#METABOLOMICS WORKBENCH araskind_20170714_081557 DATATRACK_ID:1133 STUDY_ID:ST000819 ANALYSIS_ID:AN001302 PROJECT_ID:PR000557 VERSION 1 CREATED_ON August 1, 2017, 3:02 pm #PROJECT PR:PROJECT_TITLE Changes in lipid levels with knock out of ACADM (shACADM) Lipidomics PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Looking for changes in lipid levels with knock out of ACADM PR:INSTITUTE University of Michigan PR:DEPARTMENT Molecular and Integrative Physiology PR:LABORATORY Lyssiotis Lab PR:LAST_NAME Lyssiotis PR:FIRST_NAME Costas PR:ADDRESS Ann Arbor, MI PR:EMAIL clyssiot@umich.edu PR:PHONE 734-615-9133 #STUDY ST:STUDY_TITLE Looking for changes in lipid levels with knock out of ACADM (shACADM) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM ST:STUDY_SUMMARY 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of ST:STUDY_SUMMARY medium chain fatty acids (C4-C12). A round of selection was performed using ST:STUDY_SUMMARY puromycin to ensure only cells that had been transduced survived. Knockdown of ST:STUDY_SUMMARY ACADM was confirmed via western blot. Pellets were collected by centrifuging ST:STUDY_SUMMARY cells for 10 min at max speed. The pellets were washed and each sample was split ST:STUDY_SUMMARY into quadruplicate. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 3 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0014234 S00021731 Transduced with:shACADM4 SUBJECT_SAMPLE_FACTORS SU0014234 S00021732 Transduced with:shACADM4 SUBJECT_SAMPLE_FACTORS SU0014234 S00021733 Transduced with:shACADM4 SUBJECT_SAMPLE_FACTORS SU0014234 S00021734 Transduced with:shACADM4 SUBJECT_SAMPLE_FACTORS SU0014235 S00021735 Transduced with:shACADM6 SUBJECT_SAMPLE_FACTORS SU0014235 S00021736 Transduced with:shACADM6 SUBJECT_SAMPLE_FACTORS SU0014235 S00021737 Transduced with:shACADM6 SUBJECT_SAMPLE_FACTORS SU0014235 S00021738 Transduced with:shACADM6 SUBJECT_SAMPLE_FACTORS SU0014236 S00021739 Transduced with:shACADMscr SUBJECT_SAMPLE_FACTORS SU0014236 S00021740 Transduced with:shACADMscr SUBJECT_SAMPLE_FACTORS SU0014236 S00021741 Transduced with:shACADMscr SUBJECT_SAMPLE_FACTORS SU0014236 S00021742 Transduced with:shACADMscr #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A004 - Shotgun Lipidomics.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Waters Xbridge BEH C18 (50 x 1mm, 3.5um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A008 - Acyl Carnitines.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/ug protein MS_METABOLITE_DATA_START Samples S00021731 S00021732 S00021733 S00021734 S00021735 S00021736 S00021737 S00021738 S00021739 S00021740 S00021741 S00021742 Factors Transduced with:shACADM4 Transduced with:shACADM4 Transduced with:shACADM4 Transduced with:shACADM4 Transduced with:shACADM6 Transduced with:shACADM6 Transduced with:shACADM6 Transduced with:shACADM6 Transduced with:shACADMscr Transduced with:shACADMscr Transduced with:shACADMscr Transduced with:shACADMscr ACETYL-DL-CARNITINE 15.20546067 18.07056182 11.24574751 11.99804636 12.28310183 14.26191246 13.37886282 14.17667391 14.88614288 13.55534447 15.24164069 16.65295116 DECANOYLCARNITINE .019825831 .023556327 .017296331 .015469811 .019865695 .023137137 .029889508 .027207317 .032477726 .026342887 .026964221 .033380675 LAUROYLCARNITINE .076009032 .079841127 .063232398 .057745946 .061003535 .077116223 .099731885 .115633126 .104729034 .07721505 .083590866 .110709395 L-PALMITOYLCARNITINE 1.652673647 2.116380703 1.558703454 1.343585015 1.264035074 2.118303482 1.96669206 2.044392507 2.950571025 2.220557337 2.681129048 3.544717052 O-OCTANOYLCARNITINE .029410786 .035269988 .025815132 .02362421 .026478902 .02914819 .035556273 .034548634 .034140647 .034516108 .029422466 .036698479 BUTYRYLCARNITINE 4.198895116 5.97455274 1.736132372 1.925018177 1.773224924 3.997991689 3.752709994 4.276347313 2.123717138 1.996315714 2.982466414 4.422392566 DL-CARNITINE 39.04638787 35.90193865 28.62596415 22.69878065 25.66590397 28.05625304 35.57130629 28.54777973 26.38395205 27.70516827 26.51128093 43.23183794 AC1MIY4G .223359713 .235742671 .152971439 .156842788 .149714063 .170986063 .173926083 .200902628 .17339216 .201690186 .130788128 .197626479 ARACHIDONYL CARNITINE .178152598 .285635074 .201952603 .165244515 .117392046 .257568796 .288207225 .231830006 .538386682 .297795456 .397421743 .547055516 ARACHIDYL CARNITINE .001262277 .001838382 .001392142 .000993326 .001924772 .00190596 .002735157 .001880514 .004201177 .00522658 .003439934 .004260206 LINOELAIDYL CARNITINE .284375381 .444721223 .315570023 .255897991 .268474417 .447359409 .475507873 .48480516 .708065132 .438848592 .552463604 .779834283 18:3 CARNITINE .122876233 .23643141 .14472722 .121376077 .148936081 .230763844 .270103852 .257197543 .461766132 .294976408 .379355094 .42267782 CIS-4-DECENOYL CARNITINE .000900918 .001158032 .000956743 .000955765 .000717847 .00098573 .001489242 .001174315 .001386959 .001404463 .001453604 .001499192 ISOVALERYLCARNITINE 2.652254125 2.937415265 2.117797375 2.004763588 1.860942903 2.417168977 2.502310528 2.49530889 2.170679638 2.264237426 1.980162821 2.505542768 MYRISTOYLCARNITINE .588138591 .780201822 .672898195 .490404598 .400745385 .578588376 .793121634 .823334224 1.29512326 .899480792 .998553208 1.33046501 STEAROYLCARNITINE .156742723 .237115312 .140397438 .154720961 .195263194 .273613444 .284990738 .288709887 .572525419 .346542636 .693505162 .639366341 OLEOYLCARNITINE .96764442 1.358441396 1.040343445 .936244733 1.174851185 1.941606779 2.037052701 2.004514605 2.73733414 2.033944686 2.534700612 3.115771646 GLUTARYLCARNITINE .030756535 .034013945 .023240727 .022134714 .023577981 .029096595 .027767981 .032645462 .02437852 .024295223 .023508632 .027784331 11-CIS-ICOSENEOYLCARNITINE .009066514 .015546614 .009366663 .00803587 .014136043 .017048793 .020769615 .022517112 .035595748 .02144162 .040072832 .038860346 (11Z,14Z)-ICOSADIENOYLCARNITINE .040319131 .065835285 .049416112 .043469882 .060185672 .094802412 .102017102 .099171672 .189938176 .112813376 .187150516 .227406054 3-HYDROXYLAUROYLCARNITINE .00638321 .006719879 .005990168 .004816496 .005306895 .006180265 .008422667 .0090673 .015488324 .008961206 .013576891 .016789917 3-HYDROXYMYRISTOYLCARNITINE .014853983 .017344924 .013999634 .010885017 .007930294 .008740426 .010724994 .013502193 .021963737 .016019515 .02138328 .025181908 3-HYDROXYPALMITOYLCARNITINE .031141264 .038381422 .034258577 .027028691 .032376225 .044669943 .04910566 .049214047 .066844467 .04832983 .056728447 .070087951 3-HYDROXYLINOLEYLCARNITINE .009090149 .011036852 .010030736 .008849648 .010700174 .018475314 .023838276 .020362177 .039894842 .018842113 .039443391 .048301783 16:1 CARNITINE .483084065 .688101889 .494762955 .419472318 .616721529 .944364128 1.002222948 1.019592279 1.83798215 1.278923267 1.600873438 1.940727011 8:1 CARNITINE .007483505 .006849324 .006784219 .00620255 .006308905 .007256629 .008469802 .008592152 .007122784 .006198747 .005889251 .007274132 14:2 CARNITINE .033464124 .043729134 .04364662 .032244228 .033680233 .043864069 .066290765 .068443206 .07928557 .052135792 .063624376 .070751383 12:1 CARNITINE .011001841 .013126047 .011665139 .009820332 .013256599 .015920587 .024833216 .02597028 .030529773 .019342797 .030556723 .032240898 14:1 CARNITINE .133452099 .192421467 .17530569 .141625931 .176187928 .259304473 .362029268 .335685018 .583522222 .345251589 .464835723 .482767334 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type ACETYL-DL-CARNITINE 1 DECANOYLCARNITINE 10245190 LAUROYLCARNITINE 10427569 L-PALMITOYLCARNITINE 11953816 O-OCTANOYLCARNITINE 123701 BUTYRYLCARNITINE 213144 DL-CARNITINE 288 AC1MIY4G 3082079 ARACHIDONYL CARNITINE 53477832 ARACHIDYL CARNITINE 53477833 LINOELAIDYL CARNITINE 53477834 18:3 CARNITINE 54500892 CIS-4-DECENOYL CARNITINE 57357170 ISOVALERYLCARNITINE 6426851 MYRISTOYLCARNITINE 6426854 STEAROYLCARNITINE 6426855 OLEOYLCARNITINE 6441392 GLUTARYLCARNITINE 71317118 11-CIS-ICOSENEOYLCARNITINE 71464507 (11Z,14Z)-ICOSADIENOYLCARNITINE 71464509 3-HYDROXYLAUROYLCARNITINE 71464535 3-HYDROXYMYRISTOYLCARNITINE 71464541 3-HYDROXYPALMITOYLCARNITINE 71464553 3-HYDROXYLINOLEYLCARNITINE 71464556 16:1 CARNITINE 76040365 8:1 CARNITINE 77908888 14:2 CARNITINE 87087940 12:1 CARNITINE 87087953 14:1 CARNITINE 87088002 METABOLITES_END #END