#METABOLOMICS WORKBENCH michaelsa93_20160701_111053_mwtab.txt DATATRACK_ID:661
VERSION             	1
CREATED_ON             	November 15, 2016, 8:44 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolic profiling during ex vivo machine perfusion of the human liver
PR:PROJECT_SUMMARY               	As donor organ shortages persist, functional machine perfusion is under
PR:PROJECT_SUMMARY               	investigation to improve preservation of the donor liver. The transplantation of
PR:PROJECT_SUMMARY               	donation after circulatory death (DCD) livers is limited by poor outcomes, but
PR:PROJECT_SUMMARY               	its application may be expanded by ex vivo repair and assessment of the organ
PR:PROJECT_SUMMARY               	before transplantation. Here we employed subnormothermic (21 °C) machine
PR:PROJECT_SUMMARY               	perfusion of discarded human livers combined with metabolomics to gain insight
PR:PROJECT_SUMMARY               	into metabolic recovery during machine perfusion. Improvements in energetic
PR:PROJECT_SUMMARY               	cofactors and redox shifts were observed, as well as reversal of
PR:PROJECT_SUMMARY               	ischemia-induced alterations in selected pathways, including lactate metabolism
PR:PROJECT_SUMMARY               	and increased TCA cycle intermediates. We next evaluated whether DCD livers with
PR:PROJECT_SUMMARY               	steatotic and severe ischemic injury could be discriminated from
PR:PROJECT_SUMMARY               	‘transplantable’ DCD livers. Metabolomic profiling was able to cluster
PR:PROJECT_SUMMARY               	livers with similar metabolic patterns based on the degree of injury. Moreover,
PR:PROJECT_SUMMARY               	perfusion parameters combined with differences in metabolic factors suggest
PR:PROJECT_SUMMARY               	variable mechanisms that result in poor energy recovery in injured livers. We
PR:PROJECT_SUMMARY               	conclude that machine perfusion combined with metabolomics has significant
PR:PROJECT_SUMMARY               	potential as a clinical instrument for the assessment of preserved livers.
PR:INSTITUTE                     	University of California, Davis
PR:DEPARTMENT                    	Genome and Biomedical Sciences Facility
PR:LABORATORY                    	WCMC Metabolomics Core
PR:LAST_NAME                     	Fiehn
PR:FIRST_NAME                    	Oliver
PR:ADDRESS                       	1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis,
PR:ADDRESS                       	CA 95616
PR:EMAIL                         	ofiehn@ucdavis.edu
PR:PHONE                         	(530) 754-8258
PR:FUNDING_SOURCE                	NIH U24DK097154
PR:PUBLICATIONS                  	Metabolic profiling during ex vivo machine perfusion of the human liver
PR:DOI                           	10.1038/srep22415
#STUDY
ST:STUDY_TITLE                   	Metabolic profiling during ex vivo machine perfusion of the human liver (part
ST:STUDY_TITLE                   	III)
ST:STUDY_SUMMARY                 	As donor organ shortages persist, functional machine perfusion is under
ST:STUDY_SUMMARY                 	investigation to improve preservation of the donor liver. The transplantation of
ST:STUDY_SUMMARY                 	donation after circulatory death (DCD) livers is limited by poor outcomes, but
ST:STUDY_SUMMARY                 	its application may be expanded by ex vivo repair and assessment of the organ
ST:STUDY_SUMMARY                 	before transplantation. Here we employed subnormothermic (21 °C) machine
ST:STUDY_SUMMARY                 	perfusion of discarded human livers combined with metabolomics to gain insight
ST:STUDY_SUMMARY                 	into metabolic recovery during machine perfusion. Improvements in energetic
ST:STUDY_SUMMARY                 	cofactors and redox shifts were observed, as well as reversal of
ST:STUDY_SUMMARY                 	ischemia-induced alterations in selected pathways, including lactate metabolism
ST:STUDY_SUMMARY                 	and increased TCA cycle intermediates. We next evaluated whether DCD livers with
ST:STUDY_SUMMARY                 	steatotic and severe ischemic injury could be discriminated from
ST:STUDY_SUMMARY                 	‘transplantable’ DCD livers. Metabolomic profiling was able to cluster
ST:STUDY_SUMMARY                 	livers with similar metabolic patterns based on the degree of injury. Moreover,
ST:STUDY_SUMMARY                 	perfusion parameters combined with differences in metabolic factors suggest
ST:STUDY_SUMMARY                 	variable mechanisms that result in poor energy recovery in injured livers. We
ST:STUDY_SUMMARY                 	conclude that machine perfusion combined with metabolomics has significant
ST:STUDY_SUMMARY                 	potential as a clinical instrument for the assessment of preserved livers.
ST:INSTITUTE                     	University of California, Davis
ST:DEPARTMENT                    	Genome and Biomedical Sciences Facility
ST:LABORATORY                    	WCMC Metabolomics Core
ST:LAST_NAME                     	Fiehn
ST:FIRST_NAME                    	Oliver
ST:ADDRESS                       	1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis,
ST:ADDRESS                       	CA 95616
ST:EMAIL                         	ofiehn@ucdavis.edu
ST:PHONE                         	(530) 754-8258
ST:STUDY_COMMENTS                	The first 4 samples were a test run to see how efficient the analysis was and
ST:STUDY_COMMENTS                	were run on a lipidomics platform. The next 12 samples were the used in the
ST:STUDY_COMMENTS                	paper and were the same as the original 4 samples, but they were split into 3
ST:STUDY_COMMENTS                	biological replicates and run on the GC platform.
ST:PUBLICATIONS                  	doi:10.1038/srep22415
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	23.0.A_01	Inj09_SA01_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	23.0.B_02	Inj22_SA02_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	23.0.C_03	Inj14_SA03_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	23.1.A_04	Inj06_SA04_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	23.1.B_05	Inj10_SA05_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	23.1.C_06	Inj20_SA06_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	23.2.A_07	Inj01_SA07_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	23.2.B_08	Inj05_SA08_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	23.2.C_09	Inj17_SA09_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	23.3.A_10	Inj12_SA10_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	23.3.B_11	Inj24_SA11_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	23.3.C_12	Inj21_SA12_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	31.0.A_13	Inj04_SA13_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	31.0.B_14	Inj02_SA14_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	31.0.C_15	Inj11_SA15_BruinCSH.d	Collection Time:-	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	31.1.A_16	Inj15_SA16_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	31.1.B_17	Inj23_SA17_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	31.1.C_18	Inj03_SA18_BruinCSH.d	Collection Time:1 Hr	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	31.2.A_19	Inj13_SA19_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	31.2.B_20	Inj08_SA20_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	31.2.C_21	Inj19_SA21_BruinCSH.d	Collection Time:2 Hr	Treatment=steatotic liver; Replicate=3
SUBJECT_SAMPLE_FACTORS           	31.3.A_22	Inj16_SA22_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=1
SUBJECT_SAMPLE_FACTORS           	31.3.B_23	Inj18_SA23_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=2
SUBJECT_SAMPLE_FACTORS           	31.3.C_24	Inj07_SA24_BruinCSH.d	Collection Time:3 Hr	Treatment=steatotic liver; Replicate=3
#COLLECTION
CO:COLLECTION_SUMMARY            	Liver biopsies were snap frozen in liquid nitrogen and stored at ­80. Tissue
CO:COLLECTION_SUMMARY            	was pulverized, while frozen and tissue was aliquoted into .6 ml tubes between
CO:COLLECTION_SUMMARY            	30 and 70 mg of liver tissue. 3 replicates are provided per sample.
CO:COLLECTION_PROTOCOL_FILENAME  	StudyDesign_BoteBruinsma_072414.pdf
CO:SAMPLE_TYPE                   	Tissue
CO:STORAGE_CONDITIONS            	-80 C
CO:TISSUE_CELL_QUANTITY_TAKEN    	between 30 and 70 mg
#TREATMENT
TR:TREATMENT_SUMMARY             	2 livers (names 23 and 31) were perfused for 3 hours, providing 4 time­course
TR:TREATMENT_SUMMARY             	(0, 1, 2, 3) biopsies. 3 replicates for each biopsy (A, B ,C). These were the
TR:TREATMENT_SUMMARY             	only 2 run on the Lipidomics platform.
