#METABOLOMICS WORKBENCH Andressa_Stuart_20210929_131232 DATATRACK_ID:2873 STUDY_ID:ST001927 ANALYSIS_ID:AN003133 PROJECT_ID:PR001217 VERSION 1 CREATED_ON October 18, 2021, 10:26 am #PROJECT PR:PROJECT_TITLE Fungal consortium of two Beauveria bassiana strains increases their virulence, PR:PROJECT_TITLE growth, and resistance to stress: a metabolomic approach. PR:PROJECT_TYPE Untargeted Metabolomics PR:PROJECT_SUMMARY Entomopathogenic fungi have been successfully used to control agricultural PR:PROJECT_SUMMARY pests. They infect insects by coming into direct contact with their cuticle or PR:PROJECT_SUMMARY when feeding on contaminated leaves or fruits. After contact with the insect, PR:PROJECT_SUMMARY the entomopathogenic fungus penetrates its body cavity, where it grows and PR:PROJECT_SUMMARY colonizes it from within, causing its death The use of two or more PR:PROJECT_SUMMARY microorganisms in a microbial consortium has been increasingly applied in the PR:PROJECT_SUMMARY biological control of diseases and pests. Beauveria bassiana is one of the most PR:PROJECT_SUMMARY widely studied fungal species in biological control, yet little is known about PR:PROJECT_SUMMARY its role in fungal consortiums. In a previous study, our group found that a PR:PROJECT_SUMMARY consortium formed by two strains of B. bassiana had significantly greater PR:PROJECT_SUMMARY biocontrol potential against the polyphagous caterpillars Duponchelia fovealis PR:PROJECT_SUMMARY (Lepidoptera: Crambidae) than either strain on its own. Despite recent PR:PROJECT_SUMMARY developments and growing efforts to better understand fungal metabolism and PR:PROJECT_SUMMARY metabolites, much remains unknown. Metabolomics therefore represents an PR:PROJECT_SUMMARY important field for evaluating the metabolites produced or modified by an PR:PROJECT_SUMMARY organism or its relationship with the environment. In the present study, we aim PR:PROJECT_SUMMARY to use untargeted metabolomics with gas and liquid chromatography coupled to PR:PROJECT_SUMMARY mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations PR:PROJECT_SUMMARY caused by the co-cultivation of these strains and to correlate the metabolites PR:PROJECT_SUMMARY produced by this consortium with the increased mortality in D. fovealis observed PR:PROJECT_SUMMARY previously. PR:INSTITUTE Universidade Federal do Paraná PR:DEPARTMENT Patologia Básica PR:LABORATORY Laboratório de Microbiologia e Biologia Molecular PR:LAST_NAME Stuart PR:FIRST_NAME Andressa PR:ADDRESS Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, PR:ADDRESS Curitiba, Paraná, Brasil PR:EMAIL andressa.katiski@gmail.com PR:PHONE 5541991922779 PR:PROJECT_COMMENTS Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were PR:PROJECT_COMMENTS cultivated in Agar Sabouraud culture medium, both separately and co-cultivated PR:PROJECT_COMMENTS to form a fungal consortium. The metabolomic analysis were performed at the PR:PROJECT_COMMENTS Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior PR:PROJECT_COMMENTS de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). PR:PROJECT_COMMENTS Three reads of every biological replicate (five per treatment) were performed, PR:PROJECT_COMMENTS generating fifteen readings for each treatment. Pools of metabolites from each PR:PROJECT_COMMENTS group were created as a quality control. PR:CONTRIBUTORS Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria PR:CONTRIBUTORS Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel #STUDY ST:STUDY_TITLE Fungal consortium of two Beauveria bassiana strains increases their virulence, ST:STUDY_TITLE growth, and resistance to stress: a metabolomic approach. ST:STUDY_TYPE Untargeted Metabolomics ST:STUDY_SUMMARY Entomopathogenic fungi have been successfully used to control agricultural ST:STUDY_SUMMARY pests. They infect insects by coming into direct contact with their cuticle or ST:STUDY_SUMMARY when feeding on contaminated leaves or fruits. After contact with the insect, ST:STUDY_SUMMARY the entomopathogenic fungus penetrates its body cavity, where it grows and ST:STUDY_SUMMARY colonizes it from within, causing its death The use of two or more ST:STUDY_SUMMARY microorganisms in a microbial consortium has been increasingly applied in the ST:STUDY_SUMMARY biological control of diseases and pests. Beauveria bassiana is one of the most ST:STUDY_SUMMARY widely studied fungal species in biological control, yet little is known about ST:STUDY_SUMMARY its role in fungal consortiums. In a previous study, our group found that a ST:STUDY_SUMMARY consortium formed by two strains of B. bassiana had significantly greater ST:STUDY_SUMMARY biocontrol potential against the polyphagous caterpillars Duponchelia fovealis ST:STUDY_SUMMARY (Lepidoptera: Crambidae) than either strain on its own. Despite recent