#METABOLOMICS WORKBENCH araskind_20160414_082440 DATATRACK_ID:589 STUDY_ID:ST000377 ANALYSIS_ID:AN000611 PROJECT_ID:PR000296
VERSION             	1
CREATED_ON             	April 19, 2016, 4:06 pm
#PROJECT
PR:PROJECT_TITLE                 	Role of kidney acetylcholine in protection from kidney injury
PR:PROJECT_SUMMARY               	ChAT was induced in a model of kidney inflammation to determine its role in
PR:PROJECT_SUMMARY               	kidney protection
PR:INSTITUTE                     	University of Colorado, Denver
PR:DEPARTMENT                    	Medicine
PR:LABORATORY                    	Garcia Lab
PR:LAST_NAME                     	Garcia
PR:FIRST_NAME                    	Gabriela
PR:ADDRESS                       	Denver, CO
PR:EMAIL                         	Gabriela.garcia@ucdenver.edu
PR:PHONE                         	303-724-4897
#STUDY
ST:STUDY_TITLE                   	Kidney choline acetyltransferase (ChAT)-(part II)
ST:STUDY_TYPE                    	Regular
ST:STUDY_SUMMARY                 	Rats were treated with donepezil (DPZ) after the induction of a model of
ST:STUDY_SUMMARY                 	glomerulonephritis (GN) to increase kidney ACh levels. Rats were euthanized at
ST:STUDY_SUMMARY                 	day 25 after the induction of the disease. Kidneys were rapidly removed, frozen
ST:STUDY_SUMMARY                 	in liquid nitrogen and stored at -80C
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	4
ST:TOTAL_SUBJECTS                	19
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10114
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018110	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018111	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018112	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018113	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018114	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018115	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018116	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018117	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018118	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018119	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018120	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018121	Disease:GN | Treated:Treated	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018122	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018123	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018124	Disease:GN | Treated:Vehicle	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018125	Disease:Nl | Treated:Control	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018126	Disease:Nl | Treated:Control	
SUBJECT_SAMPLE_FACTORS           	SU0012168	S00018127	Disease:Nl | Treated:Control	
#COLLECTION
CO:COLLECTION_SUMMARY            	none
CO:SAMPLE_TYPE                   	Pooled sample
#TREATMENT
TR:TREATMENT_SUMMARY             	none
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	none
SP:SAMPLEPREP_PROTOCOL_FILENAME  	2014-10-07-Neurotransmitter.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	None
CH:INSTRUMENT_NAME               	None
CH:COLUMN_NAME                   	Cogent Diamond Hydride
CH:METHODS_FILENAME              	QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip
CH:METHODS_ID                    	DQM0150
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6410A QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	pmol/mg sample
MS_METABOLITE_DATA_START
	S00018110	S00018111	S00018112	S00018113	S00018114	S00018115	S00018116	S00018117	S00018118	S00018119	S00018120	S00018121	S00018122	S00018123	S00018124	S00018125	S00018126	S00018127
5-Hydroxyindoleacetic acid	19.22	24.94	5.13	112.61	3.92	12.60	7.41	5.21	10.86	6.13	11.50	1.16	10.51	7.46	7.60	8.62	5.95	4.42
Adenosine	170.10	54.01	56.95	329.77	53.05	76.37	108.84	9.07	86.77	64.97	73.21	75.46	28.41	19.39	51.67	60.07	41.15	52.57
Serotonin	1.55	1.50	1.55	1.51	1.57	1.50	1.56	1.83	1.53	1.59	1.64		1.53	1.42	1.61	1.58	1.54	1.48
3-Methoxytyramine	0.76	0.76	0.76	0.76	0.76	0.75	0.76	0.76	0.75	0.76	0.76		0.76	0.76	0.76	0.76	0.76	0.76
Acetylcholine				3.33										0.19				0.18
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	retention index	quantified m/z	PubChem ID	KEGG ID
5-Hydroxyindoleacetic acid			1826
Adenosine			60961
Serotonin			5202
3-Methoxytyramine			1669
Acetylcholine			187
METABOLITES_END
#END