#METABOLOMICS WORKBENCH rgn5011_20150618_9618511_mwtab.txt DATATRACK_ID:327 TEXT OUTPUT STUDY_ID:ST000226 ANALYSIS_ID:AN000336 PROJECT_ID:PR000120
VERSION             	1
CREATED_ON          	10-02-2015
#PROJECT
PR:PROJECT_TITLE                 	Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts
PR:PROJECT_TITLE                 	Gut Microbiota-Host Metabolic Homeostasis in Mice
PR:INSTITUTE                     	Pennsylvania State University
PR:DEPARTMENT                    	Molecular Toxicology
PR:LAST_NAME                     	Andrew
PR:FIRST_NAME                    	Patterson
PR:EMAIL                         	adp117@PSU.EDU, RGN5011@psu.edu
PR:PROJECT_SUMMARY               	-
PR:PHONE                         	-
PR:ADDRESS                       	-
#STUDY
ST:STUDY_TITLE                   	Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental
ST:STUDY_TITLE                   	Chemicals Induces Systemic Metabolic Dysfunction in Mice (Part I)
ST:STUDY_TYPE                    	drug dosage
ST:STUDY_SUMMARY                 	12 mice were acclimatized for a week, taught to eat dough pills then half were
ST:STUDY_SUMMARY                 	treated with TCDF per pill per day for 5 days
ST:STUDY_SUMMARY                 	Also 10 AHR -/- mice were used and treated the same way
ST:INSTITUTE                     	Pennsylvania State University
ST:DEPARTMENT                    	Molecular Toxicology
ST:LAST_NAME                     	Patterson
ST:FIRST_NAME                    	Andrew
ST:EMAIL                         	adp117@PSU.EDU
ST:PHONE                         	-
ST:ADDRESS                       	-
#SUBJECT
SU:SUBJECT_TYPE                  	mouse
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6J; Wild Type and Ahr-/-
SU:AGE_OR_AGE_RANGE              	6 weeks old
SU:GENDER                        	Males
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Treated serum_Ahr-	Gender:Male | Genotype:Ahr -/- | Treatment:TCDF	
SUBJECT_SAMPLE_FACTORS           	-	Treated Liver	Gender:Male | Genotype:Ahr +/+ | Treatment:TCDF	
SUBJECT_SAMPLE_FACTORS           	-	Treated serum_Ahr+	Gender:Male | Genotype:Ahr +/+ | Treatment:TCDF	
SUBJECT_SAMPLE_FACTORS           	-	Control serum_Ahr-	Gender:Male | Genotype:Ahr -/- | Treatment:Vehicle	
SUBJECT_SAMPLE_FACTORS           	-	Control Liver	Gender:Male | Genotype:Ahr +/+ | Treatment:Vehicle	
SUBJECT_SAMPLE_FACTORS           	-	Control serum_Ahr+	Gender:Male | Genotype:Ahr +/+ | Treatment:Vehicle	
#COLLECTION
CO:SAMPLE_TYPE                   	Feces, liver, cecal contents, intestine and blood (only liver and blood tested
CO:SAMPLE_TYPE                   	with NRM)
CO:COLLECTION_SUMMARY            	-
#TREATMENT
TR:TREATMENT_SUMMARY             	Wild type and AHR knock out mice (12 wild types and 10 AHR knock out) were
TR:TREATMENT_SUMMARY             	treated with TCDF for 5 days
TR:TREATMENT                     	Dietary
TR:TREATMENT_COMPOUND            	TCDF
TR:TREATMENT_ROUTE               	Oral
TR:TREATMENT_DOSE                	24 µg/kg body weight for AHR and 5 µg/kg body weight  for wild type
#SAMPLEPREP
SP:SAMPLEPREP_PROTOCOL_FILENAME  	NMR_metabolomics_protocol.docx
SP:PROCESSING_METHOD             	Homogenization
SP:PROCESSING_STORAGE_CONDITIONS 	-20
SP:EXTRACTION_METHOD             	Methanol/Water
SP:SAMPLE_RESUSPENSION           	D2O
SP:SAMPLE_SPIKING                	TSP-d4
SP:ORGAN                         	Liver, small and large intestine
SP:SAMPLEPREP_SUMMARY            	-
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	-
CH:COLUMN_NAME                   	-
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
#NMR
NM:INSTRUMENT_TYPE               	Bruker Avance III 600 MHz spectrometer
NM:NMR_EXPERIMENT_TYPE           	NOESY PR1D
NM:SPECTROMETER_FREQUENCY        	600 MHz
NM:NMR_SOLVENT                   	D2O
NM:INSTRUMENT_NAME               	Bruker Avance III
#END