ST:STUDY_SUMMARY developments and growing efforts to better understand fungal metabolism and ST:STUDY_SUMMARY metabolites, much remains unknown. Metabolomics therefore represents an ST:STUDY_SUMMARY important field for evaluating the metabolites produced or modified by an ST:STUDY_SUMMARY organism or its relationship with the environment. In the present study, we aim ST:STUDY_SUMMARY to use untargeted metabolomics with gas and liquid chromatography coupled to ST:STUDY_SUMMARY mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations ST:STUDY_SUMMARY caused by the co-cultivation of these strains and to correlate the metabolites ST:STUDY_SUMMARY produced by this consortium with the increased mortality in D. fovealis observed ST:STUDY_SUMMARY previosly. ST:INSTITUTE Universidade Federal do Paraná ST:DEPARTMENT Patologia Básica ST:LABORATORY Laboratório de Microbiologia e Biologia Molecular ST:LAST_NAME Stuart ST:FIRST_NAME Andressa ST:ADDRESS Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, ST:ADDRESS Curitiba, Paraná, Brasil ST:EMAIL andressa.katiski@gmail.com ST:PHONE 5541991922779 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 15 ST:STUDY_COMMENTS Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were ST:STUDY_COMMENTS cultivated in Agar Sabouraud culture medium, both separately and co-cultivated ST:STUDY_COMMENTS to form a fungal consortium. The metabolomic analysis were performed at the ST:STUDY_COMMENTS Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior ST:STUDY_COMMENTS de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). ST:STUDY_COMMENTS Three reads of every biological replicate (five per treatment) were performed, ST:STUDY_COMMENTS generating fifteen readings for each treatment. Pools of metabolites from each ST:STUDY_COMMENTS group were created as a quality control. #SUBJECT SU:SUBJECT_TYPE Fungi SU:SUBJECT_SPECIES Beauveria bassiana SU:GENOTYPE_STRAIN GenBank: KU751847; KU751848 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Bov2_1 Fungal species:Beauveria bassiana Strain Bov2 RAW_FILE_NAME=Bov2_1; RAW_FILE_NAME=Bov2_1_R001; Bov2_1_R002; Bov2_1_R003; RAW_FILE_NAME=Bov2_1_R001_NEG; Bov2_1_R002_NEG; Bov2_1_R003_NEG; Group=Bov2 SUBJECT_SAMPLE_FACTORS - Bov2_2 Fungal species:Beauveria bassiana Strain Bov2 RAW_FILE_NAME=Bov2_2; RAW_FILE_NAME=Bov2_2_R001; Bov2_2_R002; Bov2_2_R003; RAW_FILE_NAME=Bov2_2_R001_NEG; Bov2_2_R002_NEG; Bov2_2_R003_NEG; Group=Bov2 SUBJECT_SAMPLE_FACTORS - Bov2_3 Fungal species:Beauveria bassiana Strain Bov2 RAW_FILE_NAME=Bov2_3; RAW_FILE_NAME=Bov2_3_R001; Bov2_3_R002; Bov2_3_R003; RAW_FILE_NAME=Bov2_3_R001_NEG; Bov2_3_R003_NEG; Bov2_3_R003_NEG; Group=Bov2 SUBJECT_SAMPLE_FACTORS - Bov2_4 Fungal species:Beauveria bassiana Strain Bov2 RAW_FILE_NAME=Bov2_4; RAW_FILE_NAME=Bov2_4_R001; Bov2_4_R002; Bov2_4_R003; RAW_FILE_NAME=Bov2_4_R001_NEG; Bov2_4_R002_NEG; Bov2_4_R003_NEG; Group=Bov2 SUBJECT_SAMPLE_FACTORS - Bov2_5 Fungal species:Beauveria bassiana Strain Bov2 RAW_FILE_NAME=Bov2_5; RAW_FILE_NAME=Bov2_5_R001; Bov2_5_R002; Bov2_5_R003; RAW_FILE_NAME=Bov2_5_R001_NEG; Bov2_5_R002_NEG; Bov2_5_R003_NEG; Group=Bov2 SUBJECT_SAMPLE_FACTORS - Bov3_1 Fungal species:Beauveria bassiana Strain Bov3 RAW_FILE_NAME=Bov3_1; RAW_FILE_NAME=Bov3_1_R001; Bov3_1_R002; Bov3_1_R003; RAW_FILE_NAME=Bov3_1_R001_NEG; Bov3_1_R002_NEG; Bov3_1_R003_NEG; Group=Bov3 SUBJECT_SAMPLE_FACTORS - Bov3_2 Fungal species:Beauveria bassiana Strain Bov3 RAW_FILE_NAME=Bov3_2; RAW_FILE_NAME=Bov3_2_R001; Bov3_2_R002; Bov3_2_R003; RAW_FILE_NAME=Bov3_2_R001_NEG; Bov3_2_R002_NEG; Bov3_2_R003_NEG; Group=Bov3 SUBJECT_SAMPLE_FACTORS - Bov3_3 Fungal species:Beauveria bassiana Strain Bov3 RAW_FILE_NAME=Bov3_3; RAW_FILE_NAME=Bov3_3_R001; Bov3_3_R002; Bov3_3_R003; RAW_FILE_NAME=Bov3_3_R001_NEG; Bov3_3_R002_NEG; Bov3_3_R003_NEG; Group=Bov3 SUBJECT_SAMPLE_FACTORS - Bov3_4 Fungal species:Beauveria bassiana Strain Bov3 RAW_FILE_NAME=Bov3_4; RAW_FILE_NAME=Bov3_4_R001; Bov3_4_R002; Bov3_4_R003; RAW_FILE_NAME=Bov3_4_R001_NEG; Bov3_4_R002_NEG; Bov3_4_R003_NEG; Group=Bov3 SUBJECT_SAMPLE_FACTORS - Bov3_5 Fungal species:Beauveria bassiana Strain Bov3 RAW_FILE_NAME=Bov3_5; RAW_FILE_NAME=Bov3_5_R001; Bov3_5_R002; Bov3_5_R003; RAW_FILE_NAME=Bov3_5_R001_NEG; Bov3_5_R002_NEG; Bov3_5_R003_NEG; Group=Bov3 SUBJECT_SAMPLE_FACTORS - Consortium_1 Fungal species:Consortium formed by Bov2 and Bov3 strains RAW_FILE_NAME=Cons_1; RAW_FILE_NAME=Cons_1_R001; Cons_1_R002; Cons_1_R003; RAW_FILE_NAME=Cons_1_R001_NEG; Cons_1_R002_NEG; Cons_1_R003_NEG; Group=Cons SUBJECT_SAMPLE_FACTORS - Consortium_2 Fungal species:Consortium formed by Bov2 and Bov3 strains RAW_FILE_NAME=Cons_2; RAW_FILE_NAME=Cons_2_R001; Cons_2_R002; Cons_2_R003; RAW_FILE_NAME=Cons_2_R001_NEG; Cons_2_R002_NEG; Cons_2_R003_NEG; Group=Cons SUBJECT_SAMPLE_FACTORS - Consortium_3 Fungal species:Consortium