#METABOLOMICS WORKBENCH bur27_20230223_182126 DATATRACK_ID:3763 STUDY_ID:ST002499 ANALYSIS_ID:AN004106 PROJECT_ID:PR001272 VERSION 1 CREATED_ON March 2, 2023, 3:25 pm #PROJECT PR:PROJECT_TITLE Metabolic profiles of murine stress erythroid progenitors PR:PROJECT_SUMMARY Inflammation alters hematopoiesis, skewing production to generate myeloid PR:PROJECT_SUMMARY effector cells at the expense of steady state erythropoiesis. To compensate, PR:PROJECT_SUMMARY stress erythropoiesis is induced to maintain homeostasis until the inflammation PR:PROJECT_SUMMARY is resolved. Unlike the constant production of steady state erythropoiesis, PR:PROJECT_SUMMARY stress erythropoiesis generates a bolus of new erythrocytes by first producing PR:PROJECT_SUMMARY immature progenitor cells, which then transition to committed erythroid PR:PROJECT_SUMMARY progenitors and differentiate. We hypothesize that the proliferation of early PR:PROJECT_SUMMARY progenitor cells and their transition to differentiation is regulated by changes PR:PROJECT_SUMMARY in metabolism. Metabolomics and isotope tracing analysis was performed to assess PR:PROJECT_SUMMARY the intracellular metabolic profiles in proliferating progenitors isolated from PR:PROJECT_SUMMARY in vitro stress erythropoiesis cultures. We observed an active engagement of PR:PROJECT_SUMMARY glucose metabolism in glycolysis and anabolic biosynthesis, while the levels of PR:PROJECT_SUMMARY TCA intermediates suggested that TCA cycle and mitochondrial respiration were PR:PROJECT_SUMMARY blocked. Concomitantly, inducible nitric oxide synthase (iNOS) was induced in PR:PROJECT_SUMMARY progenitor cells to increase the production of nitric oxide (NO), which was PR:PROJECT_SUMMARY demonstrated to be crucial for proliferating progenitor metabolism. Inhibition PR:PROJECT_SUMMARY or genetic mutation of iNOS decreased NO levels resulting in the suppression of PR:PROJECT_SUMMARY progenitor proliferation in vitro and in vivo. As evaluated by RNA-seq, PR:PROJECT_SUMMARY inhibition of iNOS suppressed cell proliferation-related pathways including cell PR:PROJECT_SUMMARY cycle and nucleotide metabolism, while upregulating erythroid differentiation PR:PROJECT_SUMMARY genes. These data suggest that iNOS-derived NO production establishes a PR:PROJECT_SUMMARY metabolism that promotes the proliferation of progenitor cells while inhibiting PR:PROJECT_SUMMARY their differentiation. In contrast, the transition to differentiation is marked PR:PROJECT_SUMMARY by decreased Nos2 expression and a change in metabolism to support induction of PR:PROJECT_SUMMARY the erythroid gene expression program. These data support a model where PR:PROJECT_SUMMARY increased pro-inflammatory signals inhibit steady state erythropoiesis, while at PR:PROJECT_SUMMARY the same time promoting stress erythropoiesis to maintain homeostasis. PR:INSTITUTE Pennsylvania State University PR:DEPARTMENT Veterinary and Biomedical Sciences PR:LABORATORY Paulson Lab PR:LAST_NAME Ruan PR:FIRST_NAME Baiye PR:ADDRESS 228 AVBS Building, Shortlidge Road, University Park, PA 16802 PR:EMAIL bur27@psu.edu PR:PHONE 814-863-6306 #STUDY ST:STUDY_TITLE Metabolomics analysis of stress erythroid progenitors ST:STUDY_SUMMARY A time course study to assess the intracellular metabolic changes of splenic ST:STUDY_SUMMARY Kit+ stress erythroid progenitors ST:INSTITUTE Pennsylvania State University ST:DEPARTMENT Veterinary and Biomedical Sciences ST:LABORATORY Paulson Lab ST:LAST_NAME Ruan ST:FIRST_NAME Baiye ST:ADDRESS 228 AVBS Building Shortlidge Road University Park, PA 16802 ST:EMAIL bur27@psu.edu ST:PHONE 814-863-6306 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 0-1 Time_point(days):0 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=untreated; RAW_FILE_NAME=0-1.mzML SUBJECT_SAMPLE_FACTORS - 0-2 Time_point(days):0 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=untreated; RAW_FILE_NAME=0-2.mzML SUBJECT_SAMPLE_FACTORS - 0-3 Time_point(days):0 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=untreated; RAW_FILE_NAME=0-3.mzML SUBJECT_SAMPLE_FACTORS - 0-4 Time_point(days):0 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=untreated; RAW_FILE_NAME=0-4.mzML SUBJECT_SAMPLE_FACTORS - 0-5 Time_point(days):0 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=untreated; RAW_FILE_NAME=0-5.mzML SUBJECT_SAMPLE_FACTORS - 1-1 Time_point(days):1 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=1-1.mzML SUBJECT_SAMPLE_FACTORS - 1-2 Time_point(days):1 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=1-2.mzML SUBJECT_SAMPLE_FACTORS - 1-3 Time_point(days):1 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=1-3.mzML SUBJECT_SAMPLE_FACTORS - 1-4 Time_point(days):1 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=1-4.mzML SUBJECT_SAMPLE_FACTORS - 1-5 Time_point(days):1 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=1-5.mzML SUBJECT_SAMPLE_FACTORS - 3-1 Time_point(days):3 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=3-1.mzML SUBJECT_SAMPLE_FACTORS - 3-2 Time_point(days):3 