#METABOLOMICS WORKBENCH joerghofmann_20220406_110456 DATATRACK_ID:3177 STUDY_ID:ST002140 ANALYSIS_ID:AN003502 PROJECT_ID:PR001355
VERSION 1
CREATED_ON April 15, 2022, 1:34 pm
#PROJECT
PR:PROJECT_TITLE Mitochondrial respiration in B lymphocytes is essential for humoral immunity by
PR:PROJECT_TITLE controlling flux of the TCA cycle
PR:PROJECT_SUMMARY The function of mitochondrial respiration during B cell fate decisions and
PR:PROJECT_SUMMARY differentiation 55 remained equivocal. This study reveals that selection for
PR:PROJECT_SUMMARY mitochondrial fitness occurs during B 56 cell activation and is essential for
PR:PROJECT_SUMMARY subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial
PR:PROJECT_SUMMARY helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell
PR:PROJECT_SUMMARY maturation, resulting in reduced oxidative phosphorylation. Although no changes
PR:PROJECT_SUMMARY in 59 follicular B cell development were evident, germinal centers, class switch
PR:PROJECT_SUMMARY recombination to 60 IgG, plasma cell maturation and humoral immunity were
PR:PROJECT_SUMMARY diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the
PR:PROJECT_SUMMARY tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic
PR:PROJECT_SUMMARY acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas
PR:PROJECT_SUMMARY HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased
PR:PROJECT_SUMMARY mTOR activity in activated B cells. Hence, mitochondrial function is required
PR:PROJECT_SUMMARY and 65 selected for in activated B cells for the successful generation of
PR:PROJECT_SUMMARY functional plasma cells.
PR:INSTITUTE University of Erlangen-Nuremberg Chair of Biochemistry
PR:LAST_NAME Hofmann
PR:FIRST_NAME Joerg
PR:ADDRESS Staudtstr.5, Erlangen, Bavaria, 91058, Germany
PR:EMAIL joerg.hofmann@fau.de
PR:PHONE +49 9131 85 8260
#STUDY
ST:STUDY_TITLE Mitochondrial respiration in B lymphocytes is essential for humoral immunity by
ST:STUDY_TITLE controlling flux of the TCA cycle
ST:STUDY_SUMMARY The function of mitochondrial respiration during B cell fate decisions and
ST:STUDY_SUMMARY differentiation 55 remained equivocal. This study reveals that selection for
ST:STUDY_SUMMARY mitochondrial fitness occurs during B 56 cell activation and is essential for
ST:STUDY_SUMMARY subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial
ST:STUDY_SUMMARY helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell
ST:STUDY_SUMMARY maturation, resulting in reduced oxidative phosphorylation. Although no changes
ST:STUDY_SUMMARY in 59 follicular B cell development were evident, germinal centers, class switch
ST:STUDY_SUMMARY recombination to 60 IgG, plasma cell maturation and humoral immunity were
ST:STUDY_SUMMARY diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the
ST:STUDY_SUMMARY tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic
ST:STUDY_SUMMARY acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas
ST:STUDY_SUMMARY HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased
ST:STUDY_SUMMARY mTOR activity in activated B cells. Hence, mitochondrial function is required
ST:STUDY_SUMMARY and 65 selected for in activated B cells for the successful generation of
ST:STUDY_SUMMARY functional plasma cells.
