#METABOLOMICS WORKBENCH DATATRACK_ID:3901 STUDY_ID:ST002591 ANALYSIS_ID:AN004263 VERSION 1 CREATED_ON 04-27-2023 #PROJECT PR:PROJECT_TITLE MoTrPAC PR:PROJECT_SUMMARY MoTrPAC is a national research consortium designed to discover and perform PR:PROJECT_SUMMARY preliminary characterization of the range of molecular transducers (the PR:PROJECT_SUMMARY "molecular map") that underlie the effects of physical activity in humans. The PR:PROJECT_SUMMARY program's goal is to study the molecular changes that occur during and after PR:PROJECT_SUMMARY exercise and ultimately to advance the understanding of how physical activity PR:PROJECT_SUMMARY improves and preserves health. Preclinical and clinical studies will examine the PR:PROJECT_SUMMARY systemic effects of endurance and resistance exercise across a range of ages and PR:PROJECT_SUMMARY fitness levels by molecular probing of multiple tissues before and after acute PR:PROJECT_SUMMARY and chronic exercise. This program is the largest targeted NIH investment of PR:PROJECT_SUMMARY funds into the mechanisms of how physical activity improves health and prevents PR:PROJECT_SUMMARY disease. The MoTrPAC program is supported by the NIH Common Fund and is managed PR:PROJECT_SUMMARY by a trans-agency working group representing multiple NIH institutes and PR:PROJECT_SUMMARY centers, led by the NIH Office of Strategic Coordination, National Institute of PR:PROJECT_SUMMARY Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes PR:PROJECT_SUMMARY and Digestive and Kidney Diseases, National Institute on Aging, and National PR:PROJECT_SUMMARY Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: PR:PROJECT_SUMMARY Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion PR:PROJECT_SUMMARY Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac PR:PROJECT_SUMMARY Bioinformatics / Data Coordination Center. PR:INSTITUTE MoTrPAC PR:LAST_NAME Ashley PR:FIRST_NAME Euan PR:ADDRESS Falk Building CV267, 870 Quarry Road, Stanford, California 94305 PR:EMAIL motrpac-data-deposition@lists.stanford.edu PR:PHONE (650) 725-1846 PR:DOI http://dx.doi.org/10.21228/M8V97D #STUDY ST:STUDY_TITLE MoTrPAC: Endurance exercise training study in young adult rats, Rat Hippocampus ST:STUDY_TITLE Powder - Targeted Tricarboxylic Acid Cycle ST:STUDY_SUMMARY The goal of the endurance exercise training study in young adult rats (internal ST:STUDY_SUMMARY code: PASS1B-06) was to perform exercise training studies in adult (6 month) ST:STUDY_SUMMARY F344 rats, and from these rats collect as many tissues as feasible in order to ST:STUDY_SUMMARY provide high quality samples for detailed analysis by chemical analysis sites. ST:STUDY_SUMMARY Tissues were collected from 10-12 rats sedentary control rats concurrent with ST:STUDY_SUMMARY the collection of the 8-week training groups. The 8-week training group and ST:STUDY_SUMMARY controls were from the same cohort and same age at euthanasia (either 8). For ST:STUDY_SUMMARY the older age group, an additional set of controls (n=5-6) were collected with ST:STUDY_SUMMARY the 1-2 week training group. Rats were either sedentary or underwent an exercise ST:STUDY_SUMMARY training program. Rats were exercised on the rodent treadmill 5 days per week ST:STUDY_SUMMARY using a progressive training protocol designed to exercise the rats at ST:STUDY_SUMMARY approximately 70% of VO2max as outlined in the Table on the next