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=3-2.mzML SUBJECT_SAMPLE_FACTORS - 3-3 Time_point(days):3 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=3-3.mzML SUBJECT_SAMPLE_FACTORS - 3-4 Time_point(days):3 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=3-4.mzML SUBJECT_SAMPLE_FACTORS - 3-5 Time_point(days):3 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=PHZ; RAW_FILE_NAME=3-5.mzML SUBJECT_SAMPLE_FACTORS - 8-1 Time_point(days):8 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=HKBA; RAW_FILE_NAME=8-1.mzML SUBJECT_SAMPLE_FACTORS - 8-2 Time_point(days):8 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=HKBA; RAW_FILE_NAME=8-2.mzML SUBJECT_SAMPLE_FACTORS - 8-3 Time_point(days):8 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=HKBA; RAW_FILE_NAME=8-3.mzML SUBJECT_SAMPLE_FACTORS - 8-4 Time_point(days):8 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=HKBA; RAW_FILE_NAME=8-4.mzML SUBJECT_SAMPLE_FACTORS - 8-5 Time_point(days):8 Cell_type=Kit+ stress progenitors; Organism=Mus musculus; Mouse_strain=wild type C57BL/6; Treatment=HKBA; RAW_FILE_NAME=8-5.mzML #COLLECTION CO:COLLECTION_SUMMARY Murine spleens were harvested and disassociated to single cell suspension at a CO:COLLECTION_SUMMARY concentration of 1 x 10^8 cells/mL. Labeling reagent was added at a concentation CO:COLLECTION_SUMMARY of 50 μL/mL of sample. Cells were mixed and incubated at RT for 15 mins. CO:COLLECTION_SUMMARY Selection cocktail was added to sample at a 70 μL/mL of sample. Cells were CO:COLLECTION_SUMMARY mixed and incubated at RT for 15 mins. RapidSpheres were added to sample at a 50 CO:COLLECTION_SUMMARY μL/mL of sample. Cells were mixed and incubated at RT for 10 mins. Cells were CO:COLLECTION_SUMMARY washed with FACS buffer for 4 times, and were resuspended for downstream CO:COLLECTION_SUMMARY metabolomics analysis. CO:SAMPLE_TYPE Stem cells #TREATMENT TR:TREATMENT_SUMMARY Phenylhydrazine (PHZ)-induced hemolytic anemia To induce acute hemolytic anemia, TR:TREATMENT_SUMMARY age- and sex-matched mice were injected intraperitoneally with a single dose TR:TREATMENT_SUMMARY (100 mg/kg body weight) of freshly prepared phenylhydrazine (Sigma-Aldrich, TR:TREATMENT_SUMMARY dissolved in PBS). Mice were sacrificed at indicated time points for spleen TR:TREATMENT_SUMMARY sample collection and Kit cell enrichment. Brucella abortus-induced inflammatory TR:TREATMENT_SUMMARY anemia Heat-killed Brucella abortus (HKBA, strain 1119-3) was used to induce TR:TREATMENT_SUMMARY anemia of inflammation following a previously described method(1). To induce TR:TREATMENT_SUMMARY stress erythropoiesis, age- and sex-matched mice were administered with 5 x 108 TR:TREATMENT_SUMMARY particles of HKBA via intraperitoneal injection.Mice were sacrificed at TR:TREATMENT_SUMMARY indicated time points for spleen sample collection and Kit cell enrichment. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cell pellets were extracted with 1 ml pre-chilled 50:50 HPLC-grade SP:SAMPLEPREP_SUMMARY water:methanol (v/v) containing 1 µM chlorpropamide as the internal standard. SP:SAMPLEPREP_SUMMARY The samples were vortexed briefly followed by thorough homogenization. The SP:SAMPLEPREP_SUMMARY samples were then snap frozen with liquid nitrogen and immediately thawed at SP:SAMPLEPREP_SUMMARY room temperature. This step was repeated for three times followed by SP:SAMPLEPREP_SUMMARY centrifuging for 10 min at 12,000 x g and 4 °C. The supernatants were SP:SAMPLEPREP_SUMMARY transferred into fresh microfuge tubes. The remaining cell pellets were SP:SAMPLEPREP_SUMMARY re-extracted with 0.5 ml 50% methanol containing 1 µM chlorpropamide, SP:SAMPLEPREP_SUMMARY homogenized, frozen and thawed three times, spun down, and the supernatants were SP:SAMPLEPREP_SUMMARY combined with the first extraction. Metabolites-containing supernatants were SP:SAMPLEPREP_SUMMARY concentrated to dryness at room temperature in a SpeedVac concentrator and SP:SAMPLEPREP_SUMMARY re-dissolved in 100 µl 97:3 water:methanol (v/v). After centrifuging for 10 min SP:SAMPLEPREP_SUMMARY at 13000 × g and 4°C, 70 µl of supernatants were transferred into autosampler SP:SAMPLEPREP_SUMMARY vials for LC-MS analysis. Two types of control were prepared in triplicates to SP:SAMPLEPREP_SUMMARY run in concert with the experimental samples: the process blank control, and the SP:SAMPLEPREP_SUMMARY pooled control containing an equal volume from each experimental sample. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY The sample run order was randomized to reduce bias from instrument drift. 