#METABOLOMICS WORKBENCH jimenezsousa_20220609_081253 DATATRACK_ID:3299 STUDY_ID:ST002194 ANALYSIS_ID:AN003591 VERSION 1 CREATED_ON 06-14-2022 #PROJECT PR:PROJECT_TITLE Metabolic profiling at COVID-19 onset shows disease severity and sex-specific PR:PROJECT_TITLE dysregulation PR:PROJECT_SUMMARY Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes an acute PR:PROJECT_SUMMARY respiratory disease, the coronavirus disease 2019 (COVID-19), which has been PR:PROJECT_SUMMARY associated with high mortality and morbidity rates worldwide. However, the PR:PROJECT_SUMMARY biological mechanisms of SARS-CoV-2 induced pathology are still not completely PR:PROJECT_SUMMARY understood. This study aimed to characterize the metabolic and cytokine profile PR:PROJECT_SUMMARY of COVID-19 patients at hospital admission and identify its association with the PR:PROJECT_SUMMARY highest COVID-19 severity reached, as well as the differences by sex. We PR:PROJECT_SUMMARY performed a metabolomics analysis in 129 COVID-19 patients enrolled from March PR:PROJECT_SUMMARY to September 2020 at three hospitals in Madrid: Infanta Leonor University PR:PROJECT_SUMMARY Hospital, del Tajo University Hospital, and Príncipe de Asturias University PR:PROJECT_SUMMARY Hospital (although 123 fulfilled criteria and were used in statistical PR:PROJECT_SUMMARY analysis), and a control group of 15 pre-pandemic healthy controls without any PR:PROJECT_SUMMARY known infection. Patient samples were collected at hospital admission or within PR:PROJECT_SUMMARY the first days after hospitalization and an untargeted plasma metabolic PR:PROJECT_SUMMARY profiling (gas chromatography and capillary electrophoresis-mass spectrometry PR:PROJECT_SUMMARY (GC and CE-MS)) was performed. The primary outcomes were SARS-CoV-2 infection PR:PROJECT_SUMMARY (COVID-19 patients versus healthy controls), COVID-19 symptomatology PR:PROJECT_SUMMARY (hospitalized symptomatic patients (moderate plus severe) versus asymptomatic), PR:PROJECT_SUMMARY and COVID-19 severe (severe versus moderate). PR:INSTITUTE CEMBIO PR:LAST_NAME Barbas PR:FIRST_NAME Coral PR:ADDRESS CEU Universities, Urbanización Montepríncipe PR:EMAIL cbarbas@ceu.es PR:PHONE +34 913724700 PR:DOI http://dx.doi.org/10.21228/M8141Z #STUDY ST:STUDY_TITLE Metabolic profiling at COVID-19 onset shows disease severity and sex-specific ST:STUDY_TITLE dysregulation ST:STUDY_SUMMARY In this study CE-MS and GC-MS based metabolomics was used to analyze COVID-19 ST:STUDY_SUMMARY disease. In total, 144 individuals classified as healthy, asymptomatic/mils, ST:STUDY_SUMMARY moderate and severe according to the highest COVID-19 severity status, were ST:STUDY_SUMMARY analyzed. ST:INSTITUTE CEMBIO ST:LAST_NAME Barbas ST:FIRST_NAME Coral ST:ADDRESS CEU Universities, Urbanización Montepríncipe ST:EMAIL cbarbas@ceu.es ST:PHONE +34 913724700 ST:SUBMIT_DATE 2022-06-09 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - HUPA_65 Label:AM | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=38; RAW_FILE_NAME=HUPA_65 SUBJECT_SAMPLE_FACTORS - HUPA_78 Label:AM | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=47; RAW_FILE_NAME=HUPA_78 SUBJECT_SAMPLE_FACTORS - HUPA_83 Label:AM | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=33; RAW_FILE_NAME=HUPA_83 SUBJECT_SAMPLE_FACTORS - HUPA_93 Label:AM | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=68; RAW_FILE_NAME=HUPA_93 