 55.8 0.11 0.082 0.776 0.068 0.136 2.36 7.94 0.084 8.04 0.154 10.1 0.294 0.058 1.82 0 0.743 7.72 0.344 27 0.02 0.163 0.049 0.03 0.305 0.079 1.11 2.41 22.8 17.5 0.133 0.625 4.49 22.8 6.82 1.72 6.49 0.065 1.62 0.265 0.811 0.464 0.049 0.092 23.2 0.056 0.332 12.2 1.57 0.311 14 29.6 28.8 13.1 16 30 29.6 4.96 0.487 0.282 0.217 0.223 15.9 11.6 0.33 0.743 2.51 0.12 11.8 0.18 7.45 13.9 36.7 34.8 0.639 4.33 47.1 33.8 30.8 10.8 0.016 0.391 15.1 2.53 10.2 0.095 21.8 0.059 11.7 49.5 37.3 0.249 0.264 32.4 0.223 24.2 0.548 0.031 1.88 11.9 18.6 0.289 21.1 52.4 13.6 8.98 21.2 0.283 0.123 0.126 41 60.7 18.4 0.273 6.09 19.4 19.5 ω-Muricholic Acid 0.019 0.025 0.063 0.157 0.044 16.1 11.4 10.9 2.91 0.017 0.006 0.722 0.332 0.005 10.3 0.025 15.8 4.47 0.065 0.005 0.073 0.011 0.012 1.8 1.11 0.015 17.3 0.018 3.12 0.009 0.017 0.436 0.034 2.21 0.826 0.079 28.2 0.015 0.049 0.032 0.002 0.257 0.002 2.73 9.02 9.92 15.4 0.082 0.028 3.43 1.63 4.06 2.27 35.8 0.002 0.631 0.017 0.074 0.072 0.019 0.04 4.01 0.008 0.048 0.864 0.62 0.019 14.1 10.6 9.81 1.09 13 2.92 17.8 0.831 0.162 0.091 0.068 0.005 1.94 16.6 0.461 0.106 1.5 0.042 7.85 0.048 5.17 5.34 18.6 20 0.097 15.4 44.8 38.2 19.1 8.36 0.085 0.011 19.4 10.1 7.52 0.063 27.2 0.022 24.3 35.8 29.6 0.003 0.068 29.5 0.059 14.5 0.049 0.016 23 21.6 1.38 0.208 31.9 73.2 10.1 12.6 55.6 0.029 0.001 0.11 2.97 38.6 0.926 0.051 0.497 14.8 0.748 Taurocholic Acid 4.33 7.2 4.7 2.32 0 0.273 14.8 0.05 0.009 2.4 2.86 2.57 0.5 4.16 9.75 6.89 0.043 6.84 5.71 2.48 16.1 3.56 2.46 16.6 2.87 5.78 0.088 1.71 0.387 3.64 0.763 1.34 2.58 0 0.314 8.08 0.28 2.74 1.29 7.34 1.64 2.11 6.43 0.057 0.013 0.422 0.075 7.86 5.3 0.185 14 0.108 8.4 0.028 2.16 2.93 11.6 1.17 1.86 5.38 2.28 0.353 0.666 4.61 7.7 3.6 3.23 0.156 4.99 0.1 0.102 0.648 10.8 0.839 0.091 0.834 0.46 5.94 5.31 13 0.159 3.14 2.91 0.77 0.302 0.722 1.91 0.058 1.3 0.853 1.13 6.99 0.209 0.368 0.352 1.03 0.224 0.36 2.06 0.249 0.078 0.077 1.66 5.98 2.75 0.268 2.36 0.215 4.96 0.848 0.552 0.63 0.727 0.739 0.765 0.058 0.059 1.27 0.162 0.209 0.111 0.14 0.213 0.876 0.419 0.43 1.05 27 1.31 13.8 4.36 7.03 0.404 1.77 Taurochenodeoxycholic Acid 0.243 1.45 0.511 0.37 0.161 0.007 0.386 0.006 0.001 0.444 0.333 0.373 0.11 0.434 0.062 0.468 0.005 0.006 0.525 0.294 2.45 0.232 0.409 3.16 0.025 0.597 0.001 0.199 0.012 0.562 0.173 0.19 0.417 0 0.012 0.643 0.01 0.149 0.167 0.737 0.289 0.528 1.1 0.006 0.002 0.018 0.002 0.644 0.279 0.008 0.128 0.006 0.101 0.007 