#METABOLOMICS WORKBENCH Andre_Gollowitzer_20221012_041732 DATATRACK_ID:3503 STUDY_ID:ST002325 ANALYSIS_ID:AN003794 PROJECT_ID:PR001490 VERSION 1 CREATED_ON October 26, 2022, 6:21 pm #PROJECT PR:PROJECT_TITLE Reducing the metabolic burden of rRNA synthesis promotes healthy longevity PR:PROJECT_SUMMARY Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol PR:PROJECT_SUMMARY I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was PR:PROJECT_SUMMARY previously linked to longevity, the underlying mechanisms were not studied PR:PROJECT_SUMMARY beyond effects on protein translation and downstream proteostasis. Here we used PR:PROJECT_SUMMARY multi-omics and functional tests to show that curtailment of Pol I activity PR:PROJECT_SUMMARY preserves mitochondrial function and lowers ATP expenditure, thereby promoting PR:PROJECT_SUMMARY longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved PR:PROJECT_SUMMARY longevity and energy homeostasis in Drosophila melanogaster and human cells, PR:PROJECT_SUMMARY respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth PR:PROJECT_SUMMARY and neuromuscular performance of young nematodes at the cost of accelerated PR:PROJECT_SUMMARY metabolic decline, mitochondrial stress and premature aging. Moreover, PR:PROJECT_SUMMARY restriction of Pol I activity extended lifespan more potently than translational PR:PROJECT_SUMMARY repression, and retained its geroprotective effects when initiated late in life, PR:PROJECT_SUMMARY showcasing moderation of Pol I activity as an effective longevity treatment not PR:PROJECT_SUMMARY limited by aging. PR:INSTITUTE University of Innsbruck PR:DEPARTMENT Michael Popp Institute PR:LAST_NAME Koeberle PR:FIRST_NAME Andreas PR:ADDRESS Mitterweg 24, Innsbruck, Tyrol, 6020, Austria PR:EMAIL andreas.koeberle@uibk.ac.at PR:PHONE +43 512 507 57903 PR:FUNDING_SOURCE Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of PR:FUNDING_SOURCE Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), PR:FUNDING_SOURCE Phospholipid Research Center (AKO-2019-070/2-1) PR:CONTRIBUTORS Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, PR:CONTRIBUTORS Maria Ermolaeva #STUDY ST:STUDY_TITLE Determination of changes in free fatty acid levels in C. elegans depleted of ST:STUDY_TITLE tif-IA and ncl-1 ST:STUDY_SUMMARY Analysis of free fatty acids in young (d2), middle age (d6) and old age (d12) C. ST:STUDY_SUMMARY elegans with tif-IA or ncl-1 knockdown. ST:INSTITUTE University of Innsbruck ST:DEPARTMENT Michael Popp Institute ST:LAST_NAME Koeberle ST:FIRST_NAME Andreas ST:ADDRESS Mitterweg 24, Innsbruck, Tyrol, 6020, Austria ST:EMAIL andreas.koeberle@uibk.ac.at ST:PHONE +43 512 507 57903 #SUBJECT SU:SUBJECT_TYPE Invertebrate SU:SUBJECT_SPECIES Caenorhabditis elegans SU:TAXONOMY_ID 6239 SU:AGE_OR_AGE_RANGE Young, middle aged and old #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_1 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_2 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_3 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_4 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_5 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_6 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_7 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_8 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_9 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_10 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_11 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_12 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_13 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_14 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_15 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_16 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_17 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_18 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_19 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_20 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_21 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_22 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_23 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_24 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_25 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_26 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_27 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_28 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_29 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_30 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_31 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_32 