TR:TREATMENT_PROTOCOL_FILENAME   	StudyDesign_BoteBruinsma_072414.pdf
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	1. Weigh 4mg tissue sample in to a 2mL Eppendorf tube. 2. Add 1mL extraction
SP:SAMPLEPREP_SUMMARY            	solvent to the tissue sample and homogenize for 45 seconds ensuring that sample
SP:SAMPLEPREP_SUMMARY            	resembles a powder. In between samples, clean the homogenizer in solutions of
SP:SAMPLEPREP_SUMMARY            	methanol, acetone, water, and the extraction solvent in the order listed. 3.
SP:SAMPLEPREP_SUMMARY            	Vortex samples for 10 seconds, then 5 minutes on 4°C shaker. 4. Centrifuge the
SP:SAMPLEPREP_SUMMARY            	samples for 2 minutes at 14,000 rcf. Aliquot 500μL supernatant for analysis,
SP:SAMPLEPREP_SUMMARY            	and 500μL for a backup. Store backup aliquots in the -20°C freezer. 5.
SP:SAMPLEPREP_SUMMARY            	Evaporate one 500μl analysis aliquot in the Labconco Centrivap cold trap
SP:SAMPLEPREP_SUMMARY            	concentrator to complete dryness (typically overnight). 6. The dried aliquot is
SP:SAMPLEPREP_SUMMARY            	then re-suspended with 500l 50% acetonitrile (degassed as given) 7.
SP:SAMPLEPREP_SUMMARY            	Centrifuge for 2 minutes at 14,000 rcf using the centrifuge Eppendorf 5415. 8.
SP:SAMPLEPREP_SUMMARY            	Remove supernatant to a new Eppendorf tube. 9. Evaporate the supernatant to
SP:SAMPLEPREP_SUMMARY            	dryness in the the Labconco Centrivap cold trap concentrator. 10. Submit to
SP:SAMPLEPREP_SUMMARY            	derivatization.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	SOP_Extraction_of_Liver_Tissue_Samples.pdf
SP:PROCESSING_METHOD             	Homogenization
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 6550
CH:COLUMN_NAME                   	Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)
CH:COLUMN_NAME                   	1.7um Pre-Column
CH:FLOW_GRADIENT                 	15% B to 99%B
CH:FLOW_RATE                     	0.6 mL/min
CH:COLUMN_TEMPERATURE            	65 C
CH:METHODS_FILENAME              	Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
CH:SOLVENT_A                     	60:40 Acetonitrile:Water +10mM Ammonium Acetate +10mM Acetic Acid
CH:SOLVENT_B                     	9:1 Isopropanol:Acetonitrile +10mM Ammonium Acetate +10mM Acetic Acid
CH:COLUMN_PRESSURE               	450-850 bar
CH:INTERNAL_STANDARD             	See data dictionary
CH:RETENTION_TIME                	See data dictionary
CH:SAMPLE_INJECTION              	5 uL
CH:ANALYTICAL_TIME               	13 min
CH:CAPILLARY_VOLTAGE             	3500 V
CH:TIME_PROGRAM                  	15 min
CH:WEAK_WASH_SOLVENT_NAME        	Isopropanol
CH:STRONG_WASH_SOLVENT_NAME      	Isopropanol
CH:TARGET_SAMPLE_TEMPERATURE     	Autosampler temp 4 C
CH:RANDOMIZATION_ORDER           	Excel generated
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	WCMC Metabolomics Core
AN:SOFTWARE_VERSION              	MassHunter
AN:DATA_FORMAT                   	.d
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:CAPILLARY_VOLTAGE             	3500 V
MS:COLLISION_GAS                 	Nitrogen
MS:DRY_GAS_FLOW                  	13 L/min
MS:DRY_GAS_TEMP                  	200 C
MS:FRAGMENT_VOLTAGE              	175 V
MS:FRAGMENTATION_METHOD          	Auto MS/MS
MS:ION_SOURCE_TEMPERATURE        	325 C
MS:ION_SPRAY_VOLTAGE             	1000 V
MS:IONIZATION                    	Neg
MS:PRECURSOR_TYPE                	Intact Molecule
MS:REAGENT_GAS                   	Nitrogen
MS:SOURCE_TEMPERATURE            	325 C
MS:DATAFORMAT                    	.d
MS:DESOLVATION_GAS_FLOW          	11 L/min
MS:DESOLVATION_TEMPERATURE       	350 C
MS:NEBULIZER                     	35 psig
MS:OCTPOLE_VOLTAGE               	750 V
MS:RESOLUTION_SETTING            	extended dynamic range
MS:SCAN_RANGE_MOVERZ             	60-1700 Da
MS:SCANNING_CYCLE                	2 Hz
MS:SCANNING_RANGE                	60-1700 Da
MS:SKIMMER_VOLTAGE               	65 V
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	counts
MS_METABOLITE_DATA_START
Samples	Inj09_SA01_BruinCSH.d	Inj22_SA02_BruinCSH.d	Inj14_SA03_BruinCSH.d	Inj06_SA04_BruinCSH.d	Inj10_SA05_BruinCSH.d	Inj20_SA06_BruinCSH.d	Inj01_SA07_BruinCSH.d	Inj05_SA08_BruinCSH.d	Inj17_SA09_BruinCSH.d	Inj12_SA10_BruinCSH.d	Inj24_SA11_BruinCSH.d	Inj21_SA12_BruinCSH.d	Inj04_SA13_BruinCSH.d	Inj02_SA14_BruinCSH.d	Inj11_SA15_BruinCSH.d	Inj15_SA16_BruinCSH.d	Inj23_SA17_BruinCSH.d	Inj03_SA18_BruinCSH.d	Inj13_SA19_BruinCSH.d	Inj08_SA20_BruinCSH.d	Inj19_SA21_BruinCSH.d	Inj16_SA22_BruinCSH.d	Inj18_SA23_BruinCSH.d	Inj07_SA24_BruinCSH.d
Factors	Collection Time:-	Collection Time:-	Collection Time:-	Collection Time:1 Hr	Collection Time:1 Hr	Collection Time:1 Hr	Collection Time:2 Hr	Collection Time:2 Hr	Collection Time:2 Hr	Collection Time:3 Hr	Collection Time:3 Hr	Collection Time:3 Hr	Collection Time:-	Collection Time:-	Collection Time:-	Collection Time:1 Hr	Collection Time:1 Hr	Collection Time:1 Hr	Collection Time:2 Hr	Collection Time:2 Hr	Collection Time:2 Hr	Collection Time:3 Hr	Collection Time:3 Hr	Collection Time:3 Hr
5.06_554.48						5377	5673	5086				5260				5973	7454	6966	6301	5349	5980	8236	7785	7922
5.98_584.53	5525	5542	6622	6667	6881	7344	8374	8109	7252	6801	7181	8264					4740					5863	5271	
5.73_572.48_5.74_582.51	60835	62517	71129	67422	66534	91486	96671	88626	79771	81412	81896	99776	77600	78944	70235	108546	131645	136945	126341	109419	116195	170385	155177	148250
5.15_580.49				4034		5621	5554	5216	4467		5030	5524				6922	7456	6822	6563	5213	5500	7741	7741	7229
6.44_610.54	19831	19091	22424	24351	24374	30098	33191	28507	27159	23156	26450	31855	16161	17718	15358	21057	30525	27154	33131	25073	25722	38380	31229	31407
7.17_628.54_7.14_638.57	26036	25968	30610	29086	30399	37489	41418	37167	32036	29818	31018	37814	29699	31116	25783	39259	47572	46811	51374	37856	42451	60800	55770	54058
7.5_652.59	4922	4531	6062	5453	5072	6169	7755	6477	6283	5211	5731	6830	8629	8648	6934	8469	11437	10481	13425	9527	10537	15857	13428	14003
7.82_656.58_7.79_666.6	87742	88212	99207	96167	93272	104908	119378	111181	99376	88637	98444	108996	109191	108808	94597	106994	131970	128057	149411	112638	124541	167388	147795	148770