10 µl CH:CHROMATOGRAPHY_SUMMARY sample was subjected to LC-MS analysis on a Exactive Plus Orbitrap mass CH:CHROMATOGRAPHY_SUMMARY spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 UHPLC system CH:CHROMATOGRAPHY_SUMMARY (Thermo Fisher Scientific). Reversed-phase chromatography mode was used to CH:CHROMATOGRAPHY_SUMMARY separate compounds on a Xselect C18 HSS column (Waters) with solvent A (97:3 CH:CHROMATOGRAPHY_SUMMARY water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid ) and solvent B CH:CHROMATOGRAPHY_SUMMARY (methanol). The flow rate was 200 µl/min, and the total run time was 25 min. CH:CHROMATOGRAPHY_SUMMARY The gradient was 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 CH:CHROMATOGRAPHY_SUMMARY min, 95% B; and 21 min, 0% B. The mass spectrometer was operated in a CH:CHROMATOGRAPHY_SUMMARY negative-ion mode at a resolution of 140,000 at m/z 200 and with a scan range of CH:CHROMATOGRAPHY_SUMMARY 85 to 1000 m/z. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Waters XBridge C18 (50 x 2.1mm,3um) CH:SOLVENT_A 97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid CH:SOLVENT_B methanol CH:FLOW_GRADIENT 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and CH:FLOW_GRADIENT 21 min, 0% B. CH:FLOW_RATE 200 µl/min CH:COLUMN_TEMPERATURE standard #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Raw data files were converted to .mzML file format using the ProteoWizard MS:MS_COMMENTS software, and they were analyzed by the MS-DIAL software. Metabolites were MS:MS_COMMENTS identified by comparison to an in-house reference library of pure metabolite MS:MS_COMMENTS standards which included mass-to-charge ratio (m/z) and retention time. For MS:MS_COMMENTS quantification of metabolite abundance, peak areas of identified metabolites MS:MS_COMMENTS were first normalized to the internal standard chlorpropamide, and then MS:MS_COMMENTS normalized to cell numbers from each sample. Data were analyzed using R and MS:MS_COMMENTS Cytoscape software. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak area normalized to IS and cell number MS_METABOLITE_DATA_START Samples 0-1 0-2 0-3 0-4 0-5 1-1 1-2 1-3 1-4 1-5 3-1 3-2 3-3 3-4 3-5 8-1 8-2 8-3 8-4 8-5 Factors Time_point(days):0 Time_point(days):0 Time_point(days):0 Time_point(days):0 Time_point(days):0 Time_point(days):1 Time_point(days):1 Time_point(days):1 Time_point(days):1 Time_point(days):1 Time_point(days):3 Time_point(days):3 Time_point(days):3 Time_point(days):3 Time_point(days):3 Time_point(days):8 Time_point(days):8 Time_point(days):8 Time_point(days):8 Time_point(days):8 Pyruvate 0.0781171 0.075739521 0.046675136 0.097275332 0.084606164 0.062302729 0.057855074 0.128987132 0.105897256 0.118214166 0.139361579 0.106224463 0.212483238 0.172753602 0.169101628 0.061509917 0.058379876 0.061965875 0.05626 0.062609223 Alanine/Sacrosine 0.012485309 0.01210366 0.006861107 0.011709645 0.009232346 0.008831756 0.008429879 0.014645969 0.012577182 0.012603288 0.014132607 0.012891598 0.020813436 0.024461585 0.020701753 0.008388031 0.009381693 0.008361877 0.009353764 0.009686917 Lactate 0.211782637 0.123305682 0.070735976 0.155245569 0.127551598 0.12924365 0.076301274 0.175768786 0.188992056 0.165518808 0.205602027 0.196352587 0.654433468 0.44949005 0.362866556 0.060190714 0.068669251 0.061748743 0.071399842 0.059357043 Acetoacetate 0.000398291 0.000939158 0.000348125 0.00260656 0.001979894 0.001367456 0.000977968 0.002957484 0.001143959 0.000755357 0.002543577 0.002884899 0.004476345 0.004377769 0.005157684 0.0014481 0.003625545 0.001305469 0.000747832 0.000420465 Serine 0.013376953 0.014662874 0.007708712 0.011319545 0.009542208 0.00888324 0.009774786 0.016168816 0.010104873 0.011111259 0.008032665 0.007282316 0.00761375 0.018023169 0.012397951 0.011341929 0.013019827 0.013662875 0.012995656 0.014883841 Proline 0.016654917 0.018585092 0.012729826 0.015455517 0.013088752 0.017269776 0.018838689 0.026094036 0.019595104 0.01751889 0.036943411 0.029679924 0.032227354 0.045487683 0.040046294 0.016914265 0.016133803 0.016369431 0.016104697 0.020073989 Fumarate 0.000442709 0.000121302 2.11E-05 0.000174764 0.000296768 0.00010395 2.85E-05 0.000302824 0.000307349 0.000185107 0.000252976 0.000310575 0.000713779 0.000995933 0.000226204 0.000189402 3.47E-05 0.000212496 3.73E-05 4.31E-05 Maleic acid 0.002082825 0.001787896 0.001108215 0.002039504 0.001771435 0.001480469 0.001711728 0.003177961 0.002276133 0.002404137 0.002995351 0.002323816 0.005157503 0.004959094 0.004490396 0.001999357 0.002403529 0.002444888 0.001819957 0.001989318 2-Keto-isovalerate 0.009509354 0.006450283 0.004144369 0.009982963 0.008740025 0.005968911 0.005076737 0.012067584 0.010657005 0.012911477 0.012019545 0.008294359 0.02339836 0.021806479 0.019338392 0.005329646 0.00592242 0.005416387 0.006025286 0.006299001 Acetyl-glycine 0.001279079 0.000993846 0.000471437 0.001366203 0.001101248 0.000933475 0.00076676 0.001916686 0.001418265 0.001456153 0.001962314 0.001293296 0.00307906 0.00278266 0.00229977 0.000761443 0.000672658 0.000773359 0.00067695 0.000787783 Guanidoacetic acid 0.000110796 0.000110432 5.00E-05 0.000110882 6.59E-05 7.14E-05 6.90E-05 7.33E-05 0.000111001 6.98E-05 0.000315519 0.000243569 0.000225129 0.000393122 0.000333437 0.000194608 0.00024613 0.00021141 0.000185964 0.000211419 Valine/5-Aminopentanoic acid 0.039913119 0.045499895 0.029248482 0.031470158 0.023384278 0.032469975 0.036073852 0.04551819 0.033690759 0.033081047 0.029906445 0.032413568 0.055236628 0.048020931 0.039902136 0.039015531 