#METABOLOMICS WORKBENCH wpathmasiri_20140314_9890851_mwtab.txt DATATRACK_ID:69 TEXT OUTPUT STUDY_ID:ST000037 ANALYSIS_ID:AN000057 PROJECT_ID:PR000025
VERSION             	1
CREATED_ON          	10-02-2015
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics Involved in Early Life Antibiotic Exposures
PR:PROJECT_TYPE                  	Obesity modeling of antibiotic exposure
PR:PROJECT_SUMMARY               	The project Metabolomics Involved in Early Life Antibiotic Exposures profiled a
PR:PROJECT_SUMMARY               	total of 90 samples from five sub-studies (DuraSTAT, TranSTAT, NOD, EstroSTAT
PR:PROJECT_SUMMARY               	and VG STAT) which included a total of four sample matrices (urine, serum, liver
PR:PROJECT_SUMMARY               	tissue and cecal contents). Within each sub-study, there were three sample
PR:PROJECT_SUMMARY               	matrices except for VG STAT, for which there was only two. For each matrix type
PR:PROJECT_SUMMARY               	within each sub-study 6 samples were analyzed for a total of 18 samples per
PR:PROJECT_SUMMARY               	sub-study (9 of each in VG STAT), the samples were equally divided into
PR:PROJECT_SUMMARY               	STAT/PAT-treated (sub-therapeutic antibiotic treatment (STAT) and therapeutic
PR:PROJECT_SUMMARY               	dose-pulsed antibiotic treatment (PAT)) collected at various time-points versus
PR:PROJECT_SUMMARY               	untreated Controls for each matrix.
PR:INSTITUTE                     	New York University
PR:DEPARTMENT                    	School of Medicine
PR:LABORATORY                    	Blaser Laboratory
PR:LAST_NAME                     	Blaser
PR:FIRST_NAME                    	Martin J.
PR:ADDRESS                       	550 First Avenue, BCD 690, New York, NY 10016
PR:EMAIL                         	Martin.Blaser@nyumc.org
PR:PUBLICATIONS                  	Cho I, Blaser MJ. The human microbiome: at the interface of health and disease.
PR:PUBLICATIONS                  	Nature Reviews. Genetics 2012; 13; 260-270
PR:PHONE                         	-
#STUDY
ST:STUDY_TITLE                   	Metabolomics Involved in Early Life Antibiotic Exposures(EstroSTAT-Urine)
ST:STUDY_TYPE                    	Metabolomics
ST:STUDY_SUMMARY                 	In the EstroSTAT sub-study, a total of 18 samples from 23 week old, female
ST:STUDY_SUMMARY                 	C57BL/6 mice; comprised of 6 urine samples, 6 serum samples and 6 liver tissue
ST:STUDY_SUMMARY                 	samples were analyzed. Three mice/matrix were given STAT penicillin and 3
ST:STUDY_SUMMARY                 	mice/matrix were non-treated Controls. The mice were housed with conventional
ST:STUDY_SUMMARY                 	bedding and fed a Low phyto-estrogen diet.
ST:INSTITUTE                     	RTI International
ST:DEPARTMENT                    	Systems and Translational Sciences (STS)
ST:LABORATORY                    	NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)
ST:LAST_NAME                     	Sumner
ST:FIRST_NAME                    	Susan
ST:EMAIL                         	ssumner@rti.org
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	6
ST:STUDY_COMMENTS                	EstroSTAT_Urine
ST:PHONE                         	-
ST:ADDRESS                       	-
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6
SU:GENDER                        	Female
SU:ANIMAL_HOUSING                	Conventional
SU:ANIMAL_FEED                   	Low phyto-estrogen diet
SU:SPECIES_GROUP                 	Mammal
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	UEC906	Treatment Group:Control	
SUBJECT_SAMPLE_FACTORS           	-	UEC920	Treatment Group:Control	
SUBJECT_SAMPLE_FACTORS           	-	UEC922	Treatment Group:Control	
SUBJECT_SAMPLE_FACTORS           	-	UPL101	Treatment Group:Pooled QC	
SUBJECT_SAMPLE_FACTORS           	-	UPL102	Treatment Group:Pooled QC	
SUBJECT_SAMPLE_FACTORS           	-	UPL103	Treatment Group:Pooled QC	
SUBJECT_SAMPLE_FACTORS           	-	UES365	Treatment Group:STAT	
SUBJECT_SAMPLE_FACTORS           	-	UES910	Treatment Group:STAT	
SUBJECT_SAMPLE_FACTORS           	-	UES911	Treatment Group:STAT	
#COLLECTION
CO:SAMPLE_TYPE                   	urine
CO:COLLECTION_TIME               	23 weeks
CO:COLLECTION_SUMMARY            	-
#TREATMENT
TR:TREATMENT_SUMMARY             	Three mice were given STAT penicillin and 3 mice were non-treated controls. STAT
TR:TREATMENT_SUMMARY             	= sub-therapeutic antibiotic treatment.
