#METABOLOMICS WORKBENCH michaelsa93_20151124_9180641_mwtab.txt DATATRACK_ID:436 efahy_20160115_125346 STUDY_ID:ST000322 ANALYSIS_ID:AN000513 PROJECT_ID:PR000261 VERSION 1 CREATED_ON January 15, 2016, 12:53 pm #PROJECT PR:PROJECT_TITLE Progesterone level effects on primary metabolites in uterus, blood, and ovaries PR:PROJECT_SUMMARY In this experiment a hormonal protocol was applied to control follicle growth to PR:PROJECT_SUMMARY yield larger or smaller preovulatory follicle and CLs and consequently different PR:PROJECT_SUMMARY circulating Progesterone (P4) concentrations during early diestrus. The two PR:PROJECT_SUMMARY different animal's group are: high or low progesterone levels. The effects of PR:PROJECT_SUMMARY these progesterone levels was tested in the uterus, blood, and ovaries of the PR:PROJECT_SUMMARY cow. PR:INSTITUTE University of California, Davis PR:DEPARTMENT Genome and Biomedical Sciences Facility PR:LABORATORY WCMC Metabolomics Core PR:LAST_NAME Fiehn PR:FIRST_NAME Oliver PR:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, PR:ADDRESS CA 95616 PR:EMAIL ofiehn@ucdavis.edu PR:PHONE (530) 754-8258 PR:FUNDING_SOURCE U24DK097154 #STUDY ST:STUDY_TITLE Progesterone level effects on primary metabolites in uterus, blood, and ovaries ST:STUDY_TITLE (Part 1:Plasma) ST:STUDY_SUMMARY In this experiment a hormonal protocol was applied to control follicle growth to ST:STUDY_SUMMARY yield larger or smaller preovulatory follicle and CLs and consequently different ST:STUDY_SUMMARY circulating Progesterone (P4) concentrations during early diestrus. The two ST:STUDY_SUMMARY different animal's group are: high or low progesterone levels. The effects of ST:STUDY_SUMMARY these progesterone levels was tested in the blood of the cow. ST:INSTITUTE University of California, Davis ST:DEPARTMENT Genome and Biomedical Sciences Facility ST:LABORATORY WCMC Metabolomics Core ST:LAST_NAME Fiehn ST:FIRST_NAME Oliver ST:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, ST:ADDRESS CA 95616 ST:EMAIL ofiehn@ucdavis.edu ST:PHONE (530) 754-8258 ST:NUM_GROUPS 1 ST:TOTAL_SUBJECTS 80 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Bos taurus #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 1 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 2 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 3 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 4 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 5 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 6 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 7 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 8 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 9 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 10 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 11 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 12 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 13 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 14 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 15 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 16 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 17 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 18 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 19 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 20 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 21 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 22 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 23 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 24 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 25 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 26 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 27 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 28 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 29 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 30 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 31 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 32 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 33 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 34 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 35 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 36 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 37 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 38 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 39 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 40 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 41 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 42 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 43 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 44 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 45 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 46 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 47 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 48 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 49 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 50 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 51 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 52 