0.046820506 0.046614323 0.039019836 0.045935015 Succinate/Methylmalonic acid 0.028492307 0.021796845 0.009635998 0.027541692 0.023609931 0.012428611 0.009964963 0.02557427 0.027328431 0.0297116 0.034376076 0.029438831 0.104059163 0.069517115 0.064344033 0.011788057 0.01093378 0.010711969 0.010045282 0.011192576 Threonine/Homoserine 0.014233636 0.016239846 0.007194168 0.010629798 0.007718493 0.008725341 0.008244871 0.013241463 0.011343083 0.011262074 0.007338317 0.008600034 0.008739723 0.015324586 0.012071326 0.013405687 0.01333038 0.011189739 0.013357647 0.015441173 Taurine 0.61649202 0.621985902 0.449779514 0.752406555 0.688535157 0.515483908 0.532270805 1.049629029 0.709339113 0.862092194 1.042753534 0.744840847 0.567970971 1.325419316 1.082974991 0.56793573 0.593780348 0.658131484 0.559063538 0.5671591 Pyroglutamic acid 0.086016169 0.071707667 0.03886372 0.080515133 0.072084951 0.066489968 0.044853502 0.104274837 0.10507227 0.086109818 0.120759956 0.122595679 0.313545203 0.276288987 0.190372597 0.041716958 0.050599001 0.052772456 0.051767407 0.042256979 Pipecolic Acid 0.000807961 0.000690363 0.000464873 0.001287578 0.00079134 0.000420054 0.000487351 0.001401091 0.001010409 0.001182426 0.00196577 0.001378584 0.002579925 0.002882168 0.00227153 0.001219511 0.001617631 0.001448195 0.001096122 0.000984564 Citraconic acid 0.022695367 0.016376192 0.007775876 0.024046204 0.020299507 0.013088657 0.011723527 0.027000314 0.020868773 0.02587875 0.027852431 0.01975952 0.04519249 0.049183111 0.048836411 0.012257814 0.011211791 0.013439404 0.011944076 0.013306847 Ketoleucine 0.03387256 0.024681539 0.011777947 0.030197028 0.029855659 0.017242876 0.016305466 0.037401428 0.031062465 0.04020742 0.033822039 0.029068788 0.075751923 0.072753654 0.070569459 0.019621888 0.017338516 0.020879447 0.018138626 0.021268111 Hydroxyproline/Aminolevulinate 0.00357595 0.002241184 0.001248195 0.003917994 0.002794687 0.002064801 0.001068154 0.003724185 0.003495545 0.004445668 0.004704322 0.00447986 0.010197389 0.010769538 0.009427397 0.00620041 0.007357091 0.00493885 0.005151881 0.004992421 N-Acetyl-L-alanine 0.003244507 0.002905019 0.002258147 0.003143837 0.00266839 0.00375366 0.00539194 0.006127209 0.004419733 0.004079686 0.004858688 0.004044481 0.006357966 0.00704626 0.004917599 0.006331235 0.010390757 0.010804923 0.007646946 0.008358396 Isoleucine 0.055631937 0.063826774 0.03713431 0.043585546 0.032332681 0.03547847 0.046133042 0.056861698 0.044337116 0.040980585 0.041516849 0.045426967 0.07831327 0.068260473 0.055626067 0.06258029 0.084068317 0.078002068 0.063988526 0.071230655 Leucine 0.132299284 0.166133286 0.112759497 0.116754553 0.090043636 0.088705877 0.135664001 0.152527605 0.105938295 0.10891551 0.111486586 0.10828545 0.155753778 0.156693876 0.134602615 0.167781281 0.211460772 0.213186752 0.1532262 0.201371344 Asparagine 0.008452362 0.010129885 0.005593884 0.006628048 0.005190895 0.008682589 0.00883218 0.013448069 0.007861722 0.009226158 0.004681184 0.004522285 0.00294792 0.005763959 0.005328411 0.008056122 0.009758384 0.010321872 0.009464333 0.011216391 Hydroxyisocaproic acid 0.01587782 0.015271536 0.004650575 0.019184916 0.014695882 0.007810336 0.007655425 0.022032005 0.011684803 0.029455054 0.015869507 0.013749126 0.030080817 0.036969707 0.038030303 0.007389772 0.007279156 0.010073181 0.007953877 0.011001321 Ornithine 0.001286328 0.000857675 0.000317417 0.000848555 0.000524005 0.000431519 0.000470123 0.000685736 0.000831867 0.00091551 0.00079159 0.001081776 0.002807553 0.002628615 0.002103789 0.000319065 0.000244214 0.000697329 0.000331139 0.000333324 Aspartate 0.214595622 0.251037758 0.232054017 0.210216316 0.189892764 0.262554648 0.33131591 0.48109306 0.336839799 0.332488953 0.568599249 0.41709223 0.469306482 0.604486555 0.438601576 0.373137898 0.286530929 0.337219316 0.31078245 0.435277995 Malate 0.003685943 0.00367383 0.001727121 0.004397477 0.003325613 0.002054439 0.00287568 0.004870601 0.003357188 0.005897993 0.005512172 0.00406214 0.005525682 0.006125538 0.007129301 0.001840764 0.001903503 0.002325638 0.001988695 0.00243675 p-Hydroxybenzoate 0.000465606 0.000269838 0.00020162 0.000390931 0.000682316 0.000258214 0.00021489 0.000513986 0.00038408 0.000616801 0.000612229 0.000411676 0.001085771 0.000826723 0.000932609 0.000220972 0.000222128 0.000230675 0.000306562 0.000189881 Alpha-ketoglutarate 0.002146895 0.00161345 0.00082354 0.002135506 0.001893954 0.001188503 0.001279054 0.002596771 0.0020029 0.002407964 0.002691712 0.002425649 0.004813979 0.005336733 0.004413657 0.001609684 0.00160549 0.001783552 0.001431943 0.001527606 Glutamine 0.026928109 0.028858621 0.011577001 0.02079176 0.013365986 0.014470238 0.010273136 0.018087268 0.016232591 0.015681085 0.00765171 0.008455044 0.019359647 0.017554387 0.017227812 0.014648213 0.016604804 0.014476151 0.016260268 0.019730966 Lysine 0.002257611 0.003183836 0.001869206 0.001415642 0.001202184 0.002000738 0.002525845 0.003209192 0.001693904 0.001768747 0.001675078 0.00127081 0.000767399 0.001261181 0.000940474 0.001482771 0.001664656 