#METABOLOMICS WORKBENCH araskind_20170802_131627 DATATRACK_ID:1176 STUDY_ID:ST000829 ANALYSIS_ID:AN001321 PROJECT_ID:PR000590 VERSION 1 CREATED_ON August 8, 2017, 12:14 pm #PROJECT PR:PROJECT_TITLE Mice Retina & Plasma Studies PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Control and 12W STZ B6 Mice Retina and Plasma PR:INSTITUTE University of Michigan PR:DEPARTMENT Ophthalmology & Visual Sciences PR:LABORATORY Abcouwer Lab PR:LAST_NAME Abcouwer PR:FIRST_NAME Steven PR:ADDRESS Ann Arbor, MI PR:EMAIL sabcouwe@umich.edu PR:PHONE 734-232-8227 #STUDY ST:STUDY_TITLE 12 weeks control & STZ B6 mice retina & plasma amino acid analysis ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Mice were injected with vehicle or STZ to induce diabetes. After 12 weeks, ST:STUDY_SUMMARY retinas and plasma were harvested. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 2 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012636 S00019142 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019143 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019144 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019145 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019146 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012637 S00019147 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019148 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019149 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019150 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019151 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019152 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019153 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019154 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019155 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019156 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012636 S00019157 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019158 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019159 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019160 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019161 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012637 S00019162 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019163 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019164 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019165 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019166 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019167 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019168 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019169 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019170 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019171 Sample type:Plasma | Type:12W STZ #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A005_Amino_Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A005 - Amino Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00019157 S00019158 S00019159 S00019160 S00019161 S00019162 S00019163 S00019164 S00019165 S00019166 S00019167 S00019168 S00019169 S00019170 S00019171 Factors Sample type:Plasma | Type:Control Sample type:Plasma | Type:Control Sample type:Plasma | Type:Control Sample type:Plasma | Type:Control Sample type:Plasma | Type:Control Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ Sample type:Plasma | Type:12W STZ L-PROLINE 40.61993724 22.32183617 38.01549219 45.09219288 29.3458431 36.09047393 41.31696125 59.07136921 55.48931556 34.80282878 26.64519471 40.91440211 35.34802578 63.36175763 45.18939862 L-GLUTAMIC ACID 10.78098443 10.91652363 12.17367097 17.46678542 15.60973145 10.99485464 12.50402282 8.89646888 8.557534158 3.224715899 4.6367922 6.424739037 6.765492962 8.113231448 11.39111083 L-ALANINE 176.8739724 125.2838437 198.6499401 214.3912914 144.5690588 190.8699122 184.8524096 219.1721867 260.764197 121.9565911 98.30946481 148.5426852 161.4965267 178.0700206 151.9569447 L-SERINE 16.57896624 17.76445556 35.85150512 38.92760397 25.43974376 35.85651662 36.82142061 38.39252703 72.10275983 36.76576803 28.79783438 26.01635513 32.22795007 63.57891583 42.58649444 L-ASPARTIC ACID 1.308806694 1.351247413 1.844263426 1.927355222 1.633531997 1.784402522 2.378226821 2.513874314 1.354954676 .412258662 .790464539 .842489442 .865524286 1.223425993 2.551528446 L-GLUTAMINE 4.140935914 8.152663117 8.012927588 L-LYSINE 97.06048004 106.783139 150.1656953 110.936258 91.0146733 58.51750124 76.38858654 73.6942849 58.40705572 32.00402203 29.58380482 25.79505481 25.24272214 102.9208797 56.08866517 L-TYROSINE 37.41526763 28.45427913 30.1172146 31.0181571 28.70958904 20.94388841 27.65814583 41.65054229 35.15764089 14.83627476 29.07380557 21.68845976 16.27334781 80.30021849 33.67936868 L-LEUCINE 68.48633511 34.06217041 47.51472926 64.19669809 47.01758965 67.93428008 98.98392942 116.8205429 187.79656 51.35256728 67.11385491 84.90549691 67.11549648 216.3285184 156.535194 2-AMINOISOBUTYRIC ACID 2.963241053 1.333498513 2.09231066 3.221657812 1.411861011 1.374212785 2.449745866 2.203698276 3.051558843 1.066780089 1.310611509 1.332468127 1.555369724 3.012408164 3.4606322 L-METHIONINE 14.65226498 6.838560192 14.87604432 13.65230568 11.03774101 8.627787716 13.26602534 23.81658834 26.08978836 7.036821227 3.869877439 5.453003294 4.213807478 41.5932986 11.78546398 L-PHENYLALANINE 27.39407982 22.07575717 24.15479362 28.11132449 25.38800708 25.41012467 29.32567674 29.88082916 33.65543371 18.12130086 19.62072404 20.06591437 21.5201888 40.83707644 28.14510136 L-ORNITHINE 18.39144708 17.81013858 22.81921146 26.59392455 16.5173662 20.90979775 23.31567812 32.74355375 23.67462381 7.174070822 9.291184644 10.43377154 13.1935425 31.32135599 17.22311005 L-ASPARAGINE 17.63197989 4.796622517 14.42740896 19.3969766 9.443504931 8.239099988 16.26814967 21.79670289 22.36888011 8.56159894 5.676498381 20.99813139 15.5186241 L-VALINE 143.9964676 83.17030003 97.51919296 127.9290484 91.70798867 107.4847824 177.6695613 162.822852 317.0808714 77.58234244 85.27751392 107.2768937 115.8897173 271.4082419 267.0590075 L-THREONINE 57.89627107 39.92979857 69.58040576 72.32522422 46.11105868 46.08079285 56.05549794 72.51648847 88.52513772 44.76371671 37.69116282 44.21232159 39.31510408 88.3940365 77.15415444 L-TRYPTOPHAN 3.11230765 12.21336199 14.46289428 14.30781344 12.31672365 5.800346247 11.70403374 8.462933989 23.84368811 9.263910066 4.647104526 4.870037851 6.312381775 6.156957908 4.388855904 L-ISOLEUCINE 52.88728818 28.28398609 33.39795773 45.4471672 36.74269305 45.09738448 67.35088447 76.494299 130.0657661 36.42551801 35.64650536 39.71537283 38.01355057 141.7818523 94.73362123 GLYCINE 92.76042683 68.27596575 112.9803821 113.3042751 89.56905547 74.53526431 81.48283244 78.36549166 115.8635949 71.34582346 59.76798149 77.86288643 71.8500794 96.28999921 106.0350383 4-HYDROXYPROLINE 3.903788138 4.192759241 5.317962921 3.751833708 3.25562901 2.405008929 3.883884211 3.691598591 1.202856351 1.374326002 4.509427697 3.73136959 8.204177141 8.003231897 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type L-PROLINE 145742 L-GLUTAMIC ACID 33032 L-ALANINE 5950 L-SERINE 5951 L-ASPARTIC ACID 5960 L-GLUTAMINE 5961 L-LYSINE 5962 L-TYROSINE 6057 L-LEUCINE 6106 2-AMINOISOBUTYRIC ACID 6119 L-METHIONINE 6137 L-PHENYLALANINE 6140 L-ORNITHINE 6262 L-ASPARAGINE 6267 L-VALINE 6287 L-THREONINE 6288 L-TRYPTOPHAN 6305 L-ISOLEUCINE 6306 GLYCINE 750 4-HYDROXYPROLINE 825 METABOLITES_END #END