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 53 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 54 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 55 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 56 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 57 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 58 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 59 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 60 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 101 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 102 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 103 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 104 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 105 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 106 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 107 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 108 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 109 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 110 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 151 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 152 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 153 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 154 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 155 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 156 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 157 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 158 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 159 Organ:Plasma SUBJECT_SAMPLE_FACTORS - 160 Organ:Plasma #COLLECTION CO:COLLECTION_SUMMARY Plasma: plasma were obtained by centrifugation of the blood collected from cows CO:COLLECTION_SUMMARY and kept at 80ºC. There was no inactivation or attenuation because samples are CO:COLLECTION_SUMMARY not biohazard. CO:COLLECTION_PROTOCOL_FILENAME StudyDesign-MarioBinelli-101414.pdf CO:SAMPLE_TYPE Plasma #TREATMENT TR:TREATMENT_SUMMARY There was no specific treatment. A hormonal protocol was applied to control TR:TREATMENT_SUMMARY follicle growth to yield larger or smaller preovulatory follicle and CLs and TR:TREATMENT_SUMMARY consequently different circulating Progesterone (P4) concentrations during early TR:TREATMENT_SUMMARY diestrus. The two different animal's group are: high or low progesterone levels. TR:TREATMENT_PROTOCOL_FILENAME StudyDesign-MarioBinelli-101414.pdf #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME SOP_Extraction_of_Blood_Samples.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY UPLC CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Unspecified CH:COLUMN_NAME Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) CH:COLUMN_NAME 1.7um Pre-Column CH:FLOW_GRADIENT 15% B to 99% B CH:FLOW_RATE 0.6 mL/min CH:COLUMN_TEMPERATURE 65 C CH:METHODS_FILENAME Data Dictionary Fiehn laboratory_CSH QTOF lipidomics_05-29-2014.pdf CH:SOLVENT_A 60:40 Acetonitrile:Water +10mM Ammonium Formate +10mM Formic Acid CH:SOLVENT_B 9:1 Isopropanol:Acetonitrile +10mM Ammonium Formate +10mM Formic Acid CH:COLUMN_PRESSURE 450-850 bar CH:INTERNAL_STANDARD See data dictionary CH:RETENTION_TIME See data dictionary CH:SAMPLE_INJECTION 1.67 uL CH:ANALYTICAL_TIME 13 min CH:CAPILLARY_VOLTAGE 3500 CH:TIME_PROGRAM 15 min CH:WEAK_WASH_SOLVENT_NAME Isopropanol CH:WEAK_WASH_VOLUME 5 seconds CH:STRONG_WASH_SOLVENT_NAME Isopropanol CH:TARGET_SAMPLE_TEMPERATURE Autosampler temp 4 C CH:RANDOMIZATION_ORDER Excel #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME WCMC Metabolomics Core AN:DETECTOR_TYPE TOF MCP AN:SOFTWARE_VERSION MassHunter AN:DATA_FORMAT .d #MS MS:INSTRUMENT_NAME Agilent 6550 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs MS:CAPILLARY_VOLTAGE 3500 MS:COLLISION_GAS Nitrogen MS:DRY_GAS_FLOW 8 l/min MS:DRY_GAS_TEMP 325 MS:FRAGMENT_VOLTAGE 120 MS:FRAGMENTATION_METHOD Auto MS/MS MS:ION_SOURCE_TEMPERATURE 325 MS:ION_SPRAY_VOLTAGE 1000 MS:IONIZATION Pos MS:PRECURSOR_TYPE Intact Molecule MS:REAGENT_GAS Nitrogen MS:SOURCE_TEMPERATURE 325 C MS:DESOLVATION_GAS_FLOW 11 l/min MS:DESOLVATION_TEMPERATURE 350 C MS:NEBULIZER 35 psig MS:OCTPOLE_VOLTAGE 750 MS:RESOLUTION_SETTING Extended Dynamic Range MS:SCAN_RANGE_MOVERZ 60-1700 Da MS:SCANNING_CYCLE 2 Hz MS:SCANNING_RANGE 60-1700 Da MS:SKIMMER_VOLTAGE 65 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak height MS_METABOLITE_DATA_START Samples 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 101 102 103 104 105 106 107 108 109 110 151 152 153 154 155 156 157 158 159 160 Factors Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Organ:Plasma Ceramide (d34:1) 3146 3701 4155 4376 2965 3020 2781 3630 4166 2790 4356 4846 4186 3301 3855 3217 4076 4776 2937 4211 4270 5179 4102 2510 3903 3599 3074 4733 2665 3131 4656 4679 5873 2976 4132 3483 4007 2649 2919 3569 4898 5248 3835 3702 3870 3253 4249 4130 3181 3165 4394 5610 6600 6523 4778 5952 3719 3473 3585 4683 4819 4943 5786 4103 4313 5712 3354 3779 4744 4020 4266 6324 3158 6494 3359 4521 4443 6385 4620 FA (16:1) (palmitoleic acid) 7939 6664 3855 4944 5443 5115 9844 4518 13495 6700 7067 10374 4922 14509 6404 13390 11079 8264 12240 10366 7940 9560 6710 