#METABOLOMICS WORKBENCH ljx_20230310_001741 DATATRACK_ID:3783 STUDY_ID:ST002395 ANALYSIS_ID:AN004119
VERSION                          	1
CREATED_ON                       	03-20-2023
#PROJECT
PR:PROJECT_TITLE                 	A Mammalian Conserved Circular RNA CircLARP2 Regulates Hepatocellular Carcinoma
PR:PROJECT_TITLE                 	Metastasis and Lipid Metabolism
PR:PROJECT_SUMMARY               	Circular RNAs (circRNAs) have emerged as crucial regulators in physiology and
PR:PROJECT_SUMMARY               	human diseases. However, evolutionarily conserved circRNAs with potent functions
PR:PROJECT_SUMMARY               	in cancers are rarely reported. Here, we identified a mammalian conserved
PR:PROJECT_SUMMARY               	circRNA circLARP2 that played critical roles in hepatocellular carcinoma (HCC).
PR:PROJECT_SUMMARY               	With clinical specimens, we found that patients with high circLARP2 levels in
PR:PROJECT_SUMMARY               	HCC had advanced prognostic stage and poor overall survival. CircLARP2
PR:PROJECT_SUMMARY               	facilitated HCC metastasis and lipid accumulation in HCC cell lines. CircLARP2
PR:PROJECT_SUMMARY               	was one of the rare ones that were identified in HCC metastasis and conserved in
PR:PROJECT_SUMMARY               	mammals, which enabled further studies with animal models. CircLARP2-deficient
PR:PROJECT_SUMMARY               	mice exhibited reduced metastasis and less lipid accumulation in an induced HCC
PR:PROJECT_SUMMARY               	model. We provided lines of evidence at molecular, cellular, and whole
PR:PROJECT_SUMMARY               	organismal levels, to support that circLARP2 functioned as a protein sponge of
PR:PROJECT_SUMMARY               	AUF1. CircLARP2 sequestered AUF1 from binding to LKB1 mRNA, which led to
PR:PROJECT_SUMMARY               	decreased LKB1 mRNA stability and lower LKB1 protein levels. LKB1 as a kinase
PR:PROJECT_SUMMARY               	promoted the phosphorylation of AMPK and then the phosphorylation of ACC, the
PR:PROJECT_SUMMARY               	rate limiting enzyme of fatty acid synthesis. Knockdown of Lkb1 with AAV8-shLkb1
PR:PROJECT_SUMMARY               	in mice HCC model also proved that Lkb1 was a key element in the regulation.
PR:PROJECT_SUMMARY               	Through this AUF1-LKB1-AMPK-ACC pathway, circLARP2 promoted HCC metastasis and
PR:PROJECT_SUMMARY               	lipid accumulation.
PR:INSTITUTE                     	University of Science and Technology of China
PR:LAST_NAME                     	Li
PR:FIRST_NAME                    	Jingxin
PR:ADDRESS                       	443 Huangshan Road
PR:EMAIL                         	ljx0418@mail.ustc.edu.cn
PR:PHONE                         	00-86-0551-63600137
PR:DOI                           	http://dx.doi.org/10.21228/M8CM5D
#STUDY
ST:STUDY_TITLE                   	A Mammalian Conserved Circular RNA CircLARP2 Regulates Hepatocellular Carcinoma
ST:STUDY_TITLE                   	Metastasis and Lipid Metabolism (Part 2)
ST:STUDY_SUMMARY                 	Circular RNAs (circRNAs) have emerged as crucial regulators in physiology and
ST:STUDY_SUMMARY                 	human diseases. However, evolutionarily conserved circRNAs with potent functions
ST:STUDY_SUMMARY                 	in cancers are rarely reported. Here, we identified a mammalian conserved
ST:STUDY_SUMMARY                 	circRNA circLARP2 that played critical roles in hepatocellular carcinoma (HCC).
