#METABOLOMICS WORKBENCH annakisiala_20221017_123232 DATATRACK_ID:3515 STUDY_ID:ST002330 ANALYSIS_ID:AN003801 PROJECT_ID:PR001494 VERSION 1 CREATED_ON November 2, 2022, 8:12 am #PROJECT PR:PROJECT_TITLE Early-stage responses to Plasmodiophora brassicae at the metabolome levels in PR:PROJECT_TITLE clubroot resistant and susceptible oilseed Brassica napus PR:PROJECT_TYPE MS quantitative analysis PR:PROJECT_SUMMARY Clubroot, a devastating soil-borne root disease, in Brassicaceae is caused by PR:PROJECT_SUMMARY Plasmodiophora brassicae Woronin, an obligate biotrophic protist. Plant growth PR:PROJECT_SUMMARY and development, as well as seed yield of Brassica crops, are severely affected PR:PROJECT_SUMMARY due to this disease. Several reports described the molecular responses of B. PR:PROJECT_SUMMARY napus to P. brassicae; however, information on the early stages of pathogenesis PR:PROJECT_SUMMARY is limited. In this study, we have used metabolomics approach to characterize P. PR:PROJECT_SUMMARY brassicae pathogenesis at 1-, 4-, and 7- days post-inoculation (DPI) in clubroot PR:PROJECT_SUMMARY resistant (CR) and susceptible (CS) doubled-haploid (DH) lines. Several PR:PROJECT_SUMMARY metabolites related to organic acids (e.g., citrate, pyruvate), amino acids PR:PROJECT_SUMMARY (e.g., proline, aspartate), sugars, and mannitol, were differentially PR:PROJECT_SUMMARY accumulated in roots in response to pathogen infection when the CR and CS PR:PROJECT_SUMMARY genotypes were compared. Our results suggest important roles for these PR:PROJECT_SUMMARY metabolites in mediating resistance to clubroot disease. To our knowledge, this PR:PROJECT_SUMMARY is the first report of an integrated metabolome analysis aimed at characterizing PR:PROJECT_SUMMARY the molecular basis of resistance to clubroot in canola. PR:INSTITUTE Trent University PR:DEPARTMENT Biology PR:LABORATORY Emery Lab PR:LAST_NAME Kisiala PR:FIRST_NAME Anna PR:ADDRESS 1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada PR:EMAIL annakisiala@trentu.ca PR:PHONE 7057481011 PR:FUNDING_SOURCE Alberta Agriculture and Forestry PR:CONTRIBUTORS Dinesh Adhikary, Anna Kisiala, Ananya Sarkar, Urmila Basu, Habibur Rahman, Neil PR:CONTRIBUTORS Emery, Nat N. V. Kav #STUDY ST:STUDY_TITLE Early-stage responses to Plasmodiophora brassicae at the metabolome levels in ST:STUDY_TITLE clubroot resistant and susceptible oilseed Brassica napus ST:STUDY_TYPE Timecourse experiment ST:STUDY_SUMMARY A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled ST:STUDY_SUMMARY in each biological replicate) and CS (5 individuals pooled in each biological ST:STUDY_SUMMARY replicate)], two treatments (inoculated and uninoculated) and three biological ST:STUDY_SUMMARY replicates generated from three independent experiments and collected at 1-, 4-, ST:STUDY_SUMMARY and 7–DPI were used to extract primary and secondary metabolites and analyse ST:STUDY_SUMMARY the differences among the treatments. ST:INSTITUTE Trent University ST:DEPARTMENT Biology ST:LABORATORY Emery Lab ST:LAST_NAME Kisiala ST:FIRST_NAME Anna ST:ADDRESS 1600 West Bank Drive, Trent University ST:EMAIL annakisiala@trentu.ca ST:PHONE 7057481011 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Brassica napus SU:TAXONOMY_ID 3708 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - C18-RN1-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN1-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN1-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN1-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN1-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN1-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN1-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN1-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN2-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN2-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN2-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN2-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN2-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN2-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN2-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN2-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN3-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN3-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN3-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN3-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN3-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN3-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN3-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN3-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN4-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN4-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN4-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN4-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN4-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN4-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN4-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN4-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN5-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN5-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN5-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN5-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN5-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN5-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN5-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN5-