6365 5650 10765 5772 3844 10563 6354 5716 9822 4588 8319 10777 6223 8278 8100 9459 6924 6629 10646 3855 8442 6325 9570 8696 6054 19957 8580 7375 7052 17292 21210 27770 39564 19905 17035 27337 30029 9830 12793 75704 10220 12153 9118 34913 8318 13366 55007 7099 8085 17647 3148 19294 7195 13741 8460 70042 8215 FA (17:1) (heptadecenoic acid) 4147 2809 2333 2506 2916 2455 4620 2632 4873 3153 3262 3831 6421 2445 3784 4735 2381 4338 3758 3667 3972 2686 3046 2599 4981 2438 3998 3147 2494 3945 3822 4125 2871 3364 2925 2665 2787 3100 4696 4374 2645 4503 3677 2349 6690 3531 3401 3345 4785 9695 12212 15505 10120 8427 10375 11204 4896 5443 18932 4192 4198 4788 9600 3958 3731 11126 3022 3295 6410 7942 3427 4430 3159 18242 3909 FA (18:1) (oleic acid) 249189 169767 111867 142097 113540 113174 370508 144345 343142 182001 178816 275976 81310 406281 78456 154713 360863 73423 280245 234763 208102 273037 146466 175170 84084 277417 173371 88772 309895 165838 117352 308100 94835 218971 154816 121814 231276 166396 157528 142189 203664 289477 69467 266626 103771 211319 265285 116080 520565 225002 211848 194655 289090 572008 688658 954052 563821 518146 688163 727184 286575 374754 1437027 241439 317876 352304 816599 223049 282186 797278 165047 199123 388338 71980 572223 205319 302846 173955 1269892 235437 FA (18:2) (linoleic acid) 22561 19517 16989 15347 27245 15314 31039 19204 23149 15676 20931 19398 15746 25358 19261 16299 24748 15197 21045 16130 25883 24001 20733 15883 19646 22129 21349 12374 22237 14233 16737 23497 16206 22186 27245 14756 20828 20662 15329 14397 18815 22901 12939 21323 22552 19796 18806 16723 30860 16563 35694 43969 51042 32212 33816 43572 29680 39658 42133 25509 72146 77819 132388 121797 127166 73181 88712 77116 30721 75462 30884 17241 50187 24801 37265 29615 23138 29640 62841 30317 FA (18:3) (linolenic acid) 14236 12560 9614 10197 21521 9707 16659 12217 11746 7025 11109 9433 6804 10705 13362 7247 12532 9743 9649 6397 13416 10881 10069 8346 13680 11541 11280 5505 9146 6855 9351 9806 7782 9594 16840 7724 8850 8811 5142 5839 8919 10404 6376 9302 13826 6725 8933 7153 11006 7943 5097 5230 6649 14979 15812 18467 14040 6142 8935 10095 44074 30809 37982 70585 34149 31381 29207 30603 14015 28319 4416 3346 7847 3365 6443 6231 5045 4926 12733 3172 FA (20:4) (arachidonic acid) 8385 7018 5949 5014 12837 6373 7770 6319 6894 4875 7545 6255 4480 5343 9064 5663 4939 5994 5141 4624 10602 7257 5895 4531 8904 7788 5686 5753 6048 5310 6604 6939 5999 6873 11700 7233 6359 7295 3287 4687 7135 7700 4403 4804 10672 5455 5438 4559 7035 5893 6813 8221 8516 11216 10178 10258 7626 7908 10191 8332 18075 14820 26608 29425 14428 19884 22650 18096 7382 21085 4703 4982 7316 3533 7236 6337 4425 6089 8204 3746 CSH_negESI #387 3255 5632 4104 3885 3814 5366 3505 3859 4616 3564 4962 3591 2825 3029 4817 2460 3680 4277 3356 2436 5057 3443 3352 3460 4281 2882 4000 4357 3308 2517 5098 4091 3889 3422 4653 2986 3396 2894 3213 3606 5775 3536 4008 2755 4532 3805 4251 4708 4283 3554 3981 4013 2045 3901 3513 2680 3350 3858 4934 3508 3029 5191 3433 3113 3203 2888 2950 2449 3219 3700 3894 3148 4082 2535 CSH_negESI #590 6346 22154 22375 26220 8984 17380 18951 22677 30269 11020 6270 22394 18481 15956 6308 16905 14820 19232 22249 13899 7161 23441 16852 19406 7492 15352 14901 14832 25709 14541 6091 22550 19743 22182 9263 17737 16583 16706 15945 14786 18581 22909 17911 19192 7269 15972 15341 16757 24003 19942 8994 8543 7167 9328 4756 5531 12211 5137 5534 6140 7007 9323 3519 13905 9162 4139 4392 3553 22866 2951 7760 3403 4607 10645 7858 9276 3370 7351 9772 5997 CSH_negESI #599 3157 4065 3429 3421 3052 3714 3427 3665 3488 2756 3375 3544 3106 2989 3820 3247 3850 3256 2763 3324 4037 3078 2846 2914 3669 3015 3373 3224 2708 2677 3510 3184 3537 3545 3779 3573 3512 2103 2810 3152 4428 3371 3348 3521 3854 2965 3348 3476 3315 3623 3684 3834 3546 4200 3244 4146 3324 4133 3602 3629 3436 3680 3858 3471 3469 4158 2907 3427 3715 3704 3184 3750 3662 3749 3730 3691 3984 3852 3570 CSH_negESI #644 13948 29303 26964 32563 19183 20680 26551 26184 30491 18242 12203 27328 22509 21829 15246 21050 20037 22870 24051 20617 13013 32136 22006 23180 14371 19565 21045 20031 26845 21655 11017 28209 23928 27445 17363 21638 21076 20356 17200 20291 21858 23791 22162 24136 16009 18983 20142 20193 23533 25092 21061 16539 18298 12084 7287 7662 13599 16717 16643 8881 17928 18803 12240 23149 17472 12921 15911 15327 25760 10388 20297 11599 13508 19161 16885 18169 12675 16076 16488 18550 CSH_negESI #743 6587 11808 12235 12032 8581 11400 9427 13016 12442 8138 6886 9933 9102 6709 7184 11549 6788 10914 9784 7468 8712 12074 9101 7469 7552 11757 8157 8536 11786 7508 5175 10206 8539 9211 9833 13823 7790 10146 5573 6829 6860 15161 8769 8391 8673 12112 7046 9848 10775 10024 11446 9407 11180 11020 10671 11217 13720 13645 8850 10274 11992 8974 11688 16021 10285 12218 15832 8338 11095 17241 10817 9005 10884 10765 9443 9472 8996 10177 11472 8504 0.50_187.01 20563 29866 8370 13812 7221 23398 28605 36740 18956 18810 20517 15760 16279 14049 9645 11878 14257 13779 3799 10271 17674 20429 10265 9226 15710 7740 16143 11671 11158 16237 7266 9278 11707 12579 7657 9688 21655 