ST:STUDY_SUMMARY                 	With clinical specimens, we found that patients with high circLARP2 levels in
ST:STUDY_SUMMARY                 	HCC had advanced prognostic stage and poor overall survival. CircLARP2
ST:STUDY_SUMMARY                 	facilitated HCC metastasis and lipid accumulation in HCC cell lines. CircLARP2
ST:STUDY_SUMMARY                 	was one of the rare ones that were identified in HCC metastasis and conserved in
ST:STUDY_SUMMARY                 	mammals, which enabled further studies with animal models. CircLARP2-deficient
ST:STUDY_SUMMARY                 	mice exhibited reduced metastasis and less lipid accumulation in an induced HCC
ST:STUDY_SUMMARY                 	model. We provided lines of evidence at molecular, cellular, and whole
ST:STUDY_SUMMARY                 	organismal levels, to support that circLARP2 functioned as a protein sponge of
ST:STUDY_SUMMARY                 	AUF1. CircLARP2 sequestered AUF1 from binding to LKB1 mRNA, which led to
ST:STUDY_SUMMARY                 	decreased LKB1 mRNA stability and lower LKB1 protein levels. LKB1 as a kinase
ST:STUDY_SUMMARY                 	promoted the phosphorylation of AMPK and then the phosphorylation of ACC, the
ST:STUDY_SUMMARY                 	rate limiting enzyme of fatty acid synthesis. Knockdown of Lkb1 with AAV8-shLkb1
ST:STUDY_SUMMARY                 	in mice HCC model also proved that Lkb1 was a key element in the regulation.
ST:STUDY_SUMMARY                 	Through this AUF1-LKB1-AMPK-ACC pathway, circLARP2 promoted HCC metastasis and
ST:STUDY_SUMMARY                 	lipid accumulation.
ST:INSTITUTE                     	University of Science and Technology of China
ST:LAST_NAME                     	Li
ST:FIRST_NAME                    	Jingxin
ST:ADDRESS                       	443 Huangshan Road, HeFei, AnHui, 230022, China
ST:EMAIL                         	ljx0418@mail.ustc.edu.cn
ST:PHONE                         	00-86-0551-63600137
ST:SUBMIT_DATE                   	2023-03-10
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	KO-1	Genotype:circLARP2-deficiency	RAW_FILE_NAME=KO-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KO-2	Genotype:circLARP2-deficiency	RAW_FILE_NAME=KO-2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KO-3	Genotype:circLARP2-deficiency	RAW_FILE_NAME=KO-3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	WT-1	Genotype:Wild-type	RAW_FILE_NAME=WT-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	WT-2	Genotype:Wild-type	RAW_FILE_NAME=WT-2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	WT-3	Genotype:Wild-type	RAW_FILE_NAME=WT-3.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	500 μL cold extraction buffer (methanol: acetonitrile: water = 2:2:1, v/v/v)
CO:COLLECTION_SUMMARY            	was added to each sample. Samples were sonicated for 2 minutes and centrifuged
CO:COLLECTION_SUMMARY            	at 14,000 g for 5 min at 4 °C. Supernatants were thoroughly lyophilized
CO:COLLECTION_SUMMARY            	(FreeZone 6 Liter, Labconco, USA) and reconstituted in 50 μL of methanol-water
CO:COLLECTION_SUMMARY            	(1:1, v/v) just prior to measurement.
CO:SAMPLE_TYPE                   	Cultured cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Twenty-four hours after seeding, cells were washed three times with 0.9% NaCl
TR:TREATMENT_SUMMARY             	and incubated in DMEM without glucose, glutamine or sodium pyruvate supplemented
TR:TREATMENT_SUMMARY             	with 15 mM [U-13C] glucose, 4 mM [U-13C] glutamine, 10% FBS and the
TR:TREATMENT_SUMMARY             	indicated treatment condition for 24 h before metabolite extraction.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Supernatants were thoroughly lyophilized (FreeZone 6 Liter, Labconco, USA) and
SP:SAMPLEPREP_SUMMARY            	reconstituted in 50 μL of methanol-water (1:1, v/v) just prior to measurement.
#CHROMATOGRAPHY
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	SeQuant ZIC-HILIC (100 x 2.1mm,3.5um)
CH:COLUMN_TEMPERATURE            	45
CH:FLOW_GRADIENT                 	The initial mobile phase was 30% solvent B at a flow rate of 300 μL/min. It was
CH:FLOW_GRADIENT                 	held for 2 min, and then linearly increased to 100% solvent B in 23 min,
CH:FLOW_GRADIENT                 	followed by equilibrating at 5% solvent B for 10 min.