ST:INSTITUTE University of Erlangen-Nürnberg
ST:DEPARTMENT Division of Molecular Immunology.Universitätsklinikum Erlangen, Nikolaus
ST:DEPARTMENT Fibinger Zentrum
ST:LABORATORY Prof. Mielenz
ST:LAST_NAME Mielenz
ST:FIRST_NAME Dirk
ST:ADDRESS Nikolaus-Fiebiger-Zentrum, Glückstraße 6, 91054 Erlangen
ST:EMAIL dirk.mielenz@fau.de
ST:PHONE ++49 9131 8539105
#SUBJECT
SU:SUBJECT_TYPE Cultured cells
SU:SUBJECT_SPECIES Mus musculus
SU:TAXONOMY_ID 10090
SU:GENDER Male and female
#FACTORS
#SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS - 1aCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=1aCre_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 2aCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=2aCre_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 3aDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=3aDNT_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 4aDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=4aDNT_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 1bCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=1bCre_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 2bCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=2bCre_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 3bDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=3bDNT_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 4bDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=4bDNT_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 1cCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=1cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 2cCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=2cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 3cCre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=3cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 4cDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=4cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 5cDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=5cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - 6cDNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=6cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S01_DNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S01_DNT.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S02_Cre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S02_Cre.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S03_DNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S03_DNT.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S04_Cre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S04_Cre.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S05_DNT Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S05_DNT.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 1-S06_Cre Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S06_Cre.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S01_Cre1 Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S01_Cre1.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S02_DNT2 Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S02_DNT2.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S03_Cre3 Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S03_Cre3.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S04_DNT4 Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S04_DNT4.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S05_Cre5 Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S05_Cre5.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S06_DNT6 Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S06_DNT6.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S07_Cre7 Genotype:wildtype Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S07_Cre7.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S08_DNT8 Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S08_DNT8.mzML
SUBJECT_SAMPLE_FACTORS - B cells_DNT_GPL analysis 2b-S10_DNT10 Genotype:mutant Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S10_DNT10.mzML
#COLLECTION
CO:COLLECTION_SUMMARY Both female and male mice were used in the experiments. Mice were maintained on
CO:COLLECTION_SUMMARY a 12-h light/dark cycle with free access to food and water according to
CO:COLLECTION_SUMMARY governmental rules. K320E-TWINKLE floxed mice (Baris et al., 2015) were crossed
CO:COLLECTION_SUMMARY to CD23 CRE mice (Kwon et al., 2008) kindly provided by Meinrad Busslinger) to
CO:COLLECTION_SUMMARY generate DNT animals. DNT mice used in these experiments had the genetic
CO:COLLECTION_SUMMARY background DNT+/- CRE+/- and CRE control mice were DNT-/- CRE+/-. The WT animals
CO:COLLECTION_SUMMARY used in this study were DNT-/- CRE-/- littermates. All mice were on the C57Bl/6
CO:COLLECTION_SUMMARY background. Isolation of primary murine cells from spleen and bone marrow Spleen
CO:COLLECTION_SUMMARY was transferred into cold 2 % FCS (in PBS) and gently passed through a 70 µm
CO:COLLECTION_SUMMARY cell strainer (BD) using the plunger of a 5 ml syringe (BD). Femur and tibia
CO:COLLECTION_SUMMARY were flushed with cold 2 % FCS using a 27 G cannula (BD). Cell suspensions were
CO:COLLECTION_SUMMARY pelleted by centrifugation at 300 x g for 5 min at 4°C. Erythrocytes were lysed
CO:COLLECTION_SUMMARY in red blood cell-lysis buffer (150 mM NH4Cl, 10 mM KHCO3, 100 µM EDTA) for
CO:COLLECTION_SUMMARY 5min at room temperature. The reaction was stopped by adding cold 2% FCS before
CO:COLLECTION_SUMMARY centrifugation at 300 x g for 5 min at 4°C. The final cell suspensions were
CO:COLLECTION_SUMMARY kept in cold 2 % FCS after filtration through 30 µm mesh filter (Sysmex). In
CO:COLLECTION_SUMMARY vitro cultivation of primary murine B cells Splenic B cells were cultured with a
CO:COLLECTION_SUMMARY starting concentration of 0.5 x 106 cells/ ml in R10 medium (RPMI1640, 10 %
CO:COLLECTION_SUMMARY fetal calf serum (FCS), 2 mM glutamate, 1 mM sodium pyruvate, 50 U/ml penicillin
CO:COLLECTION_SUMMARY G, 50 μg/ml streptomycin, 50 μM β-mercaptoethanol) for 72 h at 37°C and 5%
CO:COLLECTION_SUMMARY CO2, supplemented with 10 µg/ml LPS. For in vitro class switch recombination
CO:COLLECTION_SUMMARY cells were seeded at 0.1 x 106 cells/ ml in R10 medium for 96 h, supplemented
CO:COLLECTION_SUMMARY with 5 ng/ml transforming growth factor , 5 nM retinoic acid, 10 µg/ml
CO:COLLECTION_SUMMARY anti-CD40 antibody, 10 µg/ml LPS, 100 U/ml IL4 and 10 ng/ml IL5. Ref.: Baris,
CO:COLLECTION_SUMMARY O.R., Ederer, S., Neuhaus, J.F., von Kleist-Retzow, J.C., Wunderlich, C.M., Pal,
CO:COLLECTION_SUMMARY M., WunderlichF.T., Peeva, V., Zsurka, G., Kunz, W.S., et al. (2015). Mosaic
CO:COLLECTION_SUMMARY Deficiency in Mitochondrial Oxidative Metabolism Promotes Cardiac Arrhythmia
CO:COLLECTION_SUMMARY during Aging. Cell Metab 21, 667–677.