formed by Bov2 and Bov3 strains RAW_FILE_NAME=Cons_3; RAW_FILE_NAME=Cons_3_R001; Cons_3_R002; Cons_3_R003; RAW_FILE_NAME=Cons_3_R001_NEG; Cons_3_R002_NEG; Cons_3_R003_NEG; Group=Cons SUBJECT_SAMPLE_FACTORS - Consortium_4 Fungal species:Consortium formed by Bov2 and Bov3 strains RAW_FILE_NAME=Cons_4; RAW_FILE_NAME=Cons_4_R001; Cons_4_R002; Cons_4_R003; RAW_FILE_NAME=Cons_4_R001_NEG; Cons_4_R002_NEG; Cons_4_R003_NEG; Group=Cons SUBJECT_SAMPLE_FACTORS - Consortium_5 Fungal species:Consortium formed by Bov2 and Bov3 strains RAW_FILE_NAME=Cons_5; RAW_FILE_NAME=Cons_5_R001; Cons_5_R002; Cons_5_R003; RAW_FILE_NAME=Cons_5_R001_NEG; Cons_5_R002_NEG; Cons_5_R003_NEG; Group=Cons #COLLECTION CO:COLLECTION_SUMMARY Two genetically distinct strains of Beauveria bassiana (Bov 3 and Bov 2) were CO:COLLECTION_SUMMARY cultivated both separately and co-cultivated to form a fungal consortium. After CO:COLLECTION_SUMMARY the colonies had grown, the mycelium of each treatment (Bov 2, Bov 3, and the CO:COLLECTION_SUMMARY fungal consortium) was scraped from the culture medium with a spatula and then CO:COLLECTION_SUMMARY macerated separately in liquid nitrogen (N2) CO:COLLECTION_PROTOCOL_FILENAME MetaboliteExtraction CO:SAMPLE_TYPE Fungal mycelium CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were TR:TREATMENT_SUMMARY cultivated in Petri dishes containing Agar Sabouraud culture medium, both TR:TREATMENT_SUMMARY separately and co-cultivated to form a fungal consortium. The cultures were TR:TREATMENT_SUMMARY incubated in the dark in a biological oxygen demand (BOD) oven for 14 days at TR:TREATMENT_SUMMARY 28°C. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Extraction was performed in microtubes, from 200 mg of fungal macerate to which SP:SAMPLEPREP_SUMMARY 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. SP:SAMPLEPREP_SUMMARY These extraction microtubes were vigorously vortexed and placed in an ultrasonic SP:SAMPLEPREP_SUMMARY low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged SP:SAMPLEPREP_SUMMARY (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was SP:SAMPLEPREP_SUMMARY filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a SP:SAMPLEPREP_SUMMARY chromatographic vial where the extracts were lyophilized (Thermo Fischer SP:SAMPLEPREP_SUMMARY Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were SP:SAMPLEPREP_SUMMARY resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS SP:SAMPLEPREP_SUMMARY and LC-MS/MS. SP:PROCESSING_STORAGE_CONDITIONS -80℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY (.cdf) CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) CH:FLOW_RATE 1 mL.min-1 CH:COLUMN_TEMPERATURE 70 - 320 ºC CH:METHODS_FILENAME LCMSMS CH:INJECTION_TEMPERATURE 280 ºC CH:SAMPLE_INJECTION 1 uL CH:OVEN_TEMPERATURE 70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then CH:OVEN_TEMPERATURE held at this temperature for 4 min. #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Leco Pegasus 4D GCxGC TOF MS:INSTRUMENT_TYPE GC x GC-TOF MS:MS_TYPE EI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS Data from GC-MS was processed using ChromaTOF 4.32 software. NIST library MS:MS_COMMENTS version 11 was used for the identification of metabolites. MS:ION_SOURCE_TEMPERATURE 250 ºC MS:IONIZATION_ENERGY 70 eV #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak area MS_METABOLITE_DATA_START Samples Consortium_1 Consortium_2 Consortium_3 Consortium_4 Consortium_5 Bov3_1 Bov3_2 Bov3_3 Bov3_4 Bov3_5 Bov2_1 Bov2_2 Bov2_3 Bov2_4 Bov2_5 Factors Fungal species:Consortium formed by Bov2 and Bov3 strains Fungal species:Consortium formed by Bov2 and Bov3 strains Fungal species:Consortium formed by Bov2 and Bov3 strains Fungal species:Consortium formed by Bov2 and Bov3 strains Fungal species:Consortium formed by Bov2 and Bov3 strains Fungal species:Beauveria bassiana Strain Bov3 Fungal species:Beauveria bassiana Strain Bov3 Fungal species:Beauveria bassiana Strain Bov3 Fungal species:Beauveria bassiana Strain Bov3 Fungal species:Beauveria bassiana Strain Bov3 Fungal species:Beauveria bassiana Strain Bov2 Fungal species:Beauveria bassiana Strain Bov2 Fungal species:Beauveria bassiana Strain Bov2 Fungal species:Beauveria bassiana Strain Bov2 Fungal species:Beauveria bassiana Strain Bov2 1-Deoxypentitol NA NA NA NA NA 0.409139361 0.281160094 0.13491179 0.255208093 NA NA NA NA NA NA 1-Monopalmitin 7.966287942 6.2818918 3.566443807 7.102940942 7.231228635 2.551616656 2.382007123 2.401057923 3.766707942 5.377562489 2.5180544 2.951177673 3.570939285 3.921783738 7.597198629 1,2-Hexadecanediol NA 0.288615647 NA 0.085819875 0.040063691 NA NA NA NA NA 0.038957767 0.110599612 0.112906218 0.117030561 NA 1,2,3,4-tetrahydro-6-methoxy-1-salicyl-7-Isoquinolinol NA NA NA NA NA NA NA NA NA NA 0.017795613 NA NA 0.007110552 0.014999844 1,3-Butanediol 