TR:TREATMENT_COMPOUND            	STAT Penicillin
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Frozen urine study samples were thawed on ice and vortexed for 30 seconds.
SP:SAMPLEPREP_SUMMARY            	Aliquots of 400 µL were transferred into BSI-labeled eppendorf tubes. Aliquots
SP:SAMPLEPREP_SUMMARY            	of 100 µL per study sample were also transferred into a 10 mL tube to generate
SP:SAMPLEPREP_SUMMARY            	pooled samples for QC during analysis, and 400 µL was transferred into
SP:SAMPLEPREP_SUMMARY            	BSI-labeled eppendorf tubes. D2O (230 µL) was added to each tube.  Chenomx
SP:SAMPLEPREP_SUMMARY            	Internal Standard solution (Chenomx ISTD, Edmonton, Alberta, Canada) contains
SP:SAMPLEPREP_SUMMARY            	5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator),
SP:SAMPLEPREP_SUMMARY            	100 mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) was
SP:SAMPLEPREP_SUMMARY            	added (70 µl) to the tubes. Tubes were vortexed for 30 seconds and centrifuged
SP:SAMPLEPREP_SUMMARY            	at 12000 rcf for 5min. A 600 µL aliquot of the supernatant was transferred into
SP:SAMPLEPREP_SUMMARY            	5mm NMR tubes (Bruker-Biospin, Germany), which were kept on ice until data
SP:SAMPLEPREP_SUMMARY            	acquisition.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	EstroSTAT_Urine_Metabolomics_Procedure.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	-
CH:COLUMN_NAME                   	-
#ANALYSIS
AN:LABORATORY_NAME               	RTI/ DHHMRI
AN:ANALYSIS_TYPE                 	NMR
AN:ACQUISITION_DATE              	41527
AN:SOFTWARE_VERSION              	Top Spin 3.2
AN:OPERATOR_NAME                 	Wimal Pathmasiri/ Kevin Knagge/ Jason Winnikie
AN:DETECTOR_TYPE                 	NMR
AN:DATA_FORMAT                   	NMR
#NMR
NM:INSTRUMENT_TYPE               	Bruker Avance III
NM:NMR_EXPERIMENT_TYPE           	1D 1H
NM:FIELD_FREQUENCY_LOCK          	Deuterium
NM:STANDARD_CONCENTRATION        	0.5mM
NM:SPECTROMETER_FREQUENCY        	700 MHz
NM:NMR_PROBE                     	cyrogenically cooled ATMA inverse probe
NM:NMR_SOLVENT                   	D2O
NM:NMR_TUBE_SIZE                 	5mm x 4inch
NM:SHIMMING_METHOD               	topshim
NM:PULSE_SEQUENCE                	noesypr1d
NM:WATER_SUPPRESSION             	presat
NM:PULSE_WIDTH                   	14.84 us
NM:POWER_LEVEL                   	25.704w
NM:RECEIVER_GAIN                 	9
NM:OFFSET_FREQUENCY              	3296 Hz
NM:CHEMICAL_SHIFT_REF_CPD        	DSS
NM:TEMPERATURE                   	298.1 K
NM:NUMBER_OF_SCANS               	64