20275 4424 18049 15772 9286 16972 7658 7516 13897 15210 13320 16662 15120 14176 18684 9090 12407 6260 14101 34455 21206 13651 10358 9231 15093 8545 25478 22472 19919 11287 11779 7312 13007 14116 13459 6301 5521 14011 10851 13660 5822 30187 0.53_215.03 15807 25121 4992 15302 6092 15350 13666 14278 18990 8059 15124 14183 11469 21891 11969 14612 13367 7171 3760 6213 11573 13731 6760 11777 13602 7524 9319 8714 11910 8316 6629 8168 7693 11616 5898 8119 10807 8527 3952 7490 8908 6898 10036 11443 8639 10101 9079 7571 11185 8843 9343 11020 10948 4521 7904 5237 7024 18671 13820 7696 5666 3770 13617 9182 12423 6881 8956 5566 5567 5683 5821 7028 10805 4782 6611 8461 9437 8039 13105 10373 0.53_179.06 8000 11567 4705 9835 4596 7356 7351 6267 9308 6621 6451 7651 5299 9216 5993 6739 5965 4949 4318 4343 6497 5327 4234 5523 6034 4953 5323 3512 5539 4167 3937 4965 3566 4087 4189 4342 5141 4401 3792 3852 4152 4116 3922 5420 4163 5478 4366 5518 5688 3904 4837 4294 4935 4314 4843 6155 4088 6757 5315 4291 3949 2988 5980 4335 5384 3672 3329 4450 4755 4804 5275 5303 6480 3437 5135 5871 4786 4901 7301 4433 0.57_223.02 9346 10841 4769 7321 4334 8033 7680 7113 9839 6970 7457 7482 4249 8808 6601 6843 5866 5062 3616 4346 6624 5012 3450 3939 6364 4895 5425 2668 4551 3801 2607 4598 3113 4944 3109 3377 5915 4498 4205 3394 4320 4797 4195 3964 3078 4428 3588 3314 3934 3783 3590 4038 4127 3459 5313 2892 4493 4458 3452 3954 4083 4194 3282 4150 3423 4270 3992 3552 4168 3813 4272 5073 3409 6163 4451 4469 4504 5172 4028 1.36_100.93 12134 10842 11388 8763 13821 10552 10267 8248 9435 12714 3127 12748 9015 12516 17790 16206 13821 10357 11133 13697 12263 10127 11655 17805 145328 17503 13021 144594 10402 11996 10986 12303 9894 12739 10695 9670 10658 9617 31811 8914 9284 10405 8428 9323 9298 11078 13733 37703 14487 9159 12820 14309 13090 10034 11593 10983 25121 11834 8933 12497 10512 9251 11247 10877 11418 11845 14682 12098 10951 11823 11396 10573 10168 37958 13231 18193 12087 9967 12981 8777 2.10_329.25 2390 4576 3232 2708 2108 2193 2390 2528 3253 2551 2082 2539 3373 2556 3486 3535 2220 2176 2102 2578 2740 2213 5722 3139 2526 2318 2937 3256 2981 4497 3365 2339 2961 2581 3242 3560 3546 9117 7788 5045 6425 5118 6402 6560 6288 2520 6637 5899 2651 4670 5156 2629 2673 7200 3493 2847 5184 2425 4236 3400 3837 2922 2.63_673.27 4122 5413 5911 6194 3673 5935 5892 3787 5784 4734 4582 3748 4493 4671 3862 3663 4747 4817 4716 4781 4481 4957 4186 3288 3111 3727 4340 3863 4972 4715 3470 4787 3823 3977 4696 4966 4389 4414 3354 3662 4600 5112 3658 3469 4652 4562 3006 2718 4421 5961 4757 5070 4860 16627 25979 41973 22818 8675 6432 15245 6338 6730 6082 8755 4678 6180 7012 4633 4695 26242 4950 4638 6782 3431 5445 4022 5449 6159 6335 4686 2.63_671.27 9226 12252 15284 14751 9873 14788 16288 9854 15590 11830 12924 9447 11599 11555 9684 9444 12064 12381 12202 11326 10699 11942 11327 8723 8249 9994 10092 9041 13221 12235 9277 11931 9802 10646 12080 12345 11900 12029 8479 10715 11883 12800 9396 7442 11830 11102 7331 6774 10307 14699 11126 12658 11377 43475 73554 113357 62146 20868 15539 41198 16118 16490 14608 23045 11689 15951 17111 11917 12564 70769 13772 12484 17912 9424 13963 11470 13561 15615 16041 12727 2.65_917.55 3974 3306 2216 2483 2161 9949 2143 8601 3128 3023 4451 9226 2230 7666 5570 4017 3567 5724 2603 2401 4833 3075 6916 2990 6190 3636 2663 4329 2842 2051 3645 5965 4207 3871 3827 10973 5173 3942 3430 5722 31279 55092 104048 36540 16572 22939 42330 6126 9725 58648 6654 6333 9399 31442 4028 5822 61126 2964 3592 10912 17412 3157 6585 3815 52097 4569 2.66_381.23 2890 2176 2067 2091 3062 3171 2602 2446 3355 3599 2154 3527 2793 2631 2750 3009 2067 2505 3101 2498 3504 2394 3034 2964 2089 2714 2397 2016 2669 3203 3276 2870 3239 4335 2768 2504 2667 3175 4650 3883 4398 4273 3805 3847 4572 2904 3109 4940 2684 3343 3640 4430 2649 2970 4368 2356 2392 3546 4159 2502 3436 2269 4644 2727 2.66_363.25 3505 2725 2203 4830 2052 4815 2449 2414 3611 5488 2170 4780 3657 3379 2912 3746 2428 3717 2757 3936 2695 3746 2930 2395 3473 2356 2305 2959 3650 3492 2818 3740 7123 2968 3120 2719 3570 8138 9440 13346 6958 6724 9378 10263 4172 5090 20752 3017 4077 4607 11542 3128 4092 10402 2346 3078 5285 7536 2908 3845 2490 18472 3533 2.66_379.23 3901 3286 2038 2519 2292 2014 4457 2983 4432 3188 3078 4367 4996 2564 4492 3971 3704 3522 4066 2840 3297 4281 3077 4420 3400 2339 4350 3583 2998 2246 4064 3149 3065 3197 3150 4274 4325 2261 3510 4102 2232 5155 3309 3722 3066 4712 5762 6579 7270 5576 5787 5610 6451 4247 4694 7292 4026 4314 4611 5984 3594 4113 6444 2877 3480 4482 5040 3721 4233 3113 7268 3305 2.83_465.30 2971 5471 3994 3698 3588 4222 4161 4492 4177 2654 3071 4317 4840 2253 3541 3962 2797 5266 3760 2955 3052 4931 3123 2439 2317 4350 2642 3420 3684 2737 2096 4855 3385 3482 4451 4665 2900 4371 2628 2703 3234 6693 3162 2878 3271 4087 2753 3675 3697 3329 5452 4242 4779 3945 5817 4408 4324 3474 3529 3014 4253 4939 2687 6656 4693 3597 4590 2900 5037 7687 6342 4128 4280 4380 3762 4254 4254 5975 5500 2715 3.46_311.30 34756 35308 31970 24001 22780 16878 29342 25362 33340 39498 24600 22346 21364 18365 19636 15362 26481 23430 22817 27302 29285 36555 