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN6-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN6-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN6-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN6-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN6-pos Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN6-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN6-neg Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN6-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN7-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN7-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN7-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN7-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN7-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN7-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN7-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN7-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN8-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN8-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN8-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN8-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN8-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN8-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN8-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN8-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN9-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN9-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN9-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN9-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN9-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN9-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN9-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN9-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN10-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN10-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN10-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN10-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN10-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN10-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN10-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN10-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN11-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN11-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN11-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN11-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN11-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN11-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN11-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN11-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN12-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN12-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN12-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN12-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN12-pos Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN12-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN12-neg Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN12-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN13-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN13-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN13-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN13-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN13-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN13-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN13-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN13-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN14-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN14-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN14-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN14-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN14-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN14-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN14-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN14-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN15-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN15-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN15-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN15-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN15-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN15-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN15-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN15-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN16-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN16-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN16-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN16-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN16-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN16-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN16-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN16-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN17-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN17-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN17-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN17-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN17-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN17-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN17-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN17-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN18-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN18-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN18-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN18-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN18-pos Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN18-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN18-neg Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN18-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN19-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN19-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN19-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN19-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN19-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN19-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN19-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN19-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN20-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN20-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN20-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN20-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN20-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN20-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN20-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN20-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN21-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN21-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN21-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN21-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN21-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN21-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN21-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN21-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN22-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN22-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN22-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN22-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN22-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN22-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN22-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN22-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN23-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN23-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN23-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN23-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN23-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN23-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN23-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN23-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN24-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN24-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN24-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN24-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN24-pos Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN24-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN24-neg Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN24-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN25-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN25-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN25-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN25-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN25-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN25-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN25-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN25-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN26-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN26-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN26-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN26-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN26-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN26-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN26-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN26-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN27-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN27-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN27-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=C18-RN27-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN27-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN27-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN27-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control RAW_FILE_NAME=HILIC-RN27-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN28-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN28-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN28-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN28-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN28-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN28-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN28-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN28-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN29-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN29-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN29-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN29-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN29-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN29-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN29-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN29-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN30-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN30-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN30-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN30-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN30-pos Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN30-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN30-neg Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN30-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN31-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN31-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN31-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN31-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN31-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN31-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN31-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN31-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN32-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN32-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN32-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN32-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN32-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN32-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN32-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN32-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN33-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN33-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN33-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=C18-RN33-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN33-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN33-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN33-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control RAW_FILE_NAME=HILIC-RN33-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN34-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN34-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN34-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN34-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN34-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN34-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN34-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN34-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN35-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN35-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN35-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN35-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN35-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN35-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN35-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN35-neg.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN36-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN36-pos.mzXML SUBJECT_SAMPLE_FACTORS - C18-RN36-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=C18-RN36-neg.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN36-pos Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN36-pos.mzXML SUBJECT_SAMPLE_FACTORS - HILIC-RN36-neg Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae RAW_FILE_NAME=HILIC-RN36-neg.mzXML #COLLECTION CO:COLLECTION_SUMMARY A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled CO:COLLECTION_SUMMARY in each biological replicate) and CS (5 individuals pooled in each biological CO:COLLECTION_SUMMARY replicate)], two treatments (inoculated and uninoculated) and three biological CO:COLLECTION_SUMMARY replicates generated from three independent experiments and collected at 1-, 4-, CO:COLLECTION_SUMMARY and 7–DPI were used. Immediately after collection, all root samples were CO:COLLECTION_SUMMARY flash-frozen in liquid nitrogen and stored at -80 °C. CO:COLLECTION_PROTOCOL_FILENAME Collection_protocol-canola CO:SAMPLE_TYPE Plant CO:COLLECTION_LOCATION Alberta, Canada CO:STORAGE_CONDITIONS -80℃ CO:COLLECTION_VIALS 2 mL round-bottom Eppendorf tubes CO:STORAGE_VIALS 2 mL round-bottom Eppendorf tubes #TREATMENT TR:TREATMENT_SUMMARY A single spore-derived isolate of P. brassicae pathotype 3H (P3H) was used in TR:TREATMENT_SUMMARY this study. To assess the disease progression post-inoculation, histology, and TR:TREATMENT_SUMMARY scanning electron microscopy (SEM) was conducted on P. brassicae inoculated and TR:TREATMENT_SUMMARY uninoculated root tissues. Samples were collected by Adhikary as follows: TR:TREATMENT_SUMMARY seedlings were randomly selected, gently uprooted from the soil, the roots were TR:TREATMENT_SUMMARY washed thoroughly and collected in triplicates at after 24 hours of inoculation TR:TREATMENT_SUMMARY [hereinafter indicated as 1-day post-inoculation (DPI)], 4-, and 7-DPI. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Approximately 100 mg of fresh weight of root tissue samples (n=3) were aliquoted SP:SAMPLEPREP_SUMMARY from finely homogenized frozen root tissues, placed into 2 mL safe-lock SP:SAMPLEPREP_SUMMARY centrifuge tubes with two zirconium oxide beads, flash frozen in liquid nitrogen SP:SAMPLEPREP_SUMMARY and stored at − 80 °C until further processing. To accommodate for different SP:SAMPLEPREP_SUMMARY extraction procedures, two sample sets were prepared from the same bulk to SP:SAMPLEPREP_SUMMARY independently analyze the content of primary metabolites (free amino acids, SP:SAMPLEPREP_SUMMARY sugars and sugar phosphates, organic acids), and secondary metabolites SP:SAMPLEPREP_SUMMARY (glucosinolates) using High Performance Liquid Chromatography – SP:SAMPLEPREP_SUMMARY