7.24_664.59	9475	9147	10853	10905	10474	12752	14322	12786	10105	9792	10950	13095	12444	12720	10937	13917	19472	16894	21658	15113	17773	25049	20095	20267
8.14_670.59_8.13_680.62	58579	58104	66740	59417	63450	67476	77740	71691	66571	59027	63141	72827	81729	86846	71703	76258	94782	93492	112370	83137	89537	116759	106537	110919
8.45_694.64_8.45_684.61	149891	150307	169894	159697	161926	172540	194557	179925	174478	145829	161880	177207	177310	178446	157843	147893	189709	181088	222578	170821	182258	232160	208313	209551
7.77_692.62	50680	52433	60689	61990	61029	68339	78483	70552	61325	60082	63805	71271	92475	91446	76100	90521	114402	106139	134891	100613	108221	153785	130882	131644
9.15_1447.96	21992	23143	33881	24986	24595	21342	26928	23992	21552	17825	21014	20062	28664	28917	23938	23831	32493	30973	29992	20268	23044	29276	25046	29056
3.29_281.25	30563	28682	41709	22837	26912	33070	47063	44207	41281	26250	29038	37818	9200	9438	8629	11666	14477	14045	11852	7513	10529	15226	12571	10806
2.9_279.23	12731	12054	20108	8981	10681	17015	17905	18133	18865	8860	9795	15697	4768	4441	3282	7027	8311	8101	5394	3843	5253	8992	6682	5454
2.8_303.23	16601	16535	24675	18033	20473	26541	20797	22432	18106	12985	14781	23460	8468	8093	6562	13169	16462	17730	11379	6798	9049	17126	11064	8131
7.76_856.69	4185	4442	4372	4537	3921	4052	4658	4132	3809	4160	4203	3728	6973	6237	5977	5924	5475	5158	5303	4701	4480	4966	4908	4753
1.58_540.33	23648	22458	25232	15523	18713	13438	22255	22472	23892	11658	17494	15968	28089	27639	24588	4913	6884	6639	9462	5320	5572	8078	5395	7071
1.25_564.33	3000	3520	4091		2472	2476	4217	3807	4178		2256	3491	2026	2219	2090									
1.62_452.28	13184	12861	16035	8338	10230	8564	12755	14927	12349	7336	9150	9706	19323	18355	17495	5990	7587	6812	7417		4768	9925		7263
4.92_750.53	9195	8607	10191	9251	9460	9774	10242	8702	8267	6718	7570	8407	11259	11309	9783	6307	8485	8859	11582	8927	8475	9826	7759	9477
5.53_778.56	69395	66980	75041	71015	72126	72989	72741	64467	64286	51904	54810	57917	58337	59511	55363	46769	57918	54264	58998	50008	49905	50758	46902	48508
4.99_776.54	45799	44826	50231	47474	49215	50008	54245	49392	49285	41231	45458	47286	34869	36186	30385	22813	28134	25477	34876	30223	29347	32637	30187	32229
4.53_774.53	9440	9522	11452	10012	11145	11077	14519	12032	12976	10212	11326	12130	12467	13393	12585	8441	9611	9302	14141	12331	11606	12981	11572	12038
4.81_788.54	7199	6691	7533	7648	7413	7354	8260	7966	7820	6327	6953	7525	8601	8769	7817	6442	7914	7188	10635	8914	7642	8424	7988	8733
6.21_806.59	9818	10552	11341	10415	10010	11463	10566	11017	9836	8402	8978	8988	7669	8118	7332	6569	8142	7599	9069	7654	7382	7357	7082	7008
5.61_804.58	387278	389158	433683	421771	418356	418425	450673	414573	418789	357306	386538	393827	362597	372461	321170	281350	349721	323350	421372	349977	361245	364715	327702	338756
5.13_802.56_5.13_870.55	573772	576928	652628	593435	619923	597875	665530	621527	604465	554253	564939	566989	659742	654491	585948	548229	619293	596171	707233	606520	615640	632838	575606	592277
5.42_816.58	10428	10103	11171	10451	10973	11183	12282	10857	10576	9341	9600	9674	11511	12064	10973	10099	11155	10938	13659	10959	10778	11756	11136	10811
6.28_832.61	60839	60146	70598	65413	65980	66068	73579	70399	68342	60279	67205	65452	62198	63489	54070	43772	57337	53616	70788	63008	62430	65585	57147	61827
5.77_830.59_5.77_898.58	231621	234006	255379	247172	251930	249620	278838	254084	256632	220564	233229	245528	291401	288358	261622	216496	262519	241805	299575	256788	259016	277152	252794	261110
5.3_828.58	81524	80345	89176	85111	84486	84770	93110	84835	87133	73983	77155	79667	80825	78534	71503	61428	70634	66896	85829	71544	73897	79135	72593	72755
5.04_826.56	272016	270853	302106	273799	279829	283944	314776	287627	287219	256920	272894	281903	224946	223232	194929	177436	220422	201904	251406	217302	207368	221773	201148	209510
4.64_824.54	50489	51766	57790	54763	53568	53072	61423	55866	54161	44888	49709	50301	66487	69103	57869	46776	59852	56248	75420	60671	60705	64814	57829	58786
5.95_856.61	21680	22207	23653	23590	23868	23878	25056	24117	22851	19945	22551	22502	23857	24705	21540	16193	19378	18810	25339	20981	21729	24337	20817	21634
5.69_854.59	101305	98688	110620	104797	107092	108073	115833	107422	104508	90866	100576	102441	86071	84907	73073	62904	76526	71893	91738	75486	75270	81481	74672	76764
5.08_852.58	28338	28107	29278	28331	29256	29635	30870	30897	28770	26257	27903	29588	21284	21954	19701	18825	21707	21446	26246	23464	23166	22509	20201	21050
4.86_850.56	120502	125800	138670	130132	126164	130480	143783	132789	130197	110543	119993	122954	114562	118221	102829	90749	110288	104291	155155	121064	127903	126471	113752	119562
6.14_882.62	4175	4060	4363	4293	4396	4510	4744	4491	4653	3816	4239	4142	2326	2042					2471		2342	2296		
5.69_880.61	7648	6879	7634	8317	7237	7655	8209	8034	7458	6442	7559	7533	5812	5405	5370	4275	4966	4660	6588	5873	5215	5361	5394	5335
5.5_878.59	35301	34701	38064	36562	37817	35589	41519	36618	36955	30554	33653	34412	33593	33207	30330	24235	29777	27524	40640	32658	35020	36211	32727	34526
5.78_716.52	144980	144251	166454	154738	159513	154734	167973	158421	154815	122776	137341	137833	115188	107464	91519	71807	97549	87454	86599	74046	72380	84281	71027	80095
5.31_714.51	240133	242801	273711	263657	271142	269743	306130	273006	285989	239770	267891	265268	234008	235917	213615	182796	225594	209124	229202	196011	193975	223036	197623	209404
5.14_742.54	6496	6680	6938	6936	7049	7016	7442	6743	7020	6238	6999	6691	7894	7698	6531	6292	6925	6814	8097	6907	6746	7291	6908	6656
5.97_742.54	246910	244256	283066	256463	262002	259332	298183	278649	271772	232841	259712	255926	247562	256594	218665	195622	244825	233321	249161	215163	206472	242306	214358	222344
5.38_740.52	59273	56243	64899	60138	60347	60025	71375	70168	65080	60655	65187	66301	71633	72836	65045	71544	81845	77962	71104	60710	60157	65172	60822	64998
5.19_738.51	320243	309764	352223	326241	332918	334537	375549	352261	355076	317159	341246	352208	260171	265907	230106	218196	257483	246430	285925	253050	239016	257566	233288	251632
5.87_766.54	579343	572533	658825	601825	622486	630446	668468	616976	614646	537807	566408	569266	560875	562378	517875	463480	542829	532938	561381	505524	511500	542488	501323	534956