21121 15140 22716 18401 22084 19477 28922 32551 21448 32619 21621 17849 26475 19067 24016 23325 14418 24094 30376 33751 17467 17036 18621 15634 16663 20220 28702 41620 4434 3813 4258 14123 17669 19720 17138 9720 8495 8635 26130 10353 20226 24272 10895 12534 13512 16387 16471 10466 6903 5228 9156 5093 17614 10101 13144 10203 10069 6959 4.53_885.55 7656 13050 10250 9951 9902 9638 11311 8900 11699 7151 10389 7360 6379 7450 8962 6655 7745 8156 7350 5607 12033 7637 6352 7371 9670 7321 6271 10174 6681 5105 11949 8781 8342 9161 10307 7796 7189 4712 7063 8436 12101 7761 7101 8055 9708 7703 6546 9129 9290 10418 9196 11100 3209 2365 3561 7136 8154 2275 6842 6963 7218 10732 9401 6408 9923 7673 6258 7033 5993 4192 7249 8533 8911 7688 4235 9341 10319 7107 4.58_784.55 8512 9013 9272 9285 9848 5092 9255 6111 7107 7445 4631 6236 4543 7400 7094 6475 8037 8315 5302 6536 8427 7742 7719 7449 9117 6370 8597 5223 7733 8236 4454 10478 9175 6238 6969 7343 6501 9263 5670 6160 7440 12290 8140 13689 6975 10962 4935 4935 5782 5153 4.70_772.55 3108 7551 7746 6009 8436 4492 7856 4700 5447 4964 5754 5569 3602 5530 3387 5496 3124 4182 4179 2877 6475 5871 4114 5267 3877 5504 2563 4788 4413 2009 6656 5735 4631 7257 3493 5650 2976 2412 4860 3359 7677 6239 4766 6711 3421 5917 3204 4392 5693 19919 10659 15830 5974 4893 4554 9091 5394 5394 3809 11997 9595 8473 17482 13200 5477 6101 7308 9146 5259 19647 5566 21159 16795 14971 10294 3718 14251 9038 9552 4.75_887.56 2499 3504 3362 3576 3888 3556 3582 5161 3925 3472 2851 2983 2655 3244 3483 2969 4963 3245 3891 2491 3640 3499 3110 3280 3788 2995 4403 3768 3788 3831 3803 3677 4423 4402 3176 5021 2244 3687 3147 3644 3671 3587 3958 4117 3274 4838 4018 4757 8050 5913 6569 2222 6502 6435 2226 6342 6771 3700 5374 5761 4887 5594 4703 3176 3122 7031 2914 4387 7010 6290 4539 5050 5397 5618 8062 4.87_810.56 4641 3813 2889 4835 3530 3999 2138 3077 3540 2717 3227 2009 3125 3304 2732 2647 3167 3267 3593 2071 3548 3577 2579 3039 3349 3430 3592 2498 4628 3551 3097 3383 2161 6361 3675 2637 4355 2828 3684 2833 3855 6877 2909 6432 2524 2377 2328 2156 4222 3495 4086 6280 3750 2679 3792 3691 3024 3270 3974 3408 5032 3794 4807 2915 5116 2986 3252 4.91_449.31 3626 4023 4164 3850 4289 3996 3922 4253 3759 4035 2801 4016 3710 4061 3965 4149 3639 3994 3931 3689 3906 4178 4216 3837 3926 4387 4556 4019 4052 4065 3584 4079 3996 4248 4177 4040 4126 3748 3669 3582 3818 4040 3888 4026 3767 3992 3940 4374 3790 4122 4075 4264 4095 3790 4131 3662 4332 4285 3862 4052 4096 4007 3765 4384 4183 4091 3880 3723 4163 3969 4291 3823 3724 4349 4395 4229 3697 4066 4384 3696 5.01_786.56 4730 7068 9324 7833 11308 6249 10369 5417 8968 8226 5731 8372 5039 8912 4416 7258 3524 6552 5511 3860 6093 8447 5159 8423 4321 7642 2576 7543 7603 2913 6737 8839 5510 9474 4596 8027 3860 4677 7744 4960 9657 8886 6305 9010 4384 8326 3787 7080 9824 32306 18620 26331 9059 6303 6966 11280 7944 7593 6491 19392 16470 12015 19553 22623 9121 9193 12313 12264 6778 29013 12005 31966 26969 20726 13101 7720 24896 12629 22706 5.15_802.56 3851 3384 4026 2189 3409 3503 4132 4724 2333 3128 3243 2395 2795 2361 3380 3850 3173 3734 2809 2392 2631 2464 2735 4066 2437 3660 3144 3293 2488 3031 2516 3220 2524 2410 2979 4524 3125 2802 2246 3273 2600 2864 4451 3477 8134 7669 8040 2753 2390 3171 5070 4317 2664 2677 4782 2041 5387 5846 2021 2960 2247 4480 2094 6985 3292 5576 7463 4791 6042 2759 5288 6679 6527 5.20_774.56 3588 5559 5641 5012 8178 5457 6832 4693 5294 4509 4911 5754 3782 7879 6060 7785 4488 5122 5053 4653 5671 5893 4123 7427 6504 7171 4291 5277 5471 3645 5636 5664 4878 9948 6944 7388 4603 3418 5498 4174 8400 6092 4333 9184 6468 6515 4472 4852 6326 5357 2815 2944 9788 9638 6027 10628 3313 6418 8313 3531 4733 6040 3303 3210 4520 3566 6798 5330 5897 3879 6924 4875 6437 4182 4865 7060 3733 2649 5.42_762.56 3829 5110 3705 4849 3065 4415 3045 3325 3737 4164 5794 3213 5536 3417 4426 2853 3320 5610 2734 5190 4887 3337 4742 3574 4664 3117 3339 6152 2381 4901 5533 3616 6972 3474 4772 2644 2109 4962 2562 6657 5969 3691 5845 3243 4331 2633 3763 5947 6390 3899 5193 6823 6508 5716 9571 2362 2818 4508 8061 3982 4334 6859 3601 4711 3875 3446 5056 4733 6553 3679 5943 6016 5857 3316 3882 6521 3966 4112 5.49_788.58 8184 9659 11848 9784 18980 13558 17179 10575 14453 11053 7564 7957 11965 7507 17688 14789 16855 8866 13129 12307 8722 10203 13041 8009 17179 15001 17420 8542 13880 13589 6340 9758 12926 8556 23017 16069 15860 9487 7487 12387 8695 23129 13609 8175 16953 13893 14639 7828 12644 15542 25894 10263 11754 23156 20015 14205 27201 5208 8321 16496 21834 11714 8628 14311 10385 6938 9668 6480 15858 9161 21307 12540 24490 19897 22809 12369 14842 27664 13693 11371 5.50_790.61 3485 4960 4665 4041 4088 5008 4620 4319 4624 3466 4185 4068 2788 4781 4865 4352 4244 4404 3646 4015 4699 3907 3015 4072 4779 4767 3536 4393 3689 3224 4724 3978 3658 5689 5160 4731 4021 2496 3355 3572 6566 4350 3256 4751 4813 4159 3888 4461 4268 6947 4913 7416 5834 5046 4448 7328 4255 4013 4856 6273 4628 4377 7005 4700 4432 5541 3797 4897 5050 6114 5180 7168 6851 6353 5498 5587 7961 6261 4489 5.65_820.58 5255 5429 5983 5284 5393 7802 5978 7652 7389 4491 2116 5045 5057 