page. Training ST:STUDY_SUMMARY was performed no earlier than 10:00 am and no later than 5:00 pm over 5 ST:STUDY_SUMMARY consecutive days per week. Training was initiated with the treadmill set at 70% ST:STUDY_SUMMARY of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of ST:STUDY_SUMMARY exercise was increased by one minute each day until day 31 of training (start of ST:STUDY_SUMMARY week 7), when a duration of 50 min was reached. Speed and grade of each training ST:STUDY_SUMMARY session increased in larger increments due to treadmill parameters. The highest ST:STUDY_SUMMARY intensity and duration of training began on day 31. This intensity was ST:STUDY_SUMMARY maintained for the final 10 days of the protocol to ensure steady state had been ST:STUDY_SUMMARY achieved. If any rats were unable to perform at least 4 days of training per ST:STUDY_SUMMARY week they were removed from the study and euthanized. It is important to note ST:STUDY_SUMMARY that the starting treadmill speed varied depending on the sex and age of the ST:STUDY_SUMMARY rat. The initial and maximum speeds were based on VO2max measurements obtained ST:STUDY_SUMMARY during the pre-training testing of the compliant rats. Rats assigned to the ST:STUDY_SUMMARY control group followed a schedule similar to the training group. They were ST:STUDY_SUMMARY placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The ST:STUDY_SUMMARY treadmill was set at 0 m/min at an incline that corresponded to the incline ST:STUDY_SUMMARY being used by the training group. ST:INSTITUTE Mayo Clinic ST:DEPARTMENT Internal Medicine ST:LABORATORY Mayo Clinic Metabolomics Resource Core (MCMRC) ST:LAST_NAME Nair ST:FIRST_NAME K. Sreekumaran ST:ADDRESS 200 1st St SW, Rochester, MN 55902 ST:EMAIL nair@mayo.edu ST:PHONE (507) 284-2511 ST:SUBMIT_DATE 2023-04-25 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS 1628FB23-6F66-47D4-8D82-63C87726F0FD 90245015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_28.D.zip SUBJECT_SAMPLE_FACTORS 69FD4884-786D-4F2A-97B1-6174FBD25D60 90248015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_29.D.zip SUBJECT_SAMPLE_FACTORS 07AF7369-F082-4CA7-99D5-D973F4A162C9 90252015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_31.D.zip SUBJECT_SAMPLE_FACTORS F9BEE1FA-E2DB-4BE4-A79C-99D585D6FD17 90265015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_35.D.zip SUBJECT_SAMPLE_FACTORS A77AC89A-1D9E-4516-9DA4-2BE99C8126EF 90266015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_36.D.zip SUBJECT_SAMPLE_FACTORS BC49E4EF-E2FE-4269-BAF0-461F768BD95D 90217015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_18.D.zip SUBJECT_SAMPLE_FACTORS 0559B193-BED3-4170-B045-10A2B6441CB6 90229015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_24.D.zip SUBJECT_SAMPLE_FACTORS 90CFE5A4-76FE-4458-A2B1-A66F0B60118E 90232015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_25.D.zip SUBJECT_SAMPLE_FACTORS F0D83B29-8EFA-4CDE-B1AA-2358AC220ECF 90237015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_26.D.zip SUBJECT_SAMPLE_FACTORS FCE9ABE6-B60B-4934-BAE5-56AF8B11ECEC 90239015210 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_27.D.zip SUBJECT_SAMPLE_FACTORS B73AB139-7210-4498-9CCF-E1DBE6EBA71C 90559015210 Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_34.D.zip SUBJECT_SAMPLE_FACTORS EA5C8932-9325-4DA5-96BB-50CD49CEDD10 90560015210 Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_35.D.zip SUBJECT_SAMPLE_FACTORS 0645F621-878C-4C9B-84A6-58862BAA17D9 90564015210 Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_36.D.zip SUBJECT_SAMPLE_FACTORS 6D680EA2-C62B-4A35-8B6E-2ED6A79CB017 90421015210 Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_24.D.zip SUBJECT_SAMPLE_FACTORS 53F914B0-FD4F-4340-84A6-48341EAB3798 90422015210 Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_25.D.zip SUBJECT_SAMPLE_FACTORS D350D633-8D95-4783-99F2-BB8294D2EC32 90423015210 Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_26.D.zip SUBJECT_SAMPLE_FACTORS 0DBFEB04-1B58-43CF-BAFD-9AA9915B67F6 90426015210 Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_27.D.zip SUBJECT_SAMPLE_FACTORS 10E282EB-1DBA-406E-BD5D-81027083A01D 90430015210 Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_28.D.zip SUBJECT_SAMPLE_FACTORS B137E81D-A9DE-4E3A-8E2B-AD6C22920B7E 90576015210 Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_37.D.zip SUBJECT_SAMPLE_FACTORS DC8B35E8-4AA8-4829-8569-F223D069620C 90578015210 Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_38.D.zip SUBJECT_SAMPLE_FACTORS 8904857C-2073-4BBA-AC7C-1EC239E5BFF7 90581015210 Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_39.D.zip SUBJECT_SAMPLE_FACTORS 4E974304-21EB-42E8-A729-A7FB6B406E64 90439015210 Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_29.D.zip SUBJECT_SAMPLE_FACTORS 6527EC12-E7EB-4F17-BF0A-0AC7CD1819A0 90441015210 Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_30.D.zip SUBJECT_SAMPLE_FACTORS AEB1611A-F29E-43F8-B9F3-A2BC4F9EB504 90444015210 Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_31.D.zip SUBJECT_SAMPLE_FACTORS FDA87A8D-3634-4C42-9A1A-0EA03B58BB01 90449015210 Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_32.D.zip SUBJECT_SAMPLE_FACTORS CDCFA3F1-07ED-4737-A36D-15EFEC325AA5 90450015210 Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_33.D.zip SUBJECT_SAMPLE_FACTORS A9CE3E3D-31A0-4443-8A7F-8FD831D79248 90410015210 Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_20.D.zip SUBJECT_SAMPLE_FACTORS 3D5BC47C-BB3D-45A2-8DB8-D7ADCD51997C 90412015210 Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_21.D.zip SUBJECT_SAMPLE_FACTORS 89D6F615-D17C-4EB9-88EB-A602940FF179 90416015210 Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_22.D.zip SUBJECT_SAMPLE_FACTORS FE012DC0-AF32-4C70-A4D4-F042B8BC4EC6 90420015210 Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=22feb21_23.D.zip SUBJECT_SAMPLE_FACTORS 22D700B9-EDD0-4BC8-8155-EB9C39417F81 90280015210 Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_38.D.zip SUBJECT_SAMPLE_FACTORS 1CA4210C-3CE6-4A5A-996B-673EBDEB3B81 90281015210 Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_39.D.zip SUBJECT_SAMPLE_FACTORS 9F3E1040-D409-421E-B635-2572DC20ED89 90283015210 Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_40.D.zip SUBJECT_SAMPLE_FACTORS 569A9229-04E8-414A-A195-FE9E8F1B66AB 90289015210 Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_41.D.zip SUBJECT_SAMPLE_FACTORS E4271AF1-899A-4A65-9D8D-BF18CEE3F1CF 90292015210 Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=22feb21_19.D.zip SUBJECT_SAMPLE_FACTORS 0D5071F5-D166-4CF1-BE8B-EB4DF6E04C0E 90251015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_30.D.zip SUBJECT_SAMPLE_FACTORS 2479BEE5-2A38-4D09-B15B-B1715F561A9F 90254015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_32.D.zip