#METABOLOMICS WORKBENCH amitch_20160114_9407421_mwtab.txt DATATRACK_ID:481 kenanazam_20160121_111839 STUDY_ID:ST000337 ANALYSIS_ID:AN000545 PROJECT_ID:PR000269 VERSION 1 CREATED_ON January 21, 2016, 11:48 am #PROJECT PR:PROJECT_TITLE Metabolomics approach to allograft assessment in liver transplantation PR:PROJECT_TYPE Retrospective analysis of biobank samples PR:PROJECT_SUMMARY Organ shortage is the primary limiting factor in liver transplantation and has PR:PROJECT_SUMMARY led to expanded utilization of livers from marginal donors. Assessment of liver PR:PROJECT_SUMMARY allograft quality is currently based on clinical factors such as donor age, PR:PROJECT_SUMMARY cause of death, liver function, routine clinical laboratory values and extent of PR:PROJECT_SUMMARY graft steatosis (fat content). Expanded utilization necessitates a more detailed PR:PROJECT_SUMMARY assessment of liver allograft quality to guide decisions regarding suitability PR:PROJECT_SUMMARY for transplantation. Using a metabolomics approach, we plan to capture molecular PR:PROJECT_SUMMARY snapshots of the physiologic state of the graft and to elucidate biomarkers of PR:PROJECT_SUMMARY allograft quality and graft function post-transplant. PR:INSTITUTE Ochsner Multi-Organ Transplant Institute / SECIM PR:DEPARTMENT Abdominal Organ Transplantation PR:LABORATORY - PR:LAST_NAME Seal PR:FIRST_NAME John PR:ADDRESS 1514 Jefferson Highway, New Orleans, LA 70121 PR:EMAIL John.Seal@ochsner.org PR:PHONE 504-232-4253 PR:FUNDING_SOURCE SECIM Pilot and Feasibility Award #STUDY ST:STUDY_TITLE Metabolomics Approach to Allograft Assessment in Liver Transplantation ST:STUDY_TYPE Retrospective analysis of biobanked liver tissue from organ donors ST:STUDY_SUMMARY This pilot study is designed to apply several types of metabolomic analysis for ST:STUDY_SUMMARY liver allograft assessment with the aim of identifying candidate biomarkers for ST:STUDY_SUMMARY allograft function and to develop a methodology that could be applied to larger ST:STUDY_SUMMARY scale studies. The three main categories of metabolomic analysis are reflected ST:STUDY_SUMMARY in each of the specific aims, including a targeted profiling of central carbon ST:STUDY_SUMMARY metabolism, open lipidomic and metabolomic profiling for hypothesis generation ST:STUDY_SUMMARY and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers ST:STUDY_SUMMARY potential benefits that could be optimized in a protocol for larger scale ST:STUDY_SUMMARY studies depending the results of the pilot investigations. ST:INSTITUTE Ochsner Multi-Organ Transplant Institute ST:DEPARTMENT SECIM ST:LABORATORY Abdominal Organ Transplantation ST:LAST_NAME Seal ST:FIRST_NAME John ST:ADDRESS 1514 Jefferson Highway, New Orleans, LA 70121 ST:EMAIL John.Seal@ochsner.org ST:PHONE 504-232-4253 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS Targeted analysis and open profiling: Control group - standard criteria donor ST:TOTAL_SUBJECTS (N=15); Experimental group - donation-after-cardiac-death donors (N=10). ST:TOTAL_SUBJECTS MALDI-IMS - standard criteria donor (N=10) #SUBJECT SU:SUBJECT_TYPE Human tissue (Decendent) SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENOTYPE_STRAIN Not applicable SU:AGE_OR_AGE_RANGE Variable SU:WEIGHT_OR_WEIGHT_RANGE Variable SU:HEIGHT_OR_HEIGHT_RANGE Variable SU:GENDER Variable SU:HUMAN_RACE Variable SU:HUMAN_ETHNICITY Variable SU:HUMAN_TRIAL_TYPE Not applicable SU:HUMAN_LIFESTYLE_FACTORS Not applicable SU:HUMAN_MEDICATIONS Not applicable SU:HUMAN_PRESCRIPTION_OTC Not applicable SU:HUMAN_SMOKING_STATUS Not applicable SU:HUMAN_ALCOHOL_DRUG_USE Not applicable