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_33 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_34 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_35 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_36 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_37 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_38 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_39 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_40 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_41 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_42 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_43 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_44 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff SUBJECT_SAMPLE_FACTORS - 200301_Ermoleva_FFA_45 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff #COLLECTION CO:COLLECTION_SUMMARY Worms were washed and pelleted, M9 media was removed with ~100µl remaining in CO:COLLECTION_SUMMARY the sample, and the sample was snap frozen. CO:SAMPLE_TYPE Worms #TREATMENT TR:TREATMENT_SUMMARY Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations TR:TREATMENT_SUMMARY with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) TR:TREATMENT_SUMMARY producing the corresponding dsRNA. Worms were cultured under standard conditions TR:TREATMENT_SUMMARY as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 TR:TREATMENT_SUMMARY animals were grown on RNAi until days 2, 6 or 12 of adulthood. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Free fatty acids were extracted from C. elegans by successive addition of PBS pH SP:SAMPLEPREP_SUMMARY 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. SP:SAMPLEPREP_SUMMARY Evaporation of the organic layer yielded a lipid film that was dissolved in SP:SAMPLEPREP_SUMMARY methanol and subjected to UPLC-MS/MS. SP:EXTRACT_STORAGE -20℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Chromatographic separation of free fatty acids was carried out on an Acquity BEH CH:CHROMATOGRAPHY_SUMMARY C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity H-Class CH:COLUMN_NAME Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Multiple ion monitoring with pre-optimized settings and subsequent automated MS:MS_COMMENTS integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS relative units MS_METABOLITE_DATA_START Samples 200301_Ermoleva_FFA_1 200301_Ermoleva_FFA_2 200301_Ermoleva_FFA_3 200301_Ermoleva_FFA_4 200301_Ermoleva_FFA_5 200301_Ermoleva_FFA_6 200301_Ermoleva_FFA_7 200301_Ermoleva_FFA_8 200301_Ermoleva_FFA_9 200301_Ermoleva_FFA_10 200301_Ermoleva_FFA_11 200301_Ermoleva_FFA_12 200301_Ermoleva_FFA_13 200301_Ermoleva_FFA_14 200301_Ermoleva_FFA_15 200301_Ermoleva_FFA_16 200301_Ermoleva_FFA_17 200301_Ermoleva_FFA_18 200301_Ermoleva_FFA_19 200301_Ermoleva_FFA_20 200301_Ermoleva_FFA_21 200301_Ermoleva_FFA_22 200301_Ermoleva_FFA_23 200301_Ermoleva_FFA_24 200301_Ermoleva_FFA_25 200301_Ermoleva_FFA_26 200301_Ermoleva_FFA_27 200301_Ermoleva_FFA_28 200301_Ermoleva_FFA_29 200301_Ermoleva_FFA_30 200301_Ermoleva_FFA_31 200301_Ermoleva_FFA_32 200301_Ermoleva_FFA_33 200301_Ermoleva_FFA_34 200301_Ermoleva_FFA_35 200301_Ermoleva_FFA_36 200301_Ermoleva_FFA_37 200301_Ermoleva_FFA_38 200301_Ermoleva_FFA_39 200301_Ermoleva_FFA_40 200301_Ermoleva_FFA_41 200301_Ermoleva_FFA_42 200301_Ermoleva_FFA_43 200301_Ermoleva_FFA_44 200301_Ermoleva_FFA_45 Factors Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Myristic acid 45.541074827939700 39.436878066675800 19.695682397466600 35.956095714706300 14.848095849379500 29.301745635910200 16.766509517564100 14.562071050104900 15.737587381251100 20.906563742045200 31.397991222440000 28.188746917731500 41.658764776534500 36.906906020254500 16.031009235775500 21.683793721127600 18.309214464279400 29.725609756097600 46.428980855210400 41.846906150084600 10.825267447784000 55.558387277639000 5.652911249293390 24.171981073853100 36.379986919555300 37.929222144358800 35.610320213741700 46.681550639334300 22.157434402332400 36.130068245684500 44.181326116373500 14.093959731543600 22.836538461538500 22.903094462540700 2.493765586034910 37.847688086989600 54.311435161074300 30.355021776428700 14.966699094514700 29.537734455767200 35.410216718266300 36.699507389162600 35.990261458664100 34.735260983852800 34.868754631615900 Hexadecenoic acid 11.055791477522300 9.148438574769570 18.807445740325900 11.060179149669900 8.600770218228500 16.608478802992500 6.912197369820280 8.054478859215530 3.082989783115250 6.892273761113790 2.151022603084290 12.216991705895500 13.907326632530500 13.345537216924000 