0.680746009 1.070623846 21.73680432 5.992704409 2.56261244 0.345311186 0.539299082 0.181811858 3.364469224 0.817212229 0.489424277 0.917808055 0.74507159 0.586623721 0.67671169 1,4-lactone, Allonic acid 0.910390759 NA NA 0.686571107 0.171973517 NA NA NA NA NA NA NA NA NA NA 1,5-Anhydro-D-sorbitol 7.461646012 NA 25.82836121 NA 10.66734044 NA NA NA NA NA NA NA NA NA NA 2'-Deoxyadenosine 1.630165505 2.024904281 0.68427646 1.253465873 3.183576541 0.388625642 0.183690642 0.284554109 0.431170595 0.272990405 0.621718759 0.638569748 0.893947105 0.601726389 NA 2-Deoxy-erythro-pentonic acid NA NA NA NA NA 2.847375592 0.037056766 2.499410417 NA 1.726039541 NA NA NA NA NA 2-deoxy-D-Erythro-Pentopyranose NA NA NA NA NA NA NA NA NA NA NA 0.170174837 3.198758066 NA 4.441412906 2-Hydroxyglutaric acid NA NA NA NA NA 2.3682551 NA 0.02285147 1.854798113 1.131251437 NA NA NA NA NA 2-oxo-Propanoic acid NA NA NA NA NA 0.022423911 0.638281925 0.480715648 NA NA NA NA NA NA NA 2-Phosphoglyceric acid 0.536320177 1.584792806 0.970306821 NA 0.262925917 NA 0.065890075 1.183942508 NA 1.391821077 NA NA NA NA NA 2-pyrrolidinone 0.161607938 0.101202002 NA NA 0.062974318 0.183621673 0.003202938 0.074184372 0.074152302 0.151596554 0.076527077 0.103104644 0.065007982 0.146252401 0.123303058 2,5-Dimethoxymandelic acid 8.473597377 6.212892959 7.426219504 NA 10.87825898 3.566911023 0.574274278 NA 0.022238953 1.13100024 NA NA 5.494522179 5.100469471 7.544246111 3-Deoxyhexitol 41.91474596 21.33064987 43.06216314 50.20780503 39.76483764 17.18644038 19.05998874 30.86780841 24.20691747 31.91575287 41.16073043 22.34643619 13.89739614 14.47110657 19.46786198 4-Aminobutanoic acid 18.12858277 50.32908557 24.66811543 28.8565268 40.74231036 38.12854247 4.785137774 25.58829677 4.7108517 8.21506383 40.43153627 34.64814977 23.57295286 57.48192827 82.90235817 4-Hydroxybutanoic acid 0.077057043 NA 0.014215184 0.180627308 0.095997256 0.14517337 NA 0.021170547 0.09096275 0.209984746 0.041973902 0.065022592 0.169184958 0.081068017 0.148376805 5-Hydroxypipecolic acid NA NA NA NA NA 0.194414855 0.393959132 0.505027858 0.786342578 1.528793087 NA NA NA NA NA 5-Methyluridine 5.641809597 0.486316774 0.310367729 0.735464958 1.296357889 0.47736102 NA 0.247430402 0.330115739 NA 0.603942034 1.304948371 0.63078825 0.402402303 0.299622331 6-Fluoro-alpha-methyltryptamine NA NA NA NA NA 48.42215019 0.060745722 17.23515772 23.46830526 55.4888943 NA 9.180771456 77.45690529 11.95956277 57.85807869 Acetic acid 0.650374682 0.365222892 0.314978778 0.157187978 0.382455932 0.258064923 0.264599852 0.283698861 0.154584287 0.122632313 0.152413718 0.393780999 0.185285802 0.077665323 0.240230025 alpha-D-(+)-Talopyranose 0.50910139 NA NA 11.02782588 0.079122228 NA 2.002394912 2.476865657 7.080595342 2.115459577 NA 8.129167502 NA 0.729175709 4.569910716 Adenosine 12.82356937 4.274919506 2.052551938 4.592890831 5.29817561 3.207602729 1.660976025 1.584590447 2.341588088 0.724368869 5.342553081 15.47006644 7.439546801 3.4637979 3.04476765 Adenosine-5'-monophosphate 0.262394064 0.247455635 0.120167584 0.198538448 0.29719699 NA NA NA NA NA 0.293289238 0.152325274 0.083837666 NA NA Adrenaline NA NA NA NA NA NA 0.042490157 0.038935427 25.58648693 NA NA NA NA NA NA Ala-beta-Ala NA 0.247221276 NA 0.813899285 0.528041036 NA NA NA NA NA 0.346702814 NA 0.022792445 NA 0.154732971 Alanine 53.71740396 39.98388496 31.7857085 43.37882706 53.68973875 125.3741915 86.77942905 48.71166878 37.77382618 42.6374017 13.93729431 50.04913995 31.66209718 66.40998115 33.39439028 Alanylglycine 0.017389651 0.219667123 35.66175427 NA NA NA NA NA NA NA 0.028957321 0.030457893 0.793629344 0.139517632 NA alpha-D-Mannopyranose 19.09638846 14.45392416 0 25.34304638 35.01676083 0 0 0 0 0 10.6562069 12.18584365 23.20458265 22.1039547 22.54529376 Aminomalonic acid NA 0.127205249 0.055799329 0.886982836 NA 0.383713274 0.200014006 0.215052365 0.224085223 NA 1.22977226 NA 1.038697081 1.297947175 1.330577981 Ammelide 17.71054512 NA 11.24897015 3.599175941 1.653822956 NA 1.400382795 1.70331218 NA 3.020647229 NA NA NA NA NA Asparagine 12.82876648 9.223791161 23.00128116 16.08711751 10.0843478 15.07551657 9.911325561 42.90015655 5.517011373 13.9363052 9.554657413 2.012777109 6.29285125 9.757603785 14.32879311 Aucubin 0.384711624 8.237519464 22.99486318 18.402709 9.703232895 8.952156599 15.91095962 19.04189978 2.783787061 3.961572106 14.55265234 15.81983186 11.53041705 4.793864659 5.505414709 b-Aminoisobutyric acid NA 0.12224078 10.0069721 1.370159355 NA NA NA NA NA NA NA NA NA NA NA Butanedioic acid NA NA NA NA NA 19.32804614 16.70353583 12.25298758 NA 9.546804121 5.720590128 7.204484215 5.00066952 NA NA cis-4-Decene-1,10-dioic acid NA NA NA NA NA NA NA NA NA NA NA NA 2.360362809 0.710152244 1.158915804 Citric acid 12.13468276 47.71232887 45.61472076 38.37161026 