NM:DUMMY_SCANS                   	4
NM:ACQUISITION_TIME              	2.3243434
NM:RELAXATION_DELAY              	2 s
NM:SPECTRAL_WIDTH                	20.1358
NM:NUM_DATA_POINTS_ACQUIRED      	65536
NM:REAL_DATA_POINTS              	65536
NM:LINE_BROADENING               	0.5
NM:ZERO_FILLING                  	yes
NM:APODIZATION                   	lorentzian
NM:BASELINE_CORRECTION_METHOD    	polynomial
NM:CHEMICAL_SHIFT_REF_STD        	DSS
NM:INSTRUMENT_NAME               	Bruker Avance III
#NMR_BINNED_DATA
NMR_BINNED_DATA:UNITS           	Peak area
NMR_BINNED_DATA_START
Bin range(ppm)	UEC906	UEC920	UEC922	UPL101	UPL102	UPL103	UES365	UES910	UES911
0.50...0.52	0	0	0	0	0	0.11919	0	0	0.15588
0.52...0.54	0.0014553	0	0	0.0069551	0.0032387	0.1385	0.0014973	0.0046831	0.17698
0.54...0.56	0	0	0	0	0.00084044	0.15868	0	0	0.18816
0.56...0.59	0.023451	0.00083818	0.0024418	0	0	0.21503	0	0	0.22199
0.59...0.64	1.7716	2.3134	1.3746	1.3744	1.4471	2.1682	1.704	1.1775	1.6987
0.64...0.67	0.26985	0.00038509	0.16267	0	0	0.34144	0.0010142	0.16342	0.29932
0.67...0.69	0.26581	0	0.20763	0	0	0.40104	0	0.21559	0.3423
0.69...0.73	0.79715	0.0081611	0.60946	0.0076582	0.028258	1.0425	0.012546	0.67008	0.81203
0.73...0.75	0.42763	0	0.36807	0.031243	0	0.61349	0	0.39641	0.47257
0.75...0.77	0.56239	0	0.45326	0.15343	0.016193	0.80703	0.0052327	0.56054	0.60687
0.77...0.79	0.56739	0.020577	0.45754	0.1865	0.033895	0.76903	0	0.54863	0.6129
0.79...0.81	0.62437	0.19076	0.52913	0.41546	0.26018	0.94311	0.021382	0.62289	0.66663
0.81...0.83	0.85184	0.50622	0.85701	0.78209	0.63715	1.2991	0.26066	0.93806	0.96042
0.83...0.89	8.4352	7.8606	9.5528	9.6198	9.381	10.639	6.5667	9.3157	8.0948
0.89...0.95	15.241	13.597	16.353	16.18	16.186	17.037	12.083	16.669	13.687
0.95...1.00	3.3666	2.4391	4.021	2.811	2.6514	4.068	2.0494	3.6929	3.1759
1.00...1.02	0.86894	0.36325	0.90818	0.44474	0.3408	0.9748	0.39483	0.85039	0.88386
1.02...1.07	2.8029	1.5057	2.88	1.5552	1.3063	2.7926	1.6473	2.5254	2.7201
1.07...1.13	6.204	4.1049	6.1787	4.5145	4.2775	5.7604	4.9113	6.0866	5.7242
1.13...1.16	1.8605	1.4281	2.055	1.2813	1.1233	1.9586	1.2978	1.7737	1.8966
1.16...1.22	5.7713	4.9119	6.1878	4.9649	4.7369	6.1514	4.8572	5.6544	5.6412
1.22...1.25	3.9066	3.1272	4.0994	3.2362	3.1172	3.8286	3.4703	3.631	3.7238
1.25...1.28	2.2547	2.021	2.2624	1.8783	1.7692	2.327	1.8936	2.1615	2.32
1.28...1.33	8.1429	7.5778	8.892	7.6118	7.3908	8.636	7.042	7.5893	7.2727
1.33...1.37	3.9635	3.7587	4.3485	3.5656	3.477	4.1724	3.9763	4.1723	4.2822
1.37...1.40	2.2509	1.9262	2.4059	2.2666	2.1615	2.785	1.8716	2.3493	2.4997
1.40...1.42	1.5399	1.2977	1.7253	1.3946	1.3227	1.8013	1.2358	1.6008	1.5938