CO:COLLECTION_PROTOCOL_FILENAME Bcellscoll Mielenz.pdf
CO:SAMPLE_TYPE B-cells
#TREATMENT
TR:TREATMENT_SUMMARY in vitro activated (LPS) B cells, which cannot replicate their mitochondrial DNA
TR:TREATMENT_SUMMARY ("DNTwinkle") resulting in impaired respiratory chain activity and oxidative
TR:TREATMENT_SUMMARY phosphorylation compared to Cre control B cells DTN Dominant Negative Twinkle
TR:TREATMENT_SUMMARY (mitochondtail Helicase)
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY Glycerophospholipid analysis Glycerophospholipids (PCH, PE, PI, PS, PG, PA) in B
SP:SAMPLEPREP_SUMMARY cells were analyzed by Nano- Electrospray Ionization Tandem Mass Spectrometry
SP:SAMPLEPREP_SUMMARY (Nano-ESI-MS/MS) with direct infusion of the lipid extract (Shotgun Lipidomics):
SP:SAMPLEPREP_SUMMARY 14 to 45 x 106 cells were homogenized in 300 μl of Milli- Q water using the
SP:SAMPLEPREP_SUMMARY Precellys 24 Homogenisator (Peqlab, Erlangen, Germany) at 6.500 rpm for 30 sec.
SP:SAMPLEPREP_SUMMARY The protein content of the homogenate was routinely determined using
SP:SAMPLEPREP_SUMMARY bicinchoninic acid. To 100 μl of the homogenate 400 μl of Milli-Q water,
SP:SAMPLEPREP_SUMMARY 1.875ml of methanol/chloroform 2:1 (v/v) and internal standards (125 pmol PCH
SP:SAMPLEPREP_SUMMARY 17:0-20:4, 132 pmol PE 17:0-20:4, 118 pmol PI 17:0-20:4, 131 pmol PS 17:0-20:4,
SP:SAMPLEPREP_SUMMARY 62 pmol PG 17:0/20:4, 75 pmol PA 17:0/20:4 Avanti Polar Lipids) were added.
SP:SAMPLEPREP_SUMMARY Lipid extraction and Nano-ESI-MS/MS analysis were performed as previously
SP:SAMPLEPREP_SUMMARY described (Kumar et al., 2015). Endogenous glycerophospolipids were quantified
SP:SAMPLEPREP_SUMMARY by referring their peak areas to those of the internal standards. The calculated
SP:SAMPLEPREP_SUMMARY glycerophospolipid amounts were normalized to the protein content of the tissue
SP:SAMPLEPREP_SUMMARY homogenate. Metabolomics of phosphorylated metabolites und carbonic acids
SP:SAMPLEPREP_SUMMARY Experimental Setup I: Splenic B cells were isolated, activated with LPS and
SP:SAMPLEPREP_SUMMARY viable cells, only GFP+ for DNT, were sorted after 3 days using flow cytometry.
SP:SAMPLEPREP_SUMMARY Perchloric acid extraction and metabolic profiling was performed as previously
SP:SAMPLEPREP_SUMMARY published measured by LCMS/MS on an QTrap 3200 (Sciex) (Hofmann et al., 2011).
SP:SAMPLEPREP_SUMMARY Ref.: Kumar, V., Bouameur, J.E., Bar, J., Rice, R.H., Hornig-Do, H.T., Roop,
SP:SAMPLEPREP_SUMMARY D.R., Schwarz, N., Brodesser, 1120 S., Thiering, S., Leube, R.E., et al. (2015).