4770 5489 7444 5478 7408 6384 5661 5878 5644 5707 4450 5181 7680 5367 6593 6994 5184 4675 5370 5315 5033 6078 9271 5270 7035 5025 4921 5773 7460 4852 4785 5601 8023 5274 6512 6934 5630 6711 4800 4643 5872 5661 5817 7055 5261 6743 6332 5226 5138 4665 5458 4244 4626 6749 3459 6691 5941 5034 4161 5760 4818 5559 5783 7314 4446 4090 4364 5.79_926.61 2225 3530 3086 2914 2774 2786 3150 3234 3335 2143 2769 2743 2322 2329 2618 2381 2768 2711 2470 2158 3361 2448 2575 2074 2464 2676 2459 3149 2285 2941 2923 2765 2543 2902 2520 2546 2269 2504 2549 3195 2839 2741 2445 2432 2759 2633 2707 2633 4604 4441 4808 2190 2012 2576 3604 3336 3021 3343 2115 3804 4260 2564 2364 2045 3316 4326 2467 4234 4373 3576 4190 2365 4267 3743 4177 5.93_872.64 16542 13481 10558 13181 12042 6087 18571 12963 12785 11089 16522 12988 11549 10564 12112 6750 19304 15152 12748 10750 17982 14272 11944 11131 12375 7351 21026 12817 13838 10769 15040 13835 12308 11930 14467 7185 18714 15297 9185 10381 18374 15988 13226 11906 12677 7326 16984 14271 10897 11676 8881 5668 6879 9092 8879 4425 9691 6322 6529 3805 14636 10312 10784 15110 11150 7995 9314 10922 12109 6928 8507 5758 8493 11539 9705 14563 6710 9436 6848 8859 5.97_856.61 2827 2127 2244 2166 2241 2466 2456 2205 2039 2312 2414 2351 2435 2630 2612 2370 2592 2341 2260 2218 2386 2575 2562 2341 2169 2093 2156 2442 2531 2057 3294 3485 2787 3196 4284 3903 2801 3334 2488 2914 2314 2939 4018 2942 2392 3609 2672 2058 2704 2638 2379 2743 3067 2114 3337 6.16_816.61 2135 2176 2406 2721 2549 2755 2434 2772 2067 2473 2734 2917 2339 2814 2233 2223 2648 2423 2951 3097 2286 3043 2685 2901 2200 2526 3400 2577 2530 2472 2761 2080 2654 2144 4441 2497 3536 3088 3130 3162 2143 2896 3342 2657 2922 2690 2311 2316 3617 3853 3436 3850 4548 3394 2364 4486 3711 3100 6.30_822.58_6.30_846.62 72045 84185 73793 83237 86962 92309 89000 90068 93064 62884 69831 73282 67819 60947 79354 105130 74135 91771 83235 78024 73948 89916 72952 67583 81301 110453 79210 82359 87828 73612 58659 81393 73334 75887 95212 115383 74902 92524 52607 67404 75553 109427 70150 69064 82243 106386 70207 80345 80146 80480 86677 68144 60594 69361 71408 54813 93475 63183 78480 61897 69786 59385 48092 63470 55480 55509 74993 41690 76428 67084 73209 50369 79677 73280 74559 74870 70895 92274 83932 54771 6.30_928.63 4759 5446 5127 5121 5401 5740 5641 5386 5689 4252 4582 5231 4389 4133 5270 6079 5027 5734 5240 4798 5213 5501 5081 4722 4799 6587 4952 4968 5311 4796 3774 5372 5009 4988 5749 6814 5418 5644 3378 4501 4942 6385 4861 4240 5046 6105 4523 4994 4930 5406 5709 4467 4305 4651 4870 3955 5639 4693 5224 4351 4545 3986 3385 4256 3754 3682 5217 2859 5115 4569 4667 3513 5226 4952 4791 4947 4994 6052 5297 3685 6.31_832.61 12110 14031 13303 14476 14758 14945 14982 15014 15438 10304 11449 12367 11911 10553 13482 17271 12134 15699 13552 12471 12762 14661 11782 11135 13489 18984 13327 13809 15240 11860 10178 13771 12242 12507 16233 19660 12642 15609 8911 11266 12369 18668 12056 11992 13038 17287 11341 13425 13901 13507 15103 11586 9814 11404 12138 9579 16391 11303 13065 10557 11655 9818 8424 10660 9450 9726 12783 7028 13213 11369 11941 8957 12791 13066 13508 12586 11733 15719 14323 9013 6.31_491.36 6806 7046 6678 6463 6707 6489 7171 6210 6178 6752 6744 7129 6441 7016 7053 6791 6976 6311 6533 6337 7056 6304 7167 7137 7152 6669 7433 7079 6943 6911 7060 7187 6412 7147 6451 6180 7113 6627 6643 6505 6048 6354 6125 6927 6109 6548 6585 6988 7033 6503 6811 6994 6572 6906 7029 6942 7281 6973 6345 7005 6554 5950 6313 6807 6549 6981 7122 5823 6839 7166 7273 6947 6773 6980 6755 6754 6957 6662 7229 6252 6.31_506.38 4246 4212 4561 4193 4819 4093 4206 4459 4468 4079 2458 4136 3681 4057 4219 4762 4166 4323 3955 3793 4332 4338 4107 4396 4760 4508 4567 4452 5039 3766 3852 4563 3856 4147 4494 4234 3998 4463 4253 3769 3948 4474 3761 4264 4018 4232 4236 4214 4385 4089 3997 4239 3977 4370 4351 3862 4668 3802 4361 4344 4134 3599 3626 4023 4004 4099 4176 3583 4295 4377 4118 3696 4187 4297 4439 4006 3900 4489 4089 3667 6.31_463.33 4768 5000 4704 4899 5165 5030 5271 4997 5077 4552 4847 4922 4717 4539 5531 4972 4763 5099 5154 4735 6042 5118 4811 4465 4935 5655 5166 4862 5145 4805 4769 5042 4595 4983 5300 5266 5081 5117 4276 4333 4884 5477 4520 4671 4594 5155 4903 5442 4895 4921 5133 5154 4616 4697 5195 4508 5508 4676 4993 4783 4879 4249 4417 4683 4080 4630 5057 3598 4966 5338 4869 3692 4981 4977 4956 4999 4971 4677 5115 4112 6.45_874.65 20712 14584 10398 12841 14411 7928 21473 17455 14428 11578 24361 15655 12492 11722 16713 12231 26597 23460 17987 13431 25899 16781 14004 12744 17170 12761 27787 22136 17737 14671 20602 16537 14317 14479 20396 14483 27273 25522 9909 13378 24196 25572 14504 13680 16736 12865 22981 22441 14389 13642 5507 3090 2750 12634 12140 5104 13350 2434 3612 4478 12664 4993 6030 8550 4095 4697 6006 5865 10173 5484 4500 4034 4905 5589 6306 9300 6420 6722 4325 4103 7.03_876.67 7573 9595 5942 7664 9762 5820 10016 9283 8558 6859 9706 9724 6590 5806 10196 8031 10629 14246 9019 7962 12196 13661 7821 6803 12064 8385 11251 12774 10731 9085 8249 13120 8476 7746 14497 10572 10761 14477 4922 8270 11578 14390 9205 6871 10124 7539 9766 