0.002104025 0.001643385 0.002021943 O-Acetyl-serine 0.252985459 0.256874151 0.206211253 0.208798436 0.214070243 0.306804342 0.330979683 0.439427702 0.377941368 0.301356209 0.488915295 0.434266991 0.634241766 0.63557122 0.515871214 0.404818819 0.417858201 0.425572032 0.356295079 0.429896402 2-Oxo-4-methylthiobutanoate 3.59E-05 2.38E-05 2.28E-05 4.04E-05 5.03E-05 2.55E-05 3.73E-05 3.05E-05 5.00E-05 3.39E-05 0.000676704 0.000333406 0.000228479 0.000398988 0.000287827 6.59E-05 7.71E-05 6.69E-05 6.65E-05 4.97E-05 Methionine 0.017721507 0.026538256 0.022199272 0.017041489 0.012829523 0.012529409 0.021981213 0.020077606 0.012056115 0.011413564 0.014657958 0.015400581 0.010559262 0.014709352 0.013100058 0.041912764 0.050504378 0.050251276 0.039147956 0.048249127 Ribose 0.001225154 0.000774644 0.000345468 0.001203727 0.00081423 0.000629958 0.000422476 0.001006085 0.001190796 0.00126832 0.001204704 0.001201945 0.005389797 0.002886042 0.002814692 0.00034585 0.000349054 0.00036701 0.000387762 0.000399143 Xanthine 0.017152886 0.020737515 0.01415367 0.014711639 0.012218893 0.025771183 0.046776761 0.039106327 0.023712724 0.017013493 0.017142607 0.01821825 0.036745113 0.023652473 0.022419688 0.037461674 0.043116658 0.04813474 0.038453289 0.037574146 2,3-Dihydroxybenzoic acid 0.000814866 0.000576732 0.00024999 0.000828652 0.000778096 0.000337833 0.000421374 0.000879769 0.000677356 0.001041778 0.001062539 0.000683923 0.001542334 0.001802 0.001739954 0.000449652 0.000364739 0.000521948 0.000399706 0.000474609 Histidine 0.001191114 0.002209476 0.001527614 0.000924479 0.000767937 0.001653008 0.001808141 0.002350756 0.001339694 0.001129915 0.000765125 0.0010418 0.000867069 0.000678007 0.000563498 0.001046917 0.001309292 0.001309674 0.001171829 0.001304591 Allantoin 0.009083871 0.00671485 0.002991702 0.009254733 0.00543953 0.004495691 0.002984027 0.007143391 0.00928756 0.008888799 0.011185857 0.010947734 0.035217717 0.024016137 0.022153725 0.002660719 0.002649441 0.001597865 0.002208829 0.002313653 Phenylpyruvate 0.004440888 0.003074996 0.001643663 0.004798105 0.004186554 0.002398415 0.002205888 0.005144427 0.004182119 0.00531555 0.005613235 0.004115827 0.010265114 0.010197957 0.009474103 0.00253883 0.002464207 0.002560687 0.002331333 0.002654585 Phenyllactic acid 0.003906933 0.002607829 0.00124618 0.004120329 0.003459293 0.001991435 0.001939571 0.004451539 0.003417545 0.00439993 0.004529692 0.003525579 0.008437496 0.008662189 0.008013304 0.001972447 0.001963772 0.002205517 0.001820165 0.002127146 Quinolinate 0.000435146 0.000516584 0.000507464 0.000374423 0.000332864 0.000317558 0.000367116 0.000620509 0.000518426 0.000393253 0.00022444 0.00027833 0.000465881 0.000348572 0.000411027 0.000371626 0.000249409 0.000393151 0.000208231 0.000300378 Phosphoenolpyruvate 0.002753557 0.004061558 0.004110271 0.003088534 0.00243074 0.006179658 0.001988646 0.003051471 0.004934648 0.002816906 0.010425669 0.009499828 0.007626577 0.013049292 0.007503014 0.003563671 0.004173318 0.001565568 0.003128046 0.002995238 Glyceraldehdye-3-phosphate 0.003079501 0.006897212 0.010583538 0.003932024 0.00763369 0.010051116 0.027210345 0.031138358 0.006350324 0.004221541 0.008370306 0.004789336 0.000992171 0.004193473 0.004404238 0.01243674 0.015103384 0.025595894 0.016132971 0.017366406 sn-Glycerol-3-phosphate 0.006024237 0.005496971 0.003402237 0.003199818 0.003231326 0.006964511 0.004759892 0.006613712 0.005129202 0.004405651 0.015107764 0.012760009 0.010811032 0.018930611 0.015071368 0.014276843 0.012270786 0.012972054 0.012546965 0.017037523 Aconitate 0.059137404 0.042250425 0.02151066 0.05986455 0.050347216 0.033546932 0.027768749 0.065126544 0.060316537 0.071652134 0.082373518 0.06406923 0.15490981 0.142639944 0.13126698 0.033294713 0.029995671 0.03201512 0.030003405 0.031456281 N-Acetyl-L-ornithine 0.001845615 0.0014713 0.000929062 0.001574339 0.001223226 0.001367404 0.001273525 0.002065435 0.001905845 0.002011041 0.001917284 0.001897317 0.005379632 0.003710445 0.003241812 0.000632856 0.00061502 0.000830045 0.000616017 0.000684835 Arginine 0.00220843 0.00310454 0.001630506 0.001544789 0.001385472 0.002052898 0.002720953 0.003366259 0.001727932 0.00188401 0.00165452 0.001173144 0.00082987 0.001159585 0.001062226 0.001961841 0.002700308 0.003296769 0.00244897 0.002800155 Citrulline 0.002045452 0.001606101 0.00058036 0.001706284 0.001068169 0.000859296 0.000554835 0.00135219 0.001743149 0.00177963 0.001498096 0.001714278 0.005794796 0.00539267 0.003832619 0.000443674 0.000523779 0.000516843 0.000680795 0.000573525 Allantoate 0.000799423 0.000565966 0.00025742 0.000668776 0.000496707 0.000308768 0.000226038 1.66E-05 0.000664848 0.000626411 0.000865653 0.000903064 5.37E-07 0.001705524 0.001580964 0.00010925 9.52E-05 1.23E-06 8.04E-05 9.08E-05 2-Isopropylmalic acid 0.019724222 0.013560774 0.007130688 0.022496903 0.018308128 0.010639717 0.009399561 0.022896495 0.018721358 0.024622736 0.024592436 0.017073786 0.042082979 0.041959567 0.042937371 0.01156281 0.010835645 0.011702937 0.011321528 0.010757588 Glucono-_-lactone 