SUBJECT_SAMPLE_FACTORS - HUPA_73 Label:AM | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=74; RAW_FILE_NAME=HUPA_73 SUBJECT_SAMPLE_FACTORS - HUPA_77_Bis Label:AM | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=65; RAW_FILE_NAME=HUPA_77_Bis SUBJECT_SAMPLE_FACTORS - HUPA_76 Label:AM | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:0 Age=90; RAW_FILE_NAME=HUPA_76 SUBJECT_SAMPLE_FACTORS - HT_12 Label:AM | Gender:0 | HTA:1 | Obesity:2 | Dyspnoea:0 Age=75; RAW_FILE_NAME=HT_12 SUBJECT_SAMPLE_FACTORS - HUPA_81 Label:AM | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=46; RAW_FILE_NAME=HUPA_81 SUBJECT_SAMPLE_FACTORS - HUPA_85 Label:AM | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=71; RAW_FILE_NAME=HUPA_85 SUBJECT_SAMPLE_FACTORS - HUPA_67 Label:AM | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:0 Age=39; RAW_FILE_NAME=HUPA_67 SUBJECT_SAMPLE_FACTORS - HUPA_97 Label:AM | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=72; RAW_FILE_NAME=HUPA_97 SUBJECT_SAMPLE_FACTORS - CS_11 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=67; RAW_FILE_NAME=CS_11 SUBJECT_SAMPLE_FACTORS - CS_42 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=77; RAW_FILE_NAME=CS_42 SUBJECT_SAMPLE_FACTORS - CS_66 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=69; RAW_FILE_NAME=CS_66 SUBJECT_SAMPLE_FACTORS - CS_78 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=63; RAW_FILE_NAME=CS_78 SUBJECT_SAMPLE_FACTORS - CS_79 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=65; RAW_FILE_NAME=CS_79 SUBJECT_SAMPLE_FACTORS - CS_84 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=67; RAW_FILE_NAME=CS_84 SUBJECT_SAMPLE_FACTORS - CS_86 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=67; RAW_FILE_NAME=CS_86 SUBJECT_SAMPLE_FACTORS - CS_87 Label:Healthy | Gender:0 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=63; RAW_FILE_NAME=CS_87 SUBJECT_SAMPLE_FACTORS - CS_15 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=72; RAW_FILE_NAME=CS_15 SUBJECT_SAMPLE_FACTORS - CS_41 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=67; RAW_FILE_NAME=CS_41 SUBJECT_SAMPLE_FACTORS - CS_48 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=65; RAW_FILE_NAME=CS_48 SUBJECT_SAMPLE_FACTORS - CS_5 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=69; RAW_FILE_NAME=CS_5 SUBJECT_SAMPLE_FACTORS - CS_54 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=44; RAW_FILE_NAME=CS_54 SUBJECT_SAMPLE_FACTORS - CS_55 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=52; RAW_FILE_NAME=CS_55 SUBJECT_SAMPLE_FACTORS - CS_77 Label:Healthy | Gender:1 | HTA:NA | Obesity:NA | Dyspnoea:NA Age=54; RAW_FILE_NAME=CS_77 SUBJECT_SAMPLE_FACTORS - CNMI_17 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=51; RAW_FILE_NAME=CNMI_17 SUBJECT_SAMPLE_FACTORS - CNMI_26 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=32; RAW_FILE_NAME=CNMI_26 SUBJECT_SAMPLE_FACTORS - CNMI_47 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=41; RAW_FILE_NAME=CNMI_47 SUBJECT_SAMPLE_FACTORS - CNMI_12 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=67; RAW_FILE_NAME=CNMI_12 SUBJECT_SAMPLE_FACTORS - CNMI_16 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=72; RAW_FILE_NAME=CNMI_16 SUBJECT_SAMPLE_FACTORS - CNMI_19 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=64; RAW_FILE_NAME=CNMI_19 SUBJECT_SAMPLE_FACTORS - CNMI_21 