#METABOLOMICS WORKBENCH araskind_20170802_131627 DATATRACK_ID:1176 STUDY_ID:ST000829 ANALYSIS_ID:AN001322 PROJECT_ID:PR000590 VERSION 1 CREATED_ON August 8, 2017, 12:14 pm #PROJECT PR:PROJECT_TITLE Mice Retina & Plasma Studies PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Control and 12W STZ B6 Mice Retina and Plasma PR:INSTITUTE University of Michigan PR:DEPARTMENT Ophthalmology & Visual Sciences PR:LABORATORY Abcouwer Lab PR:LAST_NAME Abcouwer PR:FIRST_NAME Steven PR:ADDRESS Ann Arbor, MI PR:EMAIL sabcouwe@umich.edu PR:PHONE 734-232-8227 #STUDY ST:STUDY_TITLE 12 weeks control & STZ B6 mice retina & plasma amino acid analysis ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Mice were injected with vehicle or STZ to induce diabetes. After 12 weeks, ST:STUDY_SUMMARY retinas and plasma were harvested. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 2 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012636 S00019142 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019143 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019144 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019145 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019146 Sample type:Retina | Type:Control SUBJECT_SAMPLE_FACTORS SU0012637 S00019147 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019148 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019149 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019150 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019151 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019152 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019153 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019154 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019155 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019156 Sample type:Retina | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012636 S00019157 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019158 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019159 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019160 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012636 S00019161 Sample type:Plasma | Type:Control SUBJECT_SAMPLE_FACTORS SU0012637 S00019162 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019163 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019164 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019165 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019166 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019167 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019168 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019169 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019170 Sample type:Plasma | Type:12W STZ SUBJECT_SAMPLE_FACTORS SU0012637 S00019171 Sample type:Plasma | Type:12W STZ #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A005_Amino_Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A005 - Amino Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS umol/ug protein MS_METABOLITE_DATA_START Samples S00019142 S00019143 S00019144 S00019145 S00019146 S00019147 S00019148 S00019149 S00019150 S00019151 S00019152 S00019153 S00019154 S00019155 S00019156 Factors Sample type:Retina | Type:Control Sample type:Retina | Type:Control Sample type:Retina | Type:Control Sample type:Retina | Type:Control Sample type:Retina | Type:Control Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ Sample type:Retina | Type:12W STZ L-PROLINE 14.88634548 13.08498934 11.28146605 13.37990443 13.03662856 8.655209233 5.090312364 9.140499703 8.02742126 9.352114734 7.257500177 7.988158519 7.83003916 12.20634205 16.69451792 L-GLUTAMIC ACID 438.6110514 402.3212747 274.2350892 582.9657449 557.2620067 253.1371651 140.0657911 304.9224212 284.5773845 249.3906734 174.6553012 256.3710951 280.8816617 261.2049929 423.352736 L-ALANINE 88.41548236 75.75932353 68.06453584 120.7955396 118.8833243 87.39097181 36.5448996 86.55474007 101.5768521 86.2206387 66.02561939 96.40466237 97.11057275 127.3212962 112.422377 L-SERINE 12.19195625 11.47791645 9.756755052 8.025154606 15.75570352 12.37481621 6.736438455 7.035030913 2.594475571 14.16025173 6.614599657 6.161121398 7.833820681 L-ASPARTIC ACID 204.6416746 163.7621484 85.43732087 108.7132153 102.5124792 58.43256623 27.10690728 56.66759985 39.72906003 41.55894027 43.8351775 47.93464702 40.60979441 56.46976448 90.88479881 L-GLUTAMINE 57.45113032 73.45529252 68.48398721 120.0806869 85.67479435 89.09003909 67.27683226 84.31429496 21.19597918 192.7296644 47.66885301 1620.497748 43.16285704 38.54482293 63.78549273 L-LYSINE 7.971751976 4.929831109 4.404144026 5.044383675 5.142051711 3.788960431 2.187319267 3.342393232 3.221764102 4.664651744 3.110350153 4.41916798 2.915304791 3.196826539 3.762513905 L-TYROSINE 8.189783425 6.232481179 7.59195256 8.38862142 7.664316368 3.286929113 2.17950447 5.000745486 1.576345487 4.551866836 1.113038897 2.853305148 4.166399696 2.851963984 4.37671959 L-LEUCINE 15.15347574 18.86898716 24.95833255 18.20413421 14.0815757 10.54615371 7.923886792 12.75290074 12.5966569 14.4499645 8.562024264 10.39508337 11.32157035 17.69179543 26.03219864 L-METHIONINE 4.662396946 3.20192897 2.700794526 4.614480361 2.00717989 .864992178 .598539045 2.07125023 1.161562944 3.043170798 .647508857 .094586957 L-PHENYLALANINE 7.897173355 11.75988474 9.136787352 9.855005703 9.299439958 5.839774983 2.982135454 4.756624928 3.827403338 6.666813303 4.576910766 4.029438628 4.5946142 5.329432246 7.015566977 L-ORNITHINE .624457075 .178870196 .131226701 .151918824 L-ASPARAGINE 5.54425224 9.88281078 3.820020193 5.937533958 2.733724306 3.627327956 1.448633207 2.041495694 4.016426316 3.938557772 2.357655117 3.713229033 L-HISTIDINE 3.861110922 4.238216142 9.330059279 7.590051851 L-VALINE 17.95206555 19.97483575 16.68039167 22.56682089 16.41244823 12.60713701 9.970648351 14.39137396 15.11899908 16.67257543 10.12185467 12.57379634 13.30381494 21.41481778 28.99809259 L-THREONINE 10.80397773 10.78881039 12.20508168 7.584028811 14.30714726 8.588410836 5.163713696 8.487415317 4.416355042 7.557328981 6.467782941 2.331592378 7.428903847 9.011045254 L-TRYPTOPHAN 1.720387192 2.695001001 2.959967773 2.970851105 1.881738987 .68607662 .402485827 1.062244133 .211768111 1.445995086 .160906135 .67434473 .57313733 .199156067 .522220825 L-ISOLEUCINE 9.636647862 9.766844053 8.270683806 10.87454231 8.052288305 7.325297046 4.890107547 6.424462992 8.078918607 8.978957119 5.539125035 5.508183869 6.943394931 9.321655614 13.37594883 GLYCINE 264.565229 212.8631941 156.5359538 203.4643077 217.4738981 142.0529415 68.95146468 127.5343621 126.9118032 127.2738887 132.057156 141.9703038 150.8986211 179.0922406 241.9594156 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type L-PROLINE 145742 L-GLUTAMIC ACID 33032 L-ALANINE 5950 L-SERINE 5951 L-ASPARTIC ACID 5960 L-GLUTAMINE 5961 L-LYSINE 5962 L-TYROSINE 6057 L-LEUCINE 6106 L-METHIONINE 6137 L-PHENYLALANINE 6140 L-ORNITHINE 6262 L-ASPARAGINE 6267 L-HISTIDINE 6274 L-VALINE 6287 L-THREONINE 6288 L-TRYPTOPHAN 6305 L-ISOLEUCINE 6306 GLYCINE 750 METABOLITES_END #END