2.785967601 17.67753987 16.97675694 16.51606477 23.02835822 48.2443176 28.59193343 12.06675301 20.25170827 13.78394748 16.33904167 Cystathionine 6.643058031 8.058065942 7.909127541 8.200961255 9.515088116 5.575503932 4.679830264 7.995227769 2.822105813 6.924526407 6.669202932 1.098653944 1.685970082 2.962897281 6.821146313 Cysteine 1.081357117 0.791115065 0.408419767 0.718017853 0.400331077 2.269142575 1.099431643 1.285731358 1.822910338 1.131251437 1.14031342 0.130588141 1.113823268 0.95245079 0.68975153 D-(-)-Fructofuranose NA NA NA NA NA 2.853134806 0.590158103 0.279863632 NA 2.762696392 0.821518351 5.487684324 NA NA 0.218820353 D-(+)-Talofuranose 2.036608352 1.418769218 6.732882638 1.687369882 1.983584537 1.115144316 0.764787263 0.85373756 NA 1.043064166 5.824490458 1.539994138 0.765861538 1.489982827 13.7336297 Deoxyglucose NA NA NA NA NA NA NA NA NA NA 0.111897689 0.069816384 0.126528526 NA NA Deoxyguanosine 2.732550654 1.30770553 NA 1.215789375 1.572394122 NA NA NA NA NA NA 2.602958708 1.688851387 NA 0.6778398 D-Galactofuranose phosphate NA 1.418190556 NA 0.012252087 0.087368948 NA NA NA NA NA NA NA NA NA NA D-Galactose 38.80186972 0.316997165 0.197310557 0.100733579 0.627701943 0.709530982 61.49390419 43.47420193 65.09262809 0 21.49359224 4.051794685 0.959433131 0.26890341 0 D-Glucitol phosphate 0.064235399 0.080838602 NA 0.047807429 NA NA NA NA NA NA NA NA NA NA NA Diisooctyl phthalate NA 0.803079579 0.842027717 1.061463431 0.917640808 0.758559125 0.37591083 0.319717642 0.840368397 0.443826242 0.359706494 0.477833086 0.432188078 0.57112309 0.498581493 D-Mannopyranose phosphate 0.903636481 0.09138949 0.744560257 0.761067301 1.088038564 0.604983205 0.217711857 0.630817806 0.550481473 0.729052121 0.783604938 0.581113463 NA 0.207627978 NA D-Sorbitol 0.428122565 0.556362554 0.173255604 15.53063806 9.379114448 3.252019894 0.216539987 6.173363875 NA 0.814379577 5.578664977 0.138599741 38.57722253 0.56929802 0.901670867 D-Xylose NA NA NA NA NA 0.249706753 0.458494636 NA NA 2.542939819 NA NA NA NA NA D-Xylopyranose 0.713002696 0.095804035 0.473310944 0.433569695 0.044357199 0.159637415 0.023033186 0.08249099 0.141476245 0.408457536 0 1.451207075 0.91785997 0.187910866 0.445349591 Ethanimidic acid 0.50439972 0.355825074 0.28912346 NA 0.810203158 NA NA NA NA NA NA NA NA NA NA Ethyl .alpha.-D-glucopyranoside 4.986068411 NA NA 0.546735887 1.672591675 0.058127838 0.115580579 1.270738861 NA NA 1.023581956 0.014179244 0.024136619 1.087456135 0.231116991 Fumaric acid 6.062949006 0 2.241850398 1.493290838 2.440620617 3.080908074 3.020886276 2.619550519 2.975175289 2.256712972 2.924673172 3.583953789 4.343685069 2.170962826 2.147498556 Galactaric acid 6.694124963 5.189182988 0.450023659 7.354929456 11.92611519 3.714454263 2.082337858 0.812776511 7.532521706 9.636269143 6.025042732 3.662341938 1.34352295 9.786277895 14.53484276 Gluconic acid phosphate NA 1.533671305 1.199620089 2.70658299 NA 1.648876274 0.809105061 1.11899418 1.059628211 NA 3.380259069 2.302760032 0.466494973 NA NA Gluconic acid NA NA NA NA NA 0.787237441 0.039447374 0.02330581 NA NA NA NA NA NA NA Glucopyranose 22.09394773 17.24466839 29.75813105 14.18922299 29.63034761 16.00145928 21.56414127 23.71413931 31.27044789 15.99560577 15.24543636 32.91628807 4.156913682 40.86322653 53.82455418 Glyceric acid 2.699881524 0.871582728 1.296862086 1.251799753 2.096701361 1.145858883 0.592179232 0.693542016 1.688346279 0.555341372 0.68531163 2.118856935 1.70517928 0.860661365 1.306077892 Glycerol 4.31751509 2.406075405 NA NA 18.31323918 NA NA 24.48302947 48.67163847 12.1930285 34.39414185 10.07539256 64.60610686 39.54189897 38.90647892 Glycerol monostearate 0.947684061 2.028363933 0.727715903 0.84459177 0.948492662 0.836739502 0.87673773 0.631366403 1.191666877 1.307927257 0.660576959 0.48067961 0.890024502 1.305450209 3.517658795 Glycerol-3-phosphate 33.09175634 69.51188962 25.97120865 44.34222006 39.04447751 5.48773458 14.63121578 12.19402728 20.35236462 14.78787547 21.59742727 32.02875534 42.25167268 21.47689845 37.51002047 Glyceryl-glycoside 0.135340484 0.256765089 0.842027717 0.676098783 0.548943184 1.422532962 0.37591083 0.043186713 0.425400305 0.873006173 0.369001636 1.13091472 0.576024008 0.310267526 NA Glycine 24.7245338 19.80313421 11.61714332 21.84582366 25.35984876 20.23403205 17.35159598 12.8396257 19.10061299 10.55035237 18.87784683 22.42316329 18.36807121 14.69866307 13.6509357 Guanosine 13.87492664 7.409213426 NA 8.374304262 8.820722503 NA NA NA NA NA 13.74077913 14.34644844 11.14973242 NA NA Gulonic acid 4.49945223 3.922676349 1.317004345 27.91883184 4.245876028 4.080497987 1.701284655 3.170943939 NA 1.207903772 3.975140093 5.618765515 8.133098684 4.060805725 11.65959681 Heptadecanoic acid 0.190942174 0.359457357 0.251000784 NA 0.219940191 0.099311192 NA 0.115234374 NA 