SU:HUMAN_NUTRITION Not applicable SU:HUMAN_INCLUSION_CRITERIA Deceased organ donor with consent for use of tissue for research SU:HUMAN_EXCLUSION_CRITERIA None SU:ANIMAL_ANIMAL_SUPPLIER in house/in house SU:SUBJECT_COMMENTS Liver biopsy is routinely performed on livers used for solid organ SU:SUBJECT_COMMENTS transplantation. The tissue is from decedent subjects. Consent for use of tissue SU:SUBJECT_COMMENTS is obtained at the time of organ donation by the local organ procurement SU:SUBJECT_COMMENTS organization. #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 114291 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=1/20/2015; OPO=AROR; ABO=A; AGE (years)=46; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=163; DONOR WEIGHT (kg)=74.4; Hep C Positive Donor=NO; Cold Ischemia (min)=327; Cold Ischemia (Hrs)=5.45; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 116707 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=2/7/2015; OPO=LAOP; ABO=A; AGE (years)=52; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=175.26; DONOR WEIGHT (kg)=96.79; Hep C Positive Donor=NO; Cold Ischemia (min)=278; Cold Ischemia (Hrs)=4.63; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 115165 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=4/1/2015; OPO=TXSB; ABO=O; AGE (years)=59; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=165; DONOR WEIGHT (kg)=63; Hep C Positive Donor=NO; Cold Ischemia (min)=323; Cold Ischemia (Hrs)=5.38; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 115333 DONOR CAUSE OF DEATH:Cerebrovascular/Stroke RECOVERY DATE=4/10/2015; OPO=PADV; ABO=B; AGE (years)=44; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=180.34; DONOR WEIGHT (kg)=59; Hep C Positive Donor=NO; Cold Ischemia (min)=454; Cold Ischemia (Hrs)=7.57; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 115342 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=4/10/2015; OPO=LAOP; ABO=B; AGE (years)=46; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=180.34; DONOR WEIGHT (kg)=103.78; Hep C Positive Donor=NO; Cold Ischemia (min)=216; Cold Ischemia (Hrs)=3.60; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 117751 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=6/25/2015; OPO=LAOP; ABO=O; AGE (years)=48; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=182.9; DONOR WEIGHT (kg)=105.9; Hep C Positive Donor=NO; Cold Ischemia (min)=228; Cold Ischemia (Hrs)=3.80; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121063 DONOR CAUSE OF DEATH:Cerebrovascular/Stroke RECOVERY DATE=8/26/2015; OPO=LAOP; ABO=B; AGE (years)=48; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=154.94; DONOR WEIGHT (kg)=119.15; Hep C Positive Donor=NO; Cold Ischemia (min)=207; Cold Ischemia (Hrs)=3.45; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 120237 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=8/5/2015; OPO=LAOP; ABO=O; AGE (years)=40; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=177.8; DONOR WEIGHT (kg)=82.8; Hep C Positive Donor=NO; Cold Ischemia (min)=320; Cold Ischemia (Hrs)=5.33; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 120240 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=8/6/2015; OPO=TNDS; ABO=AB; AGE (years)=27; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=183; DONOR WEIGHT (kg)=93.3; Hep C Positive Donor=NO; Cold Ischemia (min)=293; Cold Ischemia (Hrs)=4.88; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 120257 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=8/15/2015; OPO=ALOB; ABO=O; AGE (years)=28; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=183; DONOR WEIGHT (kg)=78; Hep