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=18; RAW_FILE_NAME=CNMI_21 SUBJECT_SAMPLE_FACTORS - CNMI_317 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=81; RAW_FILE_NAME=CNMI_317 SUBJECT_SAMPLE_FACTORS - CNMI_42 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=59; RAW_FILE_NAME=CNMI_42 SUBJECT_SAMPLE_FACTORS - CNMI_43 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=55; RAW_FILE_NAME=CNMI_43 SUBJECT_SAMPLE_FACTORS - CNMI_52 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=60; RAW_FILE_NAME=CNMI_52 SUBJECT_SAMPLE_FACTORS - CNMI_53 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=36; RAW_FILE_NAME=CNMI_53 SUBJECT_SAMPLE_FACTORS - CNMI_617 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=86; RAW_FILE_NAME=CNMI_617 SUBJECT_SAMPLE_FACTORS - CNMI_9 Label:Moderate | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=76; RAW_FILE_NAME=CNMI_9 SUBJECT_SAMPLE_FACTORS - CNMI_20_Bis Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=69; RAW_FILE_NAME=CNMI_20_Bis SUBJECT_SAMPLE_FACTORS - CNMI_48 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=75; RAW_FILE_NAME=CNMI_48 SUBJECT_SAMPLE_FACTORS - CNMI_611 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=82; RAW_FILE_NAME=CNMI_611 SUBJECT_SAMPLE_FACTORS - CNMI_76 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=84; RAW_FILE_NAME=CNMI_76 SUBJECT_SAMPLE_FACTORS - CNMI_24 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=84; RAW_FILE_NAME=CNMI_24 SUBJECT_SAMPLE_FACTORS - CNMI_25 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=63; RAW_FILE_NAME=CNMI_25 SUBJECT_SAMPLE_FACTORS - CNMI_44 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=86; RAW_FILE_NAME=CNMI_44 SUBJECT_SAMPLE_FACTORS - CNMI_46 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=70; RAW_FILE_NAME=CNMI_46 SUBJECT_SAMPLE_FACTORS - CNMI_55 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=72; RAW_FILE_NAME=CNMI_55 SUBJECT_SAMPLE_FACTORS - CNMI_56 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=93; RAW_FILE_NAME=CNMI_56 SUBJECT_SAMPLE_FACTORS - CNMI_61 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=69; RAW_FILE_NAME=CNMI_61 SUBJECT_SAMPLE_FACTORS - CNMI_75 Label:Moderate | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=59; RAW_FILE_NAME=CNMI_75 SUBJECT_SAMPLE_FACTORS - CNMI_32 Label:Moderate | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:0 Age=81; RAW_FILE_NAME=CNMI_32 SUBJECT_SAMPLE_FACTORS - CNMI_93_Bis Label:Moderate | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:0 Age=60; RAW_FILE_NAME=CNMI_93_Bis SUBJECT_SAMPLE_FACTORS - CNMI_68 Label:Moderate | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=60; RAW_FILE_NAME=CNMI_68 SUBJECT_SAMPLE_FACTORS - CNMI_86 Label:Moderate | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=58; RAW_FILE_NAME=CNMI_86 SUBJECT_SAMPLE_FACTORS - CNMI_18 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=57; RAW_FILE_NAME=CNMI_18 SUBJECT_SAMPLE_FACTORS - CNMI_2 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=61; RAW_FILE_NAME=CNMI_2 SUBJECT_SAMPLE_FACTORS - CNMI_27 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=48; RAW_FILE_NAME=CNMI_27 SUBJECT_SAMPLE_FACTORS - CNMI_30 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=42; RAW_FILE_NAME=CNMI_30 SUBJECT_SAMPLE_FACTORS - CNMI_36 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=63; RAW_FILE_NAME=CNMI_36 SUBJECT_SAMPLE_FACTORS - CNMI_41 