0.182556488 0.160928005 0.14187503 NA 0.251901935 0.672780676 Inosine 1.083589095 0.541307745 0.321325512 0.476428244 0.935799186 0.674541525 0.360764509 0.42416755 0.447112964 NA 1.32708349 1.19569679 0.715752663 0.617973682 0.550791097 L-(-)-Arabitol 2.918453994 NA 2.570788932 2.090071649 NA NA NA NA NA NA NA NA NA NA NA Lactic acid 9.757993025 1.331322571 1.75511646 1.140748985 2.178848908 4.232700534 0.708387138 1.244918429 1.406338036 1.059994776 1.083566883 3.303889359 3.06059223 0.616488105 2.646315411 L-Aspartic acid 64.24200045 43.61899899 18.76387814 31.07300821 45.74165227 52.98408746 24.82007464 44.99997892 32.15251156 18.01897892 35.97213363 33.04503784 30.52554617 29.76281502 30.76032367 L-Cystine 0.142638539 0.047195594 NA 0.117763901 0.058001185 0.064071121 0.078874789 NA 0.10225031 NA 0.092965073 NA 0.322015928 0.103999378 NA Leu-Gly NA 0.491138646 0.404695777 0.503715121 0.902880302 0.283133469 0.355734172 0.907775439 0.325144111 NA 0.915260588 NA NA 3.388739845 1.126579476 L-Fucitol 0.686477611 NA 0.505691334 0.746080048 NA NA NA NA NA NA NA NA NA NA NA L-fucose NA 1.418190556 1.110311818 0.049727442 0.902880302 NA 1.73778201 0.763925782 0.792933459 0.272990405 NA NA 1.182440305 0.601726389 9.582458214 L-Glutamic acid 10.19383239 7.106844446 14.55971876 10.78572636 37.1666069 11.53822092 8.650174768 10.62984813 16.51205228 8.964571808 9.875094834 55.48824512 21.20375096 9.762652535 11.64736016 L-Glutamine 2.128497076 75.94886121 27.98046969 0.868309733 5.695237312 65.43754662 80.810763 71.37332232 119.7239904 132.9457296 58.96793668 NA NA 5.282016095 23.92370981 L-Histidine 1.142420824 2.297634746 1.901903114 1.325145806 2.166663296 2.338574097 1.919420657 2.702796901 1.648988397 1.689815868 2.045023575 NA 4.320694368 1.619185693 1.709116806 L-Homocystine, [S-(R*,R*)]- 1.019566257 0.784350521 0.934534076 0.97819703 1.770597786 0.724956544 0.318454062 0.771102854 0.341216525 0.212582147 NA NA NA NA NA L-Homoserine NA NA NA NA NA 0.133192566 0.066250868 0.039125195 NA NA 2.246277498 NA 0.010905715 0.070627076 NA Linoleic acid 6.210373427 24.77044389 7.235160959 2.513979972 2.29234843 2.731547518 2.196640752 1.688360236 2.913321629 4.1600894 4.205685184 13.38228455 4.121723682 10.33771903 26.88198041 L-Isoleucine 16.78165609 43.85154853 8.579720801 10.78225496 1.986758157 10.24285625 6.25897534 5.483630881 10.88264117 2.889234235 16.57830815 16.28659246 14.50602289 7.232388393 8.51871425 L-Leucine 4.445351094 29.73871173 8.053224259 6.437540964 8.156756959 9.915383885 0 5.459977711 7.104437644 11.52131479 21.52649689 14.8943514 18.13841285 29.96464218 2.560844648 L-Lysine 41.16908778 20.4956433 16.97738166 18.50239812 14.16065071 22.70018209 13.94810109 12.47388081 25.03163711 8.117668243 23.31707354 NA 29.30004659 20.55316955 18.81172296 L-Methionine 0 0 0 0 0 0 0 0 0 0 0.143584004 0.860701711 0.072857533 0 0.075185812 L-Norleucine NA NA NA NA NA 0.908952673 11.16709654 NA 0.164042982 NA 0.012674231 NA 0.558369201 0.246514019 0.090425334 L-Norvaline NA 0.42147309 0.277961733 NA 0.552344194 NA NA NA NA NA 0.4296424 0.768272047 NA 0.611139372 0.163311298 L-Proline 19.55566347 18.92325095 9.163419073 17.88953046 19.1997497 9.722370249 34.14839186 9.880478065 12.97453313 11.2469817 20.41865711 19.28635232 20.46063266 13.30346718 14.30021715 L-threonine 74.77853597 18.81661246 17.71430173 23.98852003 9.935128584 18.88171262 16.12882185 13.65954971 17.29809924 10.47406735 27.73308309 43.31720607 27.79056656 16.50982632 35.68427795 L-Tryptophan 17.50445012 3.256464282 2.941199284 4.460817484 4.341533131 2.886359415 2.05334702 3.125711975 3.001894557 1.119009475 10.12779674 2.870572118 4.115879684 3.240025791 5.610223789 L-Valyl-L-leucine 1.855222875 NA NA 2.205721734 2.479708169 0.722635641 0.505078481 1.291269078 1.625216146 NA 1.284184453 NA 0.514503058 0.98245694 0.864396224 Lyxose 2.700451033 NA 1.352268201 1.739492497 2.870567779 1.720653742 0.757980354 1.462388967 1.449564709 2.523869335 0.821961499 1.320624003 1.40919083 0.068714888 1.962672673 Malic acid 9.64614329 17.01411422 18.76790515 9.9581485 11.54533001 11.17141298 7.128311074 10.93843362 8.807346872 8.423065374 8.163749083 13.66313535 9.386297834 4.61179309 5.602009258 Mannitol 16.24191274 58.64690035 142.6164751 174.4298776 55.88530585 113.9820982 136.7127902 110.2886407 112.4030702 66.13446844 83.14084493 49.5806596 38.28718501 203.2806939 41.60744638 meso-Erythritol NA NA NA NA NA 2.141261724 64.10605025 49.76094066 NA 0.248539101 1.838725742 6.001525152 NA 2.73773139 0.541170798 Methyl galactoside 1.186703268 0.877650885 0.079553671 1.29812571 1.833882364 1.722185402 1.253877802 0.974372142 0.325144111 0 0 1.996869893 1.681848387 0.778020426 2.037363593 Methyl phosphate 0.9110216 0.780279112 0.106958679 1.58557719 0.371948432 0.099242176 0.186824088 