0.000191481 8.78E-05 0.000111593 0.000252541 2.76E-05 0.000236525 4.45E-05 6.83E-05 0.000211304 0.000121031 0.000185578 0.000130937 0.000361759 0.000636451 0.000324371 8.30E-05 0.000118498 7.05E-05 0.000115931 8.55E-05 Glucosamine 0.000334258 0.000133551 6.04E-05 0.000319361 0.000205163 0.000143289 0.000174734 0.000338002 0.000278957 0.000293924 0.000374678 0.000203119 0.000370979 0.000606432 0.000731213 0.000147137 9.36E-05 0.000136379 0.000112277 0.000126363 Hydroxyphenylpyruvate 0.000953429 0.000825484 0.000332337 0.00144617 0.001152762 0.000535804 0.00060962 0.001426255 0.001143083 0.001408606 0.001413762 0.001021832 0.002210301 0.002118776 0.002214945 0.000745092 0.000623705 0.000805757 0.000532693 0.000776742 Glucose 0.038404401 0.024281479 0.009235564 0.036482052 0.024693831 0.014510507 0.007760901 0.023290185 0.036802749 0.035651366 0.034616285 0.039454725 0.192652114 0.097648531 0.095488088 0.003915542 0.003308373 0.002694717 0.003633989 0.003520497 Tyrosine 0.02874109 0.03426619 0.023681423 0.026281923 0.022106031 0.021769994 0.040001303 0.041576015 0.025395762 0.028656176 0.026331293 0.024780244 0.039582168 0.036955019 0.032698916 0.051135719 0.067439821 0.086880748 0.052332984 0.065387284 4-Pyridoxic acid 0.001832096 0.0012841 0.000594922 0.001919037 0.001619315 0.000880736 0.000860218 0.002251452 0.001697321 0.002312368 0.00199518 0.001558402 0.004129185 0.004145973 0.003869194 0.00099963 0.000931698 0.001049838 0.00094008 0.00109534 3-Phosphoglycerate 0.011741808 0.012058284 0.005371328 0.0093105 0.00854869 0.008884129 0.010454398 0.013477308 0.014932569 0.012549298 0.027918834 0.019515856 0.016729781 0.032244292 0.027734989 0.012279272 0.011067904 0.009604851 0.012008794 0.014903069 Acetyllysine 0.000981321 0.001544939 0.000554015 0.000623777 0.000493678 0.00033377 0.000424753 0.000437313 0.0005767 0.000321536 0.000518567 0.000539687 0.000746103 0.000659708 0.000620759 0.000515331 0.000548197 0.000447513 0.00056393 0.000670375 Citrate/isocitrate 0.183385277 0.08523824 0.10549351 0.287311994 0.20902333 0.157371837 0.177511449 0.365037017 0.322130306 0.364021063 0.484285759 0.359922205 0.850894568 0.80644741 0.728244604 0.215358979 0.17618693 0.218448007 0.174652014 0.199599245 2-Keto-D-gluconate 0.001193454 0.00105319 0.000693165 0.001038673 0.000862787 0.000905567 0.001552736 0.001694105 0.001598778 0.0015058 0.001797618 0.001686553 0.004395727 0.002782952 0.002575938 0.003295755 0.005824638 0.0049134 0.003322701 0.003733553 Gluconate 0.004693579 0.004272927 0.003396481 0.004363078 0.010708483 0.003422406 0.004823247 0.011678369 0.005800794 0.005151663 0.010422065 0.008084816 0.015479604 0.014050158 0.013236438 0.009208165 0.013966973 0.009936425 0.007099167 0.00890491 Tryptophan 0.004067524 0.005622898 0.00492794 0.00403086 0.003741672 0.007823614 0.007927229 0.010172569 0.005889611 0.005691455 0.005382282 0.004826584 0.004173324 0.005937546 0.004535143 0.006892931 0.009723111 0.01146156 0.007891641 0.009066961 Deoxyribose-phosphate 0.001225852 0.003117456 0.000729817 0.00071719 0.000365113 0.000876432 0.000856317 0.000616299 0.002250215 0.000510239 0.001310356 0.001114903 0.000617261 0.000830415 0.000872276 0.001163704 0.000791424 0.000543712 0.001038988 0.001358128 Pantothenate 0.011853199 0.008926182 0.005428792 0.011387733 0.007905264 0.00762206 0.00766262 0.014340137 0.011653446 0.013139664 0.015316535 0.013033735 0.032775891 0.027733663 0.027723875 0.006275611 0.0063502 0.007835803 0.006168447 0.008316657 Cystathionine 0.000112002 9.22E-05 6.46E-05 0.000126865 0.000118918 0.000550097 0.000468474 0.000942734 0.000409405 0.000582407 0.000679163 0.000295028 0.000235586 0.000583397 0.00063505 7.85E-05 0.000112883 0.000157918 8.93E-05 8.10E-05 Ribulose-5-phosphate 0.010000004 0.013302268 0.007962862 0.011850333 0.007293935 0.001275405 0.000720081 0.001169724 0.001963339 0.005188864 0.003823393 0.003195234 0.002093939 0.003674225 0.004087965 0.007195382 0.001743593 0.001248953 0.002588216 0.002588763 Ribose-5-phosphate 0.008562629 0.009507671 0.010264773 0.007336 0.009936031 0.014018104 0.007122134 0.014316925 0.015239072 0.010446114 0.007937999 0.005984198 0.003273804 0.006995432 0.006057175 0.00243798 0.005847735 0.006155084 0.009219396 0.006827192 Tetrahydrobiopterin 0.000336242 0.000208681 6.89E-05 0.000246919 0.000147143 0.000117371 9.33E-05 0.00015129 0.000300834 0.00028982 0.000205206 0.000254776 0.000355964 0.000543399 0.000543463 0.000111962 8.73E-05 0.000109071 0.000153785 0.000147114 Cytidine 0.028954003 0.029293841 0.020934381 0.031287262 0.02511201 0.022566602 0.02700016 0.045596615 0.031861448 0.035192273 0.029164383 0.021925514 0.022958571 0.040508238 0.033703099 0.030978546 0.031892237 0.026589336 0.026997728 0.030292963 Uridine 0.001929187 0.001377272 0.000727226 0.001604561 0.001139756 0.001031979 0.000922741 0.001436714 0.001882447 0.001769161 0.002441427 0.002451945 0.007906279 0.004788915 0.00448029 0.000618423 0.000605264 0.000610862 0.000550307 0.000599395 Leu-Asp (LD) 0.000309508 0.000319992 0.000159419 0.000320161 