High-Resolution Accurate Mass – Full Scan Mass Spectrometry (HPLC-(HRAM)-MS). SP:SAMPLEPREP_SUMMARY Samples for extraction of primary and secondary metabolites were spiked with SP:SAMPLEPREP_SUMMARY 10ng of two labeled aromatic CKs (13C5-oT and 2H7-BAR) and extracted with SP:SAMPLEPREP_SUMMARY ice-cold methanol (methanol-water [8:2, v/v]) following procedures of Chen et al SP:SAMPLEPREP_SUMMARY 3 with modifications. Each filtered extract was split in half and each 500 µL SP:SAMPLEPREP_SUMMARY transferred to a new 2 mL tube. Divided extracts were evaporated to dryness at SP:SAMPLEPREP_SUMMARY ambient temperature in a speed vac concentrator. Sample residues designed for SP:SAMPLEPREP_SUMMARY the analysis of primary metabolites were redissolved in 500 µl of 90% SP:SAMPLEPREP_SUMMARY acetonitrile (acetonitrile: water, v/v) and samples for the analysis of SP:SAMPLEPREP_SUMMARY glucosinolates were redissolved in 500 µL of 5% acetonitrile with 0.1% formic SP:SAMPLEPREP_SUMMARY acid (acetonitrile: formic acid: water, v/v/v). Additionally, sample mixtures SP:SAMPLEPREP_SUMMARY composed of 10uL of each sample extract were prepared separately for primary and SP:SAMPLEPREP_SUMMARY secondary metabolite analysis and used to generate MS/MS data for compound SP:SAMPLEPREP_SUMMARY identification. All samples were filtered using 0.2 µm PVDF spin filter with 2 SP:SAMPLEPREP_SUMMARY mL receiver tubes (InnoSep Spin, Canadian Life Sciences, Peterborough, Canada) SP:SAMPLEPREP_SUMMARY and transferred to insert-equipped 2 mL HPLC vials. A volume of 25 μL of each SP:SAMPLEPREP_SUMMARY sample was injected into a Dionex UltiMate 3000 HPLC coupled to a QExactive SP:SAMPLEPREP_SUMMARY Orbitrap mass spectrometer. SP:SAMPLEPREP_PROTOCOL_FILENAME Protocol_methods-canola SP:EXTRACTION_METHOD 80% MeOH SP:EXTRACT_CLEANUP 0.2 µm PVDF spin filter with 2 mL receiver tubes SP:EXTRACT_STORAGE -20℃ SP:SAMPLE_RESUSPENSION Primary metabolites - 90% ACN, glucosinolates - 5% ACN SP:SAMPLE_SPIKING two labeled aromatic CKs (13C5-oT and 2H7-BAR) #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Primary metabolites were resolved with an InfinityLab Poroshell 120 HILIC-Z CH:CHROMATOGRAPHY_SUMMARY column (2.1 × 100 mm, 2.7 µm; Agilent, Santa Clara, CA, US), using a flow rate CH:CHROMATOGRAPHY_SUMMARY of 0.2 mL min-1 with a mobile phase of 10 mM ammonium bicarbonate in water (A) CH:CHROMATOGRAPHY_SUMMARY and 10 mM ammonium bicarbonate in 95% acetonitrile (B). The following gradient CH:CHROMATOGRAPHY_SUMMARY was used to elute the analytes: mobile phase 100% B decreased to 90% over 2.5 CH:CHROMATOGRAPHY_SUMMARY min and to 50% over the next 5 min and returned to 100% over 0.5 min for 12 min CH:CHROMATOGRAPHY_SUMMARY of column re-equilibration. CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 RS CH:COLUMN_NAME Agilent, InfinityLab Poroshell 120 HILIC-Z (2.1 × 100 mm, 2.7 µm) CH:FLOW_RATE 0.2 ml/min CH:COLUMN_TEMPERATURE RT CH:SOLVENT_A 10 mM ammonium bicarbonate in water CH:SOLVENT_B 10 mM ammonium bicarbonate in 95% acetonitrile CH:INJECTION_TEMPERATURE 4 #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME WQC AN:OPERATOR_NAME Anna Kisiala #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS The Orbitrap QExactive was operated with a heated electrospray ionization (HESI) MS:MS_COMMENTS probe in positive mode. Temperatures of the HESI-II auxiliary gas heater and MS:MS_COMMENTS capillary were 450 and 300 °C, respectively, and the spray voltage was 3.9 kV. MS:MS_COMMENTS Sheath, auxiliary, and sweep gases were operated at 30, 8, and 0 (arbitrary MS:MS_COMMENTS units), respectively, and the S-lens RF level was 60. Each sample was analyzed MS:MS_COMMENTS using a mass range of m/z 70−900, and data were acquired at 70,000 resolution, MS:MS_COMMENTS automatic gain control (AGC) target of 1e6, and maximum injection time (IT) of MS:MS_COMMENTS 100 ms. Additionally, the top 10 data-dependent acquisition experiments were MS:MS_COMMENTS performed for the mixed sample from each group (HILIC and C18) to obtain MS:MS_COMMENTS compound MS/MS spectra. Processing of all full scan and ddMS2 data was conducted MS:MS_COMMENTS using the Xcalibur 4.1 software. Metabolites were identified by accurate mass, MS:MS_COMMENTS comparison of retention times to authentic standards or by accurate mass and MS:MS_COMMENTS comparison of fragmentation patterns to MS/MS databases (METLIN, PubChem). MS:MS_COMMENTS Metabolite relative concentration was normalized based on the average recoveries MS:MS_COMMENTS of 13C5-oT and 2H7-BAR as the internal standards #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS normalized relative level MS_METABOLITE_DATA_START Samples HILIC-RN1-pos HILIC-RN2-pos HILIC-RN3-pos HILIC-RN4-pos HILIC-RN5-pos HILIC-RN6-pos HILIC-RN7-pos HILIC-RN8-pos HILIC-RN9-pos HILIC-RN10-pos HILIC-RN11-pos HILIC-RN12-pos HILIC-RN13-pos HILIC-RN14-pos HILIC-RN15-pos HILIC-RN16-pos HILIC-RN17-pos HILIC-RN18-pos HILIC-RN19-pos HILIC-RN20-pos HILIC-RN21-pos HILIC-RN22-pos HILIC-RN23-pos HILIC-RN24-pos HILIC-RN25-pos HILIC-RN26-pos HILIC-RN27-pos HILIC-RN28-pos HILIC-RN29-pos HILIC-RN30-pos HILIC-RN31-pos HILIC-RN32-pos HILIC-RN33-pos HILIC-RN34-pos HILIC-RN35-pos HILIC-RN36-pos Factors Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae Alanine 0.802 0.470 0.463 0.383 0.734 0.348 0.822 1.011 0.275 0.770 0.402 0.345 0.739 0.539 0.253 0.631 0.748 0.107 1.381 1.574 0.343 1.105 1.000 0.184 2.353 1.493 0.232 2.492 1.127 0.133 1.423 0.553 0.107 0.955 0.708 0.097 Asparagine 2.483 0.534 0.467 0.688 