0.202 1.34 1.37 0.268 0.128 0.39 0.144 0.069 0.088 0.343 0.182 0.758 0.195 0.006 0.018 0.006 0.015 0.033 0.098 0.021 0.009 0.244 0.074 0.308 0.459 1.3 0.039 0.165 0.323 0.244 0.079 0.036 0.17 0.013 0.043 0.09 0.193 0.989 0.059 0.016 0.053 0.126 0.047 0.049 0.211 0.025 0.023 0.026 0.125 0.068 0.505 0.068 0.231 0.031 0.431 0.188 0.034 0.085 0.063 0.216 0.113 0.027 0.013 0.065 0.051 0.026 0.024 0.044 0.024 0.051 0.128 0.045 0.113 0.142 0.113 0.042 0.205 0.143 0.081 0.029 Taurodeoxycholic Acid 0.162 1.27 0.344 0.068 0.072 0.011 0 0.007 0.002 0.041 0.075 0.111 0.044 0.058 0.059 0.303 0.006 0.074 0.224 0.069 0 0.176 0 0 0.037 0.297 0.009 0.127 0.005 0.007 0.021 0.042 0.038 0.001 0.003 0.112 0.014 0.286 0.112 0.159 0.009 0.252 0 0.002 0.002 0.022 0.007 0.379 0.106 0.002 0.174 0.008 0 0.003 0.072 0.029 0.513 0.149 0.126 0.159 0.194 0 0.005 0.211 0.017 0.15 0 0.008 0 0.007 0.005 0.031 0 0.038 0.004 0.074 0.069 0.242 0.064 1.05 0.01 0.278 0.116 0.144 0.032 0.053 0.082 0.008 0.056 0.059 0.19 0.172 0.039 0.404 0.049 0.089 0.015 0.025 0.003 0.033 0.032 0.008 0.289 0.034 0.096 0.028 1.06 0.144 0.443 0.088 0.14 0.099 0.065 0.035 0.11 0.007 0.006 0.03 0.051 0.035 0.209 0.016 0.004 0.124 0.034 0.003 0.099 0.004 0.38 0.003 0.092 0.003 0.022 0.029 Taurolithocholic Acid 0.001 0 0.005 0.003 0.002 0.002 0 0.001 0 0.001 0 0.009 0.002 0.001 0.001 0.002 0.002 0.008 0.001 0 0.001 0.002 0.002 0.003 0 0.004 0.001 0.003 0.001 0 0.002 0.004 0.001 0 0 0.003 0.001 0.002 0.002 0.002 0 0.005 0.001 0 0 0.002 0.001 0.002 0.002 0.004 0.002 0 0 0.001 0.001 0.003 0.009 0.002 0.002 0.003 0.004 0 0.001 0.002 0.001 0.01 0.001 0.002 0 0 0 0.003 0 0 0 0.006 0.002 0.002 0.002 0.023 0.003 0.003 0.004 0.011 0.003 0.004 0.002 0.003 0.008 0.007 0.015 0.005 0.004 0.015 0 0.01 0.003 0.003 0.002 0.006 0.004 0.004 0.002 0.006 0.004 0.007 0.025 0.01 0.003 0.003 0.007 0.002 0.009 0.005 0.003 0.003 0.006 0.003 0.005 0 0.011 0.004 0 0.01 0.005 0.002 0.004 0.005 0.015 0.003 0.006 0.021 0.005 0.002 α-β-Tauromuricholic Acid 1.54 NA 1.16 0.755 0.437 0.091 3.52 0.194 0.013 2.12 1.78 2.28 0.316 2.08 7.59 1.72 0.073 2.51 2.67 1.44 NA 0.78 0.631 4.69 0.439 1.17 0.043 0.574 0.164 2.06 0.283 0.969 0.886 0.033 0.591 2.02 0.119 0.544 0.35 2.6 0.632 1.03 3.33 0.109 0.021 0.313 0.029 2.84 1.76 0.035 NA 0.089 2.74 0.043 0.793 7.26 NA 0.641 0.318 1.26 0.315 0.219 0.333 0.949 2.46 3.03 1.35 0.057 5.24 1.42 0.115 0.258 NA 0.175 0.055 0.351 0.159 