#METABOLOMICS WORKBENCH efahy_20230425_000016 DATATRACK_ID:3908 STUDY_ID:ST002597 ANALYSIS_ID:AN004269 PROJECT_ID:PR001020 VERSION 1 CREATED_ON April 25, 2023, 7:56 am #PROJECT PR:PROJECT_TITLE Molecular Transducer of Physical Activity Consortium PR:PROJECT_SUMMARY MoTrPAC is a national research consortium designed to discover and perform PR:PROJECT_SUMMARY preliminary characterization of the range of molecular transducers (the PR:PROJECT_SUMMARY "molecular map") that underlie the effects of physical activity in humans. The PR:PROJECT_SUMMARY program's goal is to study the molecular changes that occur during and after PR:PROJECT_SUMMARY exercise and ultimately to advance the understanding of how physical activity PR:PROJECT_SUMMARY improves and preserves health. Preclinical and clinical studies will examine the PR:PROJECT_SUMMARY systemic effects of endurance and resistance exercise across a range of ages and PR:PROJECT_SUMMARY fitness levels by molecular probing of multiple tissues before and after acute PR:PROJECT_SUMMARY and chronic exercise. This program is the largest targeted NIH investment of PR:PROJECT_SUMMARY funds into the mechanisms of how physical activity improves health and prevents PR:PROJECT_SUMMARY disease. The MoTrPAC program is supported by the NIH Common Fund and is managed PR:PROJECT_SUMMARY by a trans-agency working group representing multiple NIH institutes and PR:PROJECT_SUMMARY centers, led by the NIH Office of Strategic Coordination, National Institute of PR:PROJECT_SUMMARY Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes PR:PROJECT_SUMMARY and Digestive and Kidney Diseases, National Institute on Aging, and National PR:PROJECT_SUMMARY Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: PR:PROJECT_SUMMARY Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion PR:PROJECT_SUMMARY Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac PR:PROJECT_SUMMARY Bioinformatics / Data Coordination Center. PR:INSTITUTE MoTrPAC PR:LAST_NAME Ashley PR:FIRST_NAME Euan A. PR:ADDRESS 300 Pasteur Dr Rm A265. MC 5319. Stanford CA 94305. USA PR:EMAIL euan@stanford.edu PR:PHONE (650) 498-4900 #STUDY ST:STUDY_TITLE MoTrPAC: Endurance exercise training study in young adult rats, Rat ST:STUDY_TITLE Gastrocnemius Powder - Targeted Tricarboxylic Acid Cycle ST:STUDY_SUMMARY The goal of the endurance exercise training study in young adult rats (internal ST:STUDY_SUMMARY code: PASS1B-06) was to perform exercise training studies in adult (6 month) ST:STUDY_SUMMARY F344 rats, and from these rats collect as many tissues as feasible in order to ST:STUDY_SUMMARY provide high quality samples for detailed analysis by chemical analysis sites. ST:STUDY_SUMMARY Tissues were collected from 10-12 rats sedentary control rats concurrent with ST:STUDY_SUMMARY the collection of the 8-week training groups. The 8-week training group and ST:STUDY_SUMMARY controls were from the same cohort and same age at euthanasia (either 8). For ST:STUDY_SUMMARY the older age group, an additional set of controls (n=5-6) were collected with ST:STUDY_SUMMARY the 1-2 week training group. Rats were either sedentary or underwent an exercise ST:STUDY_SUMMARY