5.07_762.51	292036	283928	308447	296727	301370	308975	344500	305647	321022	271389	291880	306254	250401	257053	226245	185085	229563	208140	268407	227337	216201	230512	206242	219197
5.37_840.58	4683	4310	5018	4838	4453	4668	4750	4764	4264	4186	4222	4433	3605	3684	3734	3144	3559	3225	4067	3693	3548	3699	3251	3462
5.26_764.52	103683	105370	112102	109636	109734	114425	126259	114498	112171	103374	108478	108648	67105	68245	60173	58148	70725	64764	75104	65325	62773	61868	59503	59751
5.1_747.52	30169	28572	32702	30689	31262	31359	35243	31337	33806	26269	30116	30566	41024	43287	34519	31240	40813	37383	43231	34642	35694	41907	37174	37352
4.8_773.54	36848	36688	46617	38434	40387	36734	44689	39079	37523	31435	36284	38069	128177	129668	104536	75357	107374	91055	129785	94772	92139	118347	96294	102992
4.37_771.52	28314	27733	35695	28926	30151	27623	32401	32525	28863	24924	28347	29886	118174	120579	101220	74683	100714	88500	116285	90755	89916	110078	92935	105648
4.01_769.5	8612	7533	9716	8151	8821	8406	8509	8916	8285	6676	7882	8294	31698	33152	27082	20702	27223	24357	31355	24458	23890	30638	26331	30274
4.19_819.52	15271	14102	17070	14147	13261	13176	15114	13518	13086	11122	12191	12555	61245	62341	49818	40077	56739	49875	63620	46079	46178	53526	45469	51079
3.86_817.5	10633	9983	10477	8857	8932	8485	10096	8653	8370	7236	8366	8025	38076	39320	30491	27387	35391	31415	37821	29979	30069	33714	29419	34565
4.39_807.5	13619	13926	14257	15938	15699	13728	15900	15307	14513	11682	14961	12620												
4.93_835.53	123520	128926	179183	162315	157540	136708	181091	155444	139850	92649	122640	120359	196534	192738	133683	100263	189719	161151	191755	108969	124001	181612	142860	170419
4.51_833.52	40833	43029	44228	47449	46952	41868	44994	43914	42265	34049	41446	36857	32634	33353	26836	30376	40355	35471	25938	23558	23449	30157	29519	32147
5.53_863.56	21623	23144	25666	23052	23913	21666	24833	24689	23250	21019	23444	19849	11013	11161	9718	11487	13356	13623	9280	8512	9692	12678	12013	12015
5.09_861.55	180975	193130	209010	199414	194658	167069	191123	193126	177623	146289	167140	152857	118284	116672	97840	108968	133834	121435	99869	88142	100465	132401	128322	138005
4.61_859.53	5593	5768	6111	7379	6551	6015	6561	7070	6437	5444	6823	5418	7957	8037	7084	10003	12426	10995	10197	8973	9912	8939	9127	9329
4.44_857.52	32506	32679	33863	39870	39221	34351	37934	37452	35404	29882	35145	31762	33474	32359	26729	29734	37609	32536	28201	24942	27228	31844	30626	32114
5.26_887.56	54983	62597	72399	66505	67159	56696	63680	63241	59126	42524	53165	44571	81793	78486	68559	62305	80211	73532	72444	54930	67850	98479	87153	90614
5.01_885.55	483697	513392	548486	535146	523776	468521	491553	516877	474459	385047	441212	395259	596839	569974	527809	496252	584523	551366	558799	481418	548009	622670	584248	599960
4.48_883.53	12710	13866	14928	14636	14729	12740	16351	17104	15510	13349	15911	14489	13276	12910	10930	12558	15727	14454	14134	11783	12314	13726	13987	13845
4.86_909.55	27608	30673	38965	33219	34075	28560	32757	32087	28614	22686	28005	25537	17938	17685	15006	14584	19301	18642	21875	14760	16326	21205	17764	19582
4.31_881.52	6647	7028	7564	6987	7201	6473	7167	7000	6892	5625	6686	6842	5929	5400	5183	5642	7365	7290	5614	4775	4991			5744
6.01_790.59	2199	5725	2834	2635	2376	2853	2436	2330	2377	2403	2252	2649	3487	3788	3702	3342	4011	3871	5434	4275	3854	4212	3525	4228
7.13_818.63	2539	2542	2883	2839	2890	2853	3094	3024	2350	2385	2476	2659	4618	4307	3876	2905	3589	3466	3423	2888	3030	3089	2946	3553
6.14_700.53					3663			4379					7346	7224	5942	5906	7958	6892	8767	8164	7783	6605	6364	6492
6.27_726.54	2343	2246	2534	2361	2549	2583	2410	2552	2215	2203	2381	2465	3823	3838	3417	2866	3956	3458	4659	3808	3834	3615	3184	3848
5.49_722.51	35796	32827	38958	38790	37709	41908	41062	39403	34808	34361	36941	40676	46215	49313	41180	35767	52889	45898	56210	44915	45057	45238	38628	40668
5.52_748.53	10133	10229	11082	11859	11153	12408	12029	11247	10506	10664	10731	10947	16279	16865	15220	13240	17690	16904	20246	17480	17272	15353	15113	14327
5.31_746.51	8483	8653	9546	9559	9717	9836	9854	8837	9302	8685	8630	9071	11954	11725	11106	9601	12249	11434	12971	11461	11542	11605	10230	10117
6.65_778.58	7220	7512	7916	8399	7268	8220	7884	8187	7210	7586	7986	8807	4599	5470			5576		6583	5640	6029	4915		
6.19_776.56	6135	6514	7766	7330	6934	7592	7435	7268	6649	6661	6757	7375	6696	7503	6120	5851	7847	7121	8869	6925	7410	6569	6142	6553
5.98_774.54	6446	5991	7104	7037	6296	7085	6715	6618	5729	6085	6362	6510	6860	7283	6338	5961	7552	7004	8655	7418	7205	6919	5992	6079
5.59_872.56	6082	5865	6005	6055	5431	5831	5446	5788	5787	5739	5883	6122	5970	6039	6028	5819	5730	6412	6136	5711	6185	5992	5506	5853
5.13_792.53	7685	7372	8014	7188	7305	7722	7754	7873	7373	7785	6725	7159	7758	8463	7640	7482	7337	8383	9057	8370	8218	7642	7790	8085
4.23_719.53	7443	6677	8841	7753	7944	8334	8342	8238	7341	6806	7072	7300	8749	9018	7381	6042	8279	7371	10580	7789	7896	7464	7220	7247
4.52_733.55	4282	4277	5349	4319	4588	4730	4920	4910	4436	3812	4163	4631	5385	5275	4709	3684	5014	4497	5658	4943	4958	4402	4004	4571
4.82_747.57	90891	87149	109128	99471	103492	100970	101273	98808	92930	89061	90739	96820	122515	122951	109695	89162	115490	107847	126605	114077	107510	107793	99433	103149
4.82_815.55	3432	3319	3686	3662	3524	3553	3688	3812	3596	3464	3310	3736	3999	4183	3798	3324	4020	4341	4228	3623	3823	3451	3150	4189
5.49_775.6	29604	28731	32228	32559	32523	33117	32876	31698	30769	27974	30515	30612	24176	24390	22108	18635	23000	20558	26284	23175	23307	20572	20977	20154
6.19_803.63	15785	16203	18793	17993	17260	17340	18322	17998	16736	14439	16094	16345	21219	21186	18193	15270	18372	17687	22584	19037	18672	18607	16565	17107
6.88_831.66	45221	43597	51093	47297	48012	47356	50930	48185	45310	41384	43009	45317	57161	58549	50049	40316	50789	47141	58846	48850	49061	50597	43177	46431
6.28_829.64	4833	5220	5440	5110	5124	4819	5243	5104	5126	4497	4772	4757	7688	8241	6694	5011	6461	5878	7629	7177	6633	6483	5707	6445
7.21_845.67	22443	20881	25345	22108	22809	22698	23355	23353	22455	19009	21208	22131	30504	32088	27474	21449	27903	27150	31282	25826	24870	27156	25126	25083