CH:FLOW_RATE                     	300 μL/min
CH:SOLVENT_A                     	acetonitrile–water (6:4, v/v) with 0.1% formic acid and 0.1mM ammonium formate
CH:SOLVENT_B                     	acetonitrile–isopropanol (1:9, v/v) with 0.1% formic acid and 0.1 mM ammonium
CH:SOLVENT_B                     	formate
CH:CHROMATOGRAPHY_TYPE           	HILIC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Plus Orbitrap
MS:INSTRUMENT_TYPE               	Triple TOF
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	Data processing and ion annotation based on accurate mass were performed in
MS:MS_COMMENTS                   	TraceFinder 5.0 (Thermo Fisher) and Xcalibur 4.0 (Thermo Fisher).
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	peak area
MS_METABOLITE_DATA_START
Samples	KO-1	KO-2	KO-3	WT-1	WT-2	WT-3
Factors	Genotype:circLARP2-deficiency	Genotype:circLARP2-deficiency	Genotype:circLARP2-deficiency	Genotype:Wild-type	Genotype:Wild-type	Genotype:Wild-type	
Arachidic acid m+0	65.8199	62.0970	66.5744	43.3174	46.3954	46.0486
Arachidic acid m+10	1.7447	2.9545	3.3207	3.3810	4.0294	3.5015
Arachidic acid m+12	5.9175	6.3429	5.8194	6.9783	6.4080	6.5889
Arachidic acid m+14	4.5316	3.8269	2.7695	8.1654	8.1786	8.3091
Arachidic acid m+16	2.9699	2.8846	3.2421	7.5937	6.7301	6.3663
Arachidic acid m+18	0.8834	1.2147	0.7688	4.0821	3.6477	3.7712
Arachidic acid m+2	11.4655	13.0192	12.9955	15.9532	13.9158	14.7085
Arachidic acid m+20				0.2139	0.7003	0.5431
Arachidic acid m+4	4.2366	4.3463	2.2295	6.8302	6.6682	6.4753
Arachidic acid m+6		0.2557	0.3079	0.0736	0.2354	0.2260
Arachidic acid m+8	1.1776	2.0451	1.0580	0.5822	1.2343	1.5511
Behenic acid m+0	72.7991	70.9511	72.5679	56.0235	57.8558	64.8660
Behenic acid m+10	1.8372	2.1694	2.1757	1.5921	1.6857	1.2580
Behenic acid m+12	2.8366	3.2413	2.9141	3.1265	2.9179	2.6569
Behenic acid m+14	3.2540	3.5469	3.0627	4.7083	4.6664	4.1219
Behenic acid m+16	2.3481	2.9471	2.4461	5.3185	4.9781	4.0990
Behenic acid m+18	1.3168	1.5926	1.2342	3.8497	3.4414	3.1029
Behenic acid m+2	6.0757	5.8685	5.4711	7.5152	7.3754	6.2757
Behenic acid m+20	0.3991	0.4002	0.4642	1.7919	1.7086	1.5443
Behenic acid m+4	5.5407	5.7415	5.5032	9.0081	8.5990	6.8428
Behenic acid m+6	1.7979	1.6870	1.8192	3.1103	3.1250	2.5733
Behenic acid m+8	0.9956	1.1486	1.0380	0.6050	0.6621	0.4115
Docosadienoic acid m+0	50.3717	51.3162	49.1392	38.8793	37.1196	43.5271
Docosadienoic acid m+10	0.0000	0.0000				
Docosadienoic acid m+12	1.7091	0.8399		0.9313	1.3221	0.9308
Docosadienoic acid m+14				3.4237	1.6143	2.9345
Docosadienoic acid m+16				1.6043	2.1450	2.5687
Docosadienoic acid m+2	32.0852	32.1900	38.4310	30.8112	30.3589	29.3413
Docosadienoic acid m+4	15.8335	15.6536	12.4298	22.5744	26.1681	18.8874
Docosadienoic acid m+6				1.7219	1.2717	1.8100
Docosapentaenoic acid m+0	95.9877	96.4198	95.7934	92.8152	92.9969	92.8188
Docosapentaenoic acid m+2	4.0123	3.5802	4.2066	7.1724	7.0031	7.0893
Docosapentaenoic acid m+4				0.0124		0.0919
Docosatetraenoic acid m+0	89.4519	87.9756	89.5904	84.7439	85.6711	86.2535
Docosatetraenoic acid m+2	10.5481	12.0244	10.4096	14.0600	14.3289	13.7072