1.42...1.46	4.087	3.5309	4.9065	3.7076	3.575	4.5088	3.5523	4.3689	4.4912
1.46...1.49	3.3196	2.6702	3.5133	2.8681	2.7644	3.4551	2.6458	3.2955	3.3353
1.49...1.51	1.615	1.3495	1.7597	1.4149	1.333	1.7815	1.392	1.6783	1.7707
1.51...1.54	2.1723	1.9583	2.3224	1.9634	1.8662	2.3187	1.975	2.1932	2.2079
1.54...1.56	2.4246	2.2012	2.5263	2.3008	2.1947	2.6823	2.2333	2.5245	2.5446
1.56...1.58	2.7283	2.4447	2.77	2.5606	2.4637	2.9535	2.4131	2.7841	2.6801
1.58...1.64	10.27	10.389	11.372	9.897	9.7957	10.602	10.597	10.887	10.072
1.64...1.68	6.5466	6.6422	7.3868	6.6314	6.5464	7.2661	6.598	7.2762	7.2588
1.68...1.74	6.9874	6.3902	7.4949	6.4259	6.3041	7.1663	6.4234	7.036	6.8515
1.74...1.78	9.2864	9.168	9.1563	8.7273	8.7432	9.1665	8.8483	9.0645	8.8411
1.78...1.83	23.137	24.341	25.331	22.299	22.48	22.343	23.268	23.691	21.526
1.83...1.87	6.0286	6.3831	6.658	5.5018	5.5193	6.0809	6.3671	6.6684	6.5276
1.87...1.93	8.2297	8.139	9.9947	7.8229	7.766	8.5468	7.9718	8.2293	8.4922
1.93...1.95	1.8191	1.7551	2.1629	1.8797	1.8305	2.1609	1.7374	2.1755	2.1868
1.95...1.98	2.7027	2.6709	3.0967	2.8436	2.8052	3.1216	2.3853	2.785	2.6203
1.98...2.01	5.0193	4.886	5.4095	5.1287	5.1003	5.3792	4.6186	5.1633	4.9394
2.01...2.07	15.542	15.533	17.469	15.811	15.899	16.1	15.596	15.787	15.56
2.07...2.11	6.2991	6.0808	7.1647	6.49	6.4693	6.9901	5.9132	6.8499	6.4258
2.11...2.15	4.5044	3.9138	4.9347	4.2409	4.1239	4.604	3.8525	4.2428	4.1963
2.15...2.18	5.1388	4.3115	5.837	4.9709	4.9669	5.145	3.0977	3.1728	3.0416
2.18...2.24	33.217	35.262	36.428	32.342	32.816	31.942	36.132	35.33	31.574
2.24...2.26	5.3641	6.2969	6.0336	5.3537	5.4222	5.5301	6.2671	6.163	6.7188
2.26...2.30	6.6142	6.4913	6.4032	6.1068	6.1655	6.4617	5.4872	6.5067	5.7392
2.30...2.34	5.1938	4.9129	4.824	4.6699	4.6873	5.0101	3.9533	4.6419	4.4822
2.34...2.40	9.5961	8.3332	9.4538	9.2975	9.3854	9.6654	8.6788	9.6147	7.0828
2.40...2.42	2.3618	2.2226	2.3412	2.5162	2.5329	2.7304	2.335	2.5922	1.9693
2.42...2.48	5.4747	5.0931	5.6888	5.8608	5.8497	6.3755	5.2973	5.6179	6.0625
2.48...2.50	1.4234	1.5402	1.6634	1.529	1.4864	1.749	1.8065	1.6632	1.6986
2.50...2.52	1.3249	1.4167	1.4474	1.4476	1.3983	1.6253	1.5158	1.3682	1.5606
2.52...2.58	8.6076	7.3907	7.365	14.511	14.673	14.613	7.6875	8.0978	7.2698
2.58...2.63	4.0459	3.4008	3.8112	3.5957	3.5659	3.9312	3.538	4.3085	4.1833
2.63...2.66	2.0728	1.7391	1.7543	1.9799	1.9315	2.2465	1.9014	1.8333	2.1208
2.66...2.68	1.0447	0.90611	1.111	1.2366	1.1531	1.3892	0.88654	1.0051	1.0418
2.68...2.70	3.4345	3.0494	3.0011	7.0899	7.1802	7.0583	3.0284	3.2047	2.5513
2.70...2.73	5.6541	5.1101	5.3455	7.4434	7.59	7.3872	5.5307	5.3752	4.5902
