#METABOLOMICS WORKBENCH araskind_20170619_132403 DATATRACK_ID:1016 STUDY_ID:ST000717 ANALYSIS_ID:AN001121 PROJECT_ID:PR000514
VERSION             	1
CREATED_ON             	July 2, 2017, 1:17 pm
#PROJECT
PR:PROJECT_TITLE                 	Effect of aging on ENS microenvironment
PR:PROJECT_TYPE                  	MS analysis
PR:PROJECT_SUMMARY               	Assessing influence of microbiota on ENS with age
PR:INSTITUTE                     	Stanford University
PR:DEPARTMENT                    	Gastroenterology and Hepatology
PR:LABORATORY                    	Habtezion Lab
PR:LAST_NAME                     	Habtezion
PR:FIRST_NAME                    	Aida
PR:ADDRESS                       	Stanford, CA
PR:EMAIL                         	aida.habtezion@stanford.edu
PR:PHONE                         	650-736-5555
#STUDY
ST:STUDY_TITLE                   	Assessing influence of microbiota on enteric nervous system (ENS) with age
ST:STUDY_TYPE                    	MS analysis
ST:STUDY_SUMMARY                 	Freshly collected stool samples were immediately frozen in dry ice and stored at
ST:STUDY_SUMMARY                 	-80deg.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	1
#SUBJECT
SU:SUBJECT_TYPE                  	Mouse
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023725	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023726	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023727	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023728	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023729	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023730	Age:young stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023731	Age:old stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023732	Age:old stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023733	Age:old stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023734	Age:old stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023735	Age:old stool	
SUBJECT_SAMPLE_FACTORS           	SU0015385	S00023736	Age:old stool	
#COLLECTION
CO:COLLECTION_SUMMARY             	-
#TREATMENT
TR:TREATMENT_SUMMARY              	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY             	-
SP:SAMPLEPREP_PROTOCOL_FILENAME   	A007 - Short Chain Fatty Acids.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 7890N
CH:COLUMN_NAME                   	Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	A007 - Short Chain Fatty Acids.pdf
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 5975
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nmol/mg
MS_METABOLITE_DATA_START
Samples	S00023725	S00023726	S00023727	S00023728	S00023729	S00023730	S00023731	S00023732	S00023733	S00023734	S00023735	S00023736
Factors	Age:young stool	Age:young stool	Age:young stool	Age:young stool	Age:young stool	Age:young stool	Age:old stool	Age:old stool	Age:old stool	Age:old stool	Age:old stool	Age:old stool
PROPIONIC ACID	3.337806192	1.953088897	1.546781479	2.127732721	2.191130681	2.705228522	1.498801009	1.527559739	1.185419256	1.434447063	2.320918575	4.275983491
ACETIC ACID	14.98266682	17.93174519	13.53871913	15.54364482	25.68091522	21.0355069	10.99272627	16.78504486	9.97700172	8.979759058	18.88884949	14.11550993
BUTYRIC ACID	4.413211233	5.136151343	3.73925281	5.040021617	3.598735721	5.067286641	4.301809575	5.04395886	3.794715619	3.465979501	4.263460274	8.418439874
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
PROPIONIC ACID				1032
ACETIC ACID				176
BUTYRIC ACID				264
METABOLITES_END
#END