SP:SAMPLEPREP_SUMMARY A keratin scaffold regulates epidermal barrier 1121 formation, mitochondrial
SP:SAMPLEPREP_SUMMARY lipid composition, and activity. J Cell Biol 211, 1057–1075. Ref.: Hofmann,
SP:SAMPLEPREP_SUMMARY J., Bornke, F., Schmiedl, A., Kleine, T., and Sonnewald, U. (2011). Detecting
SP:SAMPLEPREP_SUMMARY functional groups of Arabidopsis mutants by metabolic profiling and evaluation
SP:SAMPLEPREP_SUMMARY of pleiotropic responses. 10Front Plant Sci 2, 82.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY Dried lipid extracts were dissolved in 300 μl of methanol. 20 μl of the lipid
CH:CHROMATOGRAPHY_SUMMARY extract in methanol were loaded into 96-well plates and diluted with 20 μl of
CH:CHROMATOGRAPHY_SUMMARY 20 mM ammonium acetate in methanol. Lipid infusion and ionization was conducted
CH:CHROMATOGRAPHY_SUMMARY using Nano-ESI chips with the TriVersa NanoMate operated by the ChipSoft
CH:CHROMATOGRAPHY_SUMMARY Software (Advion) under the following settings: sample infusion volume: 14 μl,
CH:CHROMATOGRAPHY_SUMMARY volume of air to aspirate after sample: 1 μl, air gap before chip: enabled,
CH:CHROMATOGRAPHY_SUMMARY aspiration delay: 0 s, prepiercing: with mandrel, spray sensing: enabled,
CH:CHROMATOGRAPHY_SUMMARY cooling temperature: 14°C, gas pressure: 0.5 psi, ionization voltage: 1.4 kV,
CH:CHROMATOGRAPHY_SUMMARY and vent headspace: enabled. Prewetting was done once. Ref.: Kumar, V.,
CH:CHROMATOGRAPHY_SUMMARY Bouameur, J.E., Bar, J., Rice, R.H., Hornig-Do, H.T., Roop, D.R., Schwarz, N.,
CH:CHROMATOGRAPHY_SUMMARY Brodesser, 1120 S., Thiering, S., Leube, R.E., et al. (2015). A keratin scaffold
CH:CHROMATOGRAPHY_SUMMARY regulates epidermal barrier 1121 formation, mitochondrial lipid composition, and
CH:CHROMATOGRAPHY_SUMMARY activity. J Cell Biol 211, 1057–1075. Ref.: Kumar, V., Bouameur, J.E., Bar,
CH:CHROMATOGRAPHY_SUMMARY J., Rice, R.H., Hornig-Do, H.T., Roop, D.R., Schwarz, N., Brodesser, 1120 S.,
CH:CHROMATOGRAPHY_SUMMARY Thiering, S., Leube, R.E., et al. (2015). A keratin scaffold regulates epidermal
CH:CHROMATOGRAPHY_SUMMARY barrier 1121 formation, mitochondrial lipid composition, and activity. J Cell
CH:CHROMATOGRAPHY_SUMMARY Biol 211, 1057–1075.
CH:CHROMATOGRAPHY_TYPE None (Direct infusion)
CH:INSTRUMENT_NAME TriVersa NanoMate
CH:COLUMN_NAME TriVersa NanoMate
#ANALYSIS
AN:ANALYSIS_TYPE MS
#MS
MS:INSTRUMENT_NAME ABI Sciex 6500 QTrap
MS:INSTRUMENT_TYPE QTRAP
MS:MS_TYPE ESI
MS:ION_MODE POSITIVE
MS:MS_COMMENTS Mass spectrometric analysis was performed using the QTRAP 6500 (SCIEX) operated
MS:MS_COMMENTS by Analyst 1.6.3. The following instrument dependent settings were used: curtain
MS:MS_COMMENTS gas, 20 psi; CAD gas, medium; and interface heater temperature, 100°C. PC
MS:MS_COMMENTS analysis was performed in the positive ion mode by scanning for precursors of
MS:MS_COMMENTS m/z 184 at a collision energy of 35 eV. PE, PS, PG, PI, and PA measurements were
MS:MS_COMMENTS performed in the positive ion mode by scanning for neutral losses of 141, 185,
MS:MS_COMMENTS 189, 277, and 115 D at CE of 25 eV. The value for the declustering potential was
MS:MS_COMMENTS 100 V (Özbalci et al. (2013) Methods Mol Biol 1033:3-20). Scanning was
MS:MS_COMMENTS performed in a mass range of m/z 650–900 D and at a scan rate of 200 D/s. 61
MS:MS_COMMENTS MCA spectra were accumulated. Mass spectra were processed by the LipidView
MS:MS_COMMENTS Software Version 1.2 (SCIEX) for identification and quantification of
MS:MS_COMMENTS glycerophospholipids. Endogenous glycerophospolipids were quantified by
MS:MS_COMMENTS referring their peak areas to those of the internal standards.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS mol%
MS_METABOLITE_DATA_START
Samples B cells_DNT_GPL analysis 1-S01_DNT B cells_DNT_GPL analysis 1-S02_Cre B cells_DNT_GPL analysis 1-S03_DNT B cells_DNT_GPL analysis 1-S04_Cre B cells_DNT_GPL analysis 1-S05_DNT B cells_DNT_GPL analysis 1-S06_Cre B cells_DNT_GPL analysis 2b-S01_Cre1 B cells_DNT_GPL analysis 2b-S02_DNT2 B cells_DNT_GPL analysis 2b-S03_Cre3 B cells_DNT_GPL analysis 2b-S04_DNT4 B cells_DNT_GPL analysis 2b-S05_Cre5 B cells_DNT_GPL analysis 2b-S06_DNT6 B cells_DNT_GPL analysis 2b-S07_Cre7 B cells_DNT_GPL analysis 2b-S08_DNT8 B cells_DNT_GPL analysis 2b-S10_DNT10
Factors Genotype:mutant Genotype:wildtype Genotype:mutant Genotype:wildtype Genotype:mutant Genotype:wildtype Genotype:wildtype Genotype:mutant Genotype:wildtype Genotype:mutant Genotype:wildtype Genotype:mutant Genotype:wildtype Genotype:mutant Genotype:mutant
