#METABOLOMICS WORKBENCH beyondcpu_20170521_230456 DATATRACK_ID:921 STUDY_ID:ST000782 ANALYSIS_ID:AN001238 PROJECT_ID:PR000568 VERSION 1 CREATED_ON June 9, 2017, 5:07 am #PROJECT PR:PROJECT_TITLE Camellia nitidissima prevent colorectal cancer PR:PROJECT_TYPE Nuclear magnetic resonance-based metabolomics PR:PROJECT_SUMMARY Colorectal cancer (CRC) is one of the most common malignant tumors worldwide, PR:PROJECT_SUMMARY occurring in the colon or rectum portion of large intestine. With marked PR:PROJECT_SUMMARY antioxidant, anti-inflammation and anti-tumor activities, Camellia nitidissima PR:PROJECT_SUMMARY Chi has been used as an effective treatment of cancer. The azoxymethane/dextran PR:PROJECT_SUMMARY sodium sulfate (AOM/DSS) induced CRC mice model was established and the PR:PROJECT_SUMMARY prevention effect of Camellia nitidissima Chi extracts on the evolving of CRC PR:PROJECT_SUMMARY was evaluated by gross examination, histopathological inspection, serum PR:PROJECT_SUMMARY biochemistry analysis, combined with nuclear magnetic resonance (NMR)-based PR:PROJECT_SUMMARY metabolomics and correlation network analysis. The results showed that Camellia PR:PROJECT_SUMMARY nitidissima Chi extracts could significantly inhibit AOM/DSS induced CRC, PR:PROJECT_SUMMARY relieve the colonic pathology and ameliorate the serum biochemistry, and could PR:PROJECT_SUMMARY significantly reverse the disturbed metabolism towards the normal state. PR:PROJECT_SUMMARY Moreover, the butanol fraction showed a better efficiency than the water-soluble PR:PROJECT_SUMMARY fraction of Camellia nitidissima Chi. The study pave the way for further PR:PROJECT_SUMMARY development of Camellia nitidissima Chi extracts as a potent CRC inhibitor. PR:INSTITUTE Nanjing University of Science & Technology PR:DEPARTMENT Department of Biological Engineering PR:LABORATORY Center for Molecular Metabolism PR:LAST_NAME Li PR:FIRST_NAME Minghui PR:ADDRESS 200 Xiaolingwei Str., Nanjing, Jiangsu, 210094, China PR:EMAIL cpu_lmh@126.com PR:PHONE +8615952052370 #STUDY ST:STUDY_TITLE Nuclear magnetic resonance-based metabolomics approach to evaluate the ST:STUDY_TITLE prevention effect of Camellia nitidissima Chi on colitis-associated ST:STUDY_TITLE carcinogenesis ST:STUDY_TYPE Original Research ST:STUDY_SUMMARY Colorectal cancer (CRC) is one of the most common malignant tumors worldwide, ST:STUDY_SUMMARY occurring in the colon or rectum portion of large intestine. With marked ST:STUDY_SUMMARY antioxidant, anti-inflammation and anti- tumor activities, Camellia nitidissima ST:STUDY_SUMMARY Chi has been used as an effective treatment of cancer. The azoxymethane/dextran ST:STUDY_SUMMARY sodium sulfate (AOM/DSS) induced CRC mice model was established and the ST:STUDY_SUMMARY prevention effect of Camellia nitidissima Chi extracts on the evolving of CRC ST:STUDY_SUMMARY was evaluated by gross examination, histopathological inspection, serum ST:STUDY_SUMMARY biochemistry analysis, combined with nuclear magnetic resonance (NMR)-based ST:STUDY_SUMMARY metabolomics and correlation network analysis. The results showed that Camellia ST:STUDY_SUMMARY nitidissima Chi extracts could significantly inhibit AOM/DSS induced CRC, ST:STUDY_SUMMARY relieve the colonic pathology and ameliorate the serum biochemistry, and could ST:STUDY_SUMMARY significantly reverse the disturbed metabolism towards the normal state. ST:STUDY_SUMMARY Moreover, the butanol fraction showed a better efficiency than the water-soluble ST:STUDY_SUMMARY fraction of Camellia nitidissima Chi. The study pave the way for further ST:STUDY_SUMMARY development of Camellia nitidissima Chi extracts as a potent CRC inhibitor. ST:INSTITUTE Nanjing University of Science and Technology ST:LAST_NAME Li ST:FIRST_NAME Minghui ST:ADDRESS 200 Xiaolingwei Street, Nanjing 210094, China ST:EMAIL cpu_lmh@126.com ST:PHONE +86 15952052370 #SUBJECT SU:SUBJECT_TYPE Animals SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Intestine_Control1 Tissue:Intestine | Treatment:Control SUBJECT_SAMPLE_FACTORS - Intestine_Control2 Tissue:Intestine | Treatment:Control SUBJECT_SAMPLE_FACTORS - Intestine_Control3 Tissue:Intestine | Treatment:Control SUBJECT_SAMPLE_FACTORS - Intestine_Control4 Tissue:Intestine | Treatment:Control SUBJECT_SAMPLE_FACTORS - Intestine_Model1 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model2 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model3 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model4 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model5 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model6 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model7 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model8 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model9 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model10 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model11 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model12 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model13 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model14 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model15 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_Model16 Tissue:Intestine | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Intestine_JHC1 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC2 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC3 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC4 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC5 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC6 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC7 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC8 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC9 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC10 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC11 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_JHC12 Tissue:Intestine | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Intestine_CNC1 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC2 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC3 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC4 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC5 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC6 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC7 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC8 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Intestine_CNC9 Tissue:Intestine | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_Control1 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control2 