0.165037838 2.600827052 NA 1.178353883 1.4205575 0.675349628 0.508220338 0.249307956 Methylmaleic acid NA NA NA NA NA NA 0.053448247 0.022454574 0.016164532 0.175531276 0.027460832 NA 0.139727772 0.129194441 0.030876576 Monostearin 2.926997207 2.397024276 1.479485724 2.88288658 2.907555165 0.910738693 1.631495753 1.476700973 1.413263498 3.632612107 1.186547915 1.056199824 1.47723239 1.593307722 2.840050149 Myoinositol 1-phosphate 19.02614151 18.54124195 12.04011709 36.53857209 22.62240025 33.99535213 10.27623871 15.93436896 18.07987537 10.4673 15.87936838 19.04628923 31.24577187 24.37476262 23.35370955 Myo-Inositol 6.747602513 6.129567156 5.718737915 16.43618542 11.64128952 5.165864132 3.223471246 4.168459974 6.935054061 7.099186285 9.950564877 5.456926606 7.194266626 7.520446195 10.54397035 N-Acetyl-D-glucosamine 21.12111223 21.74360615 6.136768564 14.70726638 15.10735958 10.17376309 12.52909252 15.31063444 24.60209234 9.187934632 21.15027948 14.22541657 25.98065733 17.6584682 18.73059168 N-alpha-Acetyl-L-Lysine 1.855222875 0.374546065 0.228374306 1.082260127 0.89212387 NA NA NA NA NA 0.724068902 4.681509027 NA 20.72874987 NA N-ethoxycarbonyl-, nonyl ester, L-Norvaline NA NA NA NA NA 0.263220415 NA 3.69E-05 0.952840811 0.212582147 NA NA NA NA NA N-Methyl-L-prolinol NA NA NA NA NA 0.050291304 NA 0.022504756 0.002916931 NA NA NA NA NA NA N-methyl-N-methoxycarbonyl-, undecyl ester, Alanine 13.24572776 45.18453996 5.959574142 0.656501476 29.92124369 20.14806686 6.623153323 55.9525499 0.051563907 0.097249222 23.46441329 0.026245981 0.702676109 17.69030531 18.76433255 N-methoxycarbonyl-, isohexyl ester, L-Norleucine NA NA NA NA NA NA NA NA NA NA 0.000424185 0.006085202 0.003232001 0.001407815 NA Oleamide NA NA NA NA NA 0.049058206 0.067571136 NA 0.130949285 0.312609331 NA NA NA NA NA Octahydro-2,2'-Bifuran NA NA NA NA NA NA NA NA NA NA 0.009460938 NA 0.001122401 0.022129044 NA Ornithine 4.987307267 4.286945904 19.1710921 19.37755126 11.35569501 8.231290036 1.216252292 3.309939813 17.37090421 1.227157727 2.551117724 6.013885563 17.26530392 14.61855606 16.76088812 Phenylalanine 15.2313656 7.824499388 2.406957402 8.1650973 2.614792475 9.800614955 6.331823631 4.651111271 9.609524794 2.635634146 8.620255192 4.811825356 11.8116606 8.924134298 7.630810168 Palmitic Acid 3.53513355 9.010401423 4.999591652 3.084042792 1.467471848 3.507732781 2.77319235 2.510616342 3.877023798 3.784368245 3.764219851 3.636954467 3.660627968 5.84783567 9.066060019 Phenylpropanolamine 40.74128753 17.14961463 69.76305379 46.34715276 38.41203737 16.69210909 128.197257 55.75261075 23.95091135 103.0098354 38.72259592 56.70027609 0.188716084 48.61317538 23.05295768 Pipecolic acid 0.061720056 NA 23.43049454 NA 22.10883385 1.145858883 0.760035581 0.105872014 NA 59.68509909 1.032139687 32.29445068 0.213144572 0.000204113 NA Pseudo uridine 0.608864037 0.139256546 0.040872124 0.083672344 0.164269393 0.089051289 0.066883892 0.053706372 0.075027359 NA 0.107279755 0.320263792 0.314959688 0.06895255 0.043045822 Putrescine 0.536320177 1.671213521 0.257689641 1.046161756 1.138880192 2.546636239 1.137081516 1.183942508 1.822525597 1.391821077 1.861864283 0.823287272 1.39766566 2.31758515 2.513956432 Pyroglutamic acid 62.09367087 30.90850784 81.85575073 48.42559395 102.8890141 41.32919704 39.32580556 60.20197135 30.96239217 58.42676946 41.1717062 43.76370708 87.55506864 45.1427088 34.19565089 Ribitol 7.427757382 61.05584684 19.32047564 9.811455382 29.8344944 52.20968299 10.36087427 8.934633465 51.955369 96.7295372 78.98903141 99.15905282 71.63021935 6.411593495 24.01266838 Ribonic acid 1.635123521 0.188662706 0.559775465 1.211370946 1.128420471 0.599068099 0.321481653 NA 0.362671995 0.259398297 0.308556892 0.350841419 0.588289821 0.247657766 1.004924308 Serine 51.58069246 13.53386803 17.01010738 21.66112232 39.19885116 28.27665551 19.29758987 16.75936506 25.91401534 17.85212106 26.24775157 44.9699189 26.50982207 20.99394945 41.32268431 Thymidine 0.136619787 NA NA 0.041630711 0.055046694 NA NA NA NA NA NA NA NA NA NA Thymine NA NA NA NA NA NA NA NA NA NA 0.004820257 NA 0.046281532 0.003563802 0.005023086 Traumatic acid NA NA NA NA NA NA 1.202967712 3.48810666 NA 2.602169943 NA NA NA NA NA Tyramine 0.358109897 NA 0.057731596 0.125205881 0.157991242 NA NA NA NA NA 0.173439479 0.52204248 NA 0.391362277 NA Tyrosine 0.681207457 5.831352374 5.005674461 5.261606479 7.337118015 8.578149875 4.449087593 1.759605943 10.96943621 2.716311395 27.00321266 11.82327718 13.71695315 13.2385731 8.91364013 Uracil 8.015821185 3.709841022 2.090863686 0 3.964171589 3.080908074 2.504718275 1.899616966 2.975175289 2.256712972 2.924673172 12.60820287 8.645160187 2.170962826 2.147498556 Uridine 5.348109806 1.533671305 1.199620089 2.954598904 4.273292266 1.648876274 0.809105061 1.11899418 1.059628211 0.549115104 