Phosphatidylcholine_30:0 5 4 5 4 5 4 3 5 3 5 3 5 3 4 5
Phosphatidylcholine_32:0 9 8 9 8 10 7 8 11 8 11 7 11 6 10 10
Phosphatidylcholine_32:1 15 14 14 16 14 15 14 15 16 14 16 15 15 14 15
Phosphatidylcholine_32:2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylcholine_32:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_34:0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Phosphatidylcholine_34:1 21 22 21 23 20 23 24 22 23 21 22 20 22 21 20
Phosphatidylcholine_34:2 6 6 6 7 7 8 7 7 8 6 8 6 7 6 6
Phosphatidylcholine_34:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_34:4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_36:0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_36:1 3 4 3 4 3 3 3 2 3 3 3 2 3 3 3
Phosphatidylcholine_36:2 8 10 9 10 8 10 10 7 10 8 11 7 11 9 8
Phosphatidylcholine_36:3 1 1 2 1 2 1 1 1 1 1 1 1 1 1 1
Phosphatidylcholine_36:4 1 1 1 1 1 1 1 1 1 1 0 1 0 1 1
Phosphatidylcholine_36:5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_36:6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_38:2 1 1 1 1 1 1 1 0 1 1 1 1 1 1 1
Phosphatidylcholine_38:3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_38:4 1 0 1 1 1 1 0 1 0 1 0 1 0 0 1
Phosphatidylcholine_38:5 1 1 1 1 1 1 1 1 0 1 0 1 0 1 1
Phosphatidylcholine_38:6 0 0 1 0 1 0 0 1 0 0 0 1 0 0 1
Phosphatidylcholine_38:7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylcholine_40:9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylethanolamine_32:0 2 1 1 1 2 1 1 2 1 2 1 2 1 1 1
Phosphatidylethanolamine_32:1 6 6 5 6 5 6 5 5 6 4 7 5 7 6 5
Phosphatidylethanolamine_34:1 11 14 10 13 10 14 14 10 15 10 16 10 17 13 11
Phosphatidylethanolamine_34:2 5 5 4 5 4 5 5 4 6 4 6 4 6 6 4
Phosphatidylethanolamine_34:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylethanolamine_36:0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylethanolamine_36:1 3 5 3 5 3 5 5 3 5 3 6 3 6 5 3
Phosphatidylethanolamine_36:2 7 9 7 8 7 9 9 6 10 7 10 7 10 10 7
Phosphatidylethanolamine_36:3 2 2 3 2 3 2 2 3 2 3 2 3 2 3 3
Phosphatidylethanolamine_36:4 6 5 6 5 6 5 6 6 5 6 4 6 3 4 6
Phosphatidylethanolamine_36:5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylethanolamine_36:6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylethanolamine_38:0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylethanolamine_38:1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Phosphatidylethanolamine_38:2 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0
Phosphatidylethanolamine_38:3 2 1 2 1 2 1 2 2 2 2 1 2 1 1 2
Phosphatidylethanolamine_38:4 13 11 14 11 14 10 11 15 10 14 9 14 9 10 14
Phosphatidylethanolamine_38:5 8 7 8 7 8 6 7 8 6 8 6 8 4 5 8
Phosphatidylethanolamine_38:6 5 4 5 4 5 4 4 5 4 5 3 5 3 3 5
Phosphatidylethanolamine_38:7 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Phosphatidylethanolamine_40:4 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylethanolamine_40:5 3 3 3 3 3 3 3 3 2 3 3 3 2 2 3
Phosphatidylethanolamine_40:6 6 5 7 6 7 6 5 7 5 7 5 7 4 4 7
Phosphatidylethanolamine_40:7 3 2 3 2 3 2 2 3 2 3 2 3 1 2 3
Phosphatidylethanolamine_40:8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylethanolamine_40:9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylinositol_32:0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Phosphatidylinositol_32:1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1
Phosphatidylinositol_32:2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylinositol_34:1 7 10 6 9 6 10 13 8 14 9 13 7 13 9 8
Phosphatidylinositol_34:2 4 3 3 3 3 4 4 4 4 4 4 4 3 3 4
Phosphatidylinositol_34:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylinositol_36:1 12 17 10 15 9 17 19 10 22 11 21 10 23 16 10
Phosphatidylinositol_36:2 22 23 19 22 19 24 23 19 24 20 26 19 24 24 20
Phosphatidylinositol_36:3 3 2 3 2 3 2 2 3 2 3 2 3 2 2 3
Phosphatidylinositol_36:4 3 2 3 2 3 2 2 4 2 4 2 4 2 3 4
Phosphatidylinositol_36:5 1 0 1 0 1 0 0 1 0 1 0 1 0 0 1
Phosphatidylinositol_38:1 0 1 0 1 0 1 1 0 1 0 1 0 1 1 0
Phosphatidylinositol_38:2 2 3 2 3 2 3 3 2 3 2 3 2 3 3 2
Phosphatidylinositol_38:3 6 6 7 6 8 6 6 6 4 6 5 6 5 6 7
Phosphatidylinositol_38:4 22 17 27 19 25 17 15 24 11 20 12 24 14 20 22
Phosphatidylinositol_38:5 10 7 11 8 12 7 5 11 5 9 5 11 4 7 11