0.000307898 0.000312239 0.000431103 0.00072865 0.000426156 0.000587133 0.000370439 0.000470115 1.15E-05 0.000738594 0.000549913 0.000301707 0.000360833 0.000399728 0.000365415 0.000426564 Glucose-1/6-phosphate@6.5 0.010381329 0.011359538 0.011299709 0.008972948 0.008136115 0.019651732 0.03126288 0.027845295 0.017215187 0.013124937 0.014460211 0.011284328 0.001628234 0.015692252 0.01226405 0.017865467 0.024552768 0.026791209 0.025084036 0.02419534 Glucose-1/6-phosphate@7.23 0.00910905 0.010419642 0.01027904 0.008910161 0.007634675 0.016811414 0.030320653 0.02535149 0.013325131 0.011628739 0.011333289 0.008858417 0.004744324 0.012571786 0.010433929 0.013035948 0.021991838 0.026981859 0.02252333 0.020192842 Fructose-6-phosphate 0.00028355 0.000580093 0.000610688 0.000427991 0.000469875 0.000473367 0.000752421 0.00094749 0.000466885 0.000407196 0.000588407 8.06E-05 0.000278223 0.00048078 0.000415586 0.000493667 0.00062325 0.000669063 0.000579589 0.000653214 6-Phospho-D-gluconate 0.00117814 0.001218309 0.000955868 0.000931168 0.000727541 0.000955992 0.001456342 0.002014513 0.001184432 0.001091817 0.00118379 0.000994605 0.001368099 0.001273665 0.001214597 0.000604295 0.000604286 0.000433808 0.000492995 0.000582192 Xanthosine 8.87E-05 9.75E-05 5.22E-05 4.35E-05 3.97E-05 0.000119307 0.0002474 0.000119273 0.000107748 9.17E-05 0.000571489 0.000887176 0.000461095 0.000517042 0.000499408 0.000343581 0.000427148 0.00056521 0.000389986 0.000310059 D-Sedoheptulose-1/7-phosphate 0.001550977 0.001641951 0.002399554 0.001221339 0.001436862 0.007141929 0.011298854 0.013493316 0.004556919 0.00200915 0.003052126 0.001524482 0.000658777 0.002142176 0.001680335 0.007260161 0.012057794 0.018311096 0.015075003 0.013368579 N-Acetyl-glucosamine-1/6-phosphate 0.000598578 0.000574221 0.000452952 0.000411524 0.000156104 0.000311085 0.000638849 0.001034884 0.00098468 0.000583139 0.001012092 0.000857437 0.000660751 0.001172426 0.000859243 0.00095184 0.001345939 0.001522659 0.000779593 0.001042378 dCMP 0.002913695 0.001524209 0.001923361 0.001277256 0.001278764 0.002800336 0.005389008 0.00272066 0.004990978 0.000876433 0.000873012 0.000891992 0.000359985 0.000466724 0.000501224 0.00112053 0.001324089 0.001898719 0.000927261 0.001042982 Glutathione 0.039530204 0.055626777 0.069180503 0.053018598 0.022149744 0.129642015 0.128309025 0.116756775 0.12969898 0.052025922 0.271154771 0.237753004 0.170446748 0.196039447 0.186922312 0.230910542 0.269737355 0.240555013 0.211013884 0.223457606 dTMP 0.008535626 0.004341986 0.005912127 0.004215913 0.004047543 0.007244864 0.01526725 0.008052051 0.012690977 0.003038794 0.00181443 0.001739394 0.000919669 0.001033945 0.0010345 0.002917641 0.003850956 0.005725643 0.00237408 0.001839369 UMP 0.010422331 0.010972071 0.008552376 0.011435783 0.010663989 0.010444103 0.020400591 0.011947331 0.017479462 0.007128433 0.00328914 0.003343705 0.002642013 0.004064445 0.002864198 0.009635685 0.008404329 0.007452751 0.00524389 0.0084098 Cyclic-AMP 0.001266709 0.001062498 0.001066343 0.001489287 0.001082525 0.000976006 0.00072201 0.000942175 0.001537533 0.001159646 0.002029506 0.001531726 0.00357028 0.003302325 0.002450509 0.000947613 0.001064483 0.000551593 0.000765327 0.000739916 dAMP 0.00733806 0.003444217 0.005029267 0.003414697 0.003231887 0.006418325 0.01402208 0.006849374 0.010823067 0.002589103 0.001523678 0.001316028 0.000804543 0.000914782 0.000844547 0.002172727 0.003150966 0.004945675 0.001945316 0.001657916 Trehalose/Sucrose 0.001223516 0.000641351 0.000336104 0.001915901 0.007015768 0.000870847 0.000446416 0.008414264 0.002548891 0.002170383 0.002326137 0.00090177 0.00285075 0.003333907 0.003681829 0.000677548 0.000534426 0.003242093 0.000636655 0.000617242 dGMP 0.11039561 0.121475111 0.123242282 0.100223 0.070974706 0.119240775 0.144431365 0.189602947 0.163688925 0.13256624 0.260927536 0.216685074 0.093059925 0.239563885 0.200927231 0.200601535 0.204363528 0.190602652 0.159993815 0.18634498 AMP 0.108629203 0.119636944 0.120579969 0.098563349 0.070004467 0.116956657 0.141192433 0.185952358 0.160424684 0.131100643 0.256919835 0.212347703 0.09151624 0.235602848 0.197408549 0.197974622 0.200339352 0.187828754 0.157035303 0.183076925 GMP 0.036235572 0.027742575 0.019079533 0.028946497 0.026946663 0.022704079 0.042363505 0.024903615 0.04810543 0.017506256 0.007707795 0.008563916 0.003731327 0.00803106 0.006079141 0.014855232 0.012518427 0.007771805 0.006271554 0.011637762 dCDP 3.16E-06 7.29E-05 5.20E-05 2.15E-05 8.82E-06 0.000117709 5.71E-05 0.000102316 0.000224663 0.000146391 0.000439499 0.000450223 0.000106248 0.000386313 0.000224941 7.79E-05 8.48E-05 1.54E-05 3.18E-05 3.09E-05 5-Phosphoribosyl-1-pyrophosphate 8.33E-05 0.002414921 7.25E-06 0.003656791 0.002004107 0.000351746 0.000711014 0.002862547 0.000528499 0.001055553 0.004146942 0.000991434 0.000222083 0.005359543 0.008921472 0.002450781 0.000140433 0.000152898 0.000989489 0.001934748 dTDP 2.07E-05 1.32E-05 5.07E-05 1.98E-05 1.09E-05 3.14E-05 6.74E-06 4.05E-05 7.42E-05 4.28E-05 0.000225549 8.35E-05 0.000646104 