6.58_843.66	2455	2518	2985	2458	2625	2570	2630	2468	2691	2412	2529	2392	4622	4677	4083	2910	3850	3376	4759	3878	3698	3747	3359	3618
7.54_859.69	42087	41050	49596	44722	44042	44841	48233	44798	43788	37592	41157	40265	50689	51707	45780	37734	46532	43765	51079	43698	43986	44650	38714	41579
6.84_857.67	38830	36430	41276	40777	39284	38542	40691	39752	38113	34661	37077	38265	59962	60777	52478	44767	54503	50558	64317	56682	54400	52787	47813	52141
6.28_855.66	6507	6547	6978	6493	6896	7156	7200	7005	6764	5712	5888	6385	12526	13080	11485	8533	10819	10132	13351	11839	10961	10489	9636	10328
5.77_1616.18	6299	6620	7404	7187	7577	7567	8298	7567	7778	5837	6458	6692	9745	9073	7505	5513	8182	6614	9526	8067	7316	9149	7501	9375
6.54_817.64	4537	4553	5793	4811	5164	4571	5645	4879	4571	3986	4241	4226	7575	7836	6820	5461	6560	6408	8005	6506	6301	7064	5794	6387
8.12_697.61	10963	11070	11354	10811	10924	12018	12551	12934	11796	11215	11610	13335	14345	14602	12554	14057	15381	15329	18571	13827	14047	20349	18481	17094
8.45_711.63	20747	20990	21220	21264	19818	22064	23298	22634	22060	20874	22054	22277	22731	23126	21312	22026	24146	24451	25683	23433	24364	26772	25021	26440
2.36_568.36	10650	10521	12203	8114	10411	7029	11354	11509	11129	6087	8812	8630	13583	13230	10723	3283	4048	4181	5798	3509	2922	5193	3823	4495
4.94_325.18	2246	2534	2443	2566	2560	2548	2887	2703	2396	2302	2466	2531	4747	4992	4936	3984	4452	4070	5067	4527	5320	5001	3985	4301
5.61_1564.15	15150	15863	18262	16841		16805		16990	16697	12992	15335	15209	13656	14539	11545		12534	11298	16035	13243	13070	13426	11842	13141
5.77_1617.19	6306	6702	7350	7453	7369	7466	8820	7589	7825	6412	6479	6710	9520	9403	7654	5758	7854	6421	10242	7946	8049	9552	8097	8760
7.77_709.61	12312	12093	12723	13557	13214	13966	15808	14709	13641	12485	13501	13849	17088	16168	15536	16643	18247	18613	19172	17518	18697	20160	19561	20259
5.98_810.53	6369	6286				6203	6633	6597	6624	6653	6926	6317	6516	6995	5895	6140	6638	6614	5998	5906	6289	6055	5964	5960
12.14_119.04	7691	10471	6265	7970	7601	56696	9145	16990	10738	6207	8030	37659	8458	7064	7429	11665	16874	60467	6844	11088	10291	23888	8477	149975
13.76_119.04	60570	8894	4588	6271	15405	5890	8284	9470	7196	5090	54170	24909	6026	119017	5450	11563	9363	57348	9413	6744	7833	8888	7468	14274
0.52_151.03	5946	6777	4243	5185	4922	6171	6650	6193	7186		3971	5008	6742	8097	7721									
4.46_160.84	4303	4781	4451	4138	4534	4288	4923	4506	4731	4073	4627	4567	4146	4028	3824	3674	4673	4245	4005	3406	3613	4621	4320	3877
5.4_162.84	3447	3762	2930	2902	3018	3553	3030	2880	3194	3691	3861	3240	3342	3246	3950	4194	3774	3271	3184	3421	3707	3102	3980	3410
4.52_162.84	3570	4633	4115	4338	4106	4255	4154	4095	4647	4288	4135	4048	3575	3631	3345	3470	4676	3730	3498	2964	3413	3539	3866	3795
0.51_179.06	3887	4641	2001	7926	5776	7217	6344	5886	6260	4664	5238	5858	5977	7036	3950	3231	5280	3104	3351	3889	4334	3713	3951	4097
9.14_197.81	4457	4875	6340	5063	5108	4987	5661	5492	5129			4578	5607	5818	5312	5730	6142	6129	5761		5185	6456	5746	5855
11.36_230.05	8946	8950	8926	8930	9018	9074	9581	9045		8374	8631	8708	5974	5957	6330						4146	4745	4981	4716
10.65_230.05	9201	8751	9825	8884	8689	8704	9809	8682	8715	8462	8467	8687	8517	8897	8702	8413	8491	8717	8408	8607	8288	8432	8485	8487
4.97_231.93		4675	5414			4207							5671	5274	5146		5207	5259	5473	4445	5118	5021		4805
4.93_239.1	2112	2238	2351	2311	2264	2601	2577	2422	2312		2513	2554	3212	4048	3121	3116	3481	2870	3495	3319	3116	3445	3223	3294
0.73_307.1													45092	48810	39496	15307	16887	14756	31140	20399	21081	19285	22130	20647
4.93_311.17	2406	2529	2714	2776	2567	2452	2690	2602	2744	2561	2711	2803	5069	5390	5505	4303	4531	4095	5757	4506	5174	5272	4599	4992
1.56_313.14	3732	4204	3646	3890	4064	3247	4103	3918	3562	3387	3775	3985	3844	3994	3771	3333	2907	2962	3821	2996	3018	3450	3247	3386
1.57_323.17	3525	3608	3734	3198	4018	3315	4559	3953	3665	2906	3664	3390	4424	4858	4334	2782	3538	2436	3445	3040	2762	2801	3071	3167
2.66_327.23	9692	8179	13645	11232	11725	12219	12091	13980	11956	8005	10918	13971	10334	10940	10025	12614	14217	15160	8726	6148	7115	16724	13389	10733
4.93_367.24	7147	6229	6513	7025	6765	7197	8001	6619	6913	6605	6002	7106	10655	10889	10314	9199	9337	9312	11758	11800	11511	10796	10084	10207
4.38_446.38	7317	6333	8271	9094	6934	6882	5967	6594	5808	5188	5108	4381	12431	12339	10769	4560	5579	4908	5829	5837	4245	7176	5868	6428
3.18_465.3	11951	11176	12449	11741	12448	12297	14515	13844	12759	10423	14802	11723	19936	19939	19657	15693	19413	16642	22700	18286	22649	21670	21416	21434
4.91_479.35	8864	8904	8499	7859	8214	10136	9448	8359	9354	9916	10118	11335	11103	11440	11334	10588	11281	10174	12337	13208	12658	11948	10770	10971
2.41_480.31	22027	20808	29134	15340	17071	17331	21973	25802	21120	14145	16959	18652	30169	28927	25042	17533	22541	19883	16662	11693	10761	22432	13501	15933
3.19_505.34	11163	11253	13387	10680	12062	11697	13981	13132	12353	9595	12153	13243	11758	10432	9973	8030	9860	8799	12907	10137	11924	11088	10965	9727
4.93_505.35	2223	2745	2513	2306	2392	2854	3265	2481	2705	2769	3019	3036	4145	4449	4385	3477	3505	3596	5096	5207	4832	4234	4115	3479
5.39_558.47			4689		5040	5574	6976	6523		5967	5790	7100	6069	5655		8377	9266	9203	9107	6971	7605	11239	10417	10810
5.96_581.53	5048	5298	5983	5204	5873	5278	5241	5419	5276	5824	5177	5321	5659	5449	5441	5694	4977	5029	5361	5324	5388	5487	5157	4994
6.48_600.51	29262	28372	33574	32907	33863	38496	46057	39389	37288	33384	35509	41962	23887	25326	21801	30601	36410	36017	41356	32436	35032	46394	43283	44723
6.68_602.53	7909	8215	8809	11454	10601	10455	11485	10376	9501	9669	9473	10663										4892		4871
3.73_605.41	6771	6731	8139	6042	6873	6321	6664	6052	5447				9659	8530	8325	6236	7586		8308	6319	6890	7972	7337	6910
5.85_608.53	6616	6101	7257	8507	8591	9147	10233	10102	8248	7976	8825	10392	5135		4742	7980	9538	9519	10666	8566	8886	11449	10593	10365
6.44_627.53	6342	6433	7432	7312	7306	8158	9429	8907	8493	7736	7591	8999	4754	4738			7006	7177	7393	6849		8278	7763	8120
7.49_642.56	7706	8009	9014	8944	8327	10135	10613	10397	8853	9072	9625	11139	10569	10743	9155	14538	16509	16194	15599	12786	13549	18375	16191	17777