SUBJECT_SAMPLE_FACTORS 0A4A7935-3AF4-463F-84E3-DCF07671509D 90258015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_33.D.zip SUBJECT_SAMPLE_FACTORS 04C84D7E-FC8C-422F-AE4B-CAFADB63407B 90259015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_34.D.zip SUBJECT_SAMPLE_FACTORS 2B969165-1A52-43AA-8FD9-39AFA62F094F 90267015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder raw_file=19feb21_37.D.zip SUBJECT_SAMPLE_FACTORS 0BD282C7-7832-4581-9089-08A32D557034 90218015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_19.D.zip SUBJECT_SAMPLE_FACTORS 955498F4-70A0-4D50-A2BA-619A8FED8FEF 90222015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_20.D.zip SUBJECT_SAMPLE_FACTORS CFE06932-9314-4BCC-B2D9-73883D5FCDFB 90223015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_21.D.zip SUBJECT_SAMPLE_FACTORS 3719CB62-B551-49BE-94A0-E51DFE13D30D 90225015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_22.D.zip SUBJECT_SAMPLE_FACTORS F65955CE-E49F-48C7-B861-02A2FDF6F9B8 90227015210 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder raw_file=19feb21_23.D.zip #COLLECTION CO:SAMPLE_TYPE Hippocampus #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with SP:SAMPLEPREP_SUMMARY ethoxamine, MTBSTFA #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Analytes were chromatographically separated with 17 minutes GC gradient CH:INSTRUMENT_NAME Agilent Intuvo 9000 GC CH:COLUMN_NAME Agilent HP-5MS (30m x 0.250mm, 0.25um) CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 5977B HES MSD MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:MS_COMMENTS SIM; peak integration, concentrations calculated MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nano moles per vial MS_METABOLITE_DATA_START Samples 90245015210 90248015210 90252015210 90265015210 90266015210 90217015210 90229015210 90232015210 90237015210 90239015210 90559015210 90560015210 90564015210 90421015210 90422015210 90423015210 90426015210 90430015210 90576015210 90578015210 90581015210 90439015210 90441015210 90444015210 90449015210 90450015210 90410015210 90412015210 90416015210 90420015210 90280015210 90281015210 90283015210 90289015210 90292015210 90251015210 90254015210 90258015210 90259015210 90267015210 90218015210 90222015210 90223015210 90225015210 90227015210 Factors Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Control | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:1 week of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:2 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:4 weeks of training | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Female | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder Group:Training | Timepoint:8 weeks of training or control time | Sex:Male | Tissue:Rat Hippocampus Powder 2-Hydroxyglutarate 0.0788 0.0639 0.0699 0.0784 0.1284 0.0793 0.0906 0.1144 0.0712 0.0914 0.0703 0.0568 0.0732 0.0672 0.0778 0.0787 0.0930 0.0673 0.0594 0.0707 0.0698 0.0885 0.0774 0.0936 0.0852 0.1286 0.0829 0.0685 0.0976 0.0753 0.0772 0.0719 0.0035 0.1067 0.0897 0.1060 0.0740 0.1198 0.0642 0.0820 0.0819 0.0785 0.0712 0.0598 0.0625 Aspartate 6.9610 7.1716 7.7854 7.8040 8.4627 8.4957 7.9506 8.3917 7.9671 7.4594 7.5005 6.6386 6.7565 6.8051 7.1209 7.6289 8.5721 6.8377 6.5848 7.4588 7.3829 7.6210 7.5202 8.3288 7.9415 9.2402 7.9867 7.4359 8.6017 7.1245 8.7455 7.5779 1.9077 8.9378 7.9782 7.0213 6.5716 8.5589 