0.000106486 8.72E-05 2.71E-05 0.000130221 1.07E-05 1.20E-05 1.34E-05 CDP 0.001603925 0.000943358 0.000466401 0.001568477 0.000883914 0.000982627 0.000220969 0.000703352 0.004328925 0.001746214 0.010114232 0.00949887 0.011443289 0.017858102 0.012569976 0.000285492 0.000382461 0.00024989 0.000280833 0.000130815 UDP 0.028234415 0.015621474 0.032389064 0.045759296 0.019029644 0.060974466 0.011693794 0.044650017 0.084760662 0.042114276 0.075005303 0.090743146 0.254690888 0.179409342 0.100101445 0.022195384 0.04085136 0.015170275 0.025073409 0.010946783 Cholic acid 0.001548161 0.001072667 0.000562619 0.001446527 0.00133598 0.000871273 0.000747532 0.001761007 0.001503661 0.001627444 0.001621859 0.001210327 0.003692836 0.003438447 0.00335771 0.00085306 0.000723217 0.00088085 0.000718084 0.000836925 ADP 0.018312454 0.020875807 0.029836311 0.040132893 0.02285838 0.054341515 0.011242282 0.046771181 0.056958534 0.027499316 0.042515376 0.045189102 0.220322588 0.100123833 0.070640382 0.010780886 0.020031287 0.010162392 0.026514284 0.016100035 dCTP 1.02E-06 7.28E-07 0.000317058 0.000377883 9.14E-07 0.000181997 4.82E-07 0.001218267 0.000503315 1.24E-06 1.22E-05 0.001157877 0.001422163 0.002435582 2.18E-06 0.000158556 0.000728183 5.93E-07 0.000323641 5.90E-07 dTTP 0.001531997 0.000593518 0.000874402 0.000805866 0.000818047 0.002489425 0.000586578 0.000832836 0.00250591 0.001626482 0.001557789 0.002081546 0.004590847 0.001786906 0.002294341 0.000515028 0.001078428 0.000942858 0.000679451 0.000529894 CTP 0.000183952 0.002718142 0.008088218 0.063298524 0.000651976 0.017606272 2.87E-05 0.068910159 0.038185626 5.04E-05 0.043538014 0.033162966 0.058489529 0.114925024 0.048314915 0.03030042 0.017933262 0.000112368 0.021809401 0.006580228 UTP 0.123139014 0.040210472 0.141470452 0.192095522 0.060667735 0.272387934 0.043609611 0.21364834 0.285869716 0.131383313 0.118576144 0.198753849 0.984614672 0.408601815 0.146020381 0.096589948 0.19004683 0.0712276 0.101050942 0.031981937 dATP 7.59E-05 0.000326753 0.000924673 0.000708321 0.000447426 0.001462898 0.00023773 0.001042661 0.001814281 0.000303056 0.000808027 0.001204776 0.004272481 0.000974117 0.001349691 0.000342406 0.000811459 2.80E-05 0.000622639 0.000212292 dGTP 0.251245599 0.258999569 0.298618481 0.432791599 0.303215085 0.501936531 0.14479047 0.529339762 0.5525403 0.350354391 0.439243131 0.496192011 1.846218972 1.000414007 0.773224694 0.219196982 0.326485996 0.15964392 0.281781423 0.225226926 GTP 0.021124793 0.01812637 0.029608477 0.041709771 0.023952794 0.005561906 0.011856467 0.060175927 0.083106015 0.02919892 0.03288129 0.070430445 0.264331597 0.126920118 0.052607543 0.020807017 0.047610573 0.012386978 0.036584547 0.015596609 UDP-D-glucose 0.003500579 0.00363961 0.004189746 0.002858516 0.002586645 0.003314757 0.003696069 0.005457832 0.004528727 0.003758025 0.003083819 0.003336111 0.003341603 0.003831743 0.003110293 0.006491581 0.005810199 0.007260186 0.005747589 0.006275121 UDP-N-acetyl-glucosamine 0.008654745 0.009686665 0.010874096 0.007676622 0.007342502 0.012682941 0.014583847 0.017226384 0.013928729 0.011176573 0.030692775 0.022934295 0.022910572 0.032749094 0.023945619 0.029875069 0.031006151 0.026108558 0.026731548 0.02854711 Glutathione disulfide 0.029149592 0.037914028 0.033325527 0.022843623 0.013659545 0.030812695 0.040381918 0.05457267 0.035305662 0.036659382 0.05754401 0.037019086 0.038103777 0.049054527 0.034314952 0.014974529 0.011283228 0.01882797 0.011615841 0.020180015 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Pyruvate Alanine/Sacrosine Lactate Acetoacetate Serine Proline Fumarate Maleic acid 2-Keto-isovalerate Acetyl-glycine Guanidoacetic acid Valine/5-Aminopentanoic acid Succinate/Methylmalonic acid Threonine/Homoserine Taurine Pyroglutamic acid Pipecolic Acid Citraconic acid Ketoleucine Hydroxyproline/Aminolevulinate N-Acetyl-L-alanine Isoleucine Leucine Asparagine Hydroxyisocaproic acid Ornithine Aspartate Malate p-Hydroxybenzoate Alpha-ketoglutarate Glutamine Lysine O-Acetyl-serine 2-Oxo-4-methylthiobutanoate Methionine Ribose Xanthine 2,3-Dihydroxybenzoic acid Histidine Allantoin Phenylpyruvate Phenyllactic acid Quinolinate Phosphoenolpyruvate Glyceraldehdye-3-phosphate sn-Glycerol-3-phosphate Aconitate N-Acetyl-L-ornithine Arginine Citrulline Allantoate 2-Isopropylmalic acid Glucono-_-lactone Glucosamine Hydroxyphenylpyruvate Glucose Tyrosine 4-Pyridoxic acid 3-Phosphoglycerate Acetyllysine Citrate/isocitrate 2-Keto-D-gluconate Gluconate Tryptophan Deoxyribose-phosphate Pantothenate Cystathionine Ribulose-5-phosphate Ribose-5-phosphate Tetrahydrobiopterin Cytidine Uridine Leu-Asp (LD) Glucose-1/6-phosphate@6.5 Glucose-1/6-phosphate@7.23 Fructose-6-phosphate 6-Phospho-D-gluconate Xanthosine D-Sedoheptulose-1/7-phosphate N-Acetyl-glucosamine-1/6-phosphate dCMP Glutathione dTMP UMP Cyclic-AMP dAMP Trehalose/Sucrose dGMP AMP GMP dCDP 5-Phosphoribosyl-1-pyrophosphate dTDP CDP UDP Cholic acid ADP dCTP dTTP CTP UTP dATP dGTP GTP UDP-D-glucose UDP-N-acetyl-glucosamine Glutathione disulfide METABOLITES_END #END