7.27_654.56	15177	13797	15640	17040	16218	19248	20411	19659	16093	17150	16957	19657	19578	19818	17000	23591	27503	27073	29343	22428	26611	32626	28040	29044
7.12_655.56	7587	7574			8528	9902	11288	9752		8114	9062	10771	9106		7970	12124	13061		13270	11838	12343	15716	14720	15068
7.25_656.56	6447	6545			6736	8249	8587	8287	6527	6662	7495	8792	7965		6936	9690	11842		11914	9711	10612	13409	11711	12062
7.86_658.58	18637	19132	20784	4495	19380	4889	5334	22209	20572	3999	21364	21624	22824	23057	19872	23669	25948	25462	27626	23144	25486	4331	27643	28955
7.58_668.58	10230	10300	11386	11403	10399	12665	13490	12557	11189	10879	11801	13509	16480	16492	14402	17935	21117	20861	22797	18675	19149	24454	22020	22350
7.56_678.6	5768	5850	6912	6589	6205	7387	8191	7494	6676	5798	7293	8029	10431	10253	8875	9510	13513	11991	15466	10880	11691	15588	13916	13520
7.22_680.58	19790	20180	20995	24874	22802	24512	27596	25755	21440	20923	22997	25438	37718	35963	31776	37860	42805	42278	49466	43049	46677	54183	49320	49616
7.79_682.59	53405	51272	54876	57878	54799	61139	67277	66684	58409	52783	56888	61453	75089	79453	69777	76027	88753	84631	96357	86306	86764	104938	96266	96941
8.45_686.61	36190	36243	40114	37535	5778	40077	45163	42148	40416	35063	38413	40439	42227	42570	36380	36718	44744	44579	47585	2858	42985	51180	47060	46796
5.14_688.49	21698	21732	24831	23129	25143	24556	28232	25285	24975	21113	24412	24644	15340	15166	12443	8713	10694	10416	10434	9495	8792	11185	9840	10480
7.22_690.6	12673	12417	15324	15412	15018	17279	19489	17678	14894	14235	15637	17904	26586	26007	20911	23843	32201	29212	42319	29519	33441	42828	37610	38236
8.64_698.62	6074	6578	6949	7003	6889	6962	7902	7538	7470	6094	6690	7145	6336	6619	5727	5729	7973	6917	8748	6286	7337	9152	8120	8546
7.23_707.59	4135			4715	4342	4926	4998	5608		4732	4665	5279	6030	6272	5632	7384	6881	7089	7699	7262	7831	8442	8807	7937
4.86_712.49	15259	15114	18909	16775	18842	17181	21001	19541	17813	13643	16907	15765	10737	12126	8688		8358	7115	14446	8748	8159	9259	7979	8271
4.79_736.49	15411	14899	18237	16267	17418	17169	20709	18219	19803	16850	19390	18295	18515	18581	15394	14236	17855	16306	20844	16538	17362	19834	16906	19066
5.19_741.52	6558	6689	7092	6522	6701	6951	7772	7081	7457	6567	7245	7489	5630		5434	4648	5512	5117	5972	4917	5229	5223		5090
6.48_744.55	69511	66155	79754	73621	72786	72088	80143	74137	73303	62700	67973	65734	60065	65205	55195	47127	57634	52365	61160	50957	49809	53335	48859	50696
4.3_745.53	5409	5810	6456	5533	6055	5599	5813	5643	5124	5083	5358	5449	9229	9331	8145	5253	7116	6353	8556	6877	6499	8209	7224	7166
5.09_749.57	3667	3702	4113	3928	4246	3943	4886	4056	4101	3457	3669	3892	4777	5390	3846	3695	5012	4270	5300	4498	4320	4657	4469	4702
7.78_760.61	4337		4707	5056	4776	5173	5722	5083	4552	4351	4764	5307	5996	6009	5498	5555	7257	6532	8001	6456	7129	8761	8055	7471
5.05_765.52	6689	6474	6907	7271	6664	7016	7747	7221	6772		7038	6967	5627	6309	5370		5477	4833	6426	5599	5026	5236	5480	5371
5.86_767.54	286401	279701	323274	297279	308662	310178	332466	305212	6317	247116	281778	289334	277508	280086	243939	214197	270305	249297	274354	239333	237550	259819	232985	249488
6.14_768.55				37134	37746					30200	33252		47972		41144	33481				37730	38121	44307	38060	39782
6.14_769.56	16182	15639	19535			16741	20056	17766	17288			16348		22315			19588	18013	21754					
5.38_775.56	29604	28731	14185	32559	32523	33117	32876	31698	30769	27974	10846	30612	22792	22323	19595	13385	17090	16062	19914	14662	14409	19869	16917	17769
4.52_782.5		4469		5348			5114	4886	4797			4172	5067	5106		4712	5981	4988				5034	5201	4942
5.22_786.54	7454	8584		10588	10659	6824	9371	9255	9295	7828	8618	7263	17205	13175	12324	15206	19774	17560	16149	12889	11994	5903	15746	15707
5.13_788.52	43284	40171	46962	40189	39668	41171	52447	44119	45780	41310	42291	46086	56218	56748	49277	43111	55050	49387	69169	54874	55131	53524	46717	49060
5.65_788.56	10459	11200	13785	13040	11594	12506	10653	13071	10499	10023	11261	11198	17886	19318	15717	15441	23304	20064	18947	14880	17284	19353	17173	17148
5.68_790.54	176863	170334	192091	174394	182024	179652	198509	173022	179318	145967	162609	163979	167906	171789	146192	112410	146173	131743	182630	140055	143935	162092	140214	145623
5.31_790.55			9913	9954	9030	9724	10498		10001	8293		9078	7859	7740		5775				8165	7612			
5.89_792.55	29491	30351	34198	31124	31038	31027	35016	31494	31254	26810	29355	29000		20618	17115	14293	18417	17579		17638	19087		17662	18031
5.13_794.53	3560	3790	3746	3659	4044	4128	4186	4091	3902	3670	3930	3427	4046	4136	3642	3593	3720	3534	3984	3940	4009	4083	4041	3628
5.61_794.55	5261	4870	5118	5300	5333	4997	5150	5094	5296	4947	5126	5163	5072	4732	4801	4964	4761	4795	5055	5487	4770	5014	5038	4967
6.33_794.57	18267	17782	21880	19198	19297	18793	22041	19126	18994	16460	17955	17829	8671	9143	7377	6759	9055	8007	10880	8538	8617	8362	7995	7811
4.3_795.52			6680		5445		5949	5759	5004	4876	5147	5373	9622	9030	7992	6320	8113	7121	11520	7911	8526	9312	8725	8955
4.51_797.53													8312	7835	6731	4575	6184	5555	7663	6331	6054	7772	6453	6813
4.91_799.55				4535								4627	21649	22200	17342	9828	18370	14652	26649	16534	17551	21974	16269	19198
4.71_800.54	30371	30625	35317	31540	32274	33760	35721	32754	33913	27998	29203	29731	33060	34040	29079	20313	27963	24930	37052	29651	29603	32455	28689	30013
4.94_801.56		6392	8786		5969	6681	8853	6320				7273	12386	13117	10968	6791	9892	9109	14570	9223	10138	11941	8558	10580
4.38_806.5													21775	21761	19068	19068	22438	22347	20554	16531	17427	22500	21265	22024
5.13_810.53	59229	64143	77712	75825	72870	62350	66122	65952	60856	41746	51448	48574	41720	41651	34814	28003	39308	36605	40073	28494	33699	39777	34761	37968
5.38_812.56	7061	8163	9363	9000	8470	7907	8391	8557	7445	7431	8468	8201	12753	11549	12468	11927	13418	13400	12633	11316	11958	12797	12345	12822
3.84_818.55	4618	4409	5409	4722	4568	4405	5516	4502	4515	3273	4241	4057		19700	15211	12355	17565	15267	17861	14999	14851	16407	14165	17782
5.77_820.56	4378	4255	4184	4406	4165	3881	4659	4883	4379	3987	3854	4104	5054	4990	4521	4222	4347	4538	4543	5598	4613	4397	4462	4541
5.1_829.57	7177	7474	8068	7127	7906	7540	8467	7772	8308	33457	7375	7869	6855	5999	5525	5417	5788	32613	6738	6066	5870	36422	5505	5860