11258 8138 9750 7108 6515 10296 7501 4709 5172 8.44_491.36 2379 2441 2347 2496 2278 2244 2402 2372 2129 2087 2021 2166 2288 2231 2327 2034 3376 2431 2136 2292 2377 2153 2831 2475 2155 2100 2336 2518 2505 2179 2330 2072 3189 5551 4660 6432 6114 6262 5919 5492 6310 3860 2609 4321 5820 5420 5182 5025 2484 3876 6070 2435 4589 3693 2951 3038 2743 2669 4490 8.45_819.64 2179 2158 2281 2380 2125 2135 2668 2419 2362 2255 2287 3129 2412 2064 2181 2136 2068 2227 2297 2072 2011 2264 2079 2171 2561 4019 3585 4510 5465 6032 5333 4387 4720 3474 2234 3333 4553 4349 3759 3679 2140 3453 5138 2015 3601 2991 2430 2607 2431 2276 3652 9.88_805.62 4172 5582 4182 4572 5849 5426 5317 5112 4723 4594 4142 5688 4168 3842 5901 5442 4890 5558 4997 4443 4521 5765 5182 4127 5400 6047 5290 4598 5426 4325 4091 6485 4724 5055 5847 5577 5148 5096 3823 3808 5769 6072 4648 4703 5703 5824 4713 4736 5419 4973 5257 2968 3928 4957 3316 2324 5165 4014 2488 2985 3867 2077 3660 6092 2458 3751 4791 3602 5300 5157 5144 4581 4900 2922 3424 5517 3515 5319 2916 2454 9.88_759.62 2645 4009 2829 3187 3778 3823 3484 3330 2996 2830 2327 3624 2784 2508 4216 4166 3155 3564 3057 2700 3073 4073 3075 2682 3470 4292 3559 3135 4008 2953 2507 3994 3125 3130 4099 3853 3057 3432 2462 2680 3730 4386 3110 2986 3977 3771 2820 3302 3135 3126 2385 3020 2047 3192 2414 2084 2243 2921 2263 4047 2417 2750 3184 2190 3461 3285 3149 3566 2269 2504 3507 2485 4112 2091 10.36_820.64 3895 4437 4281 3782 4169 4185 3954 3747 4067 3784 3593 3942 3923 3746 3893 4465 3764 3898 3946 3952 3660 3904 4570 3804 3805 4285 3996 3882 3828 3713 3621 4022 3871 3857 4025 3986 3786 3897 3799 3697 3923 4131 3909 3881 3497 4172 3795 3999 3665 3952 3961 3701 3885 3927 3674 3922 3433 3662 3721 3810 4141 4125 3747 4246 3996 4066 4135 4281 3808 4433 4781 3925 3860 3518 3805 4144 3541 4125 4079 3582 10.43_1001.81 3458 3500 3588 3562 3573 3580 3524 3061 3156 3766 4558 3843 3480 3642 3555 3649 3671 3480 3393 3453 3538 3384 3605 3508 3332 3513 3554 3521 3403 3465 3453 3596 3539 3670 3492 3246 3431 3541 3857 3385 3304 3257 3230 3622 3248 3359 3468 3508 4845 3381 3556 3364 3479 3371 3565 3463 3348 3529 4540 3653 3398 3436 3429 3457 3742 3568 3312 3631 3399 3519 3377 3570 3277 3294 3468 3438 3426 3295 3367 3302 10.57_230.05 2385 2809 2760 2361 2922 2550 2894 2640 2558 2896 2318 2626 2347 2261 2415 2528 2613 2557 2353 2081 2404 2544 2252 2406 2420 2446 2337 2688 2646 2138 2258 2704 2403 2627 2465 2586 2261 2330 2379 2041 2184 2970 2375 2424 2054 2230 2494 2558 2472 2461 4226 4083 3987 2453 2268 2340 3195 3732 2324 2349 2638 2944 3263 4115 4260 2899 3324 2962 2639 2801 2130 3209 3461 4204 3326 3585 2480 3471 3477 2937 10.58_507.38 3462 3447 3765 3436 3598 3301 3549 3254 3182 3604 3377 3473 3439 3694 3694 3604 3523 3455 3546 3286 3617 3347 3579 3705 3624 3617 3509 3598 3500 3689 3535 3704 3548 3366 3593 3678 3720 3750 3620 3500 3436 3325 3420 3536 3347 3484 3641 3795 3824 3389 3482 3673 3631 3869 3601 3663 3612 3408 3537 3591 3360 3218 3528 3472 3716 3816 3527 3338 3888 3691 3304 3443 3270 3437 3345 3595 3640 3009 3445 3317 10.58_464.33 5398 4944 4793 4694 5294 5005 4947 4706 4502 5250 4295 5110 4447 4339 4796 4833 4665 4252 4539 4303 4562 4640 4595 4758 4737 6882 4704 5239 4538 4280 4706 5130 4360 4873 4721 4658 4504 4902 4915 4205 4386 4742 4583 4894 4407 4776 4686 5231 4433 4712 4665 5300 5059 4700 4883 4481 5026 4682 4858 4935 4519 4528 5275 4592 4878 4720 4596 4723 5164 4603 5078 4827 4536 4908 4904 5129 4611 5208 5002 4378 10.72_449.31 4154 4225 3974 3650 3889 4060 4052 4234 3830 4140 3795 4148 3562 4167 4189 4211 4292 4015 3974 3899 4046 3706 4291 3979 4109 4505 4016 4056 4103 4041 4074 4006 3690 4127 4270 4020 3946 4080 4005 3813 3807 3817 3493 4162 3799 3985 4054 4138 4271 3560 3894 3936 4013 4006 4103 4291 4217 3648 3602 3973 3494 3680 3737 4089 4018 4019 4087 4217 4493 3922 3905 4119 3586 4053 4211 4242 3806 3720 4064 3436 10.75_419.32 3607 3922 4035 3645 4177 3828 4908 3881 4893 3863 3778 3827 3563 3836 3988 4245 4028 3676 3727 3481 3813 3610 3455 3751 3719 4404 3726 3793 3642 3677 3731 4016 3340 3587 4064 3829 3726 3749 3995 3736 3799 3899 3502 3899 3621 3839 3754 3742 3856 3473 4067 3773 3563 3758 3542 3752 4209 3765 4021 3894 3699 3251 3733 3850 3822 3811 3518 3667 3627 3949 4037 3687 3589 3776 3531 3986 3702 3468 3550 3205 11.01_507.38 3511 3591 3805 3524 3450 3851 3622 3506 3567 3725 3505 4009 3376 3276 3575 4048 3599 3710 3684 3178 3584 3381 3827 3613 3618 3549 3542 3656 3472 3336 3366 3840 3599 3779 3612 3603 3522 3944 3119 3021 3530 3679 3191 3661 3493 3458 3552 3930 2913 3534 3770 3425 3526 3878 3534 3569 3612 3649 3537 3725 3344 3209 3492 3737 3506 3410 3824 3328 3585 3424 3300 3548 3409 3526 3552 3778 3318 3523 3674 3452 11.06_379.27 3720 3380 3671 3421 3365 3372 3654 3352 3487 3660 3564 3695 3436 3526 3666 3719 3373 3588 3581 3643 3842 3741 3558 3566 3516 3856 3617 3390 3662 3437 3597 3972 3628 3453 3679 3508 3631 3489 3757 3369 3482 3626 3675 3910 3344 3343 3650 3470 3172 3656 3547 3354 3844 3794 3470 3826 3720 3898 3171 3387 3352 3519 3831 3755 3611 4143 4024 3747 3586 3537 3422 3578 3921 4366 3669 3888 3683 3487 3674 3441 11.07_831.66 