7.0726 8.1621 7.1199 7.6822 7.8880 7.1188 6.9840 cis-aconitate 0.0230 0.0185 0.0248 0.0255 0.0259 0.0299 0.0280 0.0287 0.0281 0.0257 0.0286 0.0291 0.0290 0.0253 0.0278 0.0328 0.0274 0.0266 0.0276 0.0323 0.0324 0.0288 0.0325 0.0338 0.0189 0.0407 0.0302 0.0344 0.0354 0.0267 0.0312 0.0262 0.0129 0.0304 0.0285 0.0254 0.0197 0.0320 0.0254 0.0267 0.0228 0.0264 0.0245 0.0252 0.0223 Citrate 1.1498 0.7484 1.1514 1.2582 1.1274 1.1864 1.1880 1.0430 1.2982 1.0282 1.2448 1.2354 0.9015 0.7848 0.8804 1.3924 1.0581 0.9156 1.0834 1.0084 1.4408 0.9504 1.4129 1.3864 1.0210 1.7041 0.9800 1.3361 1.2472 0.8554 1.4317 1.2719 0.2683 1.4865 1.0671 0.9940 0.7850 1.3216 1.1946 1.0941 0.8194 1.1052 1.2136 1.0874 0.9864 Fumarate 1.3604 0.9219 1.3391 1.3703 1.5025 1.5620 1.5333 1.6064 1.3948 1.1846 1.3601 1.0528 1.0298 1.1256 1.2805 1.5122 1.6559 1.2590 1.0962 1.3008 1.2267 1.3093 1.5322 1.7485 2.1372 1.7724 1.4394 1.3014 1.7877 1.1882 1.5894 1.3656 0.2511 1.5135 1.5866 1.2370 0.9924 1.8662 1.2878 1.4284 1.2763 1.5047 1.4998 1.2244 1.2461 Glutamate 37.1910 36.8961 38.0025 38.3775 43.1721 42.1438 40.3391 42.7858 39.3943 43.0985 38.6103 36.1757 40.0918 37.7269 40.9407 41.3424 47.8519 39.7250 33.8957 40.7207 38.0088 40.4140 40.7897 44.9732 40.8827 46.5466 43.4652 38.8466 45.1599 39.6041 43.1970 38.4825 12.6689 43.6287 42.2044 38.2350 36.3218 43.5142 37.1471 40.1319 37.2535 39.2265 39.7208 37.6053 37.2870 Isocitrate 0.0627 0.0411 0.0652 0.0677 0.0608 0.0696 0.0622 0.0567 0.0733 0.0570 0.0591 0.0608 0.0485 0.0432 0.0463 0.0666 0.0594 0.0477 0.0558 0.0537 0.0702 0.0497 0.0671 0.0658 0.0520 0.0784 0.0539 0.0709 0.0674 0.0469 0.0752 0.0705 0.0215 0.0774 0.0557 0.0552 0.0428 0.0724 0.0663 0.0601 0.0445 0.0590 0.0643 0.0623 0.0539 Ketoglutarate 0.3361 0.3271 0.2805 0.2551 0.2397 0.4080 0.3189 0.3270 0.3342 0.2225 0.3693 0.3296 0.3239 0.4234 0.4514 0.4179 0.5397 0.4671 0.3382 0.2969 0.3154 0.4086 0.3564 0.3150 2.2828 0.2699 0.3679 0.3617 0.3692 0.4187 0.2038 0.2279 0.2833 0.1745 0.3672 0.2966 0.2933 0.2618 0.3041 0.3069 0.3471 0.3721 0.4035 0.3611 0.3767 Lactate 83.1507 67.2642 80.7444 90.4626 80.4112 106.6875 92.8735 97.2406 87.7588 89.9143 89.6841 73.8691 63.4721 73.2265 79.7215 86.2232 87.7070 75.0262 75.5400 73.9125 75.6806 75.1092 87.0653 89.3699 84.6168 98.9379 82.0369 81.2036 87.6493 71.3695 94.6745 86.1442 17.6808 98.8109 82.0597 85.9534 77.4441 98.5858 80.2826 85.2360 81.1653 92.9967 91.2845 86.5558 86.1259 Malate 2.5250 1.8589 2.4647 2.5060 2.4957 3.1126 2.8875 2.8137 2.5489 2.8419 2.8669 2.1783 2.2149 2.1625 2.4086 2.9537 2.9284 2.3953 2.2570 2.5589 2.3717 2.4249 2.9476 3.2231 2.5847 3.3856 2.6501 2.5008 3.2710 2.2302 2.8751 2.5342 0.5683 3.0791 2.9240 2.4231 2.1120 3.1996 2.3410 2.5248 2.2590 2.7599 2.7532 2.4678 2.5167 Oxaloacetate Succinate 0.2402 1.1786 0.3485 0.2318 1.4696 0.3050 0.2446 1.7177 0.1934 1.2211 0.1892 0.1272 1.5801 1.4835 1.8212 0.2976 1.7520 1.4883 0.2148 1.2573 0.1733 1.6721 0.2380 0.6312 1.6753 0.1906 1.8087 0.1749 1.0689 1.4959 0.3721 0.3163 0.0405 0.2970 1.1749 1.0931 1.0685 0.8978 0.2360 1.0201 1.0015 0.4288 0.2874 0.1379 0.3378 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 2-Hydroxyglutarate 9.98 433 Aspartate 9.37 418 cis-aconitate 14.08 459 Citrate 14.09 591 Fumarate 6.13 287 Glutamate 10.58 432 Isocitrate 14.09 591 Ketoglutarate 8.37 360 Lactate 4.18 261 Malate 8.93 419 Oxaloacetate 8.37 346 Succinate 5.19 289 METABOLITES_END #END