5.87_834.53		7357	6779	7211	7254	6560	6937	7069	6927	7385	6673	7273			6957	7781		7194	6648	7344				
5.12_837.55	6713	7478	9997	8292	7983	7562	8664	8058	7196	5834	6383		7175		5434		7086	6497	6450	4987	6157	7581	6265	7177
5.96_837.59	13835	12825	15257	12593	12997	13091	15329	14442	14171	11385	13036	13202												
5.48_838.58	3867	4354	5290	4963	4459	4560	4188	4573	3898	3945	3950	3754	4343	4508	4324	3565	4818	4407	5541	4882	4937	4947	4240	4638
5.63_855.59	5139	5091	5373	53486	53978	5467	58459	54821	5444	4800	4871	51677	42680	41678	37098	31771	38067	35996	45295	37846	38369	40300	36835	37951
7.77_857.69	2237	2291	2333	2575	2760	2243	2735	2402	2067	2343	2306	2410	4339	3435	3309	2927	3402	3299	3173	2723	2905	2805	3173	2788
3.72_865.5													15119	14339	10369	10756	12892	11811	14807	11670	12354	13167	10618	11660
5.43_888.57	5041	5924	5881	5790	5122	4601				4852	4650		5233	5280	4367	4220	5328				4757	5604	5452	5370
5.04_911.57	28155	29943	35193	30168	30638	25668	28701	30075	29106	22560	27304	23818	14342	13995	12719	11722	14755	12880	13267	10991	13343	16232	14935	14929
5.42_913.58	8719	9144	11036	10125	9311	8896	9859	10132	8672	8047	8772	8101					4185					4855		4609
1.44_955.97		6763	11149	2092	2473	7752	58683			2158	3124			2060		3250	3423	2261	2087	2294	3279	9048		2113
13.41_955.97	133298	13931	2350	2494	2008	3517	5111	3195		2700	47518	4589	2426	168436	2191	4373	3419	8404	9910	2280	15352	48822	50145	9126
2.16_955.97		21448	2980	2035	2099	2983	5400		13989	2275	2583					5240	2004	2598	2010		2421	2709		
12.48_966	18961	89561	25735	24378	20090	216018	669337	39422	17943	25589	108127	25469	21210	22541	20763	217629	31994	101526	49531	21867	76455	40027	66354	391124
13.19_966	884828	95155	16679	17100	14420	26209	42350	22398	12802	17511	183542	30963	14753	1100397	13123	42232	26487	51852	78850	14846	110329	35012	138756	88989
12.45_966	18961	89561	25735	24378	20090	216018	669337	39422	17943	25589	108127	25469	21210	22541	20763	217629	31994	101526	49531	21867	57331	40027	66354	391124
13.37_967	185312	18878	3732	3804	3353	5164	7698	4608	3126	4022	49960	6147	3336	241014	3103	6332	5333	10642	15449	3376	26283	66635	28232	13417
9.06_1421.95	6940	7678	9782	7287	7101	6641	7998	7596	7376	6052	7032	6448	5177	5005	4520		5389	5066		4274		5287		5037
9.47_1449.98	8030	8185	9909	8432	8265	7679	8784	8009	7401	6870	7285	7613	8332	8779	7730	8006	10100	8939	8584	7140	7865	9537	8282	8924
9.15_1469.94	6026	6474	7778	6891	6329	6362	7123	6564	6297	5747	6140	5849	7038	8232	6427	6538	7808	7690	6956	5775	6331	7309	7129	7563
5.21_1478.02	6677		7589	7355	7344	7112	9222	8301	8387	6785	7849	7935							4608					
5.83_1484.07	6888	6986	8240	7475	7609	7234	8100	7682	7357	6040	6760	6311		5289										
5.97_1486.08	6090		7839	6740	7139	6830	9543	7543	7318	5630	6629	6752	6171	7062	5351	4354	6286	5244	5856			6309		5500
5.17_1496.07	9153	9077	10410		9144	10125	11070	10826		8125	10090	10016	7957			5970			8431	7322	7444	7562		7428
5.78_1502.11	5418		6158	5459		5685	7180	5716	5672	4442	5254	5050		4105			3892							
5.63_1509.11	3487	3370	3292	3363	3492	3296	3552	3337	3099	3194	3848	3275	3462	3464	3573	3626	3756	3805	3449	3743	3568	3306	3595	3737
5.91_1510.08	6301	5876		6969	6502	6244	8401	7356	6564	5527	6648	6863	6280	6201	5153	4643		5329	6618	5013	4518	5779		
5.87_1534.08	31321		35740	32516	32905	33716	35703	33386	31574	25130	28597	31188	28702	28408	23978	20298	28951		28829	22676	22387	26569	22029	24528
5.04_1544.07	12121	12040				14017		12856	13060	11179			10012		8616	7198	9529	8450		8985	8384		8217	8652
5.65_1550.12	5690	6107		6054	6290	6533	6700	6136				5920	6206				5503	4909	6749			6084	5074	
5.81_1552.13		7959	9066		8421		9433		8769		7570	8393	8513	9557	7863		8544	7916	9949			9372	8133	8625
5.13_1560.12	29931	31477	34286	30659	32709	32799	35689	33347	31743	25299	27724	29421	36067	34929	30587	26604	33774	30434	40491	32903	32243	33050	30245	31343
5.13_1561.12	28787	28433	33742	30009	31416	30293	34515	30862	30722	26222	28334	28474	34297	34189	29533	26111	31014	29841	37820	31832	31646	32334	27421	31515
5.04_1608.12	6879	7300	7765	6938	7207	7939	8283	8419	7327	6397	6870	7545	5340	4975	4387	3917	4784	4247	6192	5108	4932	5231	4425	4670
5.04_1609.12	7127	6862	7806	7737	7388	7590	8130	7305	7928	6622	6895	8076	5208	5412	4345	3526	4591	4653	5791	5213	4676	5206	4347	4816
5.02_1668.11			6351	5746		5421	5922	5936	5197	4896		4840			5244	5094		5763		5100			6157	6243
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Quantified m/z	Retention Time	KEGG ID	PubChem ID
5.06_554.48	554.4787997	5.055230769
5.98_584.53	584.5256022	5.9764
5.73_572.48_5.74_582.51	572.4813325_582.5101624	5.728_5.735708333
5.15_580.49	580.4944229	5.1514375
6.44_610.54	610.5413259	6.441458333
7.17_628.54_7.14_638.57	628.5439948_638.5724386	7.170892857_7.143041667
7.5_652.59	652.5880826	7.496041667
7.82_656.58_7.79_666.6	656.5751365_666.6032907	7.821888889_7.786703704
7.24_664.59	664.5879122	7.236583333
8.14_670.59_8.13_680.62	670.5910758_680.6194225	8.143037037_8.130807692
8.45_694.64_8.45_684.61	694.6352449_684.6065753	8.450111111_8.453481481
7.77_692.62	692.6193757	7.770925926
9.15_1447.96	1447.963142	9.152916667
3.29_281.25	281.248898	3.291407407
2.9_279.23	279.2332039	2.895925926
2.8_303.23	303.2331895	2.799518519
7.76_856.69	856.6875884	7.762461538
1.58_540.33	540.3306469	1.584962963
1.25_564.33	564.3306745	1.2513125
1.62_452.28	452.2782281	1.6185
4.92_750.53	750.5312542	4.917296296
5.53_778.56	778.5602112	5.532925926
4.99_776.54	776.5444301	4.994740741
4.53_774.53	774.5285535	4.526111111
4.81_788.54	788.5434958	4.813423077
6.21_806.59	806.5936243	6.210148148
5.61_804.58	804.5758	5.608888889
5.13_802.56_5.13_870.55	802.5604926_870.547649	5.132851852_5.132555556
5.42_816.58	816.5753106	5.420740741
6.28_832.61	832.6072478	6.281
5.77_830.59_5.77_898.58	830.5914883_898.5785173	5.774407407_5.770296296
5.3_828.58	828.5750755	5.297481481
5.04_826.56	826.5602507	5.04137037
4.64_824.54	824.5444945	4.642814815
5.95_856.61	856.6066635	5.953740741
5.69_854.59	854.5916692	5.685925926
5.08_852.58	852.5755751	5.075666667
4.86_850.56	850.5601761	4.860518519
6.14_882.62	882.6225979	6.14075
5.69_880.61	880.6064262	5.691518519
5.5_878.59	878.5913448	5.498814815
5.78_716.52	716.523454	5.784625