training program. Rats were exercised on the rodent treadmill 5 days per week ST:STUDY_SUMMARY using a progressive training protocol designed to exercise the rats at ST:STUDY_SUMMARY approximately 70% of VO2max as outlined in the Table on the next page. Training ST:STUDY_SUMMARY was performed no earlier than 10:00 am and no later than 5:00 pm over 5 ST:STUDY_SUMMARY consecutive days per week. Training was initiated with the treadmill set at 70% ST:STUDY_SUMMARY of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of ST:STUDY_SUMMARY exercise was increased by one minute each day until day 31 of training (start of ST:STUDY_SUMMARY week 7), when a duration of 50 min was reached. Speed and grade of each training ST:STUDY_SUMMARY session increased in larger increments due to treadmill parameters. The highest ST:STUDY_SUMMARY intensity and duration of training began on day 31. This intensity was ST:STUDY_SUMMARY maintained for the final 10 days of the protocol to ensure steady state had been ST:STUDY_SUMMARY achieved. If any rats were unable to perform at least 4 days of training per ST:STUDY_SUMMARY week they were removed from the study and euthanized. It is important to note ST:STUDY_SUMMARY that the starting treadmill speed varied depending on the sex and age of the ST:STUDY_SUMMARY rat. The initial and maximum speeds were based on VO2max measurements obtained ST:STUDY_SUMMARY during the pre-training testing of the compliant rats. Rats assigned to the ST:STUDY_SUMMARY control group followed a schedule similar to the training group. They were ST:STUDY_SUMMARY placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The ST:STUDY_SUMMARY treadmill was set at 0 m/min at an incline that corresponded to the incline ST:STUDY_SUMMARY being used by the training group. ST:INSTITUTE Mayo Clinic ST:DEPARTMENT Internal Medicine ST:LABORATORY Mayo Clinic Metabolomics Resource Core (MCMRC) ST:LAST_NAME Nair ST:FIRST_NAME K. Sreekumaran ST:ADDRESS 200 1st St SW, Rochester, MN 55902 ST:EMAIL nair@mayo.edu ST:PHONE (507) 284-2511 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS BC49E4EF-E2FE-4269-BAF0-461F768BD95D 90217015516 Group:Control | Timepoint:8 weeks of training or control time | Sex:Male raw_file=31dec20_30.D.zip SUBJECT_SAMPLE_FACTORS 0BD282C7-7832-4581-9089-08A32D557034 90218015516 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male raw_file=31dec20_31.D.zip SUBJECT_SAMPLE_FACTORS 955498F4-70A0-4D50-A2BA-619A8FED8FEF 90222015516 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male raw_file=31dec20_32.D.zip SUBJECT_SAMPLE_FACTORS CFE06932-9314-4BCC-B2D9-73883D5FCDFB 90223015516 Group:Training | Timepoint:8 weeks of training or control time | Sex:Male 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| Timepoint:2 weeks of training | Sex:Female raw_file=06jan21_44.D.zip SUBJECT_SAMPLE_FACTORS DC8B35E8-4AA8-4829-8569-F223D069620C 90578015516 Group:Training | Timepoint:2 weeks of training | Sex:Female raw_file=06jan21_45.D.zip SUBJECT_SAMPLE_FACTORS 8904857C-2073-4BBA-AC7C-1EC239E5BFF7 90581015516 Group:Training | Timepoint:2 weeks of training | Sex:Female raw_file=06jan21_46.D.zip SUBJECT_SAMPLE_FACTORS 