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=60; RAW_FILE_NAME=CNMI_41 SUBJECT_SAMPLE_FACTORS - CNMI_51 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=55; RAW_FILE_NAME=CNMI_51 SUBJECT_SAMPLE_FACTORS - CNMI_63 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=59; RAW_FILE_NAME=CNMI_63 SUBJECT_SAMPLE_FACTORS - CNMI_64 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=38; RAW_FILE_NAME=CNMI_64 SUBJECT_SAMPLE_FACTORS - CNMI_1 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=51; RAW_FILE_NAME=CNMI_1 SUBJECT_SAMPLE_FACTORS - CNMI_14 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=64; RAW_FILE_NAME=CNMI_14 SUBJECT_SAMPLE_FACTORS - CNMI_22 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=CNMI_22 SUBJECT_SAMPLE_FACTORS - CNMI_34_Bis Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=CNMI_34_Bis SUBJECT_SAMPLE_FACTORS - CNMI_39 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=90; RAW_FILE_NAME=CNMI_39 SUBJECT_SAMPLE_FACTORS - CNMI_5 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=55; RAW_FILE_NAME=CNMI_5 SUBJECT_SAMPLE_FACTORS - CNMI_71 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=47; RAW_FILE_NAME=CNMI_71 SUBJECT_SAMPLE_FACTORS - CNMI_73 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=CNMI_73 SUBJECT_SAMPLE_FACTORS - CNMI_87 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=49; RAW_FILE_NAME=CNMI_87 SUBJECT_SAMPLE_FACTORS - CNMI_90 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=49; RAW_FILE_NAME=CNMI_90 SUBJECT_SAMPLE_FACTORS - CNMI_95 Label:Moderate | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=27; RAW_FILE_NAME=CNMI_95 SUBJECT_SAMPLE_FACTORS - CNMI_4 Label:Moderate | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:0 Age=49; RAW_FILE_NAME=CNMI_4 SUBJECT_SAMPLE_FACTORS - CNMI_246 Label:Moderate | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=76; RAW_FILE_NAME=CNMI_246 SUBJECT_SAMPLE_FACTORS - CNMI_28 Label:Moderate | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=51; RAW_FILE_NAME=CNMI_28 SUBJECT_SAMPLE_FACTORS - CNMI_29 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=57; RAW_FILE_NAME=CNMI_29 SUBJECT_SAMPLE_FACTORS - CNMI_31 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=82; RAW_FILE_NAME=CNMI_31 SUBJECT_SAMPLE_FACTORS - CNMI_59 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=75; RAW_FILE_NAME=CNMI_59 SUBJECT_SAMPLE_FACTORS - CNMI_89 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=56; RAW_FILE_NAME=CNMI_89 SUBJECT_SAMPLE_FACTORS - CNMI_13 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=CNMI_13 SUBJECT_SAMPLE_FACTORS - CNMI_45 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=57; RAW_FILE_NAME=CNMI_45 SUBJECT_SAMPLE_FACTORS - CNMI_58 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=77; RAW_FILE_NAME=CNMI_58 SUBJECT_SAMPLE_FACTORS - CNMI_6 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=74; RAW_FILE_NAME=CNMI_6 SUBJECT_SAMPLE_FACTORS - CNMI_91 Label:Moderate | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=56; RAW_FILE_NAME=CNMI_91 SUBJECT_SAMPLE_FACTORS - CNMI_72 Label:Moderate | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=65; RAW_FILE_NAME=CNMI_72 SUBJECT_SAMPLE_FACTORS - CNMI_78 Label:Moderate | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=57; RAW_FILE_NAME=CNMI_78 SUBJECT_SAMPLE_FACTORS - CNMI_0810 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=CNMI_810 