5.31_714.51	714.5079091	5.309375
5.14_742.54	742.5381243	5.136222222
5.97_742.54	742.5391744	5.966703704
5.38_740.52	740.5225782	5.38372
5.19_738.51	738.5080687	5.190407407
5.87_766.54	766.5391111	5.865
5.07_762.51	762.513546	5.065241379
5.37_840.58	840.5751081	5.367518519
5.26_764.52	764.5231133	5.255125
5.1_747.52	747.5184555	5.1
4.8_773.54	773.5354239	4.801555556
4.37_771.52	771.5180715	4.366708333
4.01_769.5	769.502224	4.014958333
4.19_819.52	819.5178108	4.186333333
3.86_817.5	817.5024096	3.855416667
4.39_807.5	807.5019455	4.386166667
4.93_835.53	835.5333633	4.933541667
4.51_833.52	833.5182594	4.508111111
5.53_863.56	863.5647662	5.534307692
5.09_861.55	861.5495459	5.085333333
4.61_859.53	859.5332197	4.606708333
4.44_857.52	857.5180357	4.437703704
5.26_887.56	887.5647765	5.256148148
5.01_885.55	885.5499087	5.013111111
4.48_883.53	883.5332921	4.484416667
4.86_909.55	909.5492121	4.859666667
4.31_881.52	881.5180622	4.310909091
6.01_790.59	790.591236	6.01262963
7.13_818.63	818.6275686	7.133884615
6.14_700.53	700.5282157	6.139857143
6.27_726.54	726.5445679	6.268481481
5.49_722.51	722.5127202	5.49362963
5.52_748.53	748.5285023	5.522555556
5.31_746.51	746.5127603	5.31437037
6.65_778.58	778.5750829	6.648315789
6.19_776.56	776.5598539	6.187583333
5.98_774.54	774.5437055	5.977
5.59_872.56	872.5636233	5.593481481
5.13_792.53	792.5322754	5.131407407
4.23_719.53	719.533801	4.229259259
4.52_733.55	733.5495494	4.521703704
4.82_747.57	747.5653381	4.820740741
4.82_815.55	815.5534828	4.816333333
5.49_775.6	775.596431	5.493481481
6.19_803.63	803.627481	6.194481481
6.88_831.66	831.658983	6.875259259
6.28_829.64	829.643247	6.282259259
7.21_845.67	845.6739457	7.208666667
6.58_843.66	843.6578311	6.581
7.54_859.69	859.6904919	7.543740741
6.84_857.67	857.6748601	6.840148148
6.28_855.66	855.6588259	6.276407407
5.77_1616.18	1616.182587	5.772925926
6.54_817.64	817.6426879	6.542
8.12_697.61	697.6098306	8.122769231
8.45_711.63	711.6253651	8.446481481
2.36_568.36	568.3619172	2.357703704
4.94_325.18	325.1843653	4.936111111
5.61_1564.15	1564.151393	5.60652381
5.77_1617.19	1617.186173	5.770962963
7.77_709.61	709.6095959	7.76662963
5.98_810.53	810.5266796	5.976857143
12.14_119.04	119.0363281	12.14283333
13.76_119.04	119.036331	13.763
0.52_151.03	151.0265045	0.522428571
4.46_160.84	160.8422305	4.457962963
5.4_162.84	162.8389491	5.40462963
4.52_162.84	162.8393291	4.516962963
0.51_179.06	179.0559908	0.5128
9.14_197.81	197.8082028	9.142714286
11.36_230.05	230.0492312	11.36327778
10.65_230.05	230.0492725	10.64722222
4.97_231.93	231.9318372	4.965461538
4.93_239.1	239.0963306	4.932192308
0.73_307.1	307.0979983	0.73025
4.93_311.17	311.1690315	4.934592593
1.56_313.14	313.1432437	1.560851852
1.57_323.17	323.1716075	1.567037037
2.66_327.23	327.2333247	2.660208333
4.93_367.24	367.2441431	4.929296296
4.38_446.38	446.3764307	4.377148148
3.18_465.3	465.3044812	3.183962963
4.91_479.35	479.3518282	4.914851852
2.41_480.31	480.3095449	2.414041667
3.19_505.34	505.335213	3.192185185
4.93_505.35	505.3510568	4.927555556
5.39_558.47	558.4655119	5.393789474
5.96_581.53	581.5259375	5.957962963
6.48_600.51	600.5134529	6.478137931
6.68_602.53	602.5279018	6.68
3.73_605.41	605.4055313	3.73155
5.85_608.53	608.5256096	5.848521739
6.44_627.53	627.530769	6.438571429
7.49_642.56	642.5595627	7.490541667
7.27_654.56	654.5604588	7.267392857
7.12_655.56	655.5624374	7.124052632
7.25_656.56	656.5600082	7.24585
7.86_658.58	658.5824246	7.863461538
7.58_668.58	668.5751991	7.578541667
7.56_678.6	678.6037025	7.562375
7.22_680.58	680.5751204	7.223269231
7.79_682.59	682.5909966	7.785074074
8.45_686.61	686.6119121	8.454461538
5.14_688.49	688.4920858	5.143208333
7.22_690.6	690.6035652	7.216208333
8.64_698.62	698.622935	8.638125
7.23_707.59	707.5931934	7.227809524
4.86_712.49	712.4919553	4.85773913
4.79_736.49	736.4920896	4.787708333
5.19_741.52	741.5170635	5.191454545
6.48_744.55	744.5545247	6.479458333
4.3_745.53	745.5338051	4.304148148
5.09_749.57	749.571829	5.092296296
7.78_760.61	760.6090473	7.777347826
5.05_765.52	765.517506	5.045136364
5.86_767.54	767.5427128	5.856888889
6.14_768.55	768.5542018	6.138
6.14_769.56	769.5578562	6.1425
5.38_775.56	775.5601036	5.377925926
4.52_782.5	782.4977395	4.515142857
5.22_786.54	786.5443567	5.223307692
5.13_788.52	788.5246544	5.128625
5.65_788.56	788.5556556	5.653074074
5.68_790.54	790.5398572	5.682458333
5.31_790.55	790.552286	5.309076923
5.89_792.55	792.5547587	5.891571429
5.13_794.53	794.5319557	5.134518519
5.61_794.55	794.5499076	5.612555556
6.33_794.57	794.5700048	6.328083333
4.3_795.52	795.5179245	4.2996
4.51_797.53	797.5332921	4.51175
4.91_799.55	799.5486908	4.911214286
4.71_800.54	800.5448193	4.705222222
4.94_801.56	801.5625626	4.935684211
4.38_806.5	806.4975662	4.379583333
5.13_810.53	810.5336496	5.125296296
5.38_812.56	812.5560404	5.375555556
3.84_818.55	818.5466006	3.836076923
5.77_820.56	820.5624162	5.767666667
5.1_829.57	829.5712355	5.096
5.87_834.53	834.5269413	5.874875
5.12_837.55	837.5476452	5.124095238
5.96_837.59	837.588061	5.963416667
5.48_838.58	838.5808226	5.479333333
5.63_855.59	855.5945161	5.629703704
7.77_857.69	857.6900526	7.768807692
3.72_865.5	865.5020752	3.721833333
5.43_888.57	888.5683989	5.426823529
5.04_911.57	911.5650508	5.040208333
5.42_913.58	913.58054	5.420466667
1.44_955.97	955.9716613	1.44226087
13.41_955.97	955.971681	13.40596429
2.16_955.97	955.971723	2.162947368
12.48_966	966.000733	12.47783333
13.19_966	966.000733	13.1944
12.45_966	966.0007331	12.45046667
13.37_967	967.0036862	13.36633333
9.06_1421.95	1421.947397	9.06235
9.47_1449.98	1449.978343	9.471083333
9.15_1469.94	1469.944743	9.153208333
5.21_1478.02	1478.020981	5.2095
5.83_1484.07	1484.068373	5.827923077
5.97_1486.08	1486.083774	5.96935
5.17_1496.07	1496.068201	5.173764706
5.78_1502.11	1502.114334	5.780833333
5.63_1509.11	1509.109474	5.634962963
5.91_1510.08	1510.082666	5.91265
5.87_1534.08	1534.083593	5.866272727
5.04_1544.07	1544.067952	5.0392
5.65_1550.12	1550.11554	5.648428571
5.81_1552.13	1552.130276	5.81325
5.13_1560.12	1560.120316	5.133518519
5.13_1561.12	1561.123694	5.132777778
5.04_1608.12	1608.120164	5.040703704
5.04_1609.12	1609.123132	5.042592593
5.02_1668.11	1668.112544	5.016142857
METABOLITES_END
#END