#METABOLOMICS WORKBENCH efahy_20130215_9775041_mwtab.txt DATATRACK_ID:36 STUDY_ID:ST000004 ANALYSIS_ID:AN000007 PROJECT_ID:PR000004
VERSION             	1
CREATED_ON          	2016-09-17
#PROJECT
PR:PROJECT_TITLE                 	LIPID MAPS Lipidomics studies
PR:PROJECT_TYPE                  	MS quantitative analysis
PR:PROJECT_SUMMARY               	Multi-center quantitative lipidomics studies on samples from human and murine
PR:PROJECT_SUMMARY               	(LIPIDMAPS)
PR:INSTITUTE                     	LIPID MAPS
PR:DEPARTMENT                    	Multiple
PR:LABORATORY                    	Multiple
PR:LAST_NAME                     	Fahy
PR:FIRST_NAME                    	Eoin
PR:ADDRESS                       	UCSD
PR:EMAIL                         	efahy@ucsd.edu
PR:PHONE                         	-
PR:FUNDING_SOURCE                	NIGMS
#STUDY
ST:STUDY_TITLE                   	Lipidomics studies on NIDDK / NIST human plasma samples
ST:STUDY_TYPE                    	MS analysis on human plasma
ST:STUDY_SUMMARY                 	The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) in
ST:STUDY_SUMMARY                 	with the National Institute of Standards (NIST) recently produced a human
ST:STUDY_SUMMARY                 	standard reference material (SRM 1950) for metabolite analysis. The SRM was
ST:STUDY_SUMMARY                 	by obtaining plasma samples from 100 individuals between 40 and 50 years of
ST:STUDY_SUMMARY                 	whose ethnicity was representative of the US population and that included an
ST:STUDY_SUMMARY                 	number of men and women. The intent of the NIDDK/NIST project was to provide a
ST:STUDY_SUMMARY                 	material that would be publically available to researchers and that could be
ST:STUDY_SUMMARY                 	by the clinical chemistry community to identify plasma metabolites for
ST:STUDY_SUMMARY                 	purposes. Signature metabolites could then be further probed for their
ST:STUDY_SUMMARY                 	as disease biomarkers. The LIPID MAPS Consortium has undertaken the task to
ST:STUDY_SUMMARY                 	this SRM by systematically identifying and quantifying the lipid molecular
ST:STUDY_SUMMARY                 	in the six main categories of mammalian lipids. The quantitative levels of over
ST:STUDY_SUMMARY                 	different lipids present in this reference human plasma sample are presented
ST:INSTITUTE                     	LIPID MAPS
ST:DEPARTMENT                    	Multiple
ST:LABORATORY                    	Multiple
ST:LAST_NAME                     	Fahy
ST:FIRST_NAME                    	Eoin
ST:ADDRESS                       	UCSD
ST:EMAIL                         	efahy@ucsd.edu
ST:PHONE                         	-
ST:NUM_GROUPS                    	1
ST:TOTAL_SUBJECTS                	100
ST:NUM_MALES                     	100
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	40-50
SU:SPECIES_GROUP                 	Human
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	NIDDK1	Pooled sample:yes	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	-
CH:COLUMN_NAME                   	-
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	-
MS:INSTRUMENT_TYPE               	-
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	-
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nmol/ml
MS_METABOLITE_DATA_START
Samples	NIDDK1
Factors	Pooled sample:yes
24S-hydroxy-cholesterol	0.0500
25-hydroxy-cholesterol	0.0167
27-hydroxy-cholesterol	0.5333
4beta-hydroxy-cholesterol	0.0533
7alpha-hydroxy-cholesterol	0.3333
7-Dehydrocholesterol	3.0033
7-oxo-cholesterol	0.1233
Campesterol	6.4600
Cholestenone	0.0767
Cholesterol	3150.3667
Desmosterol	1.8067
Lanosterol	0.4833
Lathosterol	5.3767
Sitosterol	5.5333
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
24S-hydroxy-cholesterol				121948		C13550		
25-hydroxy-cholesterol				65094		C15519		
27-hydroxy-cholesterol				99470		C15610		
4beta-hydroxy-cholesterol				3247060				
7alpha-hydroxy-cholesterol				107722		C03594		
7-Dehydrocholesterol				439423		C01164		
7-oxo-cholesterol				91474				
Campesterol				5283637		C01789		
Cholestenone				9908107		C00599		
Cholesterol				5997		C00187		
Desmosterol				313036		C01802		
Lanosterol				246983		C01724		
Lathosterol				65728		C01189		
Sitosterol				222284		C01753		
METABOLITES_END
#END