2.73...2.78	14.082	15.377	15.477	13.393	13.586	13.478	14.2	14.429	12.003
2.78...2.84	2.0895	1.8624	2.0807	1.6945	1.6287	2.1114	2.0107	2.4069	2.5456
2.84...2.86	0.73242	0.59902	0.71675	0.59326	0.54987	0.75149	0.58442	0.79661	0.84437
2.86...2.90	18.412	14.532	15.894	10.583	10.737	10.509	17.574	19.165	12.517
2.90...2.95	4.8041	5.4849	4.6244	4.5873	4.6362	4.817	4.9654	4.5031	4.2401
2.95...2.97	0.87843	0.85915	0.82935	0.83745	0.78581	0.945	0.62621	0.89709	0.99518
2.97...3.01	6.645	6.153	6.6456	7.9339	7.8913	7.944	5.6646	6.4898	5.9455
3.01...3.06	14.041	15.484	15.061	15.615	15.851	15.303	21.173	14.825	18.644
3.06...3.11	5.5574	5.2786	5.4993	5.0799	5.017	5.1951	5.5623	5.5697	5.8636
3.11...3.13	2.2304	2.1192	2.3235	2.1841	2.1416	2.2249	2.3863	2.4832	2.6987
3.13...3.19	10.93	10.901	10.957	11.894	11.885	11.712	11.061	11.115	12.067
3.19...3.22	3.4069	2.9646	3.0062	3.09	2.983	3.0508	2.8864	2.986	3.5664
3.22...3.24	5.382	4.5638	4.4962	4.787	4.5863	4.6163	3.7047	3.5275	3.357
3.24...3.30	144.08	124	124.04	93.355	95.464	90.01	139.79	111.72	123.92
3.30...3.33	3.5175	3.3734	3.8289	3.1453	3.2625	3.2109	4.5883	4.5039	4.0997
3.33...3.37	4.0505	3.9931	3.8292	3.6812	3.7015	3.7197	4.2531	3.7886	4.2634
3.37...3.39	1.7201	1.9398	1.616	1.7979	1.7686	1.7859	1.9709	1.8017	1.9303
3.39...3.45	60.164	48.89	33.605	42.157	43.025	40.671	51.967	42.363	44.451
3.45...3.48	6.0146	7.4368	6.6098	8.3795	8.5418	8.147	6.4159	7.0502	6.8122
3.48...3.51	5.9145	6.4398	6.4736	6.784	6.9137	6.6039	6.1244	6.1676	6.2075
3.51...3.53	4.2783	4.3274	4.2803	4.1891	4.2444	4.0731	4.4721	3.9292	3.9433
3.53...3.57	15.724	18.411	17.664	21.22	21.569	20.46	16.426	17.989	18.152
3.57...3.60	7.3359	10.771	7.0696	8.5806	8.7535	8.299	7.8552	9.3224	8.6638
3.60...3.62	11.993	13.813	11.45	11.734	11.95	11.341	12.586	12.428	12.435
3.62...3.66	16.541	19.844	17.294	17.642	17.95	16.987	17.94	18.511	18.333
3.66...3.72	35.899	42.481	37.459	46.14	47.06	44.38	37.228	40.998	39.259
3.72...3.77	30.502	35.46	30.588	32.649	33.393	31.468	30.89	33.006	30.635
3.77...3.79	12.505	15.41	14.289	16.337	16.655	15.703	14.566	15.785	14.78
3.79...3.85	33.986	44.362	35.983	49.94	51.122	48.044	37.285	42.657	40.011
3.85...3.91	24.649	30.396	27.1	31.051	31.873	29.887	27.485	30.035	28.41
3.91...3.97	17.574	22.915	20.227	21.121	21.811	20.352	24.732	20.778	22.919
3.97...4.00	7.6609	8.4129	9.2032	10.673	10.967	10.236	8.6833	8.863	8.612
4.00...4.04	11.69	13.005	13.535	15.286	15.702	14.66	13.075	12.974	11.777
4.04...4.08	10.466	12.016	11.973	13.282	13.753	12.784	11.8	12.514	12.003
4.08...4.14	13.707	14.369	15.695	15.272	15.813	14.607	15.197	15.174	14.891
