#METABOLOMICS WORKBENCH cordeirofb_20170530_073740 DATATRACK_ID:928 STUDY_ID:ST000618 ANALYSIS_ID:AN000948 PROJECT_ID:PR000428 VERSION 1 CREATED_ON June 7, 2017, 1:11 pm #PROJECT PR:PROJECT_TITLE Follicular fluid Metabolomics PR:PROJECT_TYPE MS qualitative analysis PR:PROJECT_SUMMARY Metabolomics profiling of follicular fluid samples from patients undergoing in PR:PROJECT_SUMMARY vitro fertilization treatment PR:INSTITUTE Sao Paulo Federal University PR:DEPARTMENT Department of Surgery, Division of Urology, Human Reproduction Section PR:LABORATORY Center of research in Urology PR:LAST_NAME Bertuccez Cordeiro PR:FIRST_NAME Fernanda PR:ADDRESS Rua Embau, 231, Vila Clementino, Sao Paulo, São Paulo, 04039-060, Brazil PR:EMAIL fernandabertuccez85@gmail.com PR:PHONE 5511996667402 PR:FUNDING_SOURCE National Council for Scientific and Technological Development (CNPq - Brazil) #STUDY ST:STUDY_TITLE Metabolomic Profiling of Follicular Fluid from Patients with Polycystic Ovary ST:STUDY_TITLE Syndrome and Hyper Response to In Vitro Fertilization Treatment ST:STUDY_TYPE MS quallitative analysis ST:STUDY_SUMMARY The present study consisted in a metabolomic approach in follicular fluid ST:STUDY_SUMMARY samples from patients with polycystic ovary syndrome and hyper response to in ST:STUDY_SUMMARY vitro fertilization treatment. ST:INSTITUTE Sao Paulo Federal University ST:DEPARTMENT Department of Surgery, Division of Urology, Human Reproduction Section ST:LABORATORY Center of research in Urology ST:LAST_NAME Cordeiro ST:FIRST_NAME Fernanda ST:ADDRESS Rua Embau, 231, Vila Clementino ST:EMAIL fernandabertuccez85@gmail.com ST:PHONE 11996667402 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 81 ST:NUM_FEMALES 81 #SUBJECT SU:SUBJECT_TYPE Human Follicular Fluid SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER female SU:HUMAN_EXCLUSION_CRITERIA cancer, endometriosis, premature ovarian failure #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - QC_Control_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_Control_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_Control_3 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C01_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C01_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C02_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C02_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C03_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C03_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C04_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C04_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C05_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C05_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_Control_4 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C06_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C06_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C07_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C07_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C08_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C08_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C09_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C09_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C10_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C10_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_Control_5 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C11_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C11_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C12_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C12_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C13_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C13_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C14_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C14_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C15_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C15_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_Control_6 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C16_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C16_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C17_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C17_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C18_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C18_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C19_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C19_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C20_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C20_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C21_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C21_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C22_1 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - C22_2 Condition:Control Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_3 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR01_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR01_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR02_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR02_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR03_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR03_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR04_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR04_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR05_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR05_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_4 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR06_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR06_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR07_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR07_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR08_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR08_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR09_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR09_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR10_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR10_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_5 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR11_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR11_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR12_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR12_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR13_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR13_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR14_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR14_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR15_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR15_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_6 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR16_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR16_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR17_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR17_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR18_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR18_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR19_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR19_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR20_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR20_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_7 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR21_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR21_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR22_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR22_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR23_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR23_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR24_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR24_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR25_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR25_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_8 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR26_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR26_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR27_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR27_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR28_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR28_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR29_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR29_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR30_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR30_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_9 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR31_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR31_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR32_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR32_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR33_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR33_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR34_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR34_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR35_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR35_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_10 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR36_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR36_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR37_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR37_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR38_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR38_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR39_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR39_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR40_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR40_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_11 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR41_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR41_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR42_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR42_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR43_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR43_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR44_1 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - HR44_2 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_HR_12 Condition:Hyper Responder Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_3 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP01_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP01_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP02_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP02_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP03_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP03_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP04_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP04_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP05_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP05_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_4 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP06_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP06_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP07_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP07_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP08_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP08_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP09_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP09_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP10_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP10_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_5 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP11_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP11_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP12_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP12_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP13_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP13_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP14_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP14_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP15_1 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - SOP15_2 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases SUBJECT_SAMPLE_FACTORS - QC_SOP_6 Condition:Polycystic Ovary Syndrome Type of extract collected=polar and apolar phases #COLLECTION CO:COLLECTION_SUMMARY The patients were initially undergoing to controlled ovarian stimulation, from CO:COLLECTION_SUMMARY which follicular fluid samples were collected. #TREATMENT TR:TREATMENT_SUMMARY Follicular fluid samples were centrifuged at 800 × g for 10 minutes to separate TR:TREATMENT_SUMMARY the fluid from follicular cells and the samples were maintained frozen in -20 TR:TREATMENT_SUMMARY °C until the metabolites extraction. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 50 µL of follicular fluid samples were submitted to Bligh & Dyer protocol. SP:EXTRACTION_METHOD BLIGH & DYER SP:SAMPLE_RESUSPENSION 5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v) #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Liquid Chromatography was performed by using a chromatographer Acquity with a CH:CHROMATOGRAPHY_SUMMARY reverse phase column (Acquity UPCL HSS 1,8 µm, 2.1x100 mm - Waters Corporation, CH:CHROMATOGRAPHY_SUMMARY MA, USA), at 35 °C. The solvents for the elution were: A- H2O with formic acid CH:CHROMATOGRAPHY_SUMMARY 0.1% and B- ACN with formic acid 0.1%. The initial gradient was composed of 80% CH:CHROMATOGRAPHY_SUMMARY of A and 20% of B, during 1 minute. Next, the gradient was linearly changed for CH:CHROMATOGRAPHY_SUMMARY 50% of A and 50% of B, during the next minute. In the sequence, the gradient was CH:CHROMATOGRAPHY_SUMMARY changed for 35% of A and 65% of B during 2 minutes. The gradient was then CH:CHROMATOGRAPHY_SUMMARY inverted, considering 20% of A and 80% of B, during 3 minutes. For the next 10 CH:CHROMATOGRAPHY_SUMMARY minutes, the gradient was 3% of A and 97% of B. The initial condition was CH:CHROMATOGRAPHY_SUMMARY reestablished for the column washing during 1 minute. The total run time was 17 CH:CHROMATOGRAPHY_SUMMARY minutes and the solvents flux was 0.5 mL/min and the total amount of sample used CH:CHROMATOGRAPHY_SUMMARY per run was 5 µL. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Ultima Quadrupole-Time of Flight - Waters Corporation CH:COLUMN_NAME Waters Acquity HSS T3 (100 x 2.1mm, 1.8um) CH:FLOW_RATE 0.5 mL/min CH:COLUMN_TEMPERATURE 35 °C CH:SOLVENT_A H2O with formic acid 0.1% CH:SOLVENT_B ACN with formic acid 0.1% #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Waters Ultima QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST000618_AN000948_Results.txt UNITS:mass intensity #END