Docosatetraenoic acid m+4						0.0393
Eicosadienoic acid	71.4436	87.2583	71.4605	54.4599	60.1163	62.2680
Eicosadienoic acid m+2	28.5564	12.7417	27.7665	39.8768	37.7386	34.0270
Eicosadienoic acid m+4				3.1803	2.1451	3.7050
Eicosatrienoic acid m+0	96.0282	96.9076	98.9448	91.4184	92.5887	92.5911
Eicosatrienoic acid m+2	3.9718	3.0924	1.0552	7.5379	7.4113	6.1461
Eicosatrienoic acid m+4				0.5648		0.8252
Elaidic acid m+0	47.6735	77.5919	65.1585	29.9935	47.5856	39.4325
Elaidic acid m+10	6.2720	6.2173	6.9950	8.3009	6.7820	6.0331
Elaidic acid m+12	7.8263	4.6426	9.6330	9.8475	10.8726	10.9179
Elaidic acid m+14	6.0465	3.7098	2.2896	13.1404	8.5400	9.9087
Elaidic acid m+16	1.5618	0.2796	0.8469	7.0270	4.9242	6.4620
Elaidic acid m+18				0.9703	0.8393	0.8477
Elaidic acid m+2	7.5271	6.2594		14.3848	10.4586	12.4314
Erucic acid m+0	65.5746	65.2230	62.8606	60.8227	52.8513	63.3623
Erucic acid m+10	0.6315	0.6280	0.6920	0.3922	0.5672	0.5038
Erucic acid m+12	1.8811	2.4804	2.2788	1.3917	0.8220	0.9343
Erucic acid m+14	1.4509	1.0026	1.5415	1.4475	2.1346	0.8910
Erucic acid m+16	1.0421	0.5290	0.3044	1.1241	1.2841	0.8339
Erucic acid m+18						0.8627
Erucic acid m+2	15.7412	16.1679	16.8722	15.2625	20.1915	15.3309
Erucic acid m+20						0.1124
Erucic acid m+4	13.1158	13.5762	14.6922	16.2606	19.8858	15.7771
Erucic acid m+6	0.5629	0.3929	0.7583	3.1736	2.1785	1.3729
Gadoleic acid m+0	48.7170	55.8980	45.1380	37.8368	38.0743	40.0472
Gadoleic acid m+10	0.7519	0.7869	1.6034	1.1660	0.8067	0.8674
Gadoleic acid m+12	3.0733	3.2521	3.3900	3.5016	2.9673	3.1211
Gadoleic acid m+14	2.5287	2.2990	2.8314	4.3722	4.1261	3.7563
Gadoleic acid m+16	0.5682	1.0135	1.6278	3.3396	2.7269	3.2553
Gadoleic acid m+18	0.1079	0.4008	0.6410	1.8214	1.9058	1.4514
Gadoleic acid m+2	0.1390	0.2527	0.2563	0.7172	0.0497	0.1806
Gadoleic acid m+20				0.1171	0.1741	0.2440
Gadoleic acid m+8		0.0941	0.0744	0.0967		0.0366
Hexadecenoic acid	88.1443	87.0269	88.1733	75.9693	81.5469	78.1907
Hexadecenoic acid m+10	3.4771	3.5374	2.4253	6.5009	3.2678	6.4559
Hexadecenoic acid m+12	2.2124	3.1204	2.7196	7.5002	4.9381	6.4292
Hexadecenoic acid m+14	2.1235	1.0922	2.0430	4.1131	4.7119	3.4006
Hexadecenoic acid m+16				0.7182	0.5108	0.6723
Hexadecenoic acid m+6	1.0522	0.7832	1.2120	1.5617	0.8010	0.6728
Hexadecenoic acid m+8	2.9899	4.2129	3.4259	3.0378	4.2229	3.4815
Lignoceric acid m+0	56.0714	54.7364	56.5335	47.1869	42.8841	53.1485
Lignoceric acid m+10	1.8535	1.9834	1.9400	1.1042	1.2325	0.8864
Lignoceric acid m+12	3.1120	3.3015	3.0641	2.1459	2.4776	2.0353
Lignoceric acid m+14	4.2866	4.3071	3.9791	3.8219	4.0577	3.3120
Lignoceric acid m+15	0.6182	0.6980	0.6955	0.7980	0.9275	0.8184
Lignoceric acid m+16	4.1002	4.0834	4.0210	5.1115	5.3970	4.5453
Lignoceric acid m+18	3.0053	2.9503	2.9185	4.8138	5.2636	4.3938
Lignoceric acid m+2	7.5486	7.7162	7.2017	7.4392	8.0864	6.7117
Lignoceric acid m+20	1.3875	1.6318	1.5791	3.4197	3.6751	2.9042
Lignoceric acid m+22	0.3657	0.3439	0.4579	1.5449	1.5115	1.2332
Lignoceric acid m+4	7.8304	7.8380	7.5953	8.5802	8.9215	7.5846
Lignoceric acid m+6	5.5297	5.4533	5.2827	7.5255	8.0824	6.5205
Lignoceric acid m+8	2.1034	2.0787	2.1338	2.5903	2.9522	2.3913