SUBJECT_SAMPLE_FACTORS - HT_1 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=HT_1 SUBJECT_SAMPLE_FACTORS - HT_2 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=HT_2 SUBJECT_SAMPLE_FACTORS - HT_3 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=HT_3 SUBJECT_SAMPLE_FACTORS - HT_4 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=HT_4 SUBJECT_SAMPLE_FACTORS - HT_5 Label:NA | Gender:NA | HTA:NA | Obesity:NA | Dyspnoea:NA Age=NA; RAW_FILE_NAME=HT_5 SUBJECT_SAMPLE_FACTORS - HT_26 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=58; RAW_FILE_NAME=HT_26 SUBJECT_SAMPLE_FACTORS - CNMI_54 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=62; RAW_FILE_NAME=CNMI_54 SUBJECT_SAMPLE_FACTORS - CNMI_62 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=55; RAW_FILE_NAME=CNMI_62 SUBJECT_SAMPLE_FACTORS - HT_15 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=19; RAW_FILE_NAME=HT_15 SUBJECT_SAMPLE_FACTORS - HT_20 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=59; RAW_FILE_NAME=HT_20 SUBJECT_SAMPLE_FACTORS - HT_21 Label:Severe | Gender:0 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=69; RAW_FILE_NAME=HT_21 SUBJECT_SAMPLE_FACTORS - CNMI_97 Label:Severe | Gender:0 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=55; RAW_FILE_NAME=CNMI_97 SUBJECT_SAMPLE_FACTORS - CNMI_33 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=65; RAW_FILE_NAME=CNMI_33 SUBJECT_SAMPLE_FACTORS - CNMI_11 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=97; RAW_FILE_NAME=CNMI_11 SUBJECT_SAMPLE_FACTORS - CNMI_79 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=76; RAW_FILE_NAME=CNMI_79 SUBJECT_SAMPLE_FACTORS - CNMI_83 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=84; RAW_FILE_NAME=CNMI_83 SUBJECT_SAMPLE_FACTORS - CNMI_96 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=80; RAW_FILE_NAME=CNMI_96 SUBJECT_SAMPLE_FACTORS - HUPA_66 Label:Severe | Gender:0 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=59; RAW_FILE_NAME=HUPA_66 SUBJECT_SAMPLE_FACTORS - HT_24 Label:Severe | Gender:0 | HTA:1 | Obesity:1 | Dyspnoea:0 Age=47; RAW_FILE_NAME=HT_24 SUBJECT_SAMPLE_FACTORS - HT_13 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:0 Age=39; RAW_FILE_NAME=HT_13 SUBJECT_SAMPLE_FACTORS - CNMI_3 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=CNMI_3 SUBJECT_SAMPLE_FACTORS - CNMI_35 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=82; RAW_FILE_NAME=CNMI_35 SUBJECT_SAMPLE_FACTORS - CNMI_38 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=47; RAW_FILE_NAME=CNMI_38 SUBJECT_SAMPLE_FACTORS - CNMI_40 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=52; RAW_FILE_NAME=CNMI_40 SUBJECT_SAMPLE_FACTORS - CNMI_50 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=61; RAW_FILE_NAME=CNMI_50 SUBJECT_SAMPLE_FACTORS - CNMI_69 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=63; RAW_FILE_NAME=CNMI_69 SUBJECT_SAMPLE_FACTORS - CNMI_88 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=88; RAW_FILE_NAME=CNMI_88 SUBJECT_SAMPLE_FACTORS - HT_18_Bis Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=54; RAW_FILE_NAME=HT_18_Bis SUBJECT_SAMPLE_FACTORS - HT_22 Label:Severe | Gender:1 | HTA:0 | Obesity:0 | Dyspnoea:1 Age=60; RAW_FILE_NAME=HT_22 SUBJECT_SAMPLE_FACTORS - CNMI_15 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=49; RAW_FILE_NAME=CNMI_15 SUBJECT_SAMPLE_FACTORS - CNMI_60 