Phosphatidylinositol_38:6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidylinositol_40:2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylinositol_40:3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylinositol_40:5 2 2 2 2 2 2 2 2 1 2 1 2 1 2 2
Phosphatidylinositol_40:6 2 2 2 2 2 2 2 2 1 2 1 2 1 1 2
Phosphatidylinositol_40:7 1 1 1 1 1 1 0 1 0 0 0 1 0 0 1
Phosphatidylinositol_40:9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Phosphatidylserine_32:3 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Phosphatidylserine_36:4 1 1 1 1 1 1 2 2 1 2 2 1 1 1 1
Phosphatidylserine_38:1 1 1 1 1 1 1 1 1 2 1 1 1 2 1 1
Phosphatidylserine_38:3 5 4 6 5 5 4 6 8 6 8 6 8 3 7 8
Phosphatidylserine_38:4 5 5 5 5 5 4 6 7 6 6 6 7 4 7 7
Phosphatidylserine_38:5 3 3 3 3 3 2 3 4 3 4 3 4 3 2 4
Phosphatidylserine_38:6 3 2 2 2 2 2 2 3 2 2 2 3 2 2 3
Phosphatidylserine_40:1 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0
Phosphatidylserine_40:2 0 0 0 0 0 0 1 0 1 0 1 0 1 1 0
Phosphatidylserine_40:3 0 0 0 0 0 0 0 1 1 1 1 1 0 0 1
Phosphatidylserine_40:4 4 3 4 3 4 3 4 6 3 5 4 7 4 6 6
Phosphatidylserine_40:5 16 10 15 12 15 10 13 20 13 17 13 21 13 14 19
Phosphatidylserine_40:6 19 12 18 14 18 11 14 21 14 20 13 22 15 18 21
Phosphatidylserine_40:7 2 1 2 1 2 1 1 2 1 1 1 2 2 2 2
Phosphatidylglycerol_32:0 3 3 3 3 3 3 3 4 5 5 9 3 3 6 4
Phosphatidylglycerol_32:1 5 4 4 4 5 4 6 5 3 5 5 4 4 5 5
Phosphatidylglycerol_32:2 1 1 1 1 1 1 0 1 1 1 1 1 1 1 1
Phosphatidylglycerol_34:1 65 61 60 66 61 64 52 63 59 42 52 64 66 45 62
Phosphatidylglycerol_34:2 6 5 5 5 6 5 5 7 5 4 6 5 6 6 6
Phosphatidylglycerol_36:1 6 11 8 8 7 9 16 5 9 14 11 6 8 14 6
Phosphatidylglycerol_36:2 7 7 8 6 8 7 9 7 6 10 8 8 7 11 7
Phosphatidylglycerol_36:3 2 1 2 2 2 1 1 2 1 2 2 3 2 2 2
Phosphatidylglycerol_36:4 1 1 1 1 1 1 1 2 3 3 1 2 1 2 2
Phosphatidylglycerol_38:2 1 1 1 0 1 0 1 0 1 2 1 1 1 1 1
Phosphatidylglycerol_38:3 1 1 1 1 1 0 2 1 1 4 1 1 0 2 1
Phosphatidylglycerol_38:4 1 1 1 1 1 1 2 1 2 4 1 1 1 2 1
Phosphatidylglycerol_38:5 1 1 1 0 1 0 1 1 1 2 1 1 1 1 1
Phosphatidic acid_30:1 3 1 3 1 2 1 1 2 1 1 1 2 1 1 1
Phosphatidic acid_30:2 1 0 1 0 0 0 1 1 0 1 0 1 0 0 0
Phosphatidic acid_32:0 29 38 28 36 26 35 32 17 26 11 27 17 28 14 17
Phosphatidic acid_32:1 14 7 13 8 12 8 7 11 9 9 8 11 7 8 11
Phosphatidic acid_32:2 2 1 2 2 2 1 2 2 1 1 2 2 1 2 2
Phosphatidic acid_34:0 4 10 3 8 4 9 9 4 5 3 6 3 6 3 3
Phosphatidic acid_34:1 19 20 21 20 22 22 17 21 23 20 22 22 24 26 21
Phosphatidic acid_34:2 5 5 6 6 7 5 5 8 5 5 5 7 5 6 7
Phosphatidic acid_34:3 1 0 1 0 1 0 0 1 0 1 1 1 0 1 1
Phosphatidic acid_36:0 1 1 1 1 1 1 3 2 2 2 2 2 2 2 2
Phosphatidic acid_36:1 3 4 4 4 4 4 5 6 9 10 6 7 6 10 7
Phosphatidic acid_36:2 5 5 5 5 6 6 6 8 8 11 7 8 8 10 8
Phosphatidic acid_36:3 1 1 1 1 1 1 1 2 1 2 1 2 1 2 2
Phosphatidic acid_36:4 1 0 1 0 1 0 1 2 1 2 1 2 1 1 2
Phosphatidic acid_36:5 1 0 1 0 0 0 0 1 0 1 1 1 1 1 1
Phosphatidic acid_38:2 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1
Phosphatidic acid_38:4 1 0 1 0 1 0 1 2 1 3 1 2 1 2 2
Phosphatidic acid_38:5 1 0 1 1 1 1 1 2 1 2 1 2 1 1 2
Phosphatidic acid_38:6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidic acid_38:7 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidic acid_40:5 1 0 1 0 0 0 1 3 1 4 1 2 1 2 2
Phosphatidic acid_40:6 1 0 1 0 1 0 1 2 1 4 1 3 1 2 2
Phosphatidic acid_40:7 1 1 1 1 1 1 1 1 1 1 1 1 0 1 1
Phosphatidic acid_40:8 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Phosphatidic acid_40:9 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name CAS number Mass to Charge Fragmentation Modification Charge
Phosphatidylcholine_30:0 8002-43-5 706,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_32:0 8002-43-5 734,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_32:1 8002-43-5 732,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_32:2 8002-43-5 730,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_32:3 8002-43-5 728,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_34:0 8002-43-5 762,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_34:1 8002-43-5 760,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_34:2 8002-43-5 758,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_34:3 8002-43-5 756,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_34:4 8002-43-5 754,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:0 8002-43-5 790,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:1 8002-43-5 788,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:2 8002-43-5 