4.14...4.18	4.3618	4.9474	5.4492	4.8861	5.1142	4.617	5.2085	5.2337	4.9697
4.18...4.23	4.465	6.4002	4.8978	7.3622	7.6393	6.9624	5.4048	6.4216	5.7727
4.23...4.25	1.4567	1.8821	1.6618	1.8308	1.9418	1.719	1.8399	1.802	1.7093
4.25...4.30	4.4997	5.3384	5.311	5.5824	5.816	5.1644	5.2298	5.6912	4.9351
4.30...4.36	28.654	35.18	38.218	31.007	32.344	29.731	36.215	31.07	38.86
4.36...4.42	2.2933	3.4606	3.2284	3.3317	3.6061	2.992	3.3943	3.5995	3.4204
4.42...4.48	2.6121	3.6853	3.6085	3.69	3.9931	3.3027	3.8571	3.7707	3.6614
4.48...4.53	1.366	1.9175	1.8516	1.8674	2.0434	1.5521	2.0222	2.0859	1.8371
4.53...4.59	1.7373	2.3028	2.2422	2.3464	2.5525	1.9397	2.5787	2.4342	2.2548
4.59...4.61	0.28604	0.59253	0.42828	0.59264	0.66922	0.44568	0.62676	0.61534	0.41792
4.61...4.67	2.0522	3.2136	2.435	2.9623	3.1672	2.4704	2.3334	2.8027	2.2205
4.67...4.70	0	0.29167	0.1124	0.28253	0.36961	0.059109	0.32817	0.36155	0.17232
4.90...4.94	0.11171	0.29427	0.087128	0.451	0.37352	0.055211	0.34986	0.34056	0.32978
4.94...4.99	0	0.33277	0	0.47019	0.33321	0.04094	0.2658	0.42118	0.37594
4.99...5.03	0.051604	0.09306	0.042696	0.31432	0.19364	0	0.20261	0.25793	0.31852
5.03...5.05	0	0	0	0.12861	0.048439	0	0.034314	0.065998	0.10172
5.05...5.10	0.33095	0.27567	0.11378	0.50489	0.2518	0.082769	0.20683	0.29667	0.45671
5.10...5.15	0.13026	0.078436	0.07952	0.43663	0.14312	0.031642	0.24053	0.22137	0.41509
5.15...5.17	0	0	0	0.14577	0	0	0.018368	0.037989	0.13502
5.17...5.22	0.45826	0.27431	0.47355	0.71086	0.41934	0.38387	0.41105	0.40975	0.60769
5.22...5.25	1.8724	2.7348	2.0862	2.5334	2.3226	2.1992	1.7551	2.3797	2.223
5.25...5.31	0.06481	0.0064973	0.097443	0.37552	0	0.034873	0.042993	0.14884	0.45089
5.31...5.34	0.034542	0.1597	0.037154	0.24707	0	0.055982	0.073082	0.17289	0.30272
5.34...5.36	0.017911	0.11989	0	0.16835	0	0.041718	0	0.1029	0.15975
5.36...5.42	12.861	14.538	12.243	14.919	14.785	14.033	14.017	14.407	12.744
5.42...5.47	0.7704	0.66783	0.78035	1.0832	0.55037	0.78241	0.96421	0.83276	1.1574
5.47...5.50	0.25295	0.054581	0.22684	0.3596	0.057322	0.20896	0.20689	0.1135	0.29585
5.90...5.96	3.9465	1.8223	4.3521	3.8774	3.8165	3.761	2.817	2.2312	2.3138
5.96...5.99	1.0993	0	1.1771	1.0443	0.99442	1.0197	0.54367	0.33086	0.38039
5.99...6.01	0.77117	0.084451	0.66862	0.64391	0.6076	0.64158	0.37607	0.20376	0.20628
6.01...6.07	6.6081	3.4062	5.9349	5.4804	5.4708	5.3066	5.1005	4.0058	3.2476
6.07...6.13	0.85708	0	0.95203	0.73007	0.67082	0.75083	0.48432	0.34728	0.47415
6.13...6.17	0.30858	0	0.32083	0.18029	0.18503	0.24973	0.13008	0.071852	0.11276
6.17...6.22	0.26973	0	0.27329	0.12004	0.14017	0.20495	0.12237	0.087664	0.12092