3.380259069 2.302760032 3.247976363 2.03868436 1.572467586 Uridine-5'-monophosphate 0.441939121 0.06502879 0.253652532 0.168248301 0.361486256 NA NA NA NA NA 0.092670381 0.089424349 0.063142191 NA NA Valine 18.40109524 12.48742363 9.621944856 7.568520692 9.141922087 15.07674636 14.5048832 9.900721398 11.15394538 4.443824589 14.44209635 12.20257487 9.59092806 10.42995919 20.35167824 Val-Val 0.507308894 0.374546065 0.832978514 NA NA 0.751362923 0.318454062 0.241194322 0.341216525 NA 0.512736483 NA 0.491549697 0.259686492 NA Xanthin 4.49945223 3.922676349 1.317004345 5.77038905 NA 4.080497987 1.701284655 2.451469404 1.990861032 1.207903772 NA NA NA NA NA MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name NIST ID 1-Deoxypentitol 38399-mainlib 1-Monopalmitin 37037-mainlib 1,2-Hexadecanediol 197559-mainlib 1,2,3,4-tetrahydro-6-methoxy-1-salicyl-7-Isoquinolinol 146641-mainlib 1,3-Butanediol 9344-replib 1,4-lactone, Allonic acid 38863-mainlib 1,5-Anhydro-D-sorbitol 38852-mainlib 2'-Deoxyadenosine 39426-mainlib 2-Deoxy-erythro-pentonic acid 38732-mainlib 2-deoxy-D-Erythro-Pentopyranose 38368-mainlib 2-Hydroxyglutaric acid 38518-mainlib 2-oxo-Propanoic acid 119415-mainlib 2-Phosphoglyceric acid 182213-mainlib 2-pyrrolidinone 20964-replib 2,5-Dimethoxymandelic acid 180081-mainlib 3-Deoxyhexitol 39620-mainlib 4-Aminobutanoic acid 143908-mainlib 4-Hydroxybutanoic acid 119436-mainlib 5-Hydroxypipecolic acid 182275-mainlib 5-Methyluridine 39480-mainlib 6-Fluoro-alpha-methyltryptamine 86261-mainlib Acetic acid 119424-mainlib alpha-D-(+)-Talopyranose 39379-mainlib Adenosine 39649-mainlib Adenosine-5'-monophosphate 39093-mainlib Adrenaline 86262-mainlib Ala-beta-Ala 86255-mainlib Alanine 86265-mainlib Alanylglycine 86256-mainlib alpha-D-Mannopyranose 164557-mainlib Aminomalonic acid 38864-mainlib Ammelide 204743-mainlib Asparagine 9095-replib Aucubin 37875-mainlib b-Aminoisobutyric acid 39144-mainlib Butanedioic acid 119441-mainlib cis-4-Decene-1,10-dioic acid 37794-mainlib Citric acid 191513-mainlib Cystathionine 171347-mainlib Cysteine 172585-mainlib D-(-)-Fructofuranose 39494-mainlib D-(+)-Talofuranose 170819-mainlib Deoxyglucose 39363-mainlib Deoxyguanosine 39860-mainlib D-Galactofuranose phosphate 39967-mainlib D-Galactose 9365-replib D-Glucitol phosphate 40053-mainlib Diisooctyl phthalate 21799-replib D-Mannopyranose phosphate 39404-mainlib D-Sorbitol 38923-mainlib D-Xylose 39397-mainlib D-Xylopyranose 39394-mainlib Ethanimidic acid 119576-mainlib Ethyl .alpha.-D-glucopyranoside 164509-mainlib Fumaric acid 182607-mainlib Galactaric acid 9634-replib Gluconic acid phosphate 39947-mainlib Gluconic acid 39362-mainlib Glucopyranose 39377-mainlib Glyceric acid 38809-mainlib Glycerol 39414-mainlib Glycerol monostearate 86865-mainlib Glycerol-3-phosphate 40201-mainlib Glyceryl-glycoside 164510-mainlib Glycine 24429-replib Guanosine 40113-mainlib Gulonic acid 40134-mainlib Heptadecanoic acid 38392-mainlib Inosine 39534-mainlib L-(-)-Arabitol 38768-mainlib Lactic acid 38429-mainlib L-Aspartic acid 39626-mainlib L-Cystine 171348-mainlib Leu-Gly 130516-mainlib L-Fucitol 38428-mainlib L-fucose 39381-mainlib L-Glutamic acid 182910-mainlib L-Glutamine 39000-mainlib L-Histidine 127203-mainlib L-Homocystine, [S-(R*,R*)]- 9264-replib L-Homoserine 171358-mainlib Linoleic acid 9961-replib L-Isoleucine 130529-mainlib L-Leucine 130526-mainlib L-Lysine 39149-mainlib L-Methionine 145224-mainlib L-Norleucine 39020-mainlib L-Norvaline 116925-mainlib L-Proline 9291-replib L-threonine 38447-mainlib L-Tryptophan 163567-mainlib L-Valyl-L-leucine 116931-mainlib Lyxose 39380-mainlib Malic acid 38880-mainlib Mannitol 38929-mainlib meso-Erythritol 38855-mainlib Methyl galactoside 39358-mainlib Methyl phosphate 180906-mainlib Methylmaleic acid 21394-replib Monostearin 209278-mainlib Myoinositol 1-phosphate 38857-mainlib Myo-Inositol 9617-replib N-Acetyl-D-glucosamine 142585-mainlib N-alpha-Acetyl-L-Lysine 143916-mainlib N-ethoxycarbonyl-, nonyl ester, L-Norvaline 117448-mainlib N-Methyl-L-prolinol 48768-mainlib N-methyl-N-methoxycarbonyl-, undecyl ester, Alanine 86442-mainlib N-methoxycarbonyl-, isohexyl ester, L-Norleucine 117068-mainlib Oleamide 6997-replib Octahydro-2,2'-Bifuran 35135-mainlib Ornithine 115343-mainlib Phenylalanine 39552-mainlib Palmitic Acid 86863-mainlib Phenylpropanolamine 86253-mainlib Pipecolic acid 129208-mainlib Pseudo uridine 39533-mainlib Putrescine 143937-mainlib Pyroglutamic acid 49101-mainlib Ribitol 9203-replib Ribonic acid 38766-mainlib Serine 164581-mainlib Thymidine 68930-mainlib Thymine 185875-mainlib Traumatic acid 37655-mainlib Tyramine 24434-replib Tyrosine 147068-mainlib Uracil 38117-mainlib Uridine 39489-mainlib Uridine-5'-monophosphate 39096-mainlib Valine 36240-mainlib Val-Val 116932-mainlib Xanthin 40186-mainlib METABOLITES_END #END