1F9BD644-303F-43E7-94BE-FF6654306F5E 90585015516 Group:Training | Timepoint:2 weeks of training | Sex:Female raw_file=06jan21_47.D.zip SUBJECT_SAMPLE_FACTORS 0409BED3-F9FE-4C06-9920-74F5E5CA04A3 90587015516 Group:Training | Timepoint:2 weeks of training | Sex:Female raw_file=06jan21_48.D.zip #COLLECTION CO:SAMPLE_TYPE Gastrocnemius #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with SP:SAMPLEPREP_SUMMARY ethoxamine, MTBSTFA #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Analytes were chromatographically separated with 17 minutes GC gradient CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent Intuvo 9000 GC CH:COLUMN_NAME Agilent DB5-MS (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_TYPE Single quadrupole MS:INSTRUMENT_NAME Agilent 5977B HES MSD MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS SIM; peak integration, concentrations calculated #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nano moles per vial MS_METABOLITE_DATA_START Samples 90217015516 90218015516 90222015516 90223015516 90225015516 90227015516 90229015516 90232015516 90237015516 90239015516 90245015516 90248015516 90251015516 90252015516 90254015516 90258015516 90259015516 90265015516 90266015516 90267015516 90280015516 90281015516 90283015516 90289015516 90292015516 90406015516 90410015516 90412015516 90416015516 90420015516 90421015516 90422015516 90423015516 90426015516 90430015516 90439015516 90441015516 90444015516 90449015516 90450015516 90559015516 90560015516 90564015516 90567015516 90571015516 90576015516 90578015516 90581015516 90585015516 90587015516 Lactate 175.5684 141.486 100.8468 121.0582 159.5499 106.4184 177.0209 128.978 144.3399 142.2756 145.6647 173.9551 140.338 125.6178 111.0186 178.0178 127.78 142.2978 120.1617 129.8017 156.8862 129.4625 173.7265 149.8852 139.8947 130.9426 100.2845 157.272 148.4046 144.1254 143.2513 172.7859 152.9705 143.3105 149.5608 117.1209 158.0097 151.5318 180.5025 182.7405 150.6336 165.0185 141.3054 160.0142 156.3811 141.1265 166.9966 159.3763 156.8491 172.1284 Succinate 0.198 0.1934 0.14 0.1847 0.2345 0.1562 0.2339 0.1282 0.3833 0.13 0.1772 0.181 0.2185 0.4613 0.201 0.2641 0.1622 0.2218 0.1605 0.3503 0.2208 0.4402 0.2606 0.177 0.1897 0.1958 0.2014 0.1622 0.16 0.2031 0.1818 0.1929 0.1448 0.167 0.181 0.1477 0.1586 0.2063 0.2077 0.2576 0.1747 0.2197 0.2177 0.1991 0.1356 0.1566 0.2007 0.1576 0.1919 0.204 Fumarate 1.106 1.12 0.717 0.9117 1.1726 0.8449 1.2548 0.8154 0.874 0.7323 0.9369 0.8208 1.2266 0.7541 0.75 1.2596 0.6751 0.6333 0.7295 0.8421 1.024 0.8515 0.9903 0.9214 0.8123 0.5424 0.3617 0.5624 0.6162 0.7216 0.6806 0.6731 0.5031 0.6404 0.6638 0.6608 0.4968 0.8803 0.8723 0.8926 0.5089 0.6688 0.6027 0.5894 0.4386 0.373 0.7763 0.4128 0.6582 0.5273 Oxaloacetate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ketoglutarate 0.1982 0.0937 0.0818 0.074 0.1399 0.0681 0.1903 0.1015 0.1075 0.0754 0.086 0.0943 0.1822 0.0743 0.1202 0.1274 0.1171 0.0931 0.1895 0.0598 0.1372 0.0804 0.1307 0.1362 0.1149 0.028 0.0338 0.0699 0.042 0.045 0.0433 0.071 0.0536 0.0473 0.0498 0.0222 0.043 0.0346 0.0459 0.0649 0.0335 0.0768 0.0447 0.03 0.0438 0.0225 0.0529 0.0686 0.0655 0.0816 Malate 1.6553 1.581 1.2441 