#METABOLOMICS WORKBENCH cordeirofb_20170406_154513_mwtab.txt DATATRACK_ID:882 STUDY_ID:ST000585 ANALYSIS_ID:AN000900 PROJECT_ID:PR000428
VERSION             	1
CREATED_ON             	April 11, 2017, 12:58 pm
#PROJECT
PR:PROJECT_TITLE                 	Follicular fluid Metabolomics
PR:PROJECT_TYPE                  	MS qualitative analysis
PR:PROJECT_SUMMARY               	Metabolomics profiling of follicular fluid samples from patients undergoing in
PR:PROJECT_SUMMARY               	vitro fertilization treatment
PR:INSTITUTE                     	SAO PAULO FEDERAL UNIVERSITY
PR:DEPARTMENT                    	DEPARTMENT OF SURGERY, DIVISIONOF UROLOGY, HUMAN REPRODUCTION SECTION
PR:LABORATORY                    	CENTER OF RESEARCH IN UROLOGY
PR:LAST_NAME                     	CORDEIRO
PR:FIRST_NAME                    	FERNANDA
PR:ADDRESS                       	Rua Embau, 231, Vila Clementino
PR:EMAIL                         	fernandabertuccez85@gmail.com
PR:PHONE                         	11996667402
#STUDY
ST:STUDY_TITLE                   	METABOLOMIC PROFILING OF FOLLICULAR FLUID FROM PATIENTS WITH INFERTILITY-RELATED
ST:STUDY_TITLE                   	DEEP ENDOMETRIOSIS.
ST:STUDY_SUMMARY                 	the metaboloc qualitative profiling was performed by LC-MS in follicular fluid
ST:STUDY_SUMMARY                 	samples of controls and endometriosis patients undergoing in vitro fertilization
ST:STUDY_SUMMARY                 	treatment
ST:INSTITUTE                     	Sao Paulo Federal University
ST:LAST_NAME                     	Cordeiro
ST:FIRST_NAME                    	Fernanda
ST:ADDRESS                       	Rua Embau, 231, Vila Clementino
ST:EMAIL                         	fernandabertuccez85@gmail.com
ST:PHONE                         	11996667402
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	40
ST:NUM_FEMALES                   	40
#SUBJECT
SU:SUBJECT_TYPE                  	Human Follicular Fluid
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	18-40
SU:GENDER                        	female
SU:HUMAN_MEDICATIONS             	Controled ovarian Stimulation
SU:HUMAN_EXCLUSION_CRITERIA      	Polycystic ovary syndrome, cancer, premature ovarian failure.
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_1	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_2	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_3	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C01_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C01_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C02_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C02_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C03_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C03_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C04_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C04_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C05_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C05_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_4	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C06_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C06_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C07_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C07_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C08_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C08_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C09_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C09_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C10_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C10_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_5	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C11_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C11_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C12_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C12_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C13_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C13_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C14_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C14_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C15_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C15_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_Control_6	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C16_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C16_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C17_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C17_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C18_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C18_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C19_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C19_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C20_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C20_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C21_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C21_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C22_1	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	C22_2	Sample Type:Control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_1	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_2	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_3	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END01_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END01_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END02_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END02_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END03_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END03_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END04_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END04_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END05_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END05_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_4	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END06_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END06_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END07_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END07_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END08_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END08_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END09_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END09_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END10_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END10_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_5	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END11_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END11_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END12_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END12_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END13_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END13_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END14_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END14_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END15_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END15_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	QC_END_6	Sample Type:Quality control	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END16_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END16_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END17_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END17_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END18_1	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
SUBJECT_SAMPLE_FACTORS           	-	END18_2	Sample Type:Endometriosis patient	Sample Preparation=Bligh & Dyer (1959); Amount of sample=50 µL; Type of extract collected=polar and apolar phases; Amount for LC-MS=5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v); Time of run=17 minutes
#COLLECTION
CO:COLLECTION_SUMMARY            	The FF samples were collected based on follicular aspiration after ovarian
CO:COLLECTION_SUMMARY            	controlled stimulation protocol, defined by the doctor prior to the begining of
CO:COLLECTION_SUMMARY            	the treatment. Follicular fluid samples were collectd and centrifuged at 800 ×
CO:COLLECTION_SUMMARY            	g for 10 minutes to separate the fluid from follicular cells and the samples
CO:COLLECTION_SUMMARY            	were maintained frozen in -20 °C until the metabolites extraction.
#TREATMENT
TR:TREATMENT_SUMMARY             	Does not apply. Patients were treated for infertility according to their medical
TR:TREATMENT_SUMMARY             	needs. The present study has no interference on patients management and it is
TR:TREATMENT_SUMMARY             	defined as a prospective case-control study.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The FF samples were centrifuged at 800 × g for 10 minutes to separate the fluid
SP:SAMPLEPREP_SUMMARY            	from follicular cells and the samples were maintained frozen in -20 °C until
SP:SAMPLEPREP_SUMMARY            	the metabolites extraction. The Bligh & Dyer protocol (1959) was applied for
SP:SAMPLEPREP_SUMMARY            	proteins removal and was performed as herein: 50 µL of sample was placed in a
SP:SAMPLEPREP_SUMMARY            	microtube, followed by addition of 50 µL of milli-Q water, 125 µL of
SP:SAMPLEPREP_SUMMARY            	chloroform (CHCl3 - Merck Millipore, MA, EUA) and 250 µL of methanol (MeOH -
SP:SAMPLEPREP_SUMMARY            	Merck Millipore - Massachusetts, EUA). The mixture was vortexed for 30 seconds.
SP:SAMPLEPREP_SUMMARY            	Next, polar and apolar phases were separated by the addition of 100 µL of
SP:SAMPLEPREP_SUMMARY            	milli-Q water and 125 µL of CHCl3 (Merck Millipore, MA EUA). Both polar and
SP:SAMPLEPREP_SUMMARY            	apolar phases were collected and transferred to a new microtube. The final
SP:SAMPLEPREP_SUMMARY            	content was dried by using a speedvac (CentriVap Cold Trap, Labconco, MO, USA).
SP:SAMPLEPREP_SUMMARY            	Prior to the LC-MS analysis, the metabolites were recovered by using 100 µL of
SP:SAMPLEPREP_SUMMARY            	acetonitrile and water (ACN:H2O 1:1, v:v - Merk Millipore, MA, USA) and filtered
SP:SAMPLEPREP_SUMMARY            	in 0.22 μm PVDF filters (Merck Millipore, MA, EUA).
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Ultima Q-TOF
CH:COLUMN_NAME                   	Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
CH:COLUMN_TEMPERATURE            	35 °C
CH:SOLVENT_A                     	H2O with formic acid 0.1%
CH:SOLVENT_B                     	Acetonitrile with formic acid 0.1%
CH:ANALYTICAL_TIME               	17 minutes
CH:CAPILLARY_VOLTAGE             	3 kV
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Waters Ultima QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST000585_AN000900_Results.txt
#END