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=58; RAW_FILE_NAME=CNMI_60 SUBJECT_SAMPLE_FACTORS - CNMI_85 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=53; RAW_FILE_NAME=CNMI_85 SUBJECT_SAMPLE_FACTORS - HT_14 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=20; RAW_FILE_NAME=HT_14 SUBJECT_SAMPLE_FACTORS - HT_19 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=31; RAW_FILE_NAME=HT_19 SUBJECT_SAMPLE_FACTORS - HT_23 Label:Severe | Gender:1 | HTA:0 | Obesity:1 | Dyspnoea:1 Age=42; RAW_FILE_NAME=HT_23 SUBJECT_SAMPLE_FACTORS - HT_11 Label:Severe | Gender:1 | HTA:0 | Obesity:2 | Dyspnoea:0 Age=87; RAW_FILE_NAME=HT_11 SUBJECT_SAMPLE_FACTORS - CNMI_7 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:0 Age=85; RAW_FILE_NAME=CNMI_7 SUBJECT_SAMPLE_FACTORS - CNMI_10 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=71; RAW_FILE_NAME=CNMI_10 SUBJECT_SAMPLE_FACTORS - CNMI_23 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=62; RAW_FILE_NAME=CNMI_23 SUBJECT_SAMPLE_FACTORS - CNMI_66 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=48; RAW_FILE_NAME=CNMI_66 SUBJECT_SAMPLE_FACTORS - CNMI_67 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=91; RAW_FILE_NAME=CNMI_67 SUBJECT_SAMPLE_FACTORS - CNMI_70 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=77; RAW_FILE_NAME=CNMI_70 SUBJECT_SAMPLE_FACTORS - CNMI_74 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=61; RAW_FILE_NAME=CNMI_74 SUBJECT_SAMPLE_FACTORS - CNMI_8 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=89; RAW_FILE_NAME=CNMI_8 SUBJECT_SAMPLE_FACTORS - CNMI_81 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=87; RAW_FILE_NAME=CNMI_81 SUBJECT_SAMPLE_FACTORS - CNMI_92 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=92; RAW_FILE_NAME=CNMI_92 SUBJECT_SAMPLE_FACTORS - HT_17 Label:Severe | Gender:1 | HTA:1 | Obesity:0 | Dyspnoea:1 Age=69; RAW_FILE_NAME=HT_17 SUBJECT_SAMPLE_FACTORS - CNMI_37 Label:Severe | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=50; RAW_FILE_NAME=CNMI_37 SUBJECT_SAMPLE_FACTORS - CNMI_49 Label:Severe | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=79; RAW_FILE_NAME=CNMI_49 SUBJECT_SAMPLE_FACTORS - CNMI_82 Label:Severe | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=57; RAW_FILE_NAME=CNMI_82 SUBJECT_SAMPLE_FACTORS - CNMI_94 Label:Severe | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=66; RAW_FILE_NAME=CNMI_94 SUBJECT_SAMPLE_FACTORS - HT_16 Label:Severe | Gender:1 | HTA:1 | Obesity:1 | Dyspnoea:1 Age=71; RAW_FILE_NAME=HT_16 #COLLECTION CO:COLLECTION_SUMMARY Samples were collected at hospital admission or within the first days after CO:COLLECTION_SUMMARY hospitalization (median = 2 days) and before treatment with immunotherapy CO:COLLECTION_SUMMARY against IL-6 (e.g., tocilizumab), interferon beta, corticoids, or ribavirin, CO:COLLECTION_SUMMARY among others. Plasma samples were obtained after centrifugation blood in EDTA CO:COLLECTION_SUMMARY tubes and stored at -80 °C. CO:SAMPLE_TYPE Blood (plasma) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Plasma samples were inactivated with cold (-20°C) MeOH : EtOH (1:1, v/v) and TR:TREATMENT_SUMMARY vortex-mixed for 1 min, incubated on ice for 5 min, and centrifuged for 20 min TR:TREATMENT_SUMMARY at 16000 xg at 4°C. The resulting supernatant was stored at -80°C until TR:TREATMENT_SUMMARY analysis. Due to the broad differences in physical-chemical