6.22...6.27	0.12079	0.012913	0.11272	0.0031982	0.0035495	0.058344	0.005418	0.0055431	0.0095828
6.27...6.32	0.18762	0.14328	0.18732	0.09556	0.081544	0.089968	0.10479	0.10011	0.063975
6.32...6.37	0	0	0	0	0	0	0	0	0
6.37...6.39	0	0	0	0	0	0	0.0015794	0	0
6.39...6.41	0.048898	0.0078119	0.05448	0.036924	0.057638	0.01283	0	0.0011519	0
6.41...6.46	0.09182	0.14341	0.077778	0.043896	0.067512	0.026268	0.15672	0.16756	0.18042
6.46...6.49	0.0164	0.00072562	0	0.0001565	0	0	0.016112	0.035856	0.033004
6.49...6.53	0	0	0	0	0.0083387	0	0	0.0096615	0
6.53...6.57	0	0.0066036	0.0048491	0.0078084	0	0	0.010899	0.0013453	0
6.57...6.59	0	0	0	0.0016857	0	0	0	0	0
6.59...6.64	0.0062175	0.1172	0.15598	0.17058	0.091095	0.055486	0.15332	0.17416	0.16757
6.64...6.70	0.25724	0.30324	0.3699	0.43955	0.25215	0.32986	0.42279	0.41281	0.38499
6.70...6.74	0.022937	0.0033987	0.0090798	0.088103	0	0.013727	0.035935	0.086496	0.096905
6.74...6.76	0.022678	0	0.023001	0.065344	0.0054172	0.020312	0.056727	0.070303	0.076305
6.76...6.78	0.079084	0.04312	0.083176	0.13869	0.082431	0.088946	0.10195	0.14279	0.13247
6.78...6.80	0.12886	0.11239	0.093818	0.18205	0.12361	0.13197	0.11541	0.16823	0.13151
6.80...6.83	0.44943	0.50024	0.50042	0.61044	0.53287	0.5296	0.44943	0.58373	0.46417
6.83...6.87	0.99839	0.97803	1.0651	1.1989	1.0892	1.0665	0.98757	1.142	0.98049
6.87...6.91	1.8152	1.8007	1.9177	1.9748	1.8612	1.8209	1.7425	1.9052	1.6858
6.91...6.97	0.72937	0.45984	1.0696	0.90162	0.70167	0.74528	0.84964	0.87977	1.0703
6.97...7.00	0.24419	0.0059279	0.22678	0.3022	0.18515	0.23548	0.24445	0.26612	0.31099
7.00...7.05	1.1564	0.59339	0.69329	0.81529	0.60336	0.69054	0.60706	0.73423	0.9639
7.05...7.07	0.13158	0	0.070536	0.1463	0.056021	0.093698	0.10943	0.13181	0.20237
7.07...7.09	0.081063	0	0.05122	0.14145	0.062632	0.10147	0.10536	0.17481	0.23485
7.09...7.11	0.2603	0.19923	0.22269	0.38464	0.30157	0.33037	0.29448	0.37008	0.37573
7.11...7.17	2.5679	2.2345	2.3232	2.5706	2.4078	2.3917	2.4001	2.436	2.0774
7.17...7.22	4.1101	2.7709	2.7736	2.8453	2.7037	2.6788	2.4295	2.4237	2.6449
7.22...7.24	0.5594	0.49291	0.41434	0.58229	0.51963	0.53507	0.61573	0.6824	0.55689
7.24...7.28	5.1932	4.497	4.4067	4.0414	3.9821	3.8225	3.849	4.2255	4.481
7.28...7.33	5.3535	5.3043	5.3588	5.032	4.9138	4.7665	6.1365	5.4241	5.5486
7.33...7.39	6.3862	4.8664	4.8824	4.9068	4.7155	4.6257	4.9065	5.1927	5.54
7.39...7.42	2.5938	1.9138	1.8841	1.8829	1.814	1.7722	1.6886	1.9976	2.0914
7.42...7.42	0.79057	0.46069	0.57823	0.55066	0.55683	0.52652	0.37853	0.49936	0.54112
7.47...7.51	2.1033	2.1113	1.7742	1.6424	1.5744	1.6023	2.389	1.6355	2.5707
7.51...7.57	1.7452	1.8887	1.7903	1.8932	1.6908	1.8089	1.7018	1.618	2.1702