786,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:3 8002-43-5 784,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:4 8002-43-5 782,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:5 8002-43-5 780,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_36:6 8002-43-5 778,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:2 8002-43-5 814,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:3 8002-43-5 812,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:4 8002-43-5 810,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:5 8002-43-5 808,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:6 8002-43-5 806,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_38:7 8002-43-5 804,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:3 8002-43-5 840,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:4 8002-43-5 838,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:5 8002-43-5 836,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:6 8002-43-5 834,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:7 8002-43-5 832,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:8 8002-43-5 830,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylcholine_40:9 8002-43-5 828,5 +Precursor Ion Scan m/z 184 [M+H] 1
Phosphatidylethanolamine_32:0 39382-08-6 692,4 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_32:1 39382-08-6 690,4 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_34:1 39382-08-6 718,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_34:2 39382-08-6 716,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_34:3 39382-08-6 714,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:0 39382-08-6 748,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:1 39382-08-6 746,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:2 39382-08-6 744,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:3 39382-08-6 742,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:4 39382-08-6 740,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:5 39382-08-6 738,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_36:6 39382-08-6 736,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:0 39382-08-6 776,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:1 39382-08-6 774,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:2 39382-08-6 772,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:3 39382-08-6 770,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:4 39382-08-6 768,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:5 39382-08-6 766,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:6 39382-08-6 764,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_38:7 39382-08-6 762,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:4 39382-08-6 796,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:5 39382-08-6 794,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:6 39382-08-6 792,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:7 39382-08-6 790,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:8 39382-08-6 788,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylethanolamine_40:9 39382-08-6 786,5 +Neutral Loss Scan m/z 141 [M+H] 1
Phosphatidylinositol_32:0 383907-36-6 828,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_32:1 383907-36-6 826,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_32:2 383907-36-6 824,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_34:1 383907-36-6 854,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_34:2 383907-36-6 852,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_34:3 383907-36-6 850,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_36:1 383907-36-6 882,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_36:2 383907-36-6 880,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_36:3 383907-36-6 878,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_36:4 383907-36-6 876,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_36:5 383907-36-6 874,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:1 383907-36-6 910,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:2 383907-36-6 908,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:3 383907-36-6 906,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:4 383907-36-6 904,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:5 383907-36-6 902,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_38:6 383907-36-6 900,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:2 383907-36-6 936,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:3 383907-36-6 934,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:5 383907-36-6 930,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:6 383907-36-6 928,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:7 383907-36-6 926,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylinositol_40:9 383907-36-6 922,5 +Neutral Loss Scan m/z 277 [M+NH4] 1