C Positive Donor=YES; Cold Ischemia (min)=226; Cold Ischemia (Hrs)=3.77; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121095 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=9/22/2015; OPO=LAOP; ABO=O; AGE (years)=23; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=170.18; DONOR WEIGHT (kg)=61.37; Hep C Positive Donor=NO; Cold Ischemia (min)=171; Cold Ischemia (Hrs)=2.85; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 114300 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=1/31/2015; OPO=LAOP; ABO=A; AGE (years)=25; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=66.22; Hep C Positive Donor=NO; Cold Ischemia (min)=377; Cold Ischemia (Hrs)=6.28; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 116736 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=4/11/2015; OPO=LAOP; ABO=B; AGE (years)=25; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=139.7; DONOR WEIGHT (kg)=84.14; Hep C Positive Donor=NO; Cold Ischemia (min)=281; Cold Ischemia (Hrs)=4.68; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121087 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=9/18/2015; OPO=LAOP; ABO=O; AGE (years)=26; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=175.26; DONOR WEIGHT (kg)=74.84; Hep C Positive Donor=NO; Cold Ischemia (min)=366; Cold Ischemia (Hrs)=6.10; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 117323 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=6/8/2015; OPO=LAOP; ABO=O; AGE (years)=27; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=177.8; DONOR WEIGHT (kg)=67.8; Hep C Positive Donor=NO; Cold Ischemia (min)=262; Cold Ischemia (Hrs)=4.37; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121091 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=9/20/2015; OPO=LAOP; ABO=O; AGE (years)=31; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=172.72; DONOR WEIGHT (kg)=77.65; Hep C Positive Donor=NO; Cold Ischemia (min)=414; Cold Ischemia (Hrs)=6.90; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121089 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=9/19/2015; OPO=LAOP; ABO=O; AGE (years)=34; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=95; Hep C Positive Donor=NO; Cold Ischemia (min)=46; Cold Ischemia (Hrs)=0.77; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 116709 DONOR CAUSE OF DEATH:Trauma RECOVERY DATE=2/17/2015; OPO=LAOP; ABO=O; AGE (years)=35; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=72.57; Hep C Positive Donor=NO; Cold Ischemia (min)=367; Cold Ischemia (Hrs)=6.12; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 114647 DONOR CAUSE OF DEATH:Cerebrovascular/Stroke RECOVERY DATE=2/12/2015; OPO=LAOP; ABO=O; AGE (years)=39; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=170.18; DONOR WEIGHT (kg)=80.83; Hep C Positive Donor=NO; Cold Ischemia (min)=156; Cold Ischemia (Hrs)=2.60; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 118601 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=7/19/2015; OPO=LAOP; ABO=A1; AGE (years)=39; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=154.9; DONOR WEIGHT (kg)=62.5; Hep C Positive Donor=NO; Cold Ischemia (min)=236; Cold Ischemia (Hrs)=3.93; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121079 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=9/8/2015; OPO=LAOP; ABO=O; AGE (years)=40; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=160; DONOR WEIGHT (kg)=67.2; Hep C Positive Donor=NO; Cold Ischemia (min)=338; Cold Ischemia (Hrs)=5.63; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121364 DONOR CAUSE OF DEATH:CVA RECOVERY DATE=10/7/2015; OPO=LAOP; ABO=O; AGE (years)=43; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=172.72; DONOR WEIGHT (kg)=82.9; Hep C Positive Donor=NO; Cold Ischemia (min)=54; Cold Ischemia (Hrs)=0.90; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121358 DONOR CAUSE OF DEATH:CVA RECOVERY DATE=10/4/2015; OPO=LAOP; ABO=O; AGE (years)=44; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=162.6; DONOR WEIGHT (kg)=53.9; Hep C Positive Donor=NO; Cold Ischemia (min)=246; Cold Ischemia (Hrs)=4.10; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 121356 DONOR CAUSE OF DEATH:CVA RECOVERY DATE=10/3/2015; OPO=LAOP; ABO=O; AGE (years)=49; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=167.6; DONOR WEIGHT (kg)=52.4; Hep C Positive Donor=NO; Cold Ischemia (min)=336; Cold Ischemia (Hrs)=5.60; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO SUBJECT_SAMPLE_FACTORS - 115347 DONOR CAUSE OF DEATH:Anoxia RECOVERY DATE=4/23/2015; OPO=LAOP; ABO=B; AGE (years)=49; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=162.56; DONOR WEIGHT (kg)=73.84; Hep C Positive Donor=NO; Cold Ischemia (min)=280; Cold Ischemia (Hrs)=4.67; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO #COLLECTION CO:COLLECTION_SUMMARY A wedge biopsy of the right lobe of the liver is routinely obtained prior to CO:COLLECTION_SUMMARY transplantation. The sample is immediately flash frozen in the operating room CO:COLLECTION_SUMMARY and subsequently transferred to Ochsner Biobank for sample storage. CO:SAMPLE_TYPE Liver Tissue CO:COLLECTION_METHOD Open biopsy during cold storage preservation of organ for transplant CO:COLLECTION_LOCATION Operating room of liver transplant during CO:COLLECTION_FREQUENCY Single sample prior to transplant CO:COLLECTION_DURATION Not applicable CO:COLLECTION_TIME Not applicable CO:VOLUMEORAMOUNT_COLLECTED ~50mg CO:STORAGE_CONDITIONS -180 degree C, liquid nitrogen vapor phase CO:COLLECTION_VIALS ThermoScientific CryoTube vials, 1.8mL CO:STORAGE_VIALS ThermoScientific CryoTube vials, 1.8mL CO:COLLECTION_TUBE_TEMP -70 degree liguid nitrogen flash freezing CO:ADDITIVES None CO:BLOOD_SERUM_OR_PLASMA N/A CO:TISSUE_CELL_IDENTIFICATION N/A CO:TISSUE_CELL_QUANTITY_TAKEN N/A #TREATMENT TR:TREATMENT_SUMMARY Not Applicable TR:TREATMENT_PROTOCOL_ID Not Applicable TR:TREATMENT_PROTOCOL_FILENAME Not Applicable TR:TREATMENT_PROTOCOL_COMMENTS Not Applicable TR:TREATMENT Not Applicable TR:TREATMENT_COMPOUND Not Applicable TR:TREATMENT_ROUTE Not Applicable TR:TREATMENT_DOSE Not Applicable TR:TREATMENT_DOSEVOLUME Not Applicable TR:TREATMENT_DOSEDURATION Not Applicable TR:TREATMENT_VEHICLE Not Applicable #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Amino acid assay|CoA assay|NAD-ATP assay|NADH assay|Organic acid assay SP:SAMPLEPREP_PROTOCOL_ID SB_AA_Assay.pdf;SB_CoA_Assay.pdf;SB_NAD-ATP_Assay_Agilent.pdf;SB_NADH_Assay_Agilent.pdf;SB_OA_Assay.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY CoA assay CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2mm, 1.7um) CH:METHODS_FILENAME SB_CoA_Assay.pdf #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS CoA assay-ESI #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg dry powder MS_METABOLITE_DATA_START Samples 114291 116707 115165 115333 115342 117751 Factors DONOR CAUSE OF DEATH:Anoxia DONOR CAUSE OF DEATH:Trauma DONOR CAUSE OF DEATH:Anoxia DONOR CAUSE OF DEATH:Cerebrovascular/Stroke DONOR CAUSE OF DEATH:Trauma DONOR CAUSE OF DEATH:Trauma Acetyl-CoA 14.43 19.18 12.09 29.49 30.54 42.42 Malonyl-CoA 1.93 1.39 1.21 0.99 1.60 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID Acetyl-CoA 1.37 810.1 444493 Malonyl-CoA 1.16 854.1 644066 METABOLITES_END #END