3486 3633 3623 3417 3665 3790 3528 3904 3556 4179 3319 3628 3616 3854 3755 3800 3809 3684 3560 3466 3668 3288 3620 3643 3675 3416 3586 3630 3607 3573 4033 3495 3460 3624 3550 3546 3838 3659 3549 3406 3816 3429 3607 3458 3222 3505 3798 3429 3477 3504 3615 3364 3691 3743 3480 3747 3853 3183 3721 3303 3474 3717 3661 3476 3885 3728 3658 3675 3614 3570 3537 3798 3485 3588 3740 3636 3402 3476 3071 11.15_1002.81 4078 5056 3525 3976 4371 4855 6110 4683 3306 4235 4137 4184 3808 3814 4184 4019 4326 6633 4795 4056 4644 4262 3640 4237 4188 5342 4311 3852 4972 4182 3736 4388 3894 4777 4326 5232 4345 4838 4175 3947 4127 5473 4147 3932 4135 4855 4102 4128 5108 4352 5286 4438 4249 4538 4560 4135 4350 4077 3990 4044 4330 3518 3996 5337 3916 4389 4156 4165 4931 4390 3592 4039 3697 4122 4281 4506 3783 4339 4641 4108 11.18_941.79 3624 3586 3715 3369 3513 3450 3973 3921 3664 4005 3466 3534 3631 3331 3969 3883 3387 4189 3684 3635 3492 3391 3447 3745 3339 3876 3586 3414 4095 3518 3206 3628 3387 4023 4511 3844 3585 3800 3620 3497 3292 3838 3734 3530 3405 3527 3391 3496 3893 3591 4190 3553 3929 3887 3615 3687 3666 3703 3586 3536 3605 3308 3098 4235 3521 3699 3500 3509 3661 3625 4106 3647 3644 3474 3717 3427 3504 3470 3701 3310 11.42_1001.81 5211 9449 8701 6397 6310 9685 9030 8389 7157 6111 5317 5839 5675 6443 9507 8923 5605 8077 8925 9103 6420 6405 5897 5405 6007 9206 9372 6468 9117 9071 5453 8886 9170 9073 9133 6030 9579 9129 5393 5094 6269 8515 5164 6365 6371 6216 5862 6176 9474 6016 6741 5749 6381 5934 5464 5281 6063 5607 5598 5361 5481 5007 4987 6936 5848 5633 5973 9157 5313 5454 4503 5927 5336 5270 6236 5221 5549 5882 5809 5124 12.74_158.98 23174 13507 13023 15162 15759 11693 20046 12541 12243 13502 39085 24867 10227 18067 22831 16554 13975 12153 12171 12762 17068 11813 10085 15921 23686 46801 17357 18658 59122 20655 15668 13159 11032 17654 14564 11610 20507 15592 22273 11022 11828 11498 11063 15367 34804 12431 18644 23532 162660 12938 14438 17128 14016 13329 15219 13257 172340 31265 12405 21687 11182 6759 15939 17891 17869 14880 15659 16284 12292 14578 6867 6067 18911 17616 14727 14044 15256 18104 14229 14545 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Identifier m/z RT Adduct Ceramide (d34:1) 5.72_572.48 572.481 5.716 [M+Cl]- FA (16:1) (palmitoleic acid) 1.83_253.22 253.2174 1.826 [M-H]- FA (17:1) (heptadecenoic acid) 2.20_267.23 267.233 2.203 [M-H]- FA (18:1) (oleic acid) 2.66_281.25 281.2488 2.663 [M-H]- FA (18:2) (linoleic acid) 2.04_279.23 279.233 2.037 [M-H]- FA (18:3) (linolenic acid) 1.59_277.22 277.2172 1.59 [M-H]- FA (20:4) (arachidonic acid) 1.91_303.23 303.2329 1.909 [M-H]- CSH_negESI #387 5.03_841.58 841.5789 5.031 CSH_negESI #590 4.72_814.56 814.5596 4.716 CSH_negESI #599 4.83_737.56 737.538 4.828 CSH_negESI #644 5.33_842.59 842.5907 5.327 CSH_negESI #743 7.22_859.69 859.6902 7.22 0.50_187.01 0.50_187.01 187.0076 0.5 0.53_215.03 0.53_215.03 215.0338 0.526 0.53_179.06 0.53_179.06 179.0558 0.532 0.57_223.02 0.57_223.02 223.0204 0.57 1.36_100.93 1.36_100.93 100.9343 1.356 2.10_329.25 2.10_329.25 329.2486 2.103 2.63_673.27 2.63_673.27 673.2659 2.632 2.63_671.27 2.63_671.27 671.2669 2.633 2.65_917.55 2.65_917.55 917.5461 2.65 2.66_381.23 2.66_381.23 381.2283 2.662 2.66_363.25 2.66_363.25 363.2516 2.663 2.66_379.23 2.66_379.23 379.2333 2.663 2.83_465.30 2.83_465.30 465.3045 2.827 3.46_311.30 3.46_311.30 311.2955 3.465 4.53_885.55 4.53_885.55 885.5504 4.534 4.58_784.55 4.58_784.55 784.549 4.577 4.70_772.55 4.70_772.55 772.5487 4.696 4.75_887.56 4.75_887.56 887.5645 4.754 4.87_810.56 4.87_810.56 810.5645 4.868 4.91_449.31 4.91_449.31 449.3119 4.906 5.01_786.56 5.01_786.56 786.5644 5.013 5.15_802.56 5.15_802.56 802.5599 5.151 5.20_774.56 5.20_774.56 774.5645 5.197 5.42_762.56 5.42_762.56 762.5649 5.42 5.49_788.58 5.49_788.58 788.5803 5.494 5.50_790.61 5.50_790.61 790.606 5.502 5.65_820.58 5.65_820.58 820.578 5.649 5.79_926.61 5.79_926.61 926.6097 5.79 5.93_872.64 5.93_872.64 872.6362 5.929 5.97_856.61 5.97_856.61 856.6065 5.967 6.16_816.61 6.16_816.61 816.6102 6.159 6.30_822.58_6.30_846.62 6.30_822.58_6.30_846.62 822.5790_846.6227 6.3015 6.30_928.63 6.30_928.63 928.6251 6.302 6.31_832.61 6.31_832.61 832.6068 6.306 6.31_491.36 6.31_491.36 491.3589 6.31 6.31_506.38 6.31_506.38 506.377 6.311 6.31_463.33 6.31_463.33 463.3276 6.312 6.45_874.65 6.45_874.65 874.6533 6.45 7.03_876.67 7.03_876.67 876.6694 7.028 8.44_491.36 8.44_491.36 491.3589 8.44 8.45_819.64 8.45_819.64 819.6378 8.447 9.88_805.62 9.88_805.62 805.6228 9.878 9.88_759.62 9.88_759.62 759.6177 9.882 10.36_820.64 10.36_820.64 820.6403 10.364 10.43_1001.81 10.43_1001.81 1001.8056 10.432 10.57_230.05 10.57_230.05 230.0491 10.574 10.58_507.38 10.58_507.38 507.376 10.58 10.58_464.33 10.58_464.33 464.3302 10.583 10.72_449.31 10.72_449.31 449.3122 10.721 10.75_419.32 10.75_419.32 419.3193 10.747 11.01_507.38 11.01_507.38 507.376 11.009 11.06_379.27 11.06_379.27 379.2724 11.058 11.07_831.66 11.07_831.66 831.6572 11.073 11.15_1002.81 11.15_1002.81 1002.8085 11.148 11.18_941.79 11.18_941.79 941.785 11.176 11.42_1001.81 11.42_1001.81 1001.8057 11.423 12.74_158.98 12.74_158.98 158.9755 12.744 METABOLITES_END #END