Phosphatidylserine_32:3 383907-32-2 730,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_36:4 383907-32-2 784,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_38:1 383907-32-2 818,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_38:3 383907-32-2 814,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_38:4 383907-32-2 812,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_38:5 383907-32-2 810,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_38:6 383907-32-2 808,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:1 383907-32-2 846,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:2 383907-32-2 844,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:3 383907-32-2 842,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:4 383907-32-2 840,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:5 383907-32-2 838,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:6 383907-32-2 836,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylserine_40:7 383907-32-2 834,5 +Neutral Loss Scan m/z 185 [M+H] 1
Phosphatidylglycerol_32:0 383907-64-0 740,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_32:1 383907-64-0 738,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_32:2 383907-64-0 736,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_34:1 383907-64-0 766,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_34:2 383907-64-0 764,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_36:1 383907-64-0 794,5 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_36:2 383907-64-0 792,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_36:3 383907-64-0 790,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_36:4 383907-64-0 788,4 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_38:2 383907-64-0 820,5 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_38:3 383907-64-0 818,5 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_38:4 383907-64-0 816,5 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidylglycerol_38:5 383907-64-0 814,5 +Neutral Loss Scan m/z 189 [M+NH4] 1
Phosphatidic acid_30:1 383907-53-7 636,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_30:2 383907-53-7 634,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_32:0 383907-53-7 666,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_32:1 383907-53-7 664,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_32:2 383907-53-7 662,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_34:0 383907-53-7 694,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_34:1 383907-53-7 692,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_34:2 383907-53-7 690,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_34:3 383907-53-7 688,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:0 383907-53-7 722,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:1 383907-53-7 720,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:2 383907-53-7 718,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:3 383907-53-7 716,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:4 383907-53-7 714,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_36:5 383907-53-7 712,4 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_38:2 383907-53-7 746,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_38:4 383907-53-7 742,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_38:5 383907-53-7 740,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_38:6 383907-53-7 738,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_38:7 383907-53-7 736,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_40:5 383907-53-7 768,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_40:6 383907-53-7 766,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_40:7 383907-53-7 764,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_40:8 383907-53-7 762,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
Phosphatidic acid_40:9 383907-53-7 760,5 +Neutral Loss Scan m/z 115 [M+NH4] 1
METABOLITES_END
#END