properties of TR:TREATMENT_SUMMARY metabolites, we used GC-MS (focused on small molecules that can be made volatile TR:TREATMENT_SUMMARY by derivatization) and CE-MS (focused on polar and ionic compounds) in order to TR:TREATMENT_SUMMARY increase the metabolite coverage. Sample preparation for GC-MS and CE-MS was TR:TREATMENT_SUMMARY detailed in the uploaded file in sample preparation part. TR:TREATMENT_PROTOCOL_FILENAME Sample_treatment.pdf #SAMPLEPREP SP:SAMPLEPREP_SUMMARY For GC-MS analysis samples, 200 µL of frozen plasma supernatant was thawed at SP:SAMPLEPREP_SUMMARY room temperature and 30 µL of 80 mg/L deuterated palmitic acid in MeOH was SP:SAMPLEPREP_SUMMARY added as internal standard (IS). After samples were evaporated to dryness a SP:SAMPLEPREP_SUMMARY two-step derivatization process was done. For CE-MS analysis, 200 µL of frozen SP:SAMPLEPREP_SUMMARY supernatant was thawed until room temperature and it was evaporated to dryness SP:SAMPLEPREP_SUMMARY using a SpeedVac Concentrator System (Thermo Fisher Scientific, MA). Afterwards, SP:SAMPLEPREP_SUMMARY 100 µL of 0.2 mM methionine sulfone (MetS) as internal standard (IS) in 0.1 M SP:SAMPLEPREP_SUMMARY formic acid solution was added. Samples were vortex mixed, filtered and SP:SAMPLEPREP_SUMMARY subsequently centrifuged. More detailed information is included in the attached SP:SAMPLEPREP_SUMMARY pdf. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Metabolite separation was performed in a fused silica capillary (100 cm; inner CH:CHROMATOGRAPHY_SUMMARY diameter, 50 µm, Agilent Technologies). Before each analysis, background CH:CHROMATOGRAPHY_SUMMARY electrolyte (BFE) (0.8 M formic acid solution in 10 % MeOH; v/v) was flushed for CH:CHROMATOGRAPHY_SUMMARY 5 min (950 mbar). Samples injections were performed over 50 s at 50 mbar and BGE CH:CHROMATOGRAPHY_SUMMARY was injected after each injection for 10 s at 100 mbar to improve CH:CHROMATOGRAPHY_SUMMARY reproducibility. The separation was carried out with an internal pressure of 25 CH:CHROMATOGRAPHY_SUMMARY mbar and 30 kV voltage with a total analytical run time of 35 min. CH:METHODS_FILENAME Sample_Analysis.pdf CH:INSTRUMENT_NAME Agilent 7100 CE CH:COLUMN_NAME silica capillary (100 cm; inner diameter, 50 µm, Agilent Technologies) CH:CHROMATOGRAPHY_TYPE Other #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE Sample_Analysis.pdf #MS MS:INSTRUMENT_NAME Agilent CE-TOFMS MS:INSTRUMENT_TYPE TOF MS:MS_TYPE ESI MS:MS_COMMENTS Data were acquired in positive ionization polarity with a full scan range from MS:MS_COMMENTS 70 to 1000 m/z at a rate of 1.36 scan/s. The rest of MS conditions were: MS:MS_COMMENTS fragmentor set to 125 V, skimmer to 65 V, OCT RF Vpp to 750 V, drying gas MS:MS_COMMENTS temperature to 200 °C, flow rate to 10 L/min, nebulizer to 10 psig, and MS:MS_COMMENTS capillary voltage to 3500 V. The sheath liquid used for detection contained two MS:MS_COMMENTS reference masses (5 µL of purine with m/z 121.0509 and 5 µL of HP-0921 with MS:MS_COMMENTS m/z 922.0098) in MeOH/water (1/1; v/v) with 1 mM formic acid and the flow rate MS:MS_COMMENTS was set to 0.6 mL/min (split 1:100). The data acquisition was made using the MS:MS_COMMENTS Agilent MassHunter Workstation (Agilent Technologies). MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST002194_AN003591_Results.txt UNITS:Peak Area Has RT:Yes RT units:Minutes #END