#METABOLOMICS WORKBENCH araskind_20170714_123806 DATATRACK_ID:1135 STUDY_ID:ST000820 ANALYSIS_ID:AN001303 PROJECT_ID:PR000584
VERSION             	1
CREATED_ON             	August 1, 2017, 3:36 pm
#PROJECT
PR:PROJECT_TITLE                 	2HG prodution in neurospheres from IDH1 mouse glioma model
PR:PROJECT_TYPE                  	MS analysis
PR:PROJECT_SUMMARY               	Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from
PR:PROJECT_SUMMARY               	the mice brain tumors haboring specific genetic lesions: NRAS overexpression,
PR:PROJECT_SUMMARY               	p53 knockdown plus or minus IDH1 mutated and ATRX knockdown.
PR:INSTITUTE                     	University of Michigan
PR:DEPARTMENT                    	Neurosurgery
PR:LABORATORY                    	Castro Lab
PR:LAST_NAME                     	Castro
PR:FIRST_NAME                    	Maria
PR:ADDRESS                       	Ann Arbor, MI
PR:EMAIL                         	mariacas@umich.edu
PR:PHONE                         	734-764-0850
#STUDY
ST:STUDY_TITLE                   	2 hydroxyglutarate prodution in neurospheres from IDH1 mouse glioma model #2
ST:STUDY_TYPE                    	MS analysis
ST:STUDY_SUMMARY                 	Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from
ST:STUDY_SUMMARY                 	the mice brain tumors haboring specific genetic lesions: NRAS overexpression,
ST:STUDY_SUMMARY                 	p53 knockdown plus or minus IDH1 mutated and ATRX knockdown.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	4
#SUBJECT
SU:SUBJECT_TYPE                  	MOUSE
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0016367	S00026214	IDH1_MUTATION:NO	
SUBJECT_SAMPLE_FACTORS           	SU0016367	S00026215	IDH1_MUTATION:NO	
SUBJECT_SAMPLE_FACTORS           	SU0016368	S00026216	IDH1_MUTATION:IDH1-R132H	
SUBJECT_SAMPLE_FACTORS           	SU0016368	S00026217	IDH1_MUTATION:IDH1-R132H	
SUBJECT_SAMPLE_FACTORS           	SU0016369	S00026218	IDH1_MUTATION:IDH1-R132H	
SUBJECT_SAMPLE_FACTORS           	SU0016369	S00026219	IDH1_MUTATION:IDH1-R132H	
SUBJECT_SAMPLE_FACTORS           	SU0016370	S00026220	IDH1_MUTATION:IDH1-R132H	
SUBJECT_SAMPLE_FACTORS           	SU0016370	S00026221	IDH1_MUTATION:IDH1-R132H	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	A037-2HG cells updated-20160721.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent
CH:COLUMN_NAME                   	Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	A037-2HG cells updated-20160721.pdf
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6490A QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	uM
MS_METABOLITE_DATA_START
Samples	S00026214	S00026215	S00026216	S00026217	S00026218	S00026219	S00026220	S00026221
Factors	IDH1_MUTATION:NO	IDH1_MUTATION:NO	IDH1_MUTATION:IDH1-R132H	IDH1_MUTATION:IDH1-R132H	IDH1_MUTATION:IDH1-R132H	IDH1_MUTATION:IDH1-R132H	IDH1_MUTATION:IDH1-R132H	IDH1_MUTATION:IDH1-R132H
(2R)-2-HYDROXYPENTANEDIOIC ACID	.25363048	.342549776	11.06987297	10.53820051	8.167143088	10.30796916	1.780790067	2.87467369
(2S)-2-HYDROXYPENTANEDIOIC ACID	.358184959	.340863541	0	0	0	0	.404508486	.538581489
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
(2R)-2-HYDROXYPENTANEDIOIC ACID				439391
(2S)-2-HYDROXYPENTANEDIOIC ACID				439939
METABOLITES_END
#END