#METABOLOMICS WORKBENCH xujiuliang_20170704_000218 DATATRACK_ID:1109 STUDY_ID:ST000766 ANALYSIS_ID:AN001212 PROJECT_ID:PR000552
VERSION             	1
CREATED_ON             	July 5, 2017, 9:57 am
#PROJECT
PR:PROJECT_TITLE                 	Soybean project
PR:PROJECT_SUMMARY               	Non-targeted metabolic profiling of 26 soybean varieties using liquid
PR:PROJECT_SUMMARY               	chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS)
PR:PROJECT_SUMMARY               	and 11 cultivated black soybeans (CBS), combined with multivariate analysis,
PR:PROJECT_SUMMARY               	revealed significant differences in25 differential metabolites
PR:INSTITUTE                     	KIST
PR:LAST_NAME                     	Xu
PR:FIRST_NAME                    	Jiuliang
PR:ADDRESS                       	saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South
PR:EMAIL                         	213510@kist.re.kr
PR:PHONE                         	01024398802
#STUDY
ST:STUDY_TITLE                   	Differences in metabolite profiles and antioxidant activities between wild and
ST:STUDY_TITLE                   	cultivated black soybeans evaluated by a correlation analysis
ST:STUDY_SUMMARY                 	Non-targeted metabolic profiling of 26 soybean varieties using liquid
ST:STUDY_SUMMARY                 	chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS)
ST:STUDY_SUMMARY                 	and 11 cultivated black soybeans (CBS), combined with multivariate analysis,
ST:STUDY_SUMMARY                 	revealed significant differences in 25 differential metabolites
ST:INSTITUTE                     	KIST
ST:LAST_NAME                     	Xu
ST:FIRST_NAME                    	Jiuliang
ST:ADDRESS                       	saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South
ST:EMAIL                         	213510@kist.re.kr
ST:PHONE                         	01024398802
#SUBJECT
SU:SUBJECT_TYPE                  	plant
SU:SUBJECT_SPECIES               	Glycine max
SU:TAXONOMY_ID                   	3847
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	01_CBS_1	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	02_CBS_2	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	03_CBS_3	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	04_CBS_4	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	05_CBS_5	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	06_CBS_6	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	07_CBS_7	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	11_CBS_8	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	12_CBS_9	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	13_CBS_10	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	14_CBS_11	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	08_CBS_QC1	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	18_CBS_QC2	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	28_CBS_QC3	type:Cultivar	
SUBJECT_SAMPLE_FACTORS           	-	15_WBS_1	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	16_WBS_2	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	17_WBS_3	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	21_WBS_4	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	22_WBS_5	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	23_WBS_6	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	24_WBS_7	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	25_WBS_8	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	26_WBS_9	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	27_WBS_10	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	31_WBS_11	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	32_WBS_12	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	33_WBS_13	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	34_WBS_14	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	35_WBS_15	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	09_WBS_QC1	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	19_WBS_QC2	type:Wild	
SUBJECT_SAMPLE_FACTORS           	-	29_WBS_QC3	type:Wild	
#COLLECTION
CO:COLLECTION_SUMMARY            	Fifteen wild and 11 cultivated black soybeans were selected from the soybean
CO:COLLECTION_SUMMARY            	germplasm collections that have been maintained by the National Institute of
CO:COLLECTION_SUMMARY            	Crop Science in Korea
#TREATMENT
TR:TREATMENT_SUMMARY             	The planting density was 15 cm between plants in a row, and the rows were 70 cm
TR:TREATMENT_SUMMARY             	apart. Field management, including irrigation, fertilizer application, and pest
TR:TREATMENT_SUMMARY             	control followed essentially normal agricultural practices according to Rural
TR:TREATMENT_SUMMARY             	Development Administration standard protocol
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Dried black soybeans were ground into a fine powder and sieved through a screen
SP:SAMPLEPREP_SUMMARY            	with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous
SP:SAMPLEPREP_SUMMARY            	methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard
SP:SAMPLEPREP_SUMMARY            	and sonicated for 30 min. The mixture was kept for approximately 16 h at room
SP:SAMPLEPREP_SUMMARY            	temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm
SP:SAMPLEPREP_SUMMARY            	syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous
SP:SAMPLEPREP_SUMMARY            	methanol for LC-MS analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290
CH:COLUMN_NAME                   	Prevail C18
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Bruker Daltonics
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST000766_AN001212_Results.txt	UNITS:peal area
#END