7.57...7.61	0.55254	0.5994	0.51083	0.74134	0.59149	0.70517	0.55807	0.4623	0.7794
7.61...7.65	0.97301	0.98931	0.9671	1.1137	0.91937	1.0594	0.97343	0.91675	1.3173
7.65...7.71	1.869	1.2363	1.4883	1.5092	1.279	1.4374	1.5086	1.2536	1.6629
7.71...7.77	0.36303	0.1434	0.30514	0.60589	0.30284	0.57662	0.29758	0.28209	0.68118
7.77...7.79	0.014307	0	0	0.10133	0	0.089962	0	0	0.14013
7.79...7.84	1.1453	1.0377	1.1937	1.3082	0.99969	1.2393	1.0999	1.0629	1.5715
7.84...7.88	0.35804	0.02363	0.31969	0.5151	0.28682	0.47828	0.27483	0.25681	0.59216
7.88...7.91	0.18962	0	0.17508	0.32504	0.14852	0.3136	0.16831	0.17881	0.42372
7.91...7.94	0.29496	0.021715	0.22099	0.4071	0.26017	0.38103	0.23558	0.27025	0.40887
7.94...7.96	0.37316	0.19534	0.34475	0.47866	0.35161	0.46581	0.35563	0.31692	0.4678
7.96...8.02	1.771	1.1281	1.5512	1.861	1.5454	1.7927	1.391	1.5157	1.958
8.02...8.08	1.0156	0.47904	1.0523	1.2944	0.95216	1.2432	0.88024	0.9549	1.5324
8.08...8.12	0.34698	0.036834	0.29296	0.63156	0.32706	0.61459	0.16626	0.27577	0.63885
8.12...8.15	0.060465	0.077493	0.028454	0.25529	0.041969	0.2459	0.0028379	0.070804	0.3521
8.15...8.20	0.32196	0.21642	0.30073	0.61435	0.32158	0.61465	0.46998	0.44308	0.79659
8.20...8.23	0.02702	0	0.048209	0.25813	0.068932	0.25537	0	0.04629	0.26711
8.23...8.28	0.39432	0.21682	0.18493	0.73858	0.375	0.74734	0.35481	0.40466	0.98882
8.28...8.34	0.20304	0.28108	0.28692	0.75934	0.33533	0.74623	0.34748	0.31166	0.88079
8.34...8.38	0	0.016282	0	0.27508	0	0.28625	0.0011337	0.05039	0.3081
8.38...8.40	0	0	0	0.11138	0	0.12752	0	0	0.13767
8.40...8.45	0.11691	0.12856	0.15448	0.3126	0.099023	0.35138	0.10955	0.21925	0.4172
8.45...8.49	0.4338	0.26191	0.44154	0.56427	0.37665	0.58713	0.15739	0.19824	0.52651
8.49...8.55	0.53576	0.76362	0.50633	0.6523	0.47472	0.66358	0.71091	0.64979	0.98841
8.55...8.57	0.0013283	0.052601	0	0.045215	0	0.065611	0.11436	0.088513	0.37728
8.57...8.62	0.1987	0.27157	0.18949	0.22346	0.19925	0.28533	0.0013051	0.0011205	0.22083
8.62...8.68	0.10235	0.18692	0.1555	0.11818	0.097318	0.14564	0.38695	0.26592	0.51084
8.68...8.72	0	0	0	0	0.001019	0.037373	0	0.0065937	0.14056
8.72...8.78	0.017775	0.014252	0.037971	0.01348	0.011794	0.10062	0.01245	0.030336	0.18575
8.78...8.83	0.0034919	0.024216	0.010129	0.002092	0	0.039846	0.0033107	0.0087813	0.1543
8.83...8.87	0	0	0	0	0	0.020243	0	0.00013257	0.099256
8.87...8.92	0.11857	0.17017	0.18353	0.18252	0.15595	0.16862	0.17495	0.13243	0.33372
8.92...8.97	0.11917	0.16885	0.22444	0.18078	0.18452	0.17726	0.20182	0.12817	0.34595
8.97...9.00	0.0030957	0.0015581	0.0081637	0.0010847	0.00098593	0	0.011056	0.0056741	0.10313
NMR_BINNED_DATA_END
#END