17.586256400440300 2.231230948116020 0.000000000000000 12.576219512195100 12.438381290840300 11.190520970472100 8.182628629648500 8.767011570416430 15.381869030972000 13.114585476239500 11.118378024852800 5.737561317449190 9.730031502970850 7.458899939111020 12.303206997084500 7.455410907839650 10.148849797023000 0.000000000000000 13.942307692307700 4.275244299674270 9.289276807980050 7.149007749764700 12.499070009671900 13.461792266068400 6.735014592531620 6.719834588687050 8.191434468524250 26.108374384236500 3.387318725521330 5.867442733758920 4.524357424236510 Octadecenoic acid 14.643432420559400 19.489154858531700 20.468062099328000 14.718869590815400 25.531307944658400 35.162094763092300 15.951224699585300 15.468866683261600 21.867718229073300 21.595791118156500 23.244921678491500 26.619592019726500 19.912763132941400 15.648528152587900 37.086663157391000 2.828320920147070 12.323509735572700 16.768292682926800 17.195918835263100 0.000000000000000 24.834437086092700 0.000000000000000 54.743982624735900 20.057601316601500 0.000000000000000 0.000000000000000 14.355784184711100 18.012989648873600 17.201166180758000 18.925273842977600 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 20.425736427899200 20.459787218212900 17.817077999208100 14.966699094514700 23.113277211637900 27.089783281733700 11.494252873563200 8.997565364666030 6.102140443109270 30.422403369866200 Octadecadienoic acid 0.000000000000000 0.068785252441877 0.000000000000000 0.000000000000000 5.348737697903300 1.271820448877810 2.552195951933640 7.947797020020620 1.756587202007530 0.000000000000000 4.128575641403720 0.812598072181125 0.000000000000000 1.033393368567130 1.004928937168010 0.754218912039219 0.070420055631844 0.647865853658537 0.000000000000000 0.000000000000000 2.738155883851250 0.000000000000000 2.975216446996520 0.000000000000000 0.000000000000000 0.000000000000000 2.631893767197030 0.000000000000000 2.215743440233240 0.200722601364914 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 7.269159140517050 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.000000000000000 0.093879083740143 0.000000000000000 Eicosatetraenoic acid 2.086689119929710 3.402577154124820 6.503437089673280 5.000210269565580 6.931964056482670 3.802992518703240 5.161107369465810 6.920095302443010 10.055565513532900 3.668987065499580 3.464187208354290 4.898004931629680 3.723370630254760 5.311641914435030 6.350193807723600 7.891015367210330 5.531495369881340 6.402439024390240 2.097902097902100 4.527929283430510 9.389327559857360 5.092002650491870 3.674392312040700 9.535075087430570 4.120340091563110 5.089348283111420 6.208876659887550 1.585650497260000 4.373177842565600 6.397316052073180 7.171853856562920 18.389261744966400 11.237980769230800 5.690146579804560 10.642144638404000 5.020325610268940 1.421025221337700 1.940081826580440 10.416822569782200 7.413971348397580 5.572755417956660 6.568144499178980 3.673123742987190 5.510702215546380 4.169429384921410 Eicosapentaenoic acid 26.673012154048900 28.454166093456200 34.525372673206100 33.264645275242900 38.739124233347600 13.852867830423900 52.656765091630900 47.046691084954300 47.499551891019900 46.936384313184900 35.613301646226200 27.264066352835700 20.797774827738800 27.753993327231400 21.940948461501700 64.611420131359800 63.765360374634700 33.879573170731700 21.838816920784100 42.434643596012800 44.030183392766200 30.582598501452700 17.571628335961400 33.120757045875300 48.381294964028800 51.243868255080600 31.463093671491800 26.260909275421100 41.749271137026200 30.891208350060200 38.497970230040600 67.516778523489900 51.983173076923100 67.131514657980500 77.574812967581100 22.288082984560500 11.308682389703100 36.426026131714400 52.914764648656700 33.215182395510300 23.735810113519100 19.129720853858800 47.951730708161300 47.690574539992500 26.015055189360000 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Standardized name Alternative name Formula Exact mass Super class Main class Sub class Myristic acid Myristic acid FA 14:0 C14H28O2 228.2089 Fatty Acyls Fatty acids Saturated FA Hexadecenoic acid Hexadecenoic acid FA 16:1 C16H30O2 254.2246 Fatty Acyls Fatty acids Unsaturated FA Octadecenoic acid Octadecenoic acid FA 18:1 C18H34O2 282.2559 Fatty Acyls Fatty acids Unsaturated FA Octadecadienoic acid Octadecadienoic acid FA 18:2 C18H32O2 280.2402 Fatty Acyls Fatty acids Unsaturated FA Eicosatetraenoic acid Arachidonic acid FA 20:4 C20H32O2 304.2402 Fatty Acyls Fatty acids Unsaturated FA Eicosapentaenoic acid Eicosapentaenoic acid FA 20:5 C20H30O2 302.2246 Fatty Acyls Fatty acids Unsaturated FA METABOLITES_END #END