Myristic acid m+0	58.1334	84.0504	80.0151	56.6436	61.5555	60.9646
Myristic acid m+10	22.4325		19.9838	18.5302	17.4540	17.0129
Myristic acid m+12				8.2633	10.6253	13.7874
Myristic acid m+14				7.2644		
Myristic acid m+6	8.6938	4.5483			3.9026	5.9551
Myristic acid m+8	10.7370	11.3996		9.2969	6.4611	2.2777
Nervonic acid m+0	56.5531	56.2379	58.1945	47.6533	48.8495	48.7953
Nervonic acid m+10	0.8610	0.8149	0.6641	0.5883	0.4985	0.4288
Nervonic acid m+12	1.1598	1.5964	1.2080	1.0798	0.9908	1.1732
Nervonic acid m+14	1.7365	1.8387	1.7834	1.9122	1.6999	1.6363
Nervonic acid m+16	1.7184	1.7930	1.7997	2.3762	2.0581	2.1264
Nervonic acid m+18	1.1692	1.2862	1.1736	2.1285	2.0240	2.0064
Nervonic acid m+2	11.9589	11.3264	10.9085	10.7165	10.5521	10.8899
Nervonic acid m+20	0.5800	0.5328	0.5893	1.3967	1.3210	1.2842
Nervonic acid m+22	0.1111	0.1042	0.0692	0.5475	0.5818	0.5593
Nervonic acid m+4	13.9998	14.2589	13.3925	16.0741	15.7829	15.6569
Nervonic acid m+6	7.7044	7.9750	7.7208	11.3580	11.2636	11.0592
Nervonic acid m+8	1.3689	1.2267	1.1477	1.7476	1.7256	1.8142
Oleic acid m+0	82.0465	81.6604	81.3792	73.7074	75.3170	77.0943
Oleic acid m+10	2.6794	3.0485	3.0223	3.1051	2.7757	2.6187
Oleic acid m+12	2.8552	3.0510	3.0023	4.1761	3.9399	3.5684
Oleic acid m+14	2.0217	2.0133	2.0815	3.8133	3.6346	3.2222
Oleic acid m+16	0.8564	0.7887	0.8506	2.1195	2.0013	1.8493
Oleic acid m+18	0.1232	0.0681	0.0719	0.4605	0.5221	0.4403
Oleic acid m+2	4.7757	4.8925	5.2923	7.6498	7.4672	6.7156
Oleic acid m+4	0.3440	0.3583	0.3561	0.3498	0.2312	0.3462
Oleic acid m+6	0.8973	0.9824	0.9797	0.7524	0.6388	0.6900
Oleic acid m+8	1.8849	1.9931	2.0122	1.7403	1.5739	1.4408
Palmitic acid m+0	70.9766	63.9859	67.6461	58.5571	66.8111	59.1641
Palmitic acid m+10	7.6744	9.2083	8.3894	9.1134	7.8796	8.6670
Palmitic acid m+12	6.3616	7.3204	7.4249	10.5004	8.0840	10.3177
Palmitic acid m+14	2.9286	3.4646	3.0830	6.9670	5.5124	6.5833
Palmitic acid m+16	0.5471	0.6447	0.3886	1.8949	1.5959	2.0379
Palmitic acid m+2	0.0823	0.3836	0.1340	0.2869	0.1496	0.3804
Palmitic acid m+4	0.7638	1.3523	1.0300	0.8306	0.5410	0.7371
Palmitic acid m+6	3.1021	3.9155	3.0621	2.5246	1.9796	2.2787
Palmitic acid m+8	6.0987	7.3789	6.4637	5.5716	4.3848	5.5003
Stearic acid m+0	86.4611	82.2308	87.2806	72.5323	82.5727	71.4936
Stearic acid m+10	2.4761	3.1931	2.1780	3.7191	2.2027	3.6891
Stearic acid m+12	2.5763	3.2848	2.5746	4.9596	3.2830	5.2380
Stearic acid m+14	1.8674	2.3766	1.6905	4.6793	3.0563	4.8711
Stearic acid m+16	0.6335	0.9435	0.6694	2.7575	1.6659	2.7230
Stearic acid m+18	0.0795	0.0614	0.1033	0.7050	0.4507	0.6710
Stearic acid m+2	2.4265	3.4213	2.2837	5.2366	3.4549	5.5287
Stearic acid m+4	0.1883	0.3881	0.1883	0.3378	0.0591	0.3061
Stearic acid m+6	0.7118	0.9308	0.7484	0.8199	0.4515	0.7626
Stearic acid m+8	1.6159	2.2305	1.3734	1.7984	1.1808	1.9393
Tricosanoic acid m+0	98.6126	99.4333	100.0000	99.7381	100.0000	100.0000
Tricosanoic acid m+2	1.3874	0.5667		0.2619
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
Arachidic acid m+0						8.42		311.29554999999999	
Arachidic acid m+10								321.32905000000011	
Arachidic acid m+12								323.33575000000013	
