#METABOLOMICS WORKBENCH araskind_20150415_9111471_mwtab.txt DATATRACK_ID:239 STUDY_ID:ST000162 ANALYSIS_ID:AN000254 PROJECT_ID:PR000135 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE pfk2 PR:PROJECT_SUMMARY Yeast glycolysis in normoxia and hypoxia PR:INSTITUTE University of Michigan PR:DEPARTMENT LSI & Human Genetics PR:LABORATORY Kim Lab PR:LAST_NAME Kim PR:FIRST_NAME John PR:ADDRESS Ann Arbor, MI PR:EMAIL jnkim@umich.edu PR:PHONE 734-615-9915 #STUDY ST:STUDY_TITLE Yeast glycolysis in normoxia and hypoxia (150121_pkf2) ST:STUDY_TYPE Yeast glycolysis ST:STUDY_SUMMARY Analyze yeast glycolysis ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 12 ST:TOTAL_SUBJECTS 12 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Saccharomyces cerevisiae SU:TAXONOMY_ID 4932 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011634 S00017179 strain:aro1D | experiment:1 | O2:- SUBJECT_SAMPLE_FACTORS SU0011631 S00017176 strain:aro1D | experiment:1 | O2:+ SUBJECT_SAMPLE_FACTORS SU0011640 S00017185 strain:aro1D | experiment:2 | O2:- SUBJECT_SAMPLE_FACTORS SU0011637 S00017182 strain:aro1D | experiment:2 | O2:+ SUBJECT_SAMPLE_FACTORS SU0011635 S00017180 strain:atp1D | experiment:1 | O2:- SUBJECT_SAMPLE_FACTORS SU0011632 S00017177 strain:atp1D | experiment:1 | O2:+ SUBJECT_SAMPLE_FACTORS SU0011641 S00017186 strain:atp1D | experiment:2 | O2:- SUBJECT_SAMPLE_FACTORS SU0011638 S00017183 strain:atp1D | experiment:2 | O2:+ SUBJECT_SAMPLE_FACTORS SU0011633 S00017178 strain:WT | experiment:1 | O2:- SUBJECT_SAMPLE_FACTORS SU0011630 S00017175 strain:WT | experiment:1 | O2:+ SUBJECT_SAMPLE_FACTORS SU0011639 S00017184 strain:WT | experiment:2 | O2:- SUBJECT_SAMPLE_FACTORS SU0011636 S00017181 strain:WT | experiment:2 | O2:+ #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Saccharomyces cerevisiae #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) CH:METHODS_ID AQM020 CH:METHODS_FILENAME QTOF-002-HILIC-35min-1mm.m #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE QTOF-002-HILIC-35min-1mm.m AN:PROCESSING_PARAMETERS_FILE EX00391-Quant-method.m #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS mM MS_METABOLITE_DATA_START Samples S00017179 S00017176 S00017185 S00017182 S00017180 S00017177 S00017186 S00017183 S00017178 S00017175 S00017184 S00017181 Factors strain:aro1D | experiment:1 | O2:- strain:aro1D | experiment:1 | O2:+ strain:aro1D | experiment:2 | O2:- strain:aro1D | experiment:2 | O2:+ strain:atp1D | experiment:1 | O2:- strain:atp1D | experiment:1 | O2:+ strain:atp1D | experiment:2 | O2:- strain:atp1D | experiment:2 | O2:+ strain:WT | experiment:1 | O2:- strain:WT | experiment:1 | O2:+ strain:WT | experiment:2 | O2:- strain:WT | experiment:2 | O2:+ 2 or 3-phosphoglycerate_2PG/3PG 0.1544 1.7477 2.8034 1.2364 4.0694 1.5487 5.2699 0.6435 7.4499 2.5540 0.9421 2.6120 6-Phosphogluconate 0.6246 0.1619 1.2463 0.2053 1.9981 0.2224 2.2449 0.1798 5.6814 0.0985 0.5578 0.1760 Acetyl-CoA 0.7686 0.4353 2.2487 0.6147 2.4652 0.4786 3.0821 0.4858 1.5324 0.6832 1.0557 1.1734 ADP 6.3859 7.6427 8.1381 11.7500 6.5128 9.1975 2.2725 9.6386 6.4954 8.9502 6.6619 12.8729 AMP 112.7884 93.0152 123.7470 126.4985 144.5240 262.9099 90.2970 95.7883 120.5452 98.8821 106.0650 100.6215 ATP 15.9979 22.4267 39.3829 19.5973 44.2646 3.7158 29.7515 9.7227 80.9195 13.4050 28.0752 19.5290 Citrate or Isocitrate 101.9188 50.8878 140.3746 30.1478 565.1276 78.1994 500.2815 103.9081 184.1240 48.5644 160.2683 35.6104 Erythrose 4-phosphate 11.4012 5.8098 24.6243 8.0994 19.3597 3.8407 15.5355 5.7633 14.3200 5.8697 15.7112 12.6047 Fructose 1_6-bisphosphate 22.1510 5.5354 44.8482 2.7652 73.8825 14.0902 66.2748 13.7571 122.6726 3.6431 48.2933 2.4823 Malate 65.0324 128.4433 92.2348 49.7296 269.2597 237.3845 232.4966 230.9256 61.1336 93.4489 40.9488 53.7743 Nicotinamide Adenine Dinucleotide oxidized 3.5909 0.7981 10.0308 14.9263 6.1942 11.2955 3.6267 14.6562 2.8506 12.3633 10.3468 18.5041 Nicotinamide Adenine Dinucleotide reduced 0.1673 0.1444 0.2165 0.0401 0.1948 0.3098 0.1572 0.8439 0.7296 0.0921 0.9768 0.0632 Phosphoenolpyruvate 0.3246 0.7135 0.3771 0.4510 0.3412 1.0488 Ribose-5-phosphate or xylulose 5-phosphate 0.7022 0.2535 2.6518 0.4066 3.0553 0.2306 Sedoheptulose 7-phosphate 4.0835 2.3267 0.7532 0.5937 8.1575 1.9068 0.6758 0.9001 6.5814 1.8265 0.0000 1.2442 Sucrose 277.3819 281.4627 2.7178 0.2716 266.1213 323.3222 2.1028 0.9321 286.0324 190.0721 2.8258 0.2774 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type 2 or 3-phosphoglycerate_2PG/3PG 724 2PG/3PG UM_Target_ID 6-Phosphogluconate 91493 6PG UM_Target_ID Acetyl-CoA 444493 aCoA UM_Target_ID ADP 6022 ADP UM_Target_ID AMP 6083 AMP UM_Target_ID ATP 5957 ATP UM_Target_ID Citrate or Isocitrate 311 CIT/ICIT UM_Target_ID Erythrose 4-phosphate 122357 E4P UM_Target_ID Fructose 1,6-bisphosphate 21125049 FBP UM_Target_ID Malate 525 MAL UM_Target_ID Nicotinamide Adenine Dinucleotide oxidized 15938971 NAD UM_Target_ID Nicotinamide Adenine Dinucleotide reduced 439153 NADH UM_Target_ID Phosphoenolpyruvate 1005 PEP UM_Target_ID Ribose-5-phosphate or xylulose 5-phosphate 77982 R5P/X5P UM_Target_ID Sedoheptulose 7-phosphate 165007 S7P UM_Target_ID Sucrose 5988 SUC UM_Target_ID METABOLITES_END #END