1.3549 1.717 1.2249 2.1338 1.232 1.1929 1.276 1.9316 1.5142 1.788 1.4018 1.2327 2.1496 1.244 1.0942 1.4525 1.4333 1.6257 1.2739 1.6208 1.4247 1.1906 1.4401 0.8079 1.4073 1.3703 1.7687 1.5152 1.614 1.1849 1.4549 1.4708 1.3325 1.1787 1.9289 2.4076 1.9286 1.4715 1.5609 1.5831 1.6309 1.2852 2.0994 1.7929 1.6608 1.6866 1.7476 Aspartate 1.1045 1.9732 1.4563 1.5318 1.9842 1.2048 1.6713 1.1386 2.1118 1.491 1.4544 1.406 2.1281 1.6526 2.0137 1.7162 1.1736 0.9992 0.9142 1.5136 2.229 1.885 1.5505 1.8654 2.2393 1.8747 1.1033 1.3139 1.7951 1.5254 1.5256 1.23 1.1424 1.6092 1.3489 1.7345 1.0087 2.1056 2.0764 2.1671 1.8715 1.8805 2.0479 0.7911 1.179 1.4189 1.8661 1.0989 1.2086 0.9111 2-Hydroxyglutarate 0.0238 0.0178 0.0423 0.0191 0.0161 0.0125 0.0216 0.013 0.0167 0.0123 0.0184 0.0197 0.015 0.02 0.0186 0.0184 0.0131 0.0137 0.0211 0.012 0.0163 0.0149 0.015 0.011 0.0127 0.0162 0.0095 0.0151 0.0238 0.0222 0.0144 0.02 0.0119 0.0141 0.0157 0.0146 0.0112 0.017 0.02 0.0224 0.0217 0.0227 0.0175 0.0142 0.0119 0.0151 0.0184 0.0134 0.0174 0.0164 Glutamate 7.4855 6.7173 5.2398 5.2922 6.9833 4.3 6.5033 5.846 6.9188 4.534 5.4932 6.4487 6.2517 6.6139 6.2337 6.4741 5.6068 5.2098 6.3347 5.1222 5.6876 5.948 5.9304 6.3272 5.5716 5.4138 4.3296 5.0402 5.6136 5.775 4.5248 5.6807 3.4468 5.259 4.6514 3.9369 4.3315 5.6948 5.665 6.1618 3.7565 6.1222 5.7681 4.9405 3.6057 5.55 6.0231 5.0948 6.0451 5.4759 cis-aconitate 0.0226 0.0205 0.0184 0.0227 0.0305 0.0244 0.0248 0.0185 0.0232 0.0153 0.0219 0.017 0.0262 0.0225 0.0221 0.0267 0.0257 0.0185 0.0159 0.0222 0.0273 0.0211 0.029 0.019 0.0242 0.0218 0.0141 0.0233 0.0206 0.0159 0.0182 0.0272 0.0188 0.0233 0.0209 0.0278 0.0272 0.0237 0.0235 0.03 0.02 0.0204 0.0271 0.0357 0.0168 0.0195 0.0252 0.0187 0.0224 0.022 Citrate 0.4007 0.4576 0.5298 0.535 0.5328 0.3937 0.5461 0.3341 0.3705 0.3283 0.474 0.4871 0.4812 0.3821 0.4342 0.5824 0.4215 0.2997 0.4193 0.4131 0.4804 0.3565 0.554 0.4584 0.3495 0.2767 0.2176 0.2963 0.3325 0.2987 0.2778 0.3262 0.2702 0.3345 0.3576 0.2894 0.2824 0.3577 0.4633 0.3506 0.2941 0.3813 0.3055 0.4406 0.2767 0.2745 0.4018 0.3477 0.3187 0.3581 Isocitrate 0.035 0.0394 0.0383 0.0466 0.046 0.0471 0.0452 0.0326 0.0365 0.0309 0.0427 0.0329 0.0461 0.0325 0.0393 0.05 0.0391 0.0294 0.0337 0.0375 0.0443 0.0325 0.0471 0.0419 0.0338 0.0273 0.0209 0.0264 0.0308 0.0291 0.0248 0.0309 0.0234 0.0303 0.0313 0.0279 0.0298 0.0309 0.0381 0.0321 0.0238 0.0305 0.0282 0.0379 0.0241 0.0224 0.0318 0.0276 0.0271 0.0319 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name formula mz neutral_mass refmet_name rt Lactate C3H6O3 261 90.0317 Lactic acid 4.18 Succinate C4H6O4 289 118.0266 Succinic acid 5.19 Fumarate C4H4O4 287 116.011 Fumaric acid 6.13 Oxaloacetate C4H4O5 346 132.0059 Oxaloacetic acid 8.37 Ketoglutarate C5H6O5 360 146.0215 Oxoglutaric acid 8.37 Malate C4H6O5 419 134.0215 Malic acid 8.93 Aspartate C4H7NO4 418 133.0375 Aspartic acid 9.37 2-Hydroxyglutarate C5H8O5 433 148.114 2-Hydroxyglutaric acid 9.98 Glutamate C5H9NO4 432 147.0532 Glutamic acid 10.58 cis-aconitate C6H6O6 459 174.0164 cis-Aconitic acid 14.08 Citrate C6H8O7 591 192.027 Citric acid 14.09 Isocitrate C6H8O7 591 192.027 Isocitric acid 14.09 METABOLITES_END #END