Arachidic acid m+14								325.34245000000016	
Arachidic acid m+16								327.34915000000018	
Arachidic acid m+18								329.3558500000002	
Arachidic acid m+2								313.30225000000002	
Arachidic acid m+20								331.36255000000023	
Arachidic acid m+4								315.30895000000004	
Arachidic acid m+6								317.31565000000006	
Arachidic acid m+8								319.32235000000009	
Behenic acid m+0						9.1999999999999993		339.32684999999998	
Behenic acid m+10								349.3603500000001	
Behenic acid m+12								351.36705000000012	
Behenic acid m+14								353.37375000000014	
Behenic acid m+16								355.38045000000017	
Behenic acid m+18								357.38715000000019	
Behenic acid m+2								341.33355	
Behenic acid m+20								359.39385000000021	
Behenic acid m+4								343.34025000000003	
Behenic acid m+6								345.34695000000005	
Behenic acid m+8								347.35365000000007	
Docosadienoic acid m+0						7.85		335.29554999999999	
Docosadienoic acid m+10								345.32905000000011	
Docosadienoic acid m+12								347.33575000000013	
Docosadienoic acid m+14								349.34245000000016	
Docosadienoic acid m+16								351.34915000000018	
Docosadienoic acid m+2								337.30225000000002	
Docosadienoic acid m+4								339.30895000000004	
Docosadienoic acid m+6								341.31565000000006	
Docosapentaenoic acid m+0						6.52		329.24860000000001	
Docosapentaenoic acid m+2								331.25530000000003	
Docosapentaenoic acid m+4								333.26200000000006	
Docosatetraenoic acid m+0						6.83		331.26425	
Docosatetraenoic acid m+2								333.27095000000003	
Docosatetraenoic acid m+4								335.27765000000005	
Eicosadienoic acid						7.13		307.26425	
Eicosadienoic acid m+2								309.27095000000003	
Eicosadienoic acid m+4								311.27765000000005	
Eicosatrienoic acid m+0						6.59		305.24860000000001	
Eicosatrienoic acid m+2								307.25530000000003	
Eicosatrienoic acid m+4								309.26200000000006	
Elaidic acid m+0						7.11		281.24860000000001	
Elaidic acid m+10								291.28210000000013	
Elaidic acid m+12								293.28880000000015	
Elaidic acid m+14								295.29550000000017	
Elaidic acid m+16								297.3022000000002	
Elaidic acid m+18								299.30890000000022	
Elaidic acid m+2								283.25530000000003	
Erucic acid m+0						8.4		337.31119999999999	
Erucic acid m+10								347.34670000000017	
Erucic acid m+12								349.35380000000021	
Erucic acid m+14								351.36090000000024	
Erucic acid m+16								353.36800000000028	
Erucic acid m+18								355.37510000000032	
Erucic acid m+2								339.31830000000002	
Erucic acid m+20								357.38220000000035	
Erucic acid m+4								341.32540000000006	
Erucic acid m+6								343.3325000000001	
Gadoleic acid m+0						7.74		309.2799	
Gadoleic acid m+10								319.31340000000012	
Gadoleic acid m+12								321.32010000000014	
Gadoleic acid m+14								323.32680000000016	
Gadoleic acid m+16								325.33350000000019	
Gadoleic acid m+18								327.34020000000021	
Gadoleic acid m+2								311.28660000000002	
Gadoleic acid m+20								329.34690000000023	
Gadoleic acid m+8								317.30670000000009	
Hexadecenoic acid						6.14		253.21729999999999	