1.17 2.87 NA 0.182 0.812 1.48 1.2 0.16 0.532 0.947 0.038 0.774 1.37 1.76 4.73 0.274 3.74 0.471 0.525 0.271 0.138 1.05 0.151 0.098 0.112 0.604 0.423 2.39 0.498 2.94 0.275 3.92 0.424 1.31 0.295 0.452 0.676 0.326 0.115 0.042 1.41 0.131 0.129 0.263 0.212 0.151 0.708 0.471 0.194 0.302 5.55 1.66 1.69 0.879 4.25 0.216 NA Tauroursodeoxycholic Acid 0.103 0.193 0.073 0.124 0.285 0.013 0.206 0.033 0.007 0.052 0.064 3.03 0.346 0.043 0.037 1.17 0.032 0.022 0.129 0.043 0.613 0.433 0.022 1.95 0.025 0.885 0.018 0.77 0.011 0.115 0.147 1.07 0.035 0.017 0.012 0.82 0.009 0.4 0.405 1.02 0.022 0.201 0.078 0.004 0.015 0.14 0.022 1.11 0.056 0.004 0.074 0.02 0.073 0.009 0.028 0.479 0.533 0.505 0.025 0.68 0.603 0.035 0.013 1.01 0.06 4.83 0.043 0.019 0.017 0.021 0.014 0.201 0.068 0.022 0.006 0.219 0.183 0.917 0.086 1.16 0.078 0.065 0.862 0.531 0.118 0.14 0.63 0.022 0.272 0.289 0.707 1.75 0.187 0.347 0.144 0.264 0.11 0.107 0.031 0.1 0.066 0.061 0.345 0.226 1.05 0.208 1.09 0.122 0.068 0.329 0.237 0.19 0.158 0.21 0.294 0.071 0.026 0.027 0.074 0.099 0.025 0.083 0.095 0 0.145 0.012 0.222 0.07 0.946 0.032 0.792 0.06 0.089 0.046 Ursodeoxycholic Acid 0 0 0.169 2.15 1.43 23.8 0.479 63.2 10.1 0 0 24.6 13.7 0 0.035 1.85 87 5.37 0 0 0.095 0.865 0 0 0 2.61 119 3.56 0 0 0.678 22 0.096 14.8 0 2.34 10.9 0.758 3.37 1.03 0 1.1 0 1.23 48.5 162 128 2.1 0 0 0.183 83.4 0 53.5 0 1.12 0.142 7.02 0.055 1.24 3.76 0 0 4.95 0 25.8 0 101 0.121 4.13 0 176 0 7.61 0 8.99 5.18 3.85 0 6.15 179 0.65 26.4 64.1 7.79 261 3.57 110 241 193 435 7.55 189 374 420 448 198 12 0 405 101 290 1.67 515 3.76 353 548 522 0 8.7 339 3.29 361 9.3 2.46 97.6 477 2.2 11.2 499 254 291 468 146 4.21 0 6.27 1.75 697 0.479 17.5 0.843 223 0.58 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Name KEGG Cholic Acid CA C00695 Chenodeoxycholic Acid CDCA C02528 Deoxycholic Acid DCA C04483 Glycocholic Acid GCA C01921 Glycochenodeoxycholic Acid GCDCA C05466 Glycodeoxycholic Acid GDCA C05464 Glycolithocholic Acid GLCA C15557 Glycoursodeoxycholic Acid GUDCA Hyodeoxycholic Acid HDCA C15517 Lithocholic Acid LCA C03990 α-Muricholic Acid MCA(a) C17647 β-Muricholic Acid MCA(b) C17726 ω-Muricholic Acid MCA(o) C17727 Taurocholic Acid TCA C02258 Taurochenodeoxycholic Acid TCDCA C05465 Taurodeoxycholic Acid TDCA C05463 Taurolithocholic Acid TLCA C02592 α-β-Tauromuricholic Acid TMCA(a+b) Tauroursodeoxycholic Acid TUDCA C16868 Ursodeoxycholic Acid UDCA C07880 METABOLITES_END #END