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control3 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control4 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control5 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control6 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Control7 Tissue:Kidney | Treatment:Control SUBJECT_SAMPLE_FACTORS - Kideny_Model1 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model2 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model3 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model4 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model5 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model6 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model7 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model8 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model9 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model10 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model11 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model12 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model13 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_Model14 Tissue:Kidney | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Kideny_JHC1 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC2 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC3 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC4 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC5 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC6 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC7 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC8 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC9 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC10 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC11 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_JHC12 Tissue:Kidney | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Kideny_CNC1 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC2 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC3 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC4 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC5 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC6 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Kideny_CNC7 Tissue:Kidney | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_Control1 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control2 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control3 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control4 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control5 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control6 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control7 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control8 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control9 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Control10 Tissue:Spleen | Treatment:Control SUBJECT_SAMPLE_FACTORS - Spleen_Model1 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model2 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model3 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model4 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model5 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model6 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model7 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model8 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model9 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model10 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model11 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model12 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model13 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model14 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_Model15 Tissue:Spleen | Treatment:Carcinoma SUBJECT_SAMPLE_FACTORS - Spleen_JHC1 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC2 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC3 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC4 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC5 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC6 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC7 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC8 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC9 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC10 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_JHC11 Tissue:Spleen | Treatment:JHC SUBJECT_SAMPLE_FACTORS - Spleen_CNC1 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC2 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC3 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC4 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC5 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC6 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC7 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC8 Tissue:Spleen | Treatment:CNC SUBJECT_SAMPLE_FACTORS - Spleen_CNC9 Tissue:Spleen | Treatment:CNC #COLLECTION CO:COLLECTION_SUMMARY Briefly, tissues of intestines, kidneys and spleens weighting about 200 mg, 200 CO:COLLECTION_SUMMARY mg and 110 mg were weighted, homogenized with an icy cold solvent (the ratio of CO:COLLECTION_SUMMARY tissue weight to 50 % acetonitrile volume was 1: 5, w/v), vortexed, and CO:COLLECTION_SUMMARY centrifuged for 10 min at 12,000 g and 4 °C. The supernatant was transferred CO:COLLECTION_SUMMARY into fresh Eppendorf tube and was then frozen and lyophilized to dryness on a CO:COLLECTION_SUMMARY vacuum concentrator. The dried samples were stored at -80 °C until use. #TREATMENT TR:TREATMENT_SUMMARY The animals were maintained under controlled conditions (22°C, 12h/12h 98 TR:TREATMENT_SUMMARY dark/light cycle). At the 15th week, there were 15 surviving mice in control TR:TREATMENT_SUMMARY group, 17 in model group, 14 in JHC group and 10 in CNC group, respectively. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY For NMR measurements, samples were dissolved in 550 µL 99.8% deuteroxide (D2O) SP:SAMPLEPREP_SUMMARY phosphate buffer (0.2 M, pH = 7.4) containing 0.05% (w/v) sodium SP:SAMPLEPREP_SUMMARY 3-(trimethylsilyl) propionate-2, 2, 3, 3-d4 (TSP). After vortexing and SP:SAMPLEPREP_SUMMARY centrifugation, the supernatant was then transferred to a 5 mm NMR tube for 1H SP:SAMPLEPREP_SUMMARY NMR analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_NAME Bruker NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:SPECTROMETER_FREQUENCY 500 MHz NM:NMR_RESULTS_FILE ST000782_AN001238_Results.txt UNITS:NMR bins #END