1.412 1.066 2.645 1.853 0.609 0.966 0.404 0.593 0.930 0.747 0.896 0.632 0.725 0.492 1.699 1.602 0.870 0.496 0.662 0.363 0.864 2.594 1.054 1.670 2.130 0.486 0.916 1.333 0.328 0.801 0.626 0.109 Aspartate 3.178 0.549 0.452 0.594 1.130 1.140 1.360 1.461 0.580 0.852 0.595 0.451 0.609 0.406 0.616 0.849 0.712 0.457 1.033 1.265 1.028 0.489 0.639 0.335 1.632 2.236 1.523 0.739 2.480 0.674 1.210 1.333 0.326 0.966 0.522 0.182 Betaine 0.191 0.190 0.370 0.262 0.612 0.972 0.656 0.491 0.125 0.545 0.128 0.360 0.329 0.212 0.390 0.156 0.230 0.184 0.300 0.313 0.207 0.129 0.146 0.165 2.353 0.296 0.480 0.282 0.563 0.558 0.276 0.154 0.101 0.369 0.044 0.119 Citruline 1.827 0.697 0.353 0.349 0.990 0.284 0.355 1.680 0.186 0.887 0.659 0.212 0.927 0.776 0.381 0.806 0.749 0.073 1.603 1.659 0.156 1.427 1.127 0.187 1.165 2.350 1.021 2.798 1.255 0.279 1.637 0.554 0.137 1.061 0.935 0.082 Glutamine 2.091 0.854 0.364 0.501 1.089 0.767 1.718 1.762 0.556 0.812 0.440 0.519 0.985 0.774 0.474 0.843 1.149 0.235 1.980 1.763 0.487 0.894 1.074 0.244 1.164 1.782 0.613 1.994 1.721 0.360 0.929 0.904 0.235 1.262 0.326 0.146 Histidine 2.641 1.077 0.569 0.479 1.092 1.125 2.424 1.053 0.663 0.607 0.345 0.538 0.563 0.680 1.242 0.438 0.439 0.531 1.833 0.826 1.227 2.015 0.516 0.601 1.049 2.204 1.672 2.798 1.830 2.063 1.430 1.258 0.582 1.043 1.528 0.568 Homoserine 1.792 1.444 1.172 1.232 2.572 2.100 3.562 0.746 0.954 2.075 0.716 1.519 0.590 0.371 1.689 0.589 0.683 1.033 1.022 1.117 2.850 0.468 0.468 1.225 1.307 2.254 2.799 1.086 2.795 1.163 0.802 0.859 0.998 0.549 1.081 0.850 Isoleucine 1.158 0.591 0.927 1.047 2.247 2.201 4.756 0.065 0.677 1.788 0.452 1.190 0.443 0.313 1.560 0.343 0.470 0.797 0.676 0.734 2.313 0.213 0.381 1.128 1.546 1.829 2.917 1.369 2.539 1.322 0.497 0.400 0.749 0.373 0.708 0.499 Kynurenine 2.265 0.357 0.414 1.153 1.754 1.429 4.756 0.106 1.100 1.977 0.699 1.480 0.713 0.566 0.976 0.502 0.544 1.082 0.549 2.468 0.342 1.452 1.549 2.063 3.299 1.012 0.490 1.151 0.588 0.402 L-Aminoadipate 0.807 0.174 0.818 0.911 1.637 1.892 3.015 0.065 0.691 1.148 0.692 1.122 1.151 0.687 1.469 0.633 0.823 0.650 1.656 1.763 1.675 0.281 0.849 0.798 1.394 2.312 2.894 1.529 2.068 1.811 0.645 0.736 0.810 0.585 0.636 0.368 Leucine 2.386 0.287 0.348 0.607 1.402 2.202 4.046 0.073 0.759 1.502 0.322 1.146 0.450 0.285 1.767 0.341 0.503 1.102 0.678 0.854 2.999 0.360 0.452 1.470 1.184 2.073 4.047 1.288 2.902 2.114 0.467 0.656 1.102 0.443 1.014 0.545 Methionine 1.577 0.284 0.436 1.004 2.190 2.059 3.412 0.082 0.668 2.075 0.530 1.321 0.953 0.577 1.667 0.746 1.014 0.789 0.862 1.120 2.616 0.548 0.811 1.144 1.505 2.538 2.301 2.239 1.890 1.253 0.601 0.607 0.855 0.669 0.723 0.436 Phenylalanine 1.977 0.586 0.223 0.396 0.713 0.640 2.191 0.373 0.577 1.328 0.361 0.244 0.199 0.288 0.741 0.317 0.419 0.276 0.530 0.751 1.718 0.634 0.553 0.953 1.730 2.679 1.799 1.351 1.917 0.537 2.056 0.553 0.570 0.306 0.491 0.167 Proline 0.669 0.237 0.315 0.384 0.786 0.595 4.756 0.099 0.419 0.902 0.408 0.793 0.482 0.345 0.446 0.498 0.616 0.308 0.739 0.933 1.005 0.329 0.595 0.495 1.401 1.481 0.877 0.711 2.993 0.503 0.558 0.462 0.308 0.419 0.608 0.321 S-Adenosyl-L-homocysteine 2.762 0.553 0.569 1.001 2.043 1.227 2.478 2.292 1.074 1.273 0.732 1.055 1.102 0.694 1.558 0.895 1.210 0.779 1.080 1.060 2.176 0.610 0.911 0.800 1.442 2.679 0.881 2.495 2.434 1.266 0.936 1.229 0.716 1.258 0.311 0.101 Sarcosine 1.991 1.009 0.652 0.558 1.283 1.032 0.584 1.287 0.253 0.924 0.377 0.647 0.756 0.693 0.715 0.641 0.715 0.321 1.502 1.448 0.563 1.284 1.013 0.250 2.217 2.443 0.674 2.798 1.979 0.500 1.617 0.594 0.120 1.094 1.036 0.171 Serine 3.621 1.825 1.104 1.235 2.518 1.553 3.556 2.955 0.899 1.534 0.945 1.162 0.846 0.655 1.200 0.739 0.849 0.672 1.286 1.489 1.650 1.712 0.798 0.853 2.353 2.425 1.795 2.522 3.139 1.299 1.435 0.995 0.908 0.727 1.019 0.571 Threonine 2.515 1.413 0.804 0.702 1.290 1.045 1.400 2.265 0.498 1.039 0.830 1.691 1.085 0.770 0.844 0.972 1.391 1.042 1.722 1.710 2.452 1.185 1.183 1.166 1.714 2.430 2.089 2.517 2.608 0.885 1.102 1.023 0.698 1.261 0.605 0.373 Tryptophan 0.139 0.285 0.759 0.889 2.040 1.371 1.512 0.123 0.659 1.393 0.513 1.226 0.911 0.661 1.215 0.805 1.285 0.739 1.465 1.763 2.367 0.374 0.846 1.113 1.788 2.435 2.708 1.753 3.103 2.275 1.054 0.972 1.234 0.644 1.093 0.572 Tyrosine 1.181 0.163 0.452 0.544 1.118 1.038 2.333 0.090 0.634 1.819 0.321 0.931 0.572 0.443 0.815 0.515 0.677 0.639 0.800 1.053 1.879 0.849 0.697 1.291 2.290 2.679 2.293 1.473 4.299 1.379 1.602 0.844 1.273 0.833 0.246 0.625 Valine 3.450 0.703 1.086 1.291 2.685 2.236 3.880 0.118 0.730 1.771 0.588 1.308 0.690 0.524 1.334 0.725 0.962 0.924 0.993 1.339 2.925 0.441 0.687 1.211 2.353 2.130 3.424 1.762 3.760 1.072 1.162 0.623 0.922 0.606 0.558 0.534 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name quanitfied m/z PubChemID KEGG ID Alanine 90.05495 5950 C00041 Asparagine 133.06076 236 C00152 Aspartate 134.04478 424 C16433 Betaine 118.08625 247 C00719 Citruline 176.10295 9750 D07706 Glutamine 148.06043 5961 C00303 Histidine 156.07674 6274 D00032 Homoserine 120.06551 779 C00263 Isoleucine 132.1019 791 D00065 Kynurenine 209.09206 161166 C00328 L-Aminoadipate 162.07608 6992111 C00956 Leucine 132.1019 6106 D00030 Methionine 150.05832 6147 C00073 Phenylalanine 166.08625 6140 D00021 Proline 116.0706 145742 C00148 S-Adenosyl-L-homocysteine 385.12884 439155 C00021 Sarcosine 90.05495 1088 C00213 Serine 106.04986 5951 C00065 Threonine 120.06551 6288 D00041 Tryptophan 205.09715 6305 D00020 Tyrosine 182.08116 6057 C00082 Valine 118.08625 6287 D00039 METABOLITES_END #END