Hexadecenoic acid m+10								263.25082000000009	
Hexadecenoic acid m+12								265.25752000000011	
Hexadecenoic acid m+14								267.26422000000014	
Hexadecenoic acid m+16								269.27092000000016	
Hexadecenoic acid m+6								259.23742000000004	
Hexadecenoic acid m+8								261.24412000000007	
Lignoceric acid m+0						9.6		367.35814999999997	
Lignoceric acid m+10								377.39165000000008	
Lignoceric acid m+12								379.39835000000011	
Lignoceric acid m+14								381.40505000000013	
Lignoceric acid m+15								382.40840000000014	
Lignoceric acid m+16								383.41175000000015	
Lignoceric acid m+18								385.41845000000018	
Lignoceric acid m+2								369.36484999999999	
Lignoceric acid m+20								387.4251500000002	
Lignoceric acid m+22								389.43185000000022	
Lignoceric acid m+4								371.37155000000001	
Lignoceric acid m+6								373.37825000000004	
Lignoceric acid m+8								375.38495000000006	
Myristic acid m+0						6		227.20165	
Myristic acid m+10								237.23515000000012	
Myristic acid m+12								239.24185000000014	
Myristic acid m+14								241.24855000000017	
Myristic acid m+6								233.22175000000007	
Myristic acid m+8								235.22845000000009	
Nervonic acid m+0						8.98		365.34249999999997	
Nervonic acid m+10								375.37600000000009	
Nervonic acid m+12								377.38270000000011	
Nervonic acid m+14								379.38940000000014	
Nervonic acid m+16								381.39610000000016	
Nervonic acid m+18								383.40280000000018	
Nervonic acid m+2								367.3492	
Nervonic acid m+20								385.40950000000021	
Nervonic acid m+22								387.41620000000023	
Nervonic acid m+4								369.35590000000002	
Nervonic acid m+6								371.36260000000004	
Nervonic acid m+8								373.36930000000007	
Oleic acid m+0						6.98		281.24860000000001	
Oleic acid m+10								291.28210000000013	
Oleic acid m+12								293.28880000000015	
Oleic acid m+14								295.29550000000017	
Oleic acid m+16								297.3022000000002	
Oleic acid m+18								299.30890000000022	
Oleic acid m+2								283.25530000000003	
Oleic acid m+4								285.26200000000006	
Oleic acid m+6								287.26870000000008	
Oleic acid m+8								289.2754000000001	
Palmitic acid m+0						6.89		255.23294999999999	
Palmitic acid m+10								265.26645000000008	
Palmitic acid m+12								267.2731500000001	
Palmitic acid m+14								269.27985000000012	
Palmitic acid m+16								271.28655000000015	
Palmitic acid m+2								257.23964999999998	
Palmitic acid m+4								259.24635000000001	
Palmitic acid m+6								261.25305000000003	
Palmitic acid m+8								263.25975000000005	
Stearic acid m+0						7.72		283.26425	
Stearic acid m+10								293.29775000000012	
Stearic acid m+12								295.30445000000014	
Stearic acid m+14								297.31115000000017	
Stearic acid m+16								299.31785000000019	
Stearic acid m+18								301.32455000000022	
Stearic acid m+2								285.27095000000003	
Stearic acid m+4								287.27765000000005	
Stearic acid m+6								289.28435000000007	
Stearic acid m+8								291.2910500000001	
Tricosanoic acid m+0						9.32		353.34249999999997	
Tricosanoic acid m+2								355.3492	
METABOLITES_